CN110560249A - Method for quickly separating mosquito pupae - Google Patents
Method for quickly separating mosquito pupae Download PDFInfo
- Publication number
- CN110560249A CN110560249A CN201910703749.5A CN201910703749A CN110560249A CN 110560249 A CN110560249 A CN 110560249A CN 201910703749 A CN201910703749 A CN 201910703749A CN 110560249 A CN110560249 A CN 110560249A
- Authority
- CN
- China
- Prior art keywords
- pupae
- larvae
- mosquito
- pupa
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255925 Diptera Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 241000382353 Pupa Species 0.000 claims abstract description 21
- 239000005457 ice water Substances 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 19
- 241000256113 Culicidae Species 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000000151 deposition Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 7
- 239000011148 porous material Substances 0.000 description 5
- 241000256173 Aedes albopictus Species 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000005667 attractant Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 230000002940 repellent Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
- B03B5/28—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation
- B03B5/30—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation using heavy liquids or suspensions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
the invention discloses a method for quickly separating mosquito pupae. The method comprises the steps of pouring a mixture of mosquito larvae and pupae into ice water, floating the pupae on the water surface, sinking the larvae into the ice water, and respectively fishing out the upper pupae and the lower pupae to obtain separated pupae and larvae. The inventor finds that when larvae and pupae of mosquitoes are placed in ice water, the larvae automatically sink at the moment of being frozen, the pupae float on the water surface, and when the temperature of the ice water is raised to normal temperature (25 ℃) after the pupae is fished out, the larvae automatically float upwards. Therefore, the mosquito larva and pupa separating device can effectively separate the mosquito larvae and pupae, improves the separation efficiency of the mosquito larvae and pupae, saves time and labor, and does not damage the pupae.
Description
Technical Field
the invention belongs to the field of mosquito control, and particularly relates to a method for quickly separating mosquito pupae.
Background art:
The major harm to mosquitoes is the transmission of disease. Mosquito-transmitted diseases have been studied as many as 80. Killing mosquitoes is an effective way to prevent and reduce diseases. In the experiment, a large amount of mosquitoes are needed to carry out researches on attractants, repellent agents, sterility technologies and the like. Therefore, a large amount of mosquito pupae and later adults need to be obtained for experimental verification.
The invention content is as follows:
The invention aims to provide a method for quickly separating mosquito pupae.
The invention discloses a method for quickly separating mosquito pupae, which comprises the following steps:
Pouring the mixture of mosquito larva and pupa into ice water, floating pupa on water surface, depositing larva in ice water, and respectively fishing out upper pupa and lower pupa to obtain separated pupa and larva.
The collected pupae can be used for pupal stage tests or experimental needs of adult culture.
Preferably, said mixture of mosquito larvae and pupae is obtained by: pouring culture medium or culture solution containing mosquito larva and pupa into screen mesh, filtering with water, and removing the medium to obtain mosquito larva and pupa mixture.
The screen is a 20-mesh screen.
Preferably, the culture medium or culture solution containing the larvae and pupae of the mosquito is poured into a screen, filtered with water, the medium or culture solution is removed to obtain a mixture of the larvae and pupae of the mosquito, the mixture of the larvae and pupae of the mosquito is poured into ice water, the pupae floats on the water surface, the larvae sink in the ice water, then the pupae is collected by a filter spoon with a screen, and the larvae on the lower layer are collected to obtain separated pupae and larvae.
the inventor finds that when larvae and pupae of mosquitoes are placed in ice water, the larvae automatically sink at the moment of being frozen, the pupae float on the water surface, and when the temperature of the ice water is raised to normal temperature (25 ℃) after the pupae is fished out, the larvae automatically float upwards.
Therefore, the mosquito larva and pupa separating device can effectively separate the mosquito larvae and pupae, improves the separation efficiency of the mosquito larvae and pupae, saves time and labor, and does not damage the pupae.
description of the drawings:
FIG. 1 is a graph showing the effect of separation of mosquito larvae and pupae.
The specific implementation mode is as follows:
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1:
pouring a culture medium containing larvae and pupae of mosquitoes for a raised experiment into a filter cup with a 20-mesh sieve pore on the bottom wall, filtering with water, removing the medium (filtering the medium out of the sieve pore) to obtain a mixture of the larvae and pupae of the mosquitoes, placing the filter cup containing the mixture of the larvae and pupae of the mosquitoes into ice water, enabling the ice water to enter the filter cup through the sieve pore, waiting for 10 seconds, enabling the pupae to float on the water surface, enabling the larvae to sink in the ice water, collecting the pupae with a filter spoon with a screen, lifting the filter cup after collection, and obtaining the larvae at the bottom of the filter cup. The collected pupae are living bodies and can be hatched into imagoes, so that the pupae can be used for pupal stage experiments or adult culture experiments.
Comparison: the mixture of the larvae and pupae of the mosquitoes is poured into water at normal temperature (25 ℃), the larvae and pupae of the mosquitoes float on the water surface, and the larvae of the mosquitoes do not sink into the water or the water bottom.
example 2:
Feeding 200 aedes albopictus pupas and 200 aedes albopictus larvae in a culture solution mixed in a water pan, then pouring the mixture into a filter cup with a 20-mesh sieve pore on the bottom wall, flushing and filtering the filter cup with water, removing the culture solution to obtain a mixture of the larvae and the pupas of the mosquitoes, placing the filter cup containing the mixture of the larvae and the pupas of the mosquitoes into ice water, enabling the ice water to enter the filter cup through the sieve pore, waiting for 10 seconds, enabling the pupas to float on the water surface, enabling the larvae to sink in the ice water, collecting the pupas by using a filter spoon with a screen, lifting the filter cup after collection is finished, and obtaining the larvae at the bottom of the filter cup. The collected pupae are living bodies and can be hatched into imagoes, so that the pupae can be used for pupal stage experiments or adult culture experiments.
