CN104720928A - Artificial insemination method for cow sex control frozen semens - Google Patents
Artificial insemination method for cow sex control frozen semens Download PDFInfo
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- CN104720928A CN104720928A CN201510164831.7A CN201510164831A CN104720928A CN 104720928 A CN104720928 A CN 104720928A CN 201510164831 A CN201510164831 A CN 201510164831A CN 104720928 A CN104720928 A CN 104720928A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
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Abstract
The invention discloses an artificial insemination method for cow sex control frozen semens. The artificial insemination method includes the following steps that semens, containing X chromosomes, of high-quality male cows are selected and stored inside a high-vacuum-insulation liquid nitrogen tank which is arranged on a wood backing plate which is clean, dry and ventilative; healthy bred cows and high-productivity multiparous female cows are selected, and mating semen deposition is performed on the female cows in the oestrum through the combination between exterior observation and a rectal palpation method. The method is simple and easy to implement, the sex control frozen semens are used for matching, in this way, the birth rate of cow calves is greatly increased to about 90%, the cow generation interval time is greatly shortened, the expanding propagation speed of the cows is increased, the overall production performance of the cows is improved, the cost is lowered, economic benefits are increased, and good application prospects are achieved.
Description
Technical field
The present invention relates to one and test-tube technology is carried out to captive animal, particularly relate to the control of milk obstinacy and freeze smart test-tube method.
Background technology
Property control freeze essence refer to by spermatozoa isolation instrument by bull semen containing after X chromosome is effectively separated with Y chromosome sperm, after freezing containing the sperm subpackage of X chromosome, for the artificial insemination of cattle, thus make cow pregnancy produce the technology of cow calf; This difference according to sperm X, Y chromosome and subpackage freezing freeze essence just the control of crying property freeze essence.This technology is by intervening artificially and making jenny breed the one breeding new technique of required sex offspring by the expectation of people.
Sex controll raising technology is after regular insemination and embryo transfer, the third time revolution of animal breeding improving technology.At present, it is that current raising cow reproduction is the most advanced, the most real that smart technology of artificial insemination is frozen in the control of milk obstinacy, and is a new and high technology that can play cow reproduction potentiality to greatest extent.Property control freeze smart technology of artificial insemination and promote the use of and add female-calving rate, shorten time milch cow generation inteval, milch cow reproductive speed accelerated, positive effect will be played to the hereditary character of improvement milch cow.
Summary of the invention
The object of the present invention is to provide and can improve female calf natality, smart test-tube method is frozen in the milk obstinacy control increasing economic benefit.
For achieving the above object, the present invention is by the following technical solutions:
Smart test-tube method is frozen in the control of milk obstinacy, comprises the following steps:
(1) sperm of high-quality bull containing X chromosome is chosen
Choose bull and reach more than 0.4 containing the motility of sperm of X chromosome, every agent is frozen smart rectilinear motion sperm quantity and is reached more than 2,200,000, and avirulent bacteria number is lower than 800/ml;
(2) seminal fluid is stored
To the Sperm storage of high-quality bull X chromosome be chosen in the liquid nitrogen container that fine vacuum is incubated, and liquid nitrogen container is placed on wooden backing plate that is clean, dry, that ventilate;
(3) cow is joined in strict screening
Primary election growing cattle;
Primary election growing cattle is at 16 ~ 18 monthly ages, ginseng is joined cow and is selected excellent high yield cow and offspring thereof as use object, body weight reaches more than 350kg, nutrition and being in a good state of health, the growth of livestock is moderate, physically fit, without reproductive capability disease, for cervix uteri, the comparatively thin or hard person of cervix uteri does not select, and conception rate can reach more than 70%, can increase substantially Cow ratio and economic benefit within a short period of time;
Select multiparous cow;
Select healthy, without reproductive disease, without hard to settle history, without difficult labour history, without placenta retention history and other relevant diseases, more than 50 days puerperal, normal high yield cow of oestrusing, multiparity cattle conception rate is only about 30%-50%, and economic benefit is relatively low;
(4) breeding semen deposition
Outside observation finds that cow accepted mounting or other cattle of mounting after 12 ~ 16 hours, when per rectum checks that all are normal in uterus, follicular development is ripe or cow quietly to stand, accept mounting and vaginal orifice and flow out transparent input seminal fluid when there is strong stringiness mucus;
Described step (2) stores seminal fluid as follows: the liquid nitrogen horizontal face in liquid nitrogen container should remain on more than 20cm, when being low to moderate 18cm, timely interpolation liquid nitrogen, differentiate liquid nitrogen horizontal with platform scale, that takes out except these needs freezes except essence, and other freeze essence can not mention on below tank mouth 3.5cm line, when searching froze essence more than 10 seconds, barrelling will be divided to put back in liquid nitrogen, and then mention searching, to keep the cold degree freezing essence; Take out rearmounted 38 ~ 40 DEG C of water-baths, 5 seconds are instantaneous thaws; Each bull semen should be placed in same point of barrelling, and has subpackage inventory, inventory comprises bull number, quantity and takes record; When cleaning liquid nitrogen container, will freeze essence and be quickly transferred to the clean liquid nitrogen container of ready placement arranged side by side, freezing the essence exposed time outside tank can not more than 5 second.
