CN110684073A - Preparation method of low-THC organic fructus cannabis protein powder - Google Patents
Preparation method of low-THC organic fructus cannabis protein powder Download PDFInfo
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- CN110684073A CN110684073A CN201911134529.1A CN201911134529A CN110684073A CN 110684073 A CN110684073 A CN 110684073A CN 201911134529 A CN201911134529 A CN 201911134529A CN 110684073 A CN110684073 A CN 110684073A
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Abstract
The invention discloses a preparation method of low-THC organic fructus cannabis protein powder, and belongs to the field of vegetable protein extraction. The invention solves the problems that the existing method for extracting protein powder from fructus cannabis has complex preparation process and low product purity, and the THC contained in the protein powder is easy to have poisoning hidden trouble. The invention comprises the following steps: removing impurities and foreign matters in the hemp seeds, and mechanically crushing by adopting a rail grinding type crusher; cold pressing at low temperature, crushing, sieving, enzymolysis, enzyme deactivation, three-phase separation, cavitation jet treatment, pressure direct-injection sterilization, falling film concentration and centrifugal spray drying. In the preparation process, THC is destroyed to a great extent by adopting pressure direct-injection sterilization, jet cavitation treatment is applied, and the THC is treated under the action of high pressure, shearing and cavitation, so that the THC product is not detected. The method has the advantages of simple process, high automation degree and low production cost, and greatly improves the extraction rate of the hemp protein; the obtained hemp protein has low denaturation degree, and good gel property and emulsibility.
Description
Technical Field
The invention relates to a preparation method of low-THC organic fructus cannabis protein powder, and belongs to the field of vegetable protein extraction.
Background
Induce diuresis to treat stranguria and activate blood circulation, it is a moistening-purgative herb that is also eaten as a herb. Modern researches show that the fructus cannabis has better effects of reducing blood pressure, relieving pain, resisting inflammation and resisting thrombosis, and has good auxiliary curative effects on chronic neuritis, paralysis, ulcer and gynecological diseases after being eaten for a long time. The fructus cannabis cake meal is residue obtained by extracting fructus cannabis oil from fructus cannabis, has protein content as high as 56%, contains various essential amino acids required by human body, is particularly rich in arginine, lysine and glutamic acid, and is an excellent plant protein
The fructus cannabis contains phenolic substances which are very easy to oxidize, and the phenolic substances can remain in the fructus cannabis cake after the fructus cannabis is processed, and pink oxidation products can be generated after the fructus cannabis cake is oxidized in the air, so that the color and luster of products processed by the fructus cannabis cake are influenced. In addition, the hemp seed cake contains a part of monosaccharide besides protein, so that the separation process of the hemp seed protein is complicated. In addition, the separated fructus cannabis protein contains residual THC (tetrahydrocannabinol), excessive use of THC is easy to cause toxic reaction, mild patients show dizziness, thirst and mouth numbness, and severe patients can cause coma, deep sleep and the like, so that the wide application and deep development of the fructus cannabis protein are greatly influenced. The preparation method of the low-THC organic fructus cannabis protein powder is necessary for solving the problems that the existing method for extracting protein powder from fructus cannabis is complex in preparation process, low in product purity and easy to cause poisoning hidden trouble due to THC contained in the fructus cannabis.
Disclosure of Invention
The invention provides low-THC organic fructus cannabis protein powder, aiming at solving the problems that the existing method for extracting protein powder from fructus cannabis is complex in preparation process, low in product purity and easy to cause poisoning hidden trouble due to THC contained in the fructus cannabis.
The technical scheme of the invention is as follows:
the method for preparing the low-THC organic fructus cannabis protein powder comprises the following operation steps:
removing impurities and foreign matters in the hemp seeds, and then mechanically crushing to obtain hemp seed particles;
step two, carrying out low-temperature cold pressing on the hemp seed particles (the whole low-temperature cold pressing process is carried out below 35 ℃), after oil residue separation, crushing and sieving cold pressing residues, carrying out enzymolysis and enzyme deactivation, and carrying out three-phase separation on the mixture after enzyme deactivation to obtain hemp seed oil, a solution containing THC and hemp protein and residues;
and step three, carrying out cavitation jet treatment on the solution containing the THC and the hemp protein obtained in the step two, sterilizing by adopting a pressure direct injection mode, carrying out falling film concentration, and carrying out centrifugal spray drying to obtain the hemp protein powder.
Preferably: in the first step, after impurities and foreign matters are removed from the cannabis sativa seeds, the sum of the mass of the impurities and the mass of the imperfect seeds is less than 0.2 percent; the obtained hemp seed particles are crushed mechanically, and the granularity is 10-20 meshes.
Preferably: the granularity of the cold-pressed slag crushed in the second step is 100-120 meshes.
Preferably: the enzymolysis conditions in the second step are as follows: the mass ratio of the cold pressing slag to the water is 1: (8-10), the enzymolysis temperature is 45-65 ℃, the enzymolysis PH is 8.0-10.0, and the enzymolysis time is 60-80 min.
