CN110607327B - Preparation method of rice wine vinasse extract - Google Patents
Preparation method of rice wine vinasse extract Download PDFInfo
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- CN110607327B CN110607327B CN201910989323.0A CN201910989323A CN110607327B CN 110607327 B CN110607327 B CN 110607327B CN 201910989323 A CN201910989323 A CN 201910989323A CN 110607327 B CN110607327 B CN 110607327B
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Abstract
The invention discloses a preparation method of rice wine lees extract, which adds an XDA-A adsorption resin when preparing the rice wine lees extract, the resin realizes the adsorption of fusel oil in the rice wine lees extract by van der Waals force of active tips on the porous surface of the resin, further removes the fusel oil, and simultaneously adds acetaldehyde dehydrogenase, lees can generate a large amount of aldehydes when fermenting, wherein, most acetaldehyde is occupied by the acetaldehyde, the acetaldehyde dehydrogenase decomposes the acetaldehyde in the rice wine lees extract into harmless acetic acid by utilizing the self specificity, simultaneously, the acetic acid has the advantages of expanding blood vessels, being beneficial to reducing blood pressure, preventing cardiovascular diseases and the like, and an oscillating distillation device is used, so that in the process of preparing the extract, the device does not need to be replaced for many times, the extract is ensured not to be polluted, the extraction efficiency is improved, and the shock absorption rod is additionally arranged, so that the service life of the equipment is further prolonged.
Description
Technical Field
The invention belongs to the field of vinasse extraction, and particularly relates to a preparation method of a rice wine vinasse extract.
Background
The rice wine contains more than ten amino acids, 8 of the amino acids are necessary and cannot be synthesized by a human body, the lysine content in each liter of rice wine is several times higher than that of wine and beer, and the lysine content is rare in other nutritional wines, so that the rice wine is called as a liquid cake, the nutritional ingredients in the rice wine are easy to absorb by the human body, and the rice wine is a good product for tonifying qi and nourishing blood for middle-aged and old people, pregnant women and weak people;
the invention CN103757084A discloses a preparation method of a rice wine lees extract, which comprises the following steps of (1) rice wine lees secondary fermentation: adding water into the rice wine vinasse for fermentation to obtain feed liquid 1; (2) enzymolysis: adding protease and glycoside hydrolase into the feed liquid 1 for enzymolysis, and filtering to obtain feed liquid 2; (3) purification and concentration: decolorizing the feed liquid 2, deodorizing, filtering to obtain feed liquid 3, and concentrating to obtain rice wine lees extract; the invention solves the problem of environmental pollution caused by directly discarding the rice wine vinasse, but the invention does not separate toxic and harmful substances generated by secondary fermentation of the rice wine vinasse when preparing the rice wine vinasse extract, so that partial harmful substances are remained in the extract, the extraction amount of amino acid is not high, and the preparation efficiency is not high.
Disclosure of Invention
The invention aims to provide a preparation method of rice wine vinasse extract, which adds an XDA-A adsorption resin when preparing the rice wine vinasse extract, wherein the resin realizes the adsorption of fusel oil in the rice wine vinasse extract through van der Waals force of active tips on the porous surface of the resin, further removes the fusel oil, and simultaneously adds acetaldehyde dehydrogenase, vinasse can generate a large amount of aldehydes when in fermentation, wherein the acetaldehyde occupies most acetaldehyde, the acetaldehyde dehydrogenase utilizes the specificity of the acetaldehyde dehydrogenase to decompose the acetaldehyde in the rice wine vinasse extract into harmless acetic acid, and the acetic acid has various advantages of expanding blood vessels, being beneficial to reducing blood pressure, preventing cardiovascular diseases and the like, and an oscillation distillation device is used, so that equipment does not need to be replaced for many times in the process of preparing the extract, and the extract is ensured not to be polluted, the extraction efficiency is improved, the shock absorption rod is additionally arranged, and the service life of the equipment is further prolonged.
