CN110628619A - Cell engineering multidimension degree cell oscillation device - Google Patents
Cell engineering multidimension degree cell oscillation device Download PDFInfo
- Publication number
- CN110628619A CN110628619A CN201910996924.4A CN201910996924A CN110628619A CN 110628619 A CN110628619 A CN 110628619A CN 201910996924 A CN201910996924 A CN 201910996924A CN 110628619 A CN110628619 A CN 110628619A
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- plate
- cell
- fixedly connected
- assembly
- supporting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
Abstract
The invention discloses a cell engineering multidimensional cell oscillation device, which belongs to the technical field of cell engineering and comprises a base, a moving plate, a horizontal driving assembly, a supporting assembly, a rotary vibration assembly, a shaking assembly, a cell placing assembly and two guide rails, wherein the two guide rails are symmetrically arranged on the base, the moving plate is arranged on the two guide rails and is in sliding fit with the two guide rails, the horizontal driving assembly is arranged beside the two guide rails, one end of the horizontal driving assembly is fixedly connected with the moving plate, the supporting assembly is arranged on the moving plate, the rotary vibration assembly is arranged at the top end of the supporting assembly, the shaking assembly is arranged at the top end of the rotary vibration assembly, and the cell placing assembly is arranged on the shaking assembly. The invention solves the problems that the cell solution can not be vibrated in multiple directions and the vibrating effect is not ideal.
Description
Technical Field
The invention relates to the technical field of cell engineering, in particular to a cell engineering multidimensional cell oscillation device.
Background
Shake culture, also known as shake flask culture. It is a culture method in which microbial cells are inoculated in a liquid culture medium and placed on a shaking table or a shaker to shake continuously. The shake can make the culture medium fully contact with oxygen, improve the supply of dissolved oxygen, the thallus after shake culture breeds the homogeneous, the culture efficiency is high, apply to strain screening and microorganism expanded culture extensively, it is the common culture mode in microorganism physiology, biochemistry, fermentation and other life science research fields. The existing cell oscillation device cannot oscillate cell solution in multiple directions, and the oscillation effect is not ideal.
Disclosure of Invention
The invention aims to solve the technical problem of providing a cell engineering multidimensional cell oscillation device to solve the technical problems that the cell solution cannot be oscillated in multiple directions and the oscillation effect is not ideal in the prior art.
In order to solve the technical problems, the invention provides the following technical scheme: the utility model provides a cell engineering multidimension degree cell oscillation device, includes base, movable plate, horizontal drive subassembly, supporting component, rotatory vibration subassembly, rocks subassembly, cell and places subassembly and two guide rails, two the guide rail is the symmetry and sets up on the base, the movable plate is installed two on the guide rail, and movable plate and two guide rail sliding fit, horizontal drive subassembly sets up two the side of guide rail, and horizontal drive subassembly's one end with movable plate fixed connection, the supporting component is installed on the movable plate, rotatory vibration subassembly sets up supporting component's top, it sets up to rock the subassembly rotatory vibration subassembly's top, the cell is placed the unit mount and is in rock on the subassembly.
Further, the horizontal driving assembly comprises a vertical plate, a first sliding column, a second sliding column, an elliptical plate, a push plate, a first driving motor, a semicircular gear and two sliding seats, the vertical plate is vertically arranged on the base, the two sliding seats are symmetrically arranged on the side wall of the vertical plate, the first sliding column and the second sliding column can be respectively slidably arranged on the two sliding seats, two ends of the elliptical plate are respectively fixedly connected with the rear end of the first sliding column and the front end of the second sliding column, the elliptical plate is provided with a first rack vertically, two ends of the push plate are respectively fixedly connected with the side wall of the movable plate and the front end of the first sliding column, the first driving motor is horizontally arranged on the side wall of the vertical plate, and an output shaft of the first driving motor extends into the elliptical plate, the semicircular gear is fixedly connected with an output shaft of the first driving motor, and the semicircular gear is meshed with the first rack.
