CN110664655B

CN110664655B - Cell culture solution

Info

Publication number: CN110664655B
Application number: CN201910893323.0A
Authority: CN
Inventors: 姜婷
Original assignee: Xi'an Bohe Medical Technology Co ltd
Current assignee: Xi'an Bohe Medical Technology Co ltd
Priority date: 2019-09-20
Filing date: 2019-09-20
Publication date: 2023-02-28
Anticipated expiration: 2039-09-20
Also published as: CN110664655A

Abstract

The invention provides a cell culture solution, wherein the cell culture solution comprises inorganic salt, vitamins, amino acids, ribonucleosides and coenzyme. The invention also provides a whitening composition containing the cell culture solution. The whitening composition disclosed by the invention is better in stability and can obviously increase the skin brightness.

Description

Cell culture solution

Technical Field

The invention relates to the field of skin care products, and particularly relates to a cell culture solution.

Background

With the progress of society and the improvement of living standard of people, the beauty skin care product becomes an indispensable beauty element in life, and the green and natural beauty skin care product also becomes an exploration theme of the skin care product. However, there are a lot of messy whitening products in the market, and some manufacturers add a large amount of chemicals harmful to the human body into skin care products in order to achieve the effects of whitening and repairing the skin, and even add limited or forbidden chemicals containing drugs, hormones and the like into skin care products, and the long-term use of the harmful substances can cause serious damage to the human body.

The rapid development of the beauty industry brings good news to the masses of people who love beauty. However, due to the early non-standardization and urgent work and near-profit of the industry in the sun, some inferior products, even quick-acting products with side effects on skin, flow into the market, and cause deep or shallow injuries to some beauty lovers. Moreover, the environmental problems of food pollution, air pollution and the like in the current society also affect the health beauty of human beings to different degrees, so that people have the tendency of increasing and accumulating toxins no matter eating internally or using externally applied cosmetics, thereby leading to the skin to be dark.

Patent CN103860444a discloses a whitening essence containing siRNA cell culture for inhibiting melanin production, which is prepared from epidermal cell culture solution, contains siRNA for inhibiting tyrosinase (Tyr) expression, and blocks melanin formation in skin tissue melanocytes, but CN103860444a has whitening efficacy lacking human body data support and has no stability data.

Therefore, in the field of skin care products, there is a strong demand for a whitening composition having better stability and capable of significantly increasing skin brightness.

Disclosure of Invention

In view of the above, the present invention aims to provide a whitening composition having better stability and capable of significantly increasing skin brightness.

Therefore, the invention provides the following technical scheme.

In a first aspect, the invention provides a cell culture fluid, wherein the cell culture fluid comprises inorganic salts, vitamins, amino acids, ribonucleosides and coenzymes.

In a preferred embodiment, the inorganic salt is selected from one or more of potassium chloride, sodium chloride, calcium chloride, sodium bicarbonate, magnesium sulfate and disodium hydrogen phosphate.

In a more preferred embodiment, the potassium chloride may be present in an amount of 10 parts by weight ^-3 -1; for example, the potassium chloride may be present in an amount of 10 parts by weight ^-3 -0.5。

In a more preferred embodiment, the sodium chloride may be present in an amount of 10 parts by weight ^-3 -1; for example, the sodium chloride may be present in an amount of 10 parts by weight ^-3 -0.5。

In a more preferred embodiment, the calcium chloride may be present in an amount of 10 parts by weight ^-3 -1; for example, the calcium chloride may be present in an amount of 1 part by weight0 ^-3 -0.5。

In a more preferred embodiment, the sodium bicarbonate may be present in an amount of 10 parts by weight ^-3 -1; for example, the sodium bicarbonate can be present in an amount of 10 parts by weight ^-3 -0.5。

In a more preferred embodiment, the magnesium sulfate may be 10 parts by weight ^-3 -1; for example, the magnesium sulfate may be 10 parts by weight ^-3 -0.5。

In a more preferred embodiment, the disodium phosphate may be present in an amount of 10 parts by weight ^-3 -1; for example, the disodium phosphate may be present in an amount of 10 parts by weight ^-3 -0.5。

In a preferred embodiment, the vitamin is selected from one or more of vitamin B2, vitamin B6, vitamin B7, vitamin B9, vitamin B12 and vitamin E.

In a more preferred embodiment, the vitamin B2 may be present in an amount of 10 parts by weight ^-6 -1; for example, the vitamin B2 may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the vitamin B6 may be present in an amount of 10 parts by weight ^-6 -1; for example, the vitamin B6 may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the vitamin B7 may be present in an amount of 10 parts by weight ^-6 -1; for example, the vitamin B7 may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the vitamin B9 may be present in an amount of 10 parts by weight ^-6 -1; for example, the vitamin B9 may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the vitamin B12 may be present in an amount of 10 parts by weight ^-6 -1; for example, the vitamin B12 may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the vitamin E may be present in an amount of 10 parts by weight ^-6 -1; for example, the vitamin E may be present in an amount of 10 parts by weight ^-6 -0.01。

In a preferred embodiment, the amino acid is selected from one or more of lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine and phenylalanine.

In a more preferred embodiment, the lysine may be present in an amount of 10 parts by weight ^-6 -1; for example, the lysine may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the histidine may be present in an amount of 10 parts by weight ^-6 -1; for example, the histidine may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the arginine may be present in an amount of 10 parts by weight ^-6 -1; for example, the arginine may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the aspartic acid may be present in an amount of 10 parts by weight ^-6 -1; for example, the aspartic acid may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the weight part of threonine may be 10 ^-6 -1; for example, the threonine can be 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the serine may be present in an amount of 10 parts by weight ^-6 -1; for example, the serine can be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the glutamic acid may be 10 parts by weight ^-6 -1; for example, the glutamic acid may be 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the proline may be present in an amount of 10 parts by weight ^-6 -1; for example, the proline may be present in 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the glycine may be present in an amount of 10 parts by weight ^-6 -1; for example, the weight of glycineThe amount of the component can be 10 ^-6 -0.01。

In a more preferred embodiment, the alanine may be present in an amount of 10 parts by weight ^-6 -1; for example, the alanine can be present in 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the valine can be present in an amount of 10 parts by weight ^-6 -1; for example, the valine can be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the isoleucine may be present in an amount of 10 parts by weight ^-6 -1; for example, the isoleucine can be 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the leucine may be present in an amount of 10 parts by weight ^-6 -1; for example, the leucine may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the tyrosine may be present in an amount of 10 parts by weight ^-6 -1; for example, the tyrosine may be present in an amount of 10 parts by weight ^-6 -0.01。

In a more preferred embodiment, the phenylalanine may be present in an amount of 10 parts by weight ^-6 -1; for example, the phenylalanine may be present in an amount of 10 parts by weight ^-6 -0.01。

In a preferred embodiment, the ribonucleoside is selected from one or more of 2' -deoxyadenosine, 2' -deoxycytidine hydrochloride, 2' -deoxyguanosine and thymidine.

