CN110522532A - A kind of utensil and its transplantation method through common oviduct transplantation embryo - Google Patents
A kind of utensil and its transplantation method through common oviduct transplantation embryo Download PDFInfo
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- CN110522532A CN110522532A CN201910851138.5A CN201910851138A CN110522532A CN 110522532 A CN110522532 A CN 110522532A CN 201910851138 A CN201910851138 A CN 201910851138A CN 110522532 A CN110522532 A CN 110522532A
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- embryo
- utensil
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- tubule
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Transplantation (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention relates to the utensil of transplanting embryo and its transplantation method technical fields; more particularly to a kind of utensil and its transplantation method of transplanting embryo; it solves compared with the mouth suction pipe that some laboratories use; it is various inconvenient and unhygienic when avoiding execute-in-place, protect the physically and mentally healthy problem of the prior art of operator.A kind of utensil through common oviduct transplantation embryo includes that sucking tip, tubule and connector are integrally formed, and sucking tip one end is connected with tubule, and tubule one end is equipped with connector.The utensil of embryo transfer of the present invention, which picks embryo from culture medium, to be come, then inside Rapid transplant to the fallopian tubal of female mammal, keep the use of operator more simple and convenient, in the method for embryo transfer provided by the invention, only 0.2ml culture medium follows embryo to be transplanted into inside the fallopian tubal of female mammal together, influences on Oviductal Fluid in fallopian tubal.
Description
Technical field
The present invention relates to the utensil of transplanting embryo and its transplantation method technical fields more particularly to a kind of through common oviduct transplantation
The utensil and its transplantation method of embryo.
Background technique
In existing embryo engineering research, method base that the embryo after the reconstructed eggs or development completed is transplanted
There was only the following two kinds in sheet: the first is that the hole left via the uterine wall after puncture is transplanted, and this method has graft
It is required that embryonic development period had in 8 cell stages~blastula stage；It is for second the cervix or fallopian tubal via uterus
The two natural holes into uterus or fallopian tubal, can be had damage embryo transfer to uterus with when first method, and big
The uterine wall of animal is thicker, is very easy in the film for injecting intrauterine wall, influences the attached plant of embryo, and by natural hole
Embryo is required with through uterine wall transplanting, but by wanting when common oviduct transplantation in natural hole to embryo when uterine neck is transplanted
It is not very high for asking, can be with to the embryo of 16 cell stages from the protokaryon phase.
In embryo transfer operation mostly in the laboratory of farm, there is contact with animal.With some laboratories
The mouth suction pipe used is compared, various inconvenient and unhygienic when avoiding execute-in-place, protects the physical and mental health of operator.
Summary of the invention
The object of the present invention is to provide a kind of utensil and its transplantation method through common oviduct transplantation embryo, solves existing skill
There is contact with animal mostly in the laboratory of farm when embryo transfer operates present in art.With some experiments
The mouth suction pipe that room uses is compared, various inconvenient and unhygienic when avoiding execute-in-place, protects the physical and mental health of operator
Problem.
To achieve the goals above, present invention employs following technical solutions:
A kind of utensil through common oviduct transplantation embryo is made of one piece by sucking tip, tubule and connector, wherein
Tubule is located between sucking tip and connector, and transition region is equipped between connector and tubule.
Preferably, the internal diameter of tubule is gradually increased from sucking tip to the direction of connector, and thin g pipe is located at sucking tip
One end internal diameter is 0.9mm, and one end internal diameter that tubule is located at connector is 4.4mm, and the pipe thickness of tubule is 0.2mm.
Preferably, the internal diameter for sucking tip is 0.9mm, and sucking tip outer diameter is 1.3mm, and the length for sucking tip can be
Any one in 5cm, 9cm, 14cm, 23cm.
Preferably, internal diameter of the connector far from tubule one end and outer diameter are respectively 4.4mm, 4.8mm.
Preferably, the internal diameter Yu outer diameter of transition region are respectively 3.9mm, 4.3mm.
