CN110522834A - It blooms day lily extract and preparation method thereof, purposes and medicine composition for treating depression - Google Patents
It blooms day lily extract and preparation method thereof, purposes and medicine composition for treating depression Download PDFInfo
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Abstract
The invention belongs to natural medicine technical fields, and in particular to bloom day lily extract and preparation method thereof, purposes and medicine composition for treating depression.The invention discloses bloom day lily method for preparing extractive and its products of simple economy environmental protection, and disclose application of the day lily extract in preparation antidepressant of blooming, and including the medicine composition for treating depression that day lily extract is active material of blooming, such use is confirmed by pharmacological evaluation.The present invention greatly develops the application value for the day lily that blooms, and day lily extract of blooming is used for the first time and takes enteric microorganism metabolite hypothesis, the day lily extract that shows to bloom can significantly affect mouse intestinal microorganism to treat depression, with good application prospect.
Description
Technical field
The invention belongs to natural medicine technical fields, and in particular to bloom day lily extract and preparation method thereof, purposes
And medicine composition for treating depression.
Background technique
Day lily also known as " day lily ", " forgetting sorrow grass ", " tawny daylily ".Belong to Liliaceae day lily, there is nutrition abundant
Composition.The edible part of day lily will generate very big fiber sense, seriously once day lily Post flowering is eaten again for bud
Eating mouth feel is influenced, offal treatment will be usually treated as.Day lily is the very characteristic traditional vegetables in China, it is low in calories but
Great nutritive value.Day lily is rich in nutriment, wherein having: carbohydrate, protein, lipid, vitamin and minerals.Chinese medicine
Traditional medicine think that the function of day lily has: diminish inflammation resist bacterium, eliminate swelling and pain, diuresis, calming the liver blood-nourishing, stop blooding town
Bitterly, stomach invigorating and tranquilizing the mind etc., can treat depression, yellow subcutaneous ulcer, hepatitis, painful swelling of joints, urinary tract bacterium infection, fecal blood, dysentery,
The various diseases such as flu.
In day lily other than flavonoids, Anthraquinones, dihydrofuran-gamma-lactam class compound report are in the majority, also wrap
It includes: the classes such as alkaloid, terpene, phenolic acid class, sterol；In addition, scientist is also found: saponin, aliphatic, single phenyl ring derivative etc.
Compound.These above-mentioned compounds are studied to prove there is antidepression, anticancer, anti-oxidant, antibacterial, desinsection isoreactivity.Application
Number for CN 101327289 patent of invention in disclose day lily and its general flavone preparation antidepressant in application, tool
Body discloses serotonin, the norepinephrine, dopamine, 5- hydroxyl of day lily and its general flavone in preparation raising Cerebral cortex
Application in base heteroauxin drug, and disclose day lily and its general flavone and preparing the application in antagonism reserpine drug；
Above-mentioned application is confirmed by pharmacological evaluation.But the patent is to show day lily by detecting the variation of the mouse Nerve mediator factor
There is antidepressant effect, it does not disclose that using the day lily of which kind of character, and is heated using 60%-90% ethyl alcohol
Then refluxing extraction is extracted with water to obtain day lily antidepression total flavone part, process is relatively complicated, and extraction cost is higher.
Summary of the invention
Against the above technical problems, the object of the present invention is to provide a kind of easy to operate, lower Huangs of blooming of extraction cost
The preparation method of cauliflower extract, bloom day lily extract and application thereof and a kind of medicine composition for treating depression of preparation.
To achieve the goals above, the present invention adopts the following technical scheme:
Firstly, the present invention provides the preparation method for day lily extract of blooming.
The day lily extract of blooming is bloom day lily water extract or day lily alcohol extract of blooming.