The technical problems to be solved by the invention are as follows:
1. in the process of extracting beneficial substances from rice wine vinasse by the traditional process, the extracted extract contains a large amount of fusel oil, the fusel oil is a fatty alcohol mixture with carbon number more than two, the content of the fusel oil is too high, the fusel oil has toxic action on people, the poisoning and anesthesia effects of the fusel oil are stronger than that of ethanol, the nervous system can be congested, the headache of people can be caused, the toxicity of the fusel oil is aggravated along with the increase of molecular weight, the oxidation speed of the fusel oil in the body is slower than that of the ethanol, and the retention time in the body;
2. in the process of extracting beneficial substances from rice wine vinasse by the traditional process, the extracted extract contains a large amount of aldehydes, wherein the proportion of acetaldehyde is the largest, low-concentration acetaldehyde causes eye, nose and upper respiratory tract stimulation symptoms and bronchitis, high-concentration acetaldehyde has an anesthetic effect after being inhaled, shows headache, somnolence, obnubilation, bronchitis, pulmonary edema, diarrhea, proteinuria liver and myocardial steatosis, can cause death, and causes gastrointestinal tract stimulation symptoms, anesthetic effect and heart, liver and kidney damage after being taken by mistake. Has skin sensitization, and can cause dermatitis and conjunctivitis due to repeated steam exposure;
3. in the process of extracting the beneficial substances from the rice wine vinasse by the traditional process, the extracted beneficial substances are not high, and a large amount of beneficial substances are lost.
The purpose of the invention can be realized by the following technical scheme:
a preparation method of a rice wine vinasse extract comprises the following specific steps:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting at 20-30 deg.C for 24 hr to obtain a mixture, adding the mixture into a filter, and filtering lees residue to obtain a fermentation liquid;
step S2: adding the adsorption resin into a Soxhlet extractor, extracting the adsorption resin for 8h by using acetone as a solvent, taking out the extracted adsorption resin, washing the adsorption resin for 3-5min by using deionized water, putting the adsorption resin into an oven after cleaning, and drying for 30-40min at the temperature of 60-70 ℃ to obtain the dried adsorption resin;
step S3: adding the fermentation liquor obtained in the step S1 and the adsorption resin obtained in the step S2 into a vibration distillation device, vibrating for 6-8 hours at the temperature of 15-20 ℃ to obtain a mixed solution 1, and filtering the mixed solution 1 to obtain an extracting solution 1;
step S4: adding enzyme into the shaking distillation device containing the extractive solution 1 in step S3, reacting at 25-35 deg.C for 30-45min, distilling at 125 deg.C and 120 deg.C, and removing distillate to obtain rice wine lees extract.
Furthermore, the dosage of the distilled water is 20mL/g rice wine vinasse.
Furthermore, the adsorption resin is XDA-A adsorption resin, and the dosage of the adsorption resin is 20g/L fermentation liquor.
Further, the enzyme is acetaldehyde dehydrogenase, the enzyme activity is 150 ten thousand U/g, and the dosage of the enzyme is 5g/L of the extracting solution 1.
Further, the oscillation distillation device in the step S3 includes a base, an oscillation device, a rubber hose, a distillation device, and a condenser pipe, the base is placed on a horizontal plane, a support frame is provided on an upper end surface of the base, a support column is provided at one end of the base, a shock-absorbing rod is provided on the support column, a main fixed cross beam is provided at a top end of the support column, the main fixed cross beam is provided with the shock-absorbing rod, and a connecting chain is further provided on the main fixed cross beam;
the vibrating device is positioned on the support frame, the heating bin and the vibrating bin are arranged inside the vibrating device, the bottom end of the vibrating device is provided with a vibrating discharge port, a first sealing valve is arranged inside the vibrating discharge port, the lower end inside the vibrating discharge port is provided with a filter screen, the top end of the vibrating device is provided with a sealing end cover, the sealing end cover is fixed on the vibrating device through screw threads, a heating rod is arranged on the sealing end cover, the sealing end cover is fixedly connected with the connecting chain, and one side of the outside of the vibrating device is provided with an oscillator;
distillation plant passes through flexible rubber hose and is connected with oscillation device, distillation plant includes the distillation storehouse, open respectively on the top in distillation storehouse has the feed inlet, the charge door, the distillation mouth, be equipped with the sealed valve of second in the feed inlet, be equipped with the sealed valve of third in the charge door, be equipped with the sealed valve of fourth in the distillation mouth, the bottom in distillation storehouse is the toper, open the bottom in distillation storehouse has the discharge gate, be equipped with the sealed valve of fourth in the discharge gate, outside one side in distillation storehouse is equipped with the heater, distillation mouth and condenser pipe fixed connection, be equipped with heat exchanger on the condenser pipe.
