CN110501321A - Collection Raman spectrum fast imaging and deep layer spectrum are quickly detected on integrated portable light path system - Google Patents
Collection Raman spectrum fast imaging and deep layer spectrum are quickly detected on integrated portable light path system Download PDFInfo
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- CN110501321A CN110501321A CN201910701727.5A CN201910701727A CN110501321A CN 110501321 A CN110501321 A CN 110501321A CN 201910701727 A CN201910701727 A CN 201910701727A CN 110501321 A CN110501321 A CN 110501321A
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- extension sleeve
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
Abstract
The invention discloses a kind of collection Raman spectrum fast imagings and deep layer spectrum to be quickly detected on integrated portable light path system, comprising: the first beam collimator, extension sleeve, band pass filter, convex lens, axicon lens, dichroscope, second beam collimator, imaging len, fiber optic bundle, achromatic doublet to and one-dimensional scanning galvanometer, wherein, convex lens and axicon lens are mounted in extension sleeve in the form of pluggable, the reflective surface of dichroscope is towards extension sleeve, second beam collimator and imaging len are separately mounted at the reflected light and transmitted light light-emitting window of dichroscope, fiber optic bundle is mounted on the other end of imaging len, collection terminal and the optical fiber of test side are in the arrangement of square and rectangular array respectively, achromatic doublet is to the spectral detection end for being mounted on fiber optic bundle, one-dimensional scanning galvanometer is mounted on lens Pair the other end.The invention has the beneficial effects that: compact is convenient for carrying movement, and is imaged and detects fast speed.
Description
Technical field
The present invention relates to a kind of light path systems, and in particular to a kind of collection Raman spectrum fast imaging and deep layer spectrum are quickly examined
It surveys in the portable light path system of one, belongs to photoelectric detection technology field.
Background technique
With laser technology, the development of fiber-optic probe device and photoelectric detecting technology, Raman spectrum imaging (Raman
Spectral Imaging, RSI) technology has evolved into one kind can be by tested sample biochemical composition type, content and distribution
Etc. information carry out the technology of qualitative, quantitative and positioning describing comparative maturity.Two kinds of technologies of RSI technological incorporation spectrum and imaging
The advantages of, the objective influence that mechanics of biological tissue complexity generates Spectroscopic analysis results can be effectively broken through, it is (special with a kind of spectrum
Levy spectrum) figure (spectrum picture) combine mode, high intension, high specific, high accuracy disclose sample biochemical composition and tissue
Design feature.Therefore, with the RSI technology of the information such as image format observation material composition, structure in clinical cancer early diagnosis, group
It knits pathologic, physiologic analysis and there is important application value at fields of biomedicine such as the interpretation of the cause, onset and process of an illness.
However, existing RSI technology is perfect not enough, and there are still some problems in field of biomedical research, such as:
(1) Mapping is generallyd use, so image taking speed is slower；
(2) because using micro- optical path, so visual field is than narrow；
(3) because coherent antistockes Raman spectroscopy (Coherent Anti-strokes Raman Spectroscopy,
CRS pulsed laser light source) need to be used with stimulated Raman scattering (Stimulated Raman Spectroscopy, SRS), therefore is
Structure of uniting is complex, and portability is poor；
(4) because media interior photon equilibrium state transports, length is shorter, so several hundred microns of tested sample can only be detected
Interior nearly surface layer spectral information.
Summary of the invention
To solve the deficiencies in the prior art, the purpose of the present invention is to provide a kind of collection Raman spectrum fast imaging and deep layers
Spectrum is quickly detected on integrated portable light path system.
