CN110669150A - Equipment and process for extracting active substances of dendrobium huoshanense - Google Patents
Equipment and process for extracting active substances of dendrobium huoshanense Download PDFInfo
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- CN110669150A CN110669150A CN201911010457.XA CN201911010457A CN110669150A CN 110669150 A CN110669150 A CN 110669150A CN 201911010457 A CN201911010457 A CN 201911010457A CN 110669150 A CN110669150 A CN 110669150A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses dendrobium huoshanense active substance extraction equipment which comprises a heating tank, a primary centrifugal machine, a deproteinizing tank, a secondary centrifugal machine and a chromatography column, wherein an isolation net is installed in the heating tank, a circulation groove is formed in the heating tank, a primary connecting pipe is connected below the heating tank and is connected with the primary centrifugal machine, a primary collecting tank is arranged below the primary centrifugal machine and is connected with the deproteinizing tank, a water bath tank is formed in the deproteinizing tank, a secondary connecting pipe is connected between the primary collecting tank and the deproteinizing tank, and a primary external connecting pipe is arranged at the upper end of the deproteinizing tank. The systematic equipment can well realize the extraction of polysaccharide active substances in the dendrobium huoshanense, the working mode of a production line, the extraction of the active substances is orderly and effectively carried out, and insoluble cellulose such as protein and the like in the dendrobium huoshanense can be removed after multiple times of filtration.
Description
Technical Field
The invention relates to the technical field of active substance extraction, in particular to equipment and a process for extracting active substances of dendrobium huoshanense.
Background
Researches find that the polysaccharide of dendrobium huoshanense has the effects of resisting oxidation, tumors and cataract, improving the immunity of the organism and the like, and the polysaccharide is an active ingredient with higher content in dendrobium huoshanense.
The dendrobium huoshanense is used as a traditional Chinese medicinal material for a long time, but is well known and accepted by people in recent years, the dendrobium huoshanense is researched, is mostly eaten as a medicinal material or food, is eaten by branches and trunks in a use mode, and is directly used by retaining the whole dendrobium huoshanense no matter being dried or directly eaten, but the mode is an indirect use mode for users, needs to depend on consumption of own functions at a little point, and the active ingredients are utilized in a single mode, so that the gastrointestinal absorption of the human body to plants is limited, for example, cellulose in the plants can not be directly digested and absorbed by the human body, so that the absorption of the direct eating to nutrient substances is greatly reduced, and the cellulose is really a kind of polysaccharide.
Disclosure of Invention
The invention aims to solve the defects in the prior art, such as: the gastrointestinal tract of the human body has limited absorption to plants, such as cellulose in the plants, and the human body cannot directly digest and absorb the plants, so the equipment and the process for extracting the dendrobium huoshanense active substances are provided.
In order to achieve the purpose, the invention adopts the following technical scheme:
an equipment for extracting dendrobium huoshanense active substances comprises a heating tank, a primary centrifuge, a deproteinizing tank, a secondary centrifuge and a chromatography column, wherein an isolation net is installed in the heating tank, a circulation groove is formed in the heating tank, a primary connecting pipe is connected below the heating tank and is connected with the primary centrifuge, a primary collecting tank is arranged below the primary centrifuge, the primary centrifuge is connected with the deproteinizing tank, a water bath tank is formed in the deproteinizing tank, a secondary connecting pipe is connected between the primary collecting tank and the deproteinizing tank, a primary external pipe is arranged at the upper end of the deproteinizing tank, an output pipe is arranged at the lower end of the deproteinizing tank and is connected with the secondary centrifuge, a secondary collecting tank is arranged below the secondary centrifuge and is connected with the chromatography column, a tertiary connecting pipe is arranged between the collecting chamber and the chromatography column, the device comprises a chromatographic column and is characterized in that a split charging device is arranged below the chromatographic column, chromatographic gel is filled in the chromatographic column, a pressurizing device is installed in the chromatographic column, and the upper end of the chromatographic column is connected with an input pipe.
Preferably, one-level centrifuge and second grade centrifuge all include installation shell and mount, mount and installation shell fixed connection, the rotator is installed to the installation shell internal rotation, be provided with first motor in the mount, the drive end fixedly connected with drive shaft of first motor, first motor passes through the drive shaft and is connected with the rotator, the lower extreme of installation shell is provided with outer export, install the inner bearing in the export, the export in has been seted up to the lower extreme of rotator, inner export is inserted and is equipped with sealed post, the lower extreme fixedly connected with bottom plate of sealed post, solid fixed ring has been cup jointed to the bottom plate periphery, install the outer bearing between solid fixed ring and the bottom plate, gu fixed ring fixedly connected with thread bush, thread bush and installation shell threaded connection.
