CN110563137A - Device and method for determining influence rule of pollution factors on biological activity of anaerobic process - Google Patents

Abstract

A device and method for measuring the law of influence of pollution factors on the biological activity of anaerobic process, which relates to a device and method for measuring the law of influence of anaerobic process biological activity. It aims to provide a device and method for measuring the influence of pollution factors on the biological activity of anaerobic process. The device of the present invention comprises multiple sets of reaction devices arranged in parallel, oxygen removal device, gas collection device and oscillation device; wherein each set of reaction device comprises a reaction vessel and a sealing cover; an air inlet and an air outlet are arranged on the sealing cover; The outlet is connected with the oxygen removal device, and the gas outlet is connected with the gas collection device; the reaction vessel is fixed on the oscillation device. Method: Add pollutant solution to multiple sets of reaction devices at the same time, operate in a cycle, test indicators during the period, calculate the removal rate of COD, ammonia nitrogen, nitrous nitrogen, and nitrate nitrogen and/or the change of removal load over time, and obtain the factors Effects on biological activity of wastewater anaerobic process. It can be used in the technical field of sewage treatment.

Description

A device for measuring the influence of pollution factors on the biological activity of anaerobic process and its method

technical field

The invention belongs to the technical field of sewage treatment, and in particular relates to a device and a method for measuring the law of influence of various pollution factors on the biological activity of anaerobic process.

Background technique

With the continuous development of sewage treatment technology, people gradually realize that sewage is no longer a simple waste treatment object, and the view that sewage is a carrier of resource and energy recovery is gradually becoming a consensus in the industry. Looking back at the development of sewage treatment technology, it is not difficult to find that the activated sludge method has been the protagonist in the field of water treatment since it was proposed. However, under the background of energy crisis and resource shortage, the concepts of sustainable development and carbon neutrality are increasingly mentioned, and the traditional aerobic process represented by the activated sludge method seems to be weak. Therefore, people There is an urgent need for some new technologies and concepts to lead this major turning point in the water treatment industry.

Anaerobic process refers to an efficient sewage treatment method in which organic matter in sewage is decomposed, metabolized, and digested by anaerobic bacteria under anaerobic conditions, so that the content of organic matter in sewage is greatly reduced and biogas is generated at the same time. Compared with the aerobic process, the anaerobic process does not require external energy, does not require aeration, saves energy consumption, and can also produce biogas such as methane, which has high economic benefits. In addition to the traditional anaerobic nitrification, the new autotrophic denitrification process represented by anammox is also an important part of the anaerobic process. The so-called anammox refers to the process in which anammox bacteria use ammonia nitrogen as an electron donor and nitrous as an electron acceptor to oxidize and reduce it to nitrogen under anaerobic conditions. Compared with traditional nitrification and denitrification, no additional carbon source is needed at all, the denitrification steps are shortened, and the amount of biological acid production and sludge production are greatly reduced. The proposal of anaerobic ammonium oxidation has pushed the anaerobic process to a new level. If this new type of anaerobic technology is combined with traditional anaerobic digestion technology, it will not only achieve low-cost collaborative removal of pollutants, but also reach the goal of sewage treatment plants. Energy is completely self-sufficient or even surplus, achieving the goal of carbon neutrality.

Microbes are the basis of sewage biological treatment, and their population, quantity and activity will directly affect the treatment effect. In addition, anaerobic microorganisms grow slowly and are sensitive to external conditions such as pH, dissolved oxygen, and temperature. In addition, the toxic substances in the water body may also have a certain impact on the anaerobic system, such as heavy metals, nanoparticles and antibiotics, etc. However, there is no special measurement device and method to test the influence of pollution factors on microorganisms. Therefore, it is of great significance for the further application of anaerobic technology to develop a set of fast and stable measuring devices and methods to ascertain the influence of these factors on microorganisms.

Contents of the invention

The object of the present invention is to provide a device and method for measuring the law of influence of pollution factors on the biological activity of anaerobic process.

