CN207608547U - Cell culture culture bottle fixed frame - Google Patents
Cell culture culture bottle fixed frame Download PDFInfo
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- CN207608547U CN207608547U CN201721710843.6U CN201721710843U CN207608547U CN 207608547 U CN207608547 U CN 207608547U CN 201721710843 U CN201721710843 U CN 201721710843U CN 207608547 U CN207608547 U CN 207608547U
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- supporting plate
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- 238000004113 cell culture Methods 0.000 title claims abstract description 10
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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Abstract
本实用新型公开了一种细胞培养用培养瓶固定架,属于细胞培养用具技术领域。底板底部四周设有吸盘,底板顶部固定有一对立板,两立板内均设有轴承,两轴承内设有一根转轴,两立板间的转轴上设有一固定在其侧壁的托板,托板前端面左侧设有挡板,托板上开有一对滑槽,滑槽内设有拉簧,两拉簧一端固定在挡板上,另一端与夹板连接;底板顶部设有一控制箱,控制箱内设有单片机,单片机上设有与外界电源连接的电源线,控制箱前方设有固定在底板上的托板垂直按钮、托板水平按钮和托板45°倾斜按钮,控制箱顶部设有电机,电机轴端连接有减速机,减速机轴端设有小齿轮;其中,所述的立板外侧的转轴上设有一大齿轮,小齿轮与大齿轮齿啮。
The utility model discloses a culture bottle fixing frame for cell culture, which belongs to the technical field of cell culture equipment. There are suction cups around the bottom of the bottom plate, a pair of vertical plates are fixed on the top of the bottom plate, bearings are arranged in the two vertical plates, a rotating shaft is arranged in the two bearings, and a supporting plate fixed on the side wall of the two vertical plates is arranged on the rotating shaft between the two vertical plates. There is a baffle on the left side of the front end of the plate, a pair of chute is opened on the supporting plate, and a tension spring is arranged in the chute, one end of the two tension springs is fixed on the baffle, and the other end is connected with the splint; there is a control box on the top of the bottom plate, There is a single-chip microcomputer in the control box, and the power line connected to the external power supply is set on the single-chip microcomputer. The vertical button of the pallet, the horizontal button of the pallet and the 45° tilt button of the pallet are fixed on the front of the control box. The top of the control box is set There is a motor, the shaft end of the motor is connected with a reducer, and the shaft end of the reducer is provided with a pinion; wherein, a large gear is provided on the rotating shaft outside the vertical plate, and the small gear meshes with the large gear.
Description
技术领域technical field
本实用新型涉及一种细胞培养用培养瓶固定架,属于细胞培养用具技术领域。The utility model relates to a culture bottle fixing frame for cell culture, which belongs to the technical field of cell culture equipment.
背景技术Background technique
细胞原代培养的接种过程中,细胞数或组织块一定要合适,保证在24小时内有充足的营养,24小时换一次液,一定要注意轻拿轻放,最好先将培养瓶慢慢立起,再拿出,换液后慢慢放平。24小时换液后,最好在6~7天之内不要动培养瓶,给细胞一个贴壁适应的时间。等生长成片以后,才可以消化传代。整个过程看似简单,但是很多操作者往往做了1-2个月,都因为不贴壁,导致无法完成。整个操作过程中,无论哪次稍微大力晃动了培养瓶,都会是导致整个实验失败的主要原因。During the inoculation process of primary cell culture, the number of cells or tissue pieces must be appropriate to ensure sufficient nutrition within 24 hours, and the liquid should be changed every 24 hours. Be careful to handle it carefully. Stand it up, take it out again, and slowly lay it flat after changing the liquid. After changing the medium for 24 hours, it is best not to move the culture flask within 6-7 days to give the cells a time to adapt to the wall. After growing into pieces, they can be digested and passaged. The whole process seems simple, but many operators often do it for 1-2 months, but they cannot complete it because they are not attached to the wall. During the entire operation, no matter which time the flask was shaken vigorously, it would be the main reason for the failure of the entire experiment.
实用新型内容Utility model content
为了解决上述现有技术存在的不足,本实用新型提供了一种细胞培养用培养瓶固定架,确保培养瓶缓慢立起或放倒,保证实验过程的稳定性,提高实验的成功率。In order to solve the deficiencies in the above-mentioned prior art, the utility model provides a culture bottle fixing frame for cell culture, which ensures that the culture bottle is slowly erected or laid down, ensures the stability of the experiment process, and improves the success rate of the experiment.
