CN108519416A - A kind of preparation method of the electrochemical sensor based on gold nano chain and myoglobins modified electrode is applied with analysis - Google Patents
A kind of preparation method of the electrochemical sensor based on gold nano chain and myoglobins modified electrode is applied with analysis Download PDFInfo
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- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
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- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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Abstract
The present invention is prepared for a kind of modified electrode based on gold nano chain (AuNC) and myoglobins (Mb), and is used as Electrochemical enzyme biosensor detection trichloroacetic acid (TCA) and sodium nitrite (NaNO2), show highly sensitive, broad linear range, the preparation method of the gold nano chain and myoglobins modified electrode includes the following steps：（1）According to mass ratio（1.5~2.5）：1 takes graphite powder and ionic liquid N hexyl pyridine hexafluorophosphate (HPPF6) in mortar grind uniformly obtain carbon paste filling glass tube in be compacted, interpolation copper wire obtains carbon ion liquid electrode (CILE) as conducting wire；（2）It takes appropriate AuNC solution drop coating on CILE basal electrodes surface, is protected from light naturally dry under room temperature, obtain AuNC/CILE electrodes；（3）It takes appropriate Mb solution drop coating in AuNC/CILE electrode surfaces, is protected from light dries at room temperature, obtain Mb/AuNC/CILE electrodes；（4）It takes appropriate Nafion ethanol solutions drop coating in Mb/AuNC/CILE electrode surfaces, is protected from light dries to get Nafion/Mb/AuNC/CILE electrodes at room temperature；（5）Trichloroacetic acid and sodium nitrite are detected, preferable effect is shown.
Description
Technical field
The present invention relates to bioelectrochemistry and electrochemical sensing technical fields, more particularly to one kind being based on gold nano chain and flesh
The preparation method of the electrochemical sensor of Lactoferrin modified electrode is applied with analysis.
Background technology
Electrochemica biological sensor be it is a kind of by the biological components such as enzyme, antibody introduce electrode interface, by these biomaterials
As sensing element, various working electrodes such as solid electrode, ion selective electrode as conversion element, record current, conductance,
The electrochemical characteristics such as current potential, resistance or capacitance detect the sensor of signal.Since biosensor is with single-minded, quick, sensitive
The advantages that, it is widely used to the fields such as clinical detection, biochemical analysis and environmental monitoring.Wherein mediated enzyme biosensors are research
A most types, it is using enzyme or the biomolecule with enzymatic structure is sensing element, is that detection is believed with current signal
Number a kind of common enzyme sensor.With the continuous rapid development of sensing technology, the type of biosensor is also more and more,
Electrochemica biological sensor wherein especially based on immobilised enzymes is with the fastest developing speed.Bioelectrochemical sensor about enzyme
Development experience three generations, the first generation be glucolase sensor, by detecting the consumption of oxygen in solution to measure grape
Sugared concentration；The second generation is to be fixed on electrode surface system together as electron mediator and enzyme using micromolecular compounds such as ferrocene
At biosensor；The third generation is that bioactive substance is affixed directly to electrode surface, is made in the redox active of enzyme
The heart and electrode are in direct contact, the more rapid direct electron transfer realized between biomolecule and electrode.Study protein and enzyme
Direct Electrochemistry behavior will establish important theoretical foundation for the exploitation of Electrochemical enzyme biosensor of new generation.
Myoglobins (myoglobin, Mb) is a kind of water-solubility protein, and relative molecular mass 17800 is primarily present
In the myocyte of muscle, it is a kind of containing there are one the single chain protein matter of heme group, the empty the 6th is axial
Position plays a part of to store up oxygen and oxygen carrier in the cell, in addition can also be with H2O、CO、OH-、F-、H2S、CN-、NO2-, NO plasmas or point
Son coordination or bonding.Because its can as cardiomyopathies diagnose foundation and structure it is relatively simple, the Direct Electrochemistry about Mb
Research can be to explore electron mechanism based theoretical in life process.