The preparation method of the day lily water extract of blooming the following steps are included:
(1) day lily that will bloom grinds, and the normal-temperature water that 6-10 times of quality is added extracts 5-7h, concentration extraction after having extracted
Liquid obtains the first medicinal extract；
(2) normal-temperature water that 6-10 times of quality is continuously added in residue extracts 2-4h, has extracted concentrated extracting solution afterwards, has obtained the
Two medicinal extract；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, 24- will be freezed under the conditions of -80 DEG C of third medicinal extract
36h；
(4) it after the third medicinal extract freezed being freeze-dried 72-96h, grinds and beats powder, day lily water extract of blooming is made.
Preferably, the day lily water extract of blooming preparation method the following steps are included:
(1) day lily that will bloom grinds, and the normal-temperature water that 8 times of quality are added extracts 6h, has extracted concentrated extracting solution afterwards, has obtained the
One medicinal extract；
(2) normal-temperature water that 8 times of quality are continuously added in residue extracts 3h, and concentrated extracting solution after having extracted obtains the second leaching
Cream；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, will be freezed for 24 hours under the conditions of -80 DEG C of third medicinal extract；
(4) it is taken after the third medicinal extract freezed being freeze-dried 72h, grinds and beat powder, day lily water extract of blooming is made.
The preparation method of the day lily alcohol extract of blooming the following steps are included:
(1) day lily that will bloom grinds, and the ethyl alcohol that the volumetric concentration that 6-10 times of quality is added is 80-90% extracts 5-7h,
Concentrated extracting solution after having extracted, obtains the first medicinal extract；
(2) ethyl alcohol that the volumetric concentration that 6-10 times of quality is continuously added in residue is 80-90% extracts 2-4h, has extracted
Concentrated extracting solution afterwards obtains the second medicinal extract；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, 24- will be freezed under the conditions of -80 DEG C of third medicinal extract
36h；
(4) it after the third medicinal extract freezed being freeze-dried 72-96h, grinds and beats powder, day lily alcohol extract of blooming is made.
Preferably, the day lily alcohol extract of blooming preparation method the following steps are included:
(1) day lily that will bloom grinds, and the ethyl alcohol that the volumetric concentration that 8 times of quality are added is 85% extracts 6h, after having extracted
Concentrated extracting solution obtains the first medicinal extract；
(2) ethyl alcohol that the volumetric concentration that 8 times of quality are continuously added in residue is 85% extracts 3h, and concentration mentions after having extracted
Liquid is taken, the second medicinal extract is obtained；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, will be freezed for 24 hours under the conditions of -80 DEG C of third medicinal extract；
(4) it after the third medicinal extract freezed being freeze-dried 72h, grinds and beats powder, day lily alcohol extract of blooming is made.
Secondly, the present invention provides day lily extract prepared by the preparation method of blooming.
Again, the present invention provides purposes of the day lily extract in preparation antidepressant of blooming.
Finally, the present invention also provides include above-mentioned antidepressant combination of the day lily extract as active constituent of blooming
Object.
The antidepressant is to reduce Firmicutes micro organism quantity in enteron aisle, improve Bacteroidetes microorganism in enteron aisle
Quantity reduces Desulfovibrio category and Unidentified Lachnospiraceae microorganism belonging to genus quantity in enteron aisle, improves
Alloprevotella belongs to the one of which with the drug of Bacteroides microorganism belonging to genus quantity in enteron aisle.
Pharmaceutically acceptable any conventional can be made according to conventional preparation process in the medicine composition for treating depression
The preparation formulations such as dosage form, such as capsule, tablet, granule, powder, oral solution, pill.
To can be realized above-mentioned dosage form, pharmaceutically acceptable auxiliary material need to be added when preparing these dosage forms, such as: it fills out
Fill agent, disintegrating agent, lubricant, suspending agent, adhesive, sweetener, corrigent, preservative, matrix etc..