In a more preferred embodiment, the 2' -deoxyadenosine may be present in 10 parts by weight ^-8 -1; for example, the 2' -deoxyadenosine can be 10 parts by weight ^-8 -0.005。

In a more preferred embodiment, the 2' -deoxycytidine hydrochloride may be present in an amount of 10 parts by weight ^-8 -1; for example, the 2' -deoxycytidine hydrochloride may be 10 parts by weight ^-8 -0.005。

In a more preferred embodiment, the 2' -deoxyguanosine may be present in a weight fraction of 10 ^-8 -1; for example, the 2' -deoxyguanosine may be present in parts by weightIs 10 ^-8 -0.005。

In a more preferred embodiment, the thymidine may be 10 parts by weight ^-8 -1; for example, the thymidine may be 10 parts by weight ^-8 -0.005。

In a preferred embodiment, the coenzyme is selected from one or more of coenzyme a, coenzyme I, flavin adenine dinucleotide, pyridine nucleotide triphosphate-sodium, cocarboxylase, and uracil nucleoside triphosphate.

In a more preferred embodiment, the coenzyme A may be present in an amount of 10 parts by weight ^-8 -1; for example, the coenzyme A may be present in an amount of 10 parts by weight ^-8 -0.005。

In a more preferred embodiment, the coenzyme I may be present in an amount of 10 parts by weight ^-8 -1; for example, the coenzyme I may be present in an amount of 10 parts by weight ^-8 -0.005。

In a more preferred embodiment, the flavin adenine dinucleotide may be present in an amount of 10 parts by weight ^-8 -1; for example, the flavin adenine dinucleotide may be present in an amount of 10 parts by weight ^-8 -0.005。

In a more preferred embodiment, the pyridine nucleotide triphosphate-sodium may be present in an amount of 10 parts by weight ^-8 -1; for example, the pyridine nucleotide triphosphate-sodium can be present in a weight fraction of 10 ^-8 -0.005。

In a more preferred embodiment, the cocarboxylase may be present in an amount of 10 parts by weight ^-8 -1; for example, the cocarboxylase may be present in an amount of 10 parts by weight ^-8 -0.005。

In a more preferred embodiment, the number of parts by weight of the uracil nucleoside triphosphate may be 10 ^-8 -1; for example, the uracil nucleoside triphosphate can be present in 10 parts by weight ^-8 -0.005。

In a second aspect, a whitening composition is provided, wherein the whitening composition comprises the cell culture fluid of the invention.

In a preferred embodiment, the cell culture fluid is 0.1 to 90 parts by weight; for example, the cell culture fluid can be 0.1, 0.5, 1, 2, 5, 8, 10, 15, 20, 22, 26, 30, 33, 36, 40, 43, 49, 50, 55, 60, 62, 67, 70, 75, 80, 82, 86, or 90 parts by weight.

In a preferred embodiment, the whitening composition further comprises a whitening ingredient selected from one or more of vitamin C, glutathione, niacinamide, lipoic acid, tranexamic acid and arbutin.

In a more preferred embodiment, the whitening ingredient is present in an amount of 0.1 to 20 parts by weight; for example, the whitening ingredient may be present in an amount of 0.1, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 parts by weight.

In a more preferred embodiment, the vitamin C may be present in an amount of 0.01 to 20 parts by weight; for example, the vitamin C may be present in an amount of 0.01 to 15 parts by weight.

In a more preferred embodiment, the weight fraction of glutathione may be 0.01 to 15; for example, the weight portion of the glutathione may be 0.01 to 10.

In a more preferred embodiment, the weight fraction of nicotinamide may be 0.1-20; for example, the nicotinamide may be present in an amount of 0.1 to 15 parts by weight.

In a more preferred embodiment, the lipoic acid may be present in an amount of 0.01 to 20 parts by weight; for example, the lipoic acid may be present in an amount of 0.01 to 10 parts by weight.

In a more preferred embodiment, the lipoic acid may be alpha-lipoic acid.

In a more preferred embodiment, the tranexamic acid may be present in an amount of 0.01 to 20 parts by weight; for example, the tranexamic acid may be present in an amount of 0.01 to 15 parts by weight.

In a more preferred embodiment, the arbutin may be present in an amount of 0.01 to 15 parts by weight; for example, the arbutin may be present in an amount of 0.01 to 10 parts by weight.

In a preferred embodiment, the whitening composition further comprises a repair ingredient selected from one or more of ceramide, tetrahydromethylpyrimidine carboxylic acid and allantoin.

In a more preferred embodiment, the repair component is present in an amount of 0.1 to 30 parts by weight; for example, the repair component may be present in a weight fraction of 0.1, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30.

In a more preferred embodiment, the ceramide may be present in an amount of 0.01 to 30 parts by weight; for example, the ceramide may be present in an amount of 0.01 to 20 parts by weight.

In a more preferred embodiment, the part by weight of the tetrahydro-methyl-pyrimidine-carboxylic acid may be from 0.01 to 15; for example, the weight fraction of the tetrahydromethylpyrimidine carboxylic acid may be from 0.01 to 10.

In a more preferred embodiment, the allantoin may be in a weight fraction of 0.01 to 15; for example, the allantoin may be in an amount of 0.01 to 10 parts by weight.

In a preferred embodiment, the whitening composition further comprises a moisturizing ingredient selected from one or more of sodium hyaluronate, hydrolyzed sodium hyaluronate, pentanediol, butanediol, and hydroxyethyl urea.

In a more preferred embodiment, the moisture retaining component is present in an amount of 0.1 to 70 parts by weight; for example, the moisturizing ingredient can be present in 0.1, 0.5, 1, 2, 3, 5, 6, 8, 10, 12, 15, 20, 22, 26, 28, 30, 33, 35, 36, 38, 40, 43, 46, 49, 50, 52, 55, 58, 60, 62, 65, 67, 69, or 70 parts by weight.

In a more preferred embodiment, the sodium hyaluronate may be present in an amount of 0.001 to 10 parts by weight; for example, the sodium hyaluronate may be present in an amount of 0.001 to 5 parts by weight.

In a more preferred embodiment, the hydrolyzed sodium hyaluronate may be present in an amount of 0.001 to 15 parts by weight; for example, the hydrolyzed sodium hyaluronate may be present in an amount of 0.001 to 10 parts by weight.

In a more preferred embodiment, the weight fraction of the pentanediol may be from 0.1 to 20; for example, the parts by weight of the pentanediol may be 0.1 to 15 or 0.1 to 10.

In a more preferred embodiment, the weight fraction of the butanediol may be in the range of 0.1 to 50; for example, the butanediol may be present in an amount of 0.1 to 30 or 0.1 to 10 parts by weight.

In a more preferred embodiment, the hydroxyethyl urea may be present in an amount of 0.1 to 80 parts by weight; for example, the hydroxyethyl urea can be present in an amount of 0.1 to 50 or 0.1 to 20 parts by weight.

In a third aspect, the present invention provides a method for preparing a whitening composition, the method comprising the steps of:

s1, adding cell nutrient solution components, moisture-preserving components and water into a container, uniformly mixing, heating to 75-85 ℃, and preserving heat for 20-30min until complete dissolution to obtain a mixture A;

s2, cooling the mixture A to 40-45 ℃ in a water bath, adding the whitening component and the repairing component, uniformly stirring at 50-60rpm, and discharging at 33-38 ℃;

s3, immediately filtering the mixture by using a 0.22-micron filter membrane after discharging, and then carrying out aseptic filling and nitrogen filling.

In a fourth aspect, a whitening product is provided, wherein the whitening product comprises the cell culture solution or the whitening composition of the invention.

In addition, the invention provides application of the cell culture solution in preparation of whitening products.

And provides the application of the whitening composition in preparing whitening products.

