CN105105866A - Embryo transplantation tool and application - Google Patents
Embryo transplantation tool and application Download PDFInfo
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- CN105105866A CN105105866A CN201510564256.XA CN201510564256A CN105105866A CN 105105866 A CN105105866 A CN 105105866A CN 201510564256 A CN201510564256 A CN 201510564256A CN 105105866 A CN105105866 A CN 105105866A
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Abstract
The invention relates to an embryo transplantation tool and an application, wherein the embryo transplantation tool is constituted of a transplantation outer tube, an outer tube steel wire, a transplantation inner tube and a stainless steel guide wire with a bent front end. The transplantation outer tube has plasticity, wherein the front end is equipped with an accurate scale, so the embryo transplantation tool is suitable for a bent cervix and can measure an insertion depth into an uterus; and the transplantation inner tube is soft, brings small damages to an endometrium and has no embryo toxicity, and the rear end is equipped with the scale which can objectively reflect the length of the transplantation inner tube outside the outer tube. The tool can be used to transplant a goat embryo, reduce wounds caused by a goat embryo transplantation operation, reduce tissue adhesion and increase use efficiency of a receptor.
Description
Technical field
The invention belongs to field of mechanical technique, be specifically related to a kind of embryo transfer instrument and application thereof.
Background technology
China is a large agricultural country, but the production efficiency of animal husbandry is for a long time low.This situation major reason is caused to be shortage due to excellent breeding stock.How to realize the universal of improved seeds, one of them approach adopts superovulation and embryo transfer technology exactly.But the embryo transfer now for excellent breeding stock mainly adopts modus operandi (laparoscopic surgery or directly abdominal) to carry out embryo transfer, the consequence caused like this be receptor sheep due to operation reason cause that pelvic cavity is hemorrhage, adhesion, infection risk be large, because pelvic adhesion or infection just must be eliminated after utilizing 1-2 time, reduce the utilization ratio of receptor sheep, also improve production cost simultaneously.Utilize embryo transplantation tube to have non-invasive by cervix uteri temporary dwelling palace intracavity embryo transfer, sheep recovers fast, can repeatedly reuse receptor sheep.
Application number be 201420374619.4 patent of invention also disclose one " a kind of instrument of auxiliary sheep embryo transfer ", this embryo transfer instrument, when specifically using, easily bends, cause insert in pipe time be obstructed.
Summary of the invention
The object of the present invention is to provide a kind of embryo transfer instrument and application thereof, Rapid transplant can be carried out to donor embryo, thus reach the excellent poultry of fast-propagation kind of object.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of embryo transfer instrument, comprise and transplant the rustless steel seal wire that in outer tube, outer tube steel wire, transplanting, pipe and front end bend, in described transplanting, the inside of outer tube transplanted by pipe, and described outer tube steel wire is located at transplanting outer tube, the inside of pipe in the rustless steel seal wire that front end bends is located at and transplants.
In aforementioned embryo transfer instrument, described transplanting outer tube comprises conduit and joint, described transplanting outer pipe range 277.00mm, the long 250.00mm of described conduit, the external diameter of conduit is 2.00mm, internal diameter is 1.65mm, the long 35.00mm of described joint, its internal diameter diminishes to nearly conduit place gradually by away from conduit, and being specially away from conduit place internal diameter is 4.10mm, close conduit place internal diameter is 1.65mm, and described joint and conduit form non-disconnectable combination.
In aforementioned embryo transfer instrument, described joint and conduit are made up of polyethylene.
In aforementioned embryo transfer instrument, described outer tube steel wire is made up of steel wire and handle, the long 201.00mm of described steel wire, the long 1.50mm of diameter; The long 24.00mm of described handle, the long 6.40mm of diameter; Described steel wire and handle form non-disconnectable combination; Described handle is made up of polyethylene, and described steel wire is stainless steel silk.
In aforementioned embryo transfer instrument, covering without fetal toxicity material outside described steel wire is Low Density Polyethylene, silica gel.
Preferably, in aforementioned embryo transfer instrument, described is silica gel without fetal toxicity material.
