CN110672598A - Dry chemical reagent strip for detecting calcium, magnesium and zinc in breast milk - Google Patents
Dry chemical reagent strip for detecting calcium, magnesium and zinc in breast milk Download PDFInfo
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- CN110672598A CN110672598A CN201911015388.1A CN201911015388A CN110672598A CN 110672598 A CN110672598 A CN 110672598A CN 201911015388 A CN201911015388 A CN 201911015388A CN 110672598 A CN110672598 A CN 110672598A
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- dry chemical
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7793—Sensor comprising plural indicators
Abstract
The invention provides a dry chemical reagent strip for simultaneously measuring calcium, magnesium and zinc in breast milk, which specifically comprises a supporting layer and a detection reagent block; the detection reagent block is made of whatman filter paper for adsorbing detection reagents for detecting calcium, magnesium or zinc, and is directly bonded at one end of the strip-shaped supporting layer. When the color test paper is used specifically, the other end of the supporting layer is held by hand, the detection reagent block is completely soaked in a sample (breast milk) to be tested and taken out, and the color test paper is compared with a standard color comparison card after the color is stable. The dry chemical reagent strip provided by the invention can simultaneously detect the contents of calcium, magnesium and zinc, and does not influence each other and pollute a sample. The detection process is efficient and simple, and the result data is reliable.
Description
Technical Field
The invention relates to the technical field of rapid detection of calcium, magnesium and zinc, in particular to a detection reagent for calcium, magnesium and zinc in breast milk and a dry chemical detection reagent strip.
Background
The breast milk is a natural nutriment for babies, and the components of the breast milk directly influence the growth, development, intelligence development and digestive ability of the babies, so the breast milk cannot be replaced by the formula milk. The contents of calcium, magnesium and zinc in the breast milk nutrient components are important for the growth and development of infants. Among them, calcium plays an important role in constituting the bones and teeth of infants, maintaining the normal functions of nerves, blood, glands and heart, etc. Zinc is a trace element necessary for human body, is a composition component of various enzymes of human body, has important physiological functions, participates in protein synthesis in vivo, and promotes cell division, growth and regeneration. Magnesium is an essential element required for cell metabolism, and has important effects on maintaining contractility and neural excitability of muscles, activating enzymes in vivo, and promoting energy metabolism. Calcium deficiency in infants can lead to skeletal changes such as malacia of the skull, enlargement and delayed closure of the anterior chimney, late teething, chicken breast, funnel breast, valgus costalis, deformity of the extremities, flattening of the pelvis, etc. Mild magnesium deficiency can cause unstable emotion, easy excitement, restlessness, and crying of infants. Severe convulsions of hands and feet, hyperreflexia and even convulsions may occur. The deficiency of zinc can lead to bad appetite, thin hair, lusterless color, easy anemia, poor resistance and the like of the infants. Therefore, whether the infant obtains enough nutrients such as calcium, magnesium, zinc and the like from the breast milk can be known in time by detecting the content of the nutrient components in the breast milk, and the infant malnutrition caused by insufficient or unbalanced nutrition of the breast milk can be discovered and corrected as early as possible.
Calcium, magnesium and zinc detection methods are numerous, and the conventional methods for detecting calcium, magnesium and zinc are ultrasonic infrared spectroscopy, liquid biochemical reagent methods and dry chemical reagent methods. The dry chemical reagent method has the characteristics of rapid detection, low cost, simple and flexible operation, few detection samples, accurate detection result, short detection time and the like.
Currently, many products are detected by foreign companies by using a dry chemical reagent method (such as german MN, ekey corporation, and qiangsheng group ltd), which has the disadvantages of complex production process, high requirements on materials and equipment, pretreatment of samples, operation by professionals, and the like.
In addition, US5968833 discloses a dry chemical test paper for measuring magnesium concentration in biological fluid, chinese patent CN98109147.4 discloses a breast milk calcium visual test reagent strip and chinese patent CN20120130424.0 discloses a dry test strip for calcium determination, the methods in these patents are all qualitative or quantitative single detection items, the detection items are single, and multiple items cannot be detected simultaneously. US patent US3511608 discloses a preparation method of a dairy multi-parameter rapid detection card, which has the problems that the test flow is complicated, a test sample needs to be pretreated once during detection, the test can be carried out after pretreatment, and the sample size required by the test is large. At present, no dry chemical reagent strip capable of being used for rapidly detecting calcium ions, magnesium ions and zinc ions in breast milk exists. The invention aims to provide the dry chemical reagent strip which is easy to carry, can simultaneously and quickly detect calcium ions, magnesium ions and zinc ions, is convenient to operate, has accurate detection result, does not need instruments, can detect the contents of calcium, magnesium and zinc by visual colorimetry, and is suitable for large-scale quick screening and household application of breast milk nutritional ingredients.
