CN110665045B - Gynaecology and obstetrics is with absorbing stylolite - Google Patents
Gynaecology and obstetrics is with absorbing stylolite Download PDFInfo
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- CN110665045B CN110665045B CN201910986103.2A CN201910986103A CN110665045B CN 110665045 B CN110665045 B CN 110665045B CN 201910986103 A CN201910986103 A CN 201910986103A CN 110665045 B CN110665045 B CN 110665045B
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/06—At least partially resorbable materials
- A61L17/10—At least partially resorbable materials containing macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/06—At least partially resorbable materials
- A61L17/10—At least partially resorbable materials containing macromolecular materials
- A61L17/12—Homopolymers or copolymers of glycolic acid or lactic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/14—Post-treatment to improve physical properties
- A61L17/145—Coating
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/02—Methods for coating medical devices
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Abstract
The invention discloses an absorbable suture for obstetrics and gynecology department, which is prepared by using a chitosan silk thread as a core thread and a modified polylactic acid silk thread as a shell thread, stranding and weaving the chitosan silk thread and the shell thread, immersing the chitosan silk thread into a coating liquid containing calcium stearate and polylactic acid, and drying the coating liquid. Through the mode, the mechanical property and the hydrophilic property of the polylactic acid can be improved, so that the prepared suture has higher strength and good biocompatibility; the chitosan in the suture is slowly degraded, so that the suture can gradually form a hollow structure while the long-acting antibacterial effect is achieved, the tissue ingrowth healing is promoted, and the effect of accelerating the tissue healing is achieved; and the whole preparation process is simple and convenient to control, and can meet the requirements of actual production and application.
Description
Technical Field
The invention relates to the technical field of medical sutures, in particular to an absorbable suture for obstetrics and gynecology department.
Background
In the clinical diagnosis and treatment of obstetrics and gynecology department, the operation has irreplaceable effect, and the suture line is one of the necessary materials for suturing the incision after the operation, and has important significance for accelerating the healing of the incision and reducing postoperative complications. At present, the suture line is generally divided into an absorbable suture line and a non-absorbable suture line, wherein the absorbable suture line is non-toxic, non-irritant, high in compatibility, good in biodegradability, capable of being degraded and absorbed by a human body, free of the pain of stitches removing of a patient, and capable of achieving the effect of enhancing the attractive operation, so that the absorbable suture line is widely applied to clinical operation suture, particularly to gynecological clinical practice, and meets the requirements of doctors and patients.
Currently, absorbable sutures for obstetrics and gynecology department are mainly divided into natural fiber sutures and artificial synthetic fiber sutures, and the types of raw materials of the sutures and the preparation method thereof have important influence on the performance of the prepared absorbable sutures. Patent publication No. CN102406961B provides an absorbable surgical suture and a method for preparing the same, the absorbable surgical suture is a chitosan surgical suture, which is a natural fiber suture; according to the method, the toughness of the chitosan is improved by adding urea and glycerol into a chitosan solution, and the cross section roundness of the suture is improved and the operability of the suture is improved by adopting a wet spinning process. However, because the chitosan is degraded at a high speed, the mechanical strength of the chitosan is rapidly reduced in the actual use process, so that the chitosan is difficult to maintain enough strength before the wound of a patient is recovered, secondary cracking of the wound is easily caused, and the recovery of the patient is affected.
Patent publication No. CN106039388A provides a method for preparing a polylactic acid-based surgical suture, the absorbable suture is a synthetic fiber suture with polylactic acid as a main component; according to the method, polylactic acid and various absorbable materials are blended, so that the effects of improving the mechanical property and the antibacterial rate of the suture are achieved. However, the variety of the raw materials used in the patent is too many, and the raw materials are only simply mixed, so that the action effect is unclear, and the performance of the suture is difficult to effectively regulate and control; meanwhile, since the degradation speed of polylactic acid is slow, although high strength can be maintained, the interior of the obtained suture line continuously keeps a compact structure, and the ingrowth of tissues is limited, so that the healing time is prolonged, and the healing effect is influenced.
Therefore, there is still a need for an absorbable suture for obstetrics and gynecology department that can maintain high strength, promote tissue growth, and accelerate tissue healing.
