CN110499236B - Continuous flow type PCR device - Google Patents
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- CN110499236B CN110499236B CN201910893761.7A CN201910893761A CN110499236B CN 110499236 B CN110499236 B CN 110499236B CN 201910893761 A CN201910893761 A CN 201910893761A CN 110499236 B CN110499236 B CN 110499236B
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- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 28
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 230000001351 cycling effect Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 28
- 238000000137 annealing Methods 0.000 description 16
- 238000000034 method Methods 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 5
- 238000005286 illumination Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 4
- 239000012070 reactive reagent Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000004804 winding Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 1
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- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The application discloses a continuous flow PCR device, which comprises a liquid drop generating assembly, a temperature circulating assembly and a collecting assembly; the liquid drop generating assembly comprises a plurality of closed containers and a plurality of connecting pipes, one end of each connecting pipe corresponds to one closed container, and the other end of each connecting pipe is communicated in a converging manner; the liquid drop generating assembly is used for generating a plurality of liquid drops of mutually isolated reaction reagents; the temperature circulating assembly comprises a heater and a circulating conduit, the circulating conduit is turned back for multiple times between two end points of a first path along the first path, and the first path repeatedly passes through a high-temperature area close to the heater and a low-temperature area far away from the heater; the input end of the circulating conduit is connected with one end of the connecting pipe which is converged and communicated; the collection assembly is used for collecting the reagent flowing out of the output end of the circulation conduit. The application improves the accuracy of the fluorescent signal detection and simultaneously improves the reaction efficiency.
Description
Technical Field
The present application relates to the field of life sciences, and more particularly, to a continuous flow PCR device.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased, and with the development of science and technology, the PCR technique is increasingly applied to the technical fields of archaeology, criminal investigation and other biosciences.
The continuous flow polymerase chain reaction technology which is most widely applied mainly realizes the temperature cycle process by controlling the reciprocating motion of reaction reagents in heating areas of a plurality of constant temperature heating sheets. The temperatures of several thermostatically heated plates correspond to the annealing, extension and denaturation temperatures required for the polymerase chain reaction, respectively. Compared with the conventional Peltier temperature rise and fall method, the technology has the greatest advantage of realizing the rapid temperature rise and fall process, thereby greatly reducing the reaction time. However, the technology also has the problems that the two ends of the pipeline are far away, and the reaction reagent generally flows in the pipeline in a one-way mode, so that when the DNA generation amount is detected, because the illumination intensity of exciting light received at different positions is different, the liquid drop of the reaction reagent which is not sufficiently illuminated emits light, namely, the detection accuracy of a fluorescence signal is poor, and in addition, because the reaction reagent passes through a heating area in a one-way and repeated mode, the temperature is higher, the annealing temperature and the catch-of-eye temperature are not constant in the whole reaction process, and the reaction efficiency is greatly reduced.
Therefore, how to solve the problems of poor detection accuracy of fluorescent signals due to uneven light reception and low reaction efficiency due to uneven temperature is an urgent problem to be solved by those skilled in the art.
Content of application
The application aims to provide a continuous flow type PCR device to solve the problems of poor detection accuracy of fluorescence signals and low reaction efficiency in the prior art.
In order to solve the above technical problems, the present application provides a continuous flow PCR device, comprising a droplet generating assembly, a temperature cycling assembly, and a collecting assembly;
the liquid drop generating assembly comprises a plurality of closed containers and a plurality of connecting pipes, one end of each connecting pipe corresponds to one closed container, and the other end of each connecting pipe is communicated in a converging manner; the liquid drop generating assembly is used for generating a plurality of liquid drops of mutually isolated reaction reagents;
the temperature circulating assembly comprises a heater and a circulating conduit, the circulating conduit is turned back for multiple times between two end points of a first path along the first path, and the first path repeatedly passes through a high-temperature area close to the heater and a low-temperature area far away from the heater;
the input end of the circulating conduit is connected with one end of the connecting pipe which is converged and communicated;
the collection assembly is used for collecting the reagent flowing out of the output end of the circulation conduit.
Optionally, in the continuous flow PCR device, the temperature cycling assembly comprises a heat resistant rod on which the cycling conduit is wound;
the heater is arranged on one side of the heat resistance rod.
Optionally, in the continuous flow PCR device, the heat resistant rod is made of silica gel doped with copper powder.
Optionally, in the continuous flow PCR device, the first path lies within a first plane.
Optionally, in the continuous flow PCR device, the number of the heaters is one.
Optionally, in the continuous flow PCR device, the closed container is a syringe.
Optionally, in the continuous flow PCR device, the temperature range of the high temperature region is 93 to 97 degrees celsius, and the temperature range of the low temperature region is 60 to 67 degrees celsius, inclusive.
Optionally, in the continuous flow PCR device, the droplet generation assembly includes two closed containers, namely an oil phase container and a water phase container;
the water phase container is used for containing the reaction reagent;
the oil phase container is used for containing an oily isolating agent, and the oily isolating agent is used for isolating adjacent droplets of the reaction reagent.
Optionally, in the continuous flow PCR device, the oily release agent is a fluorinated oil.
Optionally, in the continuous flow PCR device, the droplets of the reaction reagents repeatedly pass through the circulation conduit at the high-temperature zone and the low-temperature zone 20 to 100 times, inclusive.
The continuous flow PCR device provided by the application comprises a liquid drop generating assembly, a temperature circulating assembly and a collecting assembly; the liquid drop generating assembly comprises a plurality of closed containers and a plurality of connecting pipes, one end of each connecting pipe corresponds to one closed container, and the other end of each connecting pipe is communicated in a converging manner; the liquid drop generating assembly is used for generating a plurality of liquid drops of mutually isolated reaction reagents; the temperature circulating assembly comprises a heater and a circulating conduit, the circulating conduit is turned back for multiple times between two end points of a first path along the first path, and the first path repeatedly passes through a high-temperature area close to the heater and a low-temperature area far away from the heater; the input end of the circulating conduit is connected with one end of the connecting pipe which is converged and communicated; the collection assembly is used for collecting the reagent flowing out of the output end of the circulation conduit. According to the method, the circulating guide pipe is set to be in a shape of repeatedly turning back along the same path, so that the liquid drops of the reaction reagent with larger annealing times difference gather in the adjacent or similar guide pipes, and further, when illumination excitation is carried out, the liquid drops with low annealing times and the liquid drops with high annealing times exist in an area with sufficient illumination, so that the fluorescence emitted by the excited liquid drops is completely determined by the number of DNA molecules in the liquid drops, and the accuracy of fluorescence signal detection is greatly improved; in addition, because the more time of annealing, the higher drop of temperature can be followed the same route and turned back, consequently can form temperature convection between the pipeline (low temperature) of the drop of adjacent low annealing number of times and the drop (high temperature) of high annealing number of times, make wholly temperature on the first route tends to the average, and this application has better temperature stability promptly to promote reaction efficiency.
Drawings
For a clearer explanation of the embodiments or technical solutions of the prior art of the present application, the drawings needed for the description of the embodiments or prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of one embodiment of a continuous flow PCR apparatus provided herein;
FIG. 2 is a schematic structural diagram of another embodiment of the continuous flow PCR device provided herein;
FIG. 3 is a liquid flow diagram in the circulation conduit of another embodiment of the continuous flow PCR device provided herein;
FIG. 4 is a partial schematic structural view of a continuous flow PCR apparatus according to still another embodiment of the present invention.
Detailed Description
In order that those skilled in the art will better understand the disclosure, the following detailed description will be given with reference to the accompanying drawings. It is to be understood that the embodiments described are only a few embodiments of the present application and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
At the heart of the present application, a continuous flow PCR device is provided, one embodiment of which is schematically illustrated in fig. 1, and comprises a droplet generation assembly 100, a temperature cycling assembly 200, and a collection assembly 300;
the liquid drop generating assembly 100 comprises a plurality of closed containers 101 and a plurality of connecting pipes 102, wherein one ends of the connecting pipes 102 correspond to the closed containers 101 one by one, and the other ends of the connecting pipes are converged and communicated; the droplet generation assembly 100 is used for generating a plurality of mutually isolated droplets of reactive reagents;
the temperature circulating assembly 200 includes a heater 202 and a circulating duct 201, the circulating duct 201 being turned back a plurality of times between both ends of a first path along the first path, the first path repeatedly passing through a high temperature region near the heater 202 and a low temperature region far from the heater 202;
the input end of the circulation conduit 201 is connected with one end of the connecting pipe 102 which is communicated in a converging way;
the collection assembly 300 is used to collect the reagent flowing out of the output end of the circulation conduit 201.
It should be noted that in fig. 1, the circulation duct 201 is wound around the heater 202, but the heater 202 is not located at the center of the winding of the circulation duct 201, and the circulation duct 201 is wound along an oval line perpendicular to the paper surface, thereby forming the high temperature region and the low temperature region.
Further, the number of the heaters 202 is one. This application can make through changing first route the circulation pipe 201 is close to and keeps away from heater 202 many times to accomplish the step of annealing, extension and degeneration, consequently need not polylith heater 202, a heater 202 just can satisfy the reaction demand, has avoided setting up a plurality of heaters 202 and a plurality of control circuit that carry out thermostatic control to heater 202, has simplified the system greatly, has simplified production technology, the cost is reduced.
Particularly, the temperature range of the high-temperature region is 93-97 ℃, and the temperature range of the low-temperature region is 60-67 ℃, including the end points;
in addition, the droplets of the reaction reagent repeatedly pass through the circulation duct 201 in the high-temperature region and the low-temperature region 20 to 100 times, inclusive. The parameter range is a range with better effect obtained after a large number of theoretical calculations and actual tests, and can be correspondingly improved according to actual circumstances.
It should be noted that the droplet generating assembly 100 includes two closed containers 101, which are an oil phase container and a water phase container;
the water phase container is used for containing the reaction reagent;
the oil phase container is used for containing an oily isolating agent, and the oily isolating agent is used for isolating adjacent droplets of the reaction reagent.
Further, the oily release agent is fluorinated oil, and the closed container 101 is a syringe. The fluorinated oil has excellent chemical stability and thermal stability, low viscosity and good lubricity, and is suitable for being used as a separant of a flow reaction; the injector has low cost and easy operation.
It should be noted that the first path only represents a path that the circulation duct 201 passes through, or is referred to as a shape of the circulation duct 201, and the circulation duct 201 of the present application may be regarded as a pipe that advances along a predetermined path between two end points, and turns back and forth several times when reaching one of the end points.
It should be noted that the terms "fold back", "back" and "forth" in the present application do not mean that the original path of the droplets is returned, but mean that the droplets continue along the circulation duct, but the shape of the circulation duct is folded back at the end point, and refer to the attached drawings.
The continuous flow PCR device provided in the present application comprises a droplet generation assembly 100, a temperature cycling assembly 200, and a collection assembly 300; the liquid drop generating assembly 100 comprises a plurality of closed containers 101 and a plurality of connecting pipes 102, wherein one ends of the connecting pipes 102 correspond to the closed containers 101 one by one, and the other ends of the connecting pipes are converged and communicated; the droplet generation assembly 100 is used for generating a plurality of mutually isolated droplets of reactive reagents; the temperature circulating assembly 200 includes a heater 202 and a circulating duct 201, the circulating duct 201 being turned back a plurality of times between both ends of a first path along the first path, the first path repeatedly passing through a high temperature region near the heater 202 and a low temperature region far from the heater 202; the input end of the circulation conduit 201 is connected with one end of the connecting pipe 102 which is communicated in a converging way; the collection assembly 300 is used to collect the reagent flowing out of the output end of the circulation conduit 201. According to the method, the circulating conduit 201 is set to be in a shape of being repeatedly folded back along the same path, so that the liquid drops of the reaction reagents with larger annealing times difference gather in the adjacent or similar conduits, and further, when illumination excitation is carried out, the liquid drops with low annealing times and the liquid drops with high annealing times exist in an area with sufficient illumination, so that the fluorescence emitted by the excited liquid drops is completely determined by the number of DNA molecules in the liquid drops, and the accuracy of fluorescence signal detection is greatly improved; in addition, because the more time of annealing, the higher drop of temperature can be followed the same route and turned back, consequently can form temperature convection between the pipeline (low temperature) of the drop of adjacent low annealing number of times and the drop (high temperature) of high annealing number of times, make wholly temperature on the first route tends to the average, and this application has better temperature stability promptly to promote reaction efficiency.
On the basis of the first embodiment, the arrangement of the circulation conduit 201 is limited to obtain a second embodiment, which is shown in fig. 2, and includes a droplet generation assembly 100, a temperature circulation assembly 200, and a collection assembly 300;
the liquid drop generating assembly 100 comprises a plurality of closed containers 101 and a plurality of connecting pipes 102, wherein one ends of the connecting pipes 102 correspond to the closed containers 101 one by one, and the other ends of the connecting pipes are converged and communicated; the droplet generation assembly 100 is used for generating a plurality of mutually isolated droplets of reactive reagents;
the temperature circulating assembly 200 includes a heater 202 and a circulating duct 201, the circulating duct 201 being turned back a plurality of times between both ends of a first path along the first path, the first path repeatedly passing through a high temperature region near the heater 202 and a low temperature region far from the heater 202;
the input end of the circulation conduit 201 is connected with one end of the connecting pipe 102 which is communicated in a converging way;
the collection assembly 300 is used for collecting the reagent flowing out from the output end of the circulation conduit 201;
the temperature cycling assembly 200 comprises a heat resistant rod 203, and the cycling conduit 201 is wound on the heat resistant rod 203;
the heater 202 is disposed at one side of the heat blocking rod 203.
The difference between this embodiment and the above embodiment is that the heat-resistant rod 203 is added in this embodiment and the circulation conduit 201 is wound around the heat-resistant rod 203, and the rest of the structure is the same as that of the above embodiment, and will not be described again.
In the present embodiment, the circulation conduit 201 is wound around the heat blocking rod 203, which facilitates the installation and fixation of the circulation conduit 201, simplifies the manufacturing process, and improves the production efficiency, and secondly, the heat blocking rod 203 can save the distance from the high temperature region to the low temperature region, thereby greatly reducing the space occupation of the continuous flow type PCR device, and making the installation and setting of the device more flexible and convenient.
The heat blocking rods 203 comprise cylindrical rods and also prismatic rods.
Furthermore, the heat resistant rod 203 is made of silica gel and copper powder. By changing the ratio of the copper powder to the silica gel, the thermal resistance of the thermal resistance rod 203 can be easily changed, and the first path can be conveniently adjusted according to actual conditions (such as different external temperatures or limited space).
For clarity of the flow direction of the droplets of the reaction reagent in the circulation conduit 201, refer to fig. 3.
The circulation duct 201 takes the form of a multi-spiral, for example, an even-numbered multi-spiral winding manner such as a double-spiral four-spiral six-spiral or the like, or an odd-numbered multi-spiral winding manner such as a three-spiral five-spiral seven-spiral or the like, and thereby the annealing temperature, the space temperature of the extension temperature region, the stability and the balance are improved.
On the basis of the first embodiment, the arrangement of the circulation conduit 201 may be further limited, and a third embodiment is obtained, a schematic partial structure of which is shown in fig. 4 and includes a droplet generation assembly 100, a temperature circulation assembly 200, and a collection assembly 300;
the liquid drop generating assembly 100 comprises a plurality of closed containers 101 and a plurality of connecting pipes 102, wherein one ends of the connecting pipes 102 correspond to the closed containers 101 one by one, and the other ends of the connecting pipes are converged and communicated; the droplet generation assembly 100 is used for generating a plurality of mutually isolated droplets of reactive reagents;
the temperature circulating assembly 200 includes a heater 202 and a circulating duct 201, the circulating duct 201 being turned back a plurality of times between both ends of a first path along the first path, the first path repeatedly passing through a high temperature region near the heater 202 and a low temperature region far from the heater 202;
the input end of the circulation conduit 201 is connected with one end of the connecting pipe 102 which is communicated in a converging way;
the collection assembly 300 is used for collecting the reagent flowing out from the output end of the circulation conduit 201;
the first path lies in a first plane.
The difference between the present embodiment and the above embodiments is that the present embodiment defines the arrangement of the circulation duct 201 as a two-dimensional arrangement, and the rest of the structure is the same as the above embodiments, and will not be described herein again.
The specific embodiment is another shape of the first path, namely, the obtained plate-shaped temperature cycle component 200, the component only occupies space in the plane direction, and can meet the requirements of various conditions, a larger area is fully excited when the component is irradiated by excitation light, the observation result is more visual and obvious, and the fluorescence detection accuracy can be further improved.
In the plate-shaped temperature circulating assembly 200, the circulating pipe 201 reciprocates between both ends of the first path in a serpentine cycle.
The embodiments are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same or similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
It is to be noted that, in the present specification, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The continuous flow PCR device provided in the present application is described in detail above. The principles and embodiments of the present application are explained herein using specific examples, which are provided only to help understand the method and the core idea of the present application. It should be noted that, for those skilled in the art, it is possible to make several improvements and modifications to the present application without departing from the principle of the present application, and such improvements and modifications also fall within the scope of the claims of the present application.
Claims (9)
1. A continuous flow PCR device is characterized by comprising a liquid drop generating component, a temperature circulating component and a collecting component;
the liquid drop generating assembly comprises a plurality of closed containers and a plurality of connecting pipes, one end of each connecting pipe corresponds to one closed container, and the other end of each connecting pipe is communicated in a converging manner; the liquid drop generating assembly is used for generating a plurality of liquid drops of mutually isolated reaction reagents;
the temperature circulating assembly comprises a heater and a circulating conduit, the circulating conduit is turned back for multiple times between two end points of a first path along the first path, and the first path repeatedly passes through a high-temperature area close to the heater and a low-temperature area far away from the heater;
the input end of the circulating conduit is connected with one end of the connecting pipe which is converged and communicated;
the collection assembly is used for collecting the reagent flowing out of the output end of the circulation conduit;
the temperature cycling assembly comprises a heat resistance bar on which the cycling conduit is wound;
the heater is arranged on one side of the heat resistance rod.
2. The continuous flow PCR device of claim 1, wherein the heat bars are doped with silica gel and copper powder.
3. The continuous flow PCR device of claim 1, wherein the first path lies within a first plane.
4. The continuous flow PCR device of claim 1, wherein the number of heaters is one.
5. The continuous flow PCR device of claim 1, wherein the high temperature zone has a temperature range of 93 degrees celsius to 97 degrees celsius and the low temperature zone has a temperature range of 60 degrees celsius to 67 degrees celsius, inclusive.
6. The continuous flow PCR device of claim 1, wherein the droplet generation assembly comprises two closed containers, an oil phase container and a water phase container;
the water phase container is used for containing the reaction reagent;
the oil phase container is used for containing an oily isolating agent, and the oily isolating agent is used for isolating adjacent droplets of the reaction reagent.
7. The continuous-flow PCR device of claim 6, wherein the oily release agent is a fluorinated oil.
8. The continuous flow PCR device of claim 1, wherein the closed container is a syringe.
9. The continuous-flow PCR device according to any one of claims 1 to 8, wherein the droplets of the reaction reagents repeatedly pass through the circulation conduit at the high-temperature zone and the low-temperature zone 20 to 100 times, inclusive.
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| CN201910893761.7A CN110499236B (en) | 2019-09-20 | 2019-09-20 | Continuous flow type PCR device |
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| CN201910893761.7A CN110499236B (en) | 2019-09-20 | 2019-09-20 | Continuous flow type PCR device |
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| CN110499236B true CN110499236B (en) | 2020-12-08 |
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| CN113862140B (en) * | 2021-09-27 | 2024-04-09 | 广东省科学院健康医学研究所 | Portable liquid drop digital PCR device |
| CN114653411A (en) * | 2022-03-04 | 2022-06-24 | 广东省科学院生物与医学工程研究所 | Three-dimensional spiral chip |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5720923A (en) * | 1993-07-28 | 1998-02-24 | The Perkin-Elmer Corporation | Nucleic acid amplification reaction apparatus |
| JP2007300896A (en) * | 2006-05-15 | 2007-11-22 | Ishikawa Pref Gov | Device and method for quantifying gene |
| CN107164523A (en) * | 2017-06-27 | 2017-09-15 | 中国科学院长春光学精密机械与物理研究所 | A kind of method and device for digital pcr |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5720923A (en) * | 1993-07-28 | 1998-02-24 | The Perkin-Elmer Corporation | Nucleic acid amplification reaction apparatus |
| JP2007300896A (en) * | 2006-05-15 | 2007-11-22 | Ishikawa Pref Gov | Device and method for quantifying gene |
| CN107164523A (en) * | 2017-06-27 | 2017-09-15 | 中国科学院长春光学精密机械与物理研究所 | A kind of method and device for digital pcr |
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