Gold nano-material is one of metal nano material of most study, it has unique physics and chemical property, by
Gradually become the hot spot of scientific research.First, gold nano-material can be prepared by simple mode, and its property is very steady
Fixed, preparation, which finishes and preserves physics and chemical property in the time that good gold nano-material can be maintained at the several months, not to be occurred
Variation.Gold nano chain (AuNC) is a kind of novel nanogold material synthesized in recent years, it has chain structure, by nanogold
What particle was together in series, the active change of bioprotein will not be caused, it can possess after the corresponding trim of surface modification
Preferable biocompatibility.The surfaces AuNC are easy to, by the moiety combinations such as covalent effect and amino, sulfydryl, be easy to be modified with identification
The coupling of the large biological molecules such as protein, nucleic acid, the enzyme of ability becomes bioprobe, for detecting corresponding target substance, cashes
It is strong to go out specificity, Objective, detects the features such as limit is low, and the range of linearity is wider.
Trichloroacetic acid（TCA）It is CCl4The by-product of metabolism, it is readily formed organic halogen environment in the environment
Pollutant, and be proved to have " three cause " Nie tooth class animals（Carcinogenic, aberration inducing, mutagenesis）Effect.But it is wide
It is general to be applied to agricultural and public safety field, it is such as frequently present in the drinking water sterilized with hypochlorous acid.The Ministry of Construction of China and
What the Ministry of Public Health formulated《National urban water supply water standard》Provide the concentration of dichloroacetic acid and trichloroacetic acid and no more than 0.06
mg·L-1, the security presence amount of trichloroacetic acid is 100 μ gL in World Health Organization's clear stipulaties water environment-1。
Nitrite generally existing in food, drinking water and environment, is mainly derived from feces of livestock and poultry, debirs, change
The nitrite for learning the itrogenous organic substances and nitrate bioconversion such as fertilizer, crude sedimentation, is a kind of inorganic pollution, to the mankind
Health has prodigious harm.Nitrite can act on protein and generate high carcinogenic nitrosamine, Excess free enthalpy nitrite
Many health problems, such as gastric cancer and cancer of the esophagus, baby's methemoglobinemia can be caused.Many countries and tissue all define
The maximum Limited Doses of environment nitrite, China's national Specification nitrite can be used as color stabilizer and preservative, salting down
Maximum usage amount is 0.15gkg in the food such as bacon, dry fruit beetle-1。
To sum up, a kind of model electrochemical enzyme sensor is researched and developed, it is fast to trichloroacetic acid and both substances of nitrite energy
Speed and accurately detection are particularly important.
Invention content
In view of this, being sensed based on the electrochemical enzymatic of gold nano chain and myoglobins modified electrode the present invention provides a kind of
Device preparation method and its analysis application, obtained modified electrode is to trichloroacetic acid and both substances of sodium nitrite with good
Electro-catalysis reduction effect, the advantages that showing the wide range of linearity, detection limit low, high sensitivity, both can be applied to very well
The assay of substance.
The present invention provides a kind of electrochemical sensor based on gold nano chain and myoglobins modified electrode, it is characterised in that
The electrode includes basal electrode, gold nano chain（AuNC）Coating, myoglobins（Mb）Coating, Nafion coatings, the substrate electricity
Extremely Ionic Liquid Modified carbon paste electrode（CILE）.
The present invention provides a kind of electrochemical sensor, and electrode composition is followed successively by CILE basal electrodes, AuNC from inside to outside
The electrode of coating, Mb coatings, Nafion coatings, i.e. Nafion/Mb/AuNC/CILE electrodes.