Preferably: the enzyme deactivation conditions in the second step are as follows: the enzyme deactivation temperature is 80-90 ℃, and the enzyme deactivation time is 1-2 min.
Most preferably: the enzyme for enzymolysis is protease Alcalase 2.4L.
Preferably: the cavitation jet treatment conditions in the third step are as follows: the pressure is 0.32MPa-0.36MPa, and the treatment time is 120min-150 min.
Preferably: the pressure direct injection type sterilization conditions in the third step are as follows: the steam pressure is 60MPa-80MPa, the sterilization temperature is 138 ℃ to 142 ℃, and the sterilization time is 5s-7 s.
Preferably: in the falling film concentration process in the third step, the solution containing THC and hemp protein is concentrated to 38-40 Baume degrees.
Preferably: the centrifugal spray drying conditions in the third step are as follows: the air inlet temperature is 180-200 ℃, the air outlet temperature is 80-90 ℃, and the frequency is 200-300 Hz.
The invention has the following beneficial effects: the preparation process of the invention does not use any solvent in the whole preparation process, and completely meets the organic protein standards of European Union, America, Japan and China; in the preparation process, THC is greatly destroyed by adopting pressure direct-injection sterilization, jet cavitation treatment is applied, and the THC content of the obtained product is 0 at least under the action of high pressure, shearing and cavitation. In addition, the method has the advantages of simple process, high automation degree and low production cost, greatly improves the extraction rate of the hemp protein, simultaneously ensures that the denaturation degree of the hemp protein is low, and ensures that the hemp protein has good gelling property and emulsifying property.
Detailed Description
The experimental procedures used in the following examples are conventional unless otherwise specified.
Embodiment mode 1:
the method comprises the following steps: removing impurities and foreign matters in the hemp seeds, and mechanically crushing the hemp seeds by adopting a rolling rail type crusher to obtain hemp seed particles with the granularity of 10 meshes;
step two: cold pressing the hemp seed particles at low temperature, separating oil residues, crushing and sieving cold pressing residues to obtain cold pressing residue particles with the granularity of 100 meshes, performing enzymolysis on the cold pressing residue particles by using protease Alcalase 2.4L, wherein the enzymolysis conditions are as follows: the mass ratio of cold pressing slag to water is 1:8, the enzymolysis temperature is 45 ℃, the enzymolysis PH is 8.0, and the enzymolysis time is 60 min; and (3) after the enzymolysis is finished, carrying out enzyme deactivation at the temperature of 80 ℃ for 1min, and carrying out three-phase separation on the mixture after the enzyme deactivation to obtain cannabis sativa oil, a solution containing THC and cannabis sativa protein and residues.
Step three: performing cavitation jet treatment on the solution containing the THC and the hemp protein obtained in the step two, wherein the pressure of the cavitation jet treatment is 0.32MPa, and the treatment time is 120 min; then, sterilizing by adopting a pressure direct injection mode, wherein the steam pressure of sterilization is 60MPa, the sterilization temperature is 138 ℃, and the sterilization time is 5 s; then using falling film concentration, concentrating until the hemp protein liquid is 38 Baume degrees, carrying out centrifugal spray drying at the air inlet temperature of 180 ℃, the air outlet temperature of 80 ℃ and the frequency of 200Hz, and obtaining the hemp protein powder after drying. The content of THC in the fructus Cannabis protein powder is 0.03%, and extraction rate of fructus Cannabis protein is 90.13%.
Embodiment mode 2:
the method comprises the following steps: removing impurities and foreign matters in the hemp seeds, and mechanically crushing the hemp seeds by adopting a rolling rail type crusher to obtain hemp seed particles with the particle size of 15 meshes;
step two: cold pressing the hemp seed particles at low temperature, separating oil residues, crushing and sieving cold pressing residues to obtain cold pressing residue particles with the granularity of 110 meshes, performing enzymolysis on the cold pressing residue particles by using protease Alcalase 2.4L, wherein the enzymolysis conditions are as follows: the mass ratio of cold pressing slag to water is 1:9, the enzymolysis temperature is 50 ℃, the enzymolysis PH is 9.0, and the enzymolysis time is 70 min; and (3) after the enzymolysis is finished, carrying out enzyme deactivation at the temperature of 85 ℃ for 1.5min, and carrying out three-phase separation on the mixture after the enzyme deactivation to obtain hemp seed oil, a solution containing THC and hemp protein and residues.