A device for measuring the influence of pollution factors on the biological activity of anaerobic process of the present invention, comprising a plurality of sets of reaction devices 1, oxygen removal devices 2, gas collection devices 3 and oscillation devices 4 arranged in parallel;

The structure of multiple sets of reaction devices 1 arranged in parallel is the same; each set of reaction devices 1 includes a reaction volume 1-1 and a sealing cover 1-2; an air inlet 1-3 and an air outlet 1-4 are arranged on the sealing cover;

Deoxygenation device 2 comprises nitrogen cylinder 2-1, nitrogen cabinet 2-2 and a plurality of rotameters 2-3; Nitrogen cylinder 2-1 is connected with the air inlet of nitrogen cabinet 2-2; Nitrogen cabinet 2-2 When the gas outlet of the gas outlet and the rotameter 2-3 are connected with the gas inlet 1-3 of the reaction device 1, it is used for blowing off;

The gas collection device 3 includes a gas flowmeter 3-1 and a gas collection bag 3-2; when the gas outlet 1-4 is connected to the gas collection bag 3-2 through the gas flowmeter 3-1, it is used to collect the gas generated by the reaction;

The reaction vessels 1 - 1 of multiple sets of reaction devices 1 arranged in parallel are all fixed on the oscillation device 4 .

Further, the reaction device 1 also includes a sealing gasket 1-5 and a clamp 1-6, the sealing gasket 1-5 is arranged between the reaction vessel 1-1 and the sealing cover 1-2, and the clamp 1-6 is used to seal the The cover 1-2 is clamped with the reaction vessel 1-1;

Further, the volume of the reaction vessel 1-1 is 500-1000mL;

Further, the air inlet 1-3 is located at the center of the sealing cap and extends into the bottom of the digestion bottle;

Further, pneumatic one-way valves are provided at the air outlet 1-4 and the air inlet 1-3 to avoid gas backflow;

Further, a porous gas splitter is set between the nitrogen cabinet 2-2 and the plurality of rotameters 2-3 to make the nitrogen flow uniform;

Further, there are 3 to 10 sets of reaction devices 1 arranged in parallel, and the number of sets can be adjusted according to actual needs;

Further, the oscillating device 4 is a shaking table;

Utilize above-mentioned device measurement pollution factor to the method for anaerobic process biological activity influence rule, comprise the following steps:

1. Number multiple sets of reaction devices 1 arranged in parallel, respectively No. 0, No. 1, No. 2, ..., No. n, use water distribution to wash the sludge to be inoculated 2 to 3 times, and inoculate evenly into each reaction container In 1-1, the sludge concentration is controlled to be 5000-10000mg/L, and the volume and concentration of the mud-water mixture in each reaction vessel 1-1 are equal;

2. Take n equal parts of water, add various pollutants into it respectively, and mix evenly to obtain n kinds of solutions containing pollutants;

3. Change the water of No. 0 reaction vessel 1-1 with the original water according to the set water change ratio, and set it as a blank control; use the solution containing pollution factors according to the set water change ratio for the remaining corresponding reaction vessel 1 -1 Change the water, filter the mud-water mixture, record the sampling time and measure the pH and temperature at the same time, collect the filtrate to measure the corresponding initial pollution index, and return the sludge to the reactor; the initial pollution index refers to the concentration of organic matter [COD] _Inf. , ammonia nitrogen concentration [NH ₄ ⁺ -N] _Inf. , nitrous nitrogen concentration [NO ₂ ^- -N] _Inf. , nitrate nitrogen concentration [NO ₃ ^- -N] _Inf. and/or total nitrogen concentration [TN] _Inf .;

4. Cover the sealing cover 1-2 of the reaction vessel 1-1, connect the air inlet 1-3 with the oxygen removal device 2 with a trachea, open the oxygen removal device 2, and adjust each rotor flow control meter 2-3 to The flow rate of nitrogen is controlled at 0.5-1.5L/min, and the blow-off is performed for 5-10 minutes; after the blow-off is completed, first turn off the rotor flow control meters 2-3, then turn off the oxygen removal device 2, and pull out the trachea at the inlet pipe 1-3. Lose;

5. Connect the gas outlet 1-4 with the gas collecting device 3 with a gas pipe, and clear the gas flow meter 3-1;

6. Turn on the oscillation device 4, set the rotation speed of the oscillation device 4 to 100-300rpm, set the temperature to 25-35°C, and set the oscillation reaction time to 10-20h;

7. After the oscillation reaction is over, close the valve of the gas collection bag 3-2, record the corresponding data of the gas flow meter 3-1, remove the gas component to be measured from the gas collection bag 3-2, and connect the gas pipe at the gas outlet 1-4 unplug;

