CN110672688A - Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether and preparation method and application thereof - Google Patents
Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether and preparation method and application thereof Download PDFInfo
- Publication number
- CN110672688A CN110672688A CN201910817348.2A CN201910817348A CN110672688A CN 110672688 A CN110672688 A CN 110672688A CN 201910817348 A CN201910817348 A CN 201910817348A CN 110672688 A CN110672688 A CN 110672688A
- Authority
- CN
- China
- Prior art keywords
- electrode
- zif
- mwnts
- cile
- hydroxyethyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Abstract
The invention discloses an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether, and a preparation method and application thereof. The electrochemical biosensor takes a carbon ion liquid electrode as a substrate electrode, and a zinc metal-organic framework nano material layer, a chlorine-doped multi-wall carbon nanotube layer, a monoclonal antibody layer and a Nafion film are sequentially modified on the surface of the substrate electrode. The preparation method of the electrochemical biosensor comprises the steps of modifying ZIF-8 on the surface of CILE, electrodepositing Cl2-MWNTs, assembling a monoclonal antibody, and finally covering a Nafion membrane. The nano electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether has wider detection range and low detection limit, and can be used for detecting a sample containing tetrabromobisphenol A bis (2-hydroxyethyl) ether.
Description
Technical Field
The invention relates to the technical field of nano materials, electrochemical detection and biosensors, and particularly relates to an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether, and a preparation method and application thereof.
Background
The electrochemical biosensor refers to a sensor using an immobilized biomaterial as a recognition element. The working principle of the sensor is that biological components (enzyme, polypeptide, protein, antibody, antigen and the like) or organisms (cells, tissues, organelles and the like) are used as recognition elements, electrodes such as fixed electrodes, ion selective electrodes, gas sensitive electrodes and the like are used as conversion elements, target molecules are selectively recognized and captured on the surfaces of the electrodes through the specific recognition effect among biological molecules, then signals of basic electrodes are derived, and signals are detected by taking potential, current, capacitance or conductance as characteristics. Electrochemical biosensors have an important weight in biosensors, and research work thereof has been rapidly progressed in recent years.
Tetrabromobisphenol A bis (2-hydroxyethyl) ether (TBBPA DHEE) is a typical TBBPA derivative which has wide application, but has potential neurotoxicity and strongest cytotoxicity. On the surface of research, TBBPA DHEE can enter the environment through various ways, and finally enters human bodies through food chain enrichment, thereby causing certain harm to human health. Therefore, the method is very necessary to realize real-time detection of TBBPA DHEE and investigate the pollution condition and characteristics of the TBBPA DHEE.
Patent CN109916979A discloses a tetrabromobisphenol A molecularly imprinted electrochemical sensor, which comprises a working electrode consisting of a glassy carbon electrode, a multi-wall carbon nanotube film positioned on the surface of the working electrode, gold nanoparticles and an electropolymerized molecularly imprinted polymer film, a reference electrode Ag/AgCl, a counter electrode platinum wire and an electrolyte, and can be used for direct electrochemical detection of tetrabromobisphenol A. However, tetrabromobisphenol a bis (2-hydroxyethyl) ether cannot be detected, so that a novel nano electrochemical biosensor is needed to be developed, and tetrabromobisphenol a bis (2-hydroxyethyl) ether (TBBPADHEE) can be rapidly and accurately detected.
Disclosure of Invention
The present invention is directed to the above-mentioned drawbacks and disadvantages of the prior art and provides an electrochemical biosensor for detecting tetrabromobisphenol a bis (2-hydroxyethyl) ether.
The invention also aims to provide a preparation method of the electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether.
Still another object of the present invention is to provide an application of the electrochemical biosensor for detecting tetrabromobisphenol a bis (2-hydroxyethyl) ether.
The above object of the present invention is achieved by the following technical solutions:
an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether uses a Carbon Ion Liquid Electrode (CILE) as a substrate electrode, and a zinc metal-organic framework nano material (ZIF-8) layer and a chlorine-doped multi-walled carbon nanotube (Cl) are sequentially modified on the surface of the substrate electrode2MWNTs), monoclonal antibody layer and Nafion membrane. Namely the ZIF-8, Cl2-MWNTs and monoclonal antibodies are arranged on the CILE surface from inside to outside to form a composite membrane.
