CN110563831A - Separation device capable of realizing preparation of multiple N-telopeptide precursor recombinant proteins and use method - Google Patents
Separation device capable of realizing preparation of multiple N-telopeptide precursor recombinant proteins and use method Download PDFInfo
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- CN110563831A CN110563831A CN201910801045.1A CN201910801045A CN110563831A CN 110563831 A CN110563831 A CN 110563831A CN 201910801045 A CN201910801045 A CN 201910801045A CN 110563831 A CN110563831 A CN 110563831A
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
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Abstract
the invention discloses a separation device capable of realizing preparation of a plurality of N-telopeptide precursor recombinant proteins and a use method thereof. According to the reagent bottle fixing device, the clamping device is arranged, the threaded rod is rotated to drive the inserting plate to move in the third sliding groove, so that the bottom plate is in contact with the reagent bottle, the push plate is pushed to drive the clamping plate to move, the reagent bottle is clamped by the clamping plate, the reagent bottle fixing device can adapt to the fixing operation of reagent bottles with different diameters, and the universality of the reagent bottle fixing device is greatly improved; the rotating plate is arranged, the rotating device drives the rotating plate to rotate, so that the round rod drives the arc-shaped plate to move, the roller wheels clamp the reagent bottle, the stability of the reagent bottle in the centrifugal protein preparation process is effectively improved, and the working efficiency of the device is greatly improved by arranging the plurality of through holes and the clamping device; through being provided with the slipmat, the effectual damage to the reagent bottle of having avoided. The invention has the advantages of high working efficiency and good universality.
Description
Technical Field
the invention relates to the technical field of preparation of N-telencephalon natriuretic peptide precursors, in particular to a separation device capable of realizing preparation of a plurality of N-telencephalon natriuretic peptide precursor recombinant proteins and a use method thereof.
background
The N-terminal pro-brain natriuretic peptide is an inactive N-terminal fragment of BNP prohormone after cleavage, has a longer half-life and is more stable than BNP, and its concentration reflects the release of BNP newly synthesized rather than stored in a short time, and thus more reflects the activation of the BNP pathway. Plasma N-telopeptide precursor levels increase with increasing degree of heart failure. The sensitivity and specificity of the adult plasma N-telencephalon precursor concentration of less than 50 years old to diagnosing acute heart failure of 450pg/ml are 93 percent and 95 percent respectively; the sensitivity and specificity of 900pg/ml in the diagnosis of heart failure in human plasma concentrations above 50 years of age were 91% and 80%, respectively. The heart failure can be well diagnosed by measuring the content of the N-telencephalon natriuretic peptide precursor, and the N-telencephalon natriuretic peptide precursor is an important index for judging the heart failure in medicine.
in the prior art, the preparation of the N-terminal brain natriuretic peptide precursor recombinant protein adopts gene recombination and then synthesizes the N-terminal brain natriuretic peptide precursor recombinant protein, the purity of the N-terminal brain natriuretic peptide precursor recombinant protein prepared by the preparation method is not high, and centrifugal operation needs to be carried out on solution by using centrifugal equipment to achieve the purpose of purification, most of the N-terminal brain natriuretic peptide precursor recombinant protein centrifugal equipment in the prior art can only carry out centrifugal purification operation on a single reagent bottle at one time, the operation efficiency is very low, the expansion of the production scale of the N-terminal brain natriuretic peptide precursor recombinant protein is not facilitated, and the N-terminal brain natriuretic peptide precursor recombinant protein is a great disadvantage in the prior art; in addition, the prior art centrifugation device can only fix a reagent bottle with a single diameter, and has poor universality, and in the actual culture process, if the reagent bottle is replaced for many times, the N-terminal brain natriuretic peptide precursor recombinant protein can be polluted to a certain extent, and the culture operation is not facilitated, which is another deficiency of the prior art. For the above reasons, the present invention proposes a separation device that can achieve the production of a plurality of N-terminal natriuretic peptide precursor recombinant proteins to solve these deficiencies.
disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of low operation efficiency and poor universality in the prior art, and provide a separation device capable of realizing preparation of a plurality of N-terminal brain natriuretic peptide precursor recombinant proteins and a using method thereof. The separation device capable of realizing preparation of a plurality of N-telopeptide precursor recombinant proteins and the use method thereof have the characteristics of high working efficiency, good universality and the like.