A kind of transplantation method through common oviduct transplantation embryo, operating procedure are as follows；
E1, to utensil joint area inside be inserted into syringe adapter；
E2 squeezes syringe, and air is discharged, and sucking 0.2ml liquid forms fluid column c full of embryonic tube tip is moved；
E3 inhales one section of air column s1, forms air column s1, then sucks embryo and culture solution together and form fluid column b, then suck
One section of air forms air column s2, finally sucks one section of liquid and forms fluid column a, forms the layout of 3 sections of fluid columns, 2 sections of air columns in this way；
E4, embryo transfer process is is inserted into the utensil through common oviduct transplantation embryo in fimbriae tubae portion, until suitable depth,
Then syringe is pushed, is promoted in fallopian tubal together with liquid and embryo.
Preferably, utensil is in plumbness always during step e2 to step e3.
Preferably, in step e2 and e3,
The volume of fluid column c is 0.2ml,
The volume of fluid column b is 0.1ml,
The volume of fluid column a is 0.05ml.
Compared with prior art, the beneficial effects of the present invention are: the utensil of embryo transfer picks embryo from culture medium
Come, then inside the fallopian tubal of Rapid transplant to female mammal, keeps the use of operator more simple and convenient, the present invention mentions
In the method for the embryo transfer of confession, only 0.2ml culture medium follows embryo to be transplanted into the fallopian tubal of female mammal together
Face influences Oviductal Fluid in fallopian tubal；
Using transplantation method of the invention, in migration process, if air column s1 and air column s2 are not moved, operator can
To observe that embryo does not lose, on the contrary, if operator in operation, it is found that the structure of air column and fluid column is endless
Whole, then operator can consider that exception may occur in embryo, can terminate operation immediately, returns and checks whether embryo loses
It loses, or uses other embryos instead.
Detailed description of the invention
Technical solution in order to illustrate the embodiments of the present invention more clearly, below will be to needed in embodiment description
Attached drawing is briefly described, it should be apparent that, drawings in the following description are some embodiments of the invention, general for this field
For logical technical staff, without creative efforts, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is overall diagram of the present invention；
Fig. 2 is side elevational detail view of the present invention；
Fig. 3 is main view detail view of the present invention；
Fig. 4 is operation of the present invention schematic diagram.
In figure: 1, sucking tip；2 tubules,；3, connector.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Referring to Fig.1-4, a kind of utensil through common oviduct transplantation embryo is integrally formed by sucking tip 1, tubule 2 and connector 3
It forms, wherein
Tubule 2 is located between sucking tip 1 and connector 3, and transition region G1 is equipped between connector 3 and tubule 2.
Further, the internal diameter of tubule 2 is gradually increased from sucking tip 1 to the direction of connector 3, and tubule 2 is located at sucking point
One end internal diameter in portion 1 is 0.9mm, and one end internal diameter that tubule 2 is located at connector 3 is 4.4mm, and the pipe thickness of tubule 2 is 0.2mm,
The position that bubble and the locking fluid column of section containing embryo 2 are formed in this way in tubule 2, is allowed to arbitrarily to slide, convenient for subsequent
The embryo for carrying out culture solution transplants.
Further, the internal diameter for sucking tip 1 is 0.9mm, and sucking 1 outer diameter of tip is 1.3mm, and the length of sucking tip 1 can
Think any one in 5cm, 9cm, 14cm, 23cm.
Further, internal diameter of the connector 3 far from 2 one end of tubule and outer diameter are respectively 4.4mm, 4.8mm.
Further, the internal diameter of transition region G1 and outer diameter are respectively 3.9mm, 4.3mm.