Eight, open the sealing cover 1-2 of the reaction vessel 1-1, take the mud-water mixture to filter and measure the pH and temperature, collect the filtrate to measure the corresponding end pollution index, and the sludge is poured back into the reactor; after the settlement is completed, use the water distribution to react respectively The sludge in the container 1-1 is washed 1 to 2 times; the corresponding end pollution indicators refer to the concentration of organic matter [COD] _Eff. , the concentration of ammonia nitrogen [NH ₄ ⁺ -N] _Eff. , the concentration of nitrous [NO ₂ ^- -N] _Eff. , nitrate nitrogen concentration [NO ₃ ^- -N] _Eff. and/or total nitrogen concentration [TN] _Eff .;

9. Repeat steps 2 to 8 to cycle, 1 to 2 cycles per day, and cycle for at least 30 days. After the cycle is over, the COD removal rate, COD removal load, ammonia nitrogen removal rate, nitrous nitrogen removal rate, Nitrate removal rate, total nitrogen removal rate and/or total nitrogen removal load are calculated:

In the formula, t is the reaction time (h);

Obtain the COD removal rate, COD removal load, ammonia nitrogen removal rate, nitrous nitrogen removal rate, nitrate nitrogen removal rate, total nitrogen removal rate and/or total nitrogen removal load corresponding to various pollution factors. The influence of various pollution factors on the biological activity of anaerobic process was obtained by pH and temperature changes.

Further, the water distribution in step 2 is based on the simulated configuration of the pollutant types and concentrations in the actual wastewater at the inoculated sludge sampling point. The configuration method is as follows: the concentration of COD is 0-5000 mg/L, and the concentration of ammonia nitrogen is 0-600 mg/L , the concentration of nitrous nitrogen is 0-300mg/L, the concentration of nitrate nitrogen is 0-300mg/L, the alkalinity is 0-2000mg/L, the concentration of total phosphorus is 0-20mg/L, and the C ₆ concentration of 0-4686.04mg/L H ₁₂ O ₆ , (NH ₄ ) ₂ SO ₄ from 0 to 2828.57 mg/L, NaNO ₂ from 0 to 1478.57 mg/L, NaNO ₃ from 0 to 1821.43 mg/L, NaHCO ₃ from 0 to 3356.64 mg/L and Add 0-87.74mg/L KH ₂ PO ₄ into water to dissolve, and add inorganic salts and trace elements needed for microbial growth; the inorganic salts are 100-200mg/L MgSO ₄ 7H ₂ O and 50-100mg/L L of CaCl ₂ ; trace elements are 4~6mg/L FeSO ₄ , 0.3~0.5mg/L ZnSO ₄ ·7H ₂ O, 0.1~0.3mg/L CoCl ₂ ·6H ₂ O, 0.5~1.5mg/L L MnCl ₂ 4H ₂ O, 0.1~0.3mg/L CuSO ₄ 5H ₂ O, 0.1~0.3mg/L Na ₂ MoO ₄ 2H ₂ O, 0.1~0.3mg/L NiCl ₂ 6H ₂ O and 0.1-0.3 mg/L Na ₂ SeO ₄ ·10H ₂ O;

Further, the water exchange ratio described in step 3 is greater than 60%;

Further, the pH and temperature measurement described in the eighth step is carried out by using a portable water quality multi-parameter measuring instrument.

The device for measuring the influence of various factors on the biological activity of the wastewater anaerobic treatment process adopts multiple sets of reaction devices arranged in parallel to test the reaction activity under different conditions at the same time. The device is simple, the operation method is convenient, and the airtightness is good. Measure the gas production rate and gas production volume, and combine with other indicators to clarify the influence of various factors on the biological activity of wastewater anaerobic process.

The test method of the invention has the advantages of convenient operation, small error and reliable test result, and can test the rule of influence of various factors on the biological activity of the wastewater anaerobic process.

Description of drawings

Fig. 1 is the device structural representation of measuring pollution factor of the present invention to anaerobic process biological activity influence rule;

FIG. 2 is a schematic structural view of the reaction device 1 .

In the figure: 1 is a reaction device, 1-1 is a reaction vessel, 1-2 is a sealing cover, 1-3 is an air inlet pipe, and 1-4 is an air outlet pipe;

2 is an oxygen removal device, 2-1 is a nitrogen cylinder, 2-2 is a nitrogen cabinet, and 2-3 is a rotor flow control meter;

3 is a gas collection device, 3-1 is a gas flow meter, and 3-2 is an air collection bag;

4 is an oscillating device.