In the electrochemical biosensor, ZIF-8 is a porous material, and has a stable internal space structure and a high specific surface area. Electrodeposited Cl2MWNTs can be uniformly deposited on the surface of the three-dimensional structure of the ZIF-8; cl electrodeposited on porous ZIF-8 channels and electrode surfaces2MWNTs provides a good conductive platform for the specific binding of monoclonal antibodies, and finally, a Nafion film is used for curing the composite material, so that the electrode is more stable and is beneficial to charge transmission, the stability of the sensor is improved, and the service life of the sensor is prolonged. The zinc metal-organic framework material (ZIF-8) is formed by assembling Zn (II) ions and a ligand 2-methylimidazole, has a dodecahedral structure with a three-dimensional structure, has the characteristic of a typical small-aperture macroporous cage, and has the advantages of excellent adsorption capacity, high specific surface area, more catalytic sites, high mechanical strength, high chemical stability and the like. However, the ZIF-8 has limited conductivity, so that the application of the ZIF-8 in the electrochemical field is limited, and therefore, the composite material formed by introducing the chlorine-doped multi-walled carbon nano-tube with high conductivity effectively overcomes the defect of the ZIF-8 material. Chlorine doped multi-walled carbon nanotubes (Cl)2MWNTs) is a composite material prepared by doping multiwall carbon nanotubes with chlorine. It has large specific surface area, large length-diameter ratio, high conductivity and high conductivityThe material has the characteristics of heat property, compact structure and the like, and can be applied to the preparation of electrochemical electrodes due to the advantages of high specific surface area, high conductivity and the like. When TBBPA DHEE exists in a sample to be detected, the polyclonal antibody on the surface of the electrode can be specifically combined with the TBBPA DHEE, so that current change is generated. The ZIF-8, Cl2The synergistic amplification effect of MWNTs and the monoclonal antibody effectively improves the electrochemical performance of the sensor and improves the selectivity and the sensitivity of the electrode.
The preparation method of the electrochemical biosensor comprises the following steps:
s1, modifying ZIF-8: dripping 0.2-0.6 mg/mL ZIF-8 suspension on the surface of a Carbon Ion Liquid Electrode (CILE) to obtain a CILE modified by ZIF-8;
s2, electrodepositing Cl2-MWNTs: electrodepositing chlorine-doped multi-walled carbon nanotubes (Cl) on the surface of the electrode obtained in step S12MWNTs) to yield Cl2-MWNTs/ZIF-8/CILE；
S3, modifying the monoclonal antibody: dripping 12-18 mg/mL monoclonal antibody solution on the surface of the electrode obtained in the step S2 to obtain monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE；
S4, covering a Nafion membrane: dripping Nafion ethanol solution on the surface of the electrode obtained in the step S3 to ensure that all electrode materials can be stably fixed on the surface of the electrode, and obtaining the nano electrochemical biosensor; namely Nafion/monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE。
Preferably, in step S2, to apply cyclic voltammetry, the initial voltage and high potential are set to 0.5V, the low potential is-1.5V, and the electrodeposition Cl is cyclically scanned in the interval2MWNTs, number of scanning turns 10 turns, and the whole electrodeposition process is in nitrogen atmosphere.
Preferably, the modification of the step S1 is to drop 4-8 μ L (preferably 6 μ L) of ZIF-8 suspension at 0.2-0.6 mg/mL (preferably 0.4mg/mL) of CILE surface.
Preferably, the step S3 modified polyclonal antibody is Cl obtained in step S22And dripping 8-12 mu L (preferably 10 mu L) of monoclonal antibody solution of 15mg/mL on the surface of the MWNTs/ZIF-8/CILE electrode. The monoclonal antibody is used for specifically detecting tetrabromobisphenol A bis (2-hydroxyethyl)) Monoclonal antibodies to ethers. Specifically, the monoclonal antibody is a tetrabromobisphenol A bis (2-hydroxyethyl) ether monoclonal antibody which is obtained by preparing tetrabromobisphenol A bis (2-hydroxyethyl) ether immunogen and culturing mouse hybridoma cells in vivo or is commercially available.