Furthermore, the upper end face of the base is also provided with a material collecting groove and a distillation collecting groove, the material collecting groove is positioned under the discharge port, the distillation collecting groove is positioned under the pipe orifice of the condensation pipe, the number of the supporting frames is two, one end of each supporting frame is fixedly connected with the upper end face of the base, the other end of each supporting frame is fixedly connected with the bottom end of the distillation device, the main fixing cross beam is L-shaped and comprises a horizontal section and a vertical section, the horizontal section of the main fixing cross beam is vertical to the supporting column, the vertical section of the main fixing cross beam is positioned under the horizontal section of the main fixing cross beam, the connecting chain is positioned at the center of the lower end face of the horizontal section of the main fixing cross beam, the center of two sides of the horizontal section of the main fixing cross beam is provided with an auxiliary fixing cross beam, the auxiliary fixing cross beam is L-shaped and comprises a horizontal section and a, the auxiliary fixed cross beam vertical section is located below the auxiliary fixed cross beam horizontal section, the damping rods are arranged at the center positions of the main fixed cross beam vertical section and the auxiliary fixed cross beam vertical section, and the damping rods are located on the same horizontal plane.
Further, one end of the connecting chain is fixedly connected with the main fixed cross beam, the other end of the connecting chain is fixedly connected with the center of the sealing end cover, a thermometer is arranged on the sealing end cover, the heating bin and the oscillating bin are two independent areas, the heating bin wraps the oscillating bin, the bottom end of the oscillating device is conical, the caliber of the oscillating discharge port is the same as the diameter of the oscillating bin, the oscillating bin is communicated with the discharge port, the heating bin is not communicated with the discharge port, the number of the heating rods is two, and the heating rods are located inside the heating bin.
Furthermore, one end of the rubber hose is fixedly connected with the oscillating discharge port, and the other end of the rubber hose is fixedly connected with the feed port.
The invention has the beneficial effects that:
(1) the invention adds an XDA-A adsorption resin when preparing the rice wine vinasse extract, the resin realizes the adsorption of fusel oil in the rice wine vinasse extract by Van der Waals force of active tips on the porous surface of the resin, further removes the fusel oil, and simultaneously adds acetaldehyde dehydrogenase, the vinasse can generate a large amount of aldehydes when in fermentation, wherein, most acetaldehyde is occupied, the acetaldehyde dehydrogenase decomposes the acetaldehyde in the rice wine vinasse extract into harmless acetic acid by utilizing the self specificity, and the acetic acid has the advantages of expanding blood vessels, being beneficial to reducing blood pressure, preventing cardiovascular diseases and the like;
(2) the invention uses an oscillating distillation device when preparing the rice wine vinasse extract, the oscillating distillation device comprises a base, an oscillating device, a rubber hose, a distillation device and a condenser pipe, the inside of the oscillating device is provided with a heating bin and an oscillating bin, one side of the outside of the oscillating device is provided with an oscillator, so that the material can enter the oscillating bin to oscillate and can be heated in a water area, the rubber hose and the distillation device are mutually connected, the device does not need to be replaced and used repeatedly in the process of preparing the extract, the extract is ensured not to be polluted, the possibility of material loss caused by transfer equipment is reduced, the extraction efficiency is improved, the upper end of the oscillating device is fixed by a connecting chain, the oscillating device can freely oscillate, the rubber hose ensures that the oscillating device does not influence the distillation device when oscillating, and the service life of the device is prolonged, the shock absorption rod is additionally arranged to limit the oscillation range of the oscillation device, the force generated by oscillation is offset, the normal work of the oscillation device is guaranteed, meanwhile, equipment cannot be damaged due to the fact that the oscillation amplitude is too large, and the service life of the equipment is further prolonged.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view showing the structure of a shaking and distilling apparatus in a method for preparing a rice wine lees extract according to the present invention;
FIG. 2 is a top view of a main fixed beam and an auxiliary fixed beam in a shaking and distilling apparatus in the method for preparing a rice wine lees extract according to the present invention;
FIG. 3 is a schematic view showing the structure of a shaking unit in a shaking distillation apparatus in the method for preparing a rice wine lees extract according to the present invention;
FIG. 4 is a plan view of an oscillation device in the oscillation distillation device in the method for producing a rice wine lees extract according to the present invention;
FIG. 5 is a schematic view showing the structure of a distillation apparatus in a shaking distillation apparatus in the method for preparing a rice wine lees extract according to the present invention.
