CN110499236A - A kind of continuous-flow PCR device - Google Patents
A kind of continuous-flow PCR device Download PDFInfo
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- CN110499236A CN110499236A CN201910893761.7A CN201910893761A CN110499236A CN 110499236 A CN110499236 A CN 110499236A CN 201910893761 A CN201910893761 A CN 201910893761A CN 110499236 A CN110499236 A CN 110499236A
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 31
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 30
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 28
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 27
- 238000000137 annealing Methods 0.000 description 15
- 238000000034 method Methods 0.000 description 14
- 238000005286 illumination Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011840 criminal investigation Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
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- Life Sciences & Earth Sciences (AREA)
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Abstract
This application discloses a kind of continuous-flow PCR devices, including drop formation component, temperature cycles component and collection assembly；The drop formation component includes multiple closed containers and multiple connecting tubes, and one end of multiple connecting tubes and the closed container correspond, and the other end converges connection；The drop formation component is used to generate the drop of multiple mutually isolated reaction reagents；The temperature cycles component includes heater and circulation conduit, the circulation conduit is along first path, it turns back between the two-end-point of the first path repeatedly, the first path passes through the high-temperature area close to the heater and the low-temperature region far from the heater repeatedly；The input terminal of the circulation conduit is connected with one end that the connecting tube converges connection；The collection assembly is used to collect the reagent flowed out from the output end of the circulation conduit.The application improves the accuracy rate of the fluorescence signal detection, while also improving reaction efficiency.
Description
Technical field
This application involves life sciences, more particularly to a kind of continuous-flow PCR device.
Background technique
Polymerase chain reaction (PCR) is a kind of for amplifying the Protocols in Molecular Biology for expanding specific DNA fragmentation, it can
Regard the special DNA replication dna of in vitro as, the maximum feature of PCR is that micro DNA can be significantly increased, with the hair of science and technology
Exhibition, round pcr are applied to archaeology, criminal investigation and other biological science and technology field more and more.
Wherein, most widely used continuous flowing type polymerase chain reaction technique mainly passes through control reaction reagent several
The heating region of heated at constant temperature piece moves back and forth to realize temperature cycles process.The temperature of several heated at constant temperature pieces corresponds respectively to
Annealing, extension required for polymerase chain reaction and denaturation temperature.Relative to the current Peltier heating and cooling side routinely used
Method, this technology biggest advantage can be achieved on the process of rapid temperature rise and drop, to substantially reduce the reaction time.But the skill
Equally there are also problems for art, i.e., pipe ends are apart from each other, and reaction reagent is usually one-way flow in the duct, to make
When must detect DNA production quantity, since the exciting light intensity of illumination that different location is subject to is different, cause to be illuminated by the light insufficient reaction
Reagent droplet shines insufficient, i.e. fluorescence signal accuracy in detection is poor, further, since reaction reagent is unidirectionally repeatedly by heating zone
Domain, leads to more to arrive that subsequent temperature is higher, and the annealing temperature and expression in the eyes temperature in entire reaction process are non-constant, substantially reduce anti-
Answer efficiency.
Therefore, it is poor and since temperature unevenness is led how to solve the fluorescence signal accuracy in detection as caused by light is uneven
The low problem of the reaction efficiency of cause is those skilled in the art's urgent problem to be solved.
Apply for content
The purpose of the application is to provide a kind of continuous-flow PCR device, is detected with solving fluorescence signal in the prior art
The problem of poor accuracy, simultaneous reactions low efficiency.