Further, the supporting component includes backup pad and four spring support piece, the backup pad sets up the top of movable plate, four spring support piece is the rectangle and distributes on the movable plate, and four spring support piece's top with movable plate fixed connection, every spring support piece all includes fixing base, lower fixing base, spacing post and supporting spring, go up the fixing base with the bottom fixed connection of backup pad, down the fixing base with the top fixed connection of movable plate, spacing post sets up in the fixing base down, the supporting spring cover is established on the spacing post, and supporting spring's both ends respectively with go up the fixing base with fixing base fixed connection down.
Further, rotatory vibration subassembly includes circular platform, axis of rotation, rolling disc, second driving motor, driving gear, driven gear and two vibrating motor, the circular platform is vertical setting and is in the backup pad, the axis of rotation can rotate and install on the circular platform, and the bottom of axis of rotation extends to in the circular platform, the rolling disc with the top fixed connection of axis of rotation, the second driving motor is vertical setting and is in the circular platform, the driving gear with second driving motor's output shaft fixed connection, driven gear with the bottom fixed connection of axis of rotation, and driven gear with driving gear meshing, two vibrating motor is the symmetry and sets up on the lateral wall of circular platform.
Furthermore, the shaking assembly comprises a transverse plate and two shaking pieces, the two shaking pieces are symmetrically arranged on the rotating disc, two ends of the transverse plate are respectively and fixedly connected with one ends of the two shaking pieces, each shaking piece comprises a bearing plate, a sliding plate, a second rack, a mounting frame, a third driving motor and a rotating gear, the bearing plate is vertically arranged on the rotating disc, an opening is arranged at the top end of the bearing plate, the sliding plate can be slidably arranged in the bearing plate, the side wall of the sliding plate is fixedly connected with one end of the transverse plate, the second rack is fixedly connected with the side wall of the sliding plate, the mounting frame is arranged beside the bearing plate, the third driving motor is horizontally arranged at the top end of the mounting frame, and the rotating gear is fixedly connected with an output shaft of the third driving motor, and the rotation gear is engaged with the second rack.
Further, the cell placing assembly comprises an upper pressing plate, a lower pressing plate, a cell placing bottle, two fastening bolts and four connecting springs, the lower pressing plate is fixedly connected with the transverse plate, the upper pressing plate is installed at the top of the lower pressing plate through the four connecting springs, the cell placing bottle is arranged between the upper pressing plate and the lower pressing plate, the two fastening bolts are installed on the upper pressing plate and the lower pressing plate, and a bottle cap in threaded connection with the inner wall of the cell placing bottle is arranged at the front end of the cell placing bottle.
Furthermore, the bottom of the base is provided with four support columns which are distributed in a rectangular shape.
Compared with the prior art, the invention has the beneficial effects that:
firstly, cell sap is placed in the cell placing assembly manually, then the horizontal driving assembly works to drive the movable plate to reciprocate back and forth on the two guide rails, the movable plate drives the cell placing assembly to reciprocate back and forth synchronously, the cell sap in the cell placing assembly reciprocates back and forth to generate vibration, meanwhile, the rotary vibration assembly works to drive the cell placing assembly to rotate and vibrate, the cell sap in the cell placing assembly rotates and vibrates along with the cell depositing assembly, the vibration is more sufficient, then the cell placing assembly works to drive the cell placing assembly to ascend and descend and vibrate back and forth, the cell sap in the cell placing assembly shakes along with the cell depositing assembly, the vibration degree is intensified, the purpose of shaking the cell sap in multiple directions is achieved, and the vibration effect is better.
Secondly, the cell placement assembly drives the semicircular gear fixedly connected with the output shaft of the cell placement assembly to rotate through the work of the first driving motor, and the semicircular gear is meshed with the first rack, so that the semicircular gear drives the elliptical plate to reciprocate back and forth, the elliptical plate drives the first sliding column and the second sliding column to reciprocate back and forth on the two sliding seats, the first sliding column drives the push plate to synchronously reciprocate back and forth, the push plate drives the moving plate to reciprocate back and forth on the two guide rails, and the cell liquid in the cell placement assembly reciprocates back and forth to generate oscillation.