The strong stringiness mucus of described step (4), haircuts lift reach 6 ~ 8 times, draw 3 ~ 5 centimeter length to be semen deposition window of opportunitys.
The method of described step (4) breeding semen deposition comprises the steps:
The sterilization of cow pudendum is carried out before A, semen deposition, drying and the health (thin film protective jacket should be used outside spermatic gun) of the clean and semen deposition utensil around cattle vaginal orifice is kept in semen deposition, spermatic gun uses thin film protective jacket outward, avoid wound as far as possible, use harmless to sperm, non-stimulated to reproductive tract lubricant;
B, outside observational method carry out detection of oestrus in conjunction with examination per rectum method, outside observational method, find that cow accepted mounting or other cattle of mounting after 12 ~ 16 hours, carry out examination per rectum, during examination per rectum, maneuver wants light, ovary is not easily affected to cause follicle to damage, touching follicle maturity state. follicular development is ripe, namely follicle is given prominence to, round and smooth, fluctuation obviously, have one touch namely broken sense after 1 ~ 2 hour or cow quietly to stand, accept mounting and vaginal orifice outflow is transparent when having strong stringiness mucus, for the estrus of cow phase, Inseminated time;
C, to estrus cow input seminal fluid, after during input seminal fluid, seme-deposition device upwards enters vaginal orifice vestibule section separately with 35 ~ miter angle degree, slightly forward downward enters vagina cervix uteri section; The whole gesture holding cervix uteri is soft, before seme-deposition device enters cervix uteri, cervix uteri can be leaned against on pelvis limit, and the vaginal wall extruded gently around cervix uteri, seme-deposition device is made to enter cervical orifice, the injection position of seminal fluid is the joint portion of body of uterus and cornua uteri, is determining to inject the errorless rear injection seminal fluid in position, seminal fluid can be injected the large turn of bilge of cornua uteri of ovulation side.
D, completing first service, when touching the ovulation of follicle Delay in Diagnosis, needing according to the method described above through the semen deposition of row second time.
The cow of being joined that the row breeding that progresses greatly is frozen in the control of employing property selects growing cattle, and adopts the semen deposition mode of, is most preferred embodiment.
The invention has the beneficial effects as follows: the method is simple to operation, usability control frozen semen substantially increases the natality of female calf, the natality of female calf brings up to nearly 90%, greatly shorten time milch cow generation inteval, milch cow reproductive speed is accelerated, the integral production performance of milch cow is improved, reduces cost, add economic benefit, have a good application prospect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further elaborated.
Smart test-tube method is frozen in the control of milk obstinacy, comprises the following steps:
(1) sperm of high-quality bull containing X chromosome is chosen
Choose bull and reach more than 0.4 containing the motility of sperm of X chromosome, every agent is frozen smart rectilinear motion sperm quantity and is reached more than 2,200,000, and avirulent bacteria number is lower than 800/ml;
(2) seminal fluid is stored
To the Sperm storage of high-quality bull X chromosome be chosen in the liquid nitrogen container that fine vacuum is incubated, and liquid nitrogen container is placed on wooden backing plate that is clean, dry, that ventilate; Store seminal fluid as follows: the liquid nitrogen horizontal face in liquid nitrogen container should remain on more than 20cm, when being low to moderate 18cm, timely interpolation liquid nitrogen, differentiate liquid nitrogen horizontal with platform scale, that takes out except these needs freezes except essence, and other freeze essence can not mention on below tank mouth 3.5cm line, when searching froze essence more than 10 seconds, barrelling will be divided to put back in liquid nitrogen, and then mention searching, to keep the cold degree freezing essence; Take out rearmounted 38 ~ 40 DEG C of water-baths, 5 seconds are instantaneous thaws; Each bull semen should be placed in same point of barrelling, and has subpackage inventory, inventory comprises bull number, quantity and takes record; When cleaning liquid nitrogen container, will freeze essence and be quickly transferred to the clean liquid nitrogen container of ready placement arranged side by side, freezing the essence exposed time outside tank can not more than 5 second.