In aforementioned embryo transfer instrument, in described transplanting, pipe comprises conduit and joint; The long 300.00mm of described conduit, external diameter 1.50mm, internal diameter 0.50mm; The long 35.00mm of described joint, its internal diameter diminishes to nearly conduit place gradually by away from conduit, and being specially away from conduit place internal diameter is 38.00mm, is 0.50mm near conduit place internal diameter; Described transplanting inner tube material is made by without fetal toxicity silica gel tube, and described joint is made up of polyethylene.
The invention also discloses a kind of application of aforementioned embryo transfer instrument, the embryo transfer instrument described in utilization is transplanted Goat Embryos, specific as follows:
(1) according to the superovulation time of meat sheep used as donor, estrus synchronization is carried out to acceptor ewe;
(2) to acceptor ewe sterilization before transplanting, expose the cervix uteri of sheep with vaginal dilator, wipe cervical mucus;
(3) cervix uteri is cleaned with culture fluid;
(4) outer tube steel wire is inserted in transplanting outer tube, and transplanting outer tube is inserted into intrauterine by goat cervix uteri; Transplanting outer tube enters intrauterine to be stopped immediately, ensures that endometrium sustains damage minimum; If ewe cervix uteri is relatively tighter, or bend ratio is comparatively serious, then first with the logical cervix uteri quickly of outer tube steel wire, extracted immediately by cervix uteri, and then with transplanting outer tube intron in utero.
(5) staff connects transplanting inner pipe joint with 1ml syringe, guarantees to connect closely, air tight;
(6) first inhale 1cm and transplant liquid, every 0.5cm air, then start to inhale embryo, in embryo enters and transplants, Guan Zhonghou inhales 0.5cm air again, finally inhales 1cm and transplants liquid;
(7) take out the outer tube steel wire transplanted in outer tube, pipe in transplanting is inserted into and transplants in outer tube, add the degree of depth into uterus according to the dial gauge transplanted on interior pipe; When arriving correct position, the embryo transplanted in interior pipe is driven into intrauterine, and keeps 30s;
(8) pipe in transplanting and transplanting outer tube are extracted from the uterus of goat simultaneously, whether test under microscope has embryo to be bonded on tube wall, determines whether again to transplant;
(9) after having transplanted, by the isolated rearing of receptor sheep;
Whether the monitoring of (10) 30 days B ultrasonic is conceived, and unpregnancy ewe enters and nextly utilizes the cycle.
Embryo medium of the present invention and transplanting liquid are all bought from market.
The invention has the beneficial effects as follows:
Realize the non-invasive of Embryo Transfer in Goat, improve the interest rate of donor.Improve economic benefit.
The capable transplantation of embryo of transcervical has incomparable advantage compared with traditional transplantation of embryo: one is non-invasive, and speed of transplanting is fast; Two is to cause hemorrhage, reduces the risk of pelvic adhesion and infection, and later stage medicine cost drops into low; Three is that receptor sheep can be recycled, and reduces production cost.Therefore, adopt this embryo transfer instrument to carry out embryo transfer and can reach the excellent poultry of fast-propagation kind of object, meet the direction of animal husbandry development, the input of cost in reproductive process can be reduced, improve productivity effect and economic benefit; The basic tool of this instrument or animal cloning and transgenic animal in addition.
Compared with 201420374619.4, increase a trocar sheath steel wire (surface is used without the covering of fetal toxicity material), be obstructed when bending phenomenon occurring thus occurs slotting interior pipe when can effectively prevent outer tube from entering cervix uteri, cause the situation of graft failure; The present invention adopts rustless steel seal wire to be mainly used in the tighter sheep of cervix uteri, first logical once with rustless steel seal wire, then can be easier during plug in outer tube, and bending phenomenon not easily occurs.
The present invention transplants outer tube and has plasticity, and front end has exact scale, is convenient to the cervix uteri that earthing bends and measures the degree of depth entering uterus simultaneously; In transplanting, pipe is soft, and little to endometrial impairment, without fetal toxicity, rear end has scale, can objectively respond pipe in transplanting and go out the length of outer tube.The wound that during minimizing Embryo Transfer in Goat, operation causes, reduces tissue adhesion, improves receptor service efficiency.
Accompanying drawing explanation
Fig. 1 is embryo transfer tool construction schematic diagram of the present invention.
Fig. 2 is that the present invention transplants outer tube structure schematic diagram.