Another embodiment of the present invention provides above-mentioned gold nano chain and myoglobins modified electrode（It is preferred that Nafion/Mb/
AuNC/CILE electrodes）Preparation method, it is characterised in that include the following steps：
（1）It takes appropriate AuNC solution drop coating on CILE basal electrodes surface, is protected from light naturally dry at room temperature, obtains AuNC/CILE
Electrode；
（2）It takes appropriate Mb solution drop coating in AuNC/CILE electrode surfaces, is protected from light naturally dry at room temperature, obtains Mb/AuNC/
CILE electrodes；
（3）Take appropriate Nafion ethanol solutions drop coating in Mb/AuNC/CILE electrode surfaces, be protected from light at room temperature after drying to get
To Nafion/Mb/AuNC/CILE electrodes；
Step（1）The preparation method of the CILE basal electrodes includes the following steps：By suitable graphite powder and ionic liquid
HPPF6It is placed in grinding in mortar and uniformly obtains carbon paste, then carbon paste is inserted in vitreous electricity pole pipe and is compacted, interpolation copper wire is used as and leads
Line obtains Ionic Liquid Modified carbon paste electrode, i.e. basal electrode CILE；Wherein, graphite powder and ionic liquid HPPF6Mass ratio
It is 1.5 ~ 2.5:1, preferably 2:1；The time of grinding is 2.5 ~ 5.0 h；Glass electrode bore is 4 mm；
Step（1）Described in AuNC solution a concentration of 2.33 nmol L-1, dosage can even spread electrode table with 6 ~ 8 μ L
Face is advisable；
Step（2）A concentration of 10 ~ 20 mg mL of the Mb solution-1, preferably 15 mg mL-1, dosage can be uniformly with 4 ~ 8 μ L
Coating AuNC/CILE electrode surfaces are advisable；
Step（3）The volumetric concentration of the Nafion ethanol solutions is 0.3 ~ 0.7%, preferably 0.5%, and dosage can be with 6 ~ 8 μ L
Even coating Mb/AuNC/CILE electrode surfaces are advisable.
Another embodiment of the present invention provides above-mentioned gold nano chain and myoglobins modified electrode（It is preferred that Nafion/Mb/
AuNC/CILE electrodes）Electrochemical behavior research.
Another embodiment of the present invention provides above-mentioned gold nano chain and myoglobins modified electrode（It is preferred that Nafion/Mb/
AuNC/CILE electrodes）Application in electro-catalysis restores trichloroacetic acid.
Another embodiment of the present invention provides above-mentioned gold nano chain and myoglobins modified electrode（It is preferred that Nafion/Mb/
AuNC/CILE electrodes）Research in Electrochemical Detection trichloroacetic acid.
Another embodiment of the present invention provides above-mentioned gold nano chain and myoglobins modified electrode（It is preferred that Nafion/Mb/
AuNC/CILE electrodes）Application in electro-catalysis restores sodium nitrite.
Another embodiment of the present invention provides above-mentioned gold nano chain and myoglobins modified electrode sensor（It is preferred that
Nafion/Mb/AuNC/CILE electrodes）Research in Electrochemical Detection sodium nitrite.
The detection environment of gold nano chain and myoglobins modified electrode of the present invention is molten in the PBS of pH 2.0 bufferings
In liquid.
The above-mentioned preparation method step of the present invention（1）-（3）The dosage of middle AuNC solution, Mb solution, Nafion ethanol solutions,
It is advisable with energy even spread electrode surface, generally 5 ~ 10 μ L, those skilled in the art can be according to electrode and coating solution
Property, reasonably select the dosage of AuNC solution, Mb solution, Nafion ethanol solutions.
In the present invention, CILE is Ionic Liquid Modified carbon paste electrode, ionic liquid HPPF6For N- hexyl pyridine hexafluorophosphoric acids
Salt, AuNC are gold nano chain, and Mb is myoglobins, and Nafion is perfluorinated sulfonic acid-teflon-copolymers, and TCA is three chloroethenes
Acid, NaNO2For sodium nitrite, PBS buffer solution is phosphate buffer solution；The solution being related to（Except having indicated）It is water-soluble
Liquid.
