CN110501321B - Portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection - Google Patents
Portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection Download PDFInfo
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- CN110501321B CN110501321B CN201910701727.5A CN201910701727A CN110501321B CN 110501321 B CN110501321 B CN 110501321B CN 201910701727 A CN201910701727 A CN 201910701727A CN 110501321 B CN110501321 B CN 110501321B
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Abstract
The invention discloses a portable optical path system integrating Raman spectrum fast imaging and deep spectrum fast detection, which comprises: first beam collimator, the telescope tube, the band pass filter, convex lens, conical lens, the dichroscope, the second beam collimator, imaging lens, the fiber bundle, achromatic double-cemented lens pair and one-dimensional scanning galvanometer, wherein, convex lens and conical lens are all installed in telescope tube with pluggable form, the reflective surface of dichroscope is towards the telescope tube, second beam collimator and imaging lens are installed respectively in the reflected light and the transmission light exit slot department of dichroscope, the fiber bundle is installed at imaging lens's the other end, the optic fibre of collection end and sense terminal is square and rectangle array respectively and arranges, achromatic double-cemented lens pair is installed at the spectral detection end of fiber bundle, one-dimensional scanning galvanometer installs the other end at lens pair. The invention has the advantages that: the volume is small and exquisite, conveniently carries the removal to formation of image and detection speed are very fast.
Description
Technical Field
The invention relates to an optical path system, in particular to a portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection, and belongs to the technical field of photoelectric detection.
Background
With the development of laser technology, optical fiber detection devices and photoelectric detection technology, Raman Spectral Imaging (RSI) technology has developed into a relatively mature technology capable of qualitatively, quantitatively and positionally describing the information such as biochemical composition type, content and distribution of a detected sample. The RSI technology integrates the advantages of the spectrum technology and the imaging technology, can effectively break through the objective influence of the complexity of the biological tissue structure on the spectrum analysis result, and reveals the biochemical composition and the tissue structure characteristics of the sample in a mode of combining a spectrum (characteristic spectrum) diagram (spectrum image) with high content, high specificity and high accuracy. Therefore, the RSI technology for observing information such as substance components and structures in an image form has important application value in the biomedical fields such as clinical cancer early diagnosis, histopathological physiological analysis, disease mechanism and the like.
However, in the field of biomedical research, the existing RSI technology is not perfect enough, and some problems still exist, such as:
(1) point scanning imaging is generally adopted, so the imaging speed is slow;
(2) because of adopting the microscopic light path, the view field is narrower;
(3) because Coherent Anti-stokes Raman Spectroscopy (CRS) and Stimulated Raman Scattering (SRS) need to adopt a pulse laser light source, the system structure is more complex and the portability is poorer;
(4) because the photon scattering transport length in the medium is short, only near-surface spectrum information within hundreds of micrometers of a detected sample can be detected.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a portable optical path system integrating rapid Raman spectrum imaging and rapid deep spectrum detection.
In order to achieve the above object, the present invention adopts the following technical solutions:
the utility model provides a collect quick formation of image of raman spectrum and deep spectrum short-term test in portable optical path system of an organic whole which characterized in that includes: first beam collimator, telescope tube, band pass filter, convex lens, cone lens, dichroscope, second beam collimator, imaging lens, fiber bundle, achromatic double-cemented lens pair and one-dimensional scanning mirror that shakes, wherein:
the telescopic sleeve consists of an inner cylinder and an outer cylinder, and the inner cylinder can axially slide back and forth in the outer cylinder;
the first beam collimator is arranged at the outer end of the inner cylinder of the telescopic sleeve;
the band-pass filter, the convex lens and the cone lens are all arranged in an inner cylinder of the telescopic sleeve, wherein the tip of the cone lens deviates from the first beam collimator, and the convex lens and the cone lens can be inserted and pulled in the telescopic sleeve at will;
the dichroic mirror is arranged in a dichroic mirror fixing box, the dichroic mirror fixing box is arranged at the outer end of the outer barrel of the telescopic sleeve, the dichroic mirror and the axis of the telescopic sleeve form an included angle of 45 degrees, and the light reflecting surface faces the telescopic sleeve;
the second beam collimator is arranged at the reflected light outlet of the dichroic mirror fixing box;
the imaging lens is arranged in the lens fixing barrel, and the lens fixing barrel is arranged at a transmission light outlet of the dichroic mirror fixing box;
the optical fiber bundle is arranged at the other end of the imaging lens, the optical fibers at the spectrum acquisition end of the optical fiber bundle are arranged in a square array, and the optical fibers at the spectrum detection end of the optical fiber bundle are arranged in a rectangular array;
the achromatic double-cemented lens pair is arranged in the lens pair fixing barrel, and the lens pair fixing barrel is arranged at the spectrum detection end of the optical fiber bundle;
the one-dimensional scanning galvanometer is arranged in the galvanometer fixing box, the galvanometer fixing box is arranged at the other end of the lens pair fixing cylinder, and the one-dimensional scanning galvanometer forms an included angle of 45 degrees with the axis of the achromatic double-cemented lens pair.