Disclosure of Invention
The invention provides a dry chemical reagent strip which can simultaneously determine calcium, magnesium and zinc in breast milk, and has the advantages of quick detection, low cost, simple operation, no sample pollution, accurate detection result and short detection time.
A dry chemical reagent strip for simultaneously measuring calcium, magnesium and zinc in breast milk comprises a supporting layer and a detection reagent block; the detection reagent block is a membrane material absorbed with detection reagents; the detection reagent is specifically a detection reagent for detecting calcium, magnesium or zinc.
Wherein, the detection reagent block is adhered on the supporting layer by gluing; the number of the detection reagent blocks bonded on the supporting layer is more than or equal to 1.
Wherein, the membrane material of the detection reaction block is whatman filter paper.
Wherein the supporting layer is made of polystyrene, polyvinyl chloride or polyethylene, and the thickness is 0.2-0.8 mm.
Wherein, the formula of the detection reagent for calcium ions comprises 5-30g of oxalic acid, 0.3-2g of 8-hydroxyquinoline, 0.1-1g of azoarsine-III, 5-20ml of ethanol, 0.5-3g of polyethylene glycol, 5-10ml of glycerol, 0.2-5g of polyvinylpyrrolidone, 0.1-1g of triton, 0.01-3ml of hydrochloric acid and 1000ml of purified water or distilled water.
Wherein, the formula of the detection reagent for the magnesium ions comprises 3 to 20g of citric acid, 0.05 to 4g of xylidine blue, 0.5 to 10ml of methanol, 2 to 8g of mannitol, 1 to 5ml of glycerol, 0.01 to 0.2g of tween, 0.05 to 0.3g of potassium hydroxide and 100 ml of purified water or distilled water.
The formula of the zinc ion detection reagent is 15-40g of 2- (N-morpholine) ethanesulfonic acid, 0.05-3g of 4- (2-pyridylazo) resorcinol sodium salt, 1-10ml of ethanol, 0.3-24g of sucrose, 2-10ml of glycerol, 0.1-4g of triethanolamine, 0.5-3g of sodium citrate, 0.05-0.1g of sodium hydroxide and 200-800ml of purified water or distilled water.
The preparation method of the detection reagent block comprises the following steps: and (3) immersing the membrane material into the detection reagent, immediately taking out the membrane material after immersing for 2-8 minutes, and drying the membrane material in a drying oven at 35-75 ℃.
The method is specifically used for soaking the dry chemical reagent strip into a sample, taking out the sample after 5-10 seconds, and comparing and reading the sample with a standard colorimetric card after the color is stable.
According to the invention, during testing, a dry chemical test strip of calcium, magnesium and zinc is immersed in fresh mother milk which is fully and uniformly mixed, different test paper blocks and reagents in a reaction layer generate color development reaction, the color depth is in direct proportion to the concentration of calcium, magnesium and zinc in a sample, the reaction is carried out for 1 minute and is visually compared with the color of a colorimetric card, and the result is interpreted, so that the content of calcium, magnesium and zinc in the sample can be simultaneously and rapidly detected.
The invention has the beneficial effects that:
1. according to the invention, the designed calcium, magnesium and zinc dry chemical reagent strip is adopted, the detection sample does not need to be pretreated, and the operation is simple and convenient;
2. the reaction of each detection item is pollution-free, the sample is not polluted, the detection time is short, the cost is low, and the method is suitable for large-scale rapid screening and household application;
3. according to the invention, the content of the nutritional ingredients such as calcium, magnesium and zinc in breast milk is measured by using the dry chemical reagent strip, so that whether the infant obtains enough nutritional ingredients such as calcium, magnesium and zinc from the breast milk can be known in time, and the infant malnutrition caused by insufficient or unbalanced nutrition of the breast milk can be discovered and corrected as early as possible.
Drawings
FIG. 1 is a structural diagram of a dry chemical reagent strip for detecting calcium, magnesium and zinc in breast milk.