In order to achieve the purpose, the invention provides the following technical scheme: a separation device capable of realizing preparation of a plurality of N-terminal brain natriuretic peptide precursor recombinant proteins comprises a shell, wherein the shell is arranged in a circular shape, a circular plate is fixedly connected to the position, close to the top surface, of an inner cavity of the shell, a plurality of through holes are uniformly distributed on the circular plate and penetrate through the upper side wall and the lower side wall of the circular plate, transverse plates are fixedly connected to the two sides, located at the through holes, of the top surface of the circular plate, two arc-shaped plates are connected between the two transverse plates, arc-shaped surfaces of the arc-shaped plates are arranged towards one side of the center of the through holes, two idler wheels are symmetrically and movably connected to the arc-shaped surfaces of the two arc-shaped plates, a circular rod is fixedly connected to the center of the straight surface of the arc-shaped plate, close to one side of the center of the circular plate, a flat, the round bar is sleeved with a first spring, one end of the first spring is fixedly connected to the flat plate, the other end of the first spring is fixedly connected to the vertical plate, the top surface center of the circular plate is provided with a rotating plate, one ends of the round bars, far away from the arc-shaped plate, are movably connected with the rotating plate, the center of the rotating plate is provided with a rotating device, the inner cavity of the shell is positioned at the lower side of the circular plate and is provided with a movable plate, the movable plate is movably connected with the shell, the bottom surface center of the movable plate is fixedly connected with a motor, a rotor of the motor is fixedly connected with a main shaft, one end of the main shaft, far away from the motor, penetrates through the movable plate and is fixedly connected with a first gear, the main shaft is movably connected with the movable plate, the top surface of the movable plate is uniformly and movably connected with, and a clamping device is arranged on the second gear.
Preferably, the clamping device comprises rectangular grooves, four rectangular grooves are uniformly distributed on the second gear, the rectangular groove is arranged through the upper side wall and the lower side wall of the second gear, a clamping plate is arranged in the rectangular groove, the side wall of the clamping plate positioned in the inner cavity of the rectangular groove is fixedly connected with a round shaft, the two ends of the round shaft are movably connected with the side wall of the rectangular groove, the movable plate is provided with a circular groove at the lower side of the second gear, the bottom surfaces of the clamping plates are inserted into the circular groove, push plates are arranged among the four clamping plates, the push plate and the clamping plate are movably connected, a second spring is fixedly connected on the bottom surface of the push plate, the other end of the second spring is fixedly connected on the bottom surface of the circular groove, the center of the top surface of the push plate is fixedly connected with a vertical rod, one end, far away from the push plate, of the vertical rod penetrates through the bottom plate fixedly connected with the second gear, and the vertical rod is movably connected with the second gear.
Preferably, rotating device includes the stock, the stock runs through the top surface center setting of rotor plate, the top fixedly connected with runner of stock, the runner one end fixedly connected with latch of keeping away from of stock, the draw-in groove has been seted up at the bottom surface center of rotor plate, another draw-in groove has been seted up at the top surface center of plectane, the latch is the cross-section and is the hexagon setting, the draw-in groove sets up with the latch is matchd each other.
Preferably, first spout has all been seted up on the lateral wall that the diaphragm is close to through-hole center one side, the center fixedly connected with pivot of first spout, swing joint has the third gear on the lateral wall of pivot, the first slider of equal fixedly connected with on the both sides lateral wall of arc, swing joint sets up between first slider and the first spout, equal fixedly connected with rack, both sides on the lateral wall that first slider is close to third gear one side the rack sets up from top to bottom, both sides the rack all sets up with third gear engagement.
Preferably, a second sliding groove is formed in the side wall of the rotating plate, a second sliding block is fixedly connected to one end, far away from the arc-shaped plate, of the round rod, and the second sliding block and the second sliding groove are movably connected.