Aforementioned collection raman spectrum fast imaging and deep layer spectrum short-term test in portable optical path system of an organic whole, its characterized in that is provided with filter mount pad and lens mount pad in aforementioned telescope tube's the inner tube to the inner tube is opened in lens mount pad position department and is used for getting of putting convex lens and awl lens and puts the mouth.
The portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection is characterized in that the inner cylinder of the telescopic sleeve is cylindrical, the outer diameter of the inner cylinder is 25mm, the length of the inner cylinder is 50mm, the outer cylinder of the telescopic sleeve is cylindrical, the outer diameter of the outer cylinder is 30mm, and the length of the outer cylinder is 50 mm.
The portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection is characterized in that the dichroic mirror fixing box is in a cube shape, and the side length is 30 mm.
The portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection is characterized in that the lens fixing cylinder is cylindrical, the outer diameter of the lens fixing cylinder is 25mm, and the length of the lens fixing cylinder is 40 mm.
The portable optical path system integrating rapid imaging of Raman spectrum and rapid detection of deep spectrum is characterized in that the arrangement size of the optical fibers at the spectrum detection end of the optical fiber bundle depends on the width and height of the slit of the imaging spectrometer, and the size and deflection angle of the one-dimensional scanning galvanometer need to be effectively matched.
The portable optical path system integrating the rapid Raman spectrum imaging and the rapid deep spectrum detection is characterized in that the shortest length of the optical fiber bundle is 300 mm.
The portable optical path system integrating rapid Raman spectrum imaging and rapid deep spectrum detection is characterized in that a notch filter or a long-pass filter group is integrated between two lenses of the achromatic double-cemented lens pair.
The portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection is characterized in that the lens is in a long cylinder shape relative to the fixed cylinder, the outer diameter of the lens is 25mm, and the length of the lens is 100 mm.
The portable optical path system integrating Raman spectrum rapid imaging and deep spectrum rapid detection is characterized in that the galvanometer fixing box is cube-shaped, and the side length is 40 mm.
The invention has the advantages that:
(1) on the basis of a Raman spectral imaging light path, a reverse space Offset Raman Spectroscopy (Inverse SORS) detection method is integrated, so that organic combination of shallow surface layer Raman spectral imaging and deep layer spectral detection is realized;
(2) raman spectrum information at different depth positions of the sample is detected by optimizing a spectrum excitation and detection mode and controlling continuous change of space offset (size of an annular excitation light spot), deep biochemical composition information of the tissue is comprehensively and accurately reflected, and the method can be used for detecting and analyzing information of multilayer materials such as chemistry, biology, physics and the like;
(3) the device has small volume, is convenient to carry and move, has higher imaging and detection speed, and is suitable for clinical detection of deep substance components and structural information of normal biological tissues and canceration biological tissues under living conditions.
Drawings
FIG. 1 is an optical path diagram of a portable optical path system provided by the present invention in Raman spectrum imaging;
FIG. 2 is a light path diagram of the portable light path system provided by the present invention during deep spectrum detection;
FIG. 3(a) is a schematic view of the optical fiber arrangement at the spectrum collection end of the optical fiber bundle;
fig. 3(b) is a schematic view of the optical fiber arrangement at the spectrum detection end of the optical fiber bundle.
The meaning of the reference symbols in the figures: the device comprises a 1-785nm laser, a 2-first beam collimator, a 3-telescopic sleeve, a 4-band-pass filter, a 5-convex lens, a 6-conical lens, a 7-dichroic mirror, an 8-second beam collimator, a 9-imaging lens, a 10-optical fiber bundle, an 11-achromatic double cemented lens pair, a 12-one-dimensional scanning galvanometer, a 13-imaging spectrometer and a 14-back photosensitive depth refrigeration electron multiplication type CCD camera.