Preferably, step S4 is the monoclonal antibody/Cl obtained in step S3260-100 mu L (preferably 80 mu L) of Nafion ethanol solution (with the concentration of 0.5%) is dripped on the surface of MWNTs/ZIF-8/CILE, so that the electrode material can be stably fixed on the surface of an electrode, and the preparation of the nano electrochemical biosensor is finished, namely Nafion/monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE。
Preferably, step S4 is drying at 4 deg.C to obtain Nafion/monoclonal antibody/Cl2MWNTs/ZIF-8/CILE electrode.
The invention also requests the application of the electrochemical biosensor in the detection of tetrabromobisphenol A bis (2-hydroxyethyl) ether.
Specifically, the electrochemical biosensor is used as a working electrode, a platinum sheet electrode is used as a counter electrode, an Ag/AgCl electrode is used as a reference electrode, a Tris-HCl buffer solution with the pH value of 8 is used as a supporting electrolyte, a three-electrode system is established, and the concentration of tetrabromobisphenol A bis (2-hydroxyethyl) ether in a sample is calculated according to the change relation between the current and the concentration of tetrabromobisphenol A bis (2-hydroxyethyl) ether by adopting a cyclic voltammetry method.
Preferably, in the step of detecting TBBPA DHEE, the pH value of the Tris-HCl buffer solution is 5.0-9.0, and the pH value of the buffer solution is 8.0.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether, which is prepared by using ZIF-8 and Cl2The synergistic amplification effect of MWNTs and the monoclonal antibody effectively improves the electrochemical performance of the sensor and improves the selectivity and the sensitivity of the electrode; the electrochemical biosensor has a wider detection range (0.6 ng/mL-4.5 ng/mL) and a lower detection limit (0.25ng/mL), and the preparation method of the electrochemical biosensor is simple in process and finishedThe method has the advantages of low cost, convenience in operation, no pollution, wide range and the like, and can realize the specific detection of TBBPA DHEE.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1
A preparation method of an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether comprises the following steps:
(1) and (3) putting 1.6g of graphite powder and 0.8g of N-hexyl pyridine hexafluorophosphate (HPPF6) into a mortar, grinding for 3h to obtain carbon paste, filling the carbon paste into a glass electrode tube with the inner diameter of 4mm, compacting, and inserting a copper wire as a lead to obtain the carbon ion liquid electrode CILE.
(2) Modifying ZIF-8: and (3) dripping 6 mu L of 0.4mg/mL ZIF-8 suspension on the surface of the CILE obtained in the step (2) to obtain the ZIF-8 modified CILE, namely ZIF-8/CILE.
(3) Electrodeposition of Cl2-MWNTs: soaking the ZIF-8/CILE electrode obtained in the step (2) in 1mg/mL Cl2in-MWNTs solution, cyclic voltammetry is adopted, initial voltage and high potential are set to be 0.5V, low potential is-1.5V, and Cl is electrodeposited in an interval in a cyclic scanning mode2MWNTs are arranged on the surface of the ZIF-8/CILE electrode, the number of scanning turns is 10, and the whole electrodeposition process is carried out in a nitrogen atmosphere. Washing with ultrapure water, standing and drying to obtain Cl2-MWNTs/ZIF-8/CILE；
(4) Preparation of monoclonal antibody: reference is made to the literature (Zhang Z, Zhu N, Huang M, et al. sensitive immunological assay for hybridoma A bis (2-hydroxyethyl) ether and hybridoma A mono (hydroxyethyl) ether: antibiotic and regenerative strategy to estimate the systemic immunological ether), tetrabromobisphenol A bis (2-hydroxyethyl) ether immunogen is synthesized using conventional immunological procedures (CuiJ, Zhang K, anang Q, animal immunological assay) 2017,229: 431-438-atmosphere), hybridoma cells are obtained by inoculating hybridoma cells in a chamber of a hybridoma cell culture (2-hydroxyethyl) and hybridoma cells in a chamber of a hybridoma cell culture medium (J. Environmental amplification, 3584. bovine lymphoma) and hybridoma cells obtained by culturing the hybridoma cells in a chamber of a hybridoma cell culture medium (bovine lymphoma) culture K, Huang M, et al. antibiotic infection, bovine lymphoma cells in a chamber of a hybridoma cell culture medium (2-bovine lymphoma) and hybridoma cell culture medium (2-bovine lymphoma cells) in the presence of hybridoma cell culture medium (1. hybridoma cell culture medium) and hybridoma cell culture medium (bovine lymphoma cells in a chamber of a hybridoma cell culture medium (hybridoma cell culture medium, and producing a large amount of ascites, obtaining a large amount of monoclonal antibodies from the ascites, and separating and purifying to obtain the monoclonal antibodies.