Fig. 3 is that the present invention transplants outer tube structure of steel wire schematic diagram.
Fig. 4 is that the present invention transplants inner tube structure schematic diagram.
Fig. 5 is the rustless steel seal wire schematic diagram that front end of the present invention bends.
In figure, 1-transplants outer tube, 2-outer tube steel wire, pipe in 3-transplants, the rustless steel seal wire that 4-front end is bending, 5-conduit, 6-joint, 7-steel wire, 8-handle.
Detailed description of the invention
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
Embodiment 1
A kind of embryo transfer instrument, as shown in Figure 1, comprise and transplant the rustless steel seal wire 4 (concrete shape and structure are as shown in Figure 5) that in outer tube 1, outer tube steel wire 2, transplanting, pipe 3 and front end bend, in described transplanting, pipe 1 is located at the inside of transplanting outer tube 3, described outer tube steel wire 2 is located at transplants outer tube 1 inside, the inside of pipe 3 in the rustless steel seal wire 4 that front end bends is located at and transplants.
As shown in Figure 2, described transplanting outer tube 1 comprises conduit 5 and joint 6, the long 277.00mm of described transplanting outer tube 1, the long 250.00mm of described conduit 5, the external diameter of conduit 5 is 2.00mm, internal diameter is 1.65mm, the long 35.00mm of described joint 6, its internal diameter diminishes to nearly conduit place gradually by away from conduit, and being specially away from conduit place internal diameter is 4.10mm, close conduit place internal diameter is 1.65mm, and described joint 5 forms non-disconnectable combination with conduit 6.Described joint 5 and conduit 6 are made up of polyethylene.
As shown in Figure 3, described outer tube steel wire 2 is made up of steel wire 7 and handle 8, the long 201.00mm of described steel wire 7, the long 1.50mm of diameter; The long 24.00mm of described handle 8, the long 6.40mm of diameter; Described steel wire 7 forms non-disconnectable combination with handle 8; Described handle 8 is made up of polyethylene, and described steel wire 7 is stainless steel silk.Covering without fetal toxicity material outside described steel wire 7 is Low Density Polyethylene or silica gel.
As shown in Figure 4, in described transplanting, pipe 3 comprises conduit 5 and joint 6; The long 300.00mm of described conduit 5, external diameter 1.50mm, internal diameter 0.50mm; The long 35.00mm of described joint 6, its internal diameter diminishes to nearly conduit place gradually by away from conduit, and being specially away from conduit place internal diameter is 38.00mm, is 0.50mm near conduit place internal diameter; In described transplanting, pipe 3 material is made by without fetal toxicity silica gel tube, and described joint 6 is made up of polyethylene.
Embodiment 2
Embryo transfer instrument is utilized to transplant Goat Embryos, specific as follows:
(1) according to the superovulation time of meat sheep used as donor, estrus synchronization is carried out to acceptor ewe;
(2) to acceptor ewe sterilization before transplanting, expose the cervix uteri of sheep with vaginal dilator, wipe cervical mucus;
(3) cervix uteri is cleaned with culture fluid;
(4) outer tube steel wire 2 is inserted in transplanting outer tube 1, and transplanting outer tube 1 is inserted into intrauterine by goat cervix uteri; Transplanting outer tube 1 enters intrauterine to be stopped immediately, ensures that endometrium sustains damage minimum; If ewe cervix uteri is relatively tighter, or bend ratio is comparatively serious, then first with the logical cervix uteri quickly of outer tube steel wire 2, extracted immediately by cervix uteri, and then with transplanting outer tube 1 intron in utero.
(5) staff connects pipe 3 joint in transplanting with 1ml syringe, guarantees to connect closely, air tight;
(6) first inhale 1cm and transplant liquid, every 0.5cm air, then start to inhale embryo, embryo enters to transplant after in interior pipe 3 and inhales 0.5cm air again, finally inhales 1cm and transplants liquid;
(7) take out the outer tube steel wire 2 transplanted in outer tube, pipe 3 in transplanting is inserted into and transplants in outer tube 2, add the degree of depth into uterus according to the dial gauge transplanted on interior pipe 3; When arriving correct position, the embryo transplanted in interior pipe 3 is driven into intrauterine, and keeps 30s;
(8) pipe 3 in transplanting and transplanting outer tube 1 are extracted from the uterus of goat simultaneously, whether test under microscope has embryo to be bonded on tube wall, determines whether again to transplant;
(9) after having transplanted, by the isolated rearing of receptor sheep;
Whether the monitoring of (10) 30 days B ultrasonic is conceived, and unpregnancy ewe enters and nextly utilizes the cycle.
