CN110527643A - One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation - Google Patents
One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation Download PDFInfo
- Publication number
- CN110527643A CN110527643A CN201910552737.7A CN201910552737A CN110527643A CN 110527643 A CN110527643 A CN 110527643A CN 201910552737 A CN201910552737 A CN 201910552737A CN 110527643 A CN110527643 A CN 110527643A
- Authority
- CN
- China
- Prior art keywords
- psychrobacter
- soil
- plant
- culture
- low temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Processing Of Solid Wastes (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of one plant of soil Psychrobacter and its in the application of agriculture low temperature aerobic composting fermentation, the bacterial strain is soil Psychrobacter, isolated strains are named as soil Psychrobacter DL1901(Psychrobacter sp.SOD-DL1901), China typical culture collection center is preserved on June 6th, 2019, the deposit date is on June 6th, 2019, deposit number was CCTCC NO:M 2019443.The problems such as agricultural solid waste compost aerobic fermentation starting is slow, and time-consuming, high production cost under cryogenic conditions is solved, can be fermented with quick start under low temperature environment, the temperature range of the Psychrobacter being rapidly heated, the bacterial strain starting aerobic fermentation is 5-25 DEG C.
Description
Technical field
The invention belongs to field of agricultural microbial technology, and in particular to one plant of soil Psychrobacter and its low in agricultural solid waste
The application of warm aerobic composting fermentation.
Background technique
Aerobic fermentation is a complicated biochemical reaction process, is by organic matter such as (cellulose, hemicellulose, wood
Quality, protein, amino acid, fat etc.) it is decomposed into inorganic matter, the process to mineralize.The microbial process of low temperature aerobic fermentation
Be broadly divided into four-stage: first stage, low temperature bacterial strain (such as Bacillus) under cryogenic, pass through special physiology machine
System degradation sugar, starch, the simple organic matter such as amino acid, and improve compost temperature, thus start medium temperature bacterial strain (such as zymophyte,
Fungi etc.)；Second stage, medium temperature bacterial strain are continued point using soluble and degradable organic matter as nutrition and energy source
Organic matter is solved, is proliferated rapidly, and cooperate with a variety of enzyme decomposing proteins, part hemicellulose and cellulose, lignin, releases simultaneously
Amplification quantity heat, so that compost temperature be made constantly to rise, high temperature bacterial strain is activated (such as actinomyces, fungi)；It is phase III, high
Warm bacterium generates a variety of enzymes such as a large amount of extracellular amylase, fire resistant alpha-diastase, cellulase and extracellular proteinase after being activated, have
Machine object is largely degraded, and humus initially forms, and hot stage, which plays, effectively kills pathogen, helminth, grass-seed in solid waste,
Large quantity of moisture is evaporated, the important function such as decomposed are accelerated.When temperature reaches 70 DEG C or more, microorganism mortality or be in suspend mode
State, under the action of various enzymes, organic matter continues to decompose, and heat can disappear due to the death or suspend mode of microorganism, the effect of enzyme
It moves back and gradually decreases, when temperature is down to 50 DEG C or so, mesophile is active again, decomposes the objects such as remaining cellulose
Matter；Fourth stage, the decomposed stage, in high temperature latter stage, the humus for being only left the organic matter of the more difficult decomposition in part and newly being formed, this
When microbial activity decline, heat reduce, temperature decline, medium temperature bacterial strain again it is active, to the organic of remaining more difficult decomposition
Object further decomposes, and humus is increasing and tending towards stability, and fermentation enters the decomposed stage at this time.