(5) Modified monoclonal antibodies: cl in said step (3)2Dripping 10 mu L of 15mg/mL monoclonal antibody solution on the surface of the-MWNTs/ZIF-8/CILE electrode to obtain monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE；
(6) Covering with a Nafion film: the monoclonal antibody/Cl obtained in said step (4)280 mu L of 0.5% Nafion ethanol solution is dripped on the surface of the MWNTs/ZIF-8/CILE electrode, so that the electrode material can be stably fixed on the electrode, and the preparation of the nano electrochemical biosensor is finished, namely Nafion/monoclonal antibody/Cl2MWNTs/ZIF-8/CILE electrode.
Example 2
A preparation method of an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether comprises the following steps:
(1) and (3) putting 1.6g of graphite powder and 0.8g of N-hexyl pyridine hexafluorophosphate (HPPF6) into a mortar, grinding for 3h to obtain carbon paste, filling the carbon paste into a glass electrode tube with the inner diameter of 4mm, compacting, and inserting a copper wire as a lead to obtain the carbon ion liquid electrode CILE.
(2) Modifying ZIF-8: and (3) dripping 4 mu L of 0.6mg/mL ZIF-8 suspension on the surface of the CILE obtained in the step (2) to obtain the ZIF-8 modified CILE, namely ZIF-8/CILE.
(3) Electrodeposition of Cl2-MWNTs: soaking the ZIF-8/CILE electrode obtained in the step (2) in 1mg/mL Cl2In MWNTs solution, using cyclic voltammetry, initial voltage and highPotential setting bit of 0.5V and low potential of-1.5V, and cyclic scanning of electrodeposited Cl in interval2MWNTs are arranged on the surface of the ZIF-8/CILE electrode, the number of scanning turns is 10, and the whole electrodeposition process is carried out in a nitrogen atmosphere. Washing with ultrapure water, standing and drying to obtain Cl2-MWNTs/ZIF-8/CILE；
(4) Modified monoclonal antibodies: cl in said step (3)2Dripping 8 mu L of 15mg/mL monoclonal antibody solution on the surface of the-MWNTs/ZIF-8/CILE electrode to obtain monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE；
(5) Covering with a Nafion film: the monoclonal antibody/Cl obtained in said step (4)2Dripping 100 mu L of 0.5% Nafion ethanol solution on the surface of the MWNTs/ZIF-8/CILE electrode to ensure that the electrode material can be stably fixed on the electrode, and finishing the preparation of the nano electrochemical biosensor, namely Nafion/monoclonal antibody/Cl2MWNTs/ZIF-8/CILE electrode.
Example 3
A preparation method of an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether comprises the following steps:
(1) and (3) putting 1.6g of graphite powder and 0.8g of N-hexyl pyridine hexafluorophosphate (HPPF6) into a mortar, grinding for 3h to obtain carbon paste, filling the carbon paste into a glass electrode tube with the inner diameter of 4mm, compacting, and inserting a copper wire as a lead to obtain the carbon ion liquid electrode CILE.
(2) Modifying ZIF-8: and (3) dripping 8 mu L of 0.2mg/mL ZIF-8 suspension on the surface of the CILE obtained in the step (2) to obtain the ZIF-8 modified CILE, namely ZIF-8/CILE.