Detailed ways

The present invention will be further described below in conjunction with specific examples.

Embodiment 1: a kind of measuring pollution factor of the present embodiment affects the biological activity of anaerobic process to the device of law, by the reaction device 1 of 6 sets of parallel arrangement, deaeration device 2, gas collection device 3 and shaker 4;

Wherein 6 sets of reaction devices 1 arranged in parallel have the same structure; each set of reaction device 1 includes a volume of 1000mL reaction vessel 1-1, sealing cover 1-2, sealing gasket 1-5 and clamp 1-6; sealing gasket 1 -5 is set between the reaction vessel 1-1 and the sealing cover 1-2, the clamp 1-6 is used to clamp the sealing cover 1-2 and the reaction vessel 1-1; the air inlet 1-3 is arranged on the sealing cover And the air outlet 1-4; the air inlet 1-3 is located in the center of the sealing cover and extends to the bottom of the digestion bottle;

Deoxygenation device 2 is made up of nitrogen cylinder 2-1, nitrogen cabinet 2-2 and a plurality of rotameters 2-3; Nitrogen cylinder 2-1 is connected with the air inlet of nitrogen cabinet 2-2; Nitrogen cabinet 2- The gas outlet of 2 is connected with the gas inlet 1-3 of reaction device 1 through porous gas splitter and rotameter 2-3, and is used for blowing off;

The gas collection device 3 is composed of a gas flowmeter 3-1 and a gas collection bag 3-2; the gas outlet 1-4 is connected to the gas collection bag 3-2 through the gas flowmeter 3-1, and is used to collect the gas generated by the reaction;

The reaction vessels 1 - 1 of the 6 sets of reaction devices 1 arranged in parallel are all fixed on the shaker 4 .

Utilize the device measurement pollution factor of embodiment 1 to the method for the biological activity of anaerobic process, wherein pollution factor is different concentration sulfate, and the method is carried out as follows:

1. Number multiple sets of reaction devices 1 arranged in parallel, respectively No. 0, No. 1, No. 2, No. 3, No. 4 and No. 5, and the volume of the reactor is 500mL;

According to COD concentration of 500mg/L, ammonia nitrogen concentration of 200mg/L, nitrous nitrogen concentration of 0mg/L, nitrate nitrogen concentration of 0mg/L, alkalinity of 500mg/L and total phosphorus concentration of 15mg/L, the 468.60mg/L L of C ₆ H ₁₂ O ₆ , 942.86mg/L of (NH ₄ ) ₂ SO ₄ , 839.16mg/L of NaHCO ₃ and 65.81mg/L of KH ₂ PO ₄ were added to water to dissolve, and then added the necessary for microbial growth Inorganic salts and trace elements, wherein inorganic salts are 150mg/L MgSO ₄ ·7H ₂ O and 68mg/L CaCl ₂ ; trace elements are 5mg/L FeSO ₄ , 0.43mg/L ZnSO ₄ ·7H ₂ O, 0.24 mg/L of CoCl ₂ ·6H ₂ O, 0.99 mg/L of MnCl ₂ ·4H ₂ O, 0.25 mg/L of CuSO ₄ ·5H ₂ O, 0.22 mg/L of Na ₂ MoO ₄ ·2H ₂ O, 0.19 mg/L of NiCl ₂ ·6H ₂ O and 0.21 mg/L of Na ₂ SeO ₄ ·10H ₂ O;

Wash the sludge to be inoculated 3 times with water distribution, and inoculate evenly into each reaction vessel 1-1, control the sludge concentration to 8000mg/L, and the volume and concentration of the mud-water mixture in each reaction vessel 1-1 are equal;

2. Take 5 parts of 500mL solution from the distribution water, add sodium sulfate to it respectively until the sulfate concentration is 50mg/L, 100mg/L, 200mg/L, 400mg/L and 800mg/L, and mix well;

3. Change the water of No. 0 reaction vessel 1-1 by using the original water according to the set water change ratio, and set it as a blank control; use the solution containing pollution factors according to the water change ratio of 60% to change the water of No. 1 and 2 respectively. No., No. 3, No. 4 and No. 5 reaction vessels 1-1 to change the water, filter the mud-water mixture, record the sampling time and measure the pH and temperature at the same time, collect the filtrate to measure the corresponding initial pollution indicators, and pour the sludge back into the reactor ;Initial pollution indicators refer to organic matter concentration [COD] _Inf. , ammonia nitrogen concentration [NH ₄ ⁺ -N] _Inf. , nitrous nitrogen concentration [NO ₂ ^- -N] _Inf. , nitrate nitrogen concentration [NO ₃ ^- -N] _Inf . .and total nitrogen concentration [TN] _{Inf .;}

