CN110522949B - Preparation method of compound brushite carrying native copper/rhizoma drynariae - Google Patents
Preparation method of compound brushite carrying native copper/rhizoma drynariae Download PDFInfo
- Publication number
- CN110522949B CN110522949B CN201911006486.9A CN201911006486A CN110522949B CN 110522949 B CN110522949 B CN 110522949B CN 201911006486 A CN201911006486 A CN 201911006486A CN 110522949 B CN110522949 B CN 110522949B
- Authority
- CN
- China
- Prior art keywords
- rhizoma drynariae
- powder
- microspheres
- brushite
- mixed powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Abstract
A method for preparing compound brushite carrying native copper/rhizoma drynariae comprises the following steps: weighing beta-calcium phosphate powder and monohydrate calcium phosphate powder according to a molar ratio of 1.1:1, grinding to obtain mixed powder A1, adding calcium alginate microspheres loaded with 3wt% of rhizoma drynariae extract, grinding to obtain mixed powder A2, weighing vinegar calcined pyrite and triple distilled water according to a ratio of 1:2, decocting with slow fire to a ratio of 11:1, and filtering to obtain filtrate B; adding the filtrate B into the mixed powder A2 according to the ratio of 2.5:1, stirring to obtain uniform paste, filling the paste into a mold, and taking out after solidification to obtain the composite material. Compared with medicines loaded with growth factors and the like, the composite microsphere has the advantages of lower price, simple and easily obtained raw materials, stable drug effect and suitability for large-scale production, and utilizes the characteristic that the synthetic environment of the brushite is acidic, and the calcium alginate microsphere does not swell under the acidic condition, so that the material system of the composite microsphere is more stable.
Description
Technical Field
The invention belongs to the field of biomedical materials, and particularly relates to a preparation method of a compound brushite carrying native copper/rhizoma drynariae.
Background
Pyritum has a long history in China as a bone-knitting wound-treating drug. According to the statistics of Chinese medicine prescription dictionary, in the 117 prescription of the ancient coronary fracture setting two characters, 43 people are used with native copper, and the number is up to 36.8%. The research considers that the native copper is ferrous mineral pyrite, contains rich iron, is beneficial to the formation of collagen in callus at the early stage of fracture healing, and also has the promotion effect on calcification of callus at the middle and late stages. Meanwhile, the native copper also contains various trace elements necessary for human bodies, such as copper, zinc, manganese and the like, so that the activity of various enzymes can be enhanced, the formation of collagen fibers and the deposition of calcium and phosphorus can be promoted, and the formation of callus can be promoted.
Rhizoma drynariae is another commonly used Chinese good bone-knitting medicine. Modern pharmaceutical research shows that the drynaria extract can promote the expression of bone growth factor, obviously enhance the proliferation of osteoblast, facilitate the calcification of bone and the formation of bone, increase the thickness of callus and improve the quality of fracture healing. Meanwhile, the experiments of acute toxicity of the total flavonoids of the drynaria rhizome show that the chains of the fetters in diet, activity and mental state have no abnormal change after the intragastric administration of mice and rats, and the fetters have good safety in clinical application and can be used for a long time. Rhizoma drynariae and native copper are used as traditional good bone-knitting medicines, and the rhizoma drynariae and the native copper are often used together, so that even if the traditional bone-knitting good bone-knitting medicines are decocted at high temperature, the medicine effect can still be kept stable.
The brushite is calcium hydrophosphate dihydrate, is a calcium phosphate bone repair material commonly used in clinic, has chemical components close to inorganic components of human bones, good biocompatibility and excellent degradation performance. Because the brushite is prepared by blending phosphate solid-phase powder and aqueous solution liquid phase during synthesis, the calcium phosphate salt is very suitable to be used as a loading system of the medicine to control the medicine release. In order to further improve the bone repair properties of brushite, various active biomolecules, such as bone morphogenetic proteins and insulin-like growth factors, are often loaded on brushite. However, most of these active biomolecules are very expensive, have short half-life, are easy to dissociate, have high requirements on the preparation process of the composite material, have short shelf life, increase the manufacturing cost of the material, and also increase the economic burden of patients in clinical use.