In the figure: 1. a base; 11. a support frame; 12. a support pillar; 13. a main fixed cross beam; 14. a secondary fixed cross beam; 15. a connecting chain; 16. a shock-absorbing lever; 2. an oscillation device; 21. a heating chamber; 22. an oscillation bin; 23. oscillating the discharge hole; 231. a first sealing valve; 232. a filter screen; 24. sealing the end cap; 241. a heating rod; 242. a thermometer; 25. an oscillator; 3. a rubber hose; 4. a distillation apparatus; 41. a distillation bin; 42. a feed inlet; 421. a second sealing valve; 43. a feed inlet; 431. a third sealing valve; 44. a distillation opening; 45. a heater; 46. a discharge port; 461. a fourth seal valve; 5. a condenser tube; 51. a heat exchanger; 6. a material collecting groove; 7. and (5) distilling the collecting tank.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a rice wine vinasse extract comprises the following steps:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting for 24h at 20 ℃ to obtain a mixture, adding the mixture into a filter, and filtering lees residues to obtain a fermentation liquid;
step S2: adding XDA-A adsorbent resin into a Soxhlet extractor, extracting XDA-A adsorbent resin for 8h by using acetone as a solvent, taking out the extracted XDA-A adsorbent resin, washing the XDA-A adsorbent resin for 3min by using deionized water, putting the XDA-A adsorbent resin into an oven after washing, and drying for 30min at the temperature of 60 ℃ to obtain the dried XDA-A adsorbent resin;
step S3: adding the fermentation liquid obtained in the step S1 and the XDA-A adsorption resin obtained in the step S2 into a shaking and distilling device, shaking for 6 hours at the temperature of 15 ℃ to obtain a mixed solution 1, and filtering the mixed solution 1 to obtain an extracting solution 1;
step S4: adding acetaldehyde dehydrogenase into the shaking and distilling device containing the extractive solution 1 in step S3, reacting at 25 deg.C for 30min, distilling at 120 deg.C, and removing distillate to obtain rice wine lees extract.
Example 2
A preparation method of a rice wine vinasse extract comprises the following steps:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting for 24h at the temperature of 30 ℃ to obtain a mixture, adding the mixture into a filter, and filtering lees residues to obtain a fermentation liquid;
step S2: adding XDA-A adsorbent resin into a Soxhlet extractor, extracting XDA-A adsorbent resin for 8h by using acetone as a solvent, taking out the extracted XDA-A adsorbent resin, washing the XDA-A adsorbent resin for 5min by using deionized water, putting the XDA-A adsorbent resin into an oven after washing, and drying for 40min at the temperature of 70 ℃ to obtain the dried XDA-A adsorbent resin;
step S3: adding the fermentation liquid obtained in the step S1 and the XDA-A adsorption resin obtained in the step S2 into a shaking and distilling device, shaking for 8 hours at the temperature of 20 ℃ to obtain a mixed solution 1, and filtering the mixed solution 1 to obtain an extracting solution 1;
step S4: adding acetaldehyde dehydrogenase into the shaking and distilling device containing the extractive solution 1 in step S3, reacting at 35 deg.C for 45min, distilling at 125 deg.C, and removing distillate to obtain rice wine lees extract.