Disclosure of Invention
The invention aims to solve the problems and provides an absorbable suture for obstetrics and gynecology department and a preparation method thereof, wherein the absorbable suture is formed by weaving a chitosan thread as a core thread and a modified polylactic acid thread as a shell thread, so that the mechanical property and the hydrophilic property of polylactic acid can be improved, and the prepared suture has higher strength and good biocompatibility; the degradation speed of the chitosan can be reduced, the slow release effect is achieved, and the suture line gradually forms a hollow structure along with the degradation of the chitosan, so that the tissue ingrowth healing is promoted, and the effect of accelerating the tissue healing is achieved.
In order to achieve the aim, the invention provides an absorbable suture for obstetrics and gynecology department, which comprises a core wire and a multi-strand shell wire, wherein the multi-strand shell wire is braided around the core wire, and the outer side of the multi-strand shell wire is covered with a coating; the core wire is a chitosan wire, the shell wire is a modified polylactic acid wire, and the coating comprises calcium stearate and polylactic acid.
Furthermore, the diameter of the core wire is 0.1-0.2 mm, and the diameter of the shell wire is 0.06-0.2 mm.
Furthermore, the number of strands of the multi-strand shell line is 6-8 strands.
Further, the deacetylation degree of the chitosan is 90%, and the modified polylactic acid is chitosan grafted modified polylactic acid.
Further, the mass ratio of calcium stearate to polylactic acid in the coating is 1: 1.
In order to achieve the above object, the present invention further provides a method for preparing an absorbable suture for obstetrics and gynecology department, comprising the following steps:
s1, dissolving chitosan in a mixed solution containing acetic acid and urea, filtering and degassing to obtain a spinning solution, and preparing a chitosan yarn by wet spinning;
s2, mixing the lactic acid solution with the polyethylene glycol solution, preparing polylactic acid by adopting a solution polycondensation method, mixing and heating the prepared polylactic acid and chitosan to prepare chitosan grafted modified polylactic acid, and preparing modified polylactic acid threads by adopting a melt spinning method;
s3, taking the chitosan silk thread obtained in the step S1 as a core thread, taking the modified polylactic acid silk thread obtained in the step S2 as a shell thread, and performing stranding knitting by adopting a knitting machine to obtain a knitting thread;
s4, mixing calcium stearate and polylactic acid, and dissolving in absolute ethyl alcohol to prepare a coating solution; and soaking the braided wire prepared in the step S3 into the coating liquid, taking out and drying after fully soaking, and preparing the absorbable suture for obstetrics and gynecology department.
Further, in step S1, the mass fraction of acetic acid in the mixed solution is 4% to 7%, the mass fraction of urea is 1% to 3%, and the balance is water.
Further, in step S1, the mass ratio of the chitosan to the mixed solution is 1 (15-25).
Further, in step S2, the concentration of the lactic acid solution is 88%, the concentration of the polyethylene glycol solution is 50%, and the mass ratio of the lactic acid solution to the polyethylene glycol solution is (0.8-1.2): 1.
Further, in step S2, the mass ratio of the polylactic acid to the chitosan is (12-15): 1.
Compared with the prior art, the invention has the beneficial effects that:
1. the absorbable suture for obstetrics and gynecology department provided by the invention takes the chitosan silk thread as the core thread and takes the modified polylactic acid silk thread as the shell thread, and the absorbable suture is formed by weaving, so that the mechanical property and the hydrophilic property of polylactic acid can be improved, and the prepared suture has higher strength and good biocompatibility; the degradation speed of the chitosan can be reduced, a slow release effect is achieved, and the suture line gradually forms a hollow structure along with the degradation of the chitosan, so that the tissue ingrowth healing is promoted, and the effect of accelerating the tissue healing is achieved; and the whole preparation process is simple and convenient to control, and can meet the requirements of actual production and application.
2. According to the invention, polyethylene glycol is introduced in the process of preparing polylactic acid, and the polylactic acid is subjected to hydroxyl-terminated modification, so that the prepared polylactic acid has end group activity; at the moment, chitosan is introduced, so that the chitosan can be fully grafted on the active end group of the polylactic acid; the prepared chitosan grafted modified polylactic acid can improve the mechanical property of the polylactic acid, so that the prepared suture has higher strength and provides enough support for wounds; the hydrophilic property of the polylactic acid can be obviously improved, the antibacterial effect is achieved, the inflammatory reaction caused by the hydrophobic property of the polylactic acid is avoided, and the use safety of a patient is guaranteed.