China is large agricultural country, animal husbandry big country, and the harmless treatment of feces of livestock and poultry has become extremely urgent problem.I
State's crop acreage is huge, plant straw abundance.Plant straw is a kind of biomass money rich in gas chromatography
Source, but China is low to straw producing level, 70% or so directly abandons and burns, serious waste is not only caused in resource, and
And bring serious environmental pollution.China's agricultural solid waste 70% is feces of livestock and poultry, and feces of livestock and poultry is that a kind of carbon-nitrogen ratio is lower, is contained
A large amount of organic matters and the source the N donor compared with juicy, and there is faster transformation efficiency.Feces of livestock and poultry is mostly Natural compost, production
Biogas etc., but actual effect and ideal not to the utmost.It is existing in improvement, the transformation that the whole world is used for soil using feces of livestock and poultry as fertilizer
Long history, the crop growths institute such as N, P, K rich in is required in the feces of livestock and poultry after decomposed fermentation process
Nutriment, while the usage amount of chemical fertilizer can also be reduced, the pollution to environment be reduced to a certain extent, to soil
It destroys.The harmless treatment of agricultural solid waste aerobic fermentation has vast potential for future development, obtained global extensive concern with
Using, aerobic fermentation can make agricultural solid waste raw material, the certain temperature, fungus strain the effects of under be converted into inorganic matter, solve agriculture
The recycling problem of industry solid waste, can also improve soil.But starts aerobic fermentation relative difficulty under cryogenic conditions, be in fermentation process
There are still a Universal Problems, it is long that this allows for fermentation period, and turnover is slow, high production cost.Aerobic fermentation is a pole
Its complicated aerobic digestion process is that various fungus strains act synergistically as a result, addition is suitable in low temperature aerobic fermentation processes
Pyschrophile has great meaning for improving fermentation period.Finding is suitable for microorganism raw under cryogenic, optimization fermentation
Condition to solving the problem of that northern China cold season region aerobic fermentation period long low efficiency is of great significance, and has
Considerable economic benefit.
Summary of the invention
In order to solve the problems such as agricultural solid waste compost aerobic fermentation starting is slow, and time-consuming, high production cost under cryogenic conditions,
The present invention provides a kind of low temperature resistant, can be fermented with quick start under low temperature environment, the Psychrobacter being rapidly heated, cryogenic conditions
Under, improving livestock excrement composting aerobic fermentation temperature is key problem in technology, and can it decides rapidly enter the megathermal period and influence to send out
The innoxious process of ferment and reaction time.Low temperature bacterial strain passes through special physiological mechanism degradation sugar, starch etc. under cryogenic
Simple organic matter simultaneously improves compost temperature, to start medium temperature bacterial strain, then high temperature bacterial strain mass propagation, completes all to decompose
Decomposed process.
Present invention technical solution used for the above purpose is: one plant of soil Psychrobacter, and the bacterial strain is soil
Earth Psychrobacter, isolated strains are named as soil Psychrobacter DL1901(Psychrobacter sp.SOD-DL1901), in
On June 6th, 2019 is preserved in China typical culture collection center, preservation address: Wuhan, China, Wuhan University；Preservation date
For on June 6th, 2019, deposit number was CCTCC NO:M 2019443.
Application of the above-mentioned soil Psychrobacter in the aerobic composting fermentation of agricultural solid residue.
Wherein, the screening mode of the bacterial strain are as follows: sample is taken, sterile saline is added with the ratio of 100ml/10g,
Shaking table 150r/min, concussion, for dilution spread in primary dcreening operation culture medium Low- temperature culture, picking grows bacterium colony switching pure culture.
Wherein, primary dcreening operation culture medium are as follows: dipotassium hydrogen phosphate 0.1%, cysteine 0.025%, yeast powder 0.2%, agar 2%, second
Sour sodium 0.25%, magnesium sulfate 0.01%, glucose 0.2%, soy peptone 0.5%, are prepared with purified water, 7.2,37 DEG C of pH value cultures
24-48h。
Wherein, extremely frigid zones frozen soil layer is acquired, air-dries and obtains sample with mortar grinder powdering after handling.
The invention has the benefit that solving agricultural solid waste compost aerobic fermentation starting under cryogenic conditions, slowly, time-consuming, energy
The problems such as consumption is more, high production cost quick start can ferment at low ambient temperatures, the Psychrobacter being rapidly heated, the bacterial strain
The temperature range for starting aerobic fermentation is 5-25 DEG C.
Detailed description of the invention
Fig. 1 is flat-plate bacterial colony aspect graph.
Fig. 2 is gram stain microscopy result.
Fig. 3 is and correlated series Blast comparison result in Genebank.
Fig. 4 is the Psychrobacter sp.SOD-DL1901 phylogenetic tree based on 16S rDNA sequence.
Fig. 5 is 6 monitoring point ramp cases.
Specific embodiment
Explanation is further explained to the present invention combined with specific embodiments below, it should be understood that these embodiments are merely to illustrate
The present invention and do not limit the scope of the invention, after the present invention has been read, those skilled in the art are to of the invention various etc.