Disclosure of Invention
Aiming at the technical problems, the brushite with excellent bone repair performance is taken as a substrate, two good bone-knitting medicines commonly used in the traditional Chinese medicine orthopedics clinic, namely the native copper and the rhizoma drynariae are selected, and the two traditional Chinese medicines are compounded with the brushite according to the respective characteristics of the native copper, the rhizoma drynariae and the brushite to prepare the compound brushite carrying the native copper/the rhizoma drynariae.
The technical scheme of the invention is as follows: a method for preparing compound brushite carrying native copper/rhizoma drynariae comprises the following steps:
step one, weighing beta-calcium phosphate powder and calcium phosphate monohydrate powder according to the molar ratio of 1.1:1, putting the beta-calcium phosphate powder and the calcium phosphate monohydrate powder into a mortar, and grinding to obtain mixed powder A1;
step two, adding calcium alginate microspheres loaded with 3wt% of drynaria extract into the mixed powder A1, and grinding to obtain mixed powder A2;
step three, weighing vinegar calcined pyrite and triple-distilled water according to the ratio of 1:2, wherein the vinegar calcined pyrite is calculated by grams, the triple-distilled water is calculated by milliliters, boiling, decocting with slow fire to the ratio of 11:1, and filtering to obtain filtrate B;
and step four, adding the filtrate B into the mixed powder A2 according to the ratio of 2.5:1, wherein the mixed powder A2 is calculated by grams, the filtrate B is calculated by milliliters, stirring is carried out for 20 seconds by adopting a stainless steel knife to obtain uniform paste, the paste is filled into a mold, and the paste is taken out after being solidified, so that the composite material with the regular shape can be obtained.
Further optimization, the grinding time in the step one is 15min, and the grinding time in the step two is 5 min.
Further optimizing, the diameter of the calcium alginate microspheres in the second step is 300-.
And further optimizing, wherein the adding amount of the calcium alginate microspheres in the second step is 1 wt% of the total mass of the powder.
Further optimizing, in the second step, the specific preparation method of the calcium alginate microspheres comprises the following steps: grinding rhizoma Drynariae extract into powder, adding into 1.5-2.5wt% sodium alginate solution, stirring, extracting with syringe, and dripping 2-6 wt% CaCl2Forming microspheres in the solution, standing for crosslinking, washing the microspheres with deionized water, soaking the microspheres in a citric acid solution, washing again, pre-freezing and freezing.
Further preferably, the citric acid solution is a 0.5M citric acid solution containing 1 wt% of chitosan.
The invention has the beneficial effects that:
firstly, brushite with excellent bone repair performance is taken as a substrate, two good bone-knitting medicines commonly used in clinical department of orthopedics in traditional Chinese medicine, namely pyrite and rhizoma drynariae are selected, the two traditional Chinese medicines are compounded with the brushite, wherein the drynaria rhizome medicament is loaded in a microsphere mode, the drynaria rhizome extract is loaded on calcium alginate microspheres with the diameter of 300-800 mu m, wherein more than 95 percent is distributed at 800 mu m of 300-, after the degradation, pores with corresponding sizes are formed, and the bionic bone tissue with a porous anatomical structure of 300-; the native copper medicine is loaded by decocting into a liquid medicine with a specific concentration, calcium alginate microspheres loaded with rhizoma drynariae are compounded with two kinds of phosphates of beta-calcium phosphate and calcium dihydrogen phosphate to prepare a composite phosphate raw material loaded with rhizoma drynariae, the native copper is decocted into a liquid medicine with a proper concentration to be used as a curing liquid, the composite phosphate raw material is blended with the native copper liquid medicine, and the native copper/rhizoma drynariae compound brushite is prepared after the curing of the composite phosphate raw material and the native copper liquid medicine;
secondly, the native copper and the rhizoma drynariae which are used as traditional Chinese medicines are cheap, and the native copper and the rhizoma drynariae are compounded on the brushite, so that the price is lower than that of medicines loaded with growth factors and the like; the raw materials are simple and easy to obtain, the drug effect is stable, the composite microsphere is suitable for large-scale production, and the characteristic that the synthetic environment of the brushite is acidic, and the calcium alginate microsphere does not swell under the acidic condition is utilized, so that the material system of the composite microsphere is more stable.