本实用新型解决其技术问题所采用的技术方案是:一种细胞培养用培养瓶固定架,包括底板、立板、轴承、大齿轮、转轴、挡板、托板、拉簧、夹板、小齿轮、减速机、电机、控制箱、托板垂直按钮、托板水平按钮、托板45°倾斜按钮和吸盘,底板为一矩形板体,底板底部四周设有吸盘,底板顶部垂直固定有一对面面平行的立板,两立板内均设有轴承,两轴承内设有一根转轴,两立板间的转轴上设有一固定在其侧壁的托板,托板前端面左侧设有挡板,托板上开有一对滑槽,滑槽内设有拉簧,两拉簧一端固定在挡板上,另一端与夹板连接;底板顶部立板左侧设有一控制箱,控制箱内设有单片机,单片机上设有与外界电源连接的电源线,控制箱前方设有固定在底板上的托板垂直按钮、托板水平按钮和托板45°倾斜按钮,控制箱顶部设有电机,电机轴端连接有减速机,减速机轴端设有小齿轮;其中,所述的立板外侧的转轴上设有一大齿轮,小齿轮与大齿轮齿啮;所述的控制箱内的单片机通过导线与电机、托板垂直按钮、托板水平按钮和托板45°倾斜按钮分别连接。The technical solution adopted by the utility model to solve the technical problem is: a culture bottle fixing frame for cell culture, including a bottom plate, a vertical plate, a bearing, a large gear, a rotating shaft, a baffle plate, a supporting plate, an extension spring, a splint, and a pinion , reducer, motor, control box, pallet vertical button, pallet horizontal button, pallet 45° inclined button and suction cup. There are bearings in the two vertical plates, a rotating shaft is arranged in the two bearings, a supporting plate fixed on the side wall is arranged on the rotating shaft between the two vertical plates, and a baffle plate is provided on the left side of the front end of the supporting plate. There is a pair of chute on the supporting plate, there is a tension spring in the chute, one end of the two tension springs is fixed on the baffle, and the other end is connected to the splint; there is a control box on the left side of the vertical plate on the top of the bottom plate, and a single chip microcomputer is installed in the control box , the single chip microcomputer is equipped with a power line connected to the external power supply, the front of the control box is equipped with a vertical button for the pallet, a horizontal button for the pallet and a 45° tilt button for the pallet, and a motor is installed on the top of the control box. A reducer is connected, and the shaft end of the reducer is provided with a pinion; wherein, a large gear is provided on the rotating shaft outside the vertical plate, and the pinion and the large gear are meshed; the single-chip microcomputer in the control box is connected with the motor , the pallet vertical button, the pallet horizontal button and the pallet 45° inclined button are connected respectively.
本实用新型的有益效果是:避免了细胞原代培养的接种过程中,因培养瓶换液过程中操作不稳定而导致实验失败的因素,整个实验均可在该架体上操作,确保培养瓶缓慢立起或放倒,预防换液过程中培养瓶的晃动;防止实验过程中移动培养瓶而导致晃动的问题。The beneficial effects of the utility model are: in the inoculation process of the primary culture of cells, the factor of experiment failure due to unstable operation in the process of changing the liquid of the culture bottle is avoided, and the whole experiment can be operated on the frame body, ensuring that the culture bottle Slowly stand up or lay down to prevent the shake of the culture bottle during the liquid change process; prevent the shaking caused by moving the culture bottle during the experiment.
附图说明Description of drawings
下面结合附图和具体实施方式对本实用新型进一步说明。Below in conjunction with accompanying drawing and specific embodiment the utility model is further described.
图1为本实用新型局部剖视后的主视图。Fig. 1 is the front view of the utility model after partial section.
图2为本实用新型的侧视图。Fig. 2 is a side view of the utility model.
图3为本实用新型的A-A剖视图。Fig. 3 is A-A sectional view of the utility model.
图中标号:Labels in the figure:
1、底板,2、立板,3、轴承,4、大齿轮,5、转轴,6、挡板,7、托板,8、拉簧,9、滑槽,10、夹板,11、小齿轮,12、减速机,13、电机,14、控制箱,15、托板垂直按钮,16、托板水平按钮,17、托板45°倾斜按钮,18、吸盘。1. Bottom plate, 2. Vertical plate, 3. Bearing, 4. Big gear, 5. Rotating shaft, 6. Baffle plate, 7. Support plate, 8. Extension spring, 9. Chute, 10. Splint plate, 11. Pinion gear , 12, reducer, 13, motor, 14, control box, 15, supporting plate vertical button, 16, supporting plate horizontal button, 17, supporting plate 45 ° inclined button, 18, sucker.