The modification of valence form each falls within the application range as defined in the appended claims.
1, sample acquires: acquisition extremely frigid zones frozen soil layer.
2, sample does air-dried processing.
3, by after air-dry sample mortar grinder powdering, take 10g to 100ml sterile saline, shaking table 150r/min
30min is shaken, i.e. acquisition sample suspension, then successively carry out 10 times of dilutions, dilution is coated on primary dcreening operation culture medium, 10~20
DEG C culture.
5, primary dcreening operation culture medium: dipotassium hydrogen phosphate 0.1%(mass percent, similarly hereinafter), cysteine 0.025%, yeast powder
0.2%, agar 2%, sodium acetate 0.25%, magnesium sulfate 0.01%, glucose 0.2%, soy peptone 0.5%, are prepared with purified water.
6, the micro- protuberance of bacterium is grown in solid medium, mellow and full, surface is smooth, and bacterium colony is moderate, toughness, is in off-white color, no
It is transparent.Gram-positive bacillus, such as Fig. 1-2.
7, picking bacterium to be checked is inoculated in NA fluid nutrient medium, 37 DEG C of shaking overnight incubations respectively.DNA extracts
8,16s gene cloning sequencing primer:
27F: 5-AGAGTTTGATCCTGGCTCAG-3
1492R:5-CTACGGCTACCTTGTTACGA-3
9, pcr amplification reaction system (25ul): genomic DNA 1.0ul, 10 × Buffer (Mg2+ containing 2.5mM) 2.5ul,
Taq polymerase (5u/ μ L) 0.5ul, dNTP(10mM) 1.0ul, 27F (10uM) 0.5ul, 1492R (10uM) 0.5ul,
ddH2O 0.5ul。
10, PCR reaction condition: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s, 58 DEG C of annealing 45s, 72 DEG C of extension 80s, altogether
35 circulations；72 DEG C of extension 7min, last 4 DEG C of Infinite Cyclics.
11, the homology with soil Psychrobacter is searched on GenBank 99.67%.
12, the isolated strains are named as soil Psychrobacter DL-1901(Psychrobacter sp.SOD-DL1901).
13, it is good under the starting cryogenic conditions in agricultural solid residue processing to implement soil Psychrobacter of the invention
Aerobe fermentation.
After the soil Psychrobacter is cultivated for 24 hours in LB liquid medium, agricultural solid is inoculated into the zymophyte for accounting for 10%
In body waste fermentation reaction heap, the starting temperature of this aerobic fermentation is 5~20 DEG C, and piling temperature is 20 DEG C, and 12h is close to 30
DEG C, it can be close to 60 DEG C for 24 hours, 48h maximum temperature reaches nearly 70 DEG C, and substantially reducing aerobic fermentation under cryogenic conditions will pass through
Time required for 4~7d reaches 70 DEG C of maximum temperature.
14, LB culture medium: 5 g of yeast powder/L, peptone 10g/L, NaCl10g/L, agar powder 15g/L, go from
Sub- water 1000mL, pH 7.0.
15, -24 DEG C of ambient temperature, heap body about 490m3.If 6 temp probes, 0-96h temperature monitoring situation see the table below and
Attached drawing 4.
