CN110521939A - A kind of probiotics fermention food and preparation method thereof adjusting intestinal health - Google Patents

A kind of probiotics fermention food and preparation method thereof adjusting intestinal health Download PDF

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Publication number
CN110521939A
CN110521939A CN201910922333.2A CN201910922333A CN110521939A CN 110521939 A CN110521939 A CN 110521939A CN 201910922333 A CN201910922333 A CN 201910922333A CN 110521939 A CN110521939 A CN 110521939A
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lactobacillus
fermentation
probiotics
bifidobacterium
cfu
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张震
王海宽
梁武
李树森
王飞
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Tianjin Inncorigin Biological Technology Co ltd
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Tianjin Inncorigin Biological Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
    • A23L11/07—Soya beans, e.g. oil-extracted soya bean flakes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50—Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/173—Reuteri
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/41—Pediococcus
    • A23V2400/413—Acidilactici
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/513—Adolescentes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/515—Animalis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

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Abstract

The invention belongs to microbial fermentation application fields, and in particular to a kind of functional food and preparation method thereof by the segmentation solid state fermentation soya bean production of a variety of probiotics.The probiotics fermention functional food provided by the present invention for adjusting intestinal health is will to mix access probiotics after soybean powder with water in proportion to carry out segmentation solid state fermentation and obtain.The probiotics includes lactobacillus reuteri, bifidobacterium longum, Pediococcus acidilactici, lactobacillus plantarum, animal bifidobacteria, lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis.By probiotics fermention, increase the benefit active in soybean, and keep it more easily digested, enhance its health-care efficacy to body, the health of body can also be adjusted and be played a significant role.

Description

A kind of probiotics fermention food and preparation method thereof adjusting intestinal health
Technical field:
The invention belongs to microbial fermentation application fields, and in particular to a kind of to be segmented solid state fermentation soya bean by a variety of probiotics Functional food of production and preparation method thereof.
Background technique:
There are close relationship, the occurrence and development of some diseases are usually associated with intestinal flora for intestinal flora and human health Variation.In addition, enteron aisle or the maximum immune organ of human body, Intestinal Mucosal Immunity system include gut-associated lymphoid tissue and Related cell, molecular chaperones, it can resist bacterium, virus and toxin and invade from alimentary canal, be responsible for important immune function, It is to prevent the intracorporal firm barrier together of extraneous virulence factor invasion.But with current people's the accelerating rhythm of life, diet is practised The used variation with environment, the environment such as many people live in disease, fatigue, pressure is excessive, abuse of antibiotics, has not enough sleep, drinks In, so that different degrees of intestinal flora occur unbalance by quite a few crowd, and then influence health.Data show, I The number of state stomach patient is up to 2.3 hundred million people, wherein there are about 40% patients for a long time by the puzzlement of gastrointestinal disease, PATIENTS WITH LARGE BOWEL It is in rising trend.In addition, there are different degrees of upset,gastro-intestinals by the resident in China about 70%；Occur per year over 1000000000 person-times Diarrhea or constipation symptom increase PATIENTS WITH LARGE BOWEL newly up to 400,000.Moreover, intestinal flora is unbalance can also seriously affect exempting from for body Epidemic disease systematic function, causes immunologic hypofunction, and then causes a variety of diseases, as cirrhosis, gall stone, coronary heart disease, myocardial infarction, Cerebral arteriovenous malformation etc..It is much for the product of intestinal health problem exploitation currently on the market, it mainly include more traditional Traditional Chinese and western medicine drug and the probiotic products risen in recent years, but the former often adaptability is low, Special medicine for special disease, and majority has secondary work With the functional food that cannot function as maintenance intestinal health is taken for a long time, can only take when something goes wrong in enteron aisle, limitation Greatly.And probiotic products were quickly grown in recent years, probiotics is referred to as microbial food replenishers living, by improving enteron aisle Microbial balance, to influence host health.Have some patents that intestinal health is adjusted using probiotics at present:
Publication number: the invention of CN106820155A is " for adjusting the lactic bacteria composition of intestinal flora and strengthen immunity And preparation method thereof " disclose it is a kind of for adjusting the lactic bacteria composition of intestinal flora and strengthen immunity, including lactic acid bacteria Group synkaryon, the lactic acid bacteria group synkaryon are wrapped with coated layer: the coated layer includes the composition of parts by weight: oligomeric different malt Sugared 30-50, protein substance 30-50, nitrocellulose 5-10, organic acid rare-earth salts 0.5-1；The lactic acid bacteria group synkaryon is by such as The bacterial strain of lower parts by weight forms: bifidobacterium lactis 30-40, lactobacillus reuteri 20-30, Lactobacillus rhamnosus 10-20, baby Bifidobacterium 10-20.
Publication number: a kind of invention " probiotic composition for adjusting intestinal flora health " of CN106820156A discloses one Kind includes the synthesis probiotic products of lactoferrin, inulin, bromelain, Radish extract, is rich in acidophilus in the product Lactobacillus, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium bifidum, Lactobacillus rhamnosus, Bulgaria Add in the beneficial bacteria of intestinal tract such as lactobacillus, lactobacillus plantarum, Lactobacillus casei, lactobacillus paracasei, streptococcus thermophilus and the present invention The substances such as lactoferrin, inulin and bromelain, Radish extract are entered.