Filler includes starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose etc. wherein one
Kind is a variety of.Disintegrating agent includes starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, crosslinked polyethylene pyrrolidines
The one or more of them such as ketone, low-substituted hydroxypropyl cellulose, croscarmellose sodium.Lubricant includes magnesium stearate, ten
The one or more of them such as sodium dialkyl sulfate, talcum powder, silica.Suspending agent includes polyvinylpyrrolidone, crystallite fibre
Tie up the one or more of them such as element, sucrose, agar, hydroxypropyl methyl cellulose.Adhesive includes starch slurry, polyvinylpyrrolidine
The one or more of them such as ketone, hydroxypropyl methyl cellulose.Sweetener includes saccharin sodium, aspartame, sucrose, honey element, sweet
The one or more of them such as careless hypo acid.Corrigent includes sweetener, natural perfume material, artificial flavors, starch, carboxymethyl cellulose
The one or more of them such as sodium, bicarbonate, organic acid.Preservative includes parabens, benzoic acid, sodium benzoate, sorbic acid
And the one or more of them such as its esters, benzalkonium bromide, acetic acid chloroethene are fixed, eucalyptus oil.Matrix include PEG6000, PEG4000,
The one or more of them such as insect wax.
Compared with prior art, the invention has the following beneficial effects:
(1) present invention extracts its effective efficiency ingredient with normal-temperature water or ethyl alcohol using day lily is bloomed as raw material,
The day lily that makes to bloom is turned waste into wealth, and resource is made full use of；Day lily will be bloomed as raw material and carry out antidepressant activity screening, pole
The great development application value for the day lily that blooms, directlys adopt normal-temperature water or ethyl alcohol extracts, and step is simple, economic and practical,
Any pollution will not be generated.
(2) first demonstration that day lily extract of blooming can significantly improve in mouse intestinal with antidepressant effect
Bacteroidetes microorganism, beneficial bacteria Alloprevotella belong to and Bacteroides microorganism belonging to genus quantity, reduce heavy wall
Bacterium door microorganism, harmful bacteria Desulfovibrio belongs to and the number of Unidentified Lachnospiraceae microorganism belonging to genus
Amount, there were significant differences compared with model group.
After administration 35 days, compared with normal group, the syrup preference index of model group mouse is substantially reduced (P < 0.05)；With
Model group is compared, and day lily water extract of blooming is low, high dose group mouse syrup preference index is significantly raised (P < 0.01), Huang of blooming
Cauliflower alcohol extract is low, high dose group mouse syrup preference index has certain raising；Fluoxetine hydrochloride group mouse syrup has a preference for index
Significantly raised (P < 0.05).
Compared with normal group, the incubation period of ingesting of model group mouse is obviously prolonged (P < 0.01)；Compared with model group, open
Flower day lily water extract is low, high dose group mouse ingests, and incubation period is obviously shortened (P < 0.05)；It blooms the high agent of day lily alcohol extract
Amount group mouse incubation period of ingesting is obviously shortened (P < 0.01)；Fluoxetine hydrochloride group mouse ingest incubation period be obviously shortened (P <
0.01)。
Compared with normal group, the activity time significantly reduces (P < 0.05) in the test of model group mouse tail suspension, and quiescent time is bright
It is aobvious to extend (P < 0.01)；Compared with model group, day lily water extract of blooming is low, the high dose group mouse activity time is obviously prolonged (P
< 0.05), quiescent time significantly reduces (P < 0.05)；Day lily alcohol extract of blooming is low, the high dose group mouse activity time obviously prolongs
Long (P < 0.05 or P < 0.01), quiescent time significantly reduce (P < 0.05 or P < 0.01)；The Fluoxetine hydrochloride group mouse activity time
It is obviously prolonged (P < 0.05), quiescent time significantly reduces (P < 0.05).
With not blooming, day lily water is mentioned, compared with alcohol extract, bloom day lily water extract and the chrysanthemum that blooms prepared by the present invention
Dish alcohol extract antidepressant effect is obviously more excellent, and day lily water extract effective dose of blooming is lower than day lily alcohol extract of blooming, table
It is bright to bloom day lily extract with good application prospect.
Detailed description of the invention
Fig. 1 is the relative abundance of mouse intestinal microbiologic population under different disposal door level.
Fig. 2 is Firmicutes relative abundance under different disposal.
Fig. 3 is Bacteroidetes relative abundance under different disposal.