Compared with the prior art, the invention has the beneficial effects that:

the whitening composition prepared by matching the cell culture solution with the whitening component, the repairing component and the moisturizing component is relatively mild and has better moisturizing property, and meanwhile, the skin brightness can be obviously increased.

The cell culture solution disclosed by the invention can provide energy and nutrient substances for cells, improve the activity of skin cells, delay cell aging, enable the skin cells to be more robust, resist external stimulus factors, delay cell aging and promote the young state of the skin.

The whitening component of the invention can obviously increase the skin brightness. Controlling the weight part of the vitamin C in the whitening component within the range of 0.01-20 can better inhibit the activity of tyrosinase through the antioxidant property of the vitamin C, and is helpful for the reduction of dopa pigment (tyrosine is converted into an intermediate of melanin), so that the generation of melanin is reduced and inhibited, and the action of peroxidase can be inhibited; the glutathione in the whitening component is controlled within the range of 0.01 to 15 by weight parts, so that various toxic compounds, heavy metal ions and carcinogenic substances can be better chelated; the weight part of the nicotinamide in the whitening component is controlled within the range of 0.1-20, so that the transfer of melanin to keratinocytes can be effectively inhibited, and when part of melanin inevitably reaches epidermal skin, the nicotinamide can accelerate the renewal speed of skin cells so as to promote the cells containing the melanin to fall off; the weight portion of the alpha-lipoic acid in the whitening component is controlled within the range of 0.01-20, so that the enzyme activity of the glutathione reductase can be better improved from the expression level of genes and proteins through a redox sensitive transcription factor 2 or an antioxidant response element signal path, the regeneration of reduced glutathione is regulated and controlled, and the antioxidant and whitening capabilities of the glutathione are greatly improved; the monophenolase enzyme activity can be better inhibited by controlling the weight part of arbutin in the whitening component within the range of 0.01-15, and the tyrosinase is prevented from catalyzing L tyrosine to be converted into L-dopa; the whitening and spot-lightening effect is better when the weight part of tranexamic acid in the whitening component is controlled within the range of 0.01-20.

The repairing component of the invention can ensure that the skin can be best repaired. The weight portion of the tetrahydro-methyl pyrimidine carboxylic acid in the repairing component is controlled within the range of 0.01-15, the weight portion of the ceramide in the repairing component is controlled within the range of 0.01-30, and the weight portion of the allantoin in the repairing component is controlled within the range of 0.01-15, so that the three substances have a synergistic skin repairing effect, can better resist external damage, promote cell repair and enhance the immune function of skin cells.

The moisturizing component can remarkably improve the skin moisturizing property.

In addition, the whitening composition can keep excellent stability at low temperature (about-18 ℃) to high temperature (45 ℃), has no delamination or precipitation and has good stability.

Detailed Description

The present invention will be specifically explained below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly demonstrated. It will be understood by those skilled in the art that these specific embodiments and examples are illustrative of the invention and are not to be construed as limiting the invention.

The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

Example 1

The present embodiment provides a whitening composition comprising a cell broth ingredient comprising an inorganic salt selected from the group consisting of vitamin B2, vitamin B6, vitamin B7, vitamin B9, vitamin B12 and vitamin E, a vitamin selected from the group consisting of lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine and phenylalanine, an amino acid selected from the group consisting of lysine, histidine, arginine, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine and phenylalanine, a coenzyme selected from the group consisting of 2' -deoxyadenosine, 2' -deoxycytidine hydrochloride, 2' -deoxyguanosine and thymidine, a whitening ingredient selected from the group consisting of vitamin C, glutathione, nicotinamide, lipoic acid, tranexamic acid and arbutin, a repair ingredient selected from the group consisting of neuropeptide-sodium triphosphate, cocarboxylase and uracil nucleoside, and a moisturizing ingredient selected from the group consisting of glycofurosemide, glycofurol, hyaluronic acid and a moisturizing ingredient selected from the group consisting of vitamin C, glutathione, nicotinamide, lipoic acid, sodium hyaluronate, and hydrolyzed hydroxypivalate,

the weight portion of the potassium chloride is 10 ^-3 The weight portion of the sodium chloride is 10 ^-3 Said chlorineThe weight portion of the calcium is 10 ^-3 The weight portion of the sodium bicarbonate is 10 ^-3 The weight part of the magnesium sulfate is 10 ^-3 The weight portion of the disodium hydrogen phosphate is 10 ^-3 The weight part of the vitamin B2 is 10 ^-6 The weight portion of the vitamin B6 is 10 ^-6 The weight portion of the vitamin B7 is 10 ^-6 The weight portion of the vitamin B9 is 10 ^-6 The weight portion of the vitamin B12 is 10 ^-6 The weight portion of the vitamin E is 10 ^-6 The weight portion of the lysine is 10 ^-6 The weight part of the histidine is 10 ^-6 The weight portion of the arginine is 10 ^-6 The weight portion of the aspartic acid is 10 ^-6 The weight portion of the threonine is 10 ^-6 The weight portion of the serine is 10 ^-6 The weight portion of the glutamic acid is 10 ^-6 The weight part of the proline is 10 ^-6 The weight portion of the glycine is 10 ^-6 The weight portion of the alanine is 10 ^-6 The weight portion of the valine is 10 ^-6 The weight part of the isoleucine is 10 ^-6 The weight portion of the leucine is 10 ^-6 The weight portion of the tyrosine is 10 ^-6 The weight portion of the phenylalanine is 10 ^-6 The weight portion of the 2' -deoxyadenosine is 10 ^-8 The weight portion of the 2' -deoxycytidine hydrochloric acid is 10 ^-8 The weight portion of the 2' -deoxyguanosine is 10 ^-8 The weight portion of the thymidine is 10 ^-8 The weight portion of the coenzyme A is 10 ^-8 The weight portion of the coenzyme I is 10 ^-8 The weight portion of the flavin adenine dinucleotide is 10 ^-8 The weight portion of the pyridine nucleotide triphosphate-sodium is 10 ^-8 The weight portion of the cocarboxylase is 10 ^-8 The weight portion of the uracil nucleoside triphosphate is 10 ^-8 ；

The vitamin C is 0.01 part by weight, the glutathione is 0.01 part by weight, the nicotinamide is 0.1 part by weight, the lipoic acid is 0.01 part by weight, the tranexamic acid is 0.01 part by weight, and the arbutin is 0.01 part by weight;

the weight portion of the ceramide is 0.01, the weight portion of the tetrahydro-methyl pyrimidine carboxylic acid is 0.01, and the weight portion of the allantoin is 0.01;

the weight portion of the sodium hyaluronate is 0.001, the weight portion of the hydrolyzed sodium hyaluronate is 0.001, the weight portion of the pentanediol is 0.1, the weight portion of the butanediol is 0.1, and the weight portion of the hydroxyethyl urea is 0.1.