Comparative example 1
This comparative example was prepared by replacing the adsorbent resin in step S2 with activated carbon as compared with example 1, specifically by the following method:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting for 24h at 20 ℃ to obtain a mixture, adding the mixture into a filter, and filtering lees residues to obtain a fermentation liquid;
step S2: soaking granular activated carbon in distilled water for 24h, then putting the activated carbon into a drying oven, and drying for 30min at the temperature of 110 ℃ to obtain dried activated carbon;
step S3: adding the fermentation liquor obtained in the step S1 and the activated carbon obtained in the step S2 into a vibration distillation device, vibrating for 6 hours at the temperature of 15-20 ℃ to obtain a mixed solution 2, and filtering the mixed solution 2 to obtain an extracting solution 2;
step S4: adding acetaldehyde dehydrogenase into the shaking and distilling device containing the extractive solution 2 in step S3, reacting at 25 deg.C for 30min, distilling at 120 deg.C, and removing distillate to obtain rice wine lees extract.
Comparative example 2
This comparative example was prepared without using the oscillating distillation apparatus in step S3, as compared with example 1, by the following method:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting for 24h at 20 ℃ to obtain a mixture, adding the mixture into a filter, and filtering lees residues to obtain a fermentation liquid;
step S2: adding XDA-A adsorbent resin into a Soxhlet extractor, extracting XDA-A adsorbent resin for 8h by using acetone as a solvent, removing the XDA-A adsorbent resin after extraction, washing the XDA-A adsorbent resin for 3min by using deionized water, putting the XDA-A adsorbent resin into an oven after washing, and drying for 30min at the temperature of 60 ℃ to obtain the dried XDA-A adsorbent resin;
step S3: adding the fermentation liquid obtained in the step S1 and the XDA-A adsorption resin obtained in the step S2 into an oscillator, oscillating for 6 hours at the temperature of 15 ℃ to obtain a mixed solution 3, and filtering the mixed solution 3 to obtain an extracting solution 3;
step S4: adding the extractive solution 3 and acetaldehyde dehydrogenase prepared in step S3 into a reaction kettle, reacting at 25 deg.C for 30min, distilling at 120 deg.C, and removing distillate to obtain rice wine lees extract.
Comparative example 3
This comparative example was prepared without extraction using the extraction method of step S2 and step S3, as compared to example 1, by the following method:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting at 20-30 deg.C for 24 hr to obtain a mixture, adding the mixture into a filter, and filtering lees residue to obtain a fermentation liquid;
step S2: adding the fermentation liquor prepared in the step S1 into a distillation still, distilling for 3min at the temperature of 78 ℃, removing the distillate, continuing to distill for 10min at the temperature of 78 ℃ to obtain an extracting solution 4, and removing the tail solution;
step S3: adding the extract 4 obtained in step S2 into a distillation still, distilling at 120 deg.C, and removing distillate to obtain rice wine lees extract.
The rice wine extracts prepared in examples 1-2 and comparative examples 1-3 were tested for fusel oil content, acetaldehyde content, and amino acid content, and the test results are shown in table 1 below;
fusel oil content: and detecting according to the GB/T10345.1-1989 standard to obtain the fusel oil content.
Acetaldehyde content: adding 5g of the rice wine lees extracts prepared in the examples 1-2 and the comparative examples 1-3 into a 25mL graduated test tube, sequentially adding 5mL of absolute ethyl alcohol, 1mL of 2, 4-dinitrophenylhydrazine solution and 0.05mL of 12mol/L hydrochloric acid, covering a bottle stopper, heating in a water area at 50 ℃ for 30min, taking out, cooling to room temperature, adding 5.0mL of 100g/L potassium hydroxide-ethanol solution, standing for 5min, adding 5mL of water for dilution, adding the diluted solution into a cuvette, measuring absorbance at a wavelength of 445nm, and further calculating the acetaldehyde content.