Drawings
FIG. 1 is a sectional view of example 1
FIG. 2 is a sectional view of example 2
FIG. 3 is a sectional view of example 3
FIG. 4 is a cross-sectional view of a pure material without added microspheres
FIG. 5 is a graph showing the proliferation of MTT in examples 1 to 3.
Detailed Description
The specific implementation mode of the invention is as follows:
example 1
A method for preparing compound brushite carrying native copper/rhizoma drynariae comprises the following steps:
step one, weighing beta-calcium phosphate powder and calcium phosphate monohydrate powder which are sieved by a sieve of 200 meshes according to the molar ratio of 1.1:1, putting the beta-calcium phosphate powder and the calcium phosphate monohydrate powder into a mortar, and grinding for 15min to obtain mixed powder A1;
step two, preparing 1.5wt% sodium alginate solution and 2wt% CaCl2Mixing the solution with 0.5M citric acid solution containing 1 wt% chitosan, grinding rhizoma Drynariae extract into powder, adding into sodium alginate solution, stirring, extracting with syringe, and adding CaCl dropwise2Forming microspheres in the solution, standing and crosslinking for 4 hours, washing the microspheres with deionized water, soaking the microspheres in a citric acid solution, washing with deionized water again, pre-freezing for 24 hours at-80 ℃, freeze-drying for 6 hours by using a freeze dryer to obtain calcium alginate microspheres loaded with a drynaria extract of 3wt%, adding calcium alginate microspheres which account for 1 wt% of the total mass of the powder A1 into the powder A1, wherein the diameter of the calcium alginate microspheres is more than 95% and is distributed at 800 mu m of 300-;
step three, weighing vinegar calcined pyrite and triple-distilled water according to the ratio of 1:2, wherein the vinegar calcined pyrite is calculated by grams, the triple-distilled water is calculated by milliliters, boiling, decocting with slow fire until the ratio of the vinegar calcined pyrite to the triple-distilled water is 11:1, and filtering to obtain a filtrate B;
step four, adding the filtrate B into the mixed powder A2 according to the proportion of 2.5:1, wherein the mixed powder A2 is calculated by grams, the filtrate B is calculated by milliliters, then stirring for 20 seconds by using a stainless steel knife to obtain uniform paste, filling the paste into a mold, and taking out after the paste is solidified to obtain the composite material with a regular shape.
Example 2
A method for preparing compound brushite carrying native copper/rhizoma drynariae comprises the following steps:
step one, weighing beta-calcium phosphate powder and calcium phosphate monohydrate powder which are sieved by a sieve of 200 meshes according to the molar ratio of 1.1:1, putting the beta-calcium phosphate powder and the calcium phosphate monohydrate powder into a mortar, and grinding for 15min to obtain mixed powder A1;
step two, preparing 2wt% sodium alginate solution and 4wt% CaCl2Mixing the solution with 0.5M citric acid solution containing 1 wt% chitosan, grinding rhizoma Drynariae extract into powder, adding into sodium alginate solution, stirring, extracting with syringe, and adding CaCl dropwise2Forming microspheres in the solution, standing and crosslinking for 4 hours, washing the microspheres with deionized water, soaking the microspheres in a citric acid solution, washing with deionized water again, pre-freezing for 24 hours at-80 ℃, freeze-drying for 6 hours by using a freeze dryer to obtain calcium alginate microspheres loaded with a drynaria extract of 3wt%, adding calcium alginate microspheres which account for 1 wt% of the total mass of the powder A1 into the powder A1, wherein the diameter of the calcium alginate microspheres is more than 95% and is distributed at 800 mu m of 300-;
step three, weighing vinegar calcined pyrite and triple-distilled water according to the proportion of 1:2, wherein the vinegar calcined pyrite is calculated by grams, the triple-distilled water is calculated by milliliters, boiling, decocting with slow fire until the solid-to-liquid ratio is 11g:1ml, and filtering to obtain filtrate B;
step four, adding the filtrate B into the mixed powder A2 according to the proportion of 2.5:1, wherein the mixed powder A2 is calculated by grams, the filtrate B is calculated by milliliters, then stirring for 20 seconds by using a stainless steel knife to obtain uniform paste, filling the paste into a mold, and taking out after the paste is solidified to obtain the composite material with a regular shape.
