CN110643496B - Special culture inspection dish for bifidobacteria and use method - Google Patents
Special culture inspection dish for bifidobacteria and use method Download PDFInfo
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- 238000007689 inspection Methods 0.000 title claims abstract description 57
- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims description 12
- 238000007789 sealing Methods 0.000 claims abstract description 59
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 8
- 239000001301 oxygen Substances 0.000 claims abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000006916 nutrient agar Substances 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 4
- 238000010030 laminating Methods 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000009916 joint effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention belongs to the technical field of bacteria culture devices, and particularly relates to a special culture and inspection dish for bifidobacteria. Through the sealed recess of intermediate layer form and the mutual lock of the ware body that forms between two sealing strips, can reduce that the sealed department laminating of culture inspection ware is insufficient outside oxygen that leads to advances inside the culture inspection ware, cultivates the bifidobacterium of culture inspection ware and causes the influence, and it is narrower at one side width that is close to the ware lid roof to seal the recess of intermediate layer form, when carrying out with the mutual lock of the ware body, reaches and progressively pastes tight effect.
Description
Technical Field
The invention belongs to the technical field of bacteria culture devices, and particularly relates to a special culture inspection dish for bifidobacteria and a use method thereof.
Background
The bifidobacterium is a bacterial genus, is a gram-positive, immotile, rod-shaped, branched and strictly anaerobic bacterial genus with one end, is widely present in human and animal habitats such as digestive tract, vagina and oral cavity, is one of important components of human and animal intestinal flora, and can be used as probiotic bacteria in the aspects of food, medicine and feed.
The existing used bifidobacterium culture inspection dish has no pertinence in the use process, so that the bifidobacterium is insufficiently sealed in the culture inspection dish, the bifidobacterium needing to be cultured and inspected in an anaerobic environment cannot reach a strict anaerobic environment condition, the culture efficiency of the bifidobacterium is reduced, and meanwhile, the bifidobacterium in the culture dish can be leaked due to insufficient sealing in the process of culturing the bifidobacterium, so that the pollution is easily caused, and other bacteria are bred.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a special culture inspection dish for bifidobacteria and a using method thereof, which solve the problem that the existing bifidobacteria culture dish is poor in sealing under an anaerobic environment in the using process.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a be used for special cultivation inspection dish of bifidobacterium, includes the ware body, even be equipped with protection rubber section bottom the ware body, the surface of the ware body all is equipped with the sealed draw-in groove of annular, the top of the ware body is equipped with the ware lid, the lateral wall of ware lid is equipped with the sealing strip, be formed with the sealed recess of intermediate layer form between two sealing strips, the sealed recess of intermediate layer form can be buckled with ware body lateral wall mutually, the inside of the sealed recess of intermediate layer form is equipped with sealed fixture block, the ware body passes through the sealed draw-in groove of annular and sealed fixture block joint and links to each other with the ware lid, the toper through-hole has been seted up at the top of ware lid, one side of toper through-hole is equipped with annular spout, the inside of annular spout is equipped with the arc slider.
Preferably, the width of the sandwich-shaped sealing groove on one side close to the top plate of the dish cover is smaller than that on the other side.
Preferably, the annular sealing clamping groove is of a T-shaped structure.
Preferably, the number of the taper through holes is one, and the top of each taper through hole is provided with a rubber plug.
Preferably, the rubber stopper is not higher than the bottom of the movable shielding plate.
Preferably, the overall height of the special culture and inspection dish for the bifidobacteria is 5-6 cm.
In order to be matched with the culture dish for use, the use method of the special culture inspection dish for the bifidobacteria is provided, and comprises the following steps:
A. firstly, cleaning and drying a dish body (1) and a dish cover (4) of a culture inspection dish, and then carrying out ultraviolet sterilization on the dried culture dish for 15-30 min and storing for later use;
B. taking out the sterilized culture dish for later use, and buckling the dish cover (4) and the dish body (1) to realize the effect of sealing and isolating oxygen on the bifidobacterium culture inspection dish;
C. when the dish cover (4) is buckled, the dish cover (4) is rotated clockwise or anticlockwise by hands, clamping and sealing between the annular sealing clamping groove (3) and the sealing clamping block (6) are realized, and air is pumped out of the culture dish through the conical through hole (7) at the top of the dish cover (4) after sealing;
D. putting a nutrient agar culture medium into a culture dish, putting bifidobacteria into the culture dish through the tapered through hole (7), and finally sealing the tapered through hole (7) by using a rubber plug (8);
E. putting the whole culture inspection dish into a bacterial incubator for culture at the temperature of 30-40 ℃; the humidity is 20% -45%;
F. after culturing for 36-48 hours, the movable shielding plate (11) at the top of the dish cover (4) is rotated to realize direct inspection of the special culture dish for bifidobacterium, so that the pollution to the interior of the culture dish caused by the inspection of opening the dish cover (4) is avoided;
G. and after the bifidobacterium culture dish is inspected, opening the rubber plug (8) at the top of the rotating dish cover (4), and then sequentially dismantling the culture inspection dish and carrying out cleaning and disinfection treatment for the next use.
Compared with the prior art, the invention has the beneficial effects that:
this be used for special cultivation inspection dish of bifidobacterium, sealed recess of intermediate layer form and the mutual lock of the ware body through forming between two sealing strips, can reduce the cultivation inspection dish and seal that the laminating of department is not abundant outside oxygen that leads to and advance inside the cultivation inspection dish, the bifidobacterium to cultivateing the inspection dish cultivates and causes the influence, it is narrower at one side width that is close to the ware lid roof at the sealed recess of intermediate layer form, when carrying out with the mutual lock of the ware body, reach and progressively paste tight effect, can utilize joint between annular seal draw-in groove and the sealed fixture block to further seal the reinforcement to the cultivation inspection dish simultaneously, take place to become flexible with the reduction cultivation inspection dish interface, the oxygen that causes gets into the cultivation inspection dish.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a first perspective view of the present invention;
FIG. 2 is a second perspective view of the present invention;
FIG. 3 is a front view of the present invention;
fig. 4 is a cross-sectional view of a three-dimensional structure of the present invention.
In the figure: 1, a dish body; 2 a protective rubber section; 3, an annular sealing clamping groove; 4, closing the dish; 5, sealing strips; 6, sealing the fixture block; 7, a conical through hole; 8, rubber plugs; 9, an annular chute; 10, an arc-shaped sliding block; 11 a movable shutter.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides the following technical solutions: a special culture and inspection dish for bifidobacteria comprises a dish body 1, wherein the bottom of the dish body 1 is uniformly provided with a protective rubber section 2, the surface of the dish body 1 is provided with an annular sealing clamping groove 3, the top of the dish body 1 is provided with a dish cover 4, the side wall of the dish cover 4 is provided with a sealing strip 5, a sandwich-shaped sealing groove is formed between the two sealing strips 5 and can be buckled with the side wall of the dish body 1, the sandwich-shaped sealing groove formed between the two sealing strips 5 is buckled with the dish body 1, so that the external oxygen entering the culture and inspection dish due to insufficient joint at the sealing part of the culture and inspection dish can be reduced to influence the bifidobacteria culture of the culture and inspection dish, the width of one side of the sandwich-shaped sealing groove close to the top plate of the dish cover 4 is narrow, when the sandwich-shaped sealing groove is buckled with the dish body 1, the gradual joint effect is achieved, the sealing clamping block 6 is arranged inside the sandwich-, the ware body 1 links to each other with ware lid 4 through annular seal draw-in groove 3 and 6 joints of sealed fixture block, can utilize the joint between annular seal draw-in groove 3 and the sealed fixture block 6 further to seal up the cultivation inspection ware simultaneously, take place to become flexible in order to reduce the cultivation inspection ware interface, the oxygen that causes gets into the cultivation inspection ware, toper through-hole 7 has been seted up at the top of ware lid 4, one side of toper through-hole 7 is equipped with annular spout 9, the inside of annular spout 9 is equipped with arc slider 10, ware lid 4 has activity sunshade 11 through arc slider 10 swing joint.
Specifically, the width of the interlayer-shaped sealing groove on one side close to the top plate of the dish cover 4 is smaller than that on the other side, the width of one side close to the top plate of the dish cover 4 through the arranged interlayer-shaped sealing groove is narrower, and the effect of gradually attaching to the dish body 1 can be achieved when the dish body is buckled.
Specifically, the annular sealing clamping groove 3 is of a T-shaped structure, the annular sealing clamping groove 3 is of a symmetrical structure, clockwise or anticlockwise rotation clamping in two directions can be achieved when the clamping connection is carried out with the sealing clamping block 6, and the clamping device is simple in structure and convenient to use.
Specifically, the quantity of toper through-hole 7 is one, and 7 tops of toper through-hole are equipped with rubber buffer 8, through the rubber buffer 8 that sets up, can be under the condition of not opening culture dish ware lid 4, add the material or carry out gas exchange to culture dish inside, and the opening of toper through-hole 7 is less simultaneously, can reduce the pollution that causes culture dish inside when opening.
Specifically, the height of the rubber plug 8 is not higher than the bottom of the movable shielding plate 11, and the rubber plug 8 does not obstruct the rotation of the movable shielding plate 11 when the movable shielding plate 11 is rotated by arranging the movable shielding plate 11 higher than the rubber plug 8.
Specifically, this a whole height that is used for special culture inspection dish of bifidobacterium is 5~6cm, and the whole height through the culture inspection dish that sets up is 5~6cm, and the volume is less, and simple structure can be convenient for store and observe the use.
In order to be matched with the culture dish for use, the use method of the special culture inspection dish for the bifidobacteria is provided, and comprises the following steps:
A. firstly, cleaning and drying a dish body (1) and a dish cover (4) of a culture inspection dish, and then carrying out ultraviolet sterilization on the dried culture dish for 15-30 min and storing for later use;
B. taking out the sterilized culture dish for later use, and buckling the dish cover (4) and the dish body (1) to realize the effect of sealing and isolating oxygen on the bifidobacterium culture inspection dish;
C. when the dish cover (4) is buckled, the dish cover (4) is rotated clockwise or anticlockwise by hands, clamping and sealing between the annular sealing clamping groove (3) and the sealing clamping block (6) are realized, and air is pumped out of the culture dish through the conical through hole (7) at the top of the dish cover (4) after sealing;
D. putting a nutrient agar culture medium into a culture dish, putting bifidobacteria into the culture dish through the tapered through hole (7), and finally sealing the tapered through hole (7) by using a rubber plug (8);
E. putting the whole culture inspection dish into a bacterial incubator for culture at the temperature of 30-40 ℃; the humidity is 20% -45%;
F. after culturing for 36-48 hours, the movable shielding plate (11) at the top of the dish cover (4) is rotated to realize direct inspection of the special culture dish for bifidobacterium, so that the pollution to the interior of the culture dish caused by the inspection of opening the dish cover (4) is avoided;
G. and after the bifidobacterium culture dish is inspected, opening the rubber plug (8) at the top of the rotating dish cover (4), and then sequentially dismantling the culture inspection dish and carrying out cleaning and disinfection treatment for the next use.
The working principle and the using process of the invention are as follows: after the culture dish is installed, the dish cover 4 is buckled, the interlayer-shaped sealing groove formed in the side wall of the dish cover 4 is buckled with the side wall of the dish body 1, the annular sealing clamping groove 3 in the surface of the dish body 1 is clamped with the sealing clamping block 6 at the bottom of the dish cover 4 by rotating the dish cover 4, the oxygen-isolating sealing effect of the culture dish is achieved, meanwhile, air in the culture dish is exhausted through the tapered through hole 7 in the top of the dish cover 4, a culture medium is firstly added into the culture dish with the air being removed, bifidobacteria are then placed into the culture dish body 1 through the tapered through hole 7, after a proper amount of bifidobacteria is added, the air is exhausted through the tapered through hole 7, after the air is exhausted, the culture dish is sealed by covering the rubber plug 8, and the culture dish can be observed by rotating the movable shielding plate 11.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The utility model provides a be used for special culture inspection dish of bifidobacterium, includes dish body (1), its characterized in that: the bottom of the dish body (1) is uniformly provided with a protective rubber section (2), the surface of the dish body (1) is provided with an annular sealing clamping groove (3), the top of the dish body (1) is provided with a dish cover (4), the side wall of the dish cover (4) is provided with sealing strips (5), a sandwich-shaped sealing groove is formed between the two sealing strips (5), the interlayer-shaped sealing groove can be buckled with the side wall of the dish body (1), a sealing fixture block (6) is arranged inside the interlayer-shaped sealing groove, the dish body (1) is connected with the dish cover (4) through the clamping of an annular sealing clamping groove (3) and a sealing clamping block (6), a conical through hole (7) is arranged at the top of the dish cover (4), an annular sliding groove (9) is arranged at one side of the conical through hole (7), an arc-shaped sliding block (10) is arranged inside the annular sliding groove (9), and the dish cover (4) is movably connected with a movable shielding plate (11) through the arc-shaped sliding block (10).
2. The special culture inspection dish for bifidobacteria according to claim 1, wherein: the width of the interlayer-shaped sealing groove at one side close to the top plate of the dish cover (4) is smaller than that at the other side.
3. The special culture inspection dish for bifidobacteria according to claim 1, wherein: the annular sealing clamping groove (3) is of a T-shaped structure.
4. The special culture inspection dish for bifidobacteria according to claim 1, wherein: the number of the conical through holes (7) is one, and rubber stoppers (8) are arranged at the tops of the conical through holes (7).
5. The special culture inspection dish for bifidobacteria according to claim 1, wherein: the height of the rubber plug (8) is not higher than the bottom of the movable shielding plate (11).
6. The special culture inspection dish for bifidobacteria according to claim 1, wherein: the overall height of the special culture inspection dish for the bifidobacteria is 5-6 cm.
7. The use method of the special culture inspection dish for bifidobacteria according to claim 1, wherein the special culture inspection dish comprises: the method specifically comprises the following steps:
A. firstly, cleaning and drying a dish body (1) and a dish cover (4) of a culture inspection dish, and then carrying out ultraviolet sterilization on the dried culture dish for 15-30 min and storing for later use;
B. taking out the sterilized culture dish for later use, and buckling the dish cover (4) and the dish body (1) to realize the effect of sealing and isolating oxygen on the bifidobacterium culture inspection dish;
C. when the dish cover (4) is buckled, the dish cover (4) is rotated clockwise or anticlockwise by hands, clamping and sealing between the annular sealing clamping groove (3) and the sealing clamping block (6) are realized, and air is pumped out of the culture dish through the conical through hole (7) at the top of the dish cover (4) after sealing;
D. putting a nutrient agar culture medium into a culture dish, putting bifidobacteria into the culture dish through the tapered through hole (7), and finally sealing the tapered through hole (7) by using a rubber plug (8);
E. putting the whole culture inspection dish into a bacterial incubator for culture at the temperature of 30-40 ℃; the humidity is 20% -45%;
F. after culturing for 36-48 hours, the movable shielding plate (11) at the top of the dish cover (4) is rotated to realize direct inspection of the special culture dish for bifidobacterium, so that the pollution to the interior of the culture dish caused by the inspection of opening the dish cover (4) is avoided;
G. and after the bifidobacterium culture dish is inspected, opening the rubber plug (8) at the top of the rotating dish cover (4), and then sequentially dismantling the culture inspection dish and carrying out cleaning and disinfection treatment for the next use.
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| US4033826A (en) * | 1975-11-20 | 1977-07-05 | Larsen Otis M | Anaerobic system container |
| CN202246681U (en) * | 2011-09-22 | 2012-05-30 | 林毅 | Microbial culture dish |
| CN206736252U (en) * | 2017-05-11 | 2017-12-12 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactic acid bacteria Rapid Fermentation culture dish |
| CN109825425A (en) * | 2019-02-19 | 2019-05-31 | 江苏中新医药有限公司 | A kind of air-tightness culture dish of generation and maintenance anaerobic or micro- oxygen microenvironment |
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- 2019-10-07 CN CN201910947029.3A patent/CN110643496B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4033826A (en) * | 1975-11-20 | 1977-07-05 | Larsen Otis M | Anaerobic system container |
| CN202246681U (en) * | 2011-09-22 | 2012-05-30 | 林毅 | Microbial culture dish |
| CN206736252U (en) * | 2017-05-11 | 2017-12-12 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactic acid bacteria Rapid Fermentation culture dish |
| CN109825425A (en) * | 2019-02-19 | 2019-05-31 | 江苏中新医药有限公司 | A kind of air-tightness culture dish of generation and maintenance anaerobic or micro- oxygen microenvironment |
Non-Patent Citations (1)
| Title |
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| 实验室简易微生物厌氧培养装置;范志恒;《江苏食品与发酵》;19971231(第03期);第5-6页 * |
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Effective date of registration: 20210929 Address after: 238200 Hefei vocational and technical college, Chaohu Economic Development Zone, Hefei City, Anhui Province Patentee after: HEFEI TECHNOLOGY College Address before: 238000 Hefei vocational and technical college, Laoshan South Road, Chaohu Economic Development Zone, Hefei City, Anhui Province Patentee before: Xu Zhenguo |
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