Thirdly, the rotating vibration component is supported and damped through the supporting spring, the movable plate is prevented from being driven to vibrate along with the rotating vibration component when the rotating vibration component vibrates, and the movable plate is prevented from being influenced to reciprocate on the two guide rails.
Fourthly, the driving gear fixedly connected with an output shaft of the second driving motor is driven to rotate through the work of the second driving motor, the driving gear is meshed with the driven gear, the driving gear drives the driven gear to synchronously rotate, the driven gear drives the rotating shaft to synchronously rotate, the rotating shaft drives the rotating disc to rotate along with the rotating shaft, the rotating disc drives the cell placing assembly to rotate along with the rotating shaft, the two vibrating motors also work to drive the circular table to vibrate while the second driving motor works, the circular table drives the rotating disc to vibrate along with the rotating shaft, the rotating disc drives the cell placing assembly to vibrate along with the rotating disc, the cell placing assembly rotates and vibrates, cell liquid in the cell placing assembly rotates and vibrates along with the rotating disc, and the vibration is more sufficient.
Fifth, the rotating gear fixedly connected with the output shaft of the third driving motor is driven to rotate through positive rotation of the third driving motor, and the rotating gear is meshed with the second rack, so that the rotating gear drives the sliding plate to move upwards in the bearing plate, the bearing plate drives the transverse plate to move upwards synchronously, the transverse plate drives the cell placement assembly to move upwards synchronously, and after the cell placement assembly moves upwards to a certain distance, the third driving motor works in a reverse rotation mode to drive the cell placement assembly to move downwards, so that the cell placement assembly repeatedly goes up and down to and fro and shakes, cell liquid in the cell placement assembly shakes along with the cell liquid, and the degree of shaking is intensified.
According to the invention, cell sap is poured into the cell placing bottle manually, then the bottle cap is screwed with the front end of the cell placing bottle, then the cell placing bottle is placed between the upper pressing plate and the lower pressing plate manually, and finally the cell placing bottle is tightly fixed between the upper pressing plate and the lower pressing plate by rotating the two fastening bolts, so that the cell placing bottle is prevented from falling off during vibration.
Drawings
FIG. 1 is a first perspective view of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic perspective view of the horizontal driving assembly of the present invention;
FIG. 4 is a schematic perspective view of a rotational vibration assembly according to the present invention;
FIG. 5 is a partial cross-sectional view of a rotary vibration assembly of the present invention;
FIG. 6 is a schematic perspective view of the shaking assembly and the cell placement assembly according to the present invention.
The reference numbers in the figures are: 1-a base; 11-a support column; 2, moving a board; 3-a horizontal drive assembly; 31-a riser; 32-a first sliding column; 33-a second sliding column; 34-an elliptical plate; 35-a push plate; 36-a first drive motor; 37-semicircular gear; 38-a slide mount; 39-a first rack; 4-a support assembly; 41-a support plate; 42-a spring support; 421-upper fixed seat; 422-lower fixed seat; 423-limit column; 424-support springs; 5-rotating the vibrating component; 51-circular table; 52-rotating shaft; 53-rotating disc; 54-a second drive motor; 55-a drive gear; 56-driven gear; 57-a vibration motor; 6-shaking the assembly; 61-a transverse plate; 62-shaking pieces; 621-a carrier plate; 622-sliding plate; 623-a second rack; 624-a mounting frame; 625-a third drive motor; 626-a rotating gear; 7-a cell placement component; 71-an upper platen; 72-a lower platen; 73-cell placement vial; 74-fastening bolts; 75-connecting a spring; 8-guide rail.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1 to 6, the invention provides a cell engineering multidimensional cell oscillation device, which includes a base 1, a moving plate 2, a horizontal driving assembly 3, a supporting assembly 4, a rotary oscillation assembly 5, a shaking assembly 6, a cell placement assembly 7 and two guide rails 8, wherein the two guide rails 8 are symmetrically arranged on the base 1, the moving plate 2 is mounted on the two guide rails 8, the moving plate 2 is in sliding fit with the two guide rails 8, the horizontal driving assembly 3 is arranged beside the two guide rails 8, one end of the horizontal driving assembly 3 is fixedly connected with the moving plate 2, the supporting assembly 4 is mounted on the moving plate 2, the rotary oscillation assembly 5 is arranged at the top end of the supporting assembly 4, and the shaking assembly 6 is arranged at the top end of the rotary oscillation assembly 5, cell placement component 7 is installed rock on the subassembly 6, place cell liquid in cell placement component 7 through the manual work, then 3 work of horizontal drive subassembly drive movable plate 2 reciprocating motion back and forth on two guide rails 8, movable plate 2 drives cell placement component 7 synchronous reciprocating motion back and forth, cell liquid in the subassembly 7 is placed to the cell is made a round trip reciprocating motion and is vibrated thereupon, 5 work of rotatory vibration subassembly drives cell placement component 7 simultaneously and rotates and vibrate, cell liquid in the subassembly 7 is placed to the cell rotates and vibrates thereupon, vibrate more fully, it shakes cell placement component 7 to make a round trip to go up and down to rock subassembly 6 work drive cell placement component 7 simultaneously again, cell liquid in the subassembly 7 is placed to the cell is rocked thereupon, the degree of aggravation vibration, reached the purpose that multiple direction vibrates the cell liquid, the effect of vibration is better.
The horizontal driving component 3 comprises a vertical plate 31, a first sliding column 32, a second sliding column 33, an elliptical plate 34, a push plate 35, a first driving motor 36, a semicircular gear 37 and two sliding seats 38, wherein the vertical plate 31 is vertically arranged on the base 1, the two sliding seats 38 are symmetrically arranged on the side wall of the vertical plate 31, the first sliding column 32 and the second sliding column 33 can be respectively and slidably arranged on the two sliding seats 38, two ends of the elliptical plate 34 are respectively fixedly connected with the rear end of the first sliding column 32 and the front end of the second sliding column 33, the elliptical plate 34 is provided with a first rack 39 from top to bottom, two ends of the push plate 35 are respectively fixedly connected with the side wall of the moving plate 2 and the front end of the first sliding column 32, the first driving motor 36 is horizontally arranged on the side wall of the vertical plate 31, and the output shaft of the first driving motor 36 extends into the elliptical plate 34, the semicircular gear 37 is fixedly connected with the output shaft of the first driving motor 36, the semicircular gear 37 is meshed with the first rack 39, the semicircular gear 37 fixedly connected with the output shaft of the first driving motor 36 is driven to rotate by the operation of the first driving motor 36, because the semicircular gear 37 is meshed with the first rack 39, the semicircular gear 37 drives the elliptical plate 34 to reciprocate back and forth, the elliptical plate 34 drives the first sliding column 32 and the second sliding column 33 to reciprocate back and forth on the two sliding seats 38, the first sliding column 32 drives the push plate 35 to synchronously reciprocate back and forth, the push plate 35 drives the moving plate 2 to reciprocate back and forth on the two guide rails 8, and the cell sap in the cell placement component 7 is vibrated along with the reciprocating back and forth.
The supporting component 4 comprises a supporting plate 41 and four spring supporting members 42, the supporting plate 41 is disposed above the moving plate 2, the four spring supporting members 42 are distributed on the moving plate 2 in a rectangular shape, the top ends of the four spring supporting members 42 are fixedly connected with the moving plate 2, each spring supporting member 42 comprises an upper fixing seat 421, a lower fixing seat 422, a limiting column 423 and a supporting spring 424, the upper fixing seat 421 is fixedly connected with the bottom of the supporting plate 41, the lower fixing seat 422 is fixedly connected with the top of the moving plate 2, the limiting column 423 is disposed in the lower fixing seat 422, the supporting spring 424 is sleeved on the limiting column 423, and two ends of the supporting spring 424 are respectively fixedly connected with the upper fixing seat 421 and the lower fixing seat 422, when the rotary vibration component 5 works, the rotary vibration component 5 is supported and damped by the supporting spring 424, the rotary vibration component 5 is prevented from driving the moving plate 2 to vibrate along with the rotary vibration component, and the moving plate 2 is prevented from being influenced to reciprocate on the two guide rails 8.
The rotary vibration assembly 5 comprises a circular table 51, a rotating shaft 52, a rotating disc 53, a second driving motor 54, a driving gear 55, a driven gear 56 and two vibration motors 57, wherein the circular table 51 is vertically arranged on the supporting plate 41, the rotating shaft 52 can be rotatably arranged on the circular table 51, the bottom end of the rotating shaft 52 extends into the circular table 51, the rotating disc 53 is fixedly connected with the top end of the rotating shaft 52, the second driving motor 54 is vertically arranged in the circular table 51, the driving gear 55 is fixedly connected with the output shaft of the second driving motor 54, the driven gear 56 is fixedly connected with the bottom end of the rotating shaft 52, the driven gear 56 is meshed with the driving gear 55, the two vibration motors 57 are symmetrically arranged on the outer side wall of the circular table 51, and the driving gear 55 fixedly connected with the output shaft thereof is driven to rotate by the second driving motor 54, because the driving gear 55 is meshed with the driven gear 56, the driving gear 55 drives the driven gear 56 to synchronously rotate, the driven gear 56 drives the rotating shaft 52 to synchronously rotate, the rotating shaft 52 drives the rotating disc 53 to rotate along with the rotating disc 53, the rotating disc 53 drives the cell placing assembly 7 to rotate along with the rotating disc 53, the two vibrating motors 57 also work to drive the circular table 51 to vibrate while the second driving motor 54 works, the circular table 51 drives the rotating disc 53 to vibrate along with the rotating shaft 52, the rotating disc 53 drives the cell placing assembly 7 to vibrate along with the rotating disc 53, the cell placing assembly 7 rotates and vibrates, the cell liquid in the cell placing assembly 7 rotates and vibrates along with the rotating disc, and the vibration is more sufficient.
The swinging assembly 6 comprises a transverse plate 61 and two swinging members 62, the two swinging members 62 are symmetrically arranged on the rotating disc 53, two ends of the transverse plate 61 are respectively and fixedly connected with one end of the two swinging members 62, each swinging member 62 comprises a bearing plate 621, a sliding plate 622, a second rack 623, a mounting frame 624, a third driving motor 625 and a rotating gear 626, the bearing plate 621 is vertically arranged on the rotating disc 53, an opening is arranged at the top end of the bearing plate 621, the sliding plate 622 can be slidably mounted in the bearing plate 621, the side wall of the sliding plate 622 and one end of the transverse plate 61 are fixedly connected, the second rack 623 and the side wall of the sliding plate 622 are fixedly connected, the mounting frame 624 is arranged at the side of the bearing plate 621, and the third driving motor 625 is horizontally arranged at the top end of the mounting frame 624, the rotating gear 626 is fixedly connected with the output shaft of the third driving motor 625, and the rotating gear 626 is engaged with the second rack 623, the rotating gear 626 fixedly connected with the output shaft is driven to rotate by the positive rotation of the third driving motor 625, because the rotating gear 626 is engaged with the second rack 623, the rotating gear 626 drives the sliding plate 622 to move upwards in the bearing plate 621, the bearing plate 621 drives the horizontal plate 61 to move upwards synchronously, the horizontal plate 61 drives the cell placing component 7 to move upwards synchronously, when the cell placing component 7 moves upwards to a certain distance, the third driving motor 625 rotates reversely to drive the cell placing component 7 to move downwards, so that the cell placing component 7 repeatedly goes up and down and swings back and forth, the cell liquid in the cell placing component 7 swings along with the cell liquid, and the vibration is intensified.