Preferably, all be provided with a plurality of collection chambeies in second grade collection tank and the one-level collection tank, every the collection chamber all communicates there is the output, the bottom of second grade collection tank and one-level collection tank is rotated and is connected with the base, install the second motor in the base, two the drive end of second motor respectively with second grade collection tank, one-level collection tank fixed connection.
Preferably, the partial shipment device includes external fixation case and mount pad, external fixation case fixed connection is at the upside of mount pad, rotatory case in the external fixation incasement rotation is installed, install the third motor in the mount pad.
Preferably, supercharging device includes pressure block and commentaries on classics board, the inner wall sliding connection of pressure block and chromatography post, it rotates and installs in the pressure block to change the board, a plurality of fan-shaped openings have been seted up on the pressure block, it is provided with a plurality of sealed leaves that correspond with fan-shaped opening to change the board, the top fixedly connected with carriage release lever of changeing the board, the carriage release lever passes pressure block and chromatography post, one side of chromatography post is connected with the pressure pipe, be provided with the spacing ring in the chromatography post.
A technology for extracting dendrobium huoshanense active substances comprises the following steps:
s1: weighing fresh dendrobium huoshanense material; adding distilled water into an isolation net of a heating tank according to a material ratio of 1:3(g: ml), heating at 80 ℃, extracting filtrate once every 2 hours for three times, combining the extracting solutions, placing in an evaporator for concentration, and centrifuging by a primary centrifuge at 12000rpm to remove impurities;
s2: deproteinizing the polysaccharide; introducing the extract into a deproteinization tank, adding a trypsin solution, carrying out enzymolysis for 5h in a constant-temperature water bath at 37 ℃, adding a 0.5% TCA solution until the optimum concentration is reached, standing overnight, centrifuging at 12000rpm of a secondary centrifuge to remove protein precipitates, taking supernate, regulating the pH of the supernate to be neutral by using NaOH, carrying out evaporation concentration, adding 95% ethanol (the extract: ethanol is 3:16), carrying out overnight precipitation to obtain polysaccharide, collecting precipitates, dissolving the precipitates with distilled water, repeatedly precipitating to obtain white polysaccharide precipitates, continuously centrifuging by using the secondary centrifuge to collect the precipitates, carrying out freeze drying, and storing in a dryer;
s3: separating by DEAE-cellulose column chromatography; dissolving the precipitate in S2, dissolving polysaccharide solution in double distilled water, filtering insoluble impurities, and separating the filtered solution by DEAE-cellulose column chromatography using chromatographic column; eluting with double distilled water at 5 ml/min-1And collecting by sub-packaging device to obtain 5 peak values, concentrating each main peak to appropriate volume, dialyzing for desalting, and cooling for drying.
Compared with the prior art, the invention has the beneficial effects that:
1. the extraction of polysaccharide active substances in the dendrobium huoshanense can be well realized by the aid of systematic equipment, the active substances are orderly and effectively extracted in a production line working mode, and insoluble cellulose such as protein and the like in the dendrobium huoshanense can be removed through multiple times of filtration.
2. Quantitatively controlling reactants added in the extracting solution, avoiding the loss of polysaccharide as much as possible, removing protein in the extracting solution to the maximum extent, ensuring the purity of the polysaccharide separated and extracted, and separating the polysaccharide according to the molecular mass by a chromatography.
3. According to the amplitude of free movement of different molecular masses, the chromatographic method classifies the separated polysaccharides according to the molecular masses, the polysaccharides are sequentially output from large to small according to the molecular masses, and the polysaccharides can be separated and purified while being extracted.
Drawings
FIG. 1 is a schematic structural diagram of an apparatus for extracting active substances from Dendrobium huoshanense according to the present invention;
FIG. 2 is a schematic structural diagram of a pressurizing device of an active material extraction apparatus for dendrobium huoshanense according to the present invention;
FIG. 3 is a schematic view of the structure at A in FIG. 1;
FIG. 4 is a schematic view of the structure at B in FIG. 1;
fig. 5 is a schematic structural diagram at C in fig. 1.