Fig. 4 is bloom day lily water extract, day lily alcohol extract of blooming, do not bloom day lily water extract and the chrysanthemum that do not bloom
The total ion current figure of dish alcohol extract.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the embodiment of the present invention, to this hair
Technical solution in bright embodiment is clearly and completely described, it is clear that described embodiment is that a part of the invention is implemented
Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creativeness
Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of labour.
Day lily kind used in following embodiment is sudden dive flower.Day lily extract, lot number: 20180720, by lake
Southern agriculture university provides.Fluoxetine hydrochloride dispersible tablet, lot number: the Suzhou 16090487345A, Li Lai Co., Ltd product.Experiment is dynamic
Object is male ICR mouse 140, weight 18.0-22.0g, animal quality quality certification number: 43004700048590, it is purchased from Hunan
This Lake scape reaches experimental animal Co., Ltd, experimental animal production licence number: SCXK (Hunan) 2011-0003；In Hunan Province's medicine
The raising of object Research on Safety Assessment center barrier environment, experimental animal use credit number: SYXK (Hunan) 2015-0016.0.9% chlorine
Change sodium injection (lot number: 20170112, Hunan Kangyuan Pharmacy Co., Ltd.'s product).Toy hangs tail tester, Chengdu Tai Meng
Science and technology Co., Ltd product.Other chemical reagent used can be commercially available from usual manner.
Embodiment 1 prepares day lily water extract of blooming
(1) day lily that will bloom grinds, and the normal-temperature water that 8 times of quality are added extracts 6h, has extracted concentrated extracting solution afterwards, has obtained the
One medicinal extract；
(2) normal-temperature water that 8 times of quality are continuously added in residue extracts 3h, and concentrated extracting solution after having extracted obtains the second leaching
Cream；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, will be freezed for 24 hours under the conditions of -80 DEG C of third medicinal extract；
(4) it is taken after the third medicinal extract freezed being freeze-dried 72h, grinds and beat powder, day lily water extract of blooming is made.
Embodiment 2 prepares day lily alcohol extract of blooming
(1) day lily that will bloom grinds, and the ethyl alcohol that the volumetric concentration that 8 times of quality are added is 85% extracts 6h, after having extracted
Concentrated extracting solution obtains the first medicinal extract；
(2) ethyl alcohol that the volumetric concentration that 8 times of quality are continuously added in residue is 85% extracts 3h, and concentration mentions after having extracted
Liquid is taken, the second medicinal extract is obtained；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, will be freezed for 24 hours under the conditions of -80 DEG C of third medicinal extract；
(4) it after the third medicinal extract freezed being freeze-dried 72h, grinds and beats powder, day lily alcohol extract of blooming is made.
Fig. 4 is the total ion current figure of bloom the day lily water extract and day lily alcohol extract of blooming that are prepared, You Tuke
Know two kinds of extracts at being grouped as certain difference.
3 day lily extract of embodiment stress cause the influence of depression model mice to chronic unpredictability
(1) the chronic unpredictable foundation that stress cause depression model mice
Male ICR mouse 140, weight 18.0-22.0g, in addition to Normal group 10, remaining mouse is carried out chronic
It is unpredictable stress, including fasting (12h), prohibit water (12h), forced swimming (10min), stroboscopic (12h), noise (30min), beam
(12h) is tied up (to be placed in 50ml centrifuge tube, pipe diameter 3.0cm, be about 10cm, it is the ventilation of 0.5mm that tube wall, which has 6-7 diameter,
Mouthful), the cage that inclines (12h), wet cage (12h), round the clock overturn etc., animal is fasting for solids and liquids during constraint.