4. Cover the sealing cover 1-2 of the reaction vessel 1-1, connect the air inlet 1-3 with the oxygen removal device 2 with a trachea, open the oxygen removal device 2, and adjust each rotor flow control meter 2-3 to The nitrogen flow rate is controlled at 1 L/min, and the air stripping is performed for 5 minutes; after the air stripping is completed, firstly close each rotor flow control meter 2-3, then close the oxygen removal device 2, and unplug the trachea at the air inlet 1-3;

5. Connect the gas outlet 1-4 with the gas collecting device 3 with a gas pipe, and clear the gas flow meter 3-1;

6. Turn on the shaking table 4, set the rotating speed of the shaking table 4 to 200rpm, set the temperature to 30°C, and set the shaking reaction time to 20h;

7. After the oscillation reaction is over, close the valve of the gas collection bag 3-2, record the corresponding data of the gas flow meter 3-1, remove the gas composition to be measured from the gas collection bag, and unplug the trachea at the gas outlet 1-4;

Eight, open the sealing cover 1-2 of the reaction vessel 1-1, take the mud-water mixture to filter and measure the pH and temperature, collect the filtrate to measure the corresponding end pollution index, and the sludge is poured back into the reactor; after the settlement is completed, use the water distribution to react respectively The sludge in the container 1-1 is washed 1 to 2 times; the corresponding end pollution indicators refer to the concentration of organic matter [COD] _Eff. , the concentration of ammonia nitrogen [NH ₄ ⁺ -N] _Eff. , the concentration of nitrous [NO ₂ ^- -N] _Eff. , nitrate nitrogen concentration [NO ₃ ^- -N] _Eff. and total nitrogen concentration [TN] _Eff .;

9. Repeat step 2 to step 8 to cycle, cycle 1 cycle per day, cycle 30 days, after the cycle is over, respectively calculate the COD removal rate, COD removal load, ammonia nitrogen removal rate, total nitrogen removal rate and total nitrogen removal rate according to the following formula The load is calculated:

In the formula, t is the reaction time (h);

Obtain the COD removal rate, COD removal load, ammonia nitrogen removal rate, total nitrogen removal rate and total nitrogen removal load corresponding to various pollution factors. .

The results of data processing showed that compared with No. 0 blank control group, when No. 1, No. 2, No. 3, No. 4 and No. 5 reactors were exposed to concentrations of 50mg/L, 100mg/L, 200mg/L, 400mg/L L and 800mg/L of sulfate, there is no significant change in each reactor, indicating that sulfate does not show a strong inhibitory effect on the anaerobic digestion process of microorganisms in this concentration range.

Embodiment 2: Utilize the device measurement pollution factor of embodiment 1 to the method for anaerobic process biological activity influence law, wherein pollution factor is different concentration erythromycin, and this method is carried out as follows:

1. Number the six sets of reaction devices 1 arranged in parallel, respectively No. 0, No. 1, No. 2, No. 3, No. 4 and No. 5, and the volume of the reactor is 1000mL;

According to COD concentration of 0mg/L, ammonia nitrogen concentration of 250mg/L, nitrous nitrogen concentration of 200mg/L, nitrate nitrogen concentration of 0mg/L, alkalinity of 1600mg/L and total phosphorus concentration of 10mg/L, the 1178.57mg/L Add L of (NH ₄ ) ₂ SO ₄ , 985.71mg/L of NaNO ₂ , 2685.31mg/L of NaHCO ₃ and 43.87mg/L of KH ₂ PO ₄ into water to dissolve, then add the necessary inorganic salts and trace amounts of microbial growth Elements; inorganic salts are 150mg/L MgSO ₄ 7H ₂ O and 68mg/L CaCl ₂ ; trace elements are 5mg/L FeSO ₄ , 0.43mg/L ZnSO ₄ 7H ₂ O, 0.24mg/L CoCl ₂ 6H ₂ O, 0.99 mg/L MnCl ₂ 4H ₂ O, 0.25 mg/L CuSO ₄ 5H ₂ O, 0.22 mg/L Na ₂ MoO ₄ 2H ₂ O, 0.19 mg/L NiCl ₂ ·6H ₂ O and 0.21 mg/L of Na ₂ SeO ₄ ·10H ₂ O;