Claims (8)
1. an embryo transfer instrument, it is characterized in that, comprise and transplant the rustless steel seal wire (4) that in outer tube (1), outer tube steel wire (2), transplanting, pipe (3) and front end bend, in described transplanting, pipe (1) is located at the inside of transplanting outer tube (3), described outer tube steel wire (2) is located at transplants outer tube (1) inside, the inside of pipe (3) in the rustless steel seal wire (4) that front end bends is located at and transplants.
2. a kind of embryo transfer instrument according to claim 1, it is characterized in that, described transplanting outer tube (1) comprises conduit (5) and joint (6), the long 277.00mm of described transplanting outer tube (1), described conduit (5) long 250.00mm, the external diameter of conduit (5) is 2.00mm, internal diameter is 1.65mm, described joint (6) long 35.00mm, its internal diameter diminishes to nearly conduit place gradually by away from conduit, being specially away from conduit place internal diameter is 4.10mm, close conduit place internal diameter is 1.65mm, described joint (5) and conduit (6) form non-disconnectable combination.
3. a kind of embryo transfer instrument according to claim 2, is characterized in that, described joint (5) and conduit (6) are made up of polyethylene.
4. a kind of embryo transfer instrument according to claim 1, it is characterized in that, described outer tube steel wire (2) is made up of steel wire (7) and handle (8), described steel wire (7) long 201.00mm, the long 1.50mm of diameter; Described handle (8) long 24.00mm, the long 6.40mm of diameter; Described steel wire (7) and handle (8) form non-disconnectable combination; Described handle (8) is made up of polyethylene, and described steel wire (7) is stainless steel silk.
5. a kind of embryo transfer instrument according to claim 4, is characterized in that, covering without fetal toxicity material outside described steel wire (7) is Low Density Polyethylene, silica gel.
6. a kind of embryo transfer instrument according to claim 5, is characterized in that, described is silica gel without fetal toxicity material.
7. a kind of embryo transfer instrument according to claim 1, is characterized in that, in described transplanting, pipe (3) comprises conduit (5) and joint (6); Described conduit (5) long 300.00mm, external diameter 1.50mm, internal diameter 0.50mm; Described joint (6) long 35.00mm, its internal diameter diminishes to nearly conduit place gradually by away from conduit, and being specially away from conduit place internal diameter is 38.00mm, is 0.50mm near conduit place internal diameter; In described transplanting, pipe (3) material is made by without fetal toxicity silica gel tube, and described joint (6) is made up of polyethylene.
8. the application of the embryo transfer instrument as described in any one of claim 1 ~ 7, is characterized in that, the embryo transfer instrument described in utilization is transplanted Goat Embryos, specific as follows:
(1) according to the superovulation time of meat sheep used as donor, estrus synchronization is carried out to acceptor ewe;
(2) to acceptor ewe sterilization before transplanting, expose the cervix uteri of sheep with vaginal dilator, wipe cervical mucus;
(3) cervix uteri is cleaned with culture fluid;
(4) outer tube steel wire (2) is inserted into transplants in outer tube (1), and outer tube (1) will be transplanted be inserted into intrauterine by goat cervix uteri; Transplanting outer tube (1) enters intrauterine and stops immediately, ensures that endometrium sustains damage minimum; If ewe cervix uteri is relatively tighter, or bend ratio is comparatively serious, then first use the logical cervix uteri quickly of outer tube steel wire (2), extracted immediately by cervix uteri, and then with transplanting outer tube (1) intron in utero;
(5) staff connects pipe (3) joint in transplanting with 1ml syringe, guarantees to connect closely, air tight;