Compared with the prior art, the advantages of the present invention are as follows：（1）A kind of third generation electrification is prepared using gold nano chain
Enzyme sensor part (Nafion/Mb/AuNC/CILE) is learned, which not only maintains the biological conformation of Mb, cyclic voltammetry scan
Show that a pair of symmetrical redox peaks, the addition of the AuNC of high conductivity make interface impedance value become smaller.（2）Nafion/
Mb/AuNC/CILE electrodes show good electro-catalysis reducing property to trichloroacetic acid and sodium nitrite, can effectively amplify electric current
Signal, the detection range of linearity is wide, and detection limits low, high sensitivity.
Description of the drawings
Fig. 1 is the scanning electron microscopic picture of AuNC/CILE prepared by example 1.
Fig. 2 is PBS buffer solution of the different modifying electrode in pH 2.0, and sweep speed is 100 mV s-1When cycle volt
Antu, wherein curve d are the cyclic voltammetry curve of Nafion/Mb/AuNC/CILE, and curve c is the Nafion/Mb/ of comparative example 1
The cyclic voltammetry curve of CILE, curve b are the cyclic voltammetry curve of the Nafion/AuNC/CILE of comparative example 2, and curve a is comparison
The cyclic voltammetry curve of the Nafion/CILE of example 3.
Fig. 3 is cyclic voltammograms of the Nafion/Mb/AuNC/CILE under different scanning speed, and speed is swept from a to j respectively
For 100,200,300,400,500,600,700,800,900,1000 mV/s.
Fig. 4 is cyclic voltammogram (a to the f of Nafion/Mb/AuNC/CILE in different pH buffer solutions： 2.0,3.0,
4.0, 5.0, 6.0, 7.0 )。
Fig. 5 is cyclic voltammograms of Nafion/Mb/AuNC/CILE in the presence of various concentration TCA, and a to j is 0,40,
80, 120, 160, 200, 250, 300, 350, 400mmol L-1, wherein illustration is the concentration of reduction peak current and TCA
Between graph of relation.
Fig. 6 is Nafion/Mb/AuNC/CILE in various concentration NaNO2In the presence of cyclic voltammogram, a to i be 0,
0.28, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.8 mmol L-1, wherein illustration is reduction peak current and NaNO2's
Graph of relation between concentration.
Specific implementation mode
For the ease of a further understanding of the present invention, examples provided below has done more detailed description to it.But
It is that these embodiments are only not supposed to be a limitation to the present invention or implementation principle for being better understood from invention, reality of the invention
The mode of applying is not limited to the following contents.
Embodiment 1
（1）Take 1.6 g graphite powders and 0.8 g ionic liquids HPPF6It is placed in mortar and grinds 2.5 h, obtain carbon paste, then by carbon
It is compacted in the vitreous electricity pole pipe that paste filling internal diameter is 4 mm, interpolation copper wire obtains CILE as conducting wire；
（2）Take appropriate a concentration of 2.33 nmol L-1AuNC solution（4-8 μL）Drop coating is kept away under room temperature on the surfaces CILE
Light naturally dry obtains AuNC/CILE electrodes；
（3）Appropriate a concentration of 15 mg mL are taken again-1Mb solution（6-8 μL）Drop coating is in AuNC/CILE electrode surfaces, in room temperature
Under be protected from light naturally dry, obtain Mb/AuNC/CILE electrodes；
（4）Finally take appropriate 0.5% Nafion ethanol solutions（6-8 μL）Drop coating is in Mb/AuNC/CILE electrode surfaces, at room temperature
It is protected from light after drying to get to Nafion/Mb/AuNC/CILE electrodes.