(3) Electrodeposition of Cl2-MWNTs: soaking the ZIF-8/CILE electrode obtained in the step (2) in 1mg/mL Cl2in-MWNTs solution, cyclic voltammetry is adopted, initial voltage and high potential are set to be 0.5V, low potential is-1.5V, and Cl is electrodeposited in an interval in a cyclic scanning mode2MWNTs are arranged on the surface of the ZIF-8/CILE electrode, the number of scanning turns is 10, and the whole electrodeposition process is carried out in a nitrogen atmosphere. Washing with ultrapure water, standing and drying to obtain Cl2-MWNTs/ZIF-8/CILE；
(4) Modified monoclonal antibodies: cl in said step (3)2-MDropwise coating 12 mu L of 15mg/mL monoclonal antibody solution on the surface of the WNTs/ZIF-8/CILE electrode to obtain monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE；
(5) Covering with a Nafion film: the monoclonal antibody/Cl obtained in said step (4)260 mu L of 0.5% Nafion ethanol solution is dripped on the surface of the MWNTs/ZIF-8/CILE electrode, so that the electrode material can be stably fixed on the electrode, and the preparation of the nano electrochemical biosensor is finished, namely Nafion/monoclonal antibody/Cl2MWNTs/ZIF-8/CILE electrode.
Application example
The electrochemical biosensor prepared in example 1 for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether (TBBPA DHEE) comprises the following steps:
the Nafion/monoclonal antibody/Cl prepared in example 1 was used2-MWNTs/ZIF-8/CILE is used as a working electrode, an Ag/AgCl electrode is used as a reference electrode, a platinum sheet is used as an auxiliary electrode, a three-electrode system is established, a Tris-HCl buffer solution with the pH value of 8.0 is used as a supporting electrolyte, and a cyclic voltammetry curve is recorded; and calculating the concentration of TBBPA DHEE in the solution to be detected according to the concentration of TBBPA DHEE and the current change.
The relation between the TBBPA DHEE concentration and the current change is used for calculating that the detection range of the TBBPA DHEE is 0.6 ng/mL-4.5 ng/mL, and the detection limit is 0.25 ng/mL.
The above examples are only preferred embodiments of the present invention, and the scope of the present invention is not limited to the above examples, and all technical solutions belonging to the idea of the present invention belong to the scope of the present invention. It should be noted that improvements and modifications within the scope of the present invention, which do not depart from the principle of the present invention, should be considered within the scope of the present invention by those skilled in the art.
Claims (8)
1. An electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether is characterized in that a carbon ion liquid electrode is taken as a substrate electrode, and a zinc metal-organic framework nano material layer, a chlorine-doped multi-wall carbon nano tube layer, a monoclonal antibody layer and a Nafion film are sequentially modified on the surface of the substrate electrode.
2. The method for preparing an electrochemical biosensor as set forth in claim 1, comprising the steps of:
s1, modifying ZIF-8: dripping 0.2-0.6 mg/mL ZIF-8 suspension on the surface of the carbon ion liquid electrode to obtain a ZIF-8 modified CILE;
s2, electrodepositing Cl2-MWNTs: electrodepositing the chlorine-doped multi-walled carbon nanotube on the surface of the electrode obtained in the step S1 to obtain Cl2-MWNTs/ZIF-8/CILE；
S3, modifying the monoclonal antibody: dripping 12-18 mg/mL monoclonal antibody solution on the surface of the electrode obtained in the step S2 to obtain monoclonal antibody/Cl2-MWNTs/ZIF-8/CILE；
S4, covering a Nafion membrane: and (4) dripping Nafion ethanol solution on the surface of the electrode obtained in the step S3 to ensure that all electrode materials can be stably fixed on the surface of the electrode, thus obtaining the nano electrochemical biosensor.
3. The method according to claim 2, wherein in step S2, cyclic voltammetry is used, initial voltage and high potential are set to 0.5V, low potential is-1.5V, and electrodeposition of Cl is cyclically scanned in an interval2MWNTs, number of scanning turns 10 turns, and the whole electrodeposition process is in nitrogen atmosphere.
4. The preparation method of claim 2, wherein the modified ZIF-8 of step S1 is obtained by dropping 4-8 μ L of 0.2-0.6 mg/mL ZIF-8 suspension on the surface of CILE.
5. The method of claim 2, wherein the modified polyclonal antibody of step S3 is Cl obtained in step S22And 8-12 mu L of 15mg/mL monoclonal antibody solution is dripped on the surface of the MWNTs/ZIF-8/CILE electrode.