Wash the sludge to be inoculated with water for 3 times, and inoculate evenly into each reaction vessel 1-1, control the sludge concentration to 6000 mg/L, and the volume and concentration of the mud-water mixture in each reaction vessel 1-1 are equal;

2. Take 5 parts of 1000mL water, add erythromycin to them respectively until the concentration of erythromycin is 0.001mg/L, 1mg/L, 10mg/L, 50mg/L and 100mg/L, and mix well to get 5 kinds Solutions containing pollutants;

3. Change the water of No. 0 reaction vessel 1-1 with the original water according to the set water change ratio, and set it as a blank control; use the solution containing pollution factors according to the water change ratio of 70% to change the water of No. 1 and 2 respectively. No., No. 3, No. 4 and No. 5 reaction vessels 1-1 to change the water, filter the mud-water mixture, record the sampling time and measure the pH and temperature at the same time, collect the filtrate to measure the corresponding initial pollution indicators, and pour the sludge back into the reactor ;Initial pollution indicators refer to ammonia nitrogen concentration [NH ₄ ⁺ -N] _Inf. , nitrous nitrogen concentration [NO ₂ ^- -N] _Inf. , nitrate nitrogen concentration [NO ₃ ^- -N] _Inf. and total nitrogen concentration [TN] _Inf .;

4. Cover the sealing cover 1-2 of the reaction vessel 1-1, connect the air inlet pipe 1-3 with the oxygen removal device 2 with a trachea, open the oxygen removal device 2, and adjust the rotor flow control meters 2-3 to transfer the nitrogen gas Control the flow rate at 1L/min, and blow off for 5 minutes; after the blow off is completed, first turn off the rotor flow control meters 2-3, then turn off the oxygen removal device 2, and unplug the air pipes at the inlet pipes 1-3;

5. Connect the outlet pipe 1-4 with the gas collecting device 3 with a gas pipe, and clear the gas flow meter 3-1;

6. Turn on the shaking table 4, set the rotating speed of the shaking table 4 to 180rpm, set the temperature to 25°C, and set the shaking reaction time to 10h;

7. After the oscillation reaction is over, close the valve of the gas collection bag 3-2, record the corresponding data of the gas flow meter 3-1, remove the gas composition to be measured from the gas collection bag, and unplug the trachea at the outlet pipe 1-4;

Eight, open the sealing cover 1-2 of the reaction vessel 1-1, take the mud-water mixture to filter and measure the pH and temperature, collect the filtrate to measure the corresponding end pollution index, and the sludge is poured back into the reactor; after the settlement is completed, use the water distribution to react respectively The sludge in the container 1-1 is washed 1 to 2 times; the corresponding end pollution indicators refer to the concentration of ammonia nitrogen [NH ₄ ⁺ -N] _Eff. , the concentration of nitrous [NO ₂ ^- -N] _Eff. , the concentration of nitrate nitrogen [NO ₃ ^-- N] _Eff. and total nitrogen concentration [TN] _Eff .;

9. Repeat step 2 to step 8 to cycle, cycle 1 cycle per day, and cycle for 44 days. After the cycle is over, the ammonia nitrogen removal rate, nitrous nitrogen removal rate, nitrate nitrogen removal rate, total nitrogen removal rate and total The nitrogen removal load is calculated as:

In the formula, t is the reaction time (h);

Obtain the relationship of ammonia nitrogen removal rate, nitrous nitrogen removal rate, nitrate nitrogen removal rate, total nitrogen removal rate and total nitrogen removal load over time corresponding to various pollution factors, and combine pH and temperature changes to obtain various pollution factors Effects on biological activity of anaerobic process.

The data processing results showed that compared with the No. 0 blank control group, the treatment effects of No. 1 and No. 2 reactors were all reduced first and then recovered, indicating that the anammox bacteria were first inhibited and then inhibited under the action of low-concentration antibiotic erythromycin. Showed adaptability; No. 3, No. 4 and No. 5 reactors were all inhibited compared with No. 0 blank control group and did not recover in the later stage, indicating that high concentrations of antibiotic erythromycin (10-100mg/L) have an effect on Ammonium oxidizing bacteria have a strong inhibitory effect, and gradually increase with the concentration, the inhibition threshold is 10mg/L.

Claims (10)

1. a device for determining the influence rule of pollution factors on the biological activity of an anaerobic process is characterized by comprising a plurality of sets of reaction devices (1), a deoxidizing device (2), a gas collecting device (3) and an oscillating device (4) which are arranged in parallel;

Wherein the structures of a plurality of sets of reaction devices (1) which are arranged in parallel are the same; each set of reaction device (1) comprises a reaction container (1-1) and a sealing cover (1-2); the sealing cover is provided with an air inlet (1-3) and an air outlet (1-4);

the deaerating device (2) comprises a nitrogen cylinder (2-1), a nitrogen cabinet (2-2) and a plurality of rotor flow controllers (2-3); the nitrogen cylinder (2-1) is connected with the air inlet of the nitrogen cabinet (2-2); an air outlet of the nitrogen gas cabinet (2-2) and the rotor flow control meter (2-3) are connected with an air inlet (1-3) of the reaction device (1) and used for stripping;

The gas collecting device (3) comprises a gas flowmeter (3-1) and a gas collecting bag (3-2); the gas outlet (1-4) is connected with the gas collecting bag (3-2) through a gas flowmeter (3-1) and is used for collecting gas generated by reaction;

Reaction containers (1-1) of a plurality of sets of reaction devices (1) which are arranged in parallel are all fixed on an oscillating device (4).

2. The apparatus for determining the influence law of pollution factors on the biological activity of an anaerobic process as claimed in claim 1, wherein the reaction apparatus (1) further comprises a sealing washer (1-5) and a clamp (1-6), the sealing washer (1-5) is disposed between the reaction vessel (1-1) and the sealing cover (1-2), and the clamp (1-6) is used for clamping the sealing cover (1-2) and the reaction vessel (1-1).

3. The apparatus for determining the law of influence of polluting elements on the biological activity of anaerobic processes according to claim 1 or 2, wherein said air inlet (1-3) is located in the central position of the sealing cover and extends to the bottom of the digestion vessel.

4. The device for determining the influence law of pollution factors on the biological activity of an anaerobic process as claimed in claim 1 or 2, wherein pneumatic check valves are arranged at the air outlets (1-4) and the air inlets (1-3).

5. The device for determining the law of the impact of polluting elements on the biological activity of anaerobic processes according to claim 1 or 2, characterized in that a porous gas splitter is provided between the nitrogen tank (2-2) and the plurality of rotameter (2-3).

6. the device for determining the influence rule of the pollution factors on the biological activity of the anaerobic process according to the claim 1 or 2, characterized in that 3-10 sets of the reaction devices (1) are arranged in parallel.

7. an apparatus for determining the law of influence of polluting elements on the biological activity of anaerobic processes according to claim 1 or 2, characterised in that the oscillating means (4) is a rocking platform.

8. A method for determining the law of the effect of polluting elements on the biological activity of anaerobic processes using the device of claim 1, characterized in that it comprises the following steps:

Numbering a plurality of sets of reaction devices (1) which are arranged in parallel, wherein the numbers are No. 0, No. 1, No. 2, No. … … and No. n, washing sludge to be inoculated for 2-3 times by adopting water distribution, uniformly inoculating the sludge to each reaction container (1-1), controlling the sludge concentration to be 5000-10000 mg/L, and enabling the volume and the concentration of sludge-water mixed liquor in each reaction container (1-1) to be equal;

taking n equal parts of water, adding various pollutant substances into the water respectively, and uniformly mixing to obtain n solutions containing the pollutants;

Thirdly, changing water by adopting water distribution according to a set water changing ratio and setting the water changing ratio as a blank control in a No. 0 reaction container (1-1); changing water of the solution containing the pollution factors according to a set water change ratio and other reaction containers (1-1) with corresponding numbers, taking a muddy water mixed solution for filtration, simultaneously recording sampling time, measuring pH and temperature, collecting filtrate to measure corresponding initial pollution indexes, and pouring the sludge into the reactor; the initial pollution index is organic matter Concentration (COD)]_Inf.Ammonia nitrogen concentration [ NH ]₄ ⁺-N]_Inf.Nitrogen concentration [ NO ]₂ ^--N]_Inf.Nitrate nitrogen concentration [ NO₃ ^--N]_Inf.and/or total nitrogen concentration [ TN]_Inf.；