Detailed Description
The invention is described in detail below with reference to the figures and the embodiments.
Referring to fig. 1 and 2, the portable optical path system integrating fast raman spectroscopy imaging and fast deep spectrum detection provided by the present invention includes: the device comprises a first beam collimator 2, a telescopic sleeve 3, a band-pass filter 4, a convex lens 5, a cone lens 6, a dichroic mirror 7, a second beam collimator 8, an imaging lens 9, an optical fiber bundle 10, an achromatic double-cemented lens pair 11 and a one-dimensional scanning galvanometer 12.
The telescopic sleeve 3 consists of an inner cylinder and an outer cylinder, the inner cylinder can axially slide back and forth in the outer cylinder, a filter mounting seat and a lens mounting seat are arranged in the inner cylinder, and a taking and placing opening for taking and placing the convex lens and the conical lens is formed in the position of the lens mounting seat of the inner cylinder. The inner cylinder and the outer cylinder are both cylindrical, wherein the inner cylinder can be made into a cylinder with the outer diameter of 25mm and the length of 50mm, the outer cylinder can be made into a cylinder with the outer diameter of 30mm and the length of 50mm, when the inner cylinder is completely retracted into the outer cylinder, the length of the whole telescopic sleeve 3 is only 50mm, and when the inner cylinder is pulled out of the outer cylinder to the maximum extent, the length of the whole telescopic sleeve 3 cannot exceed 100 mm.
The first beam collimator 2 is arranged at the outer end of the inner cylinder of the telescopic sleeve 3, and when the laser tube is used, the first beam collimator 2 is connected with the 785nm laser 1 through a coupling optical fiber.
Band-pass filter 4, convex lens 5 and axicon 6 are all installed in telescopic sleeve 3's inner tube, wherein, band-pass filter 4 is installed on the filter mount pad, convex lens 5 and axicon 6 are installed on the lens mount pad, the pointed end of axicon 6 deviates from first beam collimator 2, convex lens 5 and axicon 6 can plug at will on the lens mount pad, it is when convex lens 5 when installing on the lens mount pad, raman spectrum imaging can be realized to this light path system, when installing on the lens mount pad is axicon 6, deep spectral detection can be realized to this light path system.
The second beam collimator 8 is mounted at the reflected light exit opening of the dichroic mirror fixing box. The reflected light passes through the second beam collimator 8 to form a uniform collimated light beam which is irradiated on the surface of the sample.
The imaging lens 9 is arranged in the lens fixing barrel, and the lens fixing barrel is arranged at the transmission light outlet of the dichroic mirror fixing box. The lens fixing cylinder is cylindrical and can be made into a cylindrical long cylinder with the outer diameter of 25mm and the length of 40 mm.
The optical fiber bundle 10 is installed at the other end of the imaging lens 9, and the optical fibers at the spectrum collection end of the optical fiber bundle 10 are arranged in a square array (as shown in fig. 3(a), the area is 4 × 4mm2400 × 400 optical fibers with a core diameter of 10 μm and an NA of 0.6) can be used to completely collect tissue surface spectral information, and the optical fibers at the spectral detection end are arranged in a rectangular array (as shown in fig. 3(b), the area of the optical fibers is 2 × 8mm2200 × 800 optical fibers, 10 μm core diameter, and NA of 0.6), the size of the rectangular array depends on the width and height of the slit of the imaging spectrometer 13, and it is necessary to effectively match the size and deflection angle of the one-dimensional scanning galvanometer 12. The arrangement of the optical fibers can improve spectral image dataAcquisition efficiency and system signal-to-noise ratio. The minimum length of the optical fiber bundle 10 may be 300 mm.
The achromatic double-cemented lens pair 11 is arranged in a lens pair fixing barrel, the lens pair fixing barrel is arranged at the spectrum detection end of the optical fiber bundle 10, and a notch filter or a long-pass filter group is integrated between the two lenses. The lens is cylindrical relative to the fixed cylinder, and can be made into a cylindrical long cylinder with the outer diameter of 25mm and the length of 100 mm.
The one-dimensional scanning galvanometer 12 is arranged in a galvanometer fixing box, the galvanometer fixing box is arranged at the other end of the lens pair fixing cylinder, and the one-dimensional scanning galvanometer 12 forms a 45-degree included angle with the axis of the achromatic double-cemented lens pair 11. The galvanometer fixing box is in a cube shape and can be made into a square box with the side length of 40 mm.
The advantages of line-scan spectral imaging are: confocal-like forms can be used, the spectral imaging speed can be increased to a large extent with little cost, and the possibility of thermal decomposition or photochemical reactions of the sample can be reduced because the laser power is distributed in a line.
A line scanning mode is adopted at a spectrum detection end, so that the rapid screening and the recording of a spectrum data set with higher spectral resolution and a wider spectral band range are realized.
When the lens mounting base is provided with the convex lens 5, referring to fig. 1, the optical path system can realize raman spectral imaging, and the process of the spectral imaging is as follows:
a785 nm laser 1 is used as an excitation light source, an excitation light beam (a solid line) sequentially passes through a coupling optical fiber, a first beam collimator 2, a band-pass filter 4, a convex lens 5, a dichroic mirror 7 and a second beam collimator 8 to form a uniform collimated circular excitation light spot to irradiate on the surface of a sample, the size adjustment of the circular excitation light spot irradiated on the surface of the sample can be realized by adjusting the focal length and the spatial position of the convex lens 5, the optimal working distance of the light path system is 50-100mm, the optimal size (diameter) of the circular excitation light spot irradiated on the surface of the sample is 2-3mm, anisotropic scattered light (a dotted line) at different positions on the surface of the sample sequentially passes through the second beam collimator 8, the dichroic mirror 7 and an imaging lens 9 to be imaged on the spectrum acquisition end of an optical fiber bundle 10 completely, and the formed image is transmitted to an achromatic double cemented lens pair 11 by the optical fiber bundle 10, a notch filter (or a long-pass filter group) is integrated between the two lenses of the achromatic double-cemented lens pair 11, the one-dimensional scanning galvanometer 12 projects Raman scattering light at different spatial positions to the imaging spectrometer 13 after the Rayleigh scattering is filtered by the notch filter (or the long-pass filter group), and the imaging spectrometer 13 records and analyzes spectral information.
When the axicon lens 6 is mounted on the lens mounting seat, referring to fig. 2, the optical path system can implement deep spectrum detection based on an Inverse spatial Offset Raman Spectroscopy (Inverse SORS) detection method, and the spectrum detection process is as follows:
using a 785nm laser 1 as an excitation light source, forming a uniform collimated annular excitation light spot to irradiate on the surface of a sample after an excitation light beam sequentially passes through a coupling optical fiber, a first beam collimator 2, a band-pass filter 4, a cone lens 6, a dichroic mirror 7 and a second beam collimator 8, changing the cone lens 6 with different degrees (the selection range of the spatial offset between the excitation annular light spot and a point-shaped spectrum acquisition region is different according to the difference of the degrees of the cone lens 6. for example, when the degree of the cone lens 6 is 5 degrees, the maximum offset distance of the optical path system is 10mm), adjusting the position of the cone lens 6 (realized by drawing the inner cylinder of the telescopic sleeve 3) and adjusting the distance (working distance) from the second beam collimator 8 to the working surface, the size of the annular excitation light spot irradiated on the surface can be adjusted, and the radius (spatial offset Δ S) of the annular light spot can be controlled, the optimal working distance of the optical path system is 10-100mm, and the optimal size (diameter) of the annular excitation light spot irradiated on the surface of the sample is 4-6 mm. According to the photon migration theory, the point-like spectrum acquisition region can collect optical information in the spectrum information acquisition region (with the depth of about 0.5-2mm), and the optical information is transmitted to the imaging spectrometer 13 by the optical fiber bundle 10 for analysis, so that the Raman spectrum information at different depth positions of the sample is detected by controlling the continuous change of the spatial offset, namely the deep-layer spectrum detection function.
By combining a tissue optical model and the existing in-vitro section Raman spectrum experimental result, a theoretical simulation method is established and perfected, and the relation between the space offset Delta S and Inverse SORS detection is described theoretically.
It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the protection scope of the present invention.
