CN110663385B - Novel application of novel compound in improving potassium ion stress resistance of Malus hupehensis Rehd - Google Patents
Novel application of novel compound in improving potassium ion stress resistance of Malus hupehensis Rehd Download PDFInfo
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- CN110663385B CN110663385B CN201911012784.9A CN201911012784A CN110663385B CN 110663385 B CN110663385 B CN 110663385B CN 201911012784 A CN201911012784 A CN 201911012784A CN 110663385 B CN110663385 B CN 110663385B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention relates to a novel application of a novel compound in improving potassium ion stress resistance of Malus hupehensis Rehd. The product taking strigolactone and resveratrol as effective components is applied to plants stressed by potassium ions, so that the capacity of the plants to remove active oxygen can be obviously enhanced, the oxidative damage of the plants caused by the potassium ions can be reduced, and the capacity of the plants to resist the potassium ions can be obviously improved.
Description
Technical Field
The invention relates to a novel application of a novel compound in improving potassium ion stress resistance of Malus hupehensis Rehd.
Background
Salt stress is one of the important environmental factors affecting plant growth, crop yield and quality, and soil salinization is also the most serious global problem facing the world today. At present, more than 20% of the land in the world is affected by salinization, and the trend is continuously expanding, and about 1 hundred million hectares of soil in China is affected by excessive salt. Potassium ion is a key factor involved in many physiological and biochemical reactions, plays an important role in maintaining cell activity, and excessive potassium ion causes plant damage, so that the potassium ion concentration in cells must be strictly maintained.
Apple is one of the most widely cultivated fruits in the world, but plant growth and fruit yield are severely affected due to soil salinity accumulation. Excessive potassium ions can disrupt the ionic balance and osmotic pressure in the body, producing large amounts of reactive oxygen species, resulting in oxidative damage. A large amount of soluble salt can reduce the soil water potential, change the cell osmotic potential and cause physiological drought. Therefore, the improvement of the potassium ion stress resistance of the cultivated plants is imminent, which is of great importance in agricultural production.
Excess reactive oxygen species produced under potassium stress are important factors in plant damage. Plants therefore have evolved an antioxidant system to alleviate salt stress damage. The antioxidant system includes superoxide dismutase, catalase and peroxidase, and when the plant is subjected to salt stress, various antioxidant enzymes such as superoxide dismutase, catalase and peroxidase in the antioxidant system act, and the plant removes excessive active oxygen in the body by using the antioxidant enzymatic removal system, and reduces oxidative damage of the plant by reducing the degree of plasma membrane peroxidation. However, in agricultural production, the antioxidant mechanism in plants is often insufficient to timely scavenge active oxygen caused by stress, thereby hindering plant growth.
The strigolactone is a sesquiterpene compound, the molecular skeleton of the strigolactone contains four rings, and the strigolactone is formed by connecting a tricyclic lactone with a gamma-butenolide ring through an enol ether bond, and the strigolactone is a novel plant hormone related to branching only discovered in recent years. Resveratrol is a polyphenol compound, also known as resveratrol, and is a natural antioxidant. However, no one researches the influence of the strigolactone and resveratrol compound on the apple potassium ion stress at present.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a novel application of a novel compound in improving the potassium ion stress resistance of the Malus hupehensis Rehd, which can obviously enhance the capacity of the plants for removing active oxygen, reduce the oxidative damage of the plants caused by the potassium ion stress and obviously improve the capacity of the plants for resisting salt stress.
In order to achieve the purpose, the invention adopts the following technical scheme:
a new application of a novel compound in improving the potassium ion stress resistance of plants is provided.
The effective components of the novel compound are strigolactone and resveratrol aqueous solution.
The concentration of the novel compound aqueous solution is 100 mu mol/L.
The novel compound aqueous solution is prepared by diluting 1mol/L of strigolactone and resveratrol ethanol solution.
The plant is Malus hupehensis Rehd.
The method for improving the potassium ion stress resistance of the plants comprises the following steps: the compound aqueous solution taking strigolactone and resveratrol as effective components is irrigated and sprayed on the leaf surfaces of the plant seedlings.
In the using step, the irrigation frequency is once in three days, and the total irrigation frequency is 5 times; the spraying frequency is once a day, and the total spraying frequency is 15 times.
The spraying amount of each plant is the same each time.
The invention has the beneficial effects that: the novel compound taking the strigolactone and the resveratrol as the active ingredients is applied to improving the potassium ion stress resistance of plants, so that the capacity of eliminating active oxygen of the plants can be obviously enhanced, the oxidative damage of the potassium ion stress to the plants is relieved, and the capacity of resisting the potassium ion stress of the plants is obviously improved, so that the plant wilting caused by the potassium ion is reduced, and the novel compound is convenient to apply and good in stability.
Detailed Description
The present invention will be further described with reference to the following examples.
Materials and reagents used in the following examples are all commercially available products unless otherwise specified.
The novel complex used in the following examples was a 100. mu. mol/L aqueous solution of strigolactone and resveratrol.
Example 1, a novel use of a novel complex comprising strigolactone and resveratrol as active ingredients for enhancing the resistance of Malus hupehensis Rehd to potassium stress.
Diluting 1mol/L strigolactone and resveratrol compound ethanol solution with distilled water to prepare 100 mu mol/L novel compound aqueous solution for later use.
Sowing the soaked 24h Malus hupehensis Rehd seeds into a pot filled with vermiculite, culturing for 20 days in a culture room at 25 ℃ and under the light intensity of 6lux, irrigating half culture solution for one week, and irrigating full nutrient solution for one week.