Preferably, the third spout has all been seted up on the lateral wall about the shell, equal fixedly connected with picture peg on the lateral wall about the fly leaf, picture peg and third spout swing joint set up, the picture peg is kept away from fly leaf one side and is extended the third spout and set up the screw hole that runs through its upper and lower lateral wall, threaded hole swing joint has the threaded rod, the equal swing joint in upper and lower both ends of threaded rod has the connecting plate, the connecting plate sets up with shell fixed connection, the top through connection board fixedly connected with round wheel of threaded rod.
preferably, the splint have all been seted up on being close to the lateral wall of push pedal one side and have erect the groove, the push pedal is the cross setting, the lateral wall of push pedal all inserts and erects the inslot and set up, the push pedal with erect swing joint between the groove and set up.
preferably, the side wall of the clamping plate close to one side of the bottom plate is fixedly connected with an anti-slip pad, and the side wall of the roller is fixedly connected with the anti-slip pad.
A method for using a separation device capable of realizing the preparation of a plurality of N-terminal brain natriuretic peptide precursor recombinant proteins comprises the following steps:
s1: firstly, placing a reagent bottle filled with an N-terminal brain natriuretic peptide precursor recombinant protein solvent into the through hole, enabling the bottom of the reagent bottle to be in contact with the bottom plate, and repeatedly placing a plurality of reagent bottles;
S2: then the long rod is pulled to enable the clamping teeth on the long rod to be combined with the clamping grooves on the lower side of the rotating plate, and then the rotating wheel is rotated to drive the rotating plate to rotate;
s3: the second sliding block moves in the second sliding groove through rotation of the rotating plate, the second sliding block drives the round rod to push the arc-shaped plate to move, the first sliding block on the arc-shaped plate drives the rack to be meshed with the third gear to rotate, the arc-shaped plates on two sides are close to each other, the reagent bottle is fixed through the roller, shaking of the reagent bottle is avoided, and the long rod is pressed after adjustment is completed, so that the clamping teeth are combined with the clamping grooves in the round plate;
S4: then the round wheel is rotated to drive the threaded rod to rotate, so that the threaded rod drives the inserting plate to move upwards in the third sliding groove, the movable plate is lifted, the bottom plate is kept still through the reagent bottles, the upper sides of the clamping plates are close to each other through the push plates on the vertical rods by the bottom plate, and the reagent bottles are clamped by the clamping plates;
S5: then starting a motor through an external power supply, driving a first gear on a main shaft to be meshed with a second gear to rotate by the motor, and driving a reagent bottle to rotate through a clamping device on the second gear to realize the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein;
S6: after the separation is finished, the power supply of the motor is turned off;
S7: the round wheel is rotated reversely, the round wheel drives the threaded rod to rotate reversely, so that the inserting plate descends in the third sliding groove, and the clamping device loosens the reagent bottle;
s8: then the long rod is pulled, so that the clamping teeth on the long rod are combined with the clamping grooves on the lower side of the rotating plate, and then the rotating wheel is rotated reversely to drive the rotating plate to rotate reversely, so that the loosening operation of the reagent bottle is realized;
S9: and taking out the reagent bottle to finish the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein.
compared with the prior art, the invention has the beneficial effects that: according to the reagent bottle fixing device, the clamping device is arranged, the threaded rod is rotated to drive the inserting plate to move in the third sliding groove, so that the bottom plate is in contact with the reagent bottle, the push plate is pushed to drive the clamping plate to move, the reagent bottle is clamped by the clamping plate, the reagent bottle fixing device can adapt to the fixing operation of reagent bottles with different diameters, and the universality of the reagent bottle fixing device is greatly improved; the rotating plate is arranged, the rotating device drives the rotating plate to rotate, so that the round rod drives the arc-shaped plate to move, the roller wheels clamp the reagent bottles, the stability of the reagent bottles in the centrifugal protein preparation process is effectively improved, the centrifugal operation of the reagent bottles can be realized by arranging the through holes and the clamping devices, and the working efficiency of the device is greatly improved; through being provided with the slipmat, the effectual damage to the reagent bottle of having avoided.
drawings
FIG. 1 is a schematic view of the internal structure of the present invention;
FIG. 2 is a schematic top view of the present invention;
FIG. 3 is an enlarged view taken at A of FIG. 1 in accordance with the present invention;
FIG. 4 is an enlarged view of FIG. 1 at B according to the present invention;
FIG. 5 is a schematic perspective view of a push plate according to the present invention;
fig. 6 is a schematic structural view of the latch of the present invention.