Step three: performing cavitation jet treatment on the solution containing the THC and the hemp protein obtained in the step two, wherein the pressure of the cavitation jet treatment is 0.34MPa, and the treatment time is 135 min; then, sterilizing by adopting a pressure direct injection mode, wherein the steam pressure of sterilization is 70MPa, the sterilization temperature is 140 ℃, and the sterilization time is 6 s; and then falling film concentration is carried out, centrifugal spray drying is carried out after the concentration is carried out until the hemp protein liquid is 39 Baume degrees, the air inlet temperature is 190 ℃, the air outlet temperature is 85 ℃, the frequency is 250Hz, and the hemp protein powder is obtained after drying is finished. The content of THC in the fructus cannabis protein powder is 0, and the extraction rate of fructus cannabis protein is 93.25%.
Embodiment mode 3:
the method comprises the following steps: removing impurities and foreign matters in the hemp seeds, and mechanically crushing the hemp seeds by adopting a rolling rail type crusher to obtain hemp seed particles with the granularity of 20 meshes;
step two: cold pressing the hemp seed particles at low temperature, separating oil residues, crushing and sieving cold pressing residues to obtain cold pressing residue particles with the granularity of 120 meshes, performing enzymolysis on the cold pressing residue particles by using protease Alcalase 2.4L, wherein the enzymolysis conditions are as follows: the mass ratio of cold pressing slag to water is 1:10, the enzymolysis temperature is 65 ℃, the enzymolysis PH is 10.0, and the enzymolysis time is 80 min; and (3) after the enzymolysis is finished, carrying out enzyme deactivation at the temperature of 90 ℃ for 2min, and carrying out three-phase separation on the mixture after the enzyme deactivation to obtain cannabis sativa oil, a solution containing THC and cannabis sativa protein and residues.
Step three: performing cavitation jet treatment on the solution containing the THC and the hemp protein obtained in the step two, wherein the pressure of the cavitation jet treatment is 0.36MPa, and the treatment time is 150 min; then, sterilizing by adopting a pressure direct injection mode, wherein the steam pressure of sterilization is 80MPa, the sterilization temperature is 142 ℃, and the sterilization time is 7 s; and then falling film concentration is carried out, centrifugal spray drying is carried out after the concentration is carried out until the hemp protein liquid is 40 Baume degrees, the air inlet temperature is 200 ℃, the air outlet temperature is 90 ℃, the frequency is 300Hz, and the hemp protein powder is obtained after drying is finished. The content of THC in the fructus Cannabis protein powder is 0, and extraction rate of fructus Cannabis protein is 93.35%.
Claims (10)
1. A preparation method of low-THC organic fructus cannabis protein powder is characterized by comprising the following steps: the method comprises the following steps:
removing impurities and foreign matters in the hemp seeds, and mechanically crushing to obtain hemp seed particles;
step two, cold pressing the hemp seed particles at low temperature, separating oil residues, crushing and screening cold pressing residues, performing enzymolysis, deactivating enzyme, and performing three-phase separation on a mixture after enzyme deactivation to obtain hemp seed oil, a solution containing THC and hemp protein and residues;
and step three, carrying out cavitation jet treatment on the solution containing the THC and the hemp protein obtained in the step two, sterilizing by adopting a pressure direct injection mode, carrying out falling film concentration, and carrying out centrifugal spray drying to obtain the hemp protein powder.
2. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: after impurities and foreign matters are removed from the hemp seeds in the first step, the sum of the mass of the impurities and the imperfect hemp seeds is less than 0.2 percent; the obtained hemp seed particles are crushed mechanically, and the granularity is 10-20 meshes.
3. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: the granularity of the cold-pressed slag crushed in the second step is 100-120 meshes.
4. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: the enzymolysis conditions in the second step are as follows: the mass ratio of the cold pressing slag to the water is 1: (8-10), the enzymolysis temperature is 45-65 ℃, the enzymolysis pH is 8.0-10.0, and the enzymolysis time is 60-80 min.
5. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: the enzyme deactivation conditions in the second step are as follows: the enzyme deactivation temperature is 80-90 ℃, and the enzyme deactivation time is 1-2 min.
6. The method for preparing the low-THC organic fructus cannabis protein powder as claimed in any one of claims 1 or 4, wherein the method comprises the following steps: the enzyme for enzymolysis is protease Alcalase 2.4L.
7. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: the cavitation jet treatment conditions in the third step are as follows: the pressure is 0.32MPa-0.36MPa, and the treatment time is 120min-150 min.
8. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: the pressure direct injection type sterilization conditions in the third step are as follows: the steam pressure is 60MPa-80MPa, the sterilization temperature is 138 ℃ to 142 ℃, and the sterilization time is 5s-7 s.
9. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: in the falling film concentration process in the third step, the solution containing THC and hemp protein is concentrated to 38-40 Baume degrees.
10. The preparation method of the low-THC organic fructus cannabis protein powder as claimed in claim 1, and is characterized in that: the centrifugal spray drying conditions in the third step are as follows: the air inlet temperature is 180-200 ℃, the air outlet temperature is 80-90 ℃, and the frequency is 200-300 Hz.
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Cited By (1)
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| CN114258960A (en) * | 2021-12-21 | 2022-04-01 | 吉林农业大学 | Detoxified hemp seed cheese |
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