| Time supervision point | 0h | 12h | 24h | 48h | 72h | 96h |
| 1 | 8 | 18 | 49 | 59 | 67 | 72 |
| 2 | 10 | 21 | 48 | 62 | 71 | 75 |
| 3 | 15 | 25 | 50 | 60 | 70 | 73 |
| 4 | 15 | 25 | 72 | 80 | 78 | 79 |
| 5 | 21 | 30 | 68 | 77 | 76 | 85 |
| 6 | 25 | 47 | 52 | 68 | 71 | 72 |
。
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art within the technical scope of the present disclosure, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Sequence table
<110>Dalian Tai Fengchang agricultural science and technology Co., Ltd
<120>one plants of soil Psychrobacters and its application in agricultural solid waste low temperature aerobic fermentation
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1500
<212> DNA
<213>soil Psychrobacter DL1901 (Psychrobacter sp.SOD-DL1901)
<400> 1
ggttaccttg ttacgacttc accccagtca tcaaccacac cgtggtgaac gccttcctta 60
cggttaggct atccacttct ggtgcaatca actcccatgg tgtgacgggc ggtgtgtaca 120
aggcccggga acgtattcac cgcggcattc tgatccgcga ttactagcga ttcctacttc 180
atggagtcga gttgcagact ccaatctgga ctacgatagg ctttttgaga ttcgcatcac 240
atcgctgtgt agctgccctc tgtacctacc attgtagcac gtgtgtagcc ctggtcgtaa 300
gggccatgat gacttgacgt cgtccccgcc ttcctccagt ttgtcactgg cagtatcctt 360
agagttcccg gcttaacccg ctggtaacta aggacagggg ttgcgctcgt tgcgggactt 420
aacccaacat ctcacgacac gagctgacga cagccatgca gcacctgtat tctaattccc 480
gaaggcactc ccgcatctct gcaggattct agatatgtca agaccaggta aggttcttcg 540
cgttgcatcg aattaaacca catgctccac cgcttgtgcg ggcccccgtc aattcatttg 600
agttttaacc ttgcggccgt actccccagg cggtctactt attgcgttag ctgcgtcact 660
aagtcctcaa gggacccaac gactagtaga catcgtttac ggcgtggact accagggtat 720
ctaatcctgt ttgctaccca cgctttcgaa cctcagtgtc aatatgatgc cagaaggctg 780
ccttcgccat cggtattcct ccagatctct acgcatttca ccgctacacc tggaattcta 840
ccttcctctc acctattcta gcctaacagt atcagatgca gttcccaggt taagcccggg 900
gatttcacat ctgacttatc aagccaccta cgctcgcttt acgcccagta attccgatta 960
acgcttgcac cctctgtatt accgcggctg ctggcacaga gttagccggt gcttattctg 1020
cagctaatgt catcgtccat gggtattaac catggagtct tcttcactgc ttaaagtgct 1080
ttacaaccaa aaggccttct tcacacacgc ggcatggctg gatcagggtt gcccccattg 1140
tccaatattc cccactgctg cctcccgtag gagtccgggc cgtgtctcag tcccggtgtg 1200
gctgatcatc ctctcagacc agctacagat cgtcgccatg gtaggccttt accccaccat 1260
ctagctaatc cgacttaggc tcatctaata gcgagagcaa caagttgccc cctttctccc 1320
gtaggtcgta tgcggtatta attcgagttt ccccgagcta tcccccacta ctaggtagat 1380
tcctaagtat tactcacccg tccgccgctc gacgcctgtt agcaagctat catcgtttcc 1440
gctcgacttg catgtgttaa gcctgccgcc agcgttcaat ctgagccagg atcaaactct 1500
Claims (5)
1. one plant of soil Psychrobacter, which is characterized in that the bacterial strain is soil Psychrobacter, and it is thermophilic that isolated strains are named as soil
Cold bacillus DL1901(Psychrobacter sp.SOD-DL1901), Chinese Typical Representative culture is preserved on June 6th, 2019
Object collection, the deposit date is on June 6th, 2019, deposit number was CCTCC NO:M 2019443.
2. application of the soil Psychrobacter described in claim 1 in the fermentation of agricultural solid residue low temperature aerobic composting.
3. one plant of soil Psychrobacter according to claim 1, which is characterized in that the screening mode of the bacterial strain are as follows: take
Sterile saline is added with the ratio of 100ml/10g in sample, and shaking table 150r/min, concussion, dilution spread is in primary dcreening operation culture
Base Low- temperature culture, picking grow bacterium colony switching pure culture.
4. one plant of soil Psychrobacter according to claim 3, which is characterized in that primary dcreening operation culture medium are as follows: dipotassium hydrogen phosphate
0.1%, cysteine 0.025%, yeast powder 0.2%, agar 2%, sodium acetate 0.25%, magnesium sulfate 0.01%, glucose 0.2%, soybean
Peptone 0.5%, is prepared with purified water, 7.2,37 DEG C of culture 24-48h of pH value.