reference numbers in the figures: 1. a housing; 2. a circular plate; 3. a transverse plate; 4. an arc-shaped plate; 5. a roller; 6. a round bar; 7. a flat plate; 8. a vertical plate; 9. a first spring; 10. a rotating plate; 11. a rotating device; 12. a movable plate; 13. a motor; 14. a first gear; 15. a second gear; 16. a clamping device; 17. a rectangular groove; 18. a splint; 19. a circular shaft; 20. pushing the plate; 21. a second spring; 22. a vertical rod; 23. a base plate; 24. a long rod; 25. a rotating wheel; 26. clamping teeth; 27. a card slot; 28. a first chute; 29. a rotating shaft; 30. a third gear; 31. a first slider; 32. a rack; 33. a second chute; 34. a second slider; 35. a third chute; 36. inserting plates; 37. a threaded rod; 38. a connecting plate; 39. a round wheel; 40. a vertical slot; 41. a non-slip mat; 42. a main shaft.
Detailed Description
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, the present invention provides a technical solution: a separation device capable of realizing preparation of a plurality of N-terminal brain natriuretic peptide precursor recombinant proteins comprises a shell 1, and is characterized in that: the shell 1 is arranged in a circular shape, a circular plate 2 is fixedly connected to the position, close to the top surface, of the inner cavity of the shell 1, a plurality of through holes are uniformly distributed on the circular plate 2, the through holes penetrate through the upper side wall and the lower side wall of the circular plate 2, a transverse plate 3 is fixedly connected to both sides, located at the through holes, of the top surface of the circular plate 2, two arc plates 4 are connected between the two transverse plates 3, a first sliding groove 28 is formed in the side wall, close to the center of each through hole, of each transverse plate 3, a rotating shaft 29 is fixedly connected to the center of each first sliding groove 28, a third gear 30 is movably connected to the side wall of each rotating shaft 29, first sliding blocks 31 are fixedly connected to the side walls on both sides of each arc plate 4, each first sliding block 31 is movably connected to each first sliding groove 28, racks 32 are fixedly connected to the side wall, realize the relative motion of the arc plates 4, realize the centering operation of the reagent bottle, facilitate the clamping operation in the later period, the arc surfaces of the arc plates 4 are arranged towards one side of the center of the through hole, two idler wheels 5 are symmetrically and movably connected on the arc surfaces of the two arc plates 4, a round bar 6 is fixedly connected at the center of the straight surface of the arc plate 4 close to one side of the center of the circular plate 2, a flat plate 7 is fixedly connected on the side wall of the round bar 6, a vertical plate 8 is movably connected on the side wall of the round bar 6 close to one side of the center of the circular plate 2, the vertical plate 8 is fixedly connected with the circular plate 2, a first spring 9 is sleeved on the round bar 6, one end of the first spring 9 is fixedly connected on the flat plate 7, the other end of the first spring 9 is fixedly connected on the vertical plate 8, a rotating plate 10 is arranged at the center of the top surface of the circular plate 2, one ends of the plurality of, the end of the round rod 6 far away from the arc-shaped plate 4 is fixedly connected with a second slider 34, the second slider 34 is movably connected with a second sliding groove 33, the center of the rotating plate 10 is provided with a rotating device 11, the rotating device 11 comprises a long rod 24, the long rod 24 penetrates through the center of the top surface of the rotating plate 10, the top end of the long rod 24 is fixedly connected with a rotating wheel 25, one end of the long rod 24 far away from the rotating wheel 25 is fixedly connected with a latch 26, the center of the bottom surface of the rotating plate 10 is provided with a clamping groove 27, the center of the top surface of the round plate 2 is provided with another clamping groove 27, the section of the latch 26 is arranged in a hexagon shape, the clamping groove 27 and the latch 26 are mutually matched, so as to realize the fixation of the reagent bottle, and ensure that the reagent bottle is more stable and efficient during separation, the inner cavity of the shell 1 is provided with, the left side wall and the right side wall of the movable plate 12 are both fixedly connected with inserting plates 36, the inserting plates 36 are movably connected with the third sliding grooves 35, one side of the inserting plates 36, which is far away from the movable plate 12, extends out of the third sliding grooves 35 to be provided with threaded holes penetrating through the upper side wall and the lower side wall of the inserting plates, threaded rods 37 are movably connected in the threaded holes, the upper ends and the lower ends of the threaded rods 37 are both movably connected with connecting plates 38, the connecting plates 38 are fixedly connected with the shell 1, the top ends of the threaded rods 37 penetrate through the connecting plates 38 to be fixedly connected with round wheels 39 to realize the clamping operation of