Publication number: the invention " adjusting intestinal flora and the ferment of bowel relaxing functions and preparation method thereof " of CN107125745A is public A kind of adjusting intestinal flora and the ferment of bowel relaxing functions and preparation method thereof, including following raw material and compound microorganism ferments are opened It is prepared: fruit 75-82wt%, draft medicinal material 10-14wt% and ingredient 6-10wt%, the addition of the complex microorganism Amount is the 10-14wt% of the total weight of the raw material；The fruit is the mixture of pineapple, pawpaw, tomato and carrot, described Draft medicinal material is wheat, oat and the mixture of aloe, and the ingredient is inulin, stachyose, milk ketose, iMOS With the mixture of oligofructose, the complex microorganism is bifidobacterium bifidum, bifidobacterium longum, bifidobacterium adolescentis, acidophilus The Mixed Microbes or bifidobacterium bifidum of lactobacillus and streptococcus thermophilus, bifidobacterium longum, bifidobacterium adolescentis, lactobacillus fermenti With the Mixed Microbes of Lactobacillus helveticus.
The above-mentioned patent that adjusting enteral product is produced using probiotics fermention is mostly prebiotic strains liquid fermentation, and is mostly primary Fermented product, each growth is horizontal single or uneven, and raw material must be extracted with finished product, concentration, centrifugation and etc., to benefit The destruction of raw bacterium entirety microenvironment is big, and earlier fermentation and late stage process are complicated and waste material is more, and active ingredient loss is big, product vulnerable to Pollution, and more auxiliary material is added, product not can guarantee the adaptability and product effect of different crowd.And it is carried out using probiotics It is segmented solid state fermentation and production process no waste mine, beneficial activity ingredient free of losses, the probiotics of each growth of probiotics horizontal equilibrium Fermented food and preparation method thereof has not been reported.
Summary of the invention:
The present invention is directed to and mention in view of the shortcomings of the prior art, provide a kind of probiotics fermention food for adjusting intestinal health For the preparation method of the food, especially for having a digestive problems and enteric flora disturbance, the crowd of hypoimmunity, and Research Thinking is provided for the production of Related product.
The present invention is segmented solid state fermentation soybean by probiotics to produce a kind of adjusting intestinal flora balance, maintains enteron aisle strong Health enhances the functional food of immunity of organisms.For soybean substrate, a kind of segmentation process for solid state fermentation has been designed and developed, it will Different probiotics are accessed in soybean at times by its growth rhythm in soybean ferments, so that each strain quantity in tunning Balance, metabolite content is abundant, and probiotics quantity is high, and finally obtains one kind and disappear with intestinal flora balance, improvement is adjusted Change system Tiny ecosystem maintains intestinal health, enhances the probiotics functionality fermented food of immunity of organisms.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of probiotics fermention functional food adjusting intestinal health is will to mix to access with water in proportion after soybean powder Probiotics carries out segmentation solid state fermentation and obtains.
The probiotics includes lactobacillus reuteri, bifidobacterium longum, Pediococcus acidilactici, lactobacillus plantarum, animal bifid Bacillus, lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis.
The fineness of the soybean powder is preferably to be not less than 100 mesh.
The ratio of the soy meal and water is preferably 1: 1.2-1: 2.0 (mass volume ratios).
The present invention provides the preparation method of the probiotics fermention functional food of above-mentioned adjusting intestinal health, and specific steps are such as Under:
(1) washing soybean is placed on 60 DEG C of baking ovens to dry 5-6 hours, milling sieves with 100 mesh sieve, and obtains soy meal；
(2) it prepares activation medium: adding 12g soy meal in every 100ml water, obtain activation medium；To activation medium It sterilizes, condition is 121 DEG C, 20min；
(3) configure fermentation medium: taking soy meal: water=1: 1.2-1: 2.0 (mass volume ratios), mixing are placed on solid Sterilizing in state fermentor, it is spare that solid-state fermentation culture medium is made；Sterilising conditions are 121 DEG C, 20min；
(4) actication of culture: lactobacillus reuteri, bifidobacterium longum, Pediococcus acidilactici, lactobacillus plantarum, animal bifid bar Bacterium, lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis glycerol tube press 1% (v/v respectively The volume ratio of activation medium) it is inoculated in activation medium and is activated, pH is naturally, controlled at 37 DEG C；It activates respectively for 24 hours Afterwards, by 5% (v/v) connect bacterium amount transfer again activation medium carry out re-activation, 37 DEG C reactivation for 24 hours afterwards secondary seed Liquid；
(5) probiotics stepwise fermentation:
1. in the bacillus subtilis that activation is completed and bacillus coagulans access solid-state fermenter, making every gram of fermentation training The inoculum concentration for supporting bacillus subtilis in base is 105-106CFU/g, the inoculum concentration of bacillus coagulans are 106-107CFU/g, dimension 37 DEG C of temperature are held, ventilatory capacity 0.1-0.5m3/ min, ferment 10-18h；
2. 1. being walked after fermentation to, activated animal bifidobacteria, Pediococcus acidilactici and lactobacillus plantarum are connect Enter in solid-state fermenter, makes the inoculum concentration 10 of B. animais in every gram of fermentation medium6-107CFU/g, Pediococcus acidilactici Inoculum concentration be 106-107CFU/g, the inoculum concentration of lactobacillus plantarum are 105-106CFU/g maintains 37 DEG C of temperature, sealed fermenting 12-24h；
3. 2. being walked after fermentation to, by activated bifidobacterium adolescentis, long Bifidobacterium Bifidum and lactobacillus paracasei It accesses in solid-state fermenter, makes the inoculum concentration 10 of bifidobacterium adolescentis in every gram of fermentation medium5-106CFU/g, long double qi bars The inoculum concentration of bacterium is 106-107CFU/g, the inoculum concentration of lactobacillus paracasei are 106-108CFU/g, 37 DEG C of temperature of maintenance are closed Ferment 18-36h；