6. The method of claim 2, wherein step S4 is the monoclonal antibody/Cl obtained in step S3260-100 mu L of 0.5% Nafion is dripped on the surface of-MWNTs/ZIF-8/CILEEthanol solution.
7. Use of the electrochemical biosensor of claim 1 for detecting tetrabromobisphenol a bis (2-hydroxyethyl) ether.
8. The use according to claim 4, wherein the electrochemical biosensor of claim 1 is used as a working electrode, a platinum sheet electrode as a counter electrode, an Ag/AgCl electrode as a reference electrode, and Tris-HCl buffer solution with pH of 8 as a supporting electrolyte, a three-electrode system is established, and the concentration of tetrabromobisphenol A bis (2-hydroxyethyl) ether in the sample is calculated from the relationship between the current and the concentration of tetrabromobisphenol A bis (2-hydroxyethyl) ether by cyclic voltammetry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910817348.2A CN110672688B (en) | 2019-08-30 | 2019-08-30 | Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910817348.2A CN110672688B (en) | 2019-08-30 | 2019-08-30 | Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110672688A true CN110672688A (en) | 2020-01-10 |
| CN110672688B CN110672688B (en) | 2021-11-12 |
Family
ID=69076091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910817348.2A Active CN110672688B (en) | 2019-08-30 | 2019-08-30 | Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110672688B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022049540A1 (en) * | 2020-09-03 | 2022-03-10 | Gwanzura Zvikomborero Takunda | An electrochemical biosensor |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103941008A (en) * | 2014-04-10 | 2014-07-23 | 山东理工大学 | Method for manufacturing immunosensor for detecting chlorpyrifos |
| CN105784822A (en) * | 2016-04-19 | 2016-07-20 | 青岛科技大学 | Preparation and application methods for electrochemical DNA sensor based on chitosan-graphene/ gold nanoparticle composite membrane |
| CN106053572A (en) * | 2016-07-05 | 2016-10-26 | 济南大学 | Method for preparing electrochemical biphenol A sensors |
| CN106841639A (en) * | 2017-01-06 | 2017-06-13 | 南京师范大学 | A kind of method that female phenol and bisphenol-A are detected based on double antibody sandwich method |
| CN108132290A (en) * | 2018-01-31 | 2018-06-08 | 青岛科技大学 | A kind of electrochemica biological sensor and its preparation and application based on gold nano triangular plate and horseradish peroxidase modified electrode |
| CN108519416A (en) * | 2018-04-08 | 2018-09-11 | 海南师范大学 | A kind of preparation method of the electrochemical sensor based on gold nano chain and myoglobins modified electrode is applied with analysis |
| CN108982626A (en) * | 2018-05-30 | 2018-12-11 | 海南师范大学 | It is a kind of for detecting the nano electrochemical enzyme sensor and its preparation method and application of trichloroacetic acid or sodium nitrite |
-
2019
- 2019-08-30 CN CN201910817348.2A patent/CN110672688B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103941008A (en) * | 2014-04-10 | 2014-07-23 | 山东理工大学 | Method for manufacturing immunosensor for detecting chlorpyrifos |
| CN105784822A (en) * | 2016-04-19 | 2016-07-20 | 青岛科技大学 | Preparation and application methods for electrochemical DNA sensor based on chitosan-graphene/ gold nanoparticle composite membrane |
| CN106053572A (en) * | 2016-07-05 | 2016-10-26 | 济南大学 | Method for preparing electrochemical biphenol A sensors |
| CN106841639A (en) * | 2017-01-06 | 2017-06-13 | 南京师范大学 | A kind of method that female phenol and bisphenol-A are detected based on double antibody sandwich method |
| CN108132290A (en) * | 2018-01-31 | 2018-06-08 | 青岛科技大学 | A kind of electrochemica biological sensor and its preparation and application based on gold nano triangular plate and horseradish peroxidase modified electrode |
| CN108519416A (en) * | 2018-04-08 | 2018-09-11 | 海南师范大学 | A kind of preparation method of the electrochemical sensor based on gold nano chain and myoglobins modified electrode is applied with analysis |