The whitening composition of this example was prepared as follows:

s1, adding 10 ^-3 Potassium chloride 10 parts ^-3 Sodium chloride, 10 ^-3 Calcium chloride, 10 ^-3 Sodium bicarbonate, 10 ^-3 Magnesium sulfate portion, 10 ^-3 Sodium phosphate dibasic, 10 ^-6 Vitamin B2, 10 ^-6 Vitamin B6, 10 ^-6 Vitamin B7, 10 ^-6 Vitamin B9, vitamin B10 ^-6 Vitamin B12, 10 ^-6 Vitamin E, 10 ^-6 Lysine portion 10 ^-6 Histidine share, 10 ^-6 Arginine, 10 parts ^-6 Aspartic acid, 10 ^-6 Threonine fraction 10 ^-6 Serine, 10 ^-6 Glutamic acid portion, 10 ^-6 Proline fraction 10 ^-6 Glycine portion 10 ^-6 Alanine, 10 ^-6 Fractional valine, 10 ^-6 Portions of isoleucine, 10 ^-6 Fraction leucine, 10 ^-6 Tyrosine, 10 ^-6 Phenylalanine, 10 ^-8 2' -deoxyadenosine, 10 ^-8 2' -deoxycytidine hydrochloride, 10 ^-8 2' -deoxyguanosine, 10 ^-8 Thymidine, 10 ^-8 Coenzyme A, 10 ^-8 Coenzyme I, 10 ^-8 Perflavin adenine dinucleotide, 10 ^-8 10 parts of pyridine nucleotide triphosphate-sodium ^-8 Cocarboxylase, 10 ^-8 Adding the parts by weight of uracil nucleoside triphosphate, 0.001 part of sodium hyaluronate, 0.001 part of hydrolyzed sodium hyaluronate, 0.1 part of pentanediol, 0.1 part of butanediol, 0.1 part of hydroxyethyl urea and a proper amount of water into a container, uniformly mixing,heating to 75 ℃, and preserving heat for 20min until the mixture is completely dissolved to obtain a mixture A;

s2, cooling the mixture A to 40 ℃ in a water bath, adding 0.01 part of vitamin C, 0.01 part of glutathione, 0.1 part of nicotinamide, 0.01 part of lipoic acid, 0.01 part of tranexamic acid, 0.01 part of arbutin, 0.01 part of ceramide, 0.01 part of tetrahydro-methyl pyrimidine carboxylic acid and 0.01 part of allantoin, uniformly stirring at 50rpm, and discharging at 33 ℃;

Example 2

the weight portion of the potassium chloride is 1, the weight portion of the sodium chloride is 1, the weight portion of the calcium chloride is 1, the weight portion of the sodium bicarbonate is 1, the weight portion of the magnesium sulfate is 1, the weight portion of the disodium hydrogen phosphate is 1, the weight portion of the vitamin B2 is 1, the weight portion of the vitamin B6 is 1, the weight portion of the vitamin B7 is 1, the weight portion of the vitamin B9 is 1, the weight portion of the vitamin B12 is 1, the weight portion of the vitamin E is 1, the weight portion of the lysine is 1, the weight portion of the histidine is 1, the weight portion of the arginine is 1, the weight portion of the aspartic acid is 1, the weight portion of the threonine is 1, the weight portion of the serine is 1, the weight portion of the glutamic acid is 1, the weight portion of the proline is 1, the weight portion of the glycine is 1, the weight portion of the alanine is 1, the weight portion of the valine is 1, the weight portion of the isoleucine is 1, the weight portion of the leucine is 1, the weight portion of the tyrosine is 1, the weight portion of the phenylalanine is 1, the weight portion of the 2' -deoxyadenosine is 1, the weight portion of the 2' -deoxycytidine hydrochloride is 1, the weight portion of the 2' -deoxyguanosine is 1, the weight portion of the thymidine is 1, the weight portion of the coenzyme A is 1, the weight portion of the coenzyme I is 1, the weight portion of the flavin adenine dinucleotide is 1, the weight portion of the pyridine triphosphate nucleotide-sodium is 1, and the weight portion of the cocarboxylase is 1, the weight part of the uracil nucleoside triphosphate is 1;

the vitamin C is 20 parts by weight, the glutathione is 15 parts by weight, the nicotinamide is 20 parts by weight, the lipoic acid is 20 parts by weight, the tranexamic acid is 20 parts by weight, and the arbutin is 15 parts by weight;

the weight portion of the ceramide is 30, the weight portion of the tetrahydro-methyl pyrimidine carboxylic acid is 15, and the weight portion of the allantoin is 15;

the weight portion of the sodium hyaluronate is 10, the weight portion of the hydrolyzed sodium hyaluronate is 15, the weight portion of the pentanediol is 20, the weight portion of the butanediol is 50, and the weight portion of the hydroxyethyl urea is 80.

The whitening composition of this example was prepared as follows:

s1, dissolving 1 part of potassium chloride, 1 part of sodium chloride, 1 part of calcium chloride, 1 part of sodium bicarbonate, 1 part of magnesium sulfate, 1 part of disodium hydrogen phosphate, 1 part of vitamin B2, 1 part of vitamin B6, 1 part of vitamin B7, 1 part of vitamin B9, 1 part of vitamin B12, 1 part of vitamin E, 1 part of lysine, 1 part of histidine, 1 part of arginine, 1 part of aspartic acid, 1 part of threonine, 1 part of serine, 1 part of glutamic acid, 1 part of proline, 1 part of glycine, 11 parts of alanine, 1 part of valine, 1 part of isoleucine, 1 part of leucine, 1 part of tyrosine, 1 part of phenylalanine, 1 part of 2' -deoxyadenosine, 1 part of 2' -deoxycytidine hydrochloride, 1 part of 2' -deoxyguanosine, 1 part of thymidine, 1 part of coenzyme A, 1 part of coenzyme I, 1 part of flavin adenine dinucleotide, 1 part of pyridine nucleotide-sodium triphosphate, 1 part of cocarboxylase, 1 part of urea riboside, 10 parts of sodium hyaluronate, 15 parts of water, 20 parts of sodium glutarate, and water in a container at a temperature of 80 ℃ to obtain a mixture, and heating the mixture to a temperature of water uniformly;

s2, cooling the mixture A to 45 ℃ in a water bath, adding 20 parts of vitamin C, 15 parts of glutathione, 20 parts of nicotinamide, 20 parts of lipoic acid, 20 parts of tranexamic acid, 15 parts of arbutin, 30 parts of ceramide, 15 parts of tetrahydro-methylpyrimidine carboxylic acid and 15 parts of allantoin, uniformly stirring at 60rpm, and discharging at 38 ℃;

and S3, immediately filtering the mixture by using a 0.22-micron filter membrane after discharging, and then performing aseptic filling by filling nitrogen.

Example 3

The present embodiment provides a whitening composition comprising a cell broth ingredient comprising an inorganic salt selected from the group consisting of potassium chloride, sodium chloride, calcium chloride, sodium bicarbonate, magnesium sulfate and disodium hydrogen phosphate, a vitamin selected from the group consisting of vitamin B2, vitamin B6, vitamin B7, vitamin B9, vitamin B12 and vitamin E, a whitening ingredient selected from the group consisting of potassium chloride, sodium chloride, calcium chloride, sodium bicarbonate, magnesium sulfate and disodium hydrogen phosphate, a repair ingredient selected from the group consisting of a cell broth ingredient selected from the group consisting of vitamin C, glutathione, nicotinamide, lipoic acid, tranexamic acid, flavine adenine dinucleotide, sodium pyridine triphosphate, cocarboxylase and uracil nucleoside triphosphate, a moisturizing ingredient selected from the group consisting of vitamin B2 ' -deoxyadenosine, 2' -deoxycytidine hydrochloride, 2' -deoxyguanosine hydrochloride and thymidine, a moisturizing ingredient selected from the group consisting of coenzyme a, coenzyme I, flavin adenine dinucleotide, sodium pyridine triphosphate, cocarboxylase and uracil nucleoside triphosphate, a whitening ingredient selected from the group consisting of vitamin C, glutathione, nicotinamide, lipoic acid, tranexamine and arbutin, a repair ingredient selected from the group consisting of tetrahydropyrimidine, sodium triphosphate, glycopyrrolate, sodium hyaluronate, hydrolyzed hydroxypivalate, and hydrolyzed sodium hyaluronate,

the weight portion of the potassium chloride is 0.5, the weight portion of the sodium chloride is 0.5, the weight portion of the calcium chloride is 0.5, the weight portion of the sodium bicarbonate is 0.5, the weight portion of the magnesium sulfate is 0.5, the weight portion of the disodium hydrogen phosphate is 0.5, the weight portion of the vitamin B2 is 0.01, the weight portion of the vitamin B6 is 0.01, the weight portion of the vitamin B7 is 0.01, the weight portion of the vitamin B9 is 0.01, the weight portion of the vitamin B12 is 0.01, the weight portion of the vitamin E is 0.01, the weight portion of the lysine is 0.01, the weight portion of the histidine is 0.01, the weight portion of the arginine is 0.01, the weight portion of the aspartic acid is 0.01, the weight portion of the threonine is 0.01, the weight portion of the serine is 0.01, the weight portion of the glutamic acid is 0.01, the weight portion of the proline is 0.01, the weight portion of the glycine is 0.01, the weight portion of the alanine is 0.01, the weight portion of the valine is 0.01, the weight portion of the isoleucine is 0.01, the weight portion of the leucine is 0.01, the weight portion of the tyrosine is 0.01, the weight portion of the phenylalanine is 0.01, the weight portion of the 2' -deoxyadenosine is 0.005, the weight portion of the 2' -deoxycytidine hydrochloride is 0.005, the weight portion of the 2' -deoxyguanosine is 0.005, the weight portion of the thymidine is 0.005, the weight portion of the coenzyme A is 0.005, the weight portion of the coenzyme I is 0.005, the weight portion of the flavin adenine dinucleotide is 0.005, the weight portion of the pyridine nucleotide triphosphate-sodium is 0.005, and the weight portion of the cocarboxylase is 0.005, the weight portion of the uracil nucleoside triphosphate is 0.005;

the vitamin C is 15 parts by weight, the glutathione is 10 parts by weight, the nicotinamide is 15 parts by weight, the lipoic acid is 10 parts by weight, the tranexamic acid is 15 parts by weight, and the arbutin is 10 parts by weight;

20 parts of ceramide, 10 parts of tetrahydro-methyl pyrimidine carboxylic acid and 10 parts of allantoin;

the weight portion of the sodium hyaluronate is 5, the weight portion of the hydrolyzed sodium hyaluronate is 10, the weight portion of the pentanediol is 10, the weight portion of the butanediol is 30, and the weight portion of the hydroxyethyl urea is 20.

The whitening composition of this example was prepared as follows:

s1, 0.5 part of potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate, 0.5 part of magnesium sulfate, 0.5 part of disodium hydrogen phosphate, 0.01 part of vitamin B2, 0.01 part of vitamin B6, 0.01 part of vitamin B7, 0.01 part of vitamin B9, 0.01 part of vitamin B12, 0.01 part of vitamin E, 0.01 part of lysine, 0.01 part of histidine, 0.01 part of arginine, 0.01 part of aspartic acid, 0.01 part of threonine, 0.01 part of serine, 0.01 part of glutamic acid, 0.01 part of proline, 0.01 part of glycine, 0.01 part of alanine, 0.01 part of valine, 0.01 part of isoleucine, 0.01 part of glycine, and the like 0.01 part by weight of leucine, 0.01 part by weight of tyrosine, 0.01 part by weight of phenylalanine, 0.005 part by weight of 2' -deoxyadenosine, 0.005 part by weight of 2' -deoxycytidine hydrochloride, 0.005 part by weight of 2' -deoxyguanosine, 0.005 part by weight of thymidine, 0.005 part by weight of coenzyme A, 0.005 part by weight of coenzyme I, 0.005 part by weight of flavin adenine dinucleotide, 0.005 part by weight of pyridine nucleotide-sodium triphosphate, 0.005 part by weight of cocarboxylase, 0.005 part by weight of uracil nucleoside triphosphate, 5 parts by weight of sodium hyaluronate, 10 parts by weight of hydrolyzed sodium hyaluronate, 10 parts by weight of pentanediol, 30 parts by weight of butanediol, 20 parts by weight of hydroxyethyl urea and an appropriate amount of water are added into a container and mixed uniformly, heating to 80 deg.C, maintaining the temperature for 25min to dissolve completely to obtain mixture A;

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 10 parts of lipoic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methylpyrimidine carboxylic acid and 10 parts of allantoin, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 1

To further illustrate the beneficial effects of the present invention, comparative example 1 is provided, which is different from example 3 in that: the potassium chloride in the cell culture fluid is replaced by magnesium chloride.

The preparation method of the comparative whitening composition is as follows:

Comparative example 2

To further illustrate the beneficial effects of the present invention, comparative example 2 is provided, which comparative example 2 differs from example 3 in that: the lipoic acid in the whitening component is replaced by kojic acid.

The preparation method of the comparative whitening composition is as follows:

s1, adding 0.5 part of potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate, 0.5 part of magnesium sulfate, 0.5 part of disodium hydrogen phosphate, 0.01 part of vitamin B2, 0.01 part of vitamin B6, 0.01 part of vitamin B7, 0.01 part of vitamin B9, 0.01 part of vitamin B12, 0.01 part of vitamin E, 0.01 part of lysine, 0.01 part of histidine, 0.01 part of arginine, 0.01 part of aspartic acid, 0.01 part of threonine, 0.01 part of serine, 0.01 part of glutamic acid, 0.01 part of proline, 0.01 part of glycine, 0.01 part of alanine, 0.01 part of valine, 0.01 part of isoleucine, 0.01 part of leucine, 0.01 part of tyrosine, 0.01 part of phenylalanine, 0.005 part of deoxyadenosine, 0.005 part of 2' -deoxyuridine, 0.005 part of hydrochloric acid, 0.10 part of thymidine, 0.10 part of uridine, 0.005 part of uridine, 0.10 part of uridine phosphate, 0.005 part of sodium hyaluronate, 0.10 part of coenzyme I, 0.5 part of coenzyme A, 0.5 part of disodium phosphate, 0.005 part of coenzyme A, 0.5 part of sodium hyaluronate, 0.5 part of coenzyme, 0.5 part of uridine, 0.01 part of coenzyme A, 0.005 part of coenzyme, 0.005 part of sodium hyaluronate, 0.10 parts of deoxyadenosine, 0.005 parts of sodium hyaluronate, 0.10 parts of coenzyme, 0.005 parts of hydeoxyuridine, 0.10 parts of sodium, 0.10 parts of disodium phosphate, 0.10 parts of hydantoindoxycycline, 0.10 parts of sodium, 0.10 parts of uridine, 0.005 parts of sodium, 0.10 parts of coenzyme A, 0.01 parts of disodium phosphate, 0.005 parts of sodium hyaluronate and 10 parts of a, 0.01 parts of a, 0.005 parts of water, heating to 80 deg.C, maintaining the temperature for 25min to dissolve completely to obtain mixture A;

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 10 parts of kojic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methylpyrimidine carboxylic acid and 10 parts of allantoin, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 3

To further illustrate the beneficial effects of the present invention, comparative example 3 is provided, which comparative example 3 differs from example 3 in that: the sodium hyaluronate in the moisturizing component is replaced by glycerin.

The preparation method of the comparative whitening composition is as follows:

s1, 0.5 part of potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate, 0.5 part of magnesium sulfate, 0.5 part of disodium hydrogen phosphate, 0.01 part of vitamin B2, 0.01 part of vitamin B6, 0.01 part of vitamin B7, 0.01 part of vitamin B9, 0.01 part of vitamin B12, 0.01 part of vitamin E, 0.01 part of lysine, 0.01 part of histidine, 0.01 part of arginine, 0.01 part of aspartic acid, 0.01 part of threonine, 0.01 part of serine, 0.01 part of glutamic acid, 0.01 part of proline, 0.01 part of glycine, 0.01 part of alanine, 0.01 part of valine, 0.01 part of isoleucine, 0.01 part of glycine, and the like 0.01 part by weight of leucine, 0.01 part by weight of tyrosine, 0.01 part by weight of phenylalanine, 0.005 part by weight of 2' -deoxyadenosine, 0.005 part by weight of 2' -deoxycytidine hydrochloride, 0.005 part by weight of 2' -deoxyguanosine, 0.005 part by weight of thymidine, 0.005 part by weight of coenzyme A, 0.005 part by weight of coenzyme I, 0.005 part by weight of flavin adenine dinucleotide, 0.005 part by weight of pyridine nucleotide-sodium triphosphate, 0.005 part by weight of cocarboxylase, 0.005 part by weight of uracil nucleoside triphosphate, 5 parts by weight of glycerol, 10 parts by weight of hydrolyzed sodium hyaluronate, 10 parts by weight of pentanediol, 30 parts by weight of butanediol, 20 parts by weight of hydroxyethyl urea and an appropriate amount of water are added into a container and mixed uniformly, heating to 80 deg.C, maintaining the temperature for 25min to dissolve completely to obtain mixture A;

Comparative example 4

To further illustrate the beneficial effects of the present invention, comparative example 4 is provided, which comparative example 4 differs from example 3 in that: the allantoin in the repair component of the invention is replaced by dipotassium glycyrrhizinate.

The preparation method of the comparative whitening composition is as follows:

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 10 parts of lipoic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methyl pyrimidine carboxylic acid and 10 parts of dipotassium glycyrrhizinate, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 5

To further illustrate the beneficial effects of the present invention, comparative example 5 is provided, which comparative example 5 differs from example 3 in that: the weight part of potassium chloride in the cell culture solution is adjusted to 10 ^-4 And (4) portions are obtained.

The comparative example whitening composition was prepared as follows:

s1, adding 10 ^-4 Potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate and 0.5 part of sulfurMagnesium, 0.5 part disodium hydrogen phosphate, 0.01 part vitamin B2, 0.01 part vitamin B6, 0.01 part vitamin B7, 0.01 part vitamin B9, 0.01 part vitamin B12, 0.01 part vitamin E, 0.01 part lysine, 0.01 part histidine, 0.01 part arginine, 0.01 part aspartic acid, 0.01 part threonine, 0.01 part serine, 0.01 part glutamic acid, 0.01 part proline, 0.01 part glycine, 0.01 part alanine, 0.01 part valine, 0.01 part isoleucine, 0.01 part leucine, 0.01 part tyrosine, 0.01 part phenylalanine, 0.005 part 2' -deoxyadenosine, 0.005 part 2' -deoxycytidine hydrochloric acid, 0.005 part 2' -deoxyguanosine, 0.005 part thymidine, 0.005 part coenzyme A, 0.005 part coenzyme I, 0.005 part adenine dinucleotide, 0.005 part sodium triphosphate, 0.005-10 parts carboxypyridine triphosphate, 0.005 part sodium triphosphate, 0.005 part uracil, 10.10 parts sodium hyaluronate, 10 parts water, and heating to dissolve water homogeneously at 80 parts of water;

Comparative example 6

To further illustrate the beneficial effects of the present invention, comparative example 6 is provided, which comparative example 6 differs from example 3 in that: the weight portion of the lipoic acid in the whitening component is adjusted to 0.005.

The comparative example whitening composition was prepared as follows:

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 0.005 part of lipoic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methylpyrimidine carboxylic acid and 10 parts of allantoin, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 7

To further illustrate the beneficial effects of the present invention, comparative example 7 is provided, which comparative example 7 differs from example 3 in that: the weight portion of the sodium hyaluronate in the moisturizing component is adjusted to 0.0005 portion.

The comparative example whitening composition was prepared as follows:

s1, 0.5 part of potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate, 0.5 part of magnesium sulfate, 0.5 part of disodium hydrogen phosphate, 0.01 part of vitamin B2, 0.01 part of vitamin B6, 0.01 part of vitamin B7, 0.01 part of vitamin B9, 0.01 part of vitamin B12, 0.01 part of vitamin E, 0.01 part of lysine, 0.01 part of histidine, 0.01 part of arginine, 0.01 part of aspartic acid, 0.01 part of threonine, 0.01 part of serine, 0.01 part of glutamic acid, 0.01 part of proline, 0.01 part of glycine, 0.01 part of alanine, 0.01 part of valine, 0.01 part of isoleucine, 0.01 part of glycine, and the like 0.01 part by weight of leucine, 0.01 part by weight of tyrosine, 0.01 part by weight of phenylalanine, 0.005 part by weight of 2' -deoxyadenosine, 0.005 part by weight of 2' -deoxycytidine hydrochloride, 0.005 part by weight of 2' -deoxyguanosine, 0.005 part by weight of thymidine, 0.005 part by weight of coenzyme A, 0.005 part by weight of coenzyme I, 0.005 part by weight of flavin adenine dinucleotide, 0.005 part by weight of pyridine nucleotide-sodium triphosphate, 0.005 part by weight of cocarboxylase, 0.005 part by weight of uracil nucleoside triphosphate, 5 parts by weight of sodium hyaluronate, 0.0005 part by weight of hydrolyzed sodium hyaluronate, 10 parts by weight of pentanediol, 30 parts by weight of butanediol, 20 parts by weight of hydroxyethyl urea and an appropriate amount of water are added into a container and mixed uniformly, heating to 80 deg.C, maintaining the temperature for 25min to dissolve completely to obtain mixture A;

Comparative example 8

To further illustrate the beneficial effects of the present invention, comparative example 8 is provided, which comparative example 8 differs from example 3 in that: the weight portion of the allantoin in the repair component of the present invention was adjusted to 0.005 part.

The comparative example whitening composition was prepared as follows:

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 10 parts of lipoic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methylpyrimidine carboxylic acid and 0.005 part of allantoin, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 9

To further illustrate the beneficial effects of the present invention, comparative example 9 is provided, which comparative example 9 differs from example 3 in that: the weight part of potassium chloride in the cell culture solution is adjusted to 1.5 parts.

The comparative example whitening composition was prepared as follows:

s1, 1.5 parts of potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate, 0.5 part of magnesium sulfate, 0.5 part of disodium hydrogen phosphate, 0.01 part of vitamin B2, 0.01 part of vitamin B6, 0.01 part of vitamin B7, 0.01 part of vitamin B9, 0.01 part of vitamin B12, 0.01 part of vitamin E, 0.01 part of lysine, 0.01 part of histidine, 0.01 part of arginine, 0.01 part of aspartic acid, 0.01 part of threonine, 0.01 part of serine, 0.01 part of glutamic acid, 0.01 part of proline, 0.01 part of glycine, 0.01 part of alanine, 0.01 part of valine, 0.01 part of isoleucine 0.01 part by weight of leucine, 0.01 part by weight of tyrosine, 0.01 part by weight of phenylalanine, 0.005 part by weight of 2' -deoxyadenosine, 0.005 part by weight of 2' -deoxycytidine hydrochloride, 0.005 part by weight of 2' -deoxyguanosine, 0.005 part by weight of thymidine, 0.005 part by weight of coenzyme A, 0.005 part by weight of coenzyme I, 0.005 part by weight of flavin adenine dinucleotide, 0.005 part by weight of pyridine nucleotide-sodium triphosphate, 0.005 part by weight of cocarboxylase, 0.005 part by weight of uracil nucleoside triphosphate, 5 parts by weight of sodium hyaluronate, 10 parts by weight of hydrolyzed sodium hyaluronate, 10 parts by weight of pentanediol, 30 parts by weight of butanediol, 20 parts by weight of hydroxyethyl urea and an appropriate amount of water are added into a container and mixed uniformly, heating to 80 deg.C, maintaining the temperature for 25min to dissolve completely to obtain mixture A;

Comparative example 10

To further illustrate the beneficial effects of the present invention, comparative example 10 is provided, which comparative example 10 differs from example 3 in that: the weight part of the lipoic acid in the whitening component is adjusted to 22 parts.

The comparative example whitening composition was prepared as follows:

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 22 parts of lipoic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methylpyrimidine carboxylic acid and 10 parts of allantoin, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 11

To further illustrate the beneficial effects of the present invention, comparative example 11 is provided, which comparative example 11 differs from example 3 in that: the weight portion of the sodium hyaluronate in the moisturizing component is adjusted to be 11 portions.

The comparative example whitening composition was prepared as follows:

s1, adding 0.5 part of potassium chloride, 0.5 part of sodium chloride, 0.5 part of calcium chloride, 0.5 part of sodium bicarbonate, 0.5 part of magnesium sulfate, 0.5 part of disodium hydrogen phosphate, 0.01 part of vitamin B2, 0.01 part of vitamin B6, 0.01 part of vitamin B7, 0.01 part of vitamin B9, 0.01 part of vitamin B12, 0.01 part of vitamin E, 0.01 part of lysine, 0.01 part of histidine, 0.01 part of arginine, 0.01 part of aspartic acid, 0.01 part of threonine, 0.01 part of serine, 0.01 part of glutamic acid, 0.01 part of proline, 0.01 part of glycine, 0.01 part of alanine, 0.01 part of valine, 0.01 part of leucine, 0.01 part of tyrosine, 0.01 part of phenylalanine, 0.005 part of coenzyme A, 0.005 part of 2 '-deoxyadenosine, 0.005 part of 2' -nucleotide, 0.005 part of thymidine, 0.10 part of adenine, 0.005, 10 part of thymidine, 0.10 part of sodium glutamate, 0.005, 10 parts of carboxyuridine, 10 parts of sodium glutamate, 10 parts of adenine, 10 parts of sodium glutamate, 10 parts of cysteine, 10 parts of disodium glutamate, 10 parts of cysteine, and water to a mixed in a container, heating to 80 deg.C, maintaining the temperature for 25min to dissolve completely to obtain mixture A;

Comparative example 12

To further illustrate the beneficial effects of the present invention, comparative example 12 is provided, which comparative example 12 differs from example 3 in that: the weight portion of the allantoin in the repair component is adjusted to 17 portions.

The comparative example whitening composition was prepared as follows:

s2, cooling the mixture A to 43 ℃ in a water bath, adding 15 parts of vitamin C, 10 parts of glutathione, 15 parts of nicotinamide, 10 parts of lipoic acid, 15 parts of tranexamic acid, 10 parts of arbutin, 20 parts of ceramide, 10 parts of tetrahydro-methylpyrimidine carboxylic acid and 17 parts of allantoin, uniformly stirring at 55rpm, and discharging at 35 ℃;

Comparative example 13

In order to further illustrate the beneficial effects of the invention, the invention provides a whitening essence containing siRNA cell culture for inhibiting melanin generation, which is prepared by adopting the method in CN 103860444A.

Experiment 1: skin Performance testing the Melanin (Melanin), erythema (erythma), and lightness (CIE-L) value data of the present invention were purchased from Beijing Dynasty & Toudo technologies, inc. in Denmark

Measured by a skin noninvasive detector. The transepidermal water loss (TEWL) data of the present invention was measured by Derma-ExpertMC 960, a skin diagnosis expert from CK, germany.

30 healthy volunteers of 25 to 30 years old were tested by recording Melanin value, erythema value, and CIE-L value of the test part of the volunteer before applying the whitening composition of the present invention, and then starting to continuously apply the whitening compositions prepared in examples 1 to 3 and comparative examples 1 to 13 to the face of the volunteer, respectively, at the cheek position of 10 × 10mm ² The skin with the area is 10 multiplied by 10mm selected from the symmetrical parts of the face of the same volunteer ² The Melanin value, the erythma value and the CIE-L value in the applied area are tested after 4 weeks by continuously applying the skin care product to the skin of the area once every morning and evening without applying any skin care product, and compared with the data before trial use, sixteen groups of data of trial examples 1-3 and comparative examples 1-13 are selected, and the data are shown in the following table 1. The Melanin value is a skin Melanin value which can indicate whether the skin is suntanned or not, and the Melanin value is not increased after the skin is used, so that the product has a sun-block black effect and the skin is not suntanned; the Erythema value is the Erythema value of the skin, the Erythema value is the sunburn value and indicates whether the skin is sunburned or not, and the Erythema value is not increased after the skin is used and indicates that the skin is not sunburned. The two data are combined to show the sun-screening effect of the whitening composition; the data in table 1 were measured for five sets of data for each test point and then averaged.

The TWEL value indicates the water loss rate of the epidermis of the skin within a period of time, indirectly indicates the barrier function of the skin, if the TEWL value is reduced, the water dispersion loss of the skin is reduced within a period of time, and the barrier function of the skin is improved, thereby indicating that the invention has the repairing function on the skin. The data in table 1 were measured for five sets of data for each test point and then averaged.

TABLE 1 skin care efficacy of whitening compositions using different formulation systems

From the above results, it can be seen that:

the melanin value is obviously reduced by using the whitening composition, which shows that the whitening composition has a sunscreen effect and can effectively prevent the skin from being tanned; after the whitening composition is used, the erythema value is obviously reduced, which shows that the whitening composition can effectively prevent the skin from being sunburned; after the whitening composition is used, the brightness value is obviously increased, which shows that the whitening composition has good whitening effect; meanwhile, after the whitening composition is used, the water loss rate is obviously reduced, the water dispersion loss of the skin is reduced, and the skin barrier function is improved, so that the whitening composition has a repairing effect on the skin, and the whitening composition achieves a more ideal skin care effect.

Whereas, after using the whitening composition other than the present invention (i.e., comparative example), the measured melanin value was significantly increased, i.e., the skin was tanned after using the whitening composition of the comparative example; after using the whitening composition of the comparative example, the erythema value was also increased even though the skin was sunburned after using the whitening composition of the comparative example; meanwhile, after the whitening composition of the comparative example is used, the water loss rate is basically unchanged, and the skin barrier function is not improved, namely, the whitening composition of the comparative example does not achieve a good skin care effect.

Experiment 2: stability test samples: the samples prepared in inventive examples 1-3 and comparative examples 1-13.

The experimental method comprises the following steps:

the tested samples are tested for 10 days, 30 days and 50 days under four test conditions of-18 ℃, room temperature, 45 ℃ and-18-45 ℃ alternately, and the tested samples are observed to have obvious change, pass or fail, and the test results are shown in the following table 2.

Table 2 stability test results

From the above results, it can be seen that:

after the whitening combination product is tested for 10 days, 30 days and 50 days under four test conditions of-18 ℃, room temperature, 45 ℃ and-18-45 ℃ alternately, the whitening combination product has no obvious change, and the test results are all qualified, namely the whitening combination product has good cold resistance and heat resistance and can meet the use requirements under the condition of extreme temperature change. The whitening composition without the formula system of the invention has poor cold resistance and heat resistance under long-term storage conditions, and cannot meet the use requirements under extreme temperature change conditions.

It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims

1. A whitening composition comprising a cell culture fluid component, a whitening component, a repairing component, and a moisturizing component, wherein the cell culture fluid comprises inorganic salts, vitamins, amino acids, ribonucleosides, and coenzymes;

the inorganic salt is selected from potassium chloride, sodium chloride, calcium chloride, sodium bicarbonate, magnesium sulfate and disodium hydrogen phosphate;

the vitamins are selected from vitamin B2, vitamin B6, vitamin B7, vitamin B9, vitamin B12 and vitamin E;

the amino acid is selected from lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine and phenylalanine;

said ribonucleoside is selected from the group consisting of 2' -deoxyadenosine, 2' -deoxycytidine hydrochloride, 2' -deoxyguanosine, and thymidine;

the coenzyme is selected from coenzyme A, coenzyme I, flavin adenine dinucleotide, pyridine nucleotide triphosphate-sodium, cocarboxylase and uracil nucleoside triphosphate;

the whitening composition further comprises a whitening ingredient selected from one or more of vitamin C, glutathione, niacinamide, lipoic acid, tranexamic acid and arbutin;

the whitening composition further comprises a repair ingredient selected from one or more of ceramide, tetrahydro-methyl pyrimidine carboxylic acid and allantoin;

the whitening composition further comprises a moisturizing ingredient selected from one or more of sodium hyaluronate, hydrolyzed sodium hyaluronate, pentanediol, butanediol, and hydroxyethyl urea;

the weight portion of the potassium chloride is 10 ^-3 To 1, the weight portion of the sodium chloride is 10 ^-3 To 1, the weight portion of the calcium chloride is 10 ^-3 To 1, the weight portion of the sodium bicarbonate is 10 ^-3 To 1, the weight part of the magnesium sulfate is 10 ^-3 To 1, the weight portion of the disodium hydrogen phosphate is 10 ^-3 To 1, the weight portion of the vitamin B2 is 10 ^-6 To 1, the weight portion of the vitamin B6 is 10 ^-6 To 1, the weight portion of the vitamin B7 is 10 ^-6 To 1, the weight portion of the vitamin B9 is 10 ^-6 To 1, the weight portion of the vitamin B12 is 10 ^-6 To 1, the weight portion of the vitamin E is 10 ^-6 To 1, the weight portion of the lysine is 10 ^-6 To 1, the weight portion of the histidine is 10 ^-6 To 1, the weight portion of the arginine is 10 ^-6 To 1, the weight portion of the aspartic acid is 10 ^-6 To 1, the weight portion of threonine is 10 ^-6 To 1, the weight portion of the serine is 10 ^-6 To 1, the weight portion of the glutamic acid is 10 ^-6 To 1, the proline accounts for 10 parts by weight ^-6 To 1, the weight portion of the glycine is 10 ^-6 To 1, the alanine accounts for 10 parts by weight ^-6 To 11, the valine is in a weight part of 10 ^-6 To 1, the isoleucine accounts for 10 parts by weight ^-6 To 1, the weight portion of the leucine is 10 ^-6 To 1, the weight portion of the tyrosine is 10 ^-6 To 1, the weight portion of the phenylalanine is 10 ^-6 To 1, the weight portion of the 2' -deoxyadenosine is 10 ^-8 To 1, said 2-The weight portion of the deoxycytidine hydrochloric acid is 10 ^-8 To 1, the weight portion of the 2' -deoxyguanosine is 10 ^-8 To 1, the thymidine is 10 parts by weight ^-8 To 1, the weight portion of the coenzyme A is 10 ^-8 To 1, the weight portion of the coenzyme I is 10 ^-8 To 1, the weight portion of the flavin adenine dinucleotide is 10 ^-8 To 1, the weight portion of the pyridine nucleotide triphosphate-sodium is 10 ^-8 To 1, the weight portion of the cocarboxylase is 10 ^-8 To 1, the weight portion of the uracil nucleoside triphosphate is 10 ^-8 1 to 1;

the vitamin C accounts for 0.01 to 20 parts by weight, the glutathione accounts for 0.01 to 15 parts by weight, the nicotinamide accounts for 0.1 to 20 parts by weight, the lipoic acid accounts for 0.01 to 20 parts by weight, the tranexamic acid accounts for 0.01 to 20 parts by weight, and the arbutin accounts for 0.01 to 15 parts by weight;

0.01 to 30 parts by weight of ceramide, 0.01 to 15 parts by weight of tetrahydromethylpyrimidine carboxylic acid and 0.01 to 15 parts by weight of allantoin;

the weight portion of the sodium hyaluronate is 0.001 to 10, the weight portion of the hydrolyzed sodium hyaluronate is 0.001 to 15, the weight portion of the pentanediol is 0.1 to 20, the weight portion of the butanediol is 0.1 to 50, and the weight portion of the hydroxyethyl urea is 0.1 to 80;

the preparation method of the whitening composition comprises the following steps:

s1, adding potassium chloride, sodium chloride, calcium chloride, sodium bicarbonate, magnesium sulfate, disodium hydrogen phosphate, vitamin B2, vitamin B6, vitamin B7, vitamin B9, vitamin B12, vitamin E, lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, 2' -deoxyadenosine, 2' -deoxycytidine hydrochloric acid, 2' -deoxyguanosine, thymidine, coenzyme A, coenzyme I, flavin adenine dinucleotide, pyridine nucleotide-sodium triphosphate, cocarboxylase, uracil nucleoside triphosphate, sodium hyaluronate, hydrolyzed sodium hyaluronate, pentanediol, butanediol, hydroxyethyl urea and a proper amount of water into a container, uniformly mixing, heating to 75 ℃, and keeping the temperature for 20min until the mixture is completely dissolved to obtain a mixture A;

s2, cooling the mixture A to 40 ℃ in a water bath, adding vitamin C, glutathione, nicotinamide, lipoic acid, tranexamic acid, arbutin, ceramide, tetrahydro-methyl pyrimidine carboxylic acid and allantoin, stirring uniformly at 50rpm, and discharging at 33 ℃;

2. A whitening product, wherein said whitening product comprises the whitening composition of claim 1.

3. Use of the whitening composition of claim 1 in the preparation of a whitening product.