The results of the experiment are shown in FIG. 1, and it can be seen from FIG. 1 that the larvae and pupae are well separated.
Example 3:
The mixture of larvae and pupae of aedes albopictus of example 2 was poured into ice water and after waiting 10 seconds, the pupae floated on the water surface while the mosquito larvae settled in the water.
Comparison: the mixture of larvae and pupae of Aedes albopictus was poured into water at room temperature (25 deg.C) to allow both larvae and pupae of mosquito to float on the water surface without sinking into the water.
Claims (4)
1. A method for rapidly separating mosquito pupae is characterized by comprising the following steps:
Pouring the mixture of mosquito larva and pupa into ice water, floating pupa on water surface, depositing larva in ice water, and respectively fishing out upper pupa and lower pupa to obtain separated pupa and larva.
2. the method of claim 1, wherein said mixture of mosquito larvae and pupae is obtained by: pouring culture medium or culture solution containing mosquito larva and pupa into screen mesh, filtering with water, and removing the medium to obtain mosquito larva and pupa mixture.
3. The method of claim 2, wherein the screen is a 20 mesh screen.
4. The method of claim 1, wherein the culture medium or culture solution containing the larvae and pupae of the mosquito is poured into a sieve, filtered with water, and the medium or culture solution is removed to obtain a mixture of the larvae and pupae of the mosquito, the mixture of the larvae and pupae of the mosquito is poured into ice water, the pupae float on the water surface, the larvae sink in the ice water, then the pupae are collected with a filtering spoon with a sieve, and the larvae in the lower layer are collected to obtain separated pupae and larvae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910703749.5A CN110560249A (en) | 2019-07-31 | 2019-07-31 | Method for quickly separating mosquito pupae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910703749.5A CN110560249A (en) | 2019-07-31 | 2019-07-31 | Method for quickly separating mosquito pupae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110560249A true CN110560249A (en) | 2019-12-13 |
Family
ID=68773879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910703749.5A Pending CN110560249A (en) | 2019-07-31 | 2019-07-31 | Method for quickly separating mosquito pupae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110560249A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118058237A (en) * | 2024-03-26 | 2024-05-24 | 中山大学 | Separation method of male and female mosquito pupae |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651414A (en) * | 2005-01-18 | 2005-08-10 | 上海应用技术学院 | Separation and purification method of pyriproxyfen |
| CN102499182A (en) * | 2011-10-19 | 2012-06-20 | 武汉市疾病预防控制中心 | Integral type mosquito raising device |
| CN202285912U (en) * | 2011-10-19 | 2012-07-04 | 武汉市疾病预防控制中心 | Integrated mosquito-raising apparatus |
| CN102910737A (en) * | 2012-10-20 | 2013-02-06 | 北京工业大学 | Device and method for culturing and separating chironomus larvas for reducing sludge |
| CN103340179A (en) * | 2013-06-25 | 2013-10-09 | 广东省农业科学院植物保护研究所 | Phyllotreta striolata insect pest situation investigation method and special separation device |
| US9180464B2 (en) * | 2012-03-20 | 2015-11-10 | Oxitec Limited | Sorting apparatus for arthropods and method of use thereof |
| JP5824450B2 (en) * | 2009-07-14 | 2015-11-25 | インベスティガシオン・イ・コメルシアリサシオン・ビオテクノロヒカ・オメビット・ソシエダッド・アノニマ | Methods for obtaining fatty acids from insect larvae |
| CN208879055U (en) * | 2018-09-30 | 2019-05-21 | 湖北远志黄粉虫养殖有限责任公司 | A kind of screening plant of worm pupa for separating yellow meal worm, larva and frass |
-
2019
- 2019-07-31 CN CN201910703749.5A patent/CN110560249A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651414A (en) * | 2005-01-18 | 2005-08-10 | 上海应用技术学院 | Separation and purification method of pyriproxyfen |
| JP5824450B2 (en) * | 2009-07-14 | 2015-11-25 | インベスティガシオン・イ・コメルシアリサシオン・ビオテクノロヒカ・オメビット・ソシエダッド・アノニマ | Methods for obtaining fatty acids from insect larvae |
| CN102499182A (en) * | 2011-10-19 | 2012-06-20 | 武汉市疾病预防控制中心 | Integral type mosquito raising device |
| CN202285912U (en) * | 2011-10-19 | 2012-07-04 | 武汉市疾病预防控制中心 | Integrated mosquito-raising apparatus |
| US9180464B2 (en) * | 2012-03-20 | 2015-11-10 | Oxitec Limited | Sorting apparatus for arthropods and method of use thereof |
| CN102910737A (en) * | 2012-10-20 | 2013-02-06 | 北京工业大学 | Device and method for culturing and separating chironomus larvas for reducing sludge |
| CN103340179A (en) * | 2013-06-25 | 2013-10-09 | 广东省农业科学院植物保护研究所 | Phyllotreta striolata insect pest situation investigation method and special separation device |
| CN208879055U (en) * | 2018-09-30 | 2019-05-21 | 湖北远志黄粉虫养殖有限责任公司 | A kind of screening plant of worm pupa for separating yellow meal worm, larva and frass |
Non-Patent Citations (1)
| Title |
|---|
| 杨俊德等: "实验室斯氏按蚊的饲养方法", 《医学动物防制》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118058237A (en) * | 2024-03-26 | 2024-05-24 | 中山大学 | Separation method of male and female mosquito pupae |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant before: Guangdong Institute of Applied Biological Resources |
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| CB02 | Change of applicant information | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191213 |
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| WD01 | Invention patent application deemed withdrawn after publication |