Example 3
A method for preparing compound brushite carrying native copper/rhizoma drynariae comprises the following steps:
step one, weighing beta-calcium phosphate powder and calcium phosphate monohydrate powder which are sieved by a sieve of 200 meshes according to the molar ratio of 1.1:1, putting the beta-calcium phosphate powder and the calcium phosphate monohydrate powder into a mortar, and grinding for 15min to obtain mixed powder A1;
step two, preparing 2.5wt% sodium alginate solution and 6 wt% CaCl2Mixing the solution with 0.5M citric acid solution containing 1 wt% chitosan, grinding rhizoma Drynariae extract into powder, adding into sodium alginate solution, stirring, extracting with syringe, and adding CaCl dropwise2Forming microspheres in the solution, standing and crosslinking for 4 hours, washing the microspheres with deionized water, soaking the microspheres in a citric acid solution, washing with deionized water again, pre-freezing for 24 hours at-80 ℃, freeze-drying for 6 hours by using a freeze dryer to obtain calcium alginate microspheres loaded with a drynaria extract of 3wt%, adding calcium alginate microspheres which account for 1 wt% of the total mass of the powder A1 into the powder A1, wherein the diameter of the calcium alginate microspheres is more than 95% and is distributed at 800 mu m of 300-;
step three, weighing vinegar calcined pyrite and triple-distilled water according to the proportion of 1:2, wherein the vinegar calcined pyrite is calculated by grams, the triple-distilled water is calculated by milliliters, boiling, decocting with slow fire until the solid-to-liquid ratio is 11g:1ml, and filtering to obtain filtrate B;
step four, adding the filtrate B into the mixed powder A2 according to the proportion of 2.5:1, wherein the mixed powder A2 is calculated by grams, the filtrate B is calculated by milliliters, then stirring for 20 seconds by using a stainless steel knife to obtain uniform paste, filling the paste into a mold, and taking out after the paste is solidified to obtain the composite material with a regular shape.
Carrying out morphology detection and cytotoxicity detection on the drug-loaded microspheres prepared in examples 1-3, wherein the morphology detection is carried out by taking a picture by using a digital camera and observing the section morphology of the material, and fig. 1 is a section of example 1, which shows that the microspheres are dispersed and embedded into a brushite matrix, and simultaneously, holes left by the occupied microspheres can also be observed due to separation and stripping; FIG. 2 is a cross-section of example 2 showing a plurality of microspheres dispersed embedded in a brushite matrix, with voids left by the microspheres being observed as well due to delamination; FIG. 3 is a cross-section of example 3 showing a plurality of microspheres dispersed embedded in a brushite matrix, with voids left by the microspheres being observed as well as being occupied by the microspheres due to the separation of the exfoliation, FIG. 4 is a blank control without microspheres added, showing material densification; cytotoxicity detection the MTT method is detected by adopting the cytotoxicity in the national standard GB/T16886.5-2017, the leaching liquor of the material is used for detecting the cytotoxicity, the cells are human osteoblast MG63, the initial inoculation density of the cells is 3000 cells/hole in a 96-well plate, the detection result is shown in figure 5, and the absorbance value OD of the samples 1-3 is higher than that of a blank control group without microspheres, which shows that the material has no cytotoxicity and simultaneously shows a certain stimulation effect on the growth of the cells.