Claims (1)
1. The utility model provides a collect quick formation of image of raman spectrum and deep spectrum short-term test in portable optical path system of an organic whole which characterized in that includes: first beam collimator (2), telescope tube (3), band-pass filter (4), convex lens (5), cone lens (6), dichroic mirror (7), second beam collimator (8), imaging lens (9), optical fiber bundle (10), achromatic double cemented lens pair (11) and one-dimensional scanning galvanometer (12), wherein:
the telescopic sleeve (3) consists of an inner cylinder and an outer cylinder, and the inner cylinder can axially slide back and forth in the outer cylinder;
the first beam collimator (2) is arranged at the outer end of the inner cylinder of the telescopic sleeve (3);
the band-pass filter (4), the convex lens (5) and the cone lens (6) are all installed in an inner cylinder of the telescopic sleeve (3), wherein the tip of the cone lens (6) deviates from the first beam collimator (2), and the convex lens (5) and the cone lens (6) can be inserted and pulled in the telescopic sleeve (3) at will;
the dichroic mirror (7) is arranged in a dichroic mirror fixing box, the dichroic mirror fixing box is arranged at the outer end of the outer cylinder of the telescopic sleeve (3), the dichroic mirror (7) and the axis of the telescopic sleeve (3) form an included angle of 45 degrees, and the reflecting surface faces the telescopic sleeve (3);
the second beam collimator (8) is arranged at the reflected light outlet of the dichroic mirror fixing box;
the imaging lens (9) is arranged in the lens fixing barrel, and the lens fixing barrel is arranged at a transmission light outlet of the dichroic mirror fixing box;
the optical fiber bundle (10) is arranged at the other end of the imaging lens (9), the optical fibers at the spectrum acquisition end of the optical fiber bundle (10) are arranged in a square array, and the optical fibers at the spectrum detection end are arranged in a rectangular array;
the achromatic double cemented lens pair (11) is arranged in a lens pair fixing barrel, and the lens pair fixing barrel is arranged at the spectrum detection end of the optical fiber bundle (10);
the one-dimensional scanning galvanometer (12) is arranged in a galvanometer fixing box, the galvanometer fixing box is arranged at the other end of the lens pair fixing cylinder, and the axis of the one-dimensional scanning galvanometer (12) and the axis of the achromatic doublet cemented lens pair (11) form an included angle of 45 degrees;
a filter mounting seat and a lens mounting seat are arranged in the inner cylinder of the telescopic sleeve (3), and a taking and placing opening for taking and placing the convex lens and the conical lens is formed in the position of the lens mounting seat of the inner cylinder;
the inner cylinder of the telescopic sleeve (3) is cylindrical, the outer diameter is 25mm, the length is 50mm, the outer cylinder is also cylindrical, and the outer diameter is 30mm, and the length is 50 mm;
the dichroic mirror fixing box is in a cube shape, and the side length is 30 mm;
the lens fixing cylinder is cylindrical, the outer diameter of the lens fixing cylinder is 25mm, and the length of the lens fixing cylinder is 40 mm;
the arrangement size of the optical fibers at the spectrum detection end of the optical fiber bundle (10) depends on the width and the height of a slit of an imaging spectrometer (13), and in addition, the size and the deflection angle of a one-dimensional scanning galvanometer (12) need to be effectively matched;
the shortest length of the optical fiber bundle (10) is 300 mm;
a notch filter or a long-pass filter group is integrated between two lenses of the achromatic double cemented lens pair (11);
the lens pair fixing cylinder is in a long cylinder shape, the outer diameter of the lens pair fixing cylinder is 25mm, and the length of the lens pair fixing cylinder is 100 mm;
the galvanometer fixing box is cubic, and the side length is 40 mm.
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| CN111044500B (en) * | 2019-12-30 | 2022-12-06 | 北京华泰诺安探测技术有限公司 | Raman probe capable of adjusting annular detection area |
| WO2021221569A1 (en) * | 2020-04-30 | 2021-11-04 | Agency For Science, Technology And Research | Rapid diagnostics for analyte/biomarker detection by raman technology with non-spectrometer raman measurement system |
| CN112326585B (en) * | 2020-10-28 | 2021-10-22 | 南京农业大学 | Infrared spectrum device and detection method for rapidly detecting powdery mildew of strawberries |
| CN115120233B (en) * | 2021-03-29 | 2024-04-16 | 上海近观科技有限责任公司 | Non-invasive biological characteristic signal detection device, system and method |
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| CN108693167A (en) * | 2018-07-24 | 2018-10-23 | 西北大学 | A kind of reverse spatial deviation Raman spectrum detecting device and method |
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