Comparative example 1
（1）Take 1.6 g graphite powders and 0.8 g ionic liquids HPPF6It is placed in mortar and grinds 2.5 h, obtain carbon paste, then by carbon
It is compacted in the vitreous electricity pole pipe that paste filling internal diameter is 4 mm, interpolation copper wire obtains CILE as conducting wire；
（2）Take appropriate a concentration of 15 mg mL-1Mb solution（6-8 μL）Drop coating is protected from light at room temperature in CILE electrode surfaces
Naturally dry obtains Mb/CILE electrodes；
（3）Finally take appropriate 0.5% Nafion ethanol solutions（6-8 μL）Drop coating is protected from light at room temperature in Mb/CILE electrode surfaces
To get to Nafion/Mb/CILE electrodes after drying.
Comparative example 2
（1）Take 1.6 g graphite powders and 0.8 g ionic liquids HPPF6It is placed in mortar and grinds 2.5 h, obtain carbon paste, then by carbon
It is compacted in the vitreous electricity pole pipe that paste filling internal diameter is 4 mm, interpolation copper wire obtains CILE as conducting wire；
（2）Take appropriate a concentration of 2.33 nmol L-1AuNC solution（8-10 μL）Drop coating is on the surfaces CILE, under room temperature
It is protected from light naturally dry, obtains AuNC/CILE electrodes；
（3）Finally take appropriate 0.5% Nafion ethanol solutions（6-8 μL）Drop coating is kept away at room temperature in AuNC/CILE electrode surfaces
Light dry after to get to Nafion/AuNC/CILE electrodes.
Comparative example 3
（1）Take 1.6 g graphite powders and 0.8 g ionic liquids HPPF6It is placed in mortar and grinds 2.5 h, obtain carbon paste, then by carbon
It is compacted in the vitreous electricity pole pipe that paste filling internal diameter is 4 mm, interpolation copper wire obtains CILE as conducting wire；
（2）Take appropriate 0.5% Nafion ethanol solutions（6-8 μL）Drop coating is protected from light in CILE electrode surfaces after drying at room temperature,
Obtain Nafion/CILE electrodes.
One, electrochemical AC impedance collection of illustrative plates is studied
Electrochemical alternate impedance spectrum (EIS) can reflect modified electrode its after surface modification interface resistance variation, and electricity
Sub- transfer resistance (Ret) value can be acquired by the diameter of the semi arch of high-frequency region.
The present invention has investigated different modifying electrode in 10 mmol L-1 [Fe(CN)6]3-/4-With 0.1 mol L-1KCl is mixed
Range of scanned frequencies is in 10 in conjunction solution5To the electrochemical AC impedance collection of illustrative plates of 0.1 Hz, and acquire corresponding Ret values result such as
Shown in lower：
It is above-mentioned the experimental results showed that, the impedance value of Nafion/AuNC/CILE is minimum, shows that there is gold nano chain high metal to lead
Electrically interface resistance is made to become smaller；The impedance value of Nafion/Mb/CILE is maximum, shows nonconducting large biological molecule Mb in electrode
The presence on surface hinders the transmission of electronics, increases electron transmission resistance；The Nafion/Mb/AuNC/CILE's of embodiment 1
Impedance value ratio Nafion/Mb/CILE is obviously reduced, and also indicates that the presence of the AuNC of high conductivity can effectively reduce interface
Electron transmission resistance.
Two, the Direct Electrochemistry behavioral study of Mb
Mb is had studied in the PBS buffer solutions of pH=2.0,100 mV s of sweep speed-1When cyclic voltammetry scan to study it straight
Electrochemical behavior is connect, the results are shown in Figure 2, as can be seen from the figure：
In curve (a) Nafion/CILE（Comparative example 3）On, occur without any redox peaks, i.e., surface is not electroactive
Substance is present in electrode surface.
In curve (b) Nafion/AuNC/CILE（Comparative example 2）On, except the feature redox peaks of small gold occur,
An almost smoothed curve occurs without any peak.
In curve (c) Nafion/Mb/CILE（Comparative example 1）On, there are a pair of of redox peaks, peak current is smaller, this
Illustrate that there is electronics transfers between Mb molecules and CILE electrodes.