The foregoing illustrates and describes the principal features, utilities, and principles of the invention, as well as advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made in the invention without departing from the spirit and scope of the invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A preparation method of a compound brushite bone repair material carrying native copper/rhizoma drynariae is characterized by comprising the following steps:
step one, weighing beta-calcium phosphate powder and Ca (H) according to the molar ratio of 1.1:12PO4 )2·H2Putting the O powder into a mortar, and grinding to obtain mixed powder A1;
step two, adding calcium alginate microspheres loaded with 3wt% of drynaria extract into the mixed powder A1, and grinding to obtain mixed powder A2;
step three, weighing vinegar calcined pyrite and triple-distilled water according to the ratio of 1:2, wherein the vinegar calcined pyrite is calculated by grams, the triple-distilled water is calculated by milliliters, boiling, decocting with slow fire to the ratio of 11:1, and filtering to obtain filtrate B;
and step four, adding the filtrate B into the mixed powder A2 according to the ratio of 2.5:1, wherein the mixed powder A2 is calculated by grams, the filtrate B is calculated by milliliters, stirring is carried out for 20 seconds by adopting a stainless steel knife to obtain uniform paste, the paste is filled into a mold, and the paste is taken out after being solidified, so that the composite material with the regular shape can be obtained.
2. The method for preparing the compound brushite bone repair material carrying the native copper/the rhizoma drynariae according to claim 1, wherein the grinding time in the step one is 15min, and the grinding time in the step two is 5 min.
3. The method for preparing the compound brushite bone repair material carrying the native copper/the rhizoma drynariae as claimed in claim 1, wherein the diameter of the calcium alginate microspheres in the second step is 300-800 μm.
4. The method for preparing the compound brushite bone repairing material carried with native copper/rhizoma drynariae according to claim 1, wherein the adding amount of the calcium alginate microspheres in the second step is 1 wt% of the total mass of the powder.
5. The method for preparing the composite brushite bone repair material carrying the native copper/the rhizoma drynariae according to claim 1, wherein in the second step, the specific preparation method of the calcium alginate microspheres comprises the following steps: grinding rhizoma Drynariae extract into powder, adding into 1.5-2.5wt% sodium alginate solution, stirring, extracting with syringe, and dripping 2-6 wt% CaCl2Forming microspheres in the solution, standing for crosslinking, washing the microspheres with deionized water, soaking the microspheres in a citric acid solution, washing again, pre-freezing and freezing.
6. The method for preparing the natural copper/rhizoma drynariae loaded compound brushite bone repair material according to claim 5, wherein the citric acid solution is 0.5M citric acid solution containing 1 wt% chitosan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911006486.9A CN110522949B (en) | 2019-10-22 | 2019-10-22 | Preparation method of compound brushite carrying native copper/rhizoma drynariae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911006486.9A CN110522949B (en) | 2019-10-22 | 2019-10-22 | Preparation method of compound brushite carrying native copper/rhizoma drynariae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110522949A CN110522949A (en) | 2019-12-03 |
| CN110522949B true CN110522949B (en) | 2021-11-09 |
Family
ID=68672110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911006486.9A Active CN110522949B (en) | 2019-10-22 | 2019-10-22 | Preparation method of compound brushite carrying native copper/rhizoma drynariae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110522949B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114344563B (en) * | 2022-01-28 | 2023-04-18 | 河南科技大学 | Material with antibacterial and bone-promoting properties and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004049589A (en) * | 2002-07-22 | 2004-02-19 | Toshiba Ceramics Co Ltd | Irregularly shaped bone filler material |
| CN1605362A (en) * | 2003-10-08 | 2005-04-13 | 西南交通大学 | Compound calcium phosphate/Chinese medicine bone alternate material and its preparing method |