(3) cow is joined in strict screening
Primary election growing cattle;
Primary election growing cattle is at 16 ~ 18 monthly ages, ginseng is joined cow and is selected excellent high yield cow and offspring thereof as use object, body weight reaches more than 350kg, nutrition and being in a good state of health, the growth of livestock is moderate, physically fit, without reproductive capability disease, for cervix uteri, the comparatively thin or hard person of cervix uteri does not select, and conception rate can reach more than 70%, can increase substantially Cow ratio and economic benefit within a short period of time;
Select multiparous cow;
Select healthy, without reproductive disease, without hard to settle history, without difficult labour history, without placenta retention history and other relevant diseases, more than 50 days puerperal, normal high yield cow of oestrusing, multiparity cattle conception rate is only about 30%-50%, and economic benefit is relatively low;
(4) breeding semen deposition
Outside observation finds that cow accepted mounting or other cattle of mounting after 12 ~ 16 hours, when per rectum checks that all are normal in uterus, follicular development is ripe or cow quietly to stand, accept mounting and vaginal orifice and flow out transparent input seminal fluid when there is strong stringiness mucus; Strong stringiness mucus, haircuts lift reach 6 ~ 8 times, draw 3 ~ 5 centimeter length to be semen deposition window of opportunitys.The method of described breeding semen deposition comprises the steps:
The sterilization of cow pudendum is carried out before A, semen deposition, drying and the health (thin film protective jacket should be used outside spermatic gun) of the clean and semen deposition utensil around cattle vaginal orifice is kept in semen deposition, spermatic gun uses thin film protective jacket outward, avoid wound as far as possible, use harmless to sperm, non-stimulated to reproductive tract lubricant;
B, outside observational method carry out detection of oestrus in conjunction with examination per rectum method, outside observational method, find that cow accepted mounting or other cattle of mounting after 12 ~ 16 hours, carry out examination per rectum, during examination per rectum, maneuver wants light, ovary is not easily affected to cause follicle to damage, touching follicle maturity state. follicular development is ripe, namely follicle is given prominence to, round and smooth, fluctuation obviously, have one touch namely broken sense after 1 ~ 2 hour or cow quietly to stand, accept mounting and vaginal orifice outflow is transparent when having strong stringiness mucus, for the estrus of cow phase, Inseminated time;
C, to estrus cow input seminal fluid, after during input seminal fluid, seme-deposition device upwards enters vaginal orifice vestibule section separately with 35 ~ miter angle degree, slightly forward downward enters vagina cervix uteri section; The whole gesture holding cervix uteri is soft, before seme-deposition device enters cervix uteri, cervix uteri can be leaned against on pelvis limit, and the vaginal wall extruded gently around cervix uteri, seme-deposition device is made to enter cervical orifice, the injection position of seminal fluid is the joint portion of body of uterus and cornua uteri, is determining to inject the errorless rear injection seminal fluid in position, seminal fluid can be injected the large turn of bilge of cornua uteri of ovulation side.
D, completing first service, when touching the ovulation of follicle Delay in Diagnosis, needing according to the method described above through the semen deposition of row second time.
The cow of being joined that the row breeding that progresses greatly is frozen in the control of employing property selects growing cattle, and adopts the semen deposition mode of, is most preferred embodiment.Xintai City Animal Husbandry and Veterinary Bureau applies Ox Biological Tech. Co., Ltd., Shandong's breeding oxen property control in the good and cattle farm of Zhai Zhen freezes essence, carries out breeding and tests.Date of test, from August, 2012, continues nearly 2 years.Now performance analysis is reported as follows:
One, property control frozen semen efficiency analysis
Result of the test statistics is as table 1:
Table 1: smart conception rate statistical table is frozen in the property control only of different cattle
From total non-return rate, the conception rate of growing cattle is 39%, and the conception rate of Grown cow is 25%.Growing cattle exceeds Grown cow conception rate 14%, and it is more suitable that the row breeding that progresses greatly is frozen in the control of visible growing cattle usability.