reagent bottles, the center of the bottom surface of the movable plate 12 is fixedly connected with a motor 13, a main shaft 42 is fixedly connected on a rotor of the motor 13, one end, which is far away from the motor, a plurality of second gears 15 are arranged in a manner that first gears 14 are meshed with each other, a clamping device 16 is arranged on each second gear 15, each clamping device 16 comprises a rectangular groove 17, four rectangular grooves 17 are uniformly distributed on each second gear 15, each rectangular groove 17 penetrates through the upper side wall and the lower side wall of each second gear 15, a clamping plate 18 is arranged in each rectangular groove 17, each clamping plate 18 is positioned on the corresponding side wall of the corresponding rectangular groove 17 and is fixedly connected with a round shaft 19, the two ends of each round shaft 19 are movably connected with the corresponding side wall of each rectangular groove 17, a movable plate 12 is positioned on the lower side of each second gear 15 and is provided with a round groove, the bottom surface of each clamping plate 18 is inserted into each round groove, a push plate 20 is arranged between the four clamping plates 18, the push plates 20 and the clamping plates 18 are movably connected, vertical grooves 40 are formed in the side walls, close to one sides of the push plates 20, of, swing joint sets up between push pedal 20 and the vertical groove 40, fixedly connected with second spring 21 on the bottom surface of push pedal 20, second spring 21 other end fixed connection is on the bottom surface of circular slot, the top surface center fixedly connected with montant 22 of push pedal 20, montant 22 is kept away from push pedal 20 one end and is run through 15 fixedly connected with bottom plates 23 of second gear, swing joint sets up between montant 22 and the second gear 15, the realization is to the tight operation of clamp of reagent bottle, the convenient separation operation to the solution in the reagent bottle, and can fix the reagent bottle use of different diameters, the commonality is good, splint 18 is close to equal fixedly connected with slipmat 41 on the lateral wall of bottom plate 23 one side, fixedly connected with slipmat 41 on the lateral wall of gyro wheel 5, avoid the damage to the reagent bottle.
A method for using a separation device capable of realizing the preparation of a plurality of N-terminal brain natriuretic peptide precursor recombinant proteins comprises the following steps:
S1: firstly, placing a reagent bottle filled with an N-terminal brain natriuretic peptide precursor recombinant protein solvent into the through hole, enabling the bottom of the reagent bottle to be in contact with the bottom plate 23, and repeatedly placing a plurality of reagent bottles;
S2: then the long rod 24 is pulled to make the latch 26 on the long rod 24 combine with the slot 27 on the lower side of the rotating plate 10, and then the rotating wheel 25 is rotated to drive the rotating plate 10 to rotate;
s3: through the rotation of the rotating plate 10, the second sliding block 34 moves in the second sliding groove 33, the second sliding block 34 drives the round rod 6 to push the arc-shaped plate 4 to move, the first sliding block 31 on the arc-shaped plate 4 drives the rack 32 to be meshed with the third gear 30 to rotate, the arc-shaped plates 4 on two sides are close to each other, the reagent bottle is fixed through the roller 5, the reagent bottle is prevented from shaking, and the long rod 24 is pressed after the adjustment is completed, so that the latch 26 is combined with the clamping groove 27 on the round plate 2;
s4: then, the round wheel 39 is rotated to drive the threaded rod 37 to rotate, so that the threaded rod 37 drives the inserting plate 36 to move upwards in the third sliding groove 35, the movable plate 12 is lifted, the bottom plate 23 is kept still through the reagent bottles, the upper sides of the clamping plates 18 are close to each other through the pushing plate 20 on the vertical rods 22 of the bottom plate 23, and the reagent bottles are clamped by the clamping plates 18;
s5: then starting the motor 13 through an external power supply, driving the first gear 14 on the main shaft 42 to be meshed with the second gear 15 to rotate by the motor 13, and driving the reagent bottle to rotate through the clamping device 16 on the second gear 15 to realize the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein;
s6: when the separation is completed, the power supply of the motor 13 is turned off;
S7: the round wheel 39 is rotated reversely, the round wheel 39 drives the threaded rod 37 to rotate reversely, so that the inserting plate 36 descends in the third sliding groove 35, and the clamping device 16 loosens the reagent bottle;
S8: then the long rod 24 is pulled, so that the latch 26 on the long rod 24 is combined with the clamping groove 27 on the lower side of the rotating plate 10, and then the rotating wheel 25 is rotated reversely to drive the rotating plate 10 to rotate reversely, so that the loosening operation of the reagent bottle is realized;
s9: and taking out the reagent bottle to finish the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein.