4. 3. being walked after fermentation to the, activated lactobacillus reuteri is accessed in solid-state fermenter, makes every gram of hair The inoculum concentration of lactobacillus reuteri is 10 in ferment culture medium7-108CFU/g maintains 37 DEG C of temperature, sealed fermenting 24-48h；
(6) after fermentation, it is put into vacuum freeze drier and 24-35h is lyophilized, the probiotics for adjusting intestinal health is made Fermentation Function food；
The soybean is commercially available Non-transgenic soybean；
Preferably, the soybean: water=1: 1.5 (mass volume ratios)；
Preferably, after solid state fermentation, vacuum freeze drying 30h is carried out, until water content is not more than 10%；
Preferably, the lactobacillus plantarum be lactobacillus plantarum IOB602 (Lactobacillus plantarum), in On June 29th, 2018 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: Beijing's southern exposure No. 3 Institute of Microorganism, Academia Sinica, institute of area North Star West Road 1, postcode 100101, deposit number are CGMCC No.16021；
Preferably, the animal bifidobacteria is animal bifidobacteria IOB402 (Bifidobacterium Animalis), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 29th, 2018, Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode 100101, deposit number are CGMCC No.16028；
Preferably, the lactobacillus paracasei is lactobacillus paracasei IOB413 (Lactobacillus paracasei), It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 29th, 2018, address: Beijing The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.16022；
Preferably, the bifidobacterium adolescentis is bifidobacterium adolescentis IOB506 (Bifidobacteriumadolescentis), Chinese microorganism strain preservation management committee is preserved on June 29th, 2018 Member's meeting common micro-organisms center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, Postcode 100101, deposit number are CGMCC No.16026；
Preferably, the bacillus coagulans be bacillus coagulans IOB502 (Bacillus coagulans), in On June 29th, 2018 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows: court, Beijing The institute 3 of positive area's North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number are as follows: CGMCC No.16025；
Preferably, the bacillus subtilis be bacillus subtilis IOB430 (Bacillus subtilis), in On June 29th, 2018 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows: court, Beijing The institute 3 of positive area's North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.16024；
Preferably, the lactobacillus reuteri is lactobacillus reuteri IOB423 (Lactobacillus reuteri), China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are preserved on June 29th, 2018 are as follows: Beijing The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.16023；
Preferably, the bifidobacterium longum be bifidobacterium longum IOB515 (Bifidobacterium longum), in On June 29th, 2018 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows: court, Beijing The institute 3 of positive area's North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.16027；
Preferably, the Pediococcus acidilactici is Pediococcus acidilactici IOB701 (Pediococcus acidilactici), China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are preserved on July 10th, 2018 are as follows: Beijing The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.16077。
The utility model has the advantages that
Probiotics is a kind of by improving intestine microenvironment to the microorganism to host's generation beneficial effect.It can Inhibit growth of pathogenic bacteria by participating in intestinal microecology balance, maintains intestinal health.A large number of studies show that probiotics can be adjusted It saves body gastrointestinal tract normal flora, keep microecological balance, improve food digestion rate and biological value, reduce serum cholesterol, control Endotoxin processed inhibits the growth and breeding of spoilage organisms and the generation of spoilage product in enteron aisle, manufactures nutriment, stimulation group hair-weaving It educates, to send out the nutritional status of body, physiological function, cell infection, drug effect, toxic reaction, immune response, tumour The generations effect such as raw, aging course and unexpected emergency reaction.
For soybean originating from China, China is that soy food product is original earliest, spreads country at most, just there is human consumption soybean very early Tradition of fermented product, including fermented soya bean, fermented bean drink, yellow bean sauce and various fermented bean curd etc. are all with soybean or bean product inoculation mould hair Made of after ferment.The nutritive value of soybean is very high, referred to as " king in beans ", " meat of Tanaka ", " green cow's milk " etc., is several The food most praised highly by nutritionist in hundred kinds of natural foods.Soybean protein rich in, isoflavones, plant are solid Alcohol, soyabean oligosaccharides etc. can enhance immunity of organism, prevent vascular sclerosis, have the effects that hypoglycemic, anti-oxidant, furthermore soybean It is also rich in dietary fiber, and dietary fiber is very important to promoting good digestion and excretion solid waste to play the role of.It grinds Study carefully and show appropriately supplementary fibre element, the food increase in enteron aisle can be made to soften, promote intestines peristalsis, to accelerate defecation speed Degree prevents constipation and reduces the risk of intestinal cancer.Meanwhile dietary fiber has apparent reduction plasma cholesterol, adjusts stomach and intestine function The functions such as energy and insulin level.
The probiotics fermention functional food provided by the invention for adjusting intestinal health, had both saved the nutriture value of natural plants Value, but the nutrition health-care functions with probiotics fermention product, effect are obvious；The probiotics fermention can adjust intestinal health Food by be segmented solid state fermentation be prepared, by probiotics fermention, increase the benefit active in soybean, and make It is more easily digested, enhances its health-care efficacy to body.The probiotic food of this patent, which removes, has intestinal health Have outside splendid health-care effect, the health of body can also be adjusted and be played a significant role.
Specific embodiment:
The technical solution of the present invention will be described in detail below with reference to the embodiments and experimental examples, specific embodiment described herein Be only used to explain the present invention, but the present invention is not limited to following specific embodiments and protection scope it is without being limited thereto.
The percentage sign " % " being related in embodiment refers to mass percent if not specified, and the percentage of solution refers to Containing the grams of solute in 100mL, percentage between liquid refers to the volume ratio of the solution at 25 DEG C.
Bacterial strain employed in following embodiment is lactobacillus plantarum IOB602 (Lactobacillus plantarum), Deposit number is CGMCC No.16021；Animal bifidobacteria IOB402 (Bifidobacterium animalis), preservation are compiled Number be CGMCC No.16028；Lactobacillus paracasei IOB413 (Lactobacillus paracasei), deposit number is CGMCC No.16022；Bifidobacterium adolescentis IOB506 (Bifidobacteriumadolescentis), deposit number are CGMCC No.16026；Bacillus coagulans IOB502 (Bacillus coagulans), deposit number are as follows: CGMCC No.16025；Bacillus subtilis IOB430 (Bacillus subtilis), deposit number are CGMCC No.16024；Roy Family name's lactobacillus IOB423 (Lactobacillus reuteri), deposit number are CGMCC No.16023；Bifidobacterium longum IOB515 (Bifidobacterium longum), deposit number are CGMCC No.16027；Pediococcus acidilactici IOB701 (Pediococcus acidilactici), deposit number are CGMCC No.16077.
Actication of culture mode used by following embodiment is as follows if not otherwise specified:
(1) it prepares activation medium: adding 12g soy meal in every 100ml water, obtain activation medium；To activation medium It sterilizes, condition is 121 DEG C, 20min；
(2) actication of culture: lactobacillus reuteri, bifidobacterium longum, Pediococcus acidilactici, lactobacillus plantarum, animal bifid bar Bacterium, lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis glycerol tube press 1% (v/v respectively With the volume ratio of activation medium) it is inoculated in activation medium and is activated, pH is naturally, controlled at 37 DEG C；It activates respectively After for 24 hours, by the bacterium amount that connects of 5% (v/v), switching activation medium carries out re-activation respectively again, and 37 DEG C of reactivations obtain afterwards for 24 hours Secondary seed solution.
The present invention will be explained by specific embodiment below.
Embodiment 1: the probiotics fermention preparation method of functional food of intestinal health is adjusted
1, the determination of probiotics inoculum concentration
(1) washing soybean is placed on 60 DEG C of baking ovens to dry 5-6 hours, milling sieves with 100 mesh sieve, and obtains soy meal；
(2) it prepares activation medium: adding 12g soy meal in every 100ml water, obtain activation medium；To activation medium It sterilizes, condition is 121 DEG C, 20min；
(3) configure fermentation medium: taking soy meal: water=1: 1.4 (mass volume ratios), mixing are placed on 6T solid state fermentation Sterilizing in tank, it is spare that solid-state fermentation culture medium is made；6T solid-state fermenter sterilising conditions are 121 DEG C, 20min；
(4) actication of culture: lactobacillus reuteri, bifidobacterium longum, Pediococcus acidilactici, lactobacillus plantarum, animal bifid bar Bacterium, lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis glycerol tube press 1% (v/v respectively The volume ratio of activation medium) it is inoculated in activation medium and is activated, pH is naturally, controlled at 37 DEG C；It activates respectively for 24 hours Afterwards, by 5% (volume ratio of v/v re-activation culture medium) connect bacterium amount transfer again activation medium carry out re-activation, 37 DEG C reactivation for 24 hours afterwards secondary seed solution；
(5) probiotics fermention:
1. the bacillus subtilis that activation is completed and bacillus coagulans access 6T solid-state fermenter by inoculation pipeline In, 37 DEG C of temperature are maintained, ventilatory capacity 0.1m3/ min, ferment 10h；
2. 1. being walked after fermentation to, activated animal bifidobacteria, Pediococcus acidilactici and lactobacillus plantarum are led to It crosses in inoculation pipeline access 6T solid-state fermenter, maintains 37 DEG C of temperature, sealed fermenting 12h；
3. 2. being walked after fermentation to, by activated bifidobacterium adolescentis, long Bifidobacterium Bifidum and lactobacillus paracasei It is accessed in 6T solid-state fermenter by inoculation pipeline, maintains 37 DEG C of temperature, sealed fermenting 18h；
4. 3. walking after fermentation to the, activated lactobacillus reuteri is accessed into 6T solid state fermentation by inoculation pipeline In tank, 37 DEG C of temperature are maintained, sealed fermenting is for 24 hours；
(6) each strain inoculum concentration is respectively connected in following ratio, after fermentation, respectively to the probiotics in fermented sample Viable count is detected, as a result such as table 1:
Strain label: No. 1 is lactobacillus plantarum single bacterium, and No. 2 are animal bifidobacteria single bacterium, and No. 3 are lactobacillus paracasei Single bacterium, No. 4 are bifidobacterium adolescentis single bacterium, and No. 5 are bacillus coagulans single bacterium, and No. 6 are lactobacillus reuteri single bacterium, and No. 7 are Bifidobacterium longum single bacterium, No. 8 are Pediococcus acidilactici single bacterium, and No. 9 are bacillus subtilis single bacterium；
Inoculum concentration: the inoculum concentration of bacillus subtilis is fixed as 5 × 105CFU/g, bacillus subtilis, condensation gemma Bacillus, bifidobacterium longum, Pediococcus acidilactici, lactobacillus plantarum, animal bifidobacteria, lactobacillus paracasei, bifidobacterium adolescentis, Lactobacillus reuteri accesses in solid-state fermentation substrate in proportion in batches, bacillus subtilis, bacillus coagulans, animal bifid Bacillus, Pediococcus acidilactici, lactobacillus plantarum, bifidobacterium adolescentis, bifidobacterium longum, lactobacillus paracasei, lactobacillus reuteri The ratio between inoculum concentration are as follows:
Group unification: 1: 10: 10: 10: 1: 1: 10: 50: 100；
Combination two: 1: 5: 5: 10: 1: 1: 10: 100: 100；
Combination three: 1: 10: 5: 10: 1: 1: 10: 50: 100；
Combination four: 1: 5: 10: 5: 1: 1: 10: 50: 100；
Combination five: 1: 10: 5: 10: 1: 1: 10: 100: 100；
Combination six: 1: 5: 10: 10: 1: 1: 5: 50: 100；
Combination seven: 1: 10: 5: 20: 1: 1: 10: 50: 100；
Combination eight: 1: 10: 10: 10: 1: 1: 10: 100: 100；
Combination nine: 1: 10: 10: 5: 1: 1: 10: 100: 100.
Number of live bacteria of probiotics (CFU/g) after each strain different vaccination amount solid state fermentation of table 1
Group unification Combination two Combination three Combination four Combination five Combination six Combination seven Combination eight Combination nine
No. 1 2.77×109 9.02×108 4.23×108 6.65×108 5.12×109 3.45×108 5.34×108 4.82×109 7.56×108
No. 2 4.55×109 3.75×109 7.84×107 8.76×107 7.67×109 2.94×109 6.56×108 4.67×109 3.13×108
No. 3 6.99×109 1.02×108 9.87×107 4.76×108 7.32×108 5.34×108 4.01×108 3.17×109 2.19×109
No. 4 1.63×107 8.27×107 5.75×108 7.88×108 9.65×108 6.32×108 3.69×108 8.16×108 3.65×108
No. 5 2.01×107 4.25×107 5.26×107 7.48×107 5.05×109 5.25×108 4.15×108 3.19×109 1.86×108
No. 6 3.98×108 8.01×108 9.66×107 3.26×107 5.74×107 7.93×107 3.16×107 4.27×109 3.79×107
No. 7 1.05×109 1.43×108 2.34×108 4.32×108 3.28×109 5.12×108 2.17×109 5.32×109 6.32×108
No. 8 2.28×107 4.68×107 7.47×107 1.78×108 8.36×107 9.78×107 6.68×107 7.34×108 8.43×107
No. 9 3.43×109 1.57×107 6.19×107 2.18×108 7.27×107 8.12×107 5.15×107 6.45×108 7.46×107
Total viable count 2.17×1010 6.25×109 8.06×108 2.67×109 2.01×1010 7.85×109 8.13×109 4.04×1010 3.31×109
The ratio between optimal inoculum concentration obtained as shown in Table 1 is combination eight: 1: 10: 10: 10: 1: 1: 10: 100: 100, at this time Total viable count is 4.04 × 1010CFU/mL。
2, the determination of material-water ratio
The ratio (mass volume ratio) of soy meal in solid-state fermentation culture medium and water is made into 1:1.2,1:1.4,1 respectively: 1.6,1:1.8, the inoculum concentration of bacillus subtilis is 5 × 105CFU/g, bacillus subtilis, bacillus coagulans, animal are double Discrimination bacillus, Pediococcus acidilactici, lactobacillus plantarum, bifidobacterium adolescentis, bifidobacterium longum, lactobacillus paracasei, Luo Yishi cream bar The ratio between bacterium inoculum concentration is 1: 10: 10: 10: 1: 1: 10: 100: 100 inoculations, remaining step is fermented with embodiment 1-1, is fermented After, the number of live bacteria of probiotics in fermented sample is detected respectively, as a result such as table 2:
Viable count (CFU/g) after the fermentation substrate fermentation of the different material-water ratios of table 2
Ratio 1:1.2 1:1.4 1:1.6 1:1.8
Lactobacillus plantarum single bacterium 1.87×109 2.71×109 3.25×109 1.51×109
B. animais single bacterium 3.28×109 4.37×109 2.85×109 1.13×109
Lactobacillus paracasei single bacterium 4.39×108 5.11×108 3.28×108 2.58×108
Bifidobacterium adolescentis single bacterium 5.69×108 6.27×108 9.24×108 3.42×108
Bacillus coagulans single bacterium 1.24×109 3.15×109 3.76×109 1.50×109
Lactobacillus reuteri single bacterium 3.98×109 7.29×109 7.77×109 4.54×109
Long Bifidobacterium Bifidum single bacterium 5.78×108 7.47×108 6.32×108 7.21×108
Pediococcus acidilactici single bacterium 4.23×109 6.32×109 8.38×109 6.54×109
Bacillus subtilis single bacterium 2.97×109 3.26×109 4.19×109 3.12×109
Total viable count 2.61×1010 3.98×1010 4.53×1010 1.39×1010
The results are shown in Table 2: best material-water ratio is 1:1.6, that is, is measured total in gained probiotics fermention functional food Viable count is 4.53 × 1010CFU/g。
3, the determination of fermentation time
Each phases-time of probiotics fermention is identified as following groups:
Group 1:10h, 12h, 18h, for 24 hours；
Group 2:18h, for 24 hours, 36h, 48h；
Group 3:15h, 18h, for 24 hours, 36h；
Group 4:12h, 18h, for 24 hours, for 24 hours；
Group 5:18h, 18h, 36h, 36h.
The inoculum concentration of bacillus subtilis is 5 × 105CFU/g, bacillus subtilis, bacillus coagulans, animal bifid Bacillus, Pediococcus acidilactici, lactobacillus plantarum, bifidobacterium adolescentis, bifidobacterium longum, lactobacillus paracasei, lactobacillus reuteri The ratio between inoculum concentration is 1: 10: 10: 10: 1: 1: 10: 100: 100 inoculations, material-water ratio 1:1.6, remaining condition with embodiment 1-1, Count plate is carried out after fermentation, as a result such as table 3:
Viable count (CFU/g) when 3 different fermentations of table after indirect fermentation
Group 1 Group 2 Group 3 Group 4 Group 5
Lactobacillus plantarum single bacterium 3.12×109 5.19×109 3.77×109 3.94×109 3.81×109
B. animais single bacterium 3.01×109 4.29×109 2.21×109 1.36×109 8.15×108
Lactobacillus paracasei single bacterium 2.78×108 2.37×108 6.71×108 3.45×108 1.37×108
Bifidobacterium adolescentis single bacterium 7.03×108 6.47×108 5.17×108 5.49×108 5.42×108
Bacillus coagulans single bacterium 2.12×109 2.69×109 2.57×108 6.01×108 2.57×109
Lactobacillus reuteri single bacterium 6.25×109 6.51×109 3.92×108 7.41×108 3.98×109
Long Bifidobacterium Bifidum single bacterium 5.53×108 6.02×108 5.31×108 4.71×108 2.37×108
Pediococcus acidilactici single bacterium 7.55×109 7.17×109 6.88×109 2.54×109 5.78×109
Bacillus subtilis single bacterium 4.01×109 3.67×109 2.69×109 2.85×109 2.88×109
Total viable count 4.37×1010 4.86×1010 1.75×1010 2.54×1010 2.89×1010
The results are shown in Table 3: each stage Best Times of probiotics fermention combination two, be respectively as follows: 18h, for 24 hours, 36h, 48h, measuring total viable count in probiotics fermention functional food at this time is 4.86 × 1010CFU/g。
4, fermentation front and back culture medium active constituent variation
By above-mentioned optimum inoculation amount, best material-water ratio, best fermentation time, remaining is consolidated referring to embodiment 1-1 step State fermentation, compares before carrying out composition detection, with fermentation after fermentation.Utilize Syrups by HPLC gossypose, wood Sugared content；Utilize forint phenol reaction assay polypeptide and polyphenol content；Using GB5009.88-2014, " national food safety standard is eaten The measurement of dietary fiber in product " in method measurement dietary fiber content；With reference to article " the colorimetric method survey of Song Hongzhe, Zhao Yong etc. Determine the parsing of soyasaponin " measurement soyasaponin content；With reference to article " the spectrophotometry measurement of Liu Pengli, Zhang Shuanling etc. Phosphatidylcholine content in soybean lecithin " measuring method measurement soybean lecithin content；With reference to the article of Zheng Bin, Wang Shu etc. Method in " protein and protease content measurement in blueberry ferment " measures protease content；It is surveyed using the quick colorimetric of amylase Method is determined to measure the vigor of amylase；With reference in GBT22492-2008 " National Standard of the People's Republic of China's soy peptide powder " Method measures content of peptides；It is surveyed with reference to the method in noble, Han Si equality article " comparison of lipase activity detection method " Determine lipase activity；Utilize side in GB5009235-2016 " measurement of amino-acid nitrogen in national food safety standard food " Method one measures amino-acid state.
The culture medium active constituent variation of the fermentation of table 4 front and back
As shown in Table 4, after probiotics fermention, polyphenol, stachyose, gossypose, dietary fiber in solid-state fermentation substrate, Soyasaponin, soybean lecithin, protease, amylase, lipase, antibacterial peptide, amino-acid nitrogen content be significantly increased. Polyphenol can interact in human body with enteric microorganism, influence intestinal flora and then being degraded by enteric microorganism, change Kind intestinal microflora, increases the quantity of lactobacillus and Bifidobacterium, further influences human health；Dietary fiber is good to promoting Good digestion and excretion solid waste plays the role of very important.Studies have shown that appropriately supplementary fibre is plain, can make in enteron aisle Food increase soften, promote intestines peristalsis, to accelerate defecation speed, prevent constipation and reduce the risk of intestinal cancer；Study table Bright, soyasaponin has the function of adjusting immune；Protease, lipase, amylase can effectively promote as digestive ferment to disappear Change, reduce digestive system burden, promotes digestive system health；" food " of the stachyose as probiotics, in human gastrointestinal tract The profitable strains such as Bifidobacterium, Bacillus acidi lactici have extremely apparent proliferation function, environment in human body alimentary canal can be improved rapidly, Adjust Tiny ecosystem colony balance；Gossypose can promote simultaneously the flourish of the beneficial bacteriums such as Bifidobacterium, Bacillus acidi lactici, and have Effect inhibits the breeding of harmful intestinal tract bacteria, establishes the intestinal flora environment of health, in addition, gossypose belongs to water-soluble dietary fiber, Equivalent efficacy with dietary fiber；Polypeptide can directly be absorbed by intestinal cell, mitigate the burden of enteron aisle, in addition, polypeptide is also The beneficial bacteriums such as Bacillus acidi lactici, the Bifidobacterium in enteron aisle can be promoted to grow, promote enterogastric peristalsis and food absorption, to gastritis etc. Disease of digestive system has good preventive and therapeutic effect；Amino-acid nitrogen can react probiotics to the fermentation journey of substrate indirectly Degree, also proportional with the content of amino acid, amino acid nitrogen content increases, and illustrates the part macromolecular substances quilt in substrate It decomposes for small-molecule substance, it is easier to which absorption of human body reduces the burden to digestive system.
5, it is lyophilized
It by the fermentation medium of optimal fermentation condition output, is put into vacuum freeze drier and 30h is lyophilized, be made and adjust intestines The probiotics fermention functional food of road health.
Embodiment 2: improve the experimental study experimental method of intestinal microflora:
1, the preparation of the probiotics fermention food of intestinal health is adjusted: after preparing probiotics fermention food by embodiment 1-4 30h is lyophilized in vacuum freeze drier.
2, experimental animal: taking male, and weight 18-22g is separated from milk kunming mice 90 of one month, solves purchased from Chinese people Fang Jun The Fourth Military Medical University Experimental Animal Center.
3, experiment reagent: Bifidobacterium selective medium (BBL), Bifidobacterium selective culture medium (Lbs), Yihong methylene blue Agar, Sodium azide-crystal violet-aesculin agar, tryptone-sulphite-seromycin agar basis (TSC), D-Cycloserine Solution, physiological saline.
4, experimental procedure: being divided into blank group, experimental group 1, experimental group 2 for 90 mouse, aseptic collection each group before testing Stool in mice carries out count plate with 10 times of gradient dilutions respectively.Blank group is every with 0.10mL/10g mouse weight sterile saline 2 stomach-fillings of day, continuous 14d；Common probiotic food 10g in the market is added 20mL sterile distilled water to dilute by experimental group 1, with The weight dilution stomach-filling 2 times a day of 0.10mL/10g mouse, continuous 14d；Experimental group 2 adds probiotic food 10g produced by the invention 20mL sterile distilled water dilution, with the weight dilution stomach-filling 2 times a day of 0.10mL/10g mouse, continuous 14d；Last time gavages for 24 hours Aseptic collection each group stool in mice afterwards carries out count plate.Bifidobacterium is detected with BBL agar, detects newborn bar with Lbs agar Bacterium, detects enterobacteria with eosin methylene blue agar, detects enterococcus with Sodium azide-crystal violet-aesculin agar, is examined with TSC agar Survey C.perfringens.
5, mouse general state is observed: diet situation, the mobility, hair of mouse each group are observed being since first time stomach-filling Color, stool situation etc..
Experiment conclusion:
1, the general status of mouse: administration front and back, mouse is active active, sensitiveer to the reacting condition of ambient enviroment, And appetite is good.
2, Bifidobacterium, lactobacillus, enterobacteria, enterococcus, C.perfringens quantity in the mouse intestinal of administration front and back It influences, is shown in Table 5.
The administration front and back mouse intestinal flora quantity of table 5 (CFU/g)
Found out by table 5 is available, after experimental group 1 and experimental group 2 gavage, beneficial bacterium Bifidobacterium in mouse intestinal and Lactobacillus increased significantly, and harmful bacteria enterobacteria, enterococcus and C.perfringens significantly reduce, but from Microflora From the point of view of comparison, the effect of experimental group 2 will be substantially better than experimental group 1, this illustrates the common probiotics on this product and current market Product is compared, and the effect for adjusting intestinal flora is more preferable.And control group gavage before with gavage rear each Microflora without significant changes, This illustrates that the probiotics functional food of invention can effectively improve probiotics quantity in enteron aisle, inhibits harmful bacteria proliferation, Adjust intestinal flora balance.
Embodiment 3: clinical therapeutic efficacy
Clinical examination is used for after 30h being lyophilized in probiotics fermention food vaccum freezing dryer obtained in embodiment 1-4 It tests, 20 patients of each case, men and women is fifty-fifty, takes orally seven, twice a day, each 5g, therapeutic effect is shown in Table 6.
Therapeutic effect of the 6 this patent product of table to different enteric disorders
Illness It cures It is effective Effectively In vain
Diarrhea 20 0 0 0
Constipation 20 0 0 0
Colitis 18 2 0 0
From the point of view of clinical trial effect, the probiotics fermention food of invention has a variety of intestines problems excellent Therapeutic effect plays the effect of bidirectional modulation to diarrhea and constipation.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, It should be pointed out that anyone skilled in the art is in the technical scope disclosed by the present invention, skill according to the present invention Art scheme and its inventive concept are subject to equivalent substitution or change, should be covered by the protection scope of the present invention, and therefore, this is specially The scope of protection shall be subject to the appended claims for benefit.

Claims (4)

1. it is a kind of adjust intestinal health probiotics fermention functional food, which is characterized in that be by after soybean powder in proportion with Water mixes access probiotics and carries out segmentation solid state fermentation and obtain, and the segmentation solid state fermentation is specific as follows:
1. making withered in every gram of fermentation medium in the bacillus subtilis that activation is completed and bacillus coagulans access culture medium The inoculum concentration of careless bacillus is 105-106CFU/g, the inoculum concentration of bacillus coagulans are 106-107CFU/g maintains temperature 37 DEG C, ventilatory capacity 0.1-0.5m3/ min, ferment 10-18h；
2. 1. being walked after fermentation to the, activated animal bifidobacteria, Pediococcus acidilactici and lactobacillus plantarum are accessed, made The inoculum concentration of B. animais is 10 in every gram of fermentation medium6-107CFU/g, the inoculum concentration of Pediococcus acidilactici are 106- 107CFU/g, the inoculum concentration of lactobacillus plantarum are 105-106CFU/g maintains 37 DEG C of temperature, sealed fermenting 12-24h；
3. 2. being walked after fermentation to the, activated bifidobacterium adolescentis, long Bifidobacterium Bifidum and lactobacillus paracasei are accessed, Make the inoculum concentration 10 of bifidobacterium adolescentis in every gram of fermentation medium5-106CFU/g, the inoculum concentration of long Bifidobacterium Bifidum are 106- 107CFU/g, the inoculum concentration of lactobacillus paracasei are 106-108CFU/g maintains 37 DEG C of temperature, sealed fermenting 18-36h；
4. 3. being walked after fermentation to, activated lactobacillus reuteri is accessed, Luo Yishi in every gram of fermentation medium is made The inoculum concentration of lactobacillus is 107-108CFU/g maintains 37 DEG C of temperature, sealed fermenting 24-48h.
2. a kind of probiotics fermention functional food for adjusting intestinal health as described in claim 1, which is characterized in that soy meal Mass volume ratio with water is 1: 1.2-1: 2.0.
3. a kind of probiotics fermention functional food for adjusting intestinal health as described in claim 1, which is characterized in that fermentation knot Shu Hou is put into vacuum freeze drier and 24-35h is lyophilized, and the probiotics fermention functional food for adjusting intestinal health is made.
4. a kind of probiotics fermention functional food for adjusting intestinal health as described in claim 1, which is characterized in that
The lactobacillus plantarum is lactobacillus plantarum (Lactobacillus plantarum) IOB602, deposit number CGMCC No.16021；
The animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) IOB402, and deposit number is CGMCC No.16028；
The lactobacillus paracasei is lactobacillus paracasei (Lactobacillus paracasei) IOB413, and deposit number is CGMCC No.16022；
The bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacteriumadolescentis) IOB506, and preservation is compiled Number be CGMCC No.16026；
The bacillus coagulans are bacillus coagulans (Bacillus coagulans) IOB502, deposit number are as follows: CGMCC No.16025；
The bacillus subtilis is bacillus subtilis (Bacillus subtilis) IOB430, deposit number CGMCC No.16024；
The lactobacillus reuteri is lactobacillus reuteri (Lactobacillus reuteri) IOB423, and deposit number is CGMCC No.16023；
The bifidobacterium longum is bifidobacterium longum (Bifidobacterium longum) IOB515, deposit number CGMCC No.16027；
The Pediococcus acidilactici is Pediococcus acidilactici (Pediococcus acidilactici) IOB701, and deposit number is CGMCC No.16077。
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CN111955719A (en) * 2020-07-06 2020-11-20 齐鲁工业大学 Special medical food for treating inflammatory bowel disease and preparation method thereof
CN112980737A (en) * 2021-04-01 2021-06-18 江南大学 Bifidobacterium adolescentis for promoting proliferation of animal bifidobacterium and application thereof
CN113080461A (en) * 2021-05-14 2021-07-09 艾兰得生物科技研究泰州有限公司 Probiotics with function of inhibiting growth of proteus mirabilis as well as fermentation liquor and application thereof
CN114774307A (en) * 2022-03-30 2022-07-22 深圳龙宫伊莱特生物科技有限公司 Composite probiotic, fermented feed and preparation method thereof
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CN109805232A (en) * 2019-03-01 2019-05-28 天津科技大学 Jujube three-stage probiotics fermention produces Low acid micro-ecological foods

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CN106472704A (en) * 2016-09-27 2017-03-08 天津科技大学 Bean milk fermenting and producing probiotic bacteria functional drinkss and food and preparation method thereof
CN109805232A (en) * 2019-03-01 2019-05-28 天津科技大学 Jujube three-stage probiotics fermention produces Low acid micro-ecological foods

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CN111685310A (en) * 2020-06-04 2020-09-22 包选平 Composite edible fungus enzyme and preparation method thereof
CN111955719A (en) * 2020-07-06 2020-11-20 齐鲁工业大学 Special medical food for treating inflammatory bowel disease and preparation method thereof
CN112980737A (en) * 2021-04-01 2021-06-18 江南大学 Bifidobacterium adolescentis for promoting proliferation of animal bifidobacterium and application thereof
CN113080461A (en) * 2021-05-14 2021-07-09 艾兰得生物科技研究泰州有限公司 Probiotics with function of inhibiting growth of proteus mirabilis as well as fermentation liquor and application thereof
CN114774307A (en) * 2022-03-30 2022-07-22 深圳龙宫伊莱特生物科技有限公司 Composite probiotic, fermented feed and preparation method thereof
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CN115517367A (en) * 2022-11-28 2022-12-27 广东益可维生物技术有限公司 Application of lactobacillus paracasei SMN-LBK in preparation of product for promoting intestinal health
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Application publication date: 20191203