(6) first inhale 1cm and transplant liquid, every 0.5cm air, then start to inhale embryo, embryo manages after in (3) in entering and transplanting and inhales 0.5cm air again, finally inhales 1cm and transplants liquid;
(7) take out the outer tube steel wire (2) transplanted in outer tube, in transplanting, pipe (3) is inserted into and transplants in outer tube (2), adds the degree of depth into uterus according to the dial gauge on pipe (3) in transplanting; When arriving correct position, the embryo transplanted in interior pipe (3) is driven into intrauterine, and keeps 30s;
(8) extract transplanting interior pipe (3) and transplanting outer tube (1) from the uterus of goat simultaneously, whether test under microscope has embryo to be bonded on tube wall, determines whether again to transplant;
(9) after having transplanted, by the isolated rearing of receptor sheep;
Whether the monitoring of (10) 30 days B ultrasonic is conceived, and unpregnancy ewe enters and nextly utilizes the cycle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510564256.XA CN105105866A (en) | 2015-09-07 | 2015-09-07 | Embryo transplantation tool and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510564256.XA CN105105866A (en) | 2015-09-07 | 2015-09-07 | Embryo transplantation tool and application |
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| CN105105866A true CN105105866A (en) | 2015-12-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510564256.XA Pending CN105105866A (en) | 2015-09-07 | 2015-09-07 | Embryo transplantation tool and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107485464A (en) * | 2017-09-13 | 2017-12-19 | 马博 | A kind of embryo transplanter |
| CN110063776A (en) * | 2018-01-23 | 2019-07-30 | 河北农业大学 | Human embryo transplanting device |
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| US4178936A (en) * | 1976-08-02 | 1979-12-18 | Milk Marketing Board | Apparatus for use in collecting eggs from animals |
| CN1759815A (en) * | 2005-11-21 | 2006-04-19 | 北京锦绣大地农业股份有限公司 | Method for collecting ovum of living body of cow through endoscope |
| CN1883419A (en) * | 2006-06-29 | 2006-12-27 | 蒋和生 | Animal embryo collection apparatus for buffalo |
| CN1939238A (en) * | 2005-09-28 | 2007-04-04 | 周强 | Non-operation embryo transplanter for ox |
| US20080200752A1 (en) * | 2007-02-15 | 2008-08-21 | Pieraldo Inaudi | Method for intrauterine embryo transfer and relevant device |
| CN201213834Y (en) * | 2008-07-07 | 2009-04-01 | 毕宜法 | Embryo transplantation tube |
| CN203220461U (en) * | 2013-04-25 | 2013-10-02 | 青海省畜牧兽医科学院 | Cannula assembly assisting bovine embryo transfer |
| CN203970613U (en) * | 2014-07-08 | 2014-12-03 | 河南省农业科学院畜牧兽医研究所 | A kind of instrument of auxiliary sheep embryo transfer |
-
2015
- 2015-09-07 CN CN201510564256.XA patent/CN105105866A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4178936A (en) * | 1976-08-02 | 1979-12-18 | Milk Marketing Board | Apparatus for use in collecting eggs from animals |
| CN1939238A (en) * | 2005-09-28 | 2007-04-04 | 周强 | Non-operation embryo transplanter for ox |
| CN1759815A (en) * | 2005-11-21 | 2006-04-19 | 北京锦绣大地农业股份有限公司 | Method for collecting ovum of living body of cow through endoscope |
| CN1883419A (en) * | 2006-06-29 | 2006-12-27 | 蒋和生 | Animal embryo collection apparatus for buffalo |
| US20080200752A1 (en) * | 2007-02-15 | 2008-08-21 | Pieraldo Inaudi | Method for intrauterine embryo transfer and relevant device |
| CN201213834Y (en) * | 2008-07-07 | 2009-04-01 | 毕宜法 | Embryo transplantation tube |
| CN203220461U (en) * | 2013-04-25 | 2013-10-02 | 青海省畜牧兽医科学院 | Cannula assembly assisting bovine embryo transfer |
| CN203970613U (en) * | 2014-07-08 | 2014-12-03 | 河南省农业科学院畜牧兽医研究所 | A kind of instrument of auxiliary sheep embryo transfer |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107485464A (en) * | 2017-09-13 | 2017-12-19 | 马博 | A kind of embryo transplanter |
| CN107485464B (en) * | 2017-09-13 | 2019-06-07 | 马博 | A kind of embryo transplanter |
| CN110063776A (en) * | 2018-01-23 | 2019-07-30 | 河北农业大学 | Human embryo transplanting device |
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