具体实施方式Detailed ways
如图1-3所示,一种细胞培养用培养瓶固定架,包括底板1、立板2、轴承3、大齿轮4、转轴5、挡板6、托板7、拉簧8、夹板10、小齿轮11、减速机12、电机13、控制箱14、托板垂直按钮15、托板水平按钮16、托板45°倾斜按钮17和吸盘18,底板1为一矩形板体,底板1底部四周设有吸盘18,底板1顶部垂直固定有一对面面平行的立板2,两立板2内均设有轴承3,两轴承3内设有一根转轴5,两立板2间的转轴5上设有一固定在其侧壁的托板7,托板7前端面左侧设有挡板6,托板7上开有一对滑槽9,滑槽9内设有拉簧8,两拉簧8一端固定在挡板5上,另一端与夹板10连接;底板1顶部立板2左侧设有一控制箱14,控制箱14内设有单片机,单片机上设有与外界电源连接的电源线,控制箱14前方设有固定在底板1上的托板垂直按钮15、托板水平按钮16和托板45°倾斜按钮17,控制箱14顶部设有电机13,电机13轴端连接有减速机12,减速机12轴端设有小齿轮11;其中,所述的立板2外侧的转轴5上设有一大齿轮4,小齿轮11与大齿轮4齿啮;所述的控制箱14内的单片机通过导线与电机13、托板垂直按钮15、托板水平按钮16和托板45°倾斜按钮17分别连接。As shown in Figures 1-3, a culture bottle fixing frame for cell culture includes a bottom plate 1, a vertical plate 2, a bearing 3, a large gear 4, a rotating shaft 5, a baffle plate 6, a supporting plate 7, a tension spring 8, and a splint 10 , pinion 11, reducer 12, motor 13, control box 14, pallet vertical button 15, pallet horizontal button 16, pallet 45° inclined button 17 and suction cup 18, base plate 1 is a rectangular plate body, base plate 1 bottom Suction cups 18 are arranged around, and a pair of parallel vertical plates 2 are vertically fixed on the top of the bottom plate 1. Bearings 3 are arranged in the two vertical plates 2, and a rotating shaft 5 is arranged in the two bearings 3. On the rotating shaft 5 between the two vertical plates 2 There is a supporting plate 7 fixed on its side wall, a baffle plate 6 is arranged on the left side of the front end of the supporting plate 7, a pair of chute 9 is opened on the supporting plate 7, an extension spring 8 is arranged in the chute 9, and two extension springs 8 One end is fixed on the baffle plate 5, and the other end is connected with the clamping plate 10; a control box 14 is provided on the left side of the top vertical plate 2 of the base plate 1, and a single-chip microcomputer is arranged in the control box 14, and the power supply line connected with the external power supply is provided on the single-chip microcomputer to control The front of the box 14 is provided with a pallet vertical button 15, a pallet horizontal button 16, and a pallet 45° tilt button 17 fixed on the base plate 1. The top of the control box 14 is provided with a motor 13, and the shaft end of the motor 13 is connected with a reducer 12. The shaft end of reducer 12 is provided with pinion 11; Wherein, the rotating shaft 5 on the outside of described vertical plate 2 is provided with a large gear 4, and pinion 11 meshes with large gear 4; The single-chip microcomputer in the described control box 14 passes Lead is connected with motor 13, supporting plate vertical button 15, supporting plate horizontal button 16 and supporting plate 45 ° of inclination buttons 17 respectively.
使用时,将该工具通过吸盘18固定在平面上,将该工具通过导线连接电源,按动托板水平按钮16,使托板7水平朝上,将培养瓶放置于托板7上,通过挡板6和夹板10被固定,然后在该工具上进行细胞原代培养试验,需要培养瓶立起使,只需按动托板垂直按钮15,单片机控制电机13转动,通过小齿轮11与大齿轮4的齿啮,使转轴5上的托板7转动,便可使培养瓶缓慢翻转至竖立状态,同样,如果需要将培养瓶缓慢放倒时,即可按动托板水平按钮16;如果需要将培养瓶倾斜时,即可按动托板45°倾斜按钮17。During use, this tool is fixed on the plane by the sucker 18, this tool is connected to the power supply through the wire, press the supporting plate horizontal button 16, the supporting plate 7 is horizontally upward, the culture bottle is placed on the supporting plate 7, through the baffle The plate 6 and the splint 10 are fixed, and then the cell primary culture test is carried out on the tool. The culture bottle needs to be erected. Just press the vertical button 15 of the supporting plate, and the single-chip microcomputer controls the rotation of the motor 13. Through the small gear 11 and the large gear 4, the supporting plate 7 on the rotating shaft 5 is rotated, and the culture bottle can be slowly turned over to an upright state. Similarly, if the culture bottle needs to be slowly brought down, the supporting plate horizontal button 16 can be pressed; When the culture bottle is tilted, the 45° tilt button 17 of the supporting plate can be pressed.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721710843.6U CN207608547U (en) | 2017-12-11 | 2017-12-11 | Cell culture culture bottle fixed frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721710843.6U CN207608547U (en) | 2017-12-11 | 2017-12-11 | Cell culture culture bottle fixed frame |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207608547U true CN207608547U (en) | 2018-07-13 |
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ID=62797842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721710843.6U Expired - Fee Related CN207608547U (en) | 2017-12-11 | 2017-12-11 | Cell culture culture bottle fixed frame |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207608547U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113337400A (en) * | 2021-05-31 | 2021-09-03 | 北京艾颐生物科技有限公司 | Stem cell culture bottle support device and using method |
-
2017
- 2017-12-11 CN CN201721710843.6U patent/CN207608547U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113337400A (en) * | 2021-05-31 | 2021-09-03 | 北京艾颐生物科技有限公司 | Stem cell culture bottle support device and using method |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180713 Termination date: 20181211 |
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| CF01 | Termination of patent right due to non-payment of annual fee |