The working principle is as follows: when the invention is used, firstly, a reagent bottle filled with an N-terminal brain natriuretic peptide precursor recombinant protein solvent is placed in the through hole, so that the bottom of the reagent bottle is contacted with the bottom plate 23, a plurality of reagent bottles are repeatedly placed, the operation of the plurality of reagent bottles can be realized, the working efficiency is high, then the long rod 24 is pulled, the latch 26 on the long rod 24 is combined with the clamping groove 27 on the lower side of the rotating plate 10, then the rotating wheel 25 is rotated to drive the rotating plate 10 to rotate, the second slide block 34 is driven to move in the second sliding groove 33 through the rotation of the rotating plate 10, the second slide block 34 drives the round rod 6 to push the arc-shaped plate 4 to move, the first slide block 31 on the arc-shaped plate 4 drives the rack 32 to be meshed with the third gear 30 to rotate, the arc-shaped plates 4 on the two sides are close to each other, the reagent bottle is fixed through the roller 5, the shaking of the reagent bottle is avoided, the long rod 24 is pressed, then the round wheel 39 is rotated to drive the threaded rod 37 to rotate, so that the threaded rod 37 drives the inserting plate 36 to move upwards in the third sliding groove 35, the movable plate 12 is made to move upwards, the bottom plate 23 is kept still through the reagent bottle, the upper sides of the clamping plates 18 are close to each other through the push plate 20 on the vertical rod 22, the clamping plates 18 clamp the reagent bottle, then the motor 13 is started through an external power supply, the motor 13 drives the first gear 14 on the main shaft 42 to be meshed with the second gear 15 to rotate, the clamping device 16 on the second gear 15 drives the reagent bottle to rotate, the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein is realized, after the separation is finished, the power supply of the motor 13 is closed, the round wheel 39 is rotated reversely, the round wheel 39 drives the threaded rod 37 to rotate reversely, so that the inserting plate 36 moves downwards in the third sliding groove 35, the clamping device 16 loosens the, the clamping teeth 26 on the long rod 24 are combined with the clamping grooves 27 on the lower side of the rotating plate 10, then the rotating wheel 25 is rotated reversely to drive the rotating plate 10 to rotate reversely, so that the loosening operation of the reagent bottle is realized, the reagent bottle is taken out, and the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein is completed.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. a separation device enabling the production of a plurality of N-telopeptide precursor recombinant proteins, comprising a housing (1), characterized in that: the shell (1) is in a circular arrangement, a circular plate (2) is fixedly connected to the position, close to the top surface, of an inner cavity of the shell (1), a plurality of through holes are formed in the circular plate (2) in an evenly distributed mode, the through holes penetrate through the upper side wall and the lower side wall of the circular plate (2), transverse plates (3) are fixedly connected to the two sides, located at the through holes, of the top surface of the circular plate (2), two arc-shaped plates (4) are connected between the two transverse plates (3), arc-shaped surfaces of the arc-shaped plates (4) are arranged towards one side of the center of the through holes, two idler wheels (5) are symmetrically and movably connected to the arc-shaped surfaces of the two arc-shaped plates (4), a circular rod (6) is fixedly connected to the straight surface center of the arc-shaped plate (4) close to one side of the center of the circular plate (2), a flat plate (7) is fixedly connected, the circular plate structure is characterized in that a vertical plate (8) is fixedly connected with a circular plate (2), a first spring (9) is sleeved on a circular rod (6), one end of the first spring (9) is fixedly connected onto a flat plate (7), the other end of the first spring (9) is fixedly connected onto the vertical plate (8), a rotating plate (10) is arranged at the center of the top surface of the circular plate (2), a plurality of circular rods (6) are far away from one end of an arc plate (4) and are movably connected with the rotating plate (10), a rotating device (11) is arranged at the center of the rotating plate (10), a movable plate (12) is arranged at the lower side of the circular plate (2) of an inner cavity of a shell (1), the movable plate (12) is movably connected with the shell (1), a motor (13) is fixedly connected with the center of the bottom surface of the movable plate (12), and a main shaft (42) is fixedly connected onto, motor (13) one end is kept away from in main shaft (42) runs through first gear (14) of fly leaf (12) fixedly connected with, swing joint sets up between main shaft (42) and fly leaf (12), evenly distributed swing joint has a plurality of second gear (15), a plurality of on the top surface of fly leaf (12) even first gear (14) intermeshing setting of second gear (15), be provided with clamping device (16) on second gear (15).