FIG. 2 is a structural analysis diagram of the dry chemical reagent strip for detecting calcium, magnesium and zinc in breast milk.
Reference numbers in the figures: 1 is a test end, 2 is a handheld end, 3 is a supporting layer, 4 is a reaction layer calcium ion detection reagent block, 5 is a reaction layer magnesium ion detection reagent block, 6 is a reaction layer zinc ion detection reagent block, and 7 is a double-sided adhesive layer.
Detailed Description
The invention is further described below by way of examples.
And (3) judging and reading result method: comparing with a standard colorimetric card by visual inspection, and judging the reading result into the following three grades, wherein the calcium concentration is lower when the calcium concentration is less than 20mg/dl, the calcium concentration is normal when the calcium concentration is 20-50mg/dl, and the calcium concentration is higher when the calcium concentration is more than 50 mg/dl; the magnesium concentration is lower when the magnesium concentration is less than 3mg/dl, the magnesium concentration is normal when the magnesium concentration is 3-7mg/dl, and the magnesium concentration is higher when the magnesium concentration is more than 7 mg/dl; the concentration of zinc is lower when the concentration is less than 400ug/dl, normal when the concentration of zinc is 400 and 800ug/dl, and higher when the concentration of zinc is more than 800 ug/dl.
Examples 1
The supporting layer is made of polyvinyl chloride material, the thickness is 0.21mm, the length is 60mm, and the width is 5 mm.
Preparation of a reaction layer: the reaction layer adopts whatman filter paper as a carrier, the aperture size is 5um, the thickness is 50um, three pieces of whatman filter paper with the length and width of 20 x 20cm are respectively soaked in calcium, magnesium and zinc test paper reagents, the filter paper is immediately taken out and put into a drying oven with the temperature of 60 ℃ for drying after being completely soaked for 2 minutes, the filter paper is taken out after 90 minutes and cut into square small blocks with the size of 5mm x 5mm, the filter paper is aligned and bonded together through double-sided adhesive tape at the interval of 2mm, and the small blocks are put into an aluminum foil bag for sealing.
The formula of the calcium reaction reagent is as follows: 20g of oxalic acid, 1.5g of 8-hydroxyquinoline, 1g of azoarsine-III, 8ml of ethanol, 3g of polyethylene glycol, 8ml of glycerol, 0.5g of polyvinylpyrrolidone, 0.1g of triton, 0.5ml of hydrochloric acid and 600ml of purified water.
The formula of the magnesium reaction reagent is as follows: 15g of citric acid, 2.1g of xylidine blue, 8.2ml of methanol, 6.5g of mannitol, 3ml of glycerol, 0.1g of tween, 0.1g of potassium hydroxide and 200ml of purified water.
The formulation of the zinc reactant is as follows: 15.5g of 2- (N-morpholine) ethanesulfonic acid, 0.55g of 4- (2-pyridylazo) resorcinol sodium salt, 7.5ml of ethanol, 10.5g of sucrose, 4ml of glycerol, 2.5g of triethanolamine, 1.5g of sodium citrate, 0.06g of sodium hydroxide and 500ml of purified water.
Preparing a calcium, magnesium and zinc standard solution: standard solution samples of different concentrations of calcium, magnesium and zinc were prepared as shown in table 1.
TABLE 1
During testing, the calcium, magnesium and zinc dry chemical test strips are respectively immersed in the fully and uniformly mixed samples with different concentrations prepared in the table 1, the samples are taken out after being immersed for 5 to 10 seconds, the samples are visually compared with the color of the colorimetric card for 1 minute, the result is judged, the reaction color of each detection item of the test strips is bright and uniform, the test strips are not polluted, the result can be accurately read, and the result is shown in the table 2.
TABLE 2
Example 2
The supporting layer is made of polyvinyl chloride material, the thickness is 0.4mm, the length is 60mm, and the width is 5 mm.
Preparation of a reaction layer: the reaction layer adopts whatman filter paper as a carrier, the aperture size is 10um, the thickness is 100um, three pieces of whatman filter paper with the length and width of 20cm are respectively soaked in calcium, magnesium and zinc test paper reagents, the whatman filter paper is immediately taken out and put into a drying oven with the temperature of 60 ℃ for drying after being completely soaked for 3 minutes, the whatman filter paper is taken out after 90 minutes and cut into square blocks with the size of 5mm by 5mm, the two sides are aligned and bonded together through double-sided adhesive tape with the interval of 2mm, and the square blocks are put into an aluminum foil bag for sealing.