Amino acid content: adding 5g of the rice wine lees extracts prepared in examples 1-2 and comparative examples 1-3 into a 25mL graduated test tube, adding 5mL of water, shaking up, adding 1mL of 2mol/L acetic acid buffer solution with pH5.4 and 1mL of ninhydrin color development solution, mixing uniformly, heating in a boiling water area at 100 ℃ for 15min, cooling with tap water, standing for 5min, adding 3mL of 60% ethanol for dilution, shaking up, adding the diluted solution into a cuvette, measuring absorbance at a wavelength of 570nm, and further calculating the amino acid content.
TABLE 1
As can be seen from Table 1 above, the fusel oil contents of the rice wine lees extracts prepared in examples 1-2 and comparative example 2 are much smaller than those of comparative example 1 and comparative example 3; the acetaldehyde content of the rice wine lees extracts prepared in examples 1-2 and comparative examples 1-2 was much less than that of comparative example 3; the content of amino acids in the rice wine lees extract prepared in the example 1-2 is much higher than that in the comparative example 1-3; therefore, the rice wine vinasse extract prepared by the preparation method of the rice wine vinasse extract has low content of fusel oil, acetaldehyde and other toxic and harmful substances, high content of amino acid, more beneficial components in the extract and high extraction efficiency.
Referring to fig. 1-5, the oscillating distillation apparatus described in step S3 includes a base 1, an oscillating device 2, a rubber hose 3, a distillation apparatus 4, and a condenser tube 5, wherein the base 1 is placed on a horizontal plane, a support frame 11 is disposed on an upper end surface of the base 1, a support post 12 is disposed at one end of the base 1, a shock-absorbing rod 16 is disposed on the support post 12, a main fixed cross beam 13 is disposed at a top end of the support post 12, the main fixed cross beam 13 is disposed with the shock-absorbing rod 16, and a connecting chain 15 is further disposed on the main fixed cross beam 13;
the oscillating device 2 is positioned on the support frame 11, the heating bin 21 and the oscillating bin 22 are arranged inside the oscillating device 2, the bottom end of the oscillating device 2 is provided with an oscillating discharge hole 23, the upper end inside the oscillating discharge hole 23 is provided with a first sealing valve 231, the lower end inside the oscillating discharge hole 23 is provided with a filter screen 232, the top end of the oscillating device 2 is provided with a sealing end cover 24, the sealing end cover 24 is fixed on the oscillating device 2 through screw threads, the sealing end cover 24 is provided with a heating rod 241, the sealing end cover 24 is fixedly connected with the connecting chain 15, and one side of the outside of the oscillating device 2 is provided;
The upper end face of the base 1 is also provided with a material collecting groove 6 and a distillation collecting groove 7, the material collecting groove 6 is positioned under the discharge port 46, the distillation collecting groove 7 is positioned under the pipe orifice of the condenser pipe 5, the number of the support frames 11 is two, one end of each support frame 11 is fixedly connected with the upper end face of the base 1, the other end of each support frame 11 is fixedly connected with the bottom end of the distillation device 4, the main fixed cross beam 13 is L-shaped, the main fixed cross beam 13 comprises a horizontal section and a vertical section, the horizontal section of the main fixed cross beam 13 is vertical to the support column 12, the vertical section of the main fixed cross beam 13 is positioned under the horizontal section of the main fixed cross beam 13, the connecting chain 15 is positioned at the center position of the lower end face of the horizontal section of the main fixed cross beam 13, the center positions of two sides of the horizontal section of the main fixed cross beam 13 are provided with auxiliary fixed cross beams 14, the auxiliary fixed cross beam 14 is L-shaped, the, the vertical section of the auxiliary fixed cross beam 14 is positioned below the horizontal section of the auxiliary fixed cross beam 14, the central positions of the vertical section of the main fixed cross beam 13 and the vertical section of the auxiliary fixed cross beam 14 are both provided with shock absorption rods 16, and the shock absorption rods 16 are positioned on the same horizontal plane.
One end of a connecting chain 15 is fixedly connected with the main fixed cross beam 13, the other end of the connecting chain 15 is fixedly connected with the center of a sealing end cover 24, a thermometer 242 is arranged on the sealing end cover 24, the heating bin 21 and the oscillating bin 22 are two independent areas, the heating bin 21 wraps the oscillating bin 22, the bottom end of the oscillating device 2 is conical, the caliber of the oscillating discharge hole 23 is the same as the diameter of the oscillating bin 22, the oscillating bin 22 is communicated with the oscillating discharge hole 23, the heating bin 21 is not communicated with the oscillating discharge hole 23, the number of the heating rods 241 is two, and the heating rods 241 are located inside the heating bin 21.
One end of the rubber hose 3 is fixedly connected with the oscillation discharge port 23, and the other end of the rubber hose 3 is fixedly connected with the feed port 42.
The working principle is as follows: the first sealing valve 231 and the fourth sealing valve 461 are closed, the fermentation liquid and the adsorption resin are added into the oscillating bin 22, the heating chamber 21 is filled with water, the end cap 24 is sealed, the heating rod 241 and the oscillator 25 are opened, observe the temperature of the water area through the thermometer 242, further adjust the heating rod 241 to the required temperature, open the first sealing valve 231 after finishing oscillating, the mixed liquor is filtered through the filter screen 232 along the oscillating discharge port 23, get the extract 1 and get into the distillation apparatus 4 from the feed inlet 42, add the enzyme into the distillation apparatus 4 through the feed inlet 43, close the second sealing valve 421 and the third sealing valve 431, open the heater 45 and set the designated temperature, operate the heat exchanger 51, the extract 1 is distilled, the distillate enters the condenser pipe 5 and condenses into the liquid state and gets into the distillation collecting tank 7 to collect, open the fourth sealing valve 461, use the collecting tank 6 to collect the rice wine lees extract.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (4)
1. A preparation method of a rice wine vinasse extract is characterized by comprising the following steps: the method comprises the following steps:
step S1: adding rice wine lees and distilled water into a reaction kettle, fermenting at 20-30 deg.C for 24 hr to obtain a mixture, adding the mixture into a filter, and filtering lees residue to obtain a fermentation liquid;
step S2: adding the adsorption resin into a Soxhlet extractor, extracting the adsorption resin for 8h by using acetone as a solvent, taking out the extracted adsorption resin, washing the adsorption resin for 3-5min by using deionized water, putting the adsorption resin into an oven after cleaning, and drying for 30-40min at the temperature of 60-70 ℃ to obtain the dried adsorption resin;
step S3: adding the fermentation liquor obtained in the step S1 and the adsorption resin obtained in the step S2 into a vibration distillation device, vibrating for 6-8 hours at the temperature of 15-20 ℃ to obtain a mixed solution 1, and filtering the mixed solution 1 to obtain an extracting solution 1;
step S4: adding enzyme into the oscillatory distillation device containing the extracting solution 1 in the step S3, reacting at 25-35 deg.C for 30-45min, distilling at 125 deg.C and 120 deg.C, and removing distillate to obtain rice wine lees extract;
the vibration distillation device comprises a base (1), a vibration device (2), a rubber hose (3), a distillation device (4) and a condensation pipe (5), wherein the base (1) is placed on a horizontal plane, a support frame (11) is arranged on the upper end face of the base (1), a support column (12) is arranged at one end of the base (1), a shock absorption rod (16) is arranged on the support column (12), a main fixing cross beam (13) is arranged at the top end of the support column (12), the main fixing cross beam (13) is provided with the shock absorption rod (16), and a connecting chain (15) is further arranged on the main fixing cross beam (13);
the vibration device (2) is positioned on the support frame (11), the heating bin (21) and the vibration bin (22) are arranged inside the vibration device (2), the bottom end of the vibration device (2) is provided with a vibration discharge hole (23), a first sealing valve (231) is arranged inside the vibration discharge hole (23), the lower end inside the vibration discharge hole (23) is provided with a filter screen (232), the top end of the vibration device (2) is provided with a sealing end cover (24), the sealing end cover (24) is fixed on the vibration device (2) through screwing, the sealing end cover (24) is provided with a heating rod (241), the sealing end cover (24) is fixedly connected with the connecting chain (15), and one side of the outside of the vibration device (2) is provided with an oscillator (25);