A kind of transplantation method through common oviduct transplantation embryo, operating procedure are as follows；
E1 is inserted into syringe adapter to 3 part interior of utensil connector；
E2 squeezes syringe, and air is discharged, and sucking 0.2ml liquid forms fluid column c full of embryonic tube tip is moved；
E3 inhales one section of air column s1, forms air column s1, then sucks embryo and culture solution together and form fluid column b, then suck
One section of air forms air column s2, finally sucks one section of liquid and forms fluid column a, forms the layout of 3 sections of fluid columns, 2 sections of air columns in this way；
E4, embryo transfer process is is inserted into the utensil through common oviduct transplantation embryo in fimbriae tubae portion, until suitable depth,
Then syringe is pushed, is promoted in fallopian tubal together with liquid and embryo.
Further, utensil is in plumbness always during step e2 to step e3.
In further step e2 and e3,
The volume of fluid column c is 0.2ml,
The volume of fluid column b is 0.1ml,
The volume of fluid column a is 0.05ml.
Embodiment one: (by taking Pig embryos are transplanted as an example),
The liquid used is holding liquid 1, moves length of tube using embryo and is 23cm and connects 1m l syringe, successively sucks
0.2ml holding liquid 1 forms fluid column c, then sucks one section of gas formation air column s2, again together with holding liquid 1 and embryo one
It plays sucking to form fluid column b, form air column s1 followed by one section of gas of sucking, finally sucks holding liquid 1 and form fluid column a.Transplanting
When, clutch fallopian tubal and embryo transplantation tube, make to be completely embedded between it, and push syringe, make holding liquid 1 and embryo from
Fallopian tubal is flowed into embryo transplantation tube, and embryo transplantation tube is finally extracted from fallopian tubal.
Embodiment two: (by taking sheep embryo transfer as an example),
The liquid used is holding liquid 2, moves length of tube using embryo and is 9cm and connects 1ml syringe, successively sucks
0.2ml holding liquid 2 forms fluid column c, then sucks one section of gas formation air column s2, again together with holding liquid 2 and embryo one
It plays sucking to form fluid column b, form air column s1 followed by one section of gas of sucking, finally sucks holding liquid 2 and form fluid column a.Transplanting
When, clutch fallopian tubal and embryo transplantation tube, make to be completely embedded between it, and push syringe, make holding liquid 2 and embryo from
Fallopian tubal is flowed into embryo transplantation tube, and embryo transplantation tube is finally extracted from fallopian tubal.
Optimum implementation three: (by taking monkey fallopian tubal embryo transfer as an example),
The liquid used is holding liquid 3, moves length of tube using embryo and is 5cm and connects 1m l syringe, successively sucks
0.2ml holding liquid 3 forms fluid column c, then sucks one section of gas formation air column s2, again together with holding liquid 3 and embryo one
It plays sucking to form fluid column b, form air column s1 followed by one section of gas of sucking, finally sucks holding liquid 3 and form fluid column a, transplanting
When, clutch fallopian tubal and embryo transplantation tube, make to be completely embedded between it, and push syringe, make holding liquid 3 and embryo from
Fallopian tubal is flowed into embryo transplantation tube, and embryo transplantation tube is finally extracted from fallopian tubal.
Optimum implementation four: (by taking monkey Nonoperative method transcervical embryo transfer as an example),
The liquid used is holding liquid 3, moves length of tube using embryo and is 14cm and connects 1ml syringe, successively sucks
0.2ml holding liquid 3 forms fluid column c, then sucks one section of gas formation air column s2, again together with holding liquid 3 and embryo one
It plays sucking to form fluid column b, form air column s1 followed by one section of gas of sucking, finally sucks holding liquid 3 and form fluid column a.Transplanting
When, clutch fallopian tubal and embryo transplantation tube, make to be completely embedded between it, and push syringe, make holding liquid 3 and embryo from
Fallopian tubal is flowed into embryo transplantation tube, and embryo transplantation tube is finally extracted from fallopian tubal.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and what is described in the above embodiment and the description is only the present invention
Principle, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these variation and
Improvement is both fallen in the range of claimed invention.The present invention claims protection scope by appended claims and its
Equivalent defines.
Claims (8)
1. a kind of utensil through common oviduct transplantation embryo, which is characterized in that be integrally formed by sucking tip 1, tubule 2 and connector 3
It forms, wherein
The tubule 2 is equipped between the connector 3 and the tubule 2 between the sucking tip 1 and the connector 3
Transition region G1.