The formula of the calcium reaction reagent is as follows: 15g of oxalic acid, 1g of 8-hydroxyquinoline, 0.5g of azoarsine-III, 10ml of ethanol, 1.2g of polyethylene glycol, 8ml of glycerol, 2.1g of polyvinylpyrrolidone, 0.2g of triton, 0.05ml of hydrochloric acid and 600ml of purified water.
The formula of the magnesium reaction reagent is as follows: 12g of citric acid, 0.5g of xylidine blue, 5ml of methanol, 3.5g of mannitol, 0.08g of tween, 0.06g of potassium hydroxide, 2ml of glycerol and 150ml of purified water.
The formulation of the zinc reactant is as follows: 30g of 2- (N-morpholine) ethanesulfonic acid, 1.8g of 4- (2-pyridylazo) resorcinol sodium salt, 8ml of ethanol, 20g of sucrose, 5ml of glycerol, 0.2g of triethanolamine, 0.8g of sodium citrate, 0.1g of sodium hydroxide and 700ml of purified water.
Preparing a calcium, magnesium and zinc standard solution: standard solution samples of different concentrations of calcium, magnesium and zinc were prepared as shown in table 3.
TABLE 3
During testing, the calcium, magnesium and zinc dry chemical test strips are respectively immersed in the fully and uniformly mixed samples with different concentrations prepared in the table 3, the samples are taken out after being immersed for 5 to 10 seconds, the samples are visually compared with the color of the colorimetric card for 1 minute, the result is judged, the reaction color of each detection item of the test strips is bright and uniform, the test strips are not polluted, the result can be accurately read, and the result is shown in the table 4.
TABLE 4
Comparative example 1
The formula of the calcium reaction reagent, the formula of the magnesium reaction reagent and the formula of the zinc reaction reagent are not added with the glycerol reagent. Otherwise, the same procedure as in example 1 was repeated.
And (3) respectively soaking the calcium, magnesium and zinc dry chemical test strips into fully and uniformly mixed samples with different concentrations (the same as the example 1), taking out after soaking for 5-10 seconds, reacting for 1 minute, carrying out visual comparison with the color of the color comparison card, and judging the result. The results are shown in Table 5.
TABLE 5
Claims (9)
1. A dry chemical reagent strip for simultaneously measuring calcium, magnesium and zinc in breast milk is characterized by consisting of a supporting layer and a detection reagent block; the detection reagent block is a membrane material absorbed with detection reagents; the detection reagent is specifically a detection reagent for detecting calcium, magnesium or zinc.
2. The dry chemical reagent strip of claim wherein the test reagent block is adhesively bonded to the support layer; the number of the detection reagent blocks bonded on the supporting layer is more than or equal to 1.
3. The dry chemical reagent strip according to claim 1, wherein the membrane material of the detection reaction block is whatman filter paper.
4. The dry chemical reagent strip according to claim 1 wherein the support layer is made of polystyrene, polyvinyl chloride or polyethylene and has a thickness of 0.2-0.8 mm.
5. The dry chemical reagent strip as claimed in claim 1, wherein the formulation of the detection reagent for calcium ions is oxalic acid 5-30g, 8-hydroxyquinoline 0.3-2g, azoarsine-III 0.1-1g, ethanol 5-20ml, polyethylene glycol 0.5-3g, glycerol 5-10ml, polyvinylpyrrolidone 0.2-5g, triton 0.1-1g, hydrochloric acid 0.01-3ml, purified water or distilled water 500-1000 ml.
6. The dry chemical reagent strip as claimed in claim 1, wherein the formulation of the detection reagent for magnesium ions is 3-20g of citric acid, 0.05-4g of xylidine blue, 0.5-10ml of methanol, 2-8g of mannitol, 1-5ml of glycerol, 0.01-0.2g of tween, 0.05-0.3g of potassium hydroxide, and 100 ml of purified or distilled water.
7. The dry chemical reagent strip as claimed in claim 1, wherein the formulation of the detection reagent for zinc ions is 2- (N-morpholine) ethanesulfonic acid 15-40g, 4- (2-pyridylazo) resorcinol sodium salt 0.05-3g, ethanol 1-10ml, sucrose 0.3-24g, glycerin 2-10ml, triethanolamine 0.1-4g, sodium citrate 0.5-3g, sodium hydroxide 0.05-0.1g, and purified or distilled water 200-800 ml.