| CN108982626A (en) * | 2018-05-30 | 2018-12-11 | 海南师范大学 | It is a kind of for detecting the nano electrochemical enzyme sensor and its preparation method and application of trichloroacetic acid or sodium nitrite |
Non-Patent Citations (3)
| Title |
|---|
| JUAN LIU ET AL: "Construction of Nafion/Hb/Au/ZIF-8/CILE and its application as electrochemical sensor for determination of bromate and nitrite", 《 INT. J. ELECTROCHEM. SCI.》 * |
| 王韬懿 等: "基于聚锂均苯三甲酸/多壁碳纳米管复合膜的葡萄糖生物传感器的研究", 《分析化学》 * |
| 高建生 等: "氯掺杂提高多壁碳纳米管的电导率", 《化学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022049540A1 (en) * | 2020-09-03 | 2022-03-10 | Gwanzura Zvikomborero Takunda | An electrochemical biosensor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110672688B (en) | 2021-11-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Qiu et al. | An electrochemical ratiometric sensor based on 2D MOF nanosheet/Au/polyxanthurenic acid composite for detection of dopamine | |
| Song et al. | Ratiometric electrochemical glucose biosensor based on GOD/AuNPs/Cu-BTC MOFs/macroporous carbon integrated electrode | |
| Qiu et al. | A label-free amperometric immunosensor based on biocompatible conductive redox chitosan-ferrocene/gold nanoparticles matrix | |
| US10871463B2 (en) | Preparation method based on simultaneous package of target substance and synthesis of MOFs with redox activity | |
| CN110412096B (en) | Preparation method and detection application of reduced graphene oxide @ ZIF-8 composite membrane modified electrode | |
| CN101526493B (en) | Electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof | |
| Feng et al. | A label-free electrochemical immunosensor for rapid detection of salmonella in milk by using CoFe-MOFs-graphene modified electrode | |
| CN101832965B (en) | Laccase biosensor based on magnetic carbon nano tube and chitosan/silicon dioxide gel and preparation method and application thereof | |
| CN109490385B (en) | Biosensor based on Au-ZIF-8/OMC mesoporous carbon | |
| CN108982626B (en) | Nano electrochemical enzyme sensor for detecting trichloroacetic acid or sodium nitrite and preparation method and application thereof | |
| Feng et al. | Nanoporous PtCo-based ultrasensitive enzyme-free immunosensor for zeranoldetection | |
| Gong et al. | Microperoxidase-11/metal–organic framework/macroporous carbon for detecting hydrogen peroxide | |
| Liang et al. | Fabrication, characterization, and application of potentiometric immunosensor based on biocompatible and controllable three-dimensional porous chitosan membranes | |
| CN109085222B (en) | Ionic liquid functionalized graphene vibrio DNA electrochemical sensor and preparation method and application thereof | |
| Guo et al. | Electrochemical behavior of MOF-801/MWCNT-COOH/AuNPs: A highly selective electrochemical sensor for determination of guanine and adenine | |
| Zhu et al. | Rapid fabrication of electrode for the detection of alpha fetoprotein based on MnO2 functionalized mesoporous carbon hollow sphere | |
| Xu et al. | An ultra-sensitive dual-signal ratiometric electrochemical aptasensor based on functionalized MOFs for detection of HER2 | |
| CN110672688B (en) | Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether and preparation method and application thereof | |
| CN112986359B (en) | Based on CuBTC @ MoS 2 -AuNPs modified electrode and CA125 detection method | |
| CN110702759B (en) | ZIF-8 composite material electrochemical immunosensor for detecting alpha fetoprotein and preparation method and application thereof | |
| CN109490282B (en) | Based on NiFe2O4Nano-tube catalysis enhanced ovarian cancer marker ratio type electrochemiluminescence sensing platform | |
| CN105866221B (en) | The electrochemical sensor of reduced hemoglobin can be catalyzed | |
| CN111537584A (en) | Methylene blue-nanoflower, electrochemical aptamer biosensor system, and preparation method and application thereof | |
| CN113484390B (en) | Tumor marker rapid high-sensitivity detection method based on microelectrode | |
| CN110618186B (en) | Preparation method of WO3-CNTs hybrid material and application of hybrid material in tetracycline sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |