CN110506758A - Application of the artemisiifolia essential oil as herbicide - Google Patents
Application of the artemisiifolia essential oil as herbicide Download PDFInfo
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- CN110506758A CN110506758A CN201910959911.XA CN201910959911A CN110506758A CN 110506758 A CN110506758 A CN 110506758A CN 201910959911 A CN201910959911 A CN 201910959911A CN 110506758 A CN110506758 A CN 110506758A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
A kind of application the present invention relates to artemisiifolia essential oil as herbicide, after the artemisiifolia essential oil is dissolved with aqueous acetone solution, 20-50 times of dilution, it is sprayed before and after the emergence of weeds in field herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus according to 50 μ l/ml of spraying concentration, show through test result: when artemisiifolia essential oil is in 0.5 μ l/ml of low concentration, the growth of single broadleaf weed seedling can be significantly inhibited；When reaching 2 μ l/ml or more using concentration, subject plant herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus growth of seedling inhibited to influence by 50% or more；When concentration reaches 5 μ l/ml, germination is totally constrained or seedling is seriously poisoned and it is dead；Artemisiifolia essential oil is natural component, rapid decomposable in nature, free from environmental pollution, can be applied to the weed control of organic agriculture.Artemisiifolia essential oil is extracted by the aerial part of artemisiifolia plant, not only improves environmental protection, and comprehensively utilized to noxious plant.
Description
Invention field
The present invention relates to will extract from application of the aerial part essential oil of Alien Invasive Plants artemisiifolia plant as herbicide.
Background technique
The probability for successfully developing novel herbicide using traditional random synthesis filtering mode is smaller and smaller, and synthesizes chemistry
Environmental toxicity caused by herbicide is easy to retain for a long time in nature and drug injury problem are also got worse.And it is mentioned from nature
The synthesis herbicides such as the compounds with herbicidal activity from natural products taken has easily biological-degradable, noresidue, toxicity is low, development cost are few can not
The advantage of analogy, research are increasingly subject to the attention of people.Although certain chemical herbicides can decompose in the soil, by
In being applied in large quantities for a long time, weeds gradually produce resistance to herbicide, even lose so that drug effect reduces.In such case
Under, researching and developing, there is the herbicide of novel action target spot and the mechanism of action to be particularly important.
The natural products that type is abundant in nature, configuration is different, the mechanism of action is various provides possibility thus.Generation at present
In boundary it has been found that hundreds of tool activity of weeding native compound, some have been developed as herbicide promote and apply in addition some
The activity of weeding of natural products itself can match in excellence or beauty with chemical herbicide, such as 1,8- cineole.It is reported that artificial synthesized plant poison
The few overlappings of molecular target of element and the effect of natural plants toxin, may thus be sent out by the secondary compound of plant source
Now there is new target site, and environment amenable herbicide.
Artemisiifolia (Ambrosia artemisiifolia L.) is one kind of composite family Ambrosia, annual malignant weed.From
Start the 1930s after being overseas passed to Northeast China San Sheng, this worldwide harm grass is spread in the whole nation.It forms
To the significant damage of natural vegetation, agricultural production, referring to document 1-3.
Artemisiifolia essential oil, which is reported, tags isoreactivity with suppressing growth of microorganism and have to Field Pests, but artemisiifolia is smart
Oil has not been reported the growth inhibition effect of weeds in field, referring to document 4-8.External artemisiifolia is due to growing rapidly, viability
By force, (referring to document 9-10) is stablized in the agriculture forest and husbandry production and the ecosystem for seriously endangering invasion ground.Essence is extracted with its plant
Oil not only efficiently uses it, and controls its sprawling expansion.
Bibliography:
1, Wang Rui, Zhou Zhongshi, Zhang Guoliang, Guo Jianying, noble guest, Sun Yufang, Fu Weidong, Zhang Fudou, great incomparably are great outer
Carry out invasive weed and endangers pattern and Sustainable Treatment [J] bio-safety journal, 2018,27 (04), 317- in the distribution in China
320.
2, the main harm of Qu Zhi artemisiifolia and control method [J] are newly agriculture, and 2016, (14), 39.
3, the invasion speed of Dong Hegan, Song Zhanli Ili River Valley artemisiifolia and Ambrosia trifida, harm and Preventing Countermeasures [J]
Agriculture in Xinjiang science and technology, 2017, (05), 45-46.
4、Vidotto F,Tesio F,Ferrero A.Allelopathic effects of Ambrosia
artemisiifolia L.in the invasive process[J].Crop Protection,2013,54(12),161-
167.
5、Brückner,D.J.The allelopathic effect of ragweed(Ambrosia
artemisiifolia L.)on the germination of cultivated plants.[J].Novenytermeles,
1998,47(6),635-644.
6, the GC and GC/MS of Wang great Li, Zhu Xinru artemisiifolia and Ambrosia trifida volatile constituent analyze [J] mass-spectrometry
Report, 1996,17 (3), 37-41.
7, STUDY ON ALLELOPATHIC EFFECTS [J] Acta Ecologica Sinica of Wang great Li, Zhu Xinru artemisiifolia, 1996,16 (1), 11-19.
8, Chen Xianxing, the artemisiifolia that singly becomes reconciled are (natural to the Journal of Hainan University allelopathic effect [J] of several industrial crops
Scientific version), 2003,21 (1), 69-73.
9, the main harm of Liu Yuchun artemisiifolia and prevention and control measure [J] Agriculture in Hunan science, 2012, (24), 75-75.
10, Wang Meixiang, multitude's chess is auspicious, Sun Zhongxing, Wen Jingtian, the harm of Li Guohua artemisiifolia and section, the Guangdong agriculture prevention and control [J]
It learns, 2009, (10), 110-111.
Summary of the invention
Application the object of the present invention is to provide a kind of artemisiifolia essential oil as herbicide, by the artemisiifolia essential oil concentration
After the dissolution of 0.5% aqueous acetone solution, 20-50 times is diluted, is emerged in weeds in field herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus
Front and back is sprayed according to 50 μ l/ml of spraying concentration, when artemisiifolia essential oil is in 0.5 μ l/ml of low concentration, can be significantly inhibited single dicotyledonous
The growth of weeds seedling；When reaching 2 μ l/ml or more using concentration, subject plant herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus
Growth of seedling by 50% or more inhibited influence；When concentration reaches 5 μ l/ml, germination is totally constrained or seedling
It is seriously poisoned and dead；Artemisiifolia essential oil is natural component, rapid decomposable in nature, free from environmental pollution, be can be applied to
The weed control of organic agriculture；Artemisiifolia essential oil is extracted by the aerial part of artemisiifolia plant, not only improves environmental protection, and to noxious plant
It is comprehensively utilized.
The artemisiifolia essential oil concentration is 0.5% the third by a kind of application of the artemisiifolia essential oil of the present invention as herbicide
After ketone aqueous dissolution, 20-50 times is diluted, according to spray before and after the emergence of weeds in field herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus
50 μ l/ml of concentration sprinkling is applied, amount of application is 50-200 grams per acre.
A kind of application of the artemisiifolia essential oil of the present invention as herbicide, wherein artemisiifolia essential oil is water by conventional method
Steam distillation method is obtained from the aerial part of artemisiifolia plant, and derived essential oil is naturally occurring in artemisiifolia plant, can be in nature
It decomposes, therefore environment will not be polluted, acquisition time in August, 2019, collecting location: the Xinjiang Uygur Autonomous Regions state Yi Li
Xinyuan County.
Application of a kind of artemisiifolia essential oil of the present invention as herbicide, with weeds in field herba setariae viridis, annual bluegrass, clover
With for Amaranthus retroflexus be used as research object, find artemisiifolia essential oil at low concentration (0.5 μ l/ml) i.e. to herba setariae viridis, annual bluegrass, lucerne
The production of the growth of seedling of Mu and Amaranthus retroflexus significantly affects.This growth inhibition effect is gradually reinforced with increasing for concentration, when dense
When degree reaches 2 μ l/ml, in addition to the inhibiting rate to clover height of seedling is 26.2%, to the root long of herba setariae viridis, annual bluegrass and Amaranthus retroflexus
Reach 50% or more with the inhibiting rate of height of seedling.When concentration continues to be increased to 5 μ l/ml, inhibit the root long of clover and height of seedling equal
Between 80-90%, remaining weed seed is killed, and all loses germinating capacity；In potted plant experiment, artemisiifolia essential oil is in 50 μ
Above four kinds of weeds are sprayed under the concentration of l/ml, the performance seedling albefaction phenomena of mortality；The artemisiifolia essential oil removes weeds in field
Careless activity there is no report both at home and abroad at present.
A kind of application of the artemisiifolia essential oil of the present invention as herbicide, artemisiifolia essential oil main chemical compositions are shown in Table 1,
Shown artemisiifolia essential oil has 35 kinds of chemical components in table 1, and the 96.12% of the total derived essential oil of Zhan, it represents and plays activity of weeding
Main chemical compositions.
The main chemical compositions of 1 artemisiifolia essential oil of table
A kind of application of the artemisiifolia essential oil of the present invention as herbicide, artemisiifolia essential oil can be as Common Herbicides
It uses；It can be configured to various concentration, before emergence or seedling stage individually or with other pestsides synthesis uses, with concentration 0.5%
After aqueous acetone solution dissolution, preceding 20-50 times of dilution is being used, is being sprayed in weeds emergence front and back according to spraying concentration 20-50 μ l/ml
It spills.
A kind of application of the artemisiifolia essential oil of the present invention as herbicide, its advantages:
1. artemisiifolia essential oil provided by the invention, can be to four kinds of ruderal herba setariae viridis, morning in 0.5 μ l/ml of low concentration
The growth of seedling generation of ripe standing grain, clover and Amaranthus retroflexus significantly inhibits, and this growth inhibition effect is with increasing for concentration and gradually
Reinforce, when concentration reaches 1 μ l/ml, to the root long inhibiting rate of herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus reach 25% with
On；When concentration reaches 2 μ l/ml, 57.4% is followed successively by the inhibiting rate of the root long of herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus,
72.5%, 52.6% and 93.8%, the inhibiting rate to seedling is 64.2%, 76.8%, 26.2% and 93.1% respectively；Concentration liter
When up to 5 μ l/ml, herba setariae viridis, annual bluegrass and Amaranthus retroflexus seed are all killed, even if alfalfa seed can germinate, but after germination
Also browning is seriously poisoned, the seedling under the processing of this concentration then stops growing and gradually death.
2. artemisiifolia essential oil, which is suitably applied in farmland, carries out preventing and kill off for weeds, which is natural products, artemisiifolia essence
Oil is that steam distillation is obtained from the aerial part of artemisiifolia plant by conventional method, derived essential oil day in artemisiifolia plant
So exist, can be decomposed in nature, therefore environment will not be polluted.The weeds that can be applied in organic agriculture production are anti-
It removes.The essential oil shows very strong activity of weeding to a kind of exotic invasive injurious weed Amaranthus retroflexus, can be applied to carry out them
Prevention and control.In addition, the artemisiifolia extraction of essential oil is from exotic invasive noxious plant artemisiifolia, this instruction plant is in great numbers in field grown,
Viability is strong, and biomass is high, therefrom extracts artemisiifolia essential oil as herbicide and not only improves environmental protection, and has carried out to noxious plant comprehensive
It closes and utilizes, be to kill two birds with one stone.
Detailed description of the invention
Fig. 1 is inhibiting effect figure of the artemisiifolia essential oil of the present invention to herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus seedling root long,
Wherein, activity is respectively 0.5,1,2,5 μ l/ml；
Fig. 2 is inhibiting effect figure of the artemisiifolia essential oil of the present invention to herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus seedling height of seedling,
Wherein, activity is respectively 0.5,1,2,5 μ l/ml；
Fig. 3 is that artemisiifolia essential oil of the present invention is sprayed to outdoor potting herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus growth of seedling shadow
Ring action diagram, wherein spraying concentration is 50 μ l/ml.
Specific embodiment
In the following, illustrating the present invention for embodiment, still, the present invention is not limited to following embodiments.
What all raw and auxiliary materials, reagent and the instrument selected in the present invention were all well known in the art, other it is well known that
Some reagents and equipment be applied both to the implementation of following implementation of the present invention.
Embodiment 1
Influence of the artemisiifolia essential oil to weeds herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus amaranth growth of seedling:
The artemisiifolia above-ground plant parts of field acquisition is cut into segment to put into essential oil apparatus for extractive distillation, is distilled 3-6 hours,
Obtain artemisiifolia essential oil；
It is after 0.5% aqueous acetone solution dissolves by artemisiifolia essential oil concentration, being successively configured to concentration is 0.5 μ l/ml, 1 μ l/
Ml, 2 μ l/ml, 5 μ l/ml essential oil suspensions pipette 4ml various concentration after placing filter paper in the culture dish of diameter 9cm respectively
Essential oil suspension be added in different culture dishes, one plate of a concentration only places a kind of weed seed in one plate
50, it is each reprocessing three times, seal with sealing film be placed on cultivate 5 days in 25 DEG C of incubators of temperature after measurement seedling seedling
High and root long；
Data statistical approach: detect whether the difference between each group of data reaches significantly with single factor test variance first, Zhi Houyong
LSD method analyzes data, and the different group difference of Reference character reaches significantly, and level is p < 0.05；
As a result: under the low concentration effect of 0.5 μ l/ml, four kinds of weeds herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus seedling
Growth it is significantly affected, and with the raising of concentration, degree of susceptibility gradually enhances；When concentration reaches 0.5 μ l/
When ml, the root long of herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus is inhibited to be followed successively by 26.5%, 25.9%, 32.5% and 24%；Suppression
Herba setariae viridis processed, annual bluegrass, clover and Amaranthus retroflexus height of seedling be followed successively by 19.8%, 7.4%, 0% and 6.2%；Concentration is increased to 5 μ
When l/ml, herba setariae viridis, annual bluegrass and Amaranthus retroflexus seed are all killed, and the root long and height of seedling of clover are only 13% He compareed
11%, and seedling becomes brown and rots, and prompts seedling by murder by poisoning very serious, artemisiifolia essential oil is to subject plant seedling seedling
High influence is similar to the influence to root long, has the function of stronger inhibition growth, effect is referring to Fig. 1 and Fig. 2.
Embodiment 2
Artemisiifolia essential oil, which sprays, influences outdoor potting herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus growth of seedling:
Method: outdoor potting is carried out to weeds herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus, each flowerpot is interior, and there are 30
Plant, inhibiting effect of the detection artemisiifolia essential oil to outdoor weeds seedling；
By the artemisiifolia essential oil aqueous dissolution of 0.5% acetone of concentration, dilution is configured to 50 μ l/ml of concentration, sufficiently shakes up
The potted plant of the weeds herba setariae viridis of growth 4 weeks, annual bluegrass, clover and Amaranthus retroflexus is uniformly sprayed at after suspension, every basin is planted
Strain sprays the essential oil suspension of 10ml, and observation is to plant toxic effect after 12h overnight；
As a result: after touching artemisiifolia essential oil 12h, weeds herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus are shown significantly
Withered and albinism shows essential oil to the toxic action of weeds, this is to injure plant after artemisiifolia essential oil touches plant leaf blade
Cell makes cell dehydration lose viability, to inhibit weed growth, specific effect is referring to Fig. 3.
Conclusion:
A kind of application of the artemisiifolia essential oil of the present invention as herbicide, by experiments have shown that: in 0.5 μ l/ of low concentration
When ml, the growth of seedling of four kinds of tested weeds herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus can be significantly inhibited；When use concentration
When reaching 2 μ l/ml or more, herba setariae viridis, annual bluegrass, clover and 80% growth of seedling of Amaranthus retroflexus are suppressed influence；When concentration reaches
When to 5 μ l/ml, herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus germination are totally constrained or seedling is seriously poisoned and it is dead
It dies；Outdoor potted plant experiment also further demonstrates the potentiality that artemisiifolia essential oil has exploitation herbicide, and artemisiifolia essential oil is by artemisiifolia plant
Aerial part extract, not only improve environmental protection, and comprehensively utilized to noxious plant；Artemisiifolia essential oil is natural component, In
Nature is rapid decomposable, free from environmental pollution, can be applied to the weed control of organic agriculture；The present invention is smart by the artemisiifolia provided
Oily herbicide can protrude it and be derived from plant, can decompose in nature, and environmental protection is free from environmental pollution, while plant to nocuousness
Object has carried out waste utilization.
Claims (1)
1. a kind of application of artemisiifolia essential oil as herbicide, which is characterized in that by the artemisiifolia essential oil concentration be 0.5% acetone water
After solution dissolution, 20-50 times is diluted, it is dense according to spraying before and after the emergence of weeds in field herba setariae viridis, annual bluegrass, clover and Amaranthus retroflexus
50 μ l/ml sprinkling is spent, amount of application is 50-200 grams per acre.
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| CN201910959911.XA CN110506758A (en) | 2019-10-10 | 2019-10-10 | Application of the artemisiifolia essential oil as herbicide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111264120A (en) * | 2020-02-19 | 2020-06-12 | 楚雄宏丰农业科技开发有限公司 | Method for inhibiting weed seed germination by using natural capsaicin aqueous emulsion |
| CN111758726A (en) * | 2020-07-07 | 2020-10-13 | 中国科学院新疆生态与地理研究所 | Application of beta-eucalyptol in preparation of herbicide |
| CN115486465A (en) * | 2022-10-25 | 2022-12-20 | 长春师范大学 | Bidens pilosa herbicide based on Ambrosia trifoliata |
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| CN107736384A (en) * | 2017-10-30 | 2018-02-27 | 韦玉国 | A kind of plant herbicide |
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2019
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107736384A (en) * | 2017-10-30 | 2018-02-27 | 韦玉国 | A kind of plant herbicide |
Non-Patent Citations (3)
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| JUDITH M. BRADOW: "《JUDITH M. BRADOW》", 17 December 1985 * |
| 王朋 等: "植物挥发性单萜经土壤载体的化感作用——以三裂叶豚草（Ambrosia trifida L.）为例", 《生态学报》 * |
| 黄蕊 等: "豚草（Ambrosia artemisiifolia L.）中化学成分及其生物活性的研究进展", 《华中昆虫研究（第九卷）》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111264120A (en) * | 2020-02-19 | 2020-06-12 | 楚雄宏丰农业科技开发有限公司 | Method for inhibiting weed seed germination by using natural capsaicin aqueous emulsion |
| CN111758726A (en) * | 2020-07-07 | 2020-10-13 | 中国科学院新疆生态与地理研究所 | Application of beta-eucalyptol in preparation of herbicide |
| CN115486465A (en) * | 2022-10-25 | 2022-12-20 | 长春师范大学 | Bidens pilosa herbicide based on Ambrosia trifoliata |
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