2. The separation device of claim 1, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: the clamping device (16) comprises rectangular grooves (17), four rectangular grooves (17) are uniformly distributed on the second gear (15), the rectangular grooves (17) penetrate through the upper side wall and the lower side wall of the second gear (15), clamping plates (18) are arranged in the rectangular grooves (17), the clamping plates (18) are positioned on the side walls of the inner cavity of the rectangular grooves (17) and fixedly connected with circular shafts (19), the side walls of the two ends of the circular shafts (19) are movably connected with the rectangular grooves (17), a circular groove is formed in the lower side of the movable plate (12) positioned on the second gear (15), the bottom surfaces of the clamping plates (18) are inserted into the circular groove, push plates (20) are arranged between the four clamping plates (18), the push plates (20) are movably connected with the clamping plates (18), second springs (21) are fixedly connected to the bottom surfaces of the push plates (20), and the other ends of the second springs (21) are fixedly connected to the bottom surfaces of the circular grooves, the top surface center fixedly connected with montant (22) of push pedal (20), montant (22) are kept away from push pedal (20) one end and are run through second gear (15) fixedly connected with bottom plate (23), swing joint sets up between montant (22) and second gear (15).
3. The separation device of claim 1, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: rotating device (11) are including stock (24), the top surface center that stock (24) run through rotor plate (10) sets up, top fixedly connected with runner (25) of stock (24), keeping away from runner (25) one end fixedly connected with latch (26) of stock (24), draw-in groove (27) have been seted up at the bottom surface center of rotor plate (10), another draw-in groove (27) have been seted up at the top surface center of plectane (2), latch (26) are the cross-section and are the hexagon setting, draw-in groove (27) and latch (26) match the setting each other.
4. the separation device of claim 1, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: first spout (28) have all been seted up on diaphragm (3) the lateral wall that is close to through-hole center one side, the center fixedly connected with pivot (29) of first spout (28), swing joint has third gear (30) on the lateral wall of pivot (29), equal first slider (31) of fixedly connected with on the both sides lateral wall of arc (4), swing joint sets up between first slider (31) and first spout (28), equal fixedly connected with rack (32), both sides on first slider (31) the lateral wall that is close to third gear (30) one side rack (32) set up from top to bottom, both sides rack (32) all set up with third gear (30) meshing.
5. the separation device of claim 1, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: a second sliding groove (33) is formed in the side wall of the rotating plate (10), a second sliding block (34) is fixedly connected to one end, far away from the arc-shaped plate (4), of the round rod (6), and the second sliding block (34) is movably connected with the second sliding groove (33).
6. The separation device of claim 1, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: third spout (35) have all been seted up on the lateral wall about shell (1), equal fixedly connected with picture peg (36) on the lateral wall about fly leaf (12), picture peg (36) set up with third spout (35) swing joint, picture peg (36) are kept away from fly leaf (12) one side and are extended third spout (35) and set up the screw hole that runs through its upper and lower lateral wall, threaded hole swing joint has threaded rod (37), the equal swing joint in upper and lower both ends of threaded rod (37) has connecting plate (38), connecting plate (38) set up with shell (1) fixed connection, the top of threaded rod (37) runs through connecting plate (38) fixedly connected with round wheel (39).
7. The separation device of claim 2, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: all seted up on splint (18) are close to the lateral wall of push pedal (20) one side and erected groove (40), push pedal (20) are the cross setting, the lateral wall of push pedal (20) all inserts and erects the inslot (40) and set up, swing joint sets up between push pedal (20) and the perpendicular groove (40).
8. The separation device of claim 2, wherein the separation device is capable of producing a plurality of N-terminal natriuretic peptide precursor recombinant proteins, and the separation device comprises: the side wall of the clamping plate (18) close to one side of the bottom plate (23) is fixedly connected with an anti-slip pad (41), and the side wall of the roller (5) is fixedly connected with the anti-slip pad (41).
9. The method of using the device according to any one of claims 1-8 for preparing a plurality of recombinant proteins of the precursor N-telopeptide, comprising the steps of:
S1: firstly, placing a reagent bottle filled with an N-terminal brain natriuretic peptide precursor recombinant protein solvent into the through hole, enabling the bottom of the reagent bottle to be in contact with a bottom plate (23), and repeatedly placing a plurality of reagent bottles;
s2: then the long rod (24) is pulled to enable the clamping teeth (26) on the long rod (24) to be combined with the clamping grooves (27) on the lower side of the rotating plate (10), and then the rotating wheel (25) is rotated to drive the rotating plate (10) to rotate;
S3: through the rotation of the rotating plate (10), the second sliding block (34) moves in the second sliding groove (33), the second sliding block (34) drives the round rod (6) to push the arc-shaped plate (4) to move, the first sliding block (31) on the arc-shaped plate (4) drives the rack (32) to be meshed with the third gear (30) to rotate, the arc-shaped plates (4) on the two sides are close to each other, the reagent bottle is fixed through the roller (5), the reagent bottle is prevented from shaking, and the long rod (24) is pressed after the adjustment is completed, so that the latch (26) is combined with the clamping groove (27) on the round plate (2);
S4: then, the round wheel (39) is rotated to drive the threaded rod (37) to rotate, so that the threaded rod (37) drives the inserting plate (36) to move upwards in the third sliding groove (35), the movable plate (12) is made to ascend, the bottom plate (23) is kept to be stationary through the reagent bottles, the upper sides of the clamping plates (18) are close to each other through the vertical rods (22) and the push plates (20) on the bottom plate (23), and the reagent bottles are clamped by the clamping plates (18);
S5: then, a motor (13) is started through an external power supply, the motor (13) drives a first gear (14) on a main shaft (42) to be meshed with a second gear (15) to rotate, and a clamping device (16) on the second gear (15) drives a reagent bottle to rotate, so that the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein is realized;
S6: when the separation is finished, the power supply of the motor (13) is turned off;
S7: the round wheel (39) is rotated reversely, the round wheel (39) drives the threaded rod (37) to rotate reversely, so that the inserting plate (36) descends in the third sliding groove (35) to enable the clamping device (16) to loosen the reagent bottle;
s8: then the long rod (24) is pulled to enable the clamping teeth (26) on the long rod (24) to be combined with the clamping grooves (27) on the lower side of the rotating plate (10), and then the rotating wheel (25) is rotated reversely to drive the rotating plate (10) to rotate reversely, so that the loosening operation of the reagent bottles is realized;
s9: and taking out the reagent bottle to finish the separation operation of the N-terminal brain natriuretic peptide precursor recombinant protein.
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Denomination of invention: The invention relates to a separation device capable of preparing a plurality of N-terminal brain natriuretic peptide precursor (NT proBNP) recombinant proteins and an application method thereof Effective date of registration: 20210831 Granted publication date: 20210601 Pledgee: Nanjing Bank Co.,Ltd. Nanjing Financial City Branch Pledgor: Nanjing Shenji Pharmaceutical Technology Co.,Ltd. Registration number: Y2021980008618 |
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Date of cancellation: 20230106 Granted publication date: 20210601 Pledgee: Nanjing Bank Co.,Ltd. Nanjing Financial City Branch Pledgor: Nanjing Shenji Pharmaceutical Technology Co.,Ltd. Registration number: Y2021980008618 |