In order to solve the above technical problems, the application provides a kind of continuous-flow PCR device, including drop formation component,
Temperature cycles component and collection assembly；
The drop formation component includes multiple closed containers and multiple connecting tubes, one end of multiple connecting tubes and institute
Closed container one-to-one correspondence is stated, the other end converges connection；The drop formation component is for generating multiple mutually isolated reactions
The drop of reagent；
The temperature cycles component includes heater and circulation conduit, and the circulation conduit is along first path, described
It turns back between the two-end-point in one path repeatedly, the first path is passed through repeatedly close to the high-temperature area of the heater and far from institute
State the low-temperature region of heater；
The input terminal of the circulation conduit is connected with one end that the connecting tube converges connection；
The collection assembly is used to collect the reagent flowed out from the output end of the circulation conduit.
Optionally, in the continuous-flow PCR device, the temperature cycles component includes thermal resistance stick, described to follow
Ring conduit is wrapped on the thermal resistance stick；
The side of the thermal resistance stick is arranged in the heater.
Optionally, in the continuous-flow PCR device, the thermal resistance stick is resistance made of silica gel and copper powder doping
Hot pin.
Optionally, in the continuous-flow PCR device, the first path is located in the first plane.
Optionally, in the continuous-flow PCR device, the quantity of the heater is one.
Optionally, in the continuous-flow PCR device, the closed container is syringe.
Optionally, in the continuous-flow PCR device, the temperature range of the high-temperature region is 93 degrees Celsius to 97
Degree Celsius, the temperature range of the low-temperature space is 60 degrees Celsius to 67 degrees Celsius, including endpoint value.
Optionally, in the continuous-flow PCR device, the drop formation component includes two closed containers,
Respectively oily phase container and water phase container；
The water phase container is for holding the reaction reagent；
The oil phase container is used to be isolated adjacent reaction examination for holding oiliness interleaving agent, the oiliness interleaving agent
The drop of agent.
Optionally, in the continuous-flow PCR device, the oiliness interleaving agent is fluorinated oil.
Optionally, in the continuous-flow PCR device, the drop of the reaction reagent is in the circulation conduit
20 times to 100 times, including endpoint value are iterated through in the high-temperature region and the low-temperature space.
Continuous-flow PCR device provided herein, including drop formation component, temperature cycles component and collection group
Part；The drop formation component includes multiple closed containers and multiple connecting tubes, one end of multiple connecting tubes with it is described close
Container one-to-one correspondence is closed, the other end converges connection；The drop formation component is for generating multiple mutually isolated reaction reagents
Drop；The temperature cycles component includes heater and circulation conduit, and the circulation conduit is along first path, described first
It turns back between the two-end-point in path repeatedly, the first path is passed through repeatedly close to the high-temperature area of the heater and far from described
The low-temperature region of heater；The input terminal of the circulation conduit is connected with one end that the connecting tube converges connection；The collection
Component is used to collect the reagent flowed out from the output end of the circulation conduit.The application is by setting edge for the circulation conduit
The shape that same path is turned back repeatedly, make by annealing times differ the drop of biggish reaction reagent and be gathered in phase
In adjacent or similar conduit, so that existing simultaneously low annealing time in the sufficient region of illumination when carrying out illumination excitation
The drop of several drop and high annealing times, the fluorescence for guaranteeing that drop issues after being excited are divided by the DNA of drop internal completely
Subnumber determines, substantially increases the accuracy rate of the fluorescence signal detection；Further, since by relatively repeatedly annealing, temperature are higher
Drop can turn back along identical path, therefore the pipeline (low temperature) of the drop of adjacent low annealing times and high annealing times
It will form temperature convection between drop (high temperature), so that the temperature in the entire first path is tended to be average, i.e., the application has more
Good temperature stability, to promote reaction efficiency.
Detailed description of the invention
It, below will be to embodiment or existing for the clearer technical solution for illustrating the embodiment of the present application or the prior art
Attached drawing needed in technical description is briefly described, it should be apparent that, the accompanying drawings in the following description is only this Shen
Some embodiments please for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is a kind of structural schematic diagram of specific embodiment of continuous-flow PCR device provided by the present application；
Fig. 2 is the structural schematic diagram of another specific embodiment of continuous-flow PCR device provided by the present application；
Fig. 3 is in the circulation conduit of another specific embodiment of continuous-flow PCR device provided by the present application
Liquid flow graph；
Fig. 4 is the partial structurtes signal of another specific embodiment of continuous-flow PCR device provided by the present application
Figure.
Specific embodiment
In order to make those skilled in the art more fully understand application scheme, with reference to the accompanying drawings and detailed description
The application is described in further detail.Obviously, described embodiments are only a part of embodiments of the present application, rather than
Whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making creative work premise
Under every other embodiment obtained, shall fall in the protection scope of this application.
The core of the application is to provide a kind of continuous-flow PCR device, a kind of structural representation of specific embodiment
Figure is as shown in Figure 1, include drop formation component 100, temperature cycles component 200 and collection assembly 300；
The drop formation component 100 includes multiple closed containers 101 and multiple connecting tubes 102, multiple connecting tubes
102 one end and the closed container 101 correspond, and the other end converges connection；The drop formation component 100 is for generating
The drop of multiple mutually isolated reaction reagents；
The temperature cycles component 200 includes heater 202 and circulation conduit 201, and the circulation conduit 201 is along the first via
Diameter is turned back repeatedly between the two-end-point of the first path, and the first path passes through the height close to the heater 202 repeatedly
The low-temperature region of temperature area and the separate heater 202；
The input terminal of the circulation conduit 201 is connected with one end that the connecting tube 102 converges connection；
The collection assembly 300 is used to collect the reagent flowed out from the output end of the circulation conduit 201.
It should be noted that circulation conduit 201 described in Fig. 1 is wrapped on the heater 202, but the heater
202 in the center of circle of the circulation conduit 201 winding, and the circulation conduit 201 is surround along the oval route of vertical paper, with this
Form the high-temperature area and the low-temperature region.
Further, the quantity of the heater 202 is one.The application can be made by changing the first path
The circulation conduit more than the 201 times close and separate heaters 202, thus the step of completing annealing, extending and be denaturalized, therefore
Do not need muti-piece heater 202, one piece of heater 202 is just able to satisfy reaction requirement, avoid the multiple heaters 202 of setting and
Multiple pairs of heaters 202 carry out thermostatically controlled control circuit, enormously simplify system, simplify production technology, reduce into
This.
Particularly, the temperature range of the high-temperature region is 93 degrees Celsius to 97 degrees Celsius, the temperature range of the low-temperature space
For 60 degrees Celsius to 67 degrees Celsius, including endpoint value；
In addition, the drop of the reaction reagent is anti-in the high-temperature region and the low-temperature space in the circulation conduit 201
Again by 20 times to 100 times, including endpoint value.Above parameter range is obtained after a large amount of theoretical calculations and practice examining
The preferable range of effect can also make corresponding improve according to practical circumstances certainly.
It should be noted that the drop formation component 100 includes two closed containers 101, respectively oily phase container and
Water phase container；
The water phase container is for holding the reaction reagent；
The oil phase container is used to be isolated adjacent reaction examination for holding oiliness interleaving agent, the oiliness interleaving agent
The drop of agent.
Further, the oiliness interleaving agent is fluorinated oil, and the closed container 101 is syringe.The change of fluorinated oil
It learns stability and thermal stability is outstanding, viscosity is low, and lubricity is good, is suitable as the interleaving agent of flowing reactive；Syringe is at low cost,
It is easy to operate.
It should be noted that the first path only represents the path that the circulation conduit 201 passes through, or referred to as institute
The shape of circulation conduit 201 is stated, the circulation conduit 201 of the application can be regarded as the pipe to advance between two-end-point along predefined paths
Road is just turned back to one of endpoint, is repeated several times.
It is significant to note that " turning back " " round-trip " " repeatedly " in the application does not imply that drop backtracking,
And refer to that drop is moved on along the circulating line, but the shape of the circulating line is turned back in endpoint, please specifically it join
Examine Figure of description.