3. According to the invention, the modified polylactic acid silk thread with a slow degradation speed is used as the shell thread, so that the suture thread can keep high strength, thereby playing an effective supporting role before the wound is healed and avoiding the secondary cracking of the wound; meanwhile, the chitosan silk thread with high degradation speed is used as the core thread, so that the chitosan can be prevented from being rapidly degraded and slowly released, the long-acting antibacterial effect can be achieved, and the hollow structure can be gradually formed in the suture thread along with the degradation of the chitosan, so that the tissue ingrowth is promoted, and the healing is accelerated.
4. According to the invention, the prepared suture is coated by the coating liquid prepared by mixing calcium stearate and polylactic acid, so that the smoothness of the surface of the suture can be improved by using the calcium stearate in the coating, the dragging effect of the suture on tissues is reduced, and the wound infection area is reduced; and the polylactic acid in the coating can be utilized to achieve high-efficiency compatibility with the shell line, so that the infiltration of the coating liquid is promoted, the gap between the shell lines is fully filled, and the uniform and stable coating effect is achieved.
Drawings
FIG. 1 is a schematic cross-sectional view of an absorbable suture for obstetrics and gynecology department prepared in example 1 of the present invention;
the parts in the drawings are numbered as follows: 1. a core wire; 2. a shell line; 3. and (4) coating.
Detailed Description
The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention easier to understand by those skilled in the art, and thus will clearly and clearly define the scope of the invention. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.
The invention provides an absorbable suture for obstetrics and gynecology department, which comprises a core wire and a plurality of strands of shell wires woven around the core wire, wherein the outer sides of the strands of shell wires are coated with coatings; the core wire is a chitosan wire, the shell wire is a modified polylactic acid wire, and the coating comprises calcium stearate and polylactic acid.
The diameter of the core wire is 0.1-0.2 mm, and the diameter of the shell wire is 0.06-0.2 mm.
The number of strands of the multi-strand shell line is 6-8 strands.
The deacetylation degree of the chitosan is 90%, and the modified polylactic acid is chitosan grafted modified polylactic acid.
The mass ratio of calcium stearate to polylactic acid in the coating is 1: 1.
The invention also provides a preparation method of the absorbable suture for obstetrics and gynecology department, which comprises the following steps:
s1, dissolving chitosan in a mixed solution containing acetic acid and urea, filtering and degassing to obtain a spinning solution, and preparing a chitosan yarn by wet spinning;
s2, mixing the lactic acid solution with the polyethylene glycol solution, preparing polylactic acid by adopting a solution polycondensation method, mixing and heating the prepared polylactic acid and chitosan to prepare chitosan grafted modified polylactic acid, and preparing modified polylactic acid threads by adopting a melt spinning method;
s3, taking 1 strand of the chitosan silk thread prepared in the step S1 as a core thread, taking 6-8 strands of the modified polylactic acid silk thread prepared in the step S2 as a shell thread, and performing stranding and weaving by adopting a weaving machine to prepare a braided thread;
s4, mixing calcium stearate and polylactic acid according to the mass ratio of 1:1, and dissolving in absolute ethyl alcohol to prepare coating liquid; and soaking the braided wire prepared in the step S3 into the coating liquid, taking out and drying after fully soaking, and preparing the absorbable suture for obstetrics and gynecology department.
In step S1, the mass fraction of acetic acid in the mixed solution is 4% -7%, the mass fraction of urea is 1% -3%, and the balance of water; the mass ratio of the chitosan to the mixed solution is 1 (15-25).
In step S2, the concentration of the lactic acid solution is 88%, the concentration of the polyethylene glycol solution is 50%, and the mass ratio of the lactic acid solution to the polyethylene glycol solution is (0.8-1.2): 1; the mass ratio of the polylactic acid to the chitosan is (12-15): 1.
The following describes a method for producing an absorbable suture for obstetrics and gynecology department of the present invention with reference to examples 1 to 13 and comparative examples 1 to 6:
example 1
The embodiment provides a preparation method of an absorbable suture for obstetrics and gynecology department, which comprises the following steps:
s1, 5g of the compound (I) has a degree of deacetylation of 90% and a relative molecular mass of > 1.0X 106The chitosan is dissolved in 100g of mixed solution containing acetic acid and urea, wherein the mass fraction of the acetic acid in the mixed solution is 5%, and the mass fraction of the urea is 2%; fully stirring to obtain a chitosan solution, filtering and removing bubbles to obtain a spinning solution, spraying the spinning solution through a spinneret plate, and then solidifying and stretching to obtain a chitosan silk thread with the diameter of 0.1 mm;
s2, uniformly mixing 88% lactic acid solution and 50% polyethylene glycol solution according to the mass ratio of 1:1, heating at 70 ℃ for 2h to remove water, and adding N2Heating the mixture to 120 ℃ in an oil bath under the atmosphere, prepolymerizing the mixture for 2 hours, adding stannous octoate serving as a catalyst, heating the mixture to 150 ℃, and preserving the temperature for 6 hours to obtain polylactic acid; mixing the prepared polylactic acid and chitosan according to the mass ratio of 12:1, heating at 80 ℃ for 2h, filtering, washing and drying to prepare chitosan grafted modified polylactic acid; then putting the prepared chitosan grafted modified polylactic acid into a screw extruder, melting at 200 ℃, spraying out through a spinneret plate, and solidifying and stretching to prepare a modified polylactic acid silk thread with the diameter of 0.2 mm;
s3, taking 1 strand of the chitosan silk thread prepared in the step S1 as a core thread, taking 8 strands of the modified polylactic acid silk thread prepared in the step S2 as a shell thread, and performing stranding and weaving by adopting a weaving machine to prepare a braided thread;
s4, mixing calcium stearate and polylactic acid according to the mass ratio of 1:1, and dissolving in absolute ethyl alcohol to prepare coating liquid; and soaking the braided wire prepared in the step S3 in the coating liquid for 10min at normal temperature, taking out, and drying at 50 ℃ for 2h to prepare the absorbable suture for obstetrics and gynecology department.
The schematic cross-sectional structure of the absorbable suture for obstetrics and gynecology manufactured in this example is shown in fig. 1, and comprises a core wire 1 and 8 strands of shell wires 2 braided around the core wire, wherein the outer sides of the 8 strands of shell wires are covered with a coating 3.
The prepared absorbable suture for obstetrics and gynecology department is sampled, a single-yarn electronic force machine is adopted, the stretching speed is set to be 20mm/min, and the breaking strength and the breaking elongation of the prepared absorbable suture sample for obstetrics and gynecology department are respectively tested for representing the mechanical property of the prepared absorbable suture.
The prepared absorbable suture for obstetrics and gynecology department is subjected to an antibacterial test by adopting an oscillation method, and the antibacterial rate is calculated by respectively measuring the concentration of staphylococcus aureus of a solution containing the absorbable suture sample before oscillation and after oscillation for 18 hours so as to represent the antibacterial performance of the prepared absorbable suture.
The prepared absorbable suture line for obstetrics and gynecology department is placed in a simulated human body fluid to carry out an in-vitro degradation test, the absorbable suture line is dried, disassembled and weighed, the mass loss rate of the core line and the shell line of the absorbable suture line is respectively tested when the absorbable suture line is degraded on the 7 th day, the 14 th day and the 21 st day, and the mechanical property and the antibacterial property of the absorbable suture line are tested according to the test method for representing the degradation property of the prepared absorbable suture line.
The results of the mechanical property, antibacterial property and degradation property tests of the absorbable suture thread for obstetrics and gynecology department prepared in the embodiment are shown in table 1.
TABLE 1 Performance parameters of the gynecological absorbable suture prepared in example 1
As can be seen from Table 1, the absorbable suture for obstetrics and gynecology department prepared by the embodiment has higher breaking strength, elongation at break and bacteriostasis rate when not beginning to degrade, and the suture has better mechanical property and antibacterial property. After a period of time of degradation, the core wire and the shell wire of the suture begin to generate mass loss, and the breaking strength, the breaking elongation and the bacteriostasis rate of the suture are all reduced, wherein the initial reduction amplitude of the breaking strength and the breaking elongation is small, so that the suture can keep high strength and elasticity in the initial stage of suture, and the suture can play an effective supporting role on wounds; the whole change of the bacteriostasis rate is small, which shows that the suture can play a long-acting bacteriostasis role. Meanwhile, along with the increase of the degradation time, the mass loss speed of the core wire is greater than that of the shell wire, so that the suture gradually forms a hollow structure, the tissue ingrowth healing can be promoted, and the healing speed is accelerated. Therefore, the absorbable suture line for obstetrics and gynecology department prepared by the embodiment has better mechanical property and antibacterial property, can play a role in promoting tissue healing, and can meet the requirements of practical application.
Examples 2 to 4 and comparative examples 1 to 2
Examples 2 to 4 and comparative examples 1 to 2 respectively provide a method for preparing an absorbable suture thread for obstetrics and gynecology department, which is different from example 1 in that the diameters of the core thread and the shell thread and the number of strands of the shell thread are changed; the core wire diameter, the sheath wire diameter and the number of sheath wires corresponding to each of the examples and comparative examples are shown in Table 2.
TABLE 2 core wire diameter, case wire diameter and number of case wire strands corresponding to examples 2 to 4 and comparative examples 1 to 2
The mechanical properties, antibacterial properties and degradation properties of the absorbable sutures for obstetrics and gynecology department prepared in examples 2 to 4 and comparative examples 1 to 2 were tested, and the results are shown in table 3.
TABLE 3 Performance parameters of the absorbable suture for obstetrics and gynecology department prepared in examples 2 to 4 and comparative examples 1 to 2
As can be seen from Table 3, varying the core wire diameter, the shell wire diameter and the number of shell wire strands has an effect on the mechanical, antimicrobial and degradation properties of the resulting absorbable suture. Comparing examples 1-2 and examples 3-4 respectively, it can be seen that when the number of strands of the shell thread is the same, the diameter of the core thread and the diameter of the shell thread are increased, which is beneficial to improving the mechanical property of the prepared suture thread, and has little influence on the antibacterial property and the degradation property; however, the suture line is too thick due to the excessive core wire diameter and the shell wire diameter, and the wound size of a patient is increased, so the core wire diameter is preferably 0.1-0.2 mm, and the shell wire diameter is preferably 0.06-0.2 mm.
It can be seen from comparison example 1 and comparison examples 1-2 that when the diameter of the core wire is the same as that of the shell wire, the number of strands of the shell wire is increased, the mechanical property of the suture can be improved, and the antibacterial property and the degradation property can be reduced. The core wire is difficult to fully wrap when the number of the shell wires is small, the integral compactness of the suture is poor, the core wire is easy to expose outside, the degradation speed is accelerated, and the mechanical property and the antibacterial property of the suture are quickly reduced in a short time; when the number of strands of the shell thread is large, the shell thread wraps the core thread too tightly, so that the core thread is degraded at a low speed at the moment, the bacteriostatic effect is influenced and the tissue ingrowth is hindered although the mechanical property of the suture thread can be improved. Therefore, the number of strands of the multi-strand sheath wire is preferably 6 to 8.
Comparative examples 3 to 5
Comparative examples 3 to 5 provide a method for preparing an absorbable suture thread for obstetrics and gynecology department, which is different from example 1 in that the materials of the core thread and the shell thread are changed. Wherein, the core wire and the shell wire of the comparative example 3 are both chitosan silk threads, the core wire and the shell wire of the comparative example 4 are both modified polylactic acid silk threads, the core wire of the comparative example 5 is chitosan silk thread, and the shell wire is common polylactic acid silk thread; the mechanical properties, antibacterial properties and degradation properties of the gynecological sutures prepared in various proportions are shown in table 4.
TABLE 4 Performance parameters of the absorbable suture for obstetrics and gynecology department prepared in comparative examples 3 to 5
As can be seen from Table 4, the absorbable sutures for obstetrics and gynecology department prepared in comparative examples 3 to 5 have relatively poor comprehensive properties compared with example 1. When the core wire and the shell wire are both chitosan silk wires, the initial strength of the prepared suture is low and the amplitude reduction is large due to poor mechanical property and high degradation speed of chitosan; although the antibacterial performance of chitosan is excellent, the antibacterial effect is short along with the rapid degradation of chitosan. When the core wire and the shell wire are both modified polylactic acid silk threads, the prepared suture can continuously keep higher strength due to better mechanical property and slower degradation speed, but the antibacterial effect is general due to the lack of chitosan; and because the degradation speed in the suture is too slow, the tissue ingrowth is hindered, and the healing of the tissue is influenced. When the common polylactic acid silk thread is adopted as the shell thread, the prepared suture thread has relatively poor mechanical properties, relatively low degradation speed, lack of the effect of chitosan in an initial state and poor antibacterial effect.
Examples 5 to 10
Examples 5 to 10 respectively provide a method for preparing an absorbable suture thread for obstetrics and gynecology department, which is different from example 1 in that the mass fractions of acetic acid and urea in the mixed solution in step S1 and the mass ratio of chitosan to the mixed solution are changed. Since the change of the relevant parameters in step S1 only affects the core wire and mainly shows the change of the antibacterial performance, the antibacterial performance of the absorbable suture for gynaecological delivery prepared in examples 5 to 10 was tested when the absorbable suture was degraded for 21 days, and the results and the corresponding parameters of each example are shown in table 5.
TABLE 5 corresponding parameters of examples 5-10 and the inhibition rate of the prepared suture after 21 days degradation
As can be seen from table 5, changing the mass fractions of acetic acid and urea in the mixed solution in step S1 and the mass ratio of chitosan to the mixed solution have a certain effect on the antibacterial performance of the produced absorbable suture thread for obstetrics and gynecology department. Comparing the example 1 with the examples 5-8, it can be seen that when the mass ratio of the chitosan to the mixed solution is kept unchanged, the bacteriostasis rate of the prepared suture line is increased along with the increase of the content of acetic acid or urea; but excessive acetic acid or urea can affect the degradation condition and biocompatibility of the suture. Therefore, the mass fraction of acetic acid in the mixed solution is preferably 4-7%, and the mass fraction of urea is preferably 1-3%.
Comparing example 1 with examples 9-10, it can be seen that when the content of acetic acid and urea in the mixed solution is kept unchanged, the antibacterial rate of the prepared suture increases with the increase of the mass ratio of chitosan to the mixed solution, but the later-stage increase is not large, which indicates that the chitosan basically reaches a saturated state in the solution, and the antibacterial effect of the suture is not greatly improved by continuously increasing the content of chitosan. Therefore, the mass ratio of the chitosan to the mixed solution is preferably 1 (15-25).
Examples 11 to 13
Examples 11 to 13 each provide a method for producing an absorbable suture thread for obstetrics and gynecology department, which is different from example 1 in that the mass ratio of the lactic acid solution to the polyethylene glycol solution and the mass ratio of the polylactic acid to the chitosan in step S2 are changed, and the mass ratios of the examples are shown in table 6.
TABLE 6 Mass ratios of lactic acid to polyethylene glycol and polylactic acid to chitosan in examples 11 to 13
The mechanical properties, antibacterial properties and degradation properties of the absorbable suture for obstetrics and gynecology prepared in the examples were measured, and the results are shown in table 7.
TABLE 7 Performance parameters of the absorbable suture for obstetrics and gynecology department prepared in examples 11 to 13
As can be seen from table 7, the change of the mass ratio of the lactic acid solution to the polyethylene glycol solution and the mass ratio of the polylactic acid to the chitosan in step S2 has a certain effect on the mechanical properties, antibacterial properties and degradation properties of the absorbable suture thread for obstetrics and gynecology department. Comparing example 1 with examples 11-12, it can be seen that when the mass ratio of polylactic acid to chitosan is constant, the breaking strength and breaking elongation of the prepared suture line tend to increase and then decrease with the increase of the mass ratio of the lactic acid solution to the polyethylene glycol solution; mainly because the content of the prepared polylactic acid is relatively low when the content of the lactic acid is low, and the content of the polyethylene glycol is relatively low when the content of the lactic acid is high, the activity of the prepared polylactic acid end group is insufficient, so that the grafting rate of the chitosan is relatively low, and the mechanical property of the suture is influenced. Therefore, the mass ratio of the lactic acid solution to the polyethylene glycol solution is preferably (0.8-1.2): 1.
Comparing example 1 with example 13, it can be seen that when the mass ratio of the lactic acid solution to the polyethylene glycol solution is constant, the mechanical properties of the prepared suture thread are enhanced with the increase of the mass ratio of the polylactic acid to the chitosan, but the antibacterial property and the degradation property are weakened. Therefore, the mass ratio of the polylactic acid to the chitosan is preferably (12-15): 1, in combination with various properties of the suture.
Comparative example 6
This comparative example provides a method for preparing an absorbable suture thread for obstetrics and gynecology department, which is different from example 1 in that the sequence of modification and melt spinning of polylactic acid in step S2 is changed. The comparative example is prepared by preparing a polylactic acid thread, then immersing the polylactic acid thread into a solution containing chitosan for modification, and drying to obtain the chitosan modified polylactic acid thread. The content of each raw material in the comparative example is consistent with that in example 1, and the test results of the mechanical property, antibacterial property and degradation property of the prepared absorbable suture for obstetrics and gynecology department are shown in table 8.
TABLE 8 Performance parameters of the absorbable suture thread for obstetrics and gynecology department prepared in comparative example 6
As can be seen from Table 8, the absorbable suture for obstetrics and gynecology department prepared in comparative example 6 has relatively poor comprehensive properties as compared with example 1. The main reason is that the polylactic acid silk thread is difficult to fully react by impregnation modification after being prepared, and the internal modification rate of the polylactic acid silk thread is low, so that the obvious modification effect is difficult to achieve.
In conclusion, the absorbable suture line for obstetrics and gynecology department prepared by the invention has better mechanical property, antibacterial property and degradation property, can provide enough support before the wound of a patient is recovered, has the effect of long-acting bacteriostasis, and simultaneously promotes the inward growth of the damaged tissues around the suture line to achieve the effect of accelerating healing; and the absorbable suture line has simple integral preparation process and convenient control, and can meet the requirements of actual production and application.
The above description is only for the purpose of illustrating the technical solutions of the present invention and is not intended to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; all the equivalent structures or equivalent processes performed by using the contents of the specification and the drawings of the invention, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. An absorbable suture line for obstetrics and gynecology department, which is characterized by comprising a core wire and a multi-strand shell wire braided around the core wire, wherein the outer side of the multi-strand shell wire is coated with a coating; the core wire is a chitosan wire, the shell wire is a modified polylactic acid wire, and the coating comprises calcium stearate and polylactic acid; the absorbable suture for obstetrics and gynecology department is prepared by the following steps:
s1, dissolving chitosan in a mixed solution containing acetic acid and urea, filtering and degassing to obtain a spinning solution, and preparing a chitosan yarn by wet spinning;
s2, mixing the lactic acid solution with the polyethylene glycol solution, preparing polylactic acid by adopting a solution polycondensation method, mixing and heating the prepared polylactic acid and chitosan to prepare chitosan grafted modified polylactic acid, and preparing modified polylactic acid threads by adopting a melt spinning method;
s3, taking the chitosan silk thread obtained in the step S1 as a core thread, taking the modified polylactic acid silk thread obtained in the step S2 as a shell thread, and performing stranding knitting by adopting a knitting machine to obtain a knitting thread;
s4, mixing calcium stearate and polylactic acid, and dissolving in absolute ethyl alcohol to prepare a coating solution; and soaking the braided wire prepared in the step S3 into the coating liquid, taking out and drying after fully soaking, and preparing the absorbable suture for obstetrics and gynecology department.
2. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: the diameter of the core wire is 0.1-0.2 mm, and the diameter of the shell wire is 0.06-0.2 mm.
3. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: the number of strands of the multi-strand shell line is 6-8 strands.
4. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: the deacetylation degree of chitosan in the chitosan silk thread is 90%, and the modified polylactic acid is chitosan grafted modified polylactic acid.
5. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: the mass ratio of calcium stearate to polylactic acid in the coating is 1: 1.
6. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: in step S1, the mass fraction of acetic acid in the mixed solution is 4% -7%, the mass fraction of urea is 1% -3%, and the balance is water.
7. An absorbable suture for obstetrics and gynecology department according to claim 6, characterized in that: in step S1, the mass ratio of the chitosan to the mixed solution is 1 (15-25).
8. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: in step S2, the concentration of the lactic acid solution is 88%, the concentration of the polyethylene glycol solution is 50%, and the mass ratio of the lactic acid solution to the polyethylene glycol solution is (0.8-1.2): 1.
9. An absorbable suture for obstetrics and gynecology department according to claim 1, characterized in that: in step S2, the mass ratio of polylactic acid to chitosan is (12-15): 1.
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| CN115287904A (en) * | 2022-10-10 | 2022-11-04 | 江苏恒力化纤股份有限公司 | Preparation method of absorbable medical suture |
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| CN103215779B (en) * | 2013-04-08 | 2015-04-08 | 东华大学 | Device and method for preparing sustainable-released antibacterial real-silk braided suture line |
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