Firstly, selecting 32 Malus hupehensis seedling with consistent growth vigor and six leaves; then, dividing the selected Malus hupehensis Rehd seedlings into four groups, wherein each group comprises 8 seedlings; wherein, three groups are test groups, and one group is a control group.
Placing sterilized nutrient soil into small flowerpots (7 cm in length and width and 10cm in depth), transplanting selected Malus hupehensis seedling into the flowerpots respectively, irrigating a control group with distilled water containing 100mM potassium chloride, irrigating a test group with distilled water containing 100mM potassium chloride and 100. mu. mol/L of novel compound, wherein the volume of the irrigating solution is the same, and irrigating once every three days for a total period of 15 days.
And spraying prepared novel compound aqueous solution to the Malus hupehensis Rehd transplanted to the small flowerpot, wherein the compound aqueous solution with the concentration of 100 mu mol/L is sprayed to the three experimental groups respectively, the sprayed compound aqueous solution has the same volume, the solvent for preparing the compound aqueous solution with the same volume is sprayed to the plants of the control group once a day, and the total period is 15 days.
The activities of malondialdehyde, superoxide dismutase, catalase, peroxidase in each group of plants before and 15 days after treatment were measured and statistically analyzed, and the test was repeated three more times.
And (4) analyzing results: after the potassium chloride treatment is carried out for 15 days, the Malus hupehensis Rehd plant without the compound solution has wilting, the wilting rate is up to 66.67 percent, and obvious salt stress symptoms are shown; the potassium chloride stress resistance of the Malus hupehensis Rehd applied with the compound solution is enhanced, and the wilting rate is reduced to 33.33%. The experiments show that the growth condition of the Malus hupehensis Rehd under the stress of potassium chloride can be obviously improved by applying the novel compound aqueous solution externally.
The content of the plant endogenous malondialdehyde is an index for measuring the degree of oxidative damage of the plant, and the higher the content of the malondialdehyde is, the more serious the oxidative damage of the plant is. Through the determination of the content of endogenous malondialdehyde of a test plant, the content of endogenous malondialdehyde of the Malus hupehensis seedling is obviously increased from about 135 nmol/g.FW to about 219 nmol/g.FW after the potassium chloride stress treatment for 15 days. After the treatment with the aqueous complex solution, the malonaldehyde content is slightly increased compared with that before the treatment. The exogenous application of the novel compound aqueous solution can effectively reduce the content of endogenous malondialdehyde under the stress of potassium chloride.
After the Malus hupehensis seedling is treated by the compound aqueous solution for 15 days under the stress of potassium chloride, the activity of antioxidant enzymes such as superoxide dismutase, catalase and peroxidase in the Malus hupehensis seedling is obviously enhanced. Namely, under the stress of potassium chloride, the degree of oxidative damage of the plants sprayed with the compound aqueous solution is obviously lower than that of the plants not sprayed with the compound aqueous solution, and the experiments show that the exogenous spraying of the novel compound aqueous solution can effectively eliminate active oxygen caused by the stress of potassium chloride.
After the potassium chloride treatment for 15 days, the chlorophyll content in the Malus hupehensis Rehd leaves is remarkably reduced from about 41.3 mg/g-FW to about 36.25 mg/g-FW. After the treatment of the compound aqueous solution, the chlorophyll content in the Malus hupehensis Rehd leaf is slowly reduced under the stress of potassium chloride. After the potassium chloride stress treatment for 15 days, the photosynthesis rate of the Malus hupehensis Rehd leaf is obviously reduced from about 16.35 mu mol.m-2·s-1Down to about 3.15. mu. mol. m-2·s-1. After the treatment of the compound aqueous solution, the reduction of the photosynthesis rate of the Malus hupehensis Rehd under the stress of potassium chloride is obviously relieved. Therefore, the spraying of the novel compound aqueous solution can effectively remove active oxygen, slow down the degradation of chlorophyll and maintain the normal operation of photosynthesis under the stress of potassium chloride.
The experiments and data show that the potassium ion stress resistance of the Malus hupehensis Rehd can be obviously improved by spraying the novel compound aqueous solution.
Those not described in detail in this specification are within the knowledge of those skilled in the art.
The above-described embodiments are merely preferred embodiments of the present invention, and not intended to limit the scope of the invention, so that equivalent changes or modifications made based on the structures, features, and principles described in the claims of the present invention shall include the claims of the present invention.
Claims (8)
1. A new application of a novel compound containing strigolactone and resveratrol as effective components in improving potassium ion stress resistance of Malus hupehensis Rehd is provided.
2. The new application of claim 1, characterized in that: the novel compound is a compound aqueous solution with the effective components of strigolactone and resveratrol.
3. The new application of claim 2, characterized in that: the concentration of the novel compound aqueous solution is 100 mu mol/L.
4. The new application of claim 3, characterized in that: the novel compound aqueous solution is prepared by diluting 1mol/L of strigolactone and resveratrol ethanol solution.
5. The new application of claim 1, characterized in that: the Malus hupehensis Rehd is Malus hupehensis Rehd plant without apomixis.
6. The new application according to any one of claims 1 to 5, characterized by comprising the following steps: the novel compound with strigolactone and resveratrol as effective components is irrigated and sprayed on the leaf surfaces of the plant seedlings.
7. The new application of claim 6, wherein: in the step of irrigating and spraying, the irrigation frequency is once in three days, the total irrigation frequency is five times, the spraying frequency is once every day, and the total spraying frequency is 15 times.
8. The new application of claim 6, wherein: the amount of the water and the spraying amount of each plant are the same each time.
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