Continuous-flow PCR device provided herein, including drop formation component 100, temperature cycles component 200 and
Collection assembly 300；The drop formation component 100 includes multiple closed containers 101 and multiple connecting tubes 102, multiple companies
One end of adapter tube 102 and the closed container 101 correspond, and the other end converges connection；The drop formation component 100 is used for
Generate the drop of multiple mutually isolated reaction reagents；The temperature cycles component 200 includes heater 202 and circulation conduit
201, the circulation conduit 201 is turned back repeatedly, the first path is anti-along first path between the two-end-point of the first path
The low-temperature region of the multiple high-temperature area by close to the heater 202 and the separate heater 202；The circulation conduit
201 input terminal is connected with one end that the connecting tube 102 converges connection；The collection assembly 300 is followed for collecting from described
The reagent of the output end outflow of ring conduit 201.The application is anti-by the way that the circulation conduit 201 to be set as carrying out along same path
The shape turned back again, make by annealing times differ the drop of biggish reaction reagent and be gathered in adjacent or similar conduit
In, so that existing simultaneously the drop of low annealing times when carrying out illumination excitation in the sufficient region of illumination and height moving back
The drop of fiery number, the fluorescence for guaranteeing that drop issues after being excited is determined by the DNA molecular number of drop internal completely, is mentioned significantly
The high accuracy rate of the fluorescence signal detection；Further, since by can be along identical compared with multiple annealing, the higher drop of temperature
Path is turned back, therefore meeting between the pipeline (low temperature) of the drop of adjacent low annealing times and the drop (high temperature) of high annealing times
Formation temperature convection current makes the temperature in the entire first path tend to be average, i.e., the application has better temperature stability, from
And promote reaction efficiency.
On the basis of specific embodiment one, the arrangement of the circulation conduit 201 is limited, specific embodiment party is obtained
Formula two, structural schematic diagram is as shown in Fig. 2, include drop formation component 100, temperature cycles component 200 and collection assembly 300；
The drop formation component 100 includes multiple closed containers 101 and multiple connecting tubes 102, multiple connecting tubes
102 one end and the closed container 101 correspond, and the other end converges connection；The drop formation component 100 is for generating
The drop of multiple mutually isolated reaction reagents；
The temperature cycles component 200 includes heater 202 and circulation conduit 201, and the circulation conduit 201 is along the first via
Diameter is turned back repeatedly between the two-end-point of the first path, and the first path passes through the height close to the heater 202 repeatedly
The low-temperature region of temperature area and the separate heater 202；
The input terminal of the circulation conduit 201 is connected with one end that the connecting tube 102 converges connection；
The collection assembly 300 is used to collect the reagent flowed out from the output end of the circulation conduit 201；
The temperature cycles component 200 includes thermal resistance stick 203, and the circulation conduit 201 is wrapped in the thermal resistance stick 203
On；
The side of the thermal resistance stick 203 is arranged in the heater 202.
Present embodiment and above-mentioned specific embodiment the difference is that, be additionally arranged in present embodiment
The circulation conduit 201 is simultaneously wrapped on the thermal resistance stick 203 by the thermal resistance stick 203, remaining structure with above-mentioned specific reality
It is identical to apply mode, it is not reinflated herein to repeat.
The circulation conduit 201 is wrapped on the thermal resistance stick 203 in present embodiment, one facilitates described
The installation and fixation of circulation conduit 201, simplify manufacture craft, improve production efficiency, second, utilizing the thermal resistance stick 203
The distance for being reduced to the low-temperature space to be passed through from the high-temperature region can be saved, the continuous-flow PCR dress is substantially reduced
The space hold set makes the more flexible convenience of the installation settings of device.
The thermal resistance stick 203 includes cylindrical bar, also includes rib stick.
Further, the thermal resistance stick 203 is that silica gel and copper powder adulterate manufactured thermal resistance stick 203.By changing copper powder
With the proportion of silica gel, it can easily change the thermal resistance of the thermal resistance stick 203, it is convenient that the first path is carried out according to the actual situation
Adjust (situations such as such as external temperature is different or space is limited).
For the flow direction of the drop of the reaction reagent in the apparent circulation conduit 201, Fig. 3 is please referred to.
The circulation conduit 201 takes the more spirals of even numbers such as multispiral form, such as four spiral of double helix, six spiral to twine
Around more spiral winding modes of the more spirals of odd numbers such as seven spirals of mode or three spirals, five spiral, and so as to improve annealing temperature, prolong
Stretch the space temperature of temperature region, stability and harmony.
On the basis of specific embodiment one, another restriction can be also carried out to the arrangement of the circulation conduit 201, is obtained
To specific embodiment three, partial structural diagram is as shown in figure 4, include drop formation component 100, temperature cycles component
200 and collection assembly 300；
The drop formation component 100 includes multiple closed containers 101 and multiple connecting tubes 102, multiple connecting tubes
102 one end and the closed container 101 correspond, and the other end converges connection；The drop formation component 100 is for generating
The drop of multiple mutually isolated reaction reagents；
The temperature cycles component 200 includes heater 202 and circulation conduit 201, and the circulation conduit 201 is along the first via
Diameter is turned back repeatedly between the two-end-point of the first path, and the first path passes through the height close to the heater 202 repeatedly
The low-temperature region of temperature area and the separate heater 202；
The input terminal of the circulation conduit 201 is connected with one end that the connecting tube 102 converges connection；
The collection assembly 300 is used to collect the reagent flowed out from the output end of the circulation conduit 201；
The first path is located in the first plane.
Present embodiment and above-mentioned specific embodiment the difference is that, defined in present embodiment
The arrangement of the circulation conduit 201 is two dimension arrangement, remaining structure is identical as above-mentioned specific embodiment, not reinflated herein
It repeats.
Present embodiment is another shape of the first path to get the plate temperature cycles component 200 arrived,
This component only occupied space in the in-plane direction, is suitable for a variety of situation needs, and when being shone by exciting light, has bigger
Area is sufficiently excited, and observed result is more intuitive obvious, can further improve the fluorescence detection accuracy rate.
In the plate temperature cycles component 200, the circulation conduit 201 moves in circles according to snakelike in described first
Between the two-end-point in path.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with it is other
The difference of embodiment, same or similar part may refer to each other between each embodiment.For being filled disclosed in embodiment
For setting, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is referring to method part
Explanation.
It should be noted that in the present specification, relational terms such as first and second and the like are used merely to one
A entity or operation with another entity or operate distinguish, without necessarily requiring or implying these entities or operation it
Between there are any actual relationship or orders.Moreover, the terms "include", "comprise" or its any other variant are intended to
Cover non-exclusive inclusion, so that the process, method, article or equipment for including a series of elements not only includes those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or setting
Standby intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in the process, method, article or apparatus that includes the element.
Continuous-flow PCR device provided herein is described in detail above.It is used herein specifically
The principle and implementation of this application are described for a example, the application that the above embodiments are only used to help understand
Method and its core concept.It should be pointed out that for those skilled in the art, not departing from the application original
Under the premise of reason, can also to the application, some improvement and modification can also be carried out, these improvement and modification also fall into the application right and want
In the protection scope asked.
Claims (10)
1. a kind of continuous-flow PCR device, which is characterized in that including drop formation component, temperature cycles component and collection group
Part；
The drop formation component includes multiple closed containers and multiple connecting tubes, one end of multiple connecting tubes with it is described close
Container one-to-one correspondence is closed, the other end converges connection；The drop formation component is for generating multiple mutually isolated reaction reagents
Drop；
The temperature cycles component includes heater and circulation conduit, and the circulation conduit is along first path, in the first via
It turns back between the two-end-point of diameter multiple, the first path adds by the high-temperature area close to the heater and far from described repeatedly
The low-temperature region of hot device；
The input terminal of the circulation conduit is connected with one end that the connecting tube converges connection；
The collection assembly is used to collect the reagent flowed out from the output end of the circulation conduit.
2. continuous-flow PCR device as described in claim 1, which is characterized in that the temperature cycles component includes thermal resistance
Stick, the circulation conduit are wrapped on the thermal resistance stick；
The side of the thermal resistance stick is arranged in the heater.
3. continuous-flow PCR device as claimed in claim 2, which is characterized in that the thermal resistance stick is that silica gel is mixed with copper powder
Thermal resistance stick made of miscellaneous.
4. continuous-flow PCR device as described in claim 1, which is characterized in that the first path is located at the first plane
It is interior.
5. continuous-flow PCR device as described in claim 1, which is characterized in that the quantity of the heater is one.
6. continuous-flow PCR device as described in claim 1, which is characterized in that the temperature range of the high-temperature region is 93
Degree Celsius to 97 degrees Celsius, the temperature range of the low-temperature space is 60 degrees Celsius to 67 degrees Celsius, including endpoint value.
7. continuous-flow PCR device as described in claim 1, which is characterized in that the drop formation component includes two
Closed container, respectively oily phase container and water phase container；
The water phase container is for holding the reaction reagent；
The oil phase container is for holding oiliness interleaving agent, and the oiliness interleaving agent is for being isolated the adjacent reaction reagent
Drop.
8. continuous-flow PCR device as described in claim 1, which is characterized in that the oiliness interleaving agent is fluorinated oil.
9. continuous-flow PCR device as described in claim 1, which is characterized in that the closed container is syringe.
10. continuous-flow PCR device as described in any one of claim 1 to 9, which is characterized in that the reaction reagent
Drop iterates through 20 times to 100 times, including endpoint value in the high-temperature region and the low-temperature space in the circulation conduit.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910893761.7A CN110499236B (en) | 2019-09-20 | 2019-09-20 | Continuous flow type PCR device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910893761.7A CN110499236B (en) | 2019-09-20 | 2019-09-20 | Continuous flow type PCR device |
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| CN110499236A true CN110499236A (en) | 2019-11-26 |
| CN110499236B CN110499236B (en) | 2020-12-08 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113862140A (en) * | 2021-09-27 | 2021-12-31 | 广东省科学院健康医学研究所 | Portable liquid drop digital PCR device |
| CN114653411A (en) * | 2022-03-04 | 2022-06-24 | 广东省科学院生物与医学工程研究所 | Three-dimensional spiral chip |
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| JP2007300896A (en) * | 2006-05-15 | 2007-11-22 | Ishikawa Pref Gov | Device and method for quantifying gene |
| CN107164523A (en) * | 2017-06-27 | 2017-09-15 | 中国科学院长春光学精密机械与物理研究所 | A kind of method and device for digital pcr |
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2019
- 2019-09-20 CN CN201910893761.7A patent/CN110499236B/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5720923A (en) * | 1993-07-28 | 1998-02-24 | The Perkin-Elmer Corporation | Nucleic acid amplification reaction apparatus |
| JP2007300896A (en) * | 2006-05-15 | 2007-11-22 | Ishikawa Pref Gov | Device and method for quantifying gene |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113862140A (en) * | 2021-09-27 | 2021-12-31 | 广东省科学院健康医学研究所 | Portable liquid drop digital PCR device |
| CN113862140B (en) * | 2021-09-27 | 2024-04-09 | 广东省科学院健康医学研究所 | Portable liquid drop digital PCR device |
| CN114653411A (en) * | 2022-03-04 | 2022-06-24 | 广东省科学院生物与医学工程研究所 | Three-dimensional spiral chip |
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