In the figure: 1 heating tank, 2 isolating net, 3 circulating tank, 4 second grade connecting pipe, 5 first grade external connecting pipe, 6 deproteinizing tank, 7 water bath tank, 8 output pipe, 9 first motor, 10 fixed mount, 11 driving shaft, 12 second grade centrifuge, 13 sealing column, 14 rotator, 15 mounting shell, 16 second grade collecting tank, 17 collecting cavity, 18 output end, 19 base, 20 second motor, 21 sector opening, 22 rotating plate, 23 pressure block, 24 bottom plate, 25 third grade connecting pipe, 26 chromatography column, 27 chromatography gel, 28 moving rod, 29 input pipe, 30 through pressure pipe, 31 first grade connecting pipe, 32 first grade collecting tank, 33 external fixed box, 34 mounting seat, 35 third motor, 36 internal rotating box, 37 screw sleeve, 38 external bearing, fixed ring 39, 40 internal bearing, 41 first grade centrifuge.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-5, an apparatus for extracting active substances from dendrobium huoshanense and a process thereof are as follows:
an active substance extraction device of dendrobium huoshanense comprises a heating tank 1, a primary centrifuge 41, a deproteinizing tank 6, a secondary centrifuge 12 and a chromatography column 26, wherein an isolation net 2 is installed in the heating tank 1, a circulation groove 3 is formed in the heating tank 1, a primary connecting pipe 31 is connected below the heating tank 1, the primary connecting pipe 31 is connected with the primary centrifuge 41, a primary collecting tank 32 is arranged below the primary centrifuge 41, the primary centrifuge 41 is connected with the deproteinizing tank 6, a water bath 7 is formed in the deproteinizing tank 6, a secondary connecting pipe 4 is connected between the primary collecting tank 32 and the deproteinizing tank 6, a primary external connecting pipe 5 is arranged at the upper end of the deproteinizing tank 6, an output pipe 8 is arranged at the lower end of the deproteinizing tank 6, the output pipe 8 is connected with the secondary centrifuge 12, a secondary collecting tank 16 is arranged below the secondary centrifuge 12, and the secondary collecting tank 16 is, a three-stage connecting pipe 25 is arranged between the collecting cavity 17 and the chromatographic column 26, a split charging device is arranged below the chromatographic column 26, chromatographic gel 27 is filled in the chromatographic column 26, a pressurizing device is arranged in the chromatographic column 26, and the upper end of the chromatographic column 26 is connected with an input pipe 29;
the primary centrifugal machine 41 and the secondary centrifugal machine 12 both comprise a mounting shell 15 and a fixing frame 10, the fixing frame 10 is fixedly connected with the mounting shell 15, a rotating body 14 is rotatably mounted in the mounting shell 15, a first motor 9 is arranged in the fixing frame 10, a driving end of the first motor 9 is fixedly connected with a driving shaft 11, the first motor 9 is connected with the rotating body 14 through the driving shaft 11, an outer outlet is arranged at the lower end of the mounting shell 15, an inner bearing 40 is mounted in the outer outlet, an inner outlet is formed in the lower end of the rotating body 14, a sealing column 13 is inserted into the inner outlet, a bottom plate 24 is fixedly connected to the lower end of the sealing column 13, a fixing ring 39 is sleeved on the periphery of the bottom plate 24, an outer bearing 38 is mounted between the fixing ring 39 and the bottom plate 24;
wherein, a plurality of collecting cavities 17 are arranged in the secondary collecting tank 16 and the primary collecting tank 32, each collecting cavity 17 is communicated with an output end 18, the bottoms of the secondary collecting tank 16 and the primary collecting tank 32 are rotatably connected with a base 19, a second motor 20 is arranged in the base 19, the driving ends of the two second motors 20 are respectively fixedly connected with the secondary collecting tank 16 and the primary collecting tank 32, the subpackaging device comprises an outer fixing box 33 and a mounting seat 34, the outer fixing box 33 is fixedly connected with the upper side of the mounting seat 34, an inner rotating box 36 is rotatably arranged in the outer fixing box 33, a third motor 35 is arranged in the mounting seat 34, the pressurizing device comprises a pressure block 23 and a rotating plate 22, the pressure block 23 is slidably connected with the inner wall of the chromatographic column 26, the rotating plate 22 is rotatably arranged in the pressure block 23, a plurality of fan-shaped openings 21 are arranged on the pressure block 23, the rotating plate 22 is provided with a plurality of sealing leaves corresponding to the fan-, a movable rod 28 is fixedly connected above the rotating plate 22, the movable rod 28 penetrates through the pressure block 23 and the chromatographic column 26, one side of the chromatographic column 26 is connected with a pressure connecting pipe 30, and a limit ring is arranged in the chromatographic column 26.
S1: weighing fresh dendrobium huoshanense material; adding distilled water into an isolation net 2 of a heating tank 1 according to a material ratio of 1:3(g: ml), heating at 80 ℃, extracting filtrate once every 2 hours for three times, combining the extracting solutions, placing the extracting solutions in an evaporator for concentration, and centrifuging by a primary centrifuge 41 at 12000rpm to remove impurities;
disconnecting the heating tank 1 from the first-stage connecting pipe 31, placing the mixture of the dendrobium candidum and the distilled water in the isolation net 2, introducing water temperature of 80 ℃ into the circulating groove 3, and retaining plant fixing fibers of the dendrobium candidum inside the isolation net 2; concentrated solution lets in one-level centrifuge 41, and installation shell 15 and rotator 14 upper end one side in one-level centrifuge 41 all are provided with corresponding port in order to supply the input of solution, and solid impurity subsides in the bottom after the centrifugation, and the upper strata is the clear liquid, and in the supernatant passed through port and second grade connecting pipe 4 input deproteinization jar 6, the lower extreme through one-level centrifuge 41 was exported in the one-level collection tank 32 and is collected to the impurity of subsiding.
The centrifugation process is that the first motor 9 on the first-stage centrifuge 41 drives the internal rotator 14 to rotate through the driving shaft 11 for centrifugation, substances with different masses will be layered in the process of high-speed operation, the sealing post 13 plugs the internal outlet at the lower end of the rotator 14, in the centrifugation process, the internal bearing 40 separates the rotator 14 from the mounting shell 15, the bottom plate 24 rotates along with the sealing post 13, the fixing ring 39 is fixedly connected with the mounting shell 15 through the thread of the thread bushing 37, so as to ensure the stability of the horizontal position of the sealing post 13, if the internal substances are output from the lower end, the bottom plate 24 and the fixing ring 39 need to rotate synchronously, so that the thread bushing 37 is separated from the mounting shell 15, the sealing post 13 can be taken out from the internal outlet of the rotator 14, thereby realizing the output of the internal substances, the second motor 20 in the base 19 drives the first-stage collection tank 32 to rotate through the driving end, when solid settlement layers with, will be collected in the different collecting chambers 17.
S2: deproteinizing the polysaccharide; introducing the extract into a deproteinizing tank 6, adding a trypsin solution, carrying out enzymolysis for 5h in a constant-temperature water bath at 37 ℃, adding a proper amount of 0.5% TCA solution, standing, then dissolving polysaccharide in the solution, allowing protein to react with the TCA solution to form precipitate, standing overnight, centrifuging by a secondary centrifuge 12 at 12000rpm to remove protein precipitate, taking supernate, adjusting the pH of the supernate to be neutral by NaOH, using the secondary centrifuge 12 and a secondary collection tank 16 as a primary centrifuge 41 and a primary collection tank 32, carrying out evaporation concentration, adding 95% ethanol (the extract is ethanol: 3:16), precipitating for at least 12h to obtain polysaccharide, collecting precipitate, dissolving the precipitate with distilled water, repeatedly precipitating to obtain white polysaccharide precipitate, continuously centrifuging by the secondary centrifuge 12, collecting the precipitate, freeze-drying, and storing in a dryer;
s3: separating by DEAE-cellulose column chromatography; dissolving the precipitate in S2, dissolving the polysaccharide solution in double distilled water, and separating the dissolved solution by DEAE-cellulose column chromatography using chromatographic column 26; eluting with double distilled water, and eluting with 0.1mol/L NaCl at 5 ml/min-1Collecting by sub-packaging device to obtain 5 peak values, concentrating each main peak to appropriate volume, dialyzing for desalting, cooling and drying;
finally, inputting the solution with dissolved precipitates into a chromatographic column 26 through a three-stage connecting pipe 25, firstly inputting double distilled water through a pressure connecting pipe 30, wherein the material of a chromatographic gel 27 is DEAE-cellulose, rotating a rotating plate 22 through a moving rod 28, inputting the solution into the chromatographic column 26 when a fan-shaped opening 21 is staggered with a sealing leaf of the rotating plate 22, and after inputting the solution, continuing to rotate the moving rod 28 to ensure that the sealing leaf of the rotating plate 22 is superposed with the fan-shaped opening 21, sealing the upper end of the chromatographic column 26, and internally introducing gas through the pressure connecting pipe 30, wherein an input pipe 29 and the three-stage connecting pipe 25 are disconnected by a valve, and a pressure block 23 slides downwards in the chromatographic column 26 under the action of air pressure;
when the solution passes through the chromatographic gel 27 under pressure, due to the different masses of the individual molecules, according to the free movement rule of molecules, the polysaccharide molecules with smaller mass freely move in the chromatographic gel 27 and stay for longer time, for polysaccharide molecules with larger mass, the space is narrow, the motion amplitude is obviously reduced, the polysaccharide molecules can rapidly pass through the chromatographic gel 27, the polysaccharides with different molecular masses in the solution flow out from large to small in sequence due to the difference of the molecular masses, the flowing solution is collected by the inner rotary box 36, wherein the external fixed box 33 can automatically detect the solutions with different peak values, when the peak value changes, the third motor 35 drives the internal rotating box 36 to rotate through the driving end, so as to separate the solutions with different peak values, namely different molecular masses, and after the solutions are eluted and separated by using double distilled water, then, the separated solution was eluted again with 0.1mol/L NaCl.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The utility model provides a Huoshan huoshan equipment of drawinging, includes heating jar (1), one-level centrifuge (41), deproteinization jar (6), second grade centrifuge (12) and chromatography post (26), its characterized in that, install separation net (2) in heating jar (1), set up circulation groove (3) in heating jar (1), the below of heating jar (1) is connected with one-level connecting pipe (31), one-level connecting pipe (31) are connected with one-level centrifuge (41), the below of one-level centrifuge (41) is provided with one-level collecting tank (32), one-level centrifuge (41) and deproteinization jar (6) are connected, set up water bath (7) in deproteinization jar (6), be connected with second grade connecting pipe (4) between one-level collecting tank, the lower extreme of deproteinization jar (6) is provided with output tube (8), output tube (8) are connected with second grade centrifuge (12), the below of second grade centrifuge (12) is provided with second grade holding vessel (16), second grade holding vessel (16) are connected with chromatography column (26), be provided with tertiary connecting pipe (25) between collection chamber (17) and chromatography column (26), the below of chromatography column (26) is provided with the partial shipment device, chromatography column (26) intussuseption is filled with chromatography gel (27), install supercharging device in chromatography column (26), the upper end of chromatography column (26) is connected with input tube (29).
2. The dendrobium huoshanense active substance extraction device according to claim 1, wherein each of the primary centrifugal machine (41) and the secondary centrifugal machine (12) comprises a mounting shell (15) and a fixing frame (10), the fixing frame (10) is fixedly connected with the mounting shell (15), a rotating body (14) is rotatably mounted in the mounting shell (15), a first motor (9) is arranged in the fixing frame (10), a driving shaft (11) is fixedly connected with a driving end of the first motor (9), the first motor (9) is connected with the rotating body (14) through the driving shaft (11), an outer outlet is formed in the lower end of the mounting shell (15), an inner bearing (40) is mounted in the outer outlet, an inner outlet is formed in the lower end of the rotating body (14), a sealing column (13) is inserted in the inner outlet, a bottom plate (24) is fixedly connected with the lower end of the sealing column (13), fixed ring (39) has been cup jointed to bottom plate (24) periphery, install outer bearing (38) between fixed ring (39) and bottom plate (24), fixed ring (39) fixedly connected with thread bush (37), thread bush (37) and installation shell (15) threaded connection.
3. The dendrobe active substance extraction equipment as claimed in claim 1, wherein a plurality of collection cavities (17) are arranged in each of the secondary collection tank (16) and the primary collection tank (32), each collection cavity (17) is communicated with an output end (18), a base (19) is rotatably connected to the bottoms of the secondary collection tank (16) and the primary collection tank (32), a second motor (20) is installed in each base (19), and the driving ends of the two second motors (20) are fixedly connected with the secondary collection tank (16) and the primary collection tank (32) respectively.
4. The dendrobe active substance extracting equipment as claimed in claim 1, wherein the split charging device comprises an outer fixed box (33) and a mounting seat (34), the outer fixed box (33) is fixedly connected to the upper side of the mounting seat (34), an inner rotating box (36) is rotatably mounted in the outer fixed box (33), and a third motor (35) is mounted in the mounting seat (34).
5. The dendrobium huoshanense active substance extraction equipment according to claim 1, wherein the pressurization device comprises a pressure block (23) and a rotating plate (22), the pressure block (23) is in sliding connection with the inner wall of the chromatography column (26), the rotating plate (22) is rotatably installed in the pressure block (23), a plurality of fan-shaped openings (21) are formed in the pressure block (23), a plurality of sealing leaves corresponding to the fan-shaped openings (21) are arranged on the rotating plate (22), a moving rod (28) is fixedly connected to the upper portion of the rotating plate (22), the moving rod (28) penetrates through the pressure block (23) and the chromatography column (26), a pressure connecting pipe (30) is connected to one side of the chromatography column (26), and a limit ring is arranged in the chromatography column (26).
6. The process for extracting the active substances of dendrobium huoshanense is characterized by comprising the following steps:
s1: weighing fresh dendrobium huoshanense material; adding distilled water into an isolation net (2) of a heating tank (1) according to a material ratio of 1:3(g: ml), heating at 80 ℃, extracting filtrate once every 2h for three times, combining extracting solutions, placing the extracting solutions in an evaporator for concentration, and centrifuging by a primary centrifuge (41) at 12000rpm to remove impurities;
s2: deproteinizing the polysaccharide; introducing the extract into a deproteinization tank (6), adding a trypsin solution, carrying out enzymolysis for 5h in a constant-temperature water bath at 37 ℃, adding a 0.5% TCA solution until the optimum concentration is reached, standing overnight, centrifuging at 12000rpm of a secondary centrifuge (12) to remove protein precipitates, taking supernate, adjusting the pH of the supernate to be neutral by using NaOH, carrying out evaporation concentration, adding 95% ethanol (the ethanol is the extract: 3:16), precipitating overnight to obtain polysaccharide, collecting precipitates, dissolving the precipitates by using distilled water, repeatedly precipitating to obtain white polysaccharide precipitates, continuously centrifuging the secondary centrifuge (12), collecting the precipitates, freezing and drying, and storing in a dryer;
s3: separating by DEAE-cellulose column chromatography;dissolving the precipitate in S2, dissolving polysaccharide solution in double distilled water, filtering insoluble impurities, separating the filtered solution with chromatography column (26) by DEAE-cellulose column chromatography; eluting with double distilled water at 5 ml/min-1And collecting by sub-packaging device to obtain 5 peak values, concentrating each main peak to appropriate volume, dialyzing for desalting, and cooling for drying.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115364518A (en) * | 2022-09-16 | 2022-11-22 | 石河子大学 | Process and device for extracting cAMP from Xinjiang jun dates |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203174011U (en) * | 2013-04-13 | 2013-09-04 | 李苹苹 | Mussel polysaccharide extraction system |
| CN204727818U (en) * | 2015-06-29 | 2015-10-28 | 吉首大学 | A kind of device extracting polysaccharide and naringenin from Herba Dendrobii |
| CN206396103U (en) * | 2017-01-12 | 2017-08-11 | 广州瑞信化妆品有限公司 | A kind of extraction element of cross-linking sodium hyaluronate gel |
| CN110016088A (en) * | 2019-04-26 | 2019-07-16 | 安徽利民生物科技股份有限公司 | A kind of ganoderma lucidum polysaccharide extraction process device |
-
2019
- 2019-10-23 CN CN201911010457.XA patent/CN110669150B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203174011U (en) * | 2013-04-13 | 2013-09-04 | 李苹苹 | Mussel polysaccharide extraction system |
| CN204727818U (en) * | 2015-06-29 | 2015-10-28 | 吉首大学 | A kind of device extracting polysaccharide and naringenin from Herba Dendrobii |
| CN206396103U (en) * | 2017-01-12 | 2017-08-11 | 广州瑞信化妆品有限公司 | A kind of extraction element of cross-linking sodium hyaluronate gel |
| CN110016088A (en) * | 2019-04-26 | 2019-07-16 | 安徽利民生物科技股份有限公司 | A kind of ganoderma lucidum polysaccharide extraction process device |
Non-Patent Citations (1)
| Title |
|---|
| 冯宝军: ""霍山石斛多糖的分离纯化及结构鉴定"", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115364518A (en) * | 2022-09-16 | 2022-11-22 | 石河子大学 | Process and device for extracting cAMP from Xinjiang jun dates |
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