In order to achieve the above objectives, the present invention adopts the following technical scheme that:
A kind of collection Raman spectrum fast imaging and deep layer spectrum are quickly detected on integrated portable light path system, feature
It is, comprising: the first beam collimator, extension sleeve, band pass filter, convex lens, axicon lens, dichroscope, the second light beam
Collimator, imaging len, fiber optic bundle, achromatic doublet to and one-dimensional scanning galvanometer, in which:
Extension sleeve is made of inner barrel and outer barrel two parts, and inner cylinder can slidably reciprocate in outer cylinder along axial direction；
First beam collimator is mounted on the outer end of the inner cylinder of extension sleeve；
Band pass filter, convex lens and axicon lens are installed in the inner cylinder of extension sleeve, wherein are carried on the back at the tip of axicon lens
From the first beam collimator, convex lens and axicon lens can be plugged arbitrarily in extension sleeve；
Dichroscope is mounted in dichroscope fixed bin, and dichroscope fixed bin is mounted on the outer of the outer cylinder of extension sleeve
End, the axis of dichroscope and extension sleeve are in 45 degree of angles, and reflective surface is towards extension sleeve；
Second beam collimator is mounted at the reflected light light-emitting window of dichroscope fixed bin；
Imaging len is mounted in lens fixed cylinder, and the transmitted light that lens fixed cylinder is mounted on dichroscope fixed bin goes out light
At mouthful；
Fiber optic bundle is mounted on the other end of imaging len, the square array row of the optical fiber at the spectra collection end of fiber optic bundle
The optical fiber of cloth, spectral detection end is rectangle array arrangement；
For achromatic doublet to lens are mounted on in fixed cylinder, lens are mounted on the spectrum of fiber optic bundle to fixed cylinder
Test side；
One-dimensional scanning galvanometer is mounted in galvanometer fixed bin, and galvanometer fixed bin is mounted on lens to the other end of fixed cylinder,
The axis of one-dimensional scanning galvanometer and achromatic doublet pair is in 45 degree of angles.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is, filter plate mounting base and lens mounting base is provided in the inner cylinder of aforementioned extension sleeve, and inner cylinder is installed in lens
Seat position is provided with the pick-and-place mouth for picking and placing convex lens and axicon lens.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that the inner cylinder of aforementioned extension sleeve is cylindrical, and outer diameter 25mm, length 50mm, outer cylinder is also cylindrical, and outer diameter is
30mm, length 50mm.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that aforementioned dichroscope fixed bin is in cube shaped, side length 30mm.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that aforesaid lens fixed cylinder is cylindrical, outer diameter 25mm, length 40mm.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that the arrangement size of the optical fiber at the spectral detection end of aforementioned fiber optic bundle depends on the width and height of the slit of imaging spectrometer
Degree, further needs exist for the size and deflection angle that are effectively matched one-dimensional scanning galvanometer.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that it is 300mm that the length of aforementioned fiber optic bundle is most short.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is, notch filter piece or long pass filter piece group are integrated between two lens of aforementioned achromatic doublet pair.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that aforesaid lens are in long tubular, outer diameter 25mm, length 100mm to fixed cylinder.
Collection Raman spectrum fast imaging and deep layer spectrum above-mentioned are quickly detected on integrated portable light path system, special
Sign is that aforementioned galvanometer fixed bin is in cube shaped, side length 40mm.
The invention has the beneficial effects that:
(1) on the basis of Raman spectrum imaging optical path, reverse spatial offset Raman spectrum (Inverse has been incorporated
Spatially Offset Raman Spectroscopy, Inverse SORS) detection method, realize superficial layer Raman light
The combination of spectrum imaging and deep layer spectral detection；
(2) by the optimization excitation of spectra and detection mode, pass through control spatial offset (ring-type excitation spot size)
Consecutive variations, detect the Raman spectral information at sample different depth position, and comprehensive and accurate reflection tissue deep layer biochemistry is constituted
Information can be used for the multilayer materials infomation detection such as chemistry, biology, physics and analysis；
(3) compact is convenient for carrying movement, and is imaged and detects fast speed, is suitable for normal under condition of living body
Biological tissue and the deep layer material composition of canceration biological tissue and the clinical detection of structural information.
Detailed description of the invention
Fig. 1 is index path of the portable light path system provided by the invention in Raman spectrum imaging；
Fig. 2 is index path of the portable light path system provided by the invention in deep layer spectral detection；
Fig. 3 (a) is the optical fiber arrangement schematic diagram at the spectra collection end of fiber optic bundle；
Fig. 3 (b) is the optical fiber arrangement schematic diagram at the spectral detection end of fiber optic bundle.
The meaning of appended drawing reference in figure: 1-785nm laser, the first beam collimator of 2-, 3- extension sleeve, the filter of 4- band logical
Wave plate, 5- convex lens, 6- axicon lens, 7- dichroscope, the second beam collimator of 8-, 9- imaging len, 10- fiber optic bundle, 11- disappear
Color difference cemented doublet to, 12- one-dimensional scanning galvanometer, 13- imaging spectrometer, 14- carry on the back photosensitive deep refrigerating electron multiplication type CCD
Camera.
Specific embodiment
Specific introduce is made to the present invention below in conjunction with the drawings and specific embodiments.
Referring to Figures 1 and 2, collection Raman spectrum fast imaging and deep layer spectrum provided by the invention are quickly detected on integrated
Portable light path system include: the first beam collimator 2, extension sleeve 3, band pass filter 4, convex lens 5, axicon lens 6, two to
Look mirror 7, the second beam collimator 8, imaging len 9, fiber optic bundle 10, achromatic doublet are to 11 and one-dimensional scanning galvanometer
12。
Extension sleeve 3 is made of inner barrel and outer barrel two parts, and inner cylinder can slidably reciprocate in outer cylinder along axial direction, is set in inner cylinder
It is equipped with filter plate mounting base and lens mounting base, and inner cylinder is provided in lens mounting base position for picking and placing convex lens
With the pick-and-place mouth of axicon lens.Inner barrel and outer barrel is cylindrical, wherein inner cylinder can be made into that outer diameter is 25mm, length is 50mm's
Cylinder, outer cylinder can be made into the cylinder that outer diameter is 30mm, length is 50mm, entire flexible when inner cylinder is fully retracted in outer cylinder
The length of sleeve 3 only has 50mm, and when inner cylinder is pulled out from outer cylinder to greatest extent, the length of entire extension sleeve 3 will not
More than 100mm.
First beam collimator 2 is mounted on the outer end of the inner cylinder of extension sleeve 3, in use, the first beam collimator 2 passes through
Coupling optical fiber is connect with 785nm laser 1.
Band pass filter 4, convex lens 5 and axicon lens 6 are installed in the inner cylinder of extension sleeve 3, wherein band pass filter
4 are mounted in filter plate mounting base, and convex lens 5 and axicon lens 6 are mounted in lens mounting base, and the tip of axicon lens 6 is away from the
One beam collimator 2, convex lens 5 and axicon lens 6 can be plugged arbitrarily in lens mounting base, when what is installed in lens mounting base is
When convex lens 5, which can realize Raman spectrum imaging, when what is installed in lens mounting base is axicon lens 6, the optical path
System can realize deep layer spectral detection.
Dichroscope 7 is mounted in dichroscope fixed bin, and dichroscope fixed bin is mounted on the outer cylinder of extension sleeve 3
Outer end, the axis of dichroscope 7 and extension sleeve 3 are in 45 degree of angles, and reflective surface is towards extension sleeve 3.Dichroscope fixed bin
In cube shaped, the cube cassette that side length is 30mm can be made into.
Second beam collimator 8 is mounted at the reflected light light-emitting window of dichroscope fixed bin.Reflected light passes through the second light
Uniform collimated light beam is formed after beam collimator 8 is radiated at sample surfaces.
Imaging len 9 is mounted in lens fixed cylinder, and the transmitted light that lens fixed cylinder is mounted on dichroscope fixed bin goes out
At optical port.Lens fixed cylinder is cylindrical, can be made into the long cylinder of column that outer diameter is 25mm, length is 40mm.
Fiber optic bundle 10 is mounted on the other end of imaging len 9, the square battle array of optical fiber at the spectra collection end of fiber optic bundle 10
Column arrangement (shown in such as Fig. 3 (a), 4 × 4mm of area2, number of fibers 400 × 400,10 μm of optical fiber core diameter, NA=0.6), it can be with
Complete acquisition tissue surface spectral information, the optical fiber at spectral detection end is rectangle array arrangement, and (such as Fig. 3 (b) is shown, area 2
×8mm2, number of fibers 200 × 800,10 μm of optical fiber core diameter, NA=0.6), the size of rectangular array depends on imaging
The width and height of the slit of spectrometer 13 further need exist for the size and deflection angle that are effectively matched one-dimensional scanning galvanometer 12.Light
Spectral image data collecting efficiency and system signal noise ratio can be improved in fine this arrangement.The length of fiber optic bundle 10 is most short can
Take 300mm.
Achromatic doublet is mounted on lens in fixed cylinder to 11, and lens are mounted on fiber optic bundle 10 to fixed cylinder
Spectral detection end is integrated with notch filter piece or long pass filter piece group between two lens.Lens are cylindrical to fixed cylinder, can
It is made into the long cylinder of column that outer diameter is 25mm, length is 100mm.
One-dimensional scanning galvanometer 12 is mounted in galvanometer fixed bin, and galvanometer fixed bin is mounted on lens to the another of fixed cylinder
End, one-dimensional scanning galvanometer 12 and achromatic doublet are in 45 degree of angles to 11 axis.Galvanometer fixed bin in cube shaped,
The positive square box that side length is 40mm can be made into.
The advantage of line scanning optical spectrum imaging is: quasi-confocal form can be used, it only can be very big with the cost of very little
Light spectrum image-forming speed is improved in degree, and on one wire due to laser power profile, it is possible to reduce sample thermal decomposition
Or a possibility that photochemical reaction.
We use line scan pattern at spectral detection end, realize quickly to screen and record to compose with high light and differentiate
Rate, the spectroscopic data collection compared with wide range segment limit.
When what is installed in lens mounting base is convex lens 5, referring to Fig.1, which can realize Raman spectrum imaging,
The process of light spectrum image-forming is specific as follows:
Use 785nm laser 1 as excitation light source, excitation beam (solid line) is successively through overcoupling optical fiber, the first light beam
Uniformly collimation circular excitation is formed after collimator 2, band pass filter 4, convex lens 5, dichroscope 7 and the second beam collimator 8
Hot spot is radiated at sample surfaces, and the circle for being radiated at sample surfaces may be implemented in focal length and spatial position by adjusting convex lens 5
Shape excites the size of hot spot to adjust, and the best effort distance of the light path system is 50-100mm, is radiated at the circle of sample surfaces
The optimum size (diameter) for exciting hot spot is 2-3mm, and the anisotropic scattering light (dotted line) at sample surfaces different location is successively
After the second beam collimator 8, dichroscope 7 and imaging len 9, complete imaging is in the spectra collection end of fiber optic bundle 10, institute
At picture achromatic doublet is conducted to 11 by fiber optic bundle 10, achromatic doublet is between 11 two lens
It is integrated with notch filter piece (or long pass filter piece group), it is one-dimensional after notch filter piece (or long pass filter piece group) filtering Rayleigh scattering
The Raman diffused light of space different location is projeced into imaging spectrometer 13 by scanning galvanometer 12, is believed by imaging spectrometer 13 spectrum
Breath is recorded and is analyzed.
When what is installed in lens mounting base is axicon lens 6, referring to Fig. 2, which can be realized based on reverse space
Deviate the detection side Raman spectrum (Inverse Spatially Offset Raman Spectroscopy, Inverse SORS)
The deep layer spectral detection of method, spectral detection process are specific as follows:
Use 785nm laser 1 as excitation light source, excitation beam is successively through overcoupling optical fiber, the first beam collimator
2, uniformly collimation annular excitation hot spot is formed after band pass filter 4, axicon lens 6, dichroscope 7 and the second beam collimator 8 to shine
It penetrates in sample surfaces, (according to the difference of the degree of axicon lens 6, excites ring-shaped light by replacing the axicon lens 6 with different degrees
The range of choice of spatial offset between spot and dotted spectra collection area is also different, for example, when the degree of axicon lens 6 is 5 °
When, the peak excursion distance of the light path system is 10mm), the position of adjustment axicon lens 6 (passes through the inner cylinder of pull extension sleeve 3
To realize) and the distance (operating distance) that the second beam collimator 8 arrives working face is adjusted, it may be implemented to be radiated at sample surfaces
Annular excitation hot spot size adjusting, the radius (spatial offset Δ S) of ring-shaped light spot controllably, the light path system it is best
Operating distance is 10-100mm, and the optimum size (diameter) for being radiated at the annular excitation hot spot of sample surfaces is 4-6mm.According to light
Sub- transport theory, dotted spectra collection area can receive the optical information in spectral information acquisition zone (depth about 0.5-2mm)
Collection, and analyzed by the conduction of fiber optic bundle 10 to imaging spectrometer 13, thus, by controlling the consecutive variations of spatial offset,
Detect the Raman spectral information at sample different depth position, i.e. deep layer spectral detection function.
By combining organism optical model and existing in vitro slice Raman spectrum experimental result, establishing and improving theoretical mould
Intend method, contacting between theoretical description spatial offset Δ S and Inverse SORS detection, collection Raman light provided by the invention
Spectrum fast imaging and deep layer spectrum are quickly detected on integrated portable light path system and may be implemented normally to give birth under condition of living body
The experiment of the deep layer material composition and structural information of object tissue and canceration biological tissue detects.
It should be noted that the above embodiments do not limit the invention in any form, it is all to use equivalent replacement or equivalent change
The mode changed technical solution obtained, falls within the scope of protection of the present invention.
Claims (10)
1. a kind of collection Raman spectrum fast imaging and deep layer spectrum are quickly detected on integrated portable light path system, feature exists
In, comprising: the first beam collimator (2), extension sleeve (3), band pass filter (4), convex lens (5), axicon lens (6), two to
Look mirror (7), the second beam collimator (8), imaging len (9), fiber optic bundle (10), achromatic doublet are to (11) and one-dimensional
Scanning galvanometer (12), in which:
Extension sleeve (3) is made of inner barrel and outer barrel two parts, and inner cylinder can slidably reciprocate in outer cylinder along axial direction；
First beam collimator (2) is mounted on the outer end of the inner cylinder of extension sleeve (3)；
Band pass filter (4), convex lens (5) and axicon lens (6) are installed in the inner cylinder of extension sleeve (3), wherein axicon lens
(6) tip can arbitrarily plug in extension sleeve (3) away from the first beam collimator (2), convex lens (5) and axicon lens (6)；
Dichroscope (7) is mounted in dichroscope fixed bin, and dichroscope fixed bin is mounted on the outer cylinder of extension sleeve (3)
Outer end, the axis of dichroscope (7) and extension sleeve (3) are in 45 degree of angles, and reflective surface is towards extension sleeve (3)；
Second beam collimator (8) is mounted at the reflected light light-emitting window of dichroscope fixed bin；
Imaging len (9) is mounted in lens fixed cylinder, and the transmitted light that lens fixed cylinder is mounted on dichroscope fixed bin goes out light
At mouthful；
Fiber optic bundle (10) is mounted on the other end of imaging len (9), and the optical fiber at the spectra collection end of fiber optic bundle (10) is square
The optical fiber of array arrangement, spectral detection end is rectangle array arrangement；
Achromatic doublet is mounted on lens in fixed cylinder to (11), and lens are mounted on fiber optic bundle (10) to fixed cylinder
Spectral detection end；
One-dimensional scanning galvanometer (12) is mounted in galvanometer fixed bin, and galvanometer fixed bin is mounted on lens to the other end of fixed cylinder,
One-dimensional scanning galvanometer (12) and achromatic doublet are in 45 degree of angles to the axis of (11).
2. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that it is provided with filter plate mounting base and lens mounting base in the inner cylinder of the extension sleeve (3), and
Inner cylinder is provided with the pick-and-place mouth for picking and placing convex lens and axicon lens in lens mounting base position.
3. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that the inner cylinder of the extension sleeve (3) is cylindrical, outer diameter 25mm, length 50mm, outer cylinder
It is cylindrical, outer diameter 30mm, length 50mm.
4. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that the dichroscope fixed bin is in cube shaped, side length 30mm.
5. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that the lens fixed cylinder is cylindrical, outer diameter 25mm, length 40mm.
6. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that the arrangement size of the optical fiber at the spectral detection end of the fiber optic bundle (10) depends on imaging spectrometer
(13) width and height of slit further need exist for the size and deflection angle that are effectively matched one-dimensional scanning galvanometer (12).
7. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that it is 300mm that the length of the fiber optic bundle (10) is most short.
8. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that the achromatic doublet to be integrated between two lens of (11) notch filter piece or
Long pass filter piece group.
9. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable light
Road system, which is characterized in that the lens are in long tubular, outer diameter 25mm, length 100mm to fixed cylinder.
10. collection Raman spectrum fast imaging according to claim 1 and deep layer spectrum are quickly detected on integrated portable
Light path system, which is characterized in that the galvanometer fixed bin is in cube shaped, side length 40mm.
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| CN111044500A (en) * | 2019-12-30 | 2020-04-21 | 北京华泰诺安探测技术有限公司 | Raman probe capable of adjusting annular detection area |
| CN112326585A (en) * | 2020-10-28 | 2021-02-05 | 南京农业大学 | Infrared spectrum device and detection method for rapidly detecting powdery mildew of strawberries |
| CN113176249A (en) * | 2021-05-20 | 2021-07-27 | 中国科学技术大学 | Quick Raman scanning imager |
| CN115120233A (en) * | 2021-03-29 | 2022-09-30 | 上海近观科技有限责任公司 | Non-invasive biological characteristic signal detection device, system and method |
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