The chronic unpredictability stimulation time table of table 1
(2) grouping and administration
Weight is taken into account according to syrup preference testing result after the continuous modeling of animal 28 days to be grouped animal at random, is respectively as follows:
Normal group, model control group, Fluoxetine hydrochloride group, day lily water extract of blooming be low, high dose group, Normal group and mould
Distilled water is given in the daily stomach-filling of type control group, remaining each group gives corresponding medical fluid respectively, and administered volume 20mL/kg continuously gives
Medicine 35 days, after administration mouse continue chronic unpredictability stress, respectively at after the last administration to mouse carry out syrup it is inclined
Love detection, novel environment inhibit to ingest and hang the detection of tail behaviouristics.
(3) dosage is arranged
It is arranged that day lily is low, high dose 200mg/kg, 500mg/kg.Fluoxetine hydrochloride quantity 20mg, according to dynamic
Object and people's clinical dosage are converted into mouse dose are as follows: 20mg*0.0026/0.02kg=2.6mg/kg, using clinical equivalent dosage 2
Times, i.e. 5.2mg/kg is as mouse dosage.See Table 2 for details.
The test of table 2 grouping and dose design
(4) statistical method
For statistical analysis using SPSS16.0, the level set of statistical significance is P≤0.05, and measurement data is using equal
Number ± standard deviationIndicate, examine normality and homogeneity of variance with Leven ' s test method, if meet normality and
Homogeneity of variance (P > 0.05), it is for statistical analysis with one-way analysis of variance (ANOVA) and LSD test；If not meeting normal state
Property and homogeneity of variance (P≤0.05), then examined with Kruskal-Wallis, if Kruskal-Wallis inspection have statistics meaning
Adopted (P≤0.05), then be compared analysis with Dunnett ' s Test (nonparametric technique).When evaluation consider statistical difference and
Biological significance.
(5) Testing index
1. syrup preference test
Mouse carries out syrup preference detection, and syrup preference test is divided into training period and test phase.1st for 24 hours as training
Phase makes animal sufficiently adapt to sucrose drinking-water, gives animal two bottle of 1% sucrose water.2nd is also for 24 hours training period, gives mouse 1
1% sucrose water of bottle, 1 bottle of pure water.Non-fasting before testing prohibits water 8h.The test phase, while giving mouse 1 bottle of 1% sucrose water, 1 bottle is pure
Water tests animal in 15h and drinks syrup and pure water water respectively.Test is intermediate, by two bottles of location swaps to avoid Place Preference
Influence factor.Test terminates, and calculates syrup preference index (syrup preference index=syrup consumption/always liquid-consumed × 100%).
2. novel environment inhibition is ingested
Novelty is carried out after syrup preference detection and inhibits detection of ingesting, and experiment is always divided into 2 days, and the 1st day is the laundering period, by animal
It is put into rectangular spacious case and adapts to 10min.Novel inhibition feeding experiment detection is carried out afterwards for 24 hours in animal fasting (can't help water), in spacious case
A food ball is put at center, and animal back is put into food ball, keeps each same position, same direction, observes and records animal and be put into certainly
To feeding time (ingesting incubation period) for the first time in cage, each 5min, starting bite food ball with animal is standard of ingesting.
3. outstanding tail
The inhibition of novel environment ingests and carries out outstanding tail test to mouse after detection.Mouse is inverted, outstanding tail dress is fixed in tail portion
It sets, head makes mouse all around without the place grabbed is climbed, have baffle to separate sight between mouse, to prevent away from desktop about 5cm
It interferes with each other, detects the activity condition of every animal 4min.
(6) experimental result
1. influence of the day lily extract to depression model mouse syrup preference index
As shown in table 3, compared with Normal group, model control group mouse syrup preference index be substantially reduced (P <
0.05)；Compared with model control group, day lily water of not blooming mention low dosage mouse syrup preference index it is significantly raised (P <
0.01)；Day lily water of blooming proposes low, high dose group mouse syrup preference index apparent increase (P < 0.01)；Fluoxetine hydrochloride group
It is significantly raised (P < 0.05) that mouse syrup has a preference for index；Compared with proposing group with day lily water of blooming, do not bloom alcohol extracting high dose mouse
Syrup preference index is substantially reduced (P < 0.05).