The cell placement assembly 7 comprises an upper pressing plate 71, a lower pressing plate 72, a cell placement bottle 73, two fastening bolts 74 and four connecting springs 75, wherein the lower pressing plate 72 is fixedly connected with the transverse plate 61, the upper pressing plate 71 is arranged on the top of the lower pressing plate 72 through the four connecting springs 75, the cell placement bottle 73 is arranged between the upper pressing plate 71 and the lower pressing plate 72, the two fastening bolts 74 are arranged on the upper pressing plate 71 and the lower pressing plate 72, the front end of the cell placement bottle 73 is provided with a bottle cap screwed with the inner wall thereof, cell sap is poured into the cell placement bottle 73 by manpower, then the bottle cap is screwed with the front end of the cell placement bottle 73, then the cell placement bottle 73 is manually placed between the upper pressing plate 71 and the lower pressing plate 72, and finally the two fastening bolts 74 are rotated to tightly fix the cell placement bottle 73 between the upper pressing plate 71 and the lower pressing plate 72, prevent the cell holding bottle 73 from falling off during shaking.
The bottom of base 1 is equipped with four support columns 11 that are the rectangle and distribute, carries out the stable stay to base 1 through four support columns 11, prevents that base 1 from appearing rocking, avoids influencing the vibration operation of cell.
The working principle of the invention is as follows: when the invention is used, cell sap is poured into a cell placing bottle 73 manually, a bottle cap is screwed with the front end of the cell placing bottle 73, the cell placing bottle 73 is placed between an upper pressing plate 71 and a lower pressing plate 72 manually, two fastening bolts 74 are rotated to tightly fix the cell placing bottle 73 between the upper pressing plate 71 and the lower pressing plate 72, the cell placing bottle 73 is prevented from falling off during oscillation, then a first driving motor 36 works to drive a semicircular gear 37 fixedly connected with an output shaft of the first driving motor to rotate, the semicircular gear 37 is meshed with a first rack 39, so that the semicircular gear 37 drives an elliptical plate 34 to reciprocate back and forth, the elliptical plate 34 drives a first sliding column 32 and a second sliding column 33 to reciprocate back and forth on two sliding seats 38, the first sliding column 32 drives a push plate 35 to synchronously reciprocate back and forth, the push plate 35 drives a moving plate 2 to reciprocate back and forth on two guide rails 8, the cell liquid in the cell placing component 7 reciprocates back and forth to generate oscillation, meanwhile, the second driving motor 54 works to drive the driving gear 55 fixedly connected with the output shaft of the second driving motor to rotate, because the driving gear 55 is meshed with the driven gear 56, the driving gear 55 drives the driven gear 56 to rotate synchronously, the driven gear 56 drives the rotating shaft 52 to rotate synchronously, the rotating shaft 52 drives the rotating disc 53 to rotate along with the rotating disc, the rotating disc 53 drives the cell placing component 7 to rotate along with the rotating disc, two vibrating motors 57 also work to drive the circular table 51 to vibrate while the second driving motor 54 works, the circular table 51 drives the rotating disc 53 to vibrate along with the rotating shaft 52, the rotating disc 53 drives the cell placing component 7 to vibrate along with the rotating disc, the cell placing component 7 rotates and vibrates, the cell liquid in the cell placing component 7 rotates and vibrates along with the cell liquid, the oscillation is more complete, and meanwhile, the third driving motor 625 works to drive the rotating gear 626 fixedly connected with, because rotating gear 626 meshes with second rack 623, so rotating gear 626 drives sliding plate 622 upwards to move in loading board 621, loading board 621 drives horizontal plate 61 synchronous upwards to move, horizontal plate 61 drives cell placement component 7 also synchronous upwards to move, after cell placement component 7 upwards moved to the certain distance, third driving motor 625 reverse work drives cell placement component 7 downwards to move, with this relapse, cell placement component 7 makes a round trip to reciprocate to go up and down to rock, cell liquid in cell placement component 7 rocks thereupon, aggravate the degree of vibration, the purpose that multiple direction was vibrated to cell liquid has been reached, the effect of vibration is better.
Claims (7)
1. A cell engineering multidimension degree cell oscillation device which characterized in that: comprises a base (1), a moving plate (2), a horizontal driving component (3), a supporting component (4), a rotary vibration component (5), a shaking component (6), a cell placing component (7) and two guide rails (8), wherein the two guide rails (8) are symmetrically arranged on the base (1), the moving plate (2) is arranged on the two guide rails (8), the moving plate (2) is in sliding fit with the two guide rails (8), the horizontal driving component (3) is arranged at the sides of the two guide rails (8), one end of the horizontal driving component (3) is fixedly connected with the moving plate (2), the supporting component (4) is arranged on the supporting component (2), the rotary vibration component (5) is arranged at the top end of the supporting component (4), the shaking component (6) is arranged at the top end of the rotary vibration component (5), the cell placement assembly (7) is mounted on the rocking assembly (6).
2. The cyto-engineered multidimensional cell shaking device of claim 1, wherein: the horizontal driving assembly (3) comprises a vertical plate (31), a first sliding column (32), a second sliding column (33), an elliptical plate (34), a push plate (35), a first driving motor (36), a semicircular gear (37) and two sliding seats (38), wherein the vertical plate (31) is vertically arranged on the base (1), the two sliding seats (38) are symmetrically arranged on the side wall of the vertical plate (31), the first sliding column (32) and the second sliding column (33) are respectively slidably arranged on the two sliding seats (38), two ends of the elliptical plate (34) are respectively fixedly connected with the rear end of the first sliding column (32) and the front end of the second sliding column (33), in addition, first racks (39) are arranged above and below the elliptical plate (34), two ends of the push plate (35) are respectively fixedly connected with the side wall of the movable plate (2) and the front end of the first sliding column (32), the first driving motor (36) is horizontally arranged on the side wall of the vertical plate (31), an output shaft of the first driving motor (36) extends into the elliptical plate (34), the semicircular gear (37) is fixedly connected with the output shaft of the first driving motor (36), and the semicircular gear (37) is meshed with the first rack (39).
3. The cyto-engineered multidimensional cell shaking device of claim 1, wherein: the supporting component (4) comprises a supporting plate (41) and four spring supporting pieces (42), the supporting plate (41) is arranged above the moving plate (2), the spring supporting pieces (42) are distributed on the moving plate (2) in a rectangular mode, the top ends of the spring supporting pieces (42) are fixedly connected with the moving plate (2), each spring supporting piece (42) comprises an upper fixing seat (421), a lower fixing seat (422), a limiting column (423) and a supporting spring (424), the upper fixing seat (421) is fixedly connected with the bottom of the supporting plate (41), the lower fixing seat (422) is fixedly connected with the top of the moving plate (2), the limiting column (423) is arranged in the lower fixing seat (422), the supporting spring (424) is sleeved on the limiting column (423), and the two ends of the supporting spring (424) are respectively connected with the upper fixing seat (421) and the lower fixing seat (422) And (4) fixedly connecting.
4. The cyto-engineered multidimensional cell shaking device of claim 3, wherein: the rotary vibration component (5) comprises a circular table (51), a rotating shaft (52), a rotating disc (53), a second driving motor (54), a driving gear (55), a driven gear (56) and two vibration motors (57), wherein the circular table (51) is vertically arranged on the supporting plate (41), the rotating shaft (52) can be rotatably arranged on the circular table (51), the bottom end of the rotating shaft (52) extends into the circular table (51), the rotating disc (53) is fixedly connected with the top end of the rotating shaft (52), the second driving motor (54) is vertically arranged in the circular table (51), the driving gear (55) is fixedly connected with an output shaft of the second driving motor (54), the driven gear (56) is fixedly connected with the bottom end of the rotating shaft (52), and the driven gear (56) is meshed with the driving gear (55), the two vibration motors (57) are symmetrically arranged on the outer side wall of the circular table (51).