Fourthly, covering a sealing cover (1-2) of the reaction container (1-1), connecting an air inlet (1-3) with a deaerating device (2) by using an air pipe, opening the deaerating device (2), adjusting each rotor flow control meter (2-3) to control the nitrogen flow rate to be 0.5-1.5L/min, and blowing off for 5-10 min; after blowing off is finished, closing the rotor flow control meters (2-3), closing the deaerating device (2) and pulling out the air pipes at the air inlets (1-3);

connecting the gas outlet (1-4) with the gas collecting device (3) by using a gas pipe, and resetting the gas flowmeter (3-1);

Sixthly, turning on the oscillation device (4), setting the rotation speed of the oscillation device (4) to be 100-300 rpm, setting the temperature to be 25-35 ℃, and setting the oscillation reaction time to be 10-20 h;

Seventhly, after the oscillation reaction is finished, closing a valve of the gas collecting bag (3-2), recording corresponding data of the gas flowmeter (3-1), taking down gas components to be detected of the gas collecting bag (3-2), and pulling out a gas pipe at a gas outlet (1-4);

Eighthly, opening a sealing cover (1-2) of the reaction container (1-1), taking the muddy water mixed liquor, filtering, measuring the pH value and the temperature, collecting the filtrate, measuring the pollution index after the filtrate is correspondingly measured, and pouring the sludge into the reactor; after the sedimentation is finished, washing the sludge in the reaction container (1-1) for 1-2 times by water distribution; the corresponding index of ending pollution is the concentration of organic matter (COD)]_Eff.ammonia nitrogen concentration [ NH ]₄ ⁺-N]_Eff.nitrogen concentration [ NO ]₂ ^--N]_Eff.nitrate nitrogen concentration [ NO₃ ^--N]_EffAnd/or total nitrogen concentration [ TN]_Eff.；

And ninthly, repeating the steps two to eight for circulation, wherein 1-2 periods are circulated every day, the circulation lasts for at least 30 days, and after the circulation is finished, calculating the COD removal rate, the COD removal load, the ammonia nitrogen removal rate, the nitrous removal rate, the nitric nitrogen removal rate, the total nitrogen removal rate and/or the total nitrogen removal load according to the following formulas respectively:

Wherein t is reaction time in hours;

And obtaining the change relationship of the COD removal rate, the COD removal load, the ammonia nitrogen removal rate, the nitrous oxide removal rate, the nitric nitrogen removal rate, the total nitrogen removal rate and/or the total nitrogen removal load corresponding to various pollution factors along with time, and obtaining the influence rule of various pollution factors on the biological activity of the anaerobic process.

9. The method for determining the rule of influence of pollution factors on the biological activity of an anaerobic process according to claim 8 by using the device of claim 1, wherein the water distribution in the second step is prepared by the following method: according to the COD concentration of 0-5000 mg/L, the ammonia nitrogen concentration of 0-600 mg/L, the nitrite concentration of 0-300 mg/L, the nitrate nitrogen concentration of 0-300 mg/L, the alkalinity of 0-2000 mg/L and the total phosphorus concentration of 0-20 mg/L, 0-4686.04 mg/L of C₆H₁₂O₆0 to 2828.57mg/L of (NH)₄)₂SO₄0 to 1478.57mg/L NaNO₂0 to 1821.43mg/L NaNO₃0-3356.64 mg/L NaHCO₃and KH of 0-87.74 mg/L₂PO₄Adding water to dissolve, and adding inorganic salt and trace elements required by microorganism growth; wherein the inorganic salt is MgSO 100-200 mg/L₄·7H₂o and 50-100 mg/L CaCl₂(ii) a FeSO with 4-6 mg/L of trace elements₄0.3-0.5 mg/L ZnSO₄·7H₂O, 0.1-0.3 mg/L CoCl₂·6H₂O, 0.5-1.5 mg/L MnCl₂·4H₂0.1-0.3 mg/L of CuSO₄·5H₂o, 0.1-0.3 mg/L Na₂MoO₄·2H₂O, 0.1-0.3 mg/L NiCl₂·6H₂O and 0.1-0.3 mg/L of Na₂SeO₄·10H₂O。

10. The method for determining the influence of pollution factors on the biological activity of an anaerobic process according to claim 8 or 9 by using the device of claim 1, wherein the water change ratio in the third step is more than 60%.