The non-return rate that essence is frozen in the control of yearling heifer usability on average can reach 39%, in actual production, have promotional value.With at present often the control of propping up property freeze essence 280 yuan of calculating. be then folded under different semen deposition mode and on average freeze smart expense in table 2 in every calf:
Table 2: the different semen deposition modes of growing cattle every calf freezes smart cost
As can be seen here, the row breeding that progresses greatly is frozen for usability control, while smart cost and breeding effect are frozen in consideration, yearling heifer should be selected as far as possible, adopt the semen deposition mode economic benefit of " 1 time 1 " maximum.
Two, property control frozen semen comprehensive benefit analysis (carrying out rough calculation with 100 adaptive yearling heifer scales)
(1) common value calculation of freezing essence is used
If use common frozen semen, often propping up price is about 40 yuan, and the average conception rate by 70% calculates, and the seminal fluid expense needed for that every calf is 57 yuan.
The natality of female calf calculates by 50%, every price 4000 yuan, then its values is: 50 × 4000=200000 is first;
The natality of bull calf is pressed 5O% and is calculated, every price 300 yuan, then its values is: 50 × 300=15000 is first;
Be worth and add up to: 200000+15000-100 × 57=209300 unit
Use that common to freeze the value cost of dishing out that essence produces be about 209300 yuan.
(2) value calculation of essence is frozen in usability control
If usability control frozen semen, often prop up price 280 yuan, the average conception rate by 39% calculates, and the seminal fluid expense so needed for every calf is then 717 yuan.And the natality of female calf can be made to bring up to nearly 90% with property control frozen semen.
The natality of female calf calculates according to 90%, every price 4000 yuan, and its values is: 90 × 4000=360000 is first;
The natality of bull calf calculates by 10%, every price 300 yuan, and its values is: lO × 300=3000 is first;
Be worth and add up to: 360000+3000-100 × 717=291300 unit
It is about 291300 yuan that the value cost of dishing out that essence produces is frozen in usability control
(3) performance analysis:
Yearling heifer usability control frozen semen owing to substantially increasing female calf natality, thus adds economic benefit, carries out theoretical calculate with 100 adaptive yearling heifer scales, and it is 82000 yuan that usability control freezes essence than common economic benefit of freezing essence increase.
As can be seen from above rough calculation, suppose that the scale of 100 yearling heifers is popularized usability control in an all-round way and frozen essence in breeding, only this item just can increase economic efficiency nearly 100,000 yuan, and namely every yearling heifer on average can increase the income of about 1000 yuan, and economic benefit is considerable.The method is simple to operation, usability control frozen semen substantially increases the natality of female calf, the natality of female calf brings up to nearly 90%, greatly shorten time milch cow generation inteval, milch cow reproductive speed is accelerated, the integral production performance of milch cow is improved, reduces cost, add economic benefit, have a good application prospect.
Embodiment described above is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Claims (5)
1. smart test-tube method is frozen in the control of milk obstinacy, it is characterized in that, comprises the following steps:
(1) sperm of high-quality bull containing X chromosome is chosen
Choose bull and reach more than 0.4 containing the motility of sperm of X chromosome, every agent is frozen smart rectilinear motion sperm quantity and is reached more than 2,200,000, and avirulent bacteria number is lower than 800/ml;
(2) seminal fluid is stored
To the Sperm storage of high-quality bull X chromosome be chosen in the liquid nitrogen container that fine vacuum is incubated, and liquid nitrogen container is placed on wooden backing plate that is clean, dry, that ventilate;
(3) cow is joined in strict screening
Primary election growing cattle;
Primary election growing cattle is at 16 ~ 18 monthly ages, and ginseng is joined cow and selected excellent high yield cow and offspring thereof as use object, and body weight reaches more than 350kg, nutrition and being in a good state of health, and the growth of livestock is moderate, physically fit, without reproductive capability disease;
Select multiparous cow;
Select healthy, without reproductive disease, without hard to settle history, without difficult labour history, without placenta retention history relevant disease, more than 50 days puerperal, normal high yield cow of oestrusing;
(4) breeding semen deposition
Outside observation finds that cow accepted mounting or other cattle of mounting after 12 ~ 16 hours, when per rectum checks that all are normal in uterus, follicular development is ripe or cow quietly to stand, accept mounting and vaginal orifice and flow out transparent input seminal fluid when there is strong stringiness mucus.