5. one plant of soil Psychrobacter according to claim 3, which is characterized in that acquisition extremely frigid zones frozen soil layer air-dries
Sample is obtained with mortar grinder powdering after processing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910552737.7A CN110527643A (en) | 2019-06-25 | 2019-06-25 | One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910552737.7A CN110527643A (en) | 2019-06-25 | 2019-06-25 | One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110527643A true CN110527643A (en) | 2019-12-03 |
Family
ID=68659553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910552737.7A Pending CN110527643A (en) | 2019-06-25 | 2019-06-25 | One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110527643A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195136A (en) * | 2020-11-06 | 2021-01-08 | 呼伦贝尔学院 | Microbial agent for quickly warming compost at low temperature |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194408A (en) * | 2013-04-01 | 2013-07-10 | 广东省农业科学院土壤肥料研究所 | Psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and preparation method of psychrophilic microbial flora |
| CN107446866A (en) * | 2017-09-27 | 2017-12-08 | 福建省农科农业发展有限公司 | A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement |
| CN109665672A (en) * | 2019-01-25 | 2019-04-23 | 黑龙江大学 | A kind of device and Groundwater Treatment Methodss for strengthening total nitrogen in removal low temperature underground water |
-
2019
- 2019-06-25 CN CN201910552737.7A patent/CN110527643A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194408A (en) * | 2013-04-01 | 2013-07-10 | 广东省农业科学院土壤肥料研究所 | Psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and preparation method of psychrophilic microbial flora |
| CN107446866A (en) * | 2017-09-27 | 2017-12-08 | 福建省农科农业发展有限公司 | A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement |
| CN109665672A (en) * | 2019-01-25 | 2019-04-23 | 黑龙江大学 | A kind of device and Groundwater Treatment Methodss for strengthening total nitrogen in removal low temperature underground water |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195136A (en) * | 2020-11-06 | 2021-01-08 | 呼伦贝尔学院 | Microbial agent for quickly warming compost at low temperature |
| CN112195136B (en) * | 2020-11-06 | 2023-03-24 | 呼伦贝尔学院 | Microbial agent for quickly warming compost at low temperature |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101333510B (en) | Method for processing sludge and preparing bio organic fertilizer and special leaven thereof | |
| CN104560816B (en) | Bacillus licheniformis with biomass hydrolase activity and application thereof | |
| CN106701603A (en) | Preparation method of high-efficient decay-promoting agent | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN101709217B (en) | Pseudomonas fluorescens CKD18 and application thereof | |
| CN104894007A (en) | Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses | |
| CN105524858A (en) | High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum | |
| CN112522140A (en) | Microbial compound microbial inoculum for treating kitchen waste wheat straws and preparation method thereof | |
| Xie et al. | Study of biochemical and microbiological properties during co-composting of spent mushroom substrates and chicken feather | |
| CN111471621A (en) | Microbacterium MB338 and application thereof | |
| Wang et al. | Role of microbes and microbial dynamics during composting | |
| CN106399156A (en) | Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation | |
| CN104894025A (en) | Streptomyces sp. and application thereof | |
| CN103045500B (en) | Mesorhizobium KDRM295 and application thereof | |
| CN110527643A (en) | One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation | |
| CN114874953B (en) | Peanut rhizosphere biocontrol bacterium-Bacillus beleisi SW-1 and application thereof | |
| CN106222107A (en) | One strain is from the extreme thermophilic antibacterial of pig farm garbage | |
| CN110452859A (en) | One bacillus amyloliquefaciens B4216 and its application | |
| CN112624818B (en) | Excrement composting fermentation retting agent capable of killing insects and promoting growth and application thereof | |
| CN109609412A (en) | A kind of Thermophilic Bacteria Bacillus smithii Ths1 and its application | |
| CN108929162A (en) | Urban domestic garbage degradation and go removing heavy metals processing method | |
| CN109517756A (en) | One plant of ocean phosphorus decomposing bacillus megaterium and its application | |
| CN111172062B (en) | Sphingobacterium multivorum and application thereof | |
| CN107099486A (en) | Bacillus amyloliquefaciens GN03 and its application | |
| CN110484463A (en) | One plant of Mo Haiwei bacillus B282 and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200929 Address after: 116000 Xigou Village, Shuishiying Street, Lushunkou District, Dalian City, Liaoning Province Applicant after: DALIAN TAIFENGCHANG BIO-TECH Co.,Ltd. Address before: 116000 No.1, floor 2, No.85, longhejiayuan, Wuyi Road, Lvshunkou District, Dalian City, Liaoning Province Applicant before: Dalian taifengchang Agricultural Technology Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191203 |
|
| WD01 | Invention patent application deemed withdrawn after publication |