8. The dry chemical reagent strip according to claim 1, wherein the detection reagent block is prepared by a method comprising: and (3) immersing the membrane material into the detection reagent, immediately taking out the membrane material after immersing for 2-8 minutes, and drying the membrane material in a drying oven at 35-75 ℃.
9. The dry chemical reagent strip according to claim 1, wherein the dry chemical reagent strip is used by immersing the dry chemical reagent strip in a sample for 5 to 10 seconds, taking out the dry chemical reagent strip, and comparing and reading the dry chemical reagent strip with a standard color chart after the color is stabilized.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112285367A (en) * | 2020-12-24 | 2021-01-29 | 中生北控生物科技股份有限公司 | Kit for detecting calcium ions and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040133C (en) * | 1993-08-27 | 1998-10-07 | 联合矿业有限公司 | Method for removing zinc from sulphured concentrates |
| CN1237706A (en) * | 1998-05-30 | 1999-12-08 | 肖佳民 | Reagent strip for visualizing breast milk |
| US20070077168A1 (en) * | 2005-10-05 | 2007-04-05 | Szalczyk Bryarly A | Alcohol-in-breast milk analysis test kit |
| CN101076601A (en) * | 2004-12-13 | 2007-11-21 | 拜尔保健有限公司 | Size self-limiting compositions and test devices for measuring analytes in biological fluids |
| WO2008105764A2 (en) * | 2007-02-28 | 2008-09-04 | King Shannon L | Breast milk contaminant detection device |
| WO2008104696A2 (en) * | 2007-02-19 | 2008-09-04 | L'oreal | Polymer particle dispersion, composition containing the same and cosmetic treatment method |
| CN101710094A (en) * | 2009-10-12 | 2010-05-19 | 张祥成 | Biosensor chip/test strip capable of automatically filtering erythrocytes and leukocytes |
| CN103575815A (en) * | 2012-07-30 | 2014-02-12 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method for detecting glyceryl monostearate in liquid milk |
| CN103884799A (en) * | 2014-04-16 | 2014-06-25 | 光明乳业股份有限公司 | Method for detecting beta-cyclodextrin in dairy product |
| CN104316700A (en) * | 2014-10-11 | 2015-01-28 | 北京华益精点生物技术有限公司 | Preparation method of milk product multi-parameter quick detection card |
-
2019
- 2019-10-24 CN CN201911015388.1A patent/CN110672598B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040133C (en) * | 1993-08-27 | 1998-10-07 | 联合矿业有限公司 | Method for removing zinc from sulphured concentrates |
| CN1237706A (en) * | 1998-05-30 | 1999-12-08 | 肖佳民 | Reagent strip for visualizing breast milk |
| CN101076601A (en) * | 2004-12-13 | 2007-11-21 | 拜尔保健有限公司 | Size self-limiting compositions and test devices for measuring analytes in biological fluids |
| US20070077168A1 (en) * | 2005-10-05 | 2007-04-05 | Szalczyk Bryarly A | Alcohol-in-breast milk analysis test kit |
| WO2008104696A2 (en) * | 2007-02-19 | 2008-09-04 | L'oreal | Polymer particle dispersion, composition containing the same and cosmetic treatment method |
| WO2008105764A2 (en) * | 2007-02-28 | 2008-09-04 | King Shannon L | Breast milk contaminant detection device |
| CN101710094A (en) * | 2009-10-12 | 2010-05-19 | 张祥成 | Biosensor chip/test strip capable of automatically filtering erythrocytes and leukocytes |
| CN103575815A (en) * | 2012-07-30 | 2014-02-12 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method for detecting glyceryl monostearate in liquid milk |
| CN103884799A (en) * | 2014-04-16 | 2014-06-25 | 光明乳业股份有限公司 | Method for detecting beta-cyclodextrin in dairy product |
| CN104316700A (en) * | 2014-10-11 | 2015-01-28 | 北京华益精点生物技术有限公司 | Preparation method of milk product multi-parameter quick detection card |
Non-Patent Citations (2)
| Title |
|---|
| 佚名: "有效锌测定方法", 《百度文库》 * |
| 支丽霞 等: "《临床实用检验医学》", 30 November 2013, 科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112285367A (en) * | 2020-12-24 | 2021-01-29 | 中生北控生物科技股份有限公司 | Kit for detecting calcium ions and application thereof |
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