2. smart test-tube method is frozen in milk obstinacy control as claimed in claim 1, it is characterized in that, described step (2) stores seminal fluid as follows: the liquid nitrogen horizontal face in liquid nitrogen container should remain on more than 20cm, when being low to moderate 18cm, timely interpolation liquid nitrogen, liquid nitrogen horizontal is differentiated with platform scale, that takes out except these needs freezes except essence, other freeze essence can not mention on below tank mouth 3.5cm line, when searching froze essence more than 10 seconds, barrelling will be divided to put back in liquid nitrogen, and then mention searching, to keep the cold degree freezing essence; Take out rearmounted 38 ~ 40 DEG C of water-baths, 5 seconds are instantaneous thaws; Each bull semen should be placed in same point of barrelling, and has subpackage inventory, inventory comprises bull number, quantity and takes record; When cleaning liquid nitrogen container, will freeze essence and be quickly transferred to the clean liquid nitrogen container of ready placement arranged side by side, freezing the essence exposed time outside tank can not more than 5 second.
3. smart test-tube method is frozen in milk obstinacy control as claimed in claim 1, it is characterized in that, the strong stringiness mucus of described step (4), haircuts lift reach 6 ~ 8 times, draw 3 ~ 5 centimeter length to be semen deposition window of opportunitys.
4. smart test-tube method is frozen in milk obstinacy control as claimed in claim 1, it is characterized in that, the method for described step (4) breeding semen deposition comprises the steps:
Carry out the sterilization of cow pudendum before A, semen deposition, keep drying and the health of the clean and semen deposition utensil around cattle vaginal orifice in semen deposition, spermatic gun uses thin film protective jacket outward, avoids wound as far as possible, uses harmless to sperm, non-stimulated to reproductive tract lubricant;
B, outside observational method carry out detection of oestrus in conjunction with examination per rectum method, outside observational method, find that cow accepted mounting or other cattle of mounting after 12 ~ 16 hours, carry out examination per rectum, during examination per rectum, maneuver wants light, ovary is not easily affected to cause follicle to damage, touching follicle maturity state. follicular development is ripe, namely follicle is given prominence to, round and smooth, fluctuation obviously, have one touch namely broken sense after 1 ~ 2 hour or cow quietly to stand, accept mounting and vaginal orifice outflow is transparent when having strong stringiness mucus, for the estrus of cow phase, Inseminated time;
C, to estrus cow input seminal fluid, after during input seminal fluid, seme-deposition device upwards enters vaginal orifice vestibule section separately with 35 ~ miter angle degree, slightly forward downward enters vagina cervix uteri section; The whole gesture holding cervix uteri is soft, before seme-deposition device enters cervix uteri, cervix uteri can be leaned against on pelvis limit, and the vaginal wall extruded gently around cervix uteri, seme-deposition device is made to enter cervical orifice, the injection position of seminal fluid is the joint portion of body of uterus and cornua uteri, is determining to inject the errorless rear injection seminal fluid in position, seminal fluid can be injected the large turn of bilge of cornua uteri of ovulation side.
D, completing first service, when touching the ovulation of follicle Delay in Diagnosis, needing according to the method described above through the semen deposition of row second time.
5. smart test-tube method is frozen in the milk obstinacy control as described in as arbitrary in claim 1-4, it is characterized in that, the cow of being joined that the row breeding that progresses greatly is frozen in the control of employing property selects growing cattle, and adopts the semen deposition mode of, is most preferred embodiment.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758084A (en) * | 2015-04-20 | 2015-07-08 | 陈绪凯 | Method for artificial insemination for cow through sex control and semen freezing |
| CN107157612A (en) * | 2017-03-29 | 2017-09-15 | 吴学权 | It is a kind of to improve the method that frozen cattle semens transmission ＆ distribution award tire rate |
| CN108782436A (en) * | 2018-05-23 | 2018-11-13 | 澜沧宝来农牧产业有限公司 | A method of it is horizontal to improve Yunnan peak Niu Fanyu |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758084A (en) * | 2015-04-20 | 2015-07-08 | 陈绪凯 | Method for artificial insemination for cow through sex control and semen freezing |
| CN107157612A (en) * | 2017-03-29 | 2017-09-15 | 吴学权 | It is a kind of to improve the method that frozen cattle semens transmission ＆ distribution award tire rate |
| CN108782436A (en) * | 2018-05-23 | 2018-11-13 | 澜沧宝来农牧产业有限公司 | A method of it is horizontal to improve Yunnan peak Niu Fanyu |
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Application publication date: 20150624 |