the distillation device (4) is connected with the oscillation device (2) through a rubber hose (3), the distillation device (4) comprises a distillation bin (41), a feed inlet (42), a feed inlet (43) and a distillation opening (44) are formed in the top end of the distillation bin (41) respectively, a second sealing valve (421) is arranged in the feed inlet (42), a third sealing valve (431) is arranged in the feed inlet (43), the bottom end of the distillation bin (41) is conical, a discharge outlet (46) is formed in the bottom end of the distillation bin (41), a fourth sealing valve (461) is arranged in the discharge outlet (46), a heater (45) is arranged on one side of the outer portion of the distillation bin (41), the distillation opening (44) is fixedly connected with a condensation pipe (5), and a heat exchanger (51) is arranged on the condensation pipe (5;
the upper end face of the base (1) is further provided with a material collecting groove (6) and a distillation collecting groove (7), the material collecting groove (6) is located under the discharge port (46), the distillation collecting groove (7) is located under the pipe orifice of the condenser pipe (5), the number of the support frames (11) is two, one end of each support frame (11) is fixedly connected with the upper end face of the base (1), the other end of each support frame (11) is fixedly connected with the bottom end of the distillation device (4), each main fixing cross beam (13) is L-shaped, each main fixing cross beam (13) comprises a horizontal section and a vertical section, the horizontal section of each main fixing cross beam (13) is perpendicular to the support columns (12), the vertical section of each main fixing cross beam (13) is located under the horizontal section of the corresponding main fixing cross beam (13), the connecting chain (15) is located at the center position of the lower end face of the horizontal section of the corresponding main fixing cross beam (13), and auxiliary fixing cross beams (14) are arranged at the, the auxiliary fixed cross beam (14) is L-shaped, the auxiliary fixed cross beam (14) comprises a horizontal section and a vertical section, the horizontal section of the auxiliary fixed cross beam (14) is vertical to the horizontal section of the main fixed cross beam (13), the vertical section of the auxiliary fixed cross beam (14) is positioned below the horizontal section of the auxiliary fixed cross beam (14), the central positions of the vertical section of the main fixed cross beam (13) and the vertical section of the auxiliary fixed cross beam (14) are both provided with shock absorption rods (16), and the shock absorption rods (16) are positioned on the same horizontal plane;
one end of the connecting chain (15) is fixedly connected with the main fixed cross beam (13), the other end of the connecting chain (15) is fixedly connected with the center of the sealing end cover (24), a thermometer (242) is arranged on the sealing end cover (24), the heating bin (21) and the oscillating bin (22) are two independent areas, the heating bin (21) wraps the oscillating bin (22), the bottom end of the oscillating device (2) is conical, the caliber of the oscillating discharge port (23) is the same as the diameter of the oscillating bin (22), the oscillating bin (22) is communicated with the oscillating discharge port (23), the heating bin (21) is not communicated with the oscillating discharge port (23), the number of the heating rods (241) is two, and the heating rods (241) are positioned in the heating bin (21);
one end of the rubber hose (3) is fixedly connected with the oscillating discharge port (23), and the other end of the rubber hose (3) is fixedly connected with the feed port (42);
the enzyme is acetaldehyde dehydrogenase.
2. The method of claim 1, wherein the extract of distiller's grains of rice wine is prepared by: the dosage of the distilled water is 20mL/g rice wine vinasse.
3. The method of claim 1, wherein the extract of distiller's grains of rice wine is prepared by: the adsorption resin is XDA-A adsorption resin, and the dosage of the adsorption resin is 20g/L fermentation liquor.
4. The method of claim 1, wherein the extract of distiller's grains of rice wine is prepared by: the enzyme activity of the acetaldehyde dehydrogenase is 150 ten thousand U/g, and the dosage of the enzyme is 5g/L extracting solution 1.
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