In curve (d) Nafion/Mb/AuNC/CILE（Embodiment 1）On, there is the oxidation that a pair of of peak shape is good and stablizes
Reduction peak.The presence of AuNC causes peak current to significantly increase, this illustrates electric between the presence of AuNC accelerates Mb and CILE electrodes
Sub- transfer rate.AuNC used in the present invention has excellent electric conductivity, and chain material, which interconnects, has faster electronics
Transmission channels, good biocompatibility can provide big specific surface area and adsorb a large amount of Mb molecules, be conducive to accelerate electronics turn
It moves.
Can be obtained from curve d spike potential be respectively Epa be -0.144V (vsSCE) and Epc be -0.239 V
(vsSCE), spike potential poor (△ Ep) is 0.095V, Middle Eocene E0'For -0.191 V (vsSCE), redox peak current
The ratio between close to 1, show the characteristic electrochemical behavior of prosthetic heme group Fe (III) in protein/Fe (II) electricity pair, illustrate Mb
Direct Electrochemistry behavior be achieved and be accelerated.
Three, influence of the research sweep speed to Mb electrochemical responses
The influence of Nafion/Mb/AuNC/CILE electrochemical signals response of the sweep speed to the embodiment of the present invention 1 is had studied,
The results are shown in Figure 3.In 100 ~ 1000 mV s-1Sweep speed within the scope of obtained the symmetrical redox of a pair of Mb
Peak, and redox peak current (Ipa and Ipc) increases with the raising of sweep speed, shows that the Direct Electrochemistry of Mb is one
Electrode process that will definitely be inverse.Equation of linear regression is respectively Ipa (μ A)=- 464.8 υ (Vs-1)-26.53(n=20,γ=
And Ipc (μ A)=536.7 υ (Vs 0.996)-1)+40.22(n=20, γ=0.996).Show on Nafion/Mb/AuNC/CILE
Reaction belong to thin layer electrochemistry behavior, i.e. electrode reaction is controlled by adsorption process.By formula Q=nAF Γ *（Q is the quantity of electric charge, n
For the electronics transfer number in electrode reaction, A is the area of electrode, and F is Faraday constant, and Γ * are known as electroactive material in electricity
The surface coverage Γ * of pole）, it can be deduced that Γ * values are 3.0 × 10-9 mol•cm-2。
With the increase of sweep speed, spike potential difference △ Ep gradually increase, and spike potential is distinguished with ln υ equations of linear regression
For Epc (V)=- 0.041ln υ (Vs-1) -0.29 (n=8, γ=0.98) and Epa (V)=0.036ln υ (Vs-1)-0.064(n
=8, γ=0.99).According to the theoretical corresponding formula of Laviron（1-3）：
It is 1.16 that electron transfer number, which can be calculated, electron transmission coefficientαIt is 0.47.Reaction rate constantk s For 0.76 s-1, say
The Direct electron transfer of Mb has faster rate in bright AuNC modified electrodes.
Four, influences of the research pH to Mb electrochemical signals
Five, to the research of TCA electro-catalysis behaviors
IssIt is steady-state current when forming good linear relationship after adding substrate, C is the concentration that substrate is added in 10 mL PBS,
ImaxBe TCA concentration be more than 400.0 mmol L-1When the saturation of substrates state that is formed under measured maximum current.It can root
According to double-reciprocal plot method (1/Iss~ 1/ [TCA]) calculate catalysis reactions of the Nafion/Mb/AuNC/CILE to TCAK M app For
5.7 mmol L-1, show that Nafion/Mb/AuNC/CILE is good to the catalytic effect of TCA.
Six, to NaNO2The research of electro-catalysis behavior
The Nafion/Mb/AuNC/CILE electro-catalysis of this experimental study embodiment of the present invention 1 restores NaNO2Catalytic effect, knot
Fruit is as shown in Figure 6.NaNO is added in the PBS buffer solutions of pH 2.02After record cyclic voltammetry curve, will appear in -0.588V
One new reduction peak, reduction peak current is with NaNO2The increase of concentration and increase, work as NaNO2Concentration 0.04 ~ 1.8
mmol L-1In the range of when, reduction peak current and NaNO2Concentration at good linear relationship, detection is limited to 0.05
mmol L-1.Work as NaNO2Concentration be more than 1.8mmol L-1When peak current will appear a platform and current value remains unchanged.It can
According to double-reciprocal plot method (1/Iss~1/[NaNO2]) Nafion/Mb/AuNC/CILE is calculated to NaNO2Catalysis reactionK M app For 0.76 mmol L-1。
Claims (9)
1. a kind of modified electrode based on gold nano chain and myoglobins, it is characterised in that the electrode includes basal electrode, Jenner
Rice chain（AuNC）Coating, myoglobins（Mb）Coating, Nafion coatings, the basal electrode are Ionic Liquid Modified carbon paste electrode
（CILE）.
2. a kind of working electrode of Electrochemical enzyme biosensor, it is characterised in that be followed successively by CILE basal electrodes, AuNC from inside to outside
The electrode of coating, Mb coatings, Nafion coatings, i.e. Nafion/Mb/AuNC/CILE electrodes.
3. a kind of electrochemica biological sensor part, it is characterised in that using claim 1-2 any one of them electrode as work
Electrode.
4. claim 1-2 any one of them electrode trichloroacetic acid and sodium nitrite in preparing electrochemica biological sensor part
The application of detection.
5. the preparation method of claim 1-2 any one of them working electrodes, it is characterised in that include the following steps：
（1）It takes appropriate AuNC solution drop coating on CILE basal electrodes surface, is protected from light naturally dry at room temperature, obtains AuNC/CILE
Electrode；
（2）It takes appropriate Mb solution drop coating in AuNC/CILE electrode surfaces, is protected from light naturally dry at room temperature, obtains Mb/AuNC/
CILE electrodes；
（3）Take appropriate Nafion ethanol solutions drop coating in Mb/AuNC/CILE electrode surfaces, be protected from light at room temperature after drying to get
To Nafion/Mb/AuNC/CILE electrodes.
6. the method described in claim 5, it is characterised in that step（1）Described in AuNC solution a concentration of 2.33 nmol L-1；Step（2）A concentration of 10 ~ 20 mg mL of the Mb solution-1, preferably 15 mg mL-1；Step（3）The Nafion ethyl alcohol
The volumetric concentration of solution is 0.3 ~ 0.7%, preferably 0.5%.
7. claim 5-6 any one of them methods, it is characterised in that step（1）The preparation method of the CILE basal electrodes
Include the following steps：By suitable graphite powder and ionic liquid N-hexyl pyridine hexafluorophosphate (HPPF6) be placed in mortar and grind
Mill is uniform, obtains carbon paste, then inserts in vitreous electricity pole pipe carbon paste and is compacted, interpolation copper wire obtains ionic liquid as conducting wire
Decorating carbon paste electrode, i.e. CILE basal electrodes；Wherein graphite powder and ionic liquid HPPF6Mass ratio be 1.5 ~ 2.5:1, preferably
2:1；The time of grinding is 2.5 ~ 5.0 h；Glass electrode bore is 4 mm.
8. application of the claim 1-2 any one of them electrode in electro-catalysis restores trichloroacetic acid or sodium nitrite.
9. application of the claim 1-2 any one of them electrode in detection trichloroacetic acid or sodium nitrite.
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| CN115372430A (en) * | 2022-07-26 | 2022-11-22 | 湖南海源医疗科技股份有限公司 | Carbon slurry for electrochemical test strip, preparation method thereof and electrochemical test strip |
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Application publication date: 20180911 |