5. The cyto-engineered multidimensional cell shaking device of claim 4, wherein: the swinging assembly (6) comprises a transverse plate (61) and two swinging parts (62), the two swinging parts (62) are symmetrically arranged on the rotating disc (53), two ends of the transverse plate (61) are respectively and fixedly connected with one ends of the two swinging parts (62), each swinging part (62) comprises a bearing plate (621), a sliding plate (622), a second rack (623), a mounting frame (624), a third driving motor (625) and a rotating gear (626), the bearing plate (621) is vertically arranged on the rotating disc (53), an opening is formed in the top end of the bearing plate (621), the sliding plate (622) can be slidably arranged in the bearing plate (621), the side wall of the sliding plate (622) is fixedly connected with one end of the transverse plate (61), and the second rack (623) is fixedly connected with the side wall of the sliding plate (622), the mounting frame (624) is arranged at the side of the bearing plate (621), the third driving motor (625) is horizontally arranged at the top end of the mounting frame (624), the rotating gear (626) is fixedly connected with an output shaft of the third driving motor (625), and the rotating gear (626) is meshed with the second rack (623).
6. The cyto-engineered multidimensional cell shaking device of claim 5, wherein: the cell placing assembly (7) comprises an upper pressing plate (71), a lower pressing plate (72), a cell placing bottle (73), two fastening bolts (74) and four connecting springs (75), wherein the lower pressing plate (72) is fixedly connected with the transverse plate (61), the upper pressing plate (71) is installed on the top of the lower pressing plate (72) through the four connecting springs (75), the cell placing bottle (73) is arranged between the upper pressing plate (71) and the lower pressing plate (72), the two fastening bolts (74) are installed on the upper pressing plate (71) and the lower pressing plate (72), and a bottle cap in threaded connection with the inner wall of the cell placing bottle (73) is arranged at the front end of the cell placing bottle (73).
7. The cyto-engineered multidimensional cell shaking device of claim 1, wherein: the bottom of the base (1) is provided with four support columns (11) which are distributed in a rectangular shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910996924.4A CN110628619A (en) | 2019-10-19 | 2019-10-19 | Cell engineering multidimension degree cell oscillation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910996924.4A CN110628619A (en) | 2019-10-19 | 2019-10-19 | Cell engineering multidimension degree cell oscillation device |
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| Publication Number | Publication Date |
|---|---|
| CN110628619A true CN110628619A (en) | 2019-12-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910996924.4A Withdrawn CN110628619A (en) | 2019-10-19 | 2019-10-19 | Cell engineering multidimension degree cell oscillation device |
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| CN (1) | CN110628619A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197006A (en) * | 2020-01-16 | 2020-05-26 | 杭州键一生物科技有限公司 | CAR-T cell expander |
| CN112337372A (en) * | 2020-10-21 | 2021-02-09 | 南京秉蓬生物科技有限公司 | Shaking mixing device for cell culture and use method |
-
2019
- 2019-10-19 CN CN201910996924.4A patent/CN110628619A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197006A (en) * | 2020-01-16 | 2020-05-26 | 杭州键一生物科技有限公司 | CAR-T cell expander |
| CN111197006B (en) * | 2020-01-16 | 2023-09-12 | 杭州键一生物科技有限公司 | CAR-T cell expander |
| CN112337372A (en) * | 2020-10-21 | 2021-02-09 | 南京秉蓬生物科技有限公司 | Shaking mixing device for cell culture and use method |
| CN112337372B (en) * | 2020-10-21 | 2022-04-12 | 南京秉蓬生物科技有限公司 | Shaking mixing device for cell culture and use method |
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Application publication date: 20191231 |
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| WW01 | Invention patent application withdrawn after publication |