2. a kind of utensil through common oviduct transplantation embryo according to claim 1, which is characterized in that the tubule 2 it is interior
Diameter is gradually increased from sucking tip 1 to the direction of connector 3, and it is 0.9mm, tubule that tubule 2, which is located at one end internal diameter of sucking tip 1,
The 2 one end internal diameters for being located at connector 3 are 4.4mm, and the pipe thickness of the tubule 2 is 0.2mm.
3. a kind of utensil through common oviduct transplantation embryo according to claim 1, which is characterized in that the sucking tip 1
Internal diameter be 0.9mm, sucking 1 outer diameter of tip be 1.3mm, suck tip 1 length can be 5cm, 9cm, 14cm, 23cm in
Any one.
4. a kind of utensil through common oviduct transplantation embryo according to claim 1, which is characterized in that the connector 3 is separate
The internal diameter and outer diameter of 2 one end of tubule are respectively 4.4mm, 4.8mm.
5. a kind of utensil through common oviduct transplantation embryo according to claim 1, which is characterized in that the transition region G1's
Internal diameter and outer diameter are respectively 3.9mm, 4.3mm.
6. a kind of transplantation method through common oviduct transplantation embryo, which is characterized in that including a kind of through fallopian tubal of claim 1-5
The utensil of transplanting embryo, operating procedure are as follows；
E1 is inserted into syringe adapter to 3 part interior of utensil connector；
E2 squeezes syringe, and air is discharged, and sucking 0.2ml liquid forms fluid column c full of embryonic tube tip is moved；
E3 inhales one section of air column s1, forms air column s1, then sucks embryo and culture solution together and form fluid column b, then suck one section
Air forms air column s2, finally sucks one section of liquid and forms fluid column a, forms the layout of 3 sections of fluid columns, 2 sections of air columns in this way；
E4, embryo transfer process is is inserted into the utensil through common oviduct transplantation embryo in fimbriae tubae portion, until suitable depth, then
Syringe is pushed, is promoted in fallopian tubal together with liquid and embryo.
7. a kind of transplantation method through common oviduct transplantation embryo according to claim 4, which is characterized in that the step e2
It is in plumbness always to utensil during step e3.
8. a kind of transplantation method through common oviduct transplantation embryo according to claim 4, which is characterized in that the step e2
In e3,
The volume of the fluid column c is 0.2ml,
The volume of fluid column b is 0.1ml,
The volume of fluid column a is 0.05ml.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910851138.5A CN110522532A (en) | 2019-09-10 | 2019-09-10 | A kind of utensil and its transplantation method through common oviduct transplantation embryo |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910851138.5A CN110522532A (en) | 2019-09-10 | 2019-09-10 | A kind of utensil and its transplantation method through common oviduct transplantation embryo |
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| CN2360049Y (en) * | 1998-05-08 | 2000-01-26 | 上海市儿童医院上海医学遗传研究所 | Sheep multicelluter embryo tramsplanter |
| JP2005261413A (en) * | 2004-03-19 | 2005-09-29 | Yamaguchi Technology Licensing Organization Ltd | Animal embryo freezing and storing liquid and animal embryo freezing and storing method using the same |
| CN2824871Y (en) * | 2005-01-18 | 2006-10-11 | 华中农业大学 | An embryo manipulation device |
| CN1654635A (en) * | 2005-01-28 | 2005-08-17 | 中国农业大学 | Vitrification freezing, simple defreezing and direct implanting method for embryo |
| US20080200752A1 (en) * | 2007-02-15 | 2008-08-21 | Pieraldo Inaudi | Method for intrauterine embryo transfer and relevant device |
| CN101785712A (en) * | 2010-02-03 | 2010-07-28 | 余文莉 | Method for enhancing cow transplanting pregnancy rate by double-half embryo transplant |
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Application publication date: 20191203 |
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