The influence that 3 day lily extract of table has a preference for depressed mouse syrup
Note: compared with Normal group+P < 0.05,++P < 0.01, * P < 0.05 compared with model control group, P < 0.01 * *,
Compared with proposing group with water of not blooming#P < 0.05,##P<0.01。
2. day lily extract inhibits the influence ingested to depressed mouse novelty environment
As shown in table 4, compared with Normal group, model control group mouse incubation period of ingesting is obviously prolonged (P < 0.01)；
Compared with model is to group, day lily water of not blooming improves dosage mouse incubation period of ingesting and significantly reduces (P < 0.05)；Day lily opens
Hua Shui mentions low, high dose group mouse incubation period of ingesting and significantly reduces (P < 0.05)；Day lily does not bloom alcohol extracting high dose group mouse
Incubation period of ingesting significantly reduces (P < 0.05)；Day lily bloom alcohol extracting high dose group mouse ingest incubation period significantly reduce (P <
0.01)；Fluoxetine hydrochloride group mouse incubation period of ingesting significantly reduces (P < 0.01).
4 day lily extract of table ingests preclinical influence on depressed mouse
Note: compared with Normal group+P < 0.05,++P < 0.01, * P < 0.05 compared with model control group, P < 0.01 * *.
3. the influence that day lily extract tests depressed mouse tail suspension
As shown in table 5, compared with Normal group, the model control group mouse activity time is significantly reduced (P < 0.05), quiet
Only the time is obviously prolonged (P < 0.01)；Compared with model is to group, day lily water of not blooming mentions the low dosage activity time and is obviously prolonged
(P < 0.05) quiescent time significantly reduces (P < 0.05), day lily bloom water mention low, the high dose group activity time be obviously prolonged (P <
0.05), quiescent time significantly reduces (P < 0.05)；Day lily alcohol extracting high dose group mouse activity time of not blooming is obviously prolonged (P
< 0.05), quiescent time significantly reduces (P < 0.05).Day lily blooms, and alcohol extracting is low, the high dose group mouse activity time is obviously prolonged
(P < 0.05 or P < 0.01), quiescent time significantly reduce (P < 0.05 or P < 0.01)；The Fluoxetine hydrochloride group mouse activity time is bright
Aobvious to extend (P < 0.05), quiescent time significantly reduces (P < 0.05).
Influence of the 5 day lily extract of table to depressed mouse tail suspension
Note: compared with Normal group+P < 0.05,++P < 0.01, * P < 0.05 compared with model control group, P < 0.01 * *.
By the above analysis of experimental data it is found that syrup preference behaviouristics as the result is shown: day lily water of not blooming mention low dosage,
Water of blooming mentions low, high dose can significantly raised mouse syrup preference index.Novelty inhibit feeding experiment the result shows that: Huang of not blooming
Cauliflower water improve dosage, water of blooming mention low, high dose, day lily do not bloom alcohol extracting high dose, alcohol extracting high dose of blooming can be obvious
Mouse is reduced to ingest incubation period.Tail-suspention test the result shows that: when water of not blooming mentions low dosage and can be obviously prolonged mouse tail suspension activity
Between；Day lily water of blooming mentions that low, high dose, that alcohol extracting high dose of not blooming, alcohol extracting of blooming are low, high dose can be obviously prolonged mouse is outstanding
The tail activity time reduces quiescent time.
In conclusion not blooming day lily and blooms that water mentions, alcohol extract has certain antidepressant effect, wherein Huang of blooming
Cauliflower water mentions, alcohol extract antidepressant effect be substantially better than day lily do not bloom water mention, alcohol extracting extract, and day lily water of blooming mentions
Object effective dose is significantly lower than alcohol extracting position object, therefore day lily water extract antidepressant effect of blooming is obviously more excellent, has preferable
Application prospect.
Embodiment 4 blooms day lily extract to mouse intestinal microbiological effect
Experimental setup:
NC: MC is normally organized: model group FH: positive control drug Prozac group
HCW: day lily water of blooming mentions a group HCE: day lily alcohol extracting group of blooming
1. the influence horizontal to mouse intestinal microorganism door
Detect different disposal door level under mouse intestinal microbiologic population relative abundance, as a result as shown in Figure 1,
Firmicutes (Firmicutes) and the variation of Bacteroidetes (Bacteroidetes) quantity are more significant.
As shown in Fig. 2, model group (MC) Firmicutes number is obviously than normal group after mouse carries out chronic constriction depression
(NC) increase very much (P < 0.01), when use positive control drug Prozac (FH), day lily water of blooming mention (HCW) and the chrysanthemum that blooms
After dish alcohol extracting (HCE) sample, Firmicutes number is declined, but day lily water of blooming mention group (HCW) effect it is more obvious (P <
0.01)。
As shown in figure 3, model group (MC) Bacteroidetes number is obviously than normal group after mouse carries out chronic constriction depression
(NC) decline very much (P < 0.01), when use positive control drug Prozac (FH), day lily water of blooming mention (HCW) and the chrysanthemum that blooms
After dish alcohol extracting (HCE) sample, Bacteroidetes number is risen, but day lily water of blooming mention group (HCW) effect it is more obvious (P <
0.01)。
2. pair mouse intestinal microorganism belongs to horizontal influence
The relative abundance that different disposal belongs to horizontal lower mouse intestinal microbiologic population is detected, as a result as shown in the table.
Table 6 belongs to the relative abundance of mouse intestinal microbiologic population under level
Bacterium Desulfovibrio belongs to be shown with after Unidentified Lachnospiraceae category access lot of documents
For harmful Pseudomonas, quantity is lower in mouse Normal group (NC), and (MC) quantity increases considerably after depression model group,
When using positive control drug Prozac (FH), day lily water of blooming mention (HCW) and bloom day lily alcohol extracting (HCE) sample after, carefully
Bacterium Desulfovibrio belongs to and Unidentified Lachnospiraceae belongs to quantity and declined, but comprehensive analysis is opened
It is more obvious that flower day lily water proposes group (HCW) effect.
Bacterium Alloprevotella, which belongs to, to be shown to be with after Bacteroides category access lot of documents beneficial to Pseudomonas, small
Quantity is higher in mouse Normal group (NC), and (MC) quantity is but greatly reduced after depression model group, when use positive control
After medicine Prozac (FH), day lily water of blooming mention (HCW) and day lily alcohol extracting (HCE) sample of blooming, bacterium
Alloprevotella, which belongs to, and Bacteroides belongs to quantity can increased, but comprehensive analysis day lily water of blooming proposes group
(HCW) effect is more obvious.
Embodiment 5 is bloomed day lily extract capsule agent
By embodiment 1 prepare bloom day lily water extract or prepared by embodiment 2 blooms day lily alcohol extract routinely
Amount be added lactose (or starch or dextrin), be uniformly mixed be made particle or directly it is filling in capsule to obtain the final product.
Embodiment 6 is bloomed day lily extract particles agent
By embodiment 1 prepare bloom day lily water extract or prepared by embodiment 2 blooms day lily alcohol extract routinely
Dextrin is added in amount, is granulated, is sieved after dry using conventional method to obtain the final product.
Embodiment 7 is bloomed day lily extract tablet
By embodiment 1 prepare bloom day lily water extract or prepared by embodiment 2 blooms day lily alcohol extract routinely
Magnesium stearate, glycerol and modified starch is added in amount, after mixing with tabletting machine to obtain the final product.
Claims (10)
1. the preparation method for day lily extract of blooming, which is characterized in that the day lily extract of blooming is the day lily that blooms
Water extract or day lily alcohol extract of blooming；
The preparation method of the day lily water extract of blooming the following steps are included:
(1) day lily that will bloom grinds, and the normal-temperature water that 6-10 times of quality is added extracts 5-7h, and concentrated extracting solution after having extracted obtains
First medicinal extract；
(2) normal-temperature water that 6-10 times of quality is continuously added in residue extracts 2-4h, and concentrated extracting solution after having extracted obtains the second leaching
Cream；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, 24-36h will be freezed under the conditions of -80 DEG C of third medicinal extract；
(4) it after the third medicinal extract freezed being freeze-dried 72-96h, grinds and beats powder, day lily water extract of blooming is made；
The preparation method of the day lily alcohol extract of blooming the following steps are included:
(1) day lily that will bloom grinds, and the ethyl alcohol that the volumetric concentration that 6-10 times of quality is added is 80-90% extracts 5-7h, extracts
Concentrated extracting solution after complete, obtains the first medicinal extract；
(2) ethyl alcohol that the volumetric concentration that 6-10 times of quality is continuously added in residue is 80-90% extracts 2-4h, dense after having extracted
Contracting extracting solution obtains the second medicinal extract；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, 24-36h will be freezed under the conditions of -80 DEG C of third medicinal extract；
(4) it after the third medicinal extract freezed being freeze-dried 72-96h, grinds and beats powder, day lily alcohol extract of blooming is made.
2. the preparation method of day lily extract according to claim 1 of blooming, which is characterized in that the day lily that blooms
The preparation method of water extract the following steps are included:
(1) day lily that will bloom grinds, and the normal-temperature water that 8 times of quality are added extracts 6h, and concentrated extracting solution after having extracted obtains the first leaching
Cream；
(2) normal-temperature water that 8 times of quality are continuously added in residue extracts 3h, and concentrated extracting solution after having extracted obtains the second medicinal extract；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, will be freezed for 24 hours under the conditions of -80 DEG C of third medicinal extract；
(4) it is taken after the third medicinal extract freezed being freeze-dried 72h, grinds and beat powder, day lily water extract of blooming is made.
3. the preparation method of day lily extract according to claim 1 of blooming, which is characterized in that the day lily that blooms
The preparation method of alcohol extract the following steps are included:
(1) day lily that will bloom grinds, and the ethyl alcohol that the volumetric concentration that 8 times of quality are added is 85% extracts 6h, concentration after having extracted
Extracting solution obtains the first medicinal extract；
(2) ethyl alcohol that the volumetric concentration that 8 times of quality are continuously added in residue is 85% extracts 3h, concentration extraction after having extracted
Liquid obtains the second medicinal extract；
(3) the first medicinal extract and the second medicinal extract are merged into obtain third medicinal extract, will be freezed for 24 hours under the conditions of -80 DEG C of third medicinal extract；
(4) it after the third medicinal extract freezed being freeze-dried 72h, grinds and beats powder, day lily alcohol extract of blooming is made.
The day lily extract 4. one kind is bloomed, which is characterized in that use the described in any item preparation method systems of claim 1-3
.
5. the purposes of day lily extract in preparation antidepressant according to claim 4 of blooming.
6. purposes according to claim 5, which is characterized in that the antidepressant is that Firmicutes are micro- in reduction enteron aisle
The drug of biomass, improve the drug of Bacteroidetes micro organism quantity in enteron aisle, reduce Desulfovibrio in enteron aisle belong to and
The drug of Unidentified Lachnospiraceae microorganism belonging to genus quantity, improve Alloprevotella in enteron aisle belong to and
The one of which of the drug of Bacteroides microorganism belonging to genus quantity.
7. a kind of medicine composition for treating depression, which is characterized in that the medicine composition for treating depression includes as claimed in claim 4
Day lily extract of blooming as active constituent.
8. a kind of medicine composition for treating depression according to claim 7, which is characterized in that the medicine composition for treating depression
It further include pharmaceutically acceptable auxiliary material.
9. a kind of medicine composition for treating depression according to claim 8, which is characterized in that the auxiliary material is filler, collapses
Solve agent, lubricant, suspending agent, adhesive, sweetener, corrigent, preservative, matrix one or more of them.
10. a kind of medicine composition for treating depression according to claim 7, which is characterized in that the pharmaceutical dosage form is capsule
Agent, tablet, granule, powder, oral solution, pill one of which.
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