| CN102114271A (en) * | 2011-02-24 | 2011-07-06 | 西南交通大学 | Multi-medicament loaded calcium phosphate cement powder |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1052651C (en) * | 1996-09-17 | 2000-05-24 | 姚永全 | Gujinkang traditional Chinese medicine and preparation method |
| CN1278744C (en) * | 2005-06-08 | 2006-10-11 | 天津大学 | Macroporous brushite bone cement with latent hole forming agent and preparation process thereof |
| CN100531805C (en) * | 2006-11-29 | 2009-08-26 | 华南理工大学 | Injectable self-cured calcium phosphate bone-rehabilitating material and its preparing process |
| CN104174342B (en) * | 2014-08-14 | 2016-04-13 | 暨南大学 | A kind of bata-tricalcium phosphate/alginic acid complex microsphere and method for making thereof and application |
| CN104666250B (en) * | 2015-03-06 | 2017-09-22 | 华南农业大学 | A kind of preparation method of aureomycin sustained-release micro-spheres |
| CN107802884B (en) * | 2017-12-07 | 2021-02-23 | 南京中医药大学 | 3D printing support used as bone repair biological material and preparation method thereof |
-
2019
- 2019-10-22 CN CN201911006486.9A patent/CN110522949B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004049589A (en) * | 2002-07-22 | 2004-02-19 | Toshiba Ceramics Co Ltd | Irregularly shaped bone filler material |
| CN1605362A (en) * | 2003-10-08 | 2005-04-13 | 西南交通大学 | Compound calcium phosphate/Chinese medicine bone alternate material and its preparing method |
| CN102114271A (en) * | 2011-02-24 | 2011-07-06 | 西南交通大学 | Multi-medicament loaded calcium phosphate cement powder |
Non-Patent Citations (1)
| Title |
|---|
| Zi-Ran-Tong loaded brushite bone cement with enhanced osteoblast mineralization ability in vitro;Zhengjun Pei等;《Materials Letters》;20191030;第259卷;第126908.1-126908.4页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110522949A (en) | 2019-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103272279B (en) | Bioactive multilayer multiphase ceramic microsphere material and its preparation method and use | |
| CN100425296C (en) | Collagen base bionic bone matrix | |
| CN102526798A (en) | Injectable compound bone cement and preparation method thereof | |
| CN108273131A (en) | A kind of composite bone cement, preparation method and applications and a kind of bone renovating material | |
| CN105251058B (en) | A kind of preparation method for carrying medicine and promoting bone uptake injection-type calcium sulfate bone cement | |
| CN110522949B (en) | Preparation method of compound brushite carrying native copper/rhizoma drynariae | |
| CN110237301B (en) | Sodium alginate-based inducible bone repair gel and preparation method and application thereof | |
| CN108836950A (en) | A kind of albumen sporopollenin takes orally the preparation method of microcapsules | |
| CN110075351A (en) | A kind of double drug release PMMA composite bone cements and preparation method thereof | |
| CN101297853B (en) | Bone-melon extract injection and preparation thereof | |
| CN106924196A (en) | A kind of growth factor-loaded slow-release gelatin microspheres and preparation method thereof | |
| RU2005127040A (en) | CERAMIC-INJECTED IMPLANTS FOR THE FILLING OF WRINKLES, SKIN DENTALS AND SCARS, AND THE METHOD FOR PRODUCING THEM | |
| CN109331223B (en) | Medicine-carrying bioactive glass composite calcium phosphate bone cement and application thereof | |
| CN109010922A (en) | Bone Defect Repari nano fibrous membrane and preparation method thereof | |
| CN113349379A (en) | Composition for improving bone health and preparation method thereof | |
| CN105412986A (en) | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof | |
| CN105327395B (en) | A kind of preparation method of acid hydroxy group apatite catalytic curing bone cement | |
| CN108159021A (en) | A kind of two-layer compound sustained-release micro-spheres for being loaded with promoting bone growing albumen and eliminating arthritic symptom drug and preparation method thereof | |
| CN105536059A (en) | Self-repairing injectable bone cement and preparation method | |
| CN104382033A (en) | Compound protein tabletted candy and preparation method thereof | |
| KR101727031B1 (en) | Health functional composition extracted from antler for preventing or treating the osteoporosis | |
| CN103007341A (en) | Bone cement modified by sulfate and preparation method of bone cement | |
| CN102166371B (en) | Medicine controlled timing-released calcium phosphate cement powder containing various medicines | |
| CN105770098A (en) | Blood pressure reducing composition and application thereof | |
| CN106806403B (en) | Traditional Chinese medicine compound composition for increasing bone mineral density and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |