CN110498845A - Peste des petits ruminants diagnostic kit - Google Patents
Peste des petits ruminants diagnostic kit Download PDFInfo
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- CN110498845A CN110498845A CN201810480848.7A CN201810480848A CN110498845A CN 110498845 A CN110498845 A CN 110498845A CN 201810480848 A CN201810480848 A CN 201810480848A CN 110498845 A CN110498845 A CN 110498845A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
- C12N2760/18422—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/115—Paramyxoviridae, e.g. parainfluenza virus
- G01N2333/12—Mumps virus; Measles virus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
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Abstract
The present invention relates to peste des petits ruminants diagnostic kits.Detection kit of the invention includes substrate, and particular polypeptide or the particular polypeptide combination being independently connected in the substrate.
Description
Technical field
The invention mainly relates to diagnostic kit for animals and diagnostic methods.Specifically, the present invention relates to detections pair
As biological source biological sample in the presence or absence of anti-PPR virus antibody (IgG) kit.
Background technique
Peste des petits ruminants (Pestedespetitsruminants, PPR) is by PPR virus
One kind caused by (Pestedespetitsruminantsvirus, PPRV) is acute, strong, contagious disease, because it is higher
Morbidity and mortality, to the animals such as goat, sheep, white-tailed deer, wild gazelle sheep and Tibetan antelope have greatly threaten,
It is the deadly infectious disease generally acknowledged in world wide, which is also classified as a kind of zoonosis by China.The section of nineteen forty-two, West Africa are special
After Di Wa reports peste des petits ruminants for the first time, the most of country in Africa reported the occurrence of this disease in succession.In recent years, small to ruminate beast
Epidemic disease is in further spread of trend.China in 2007 finds that this disease is passed to China Tibet locally from India by border for the first time
Area causes some areas popular, seriously threatens to especially small safe and healthy constitute for ruminating beast of China's animal health safety,
Therefore, the foundation of peste des petits ruminants diagnostic method of high sensitivity, high specificity just seems especially urgent.
Small epizootic disease viral (PPRV) of instead working as belongs to Paramyxoviridae (Paramyxoviriade) Morbillivirus
(Morbollivirus), other members belonged to have rinderpest virus (RPV), canine distemper virus (CDV), sea dog pestivirus
(PDV), dolphin pestivirus (DDV), ox measles virus (MVKI) and people measles virus (MV) etc..The genome structure of PPRV is single
Stock minus strand, without segment RNA.
The diagnostic method of peste des petits ruminants mainly has Virus Isolation, antibody serum detection, antigen detection etc..Antigen
Detection method has agar gel Immune proliferation (AGID), counter immunoelectrophoresis (CIEP), indirect fluorescent antibody test (IFAT) etc.
Method.
Currently, effective ways there is no to treat peste des petits ruminants, vaccine inoculation, epidemic situation can only be taken to slaughter and periodically after occurring
The method of sero monitoring is controlled.Therefore, the serodiagnosis of the disease and the assessment of immune effect of vaccine seem with monitoring
It is particularly important.
World Organization for Animal Health recommends the PPR virus antibody detection method used mainly to have virus to neutralize examination
Test (VNT) and enzyme-linked immunosorbent assay (ELISA).Wherein, the testing result of virus neutralization tests method is accurate, and being that detection is small ruminates
The goldstandard of epizootic disease virus, but this method detection time is long and is unsuitable for detecting great amount of samples.In contrast, enzyme-linked immunosorbent assay
Specificity and sensibility is higher, detection time is shorter than virus neutralization tests and therefore the suitable a large amount of samples of detection are answered extensively
Detection for PPR virus antibody.
Enzyme-linked immunosorbent assay method for PPR virus antibody test mainly includes competitive enzyme-linked immune measurement
(c-ELISA), enzyme-linked immunosorbent assay (b-ELISA) and indirect enzyme-linked immunosorbent measurement (indirect ELISA) are blocked.C-ELISA and b-
The specificity and sensibility of ELISA is relatively high, is by clinical generally accepted PPR virus antibody detection method, example
C-ELISA method is used if the peste des petits ruminants diagnostic kit in the French laboratory BIRAD general in the world.But
C-ELISA and b-ELISA is both needed to using monoclonal antibody, this causes testing cost to greatly improve；Moreover, c-ELISA and b-
The cumbersome of ELISA, detection time long (although having been shortened relative to VNT), criterion are complicated.On the other hand, traditional
Indirect ELISA use from PPR virus intact proteins (such as H protein, N protein, F protein etc.) or recombination egg
White as envelope antigen to detect the antibody in serum, cost is lower than c-ELISA and b-ELISA；But since this method makes
It uses intact proteins as antigen, is easy to happen the wrong identification and non-specific identification of antibody, therefore, specificity and sensibility
All not as good as c-ELISA and b-ELISA.
Therefore, it is necessary to develop, testing cost is low, detection time is short, easy to operate, specific and high sensibility small anti-
Hay epizootic disease diagnostic kit.
Summary of the invention
In view of above-mentioned problems of the prior art, low, detection that the purpose of the present invention is to provide a kind of testing costs
Time short, easy to operate, specific and high sensibility peste des petits ruminants diagnostic kit, and can be used in preparing the reagent
The polypeptide or polypeptides in combination of box.
Indirect ELISA method based on antigen epitope polypeptide uses the antigen epitope polypeptide (single of 20 amino acid or so
Polypeptide usually only includes an epitope) it is used as envelope antigen.Inventor's discovery: the sensibility of this method is often relatively low, single
The sensibility of polypeptide does not exceed 50% generally；And the polypeptide that sensitivity is high, false positive rate are also high, i.e., it is specific low.If
The specificity and sensibility of detection can be improved simultaneously by certain mode, so that it may the shortcomings that overcoming traditional indirect ELISA,
Developing specificity and sensibility can match in excellence or beauty c-ELISA and b-ELISA, even higher peste des petits ruminants diagnostic kit.
Inventor has made intensive studies to solve above-mentioned technical problem, as a result, it has been found that following polypeptides in combination 1.When with this
It at least any one in following polypeptides in combination 1 when having response as index to the biological sample in object organisms source, can be with
92.2% sensibility and 93.5% specific diagnosis peste des petits ruminants, completely can be with c-ELISA method and the side b-ELISA
Method matches in excellence or beauty.
Therefore, the present invention includes:
1. a kind of peste des petits ruminants diagnostic kit comprising substrate, and under being individually secured in the substrate
State polypeptides in combination 1；
Polypeptides in combination 1
Sequence shown in SEQ ID NO:1 is the polypeptide of N50:SAEALFRLQAMAKILEDQEE,
Sequence shown in SEQ ID NO:2 is the polypeptide of N49:SSQNPREAQRSAEALFRLQA,
Sequence shown in SEQ ID NO:3 is the polypeptide of F54:LKPDLTGTSKSYVRSL,
Sequence shown in SEQ ID NO:4 is the polypeptide of F13:VATAAQITAGVALHQSLMNS,
Sequence shown in SEQ ID NO:5 is the polypeptide of N41:TGDERTVRGTGPRQAQVSFL, and
Sequence shown in SEQ ID NO:6 is the polypeptide of F52:CCCKGRCRNKEIPASKINPG.
2. being used for the biology by test object biological source according to peste des petits ruminants diagnostic kit described in item 1
Whether the antibody (IgG) that whether there is anti-PPR virus in sample diagnoses object organisms by PPR virus sense
Dye or peste des petits ruminants vaccine immunity.
3. according to peste des petits ruminants diagnostic kit described in item 2, wherein the object organisms small ruminant.
4. the peste des petits ruminants diagnostic kit according to item 2 or 3, wherein the object organisms are goat or sheep.
5. the peste des petits ruminants diagnostic kit according to any one of item 2~4, wherein the biological sample is complete
Blood, blood plasma or serum.
6. purposes of following polypeptides in combination 1 in preparation peste des petits ruminants diagnostic kit；
Polypeptides in combination 1
Sequence shown in SEQ ID NO:1 is the polypeptide of N50:SAEALFRLQAMAKILEDQEE,
Sequence shown in SEQ ID NO:2 is the polypeptide of N49:SSQNPREAQRSAEALFRLQA,
Sequence shown in SEQ ID NO:3 is the polypeptide of F54:LKPDLTGTSKSYVRSL,
Sequence shown in SEQ ID NO:4 is the polypeptide of F13:VATAAQITAGVALHQSLMNS,
Sequence shown in SEQ ID NO:5 is the polypeptide of N41:TGDERTVRGTGPRQAQVSFL, and
Sequence shown in SEQ ID NO:6 is the polypeptide of F52:CCCKGRCRNKEIPASKINPG.
7. according to purposes described in item 6, wherein the peste des petits ruminants diagnostic kit is used for through test object biology
It whether there is the antibody (IgG) of anti-PPR virus in the biological sample in source, whether diagnosis object organisms are ruminated by small
Epizootic disease virus infection or peste des petits ruminants vaccine immunity.
8. according to purposes described in item 7, wherein the object organisms small ruminant.
9. the purposes according to item 7 or 8, wherein the object organisms are goat or sheep.
10. the purposes according to any one of item 7~9, wherein the biological sample is whole blood, blood plasma or serum.
Aforementioned polypeptides and kit, which can be used for whether there is in the biological sample of test object biological source, resists small ruminate
The antibody (IgG) of epizootic disease virus.In general, the PPR virus antibody (IgG) in biological sample is due to object organisms quilt
PPR virus is infected or is generated by peste des petits ruminants vaccine immunity, and therefore, aforementioned polypeptides and kit can be used
In diagnosis object organisms whether by PPR virus infection or peste des petits ruminants vaccine immunity.
The specific embodiment of invention
Firstly, the present invention provides following polypeptides in combination 1:
Polypeptides in combination 1
Sequence shown in SEQ ID NO:1 is the polypeptide of N50:SAEALFRLQAMAKILEDQEE,
Sequence shown in SEQ ID NO:2 is the polypeptide of N49:SSQNPREAQRSAEALFRLQA,
Sequence shown in SEQ ID NO:3 is the polypeptide of F54:LKPDLTGTSKSYVRSL,
Sequence shown in SEQ ID NO:4 is the polypeptide of F13:VATAAQITAGVALHQSLMNS,
Sequence shown in SEQ ID NO:5 is the polypeptide of N41:TGDERTVRGTGPRQAQVSFL, and
Sequence shown in SEQ ID NO:6 is the polypeptide of F52:CCCKGRCRNKEIPASKINPG.
In the indirect ELISA method based on antigen epitope polypeptide, the sensibility for being usually no more than 50% of single polypeptide
It compares, is having response as index the biological sample in object organisms source using at least any one in the polypeptides in combination 1
In the case of, it can be with 92.2% sensibility and 93.5% specific diagnosis peste des petits ruminants.
In this specification, sensibility refers to: in the positive sample with the confirmation of " goldstandard " method, being measured as by other methods
The ratio of positive sample.Specificity refers to: in the negative sample with the confirmation of " goldstandard " method, being measured as feminine gender by other methods
The ratio of sample.For the detection of PPR virus antibody, " goldstandard " of the art is that virus neutralizes examination
Test (VNT).
Aforementioned polypeptides combination can be used as detection probe be used to prepare in the biological sample of test object biological source whether
There are the kits of the antibody of anti-PPR virus (IgG).In general, the PPR virus antibody in biological sample
It (IgG) is to be infected due to object organisms by PPR virus or generated by peste des petits ruminants vaccine immunity, on
It states polypeptides in combination and whether kit can be used for diagnosing object organisms by PPR virus infection or peste des petits ruminants epidemic disease
Seedling is immune.
Therefore, the present invention also provides a kind of peste des petits ruminants diagnostic kits comprising substrate, and be independently connected to
Aforementioned polypeptides combination 1 in the substrate.
In the present specification, substrate is normally solid, can be one, be also possible to it is multiple, but preferably one, i.e., entirely
Portion's polypeptide is independently connected in same substrate.In the present invention, substrate is not particularly limited, as long as solid or insoluble
Material support.The connection of polypeptide and substrate can be using well known to a person skilled in the art the connections of polypeptide and solid material
Method carries out.
In the present specification, the object organisms are preferably small ruminant, more preferably goat or sheep.
In the present specification, the biological sample can be whole blood, blood plasma or serum.
In the case where stating kit diagnosis peste des petits ruminants in use, as any in the polypeptides in combination 1 or one
When the above polypeptide has response to the biological sample in object organisms source, determine that the object organisms are infected by PPR virus
Or peste des petits ruminants vaccine immunity (i.e. positive)；Conversely, determining the object organisms not by PPR virus infection or small anti-
Hay epizootic disease vaccine immunity (i.e. negative).
In the present specification, " response " refers to: signal-to-noise ratio (SNR) is greater than or equal to 2, wherein signal-to-noise ratio=(polypeptide point letter
Number value-negative control point signal value)/negative control point signal value.
Embodiment
1. the preparation and confirmation of polypeptide
The polypeptide of NO:1~6 SEQ ID is synthesized by gill biochemistry (Shanghai) Co., Ltd., and is carried out by mass spectrum to it
Confirmation.Wherein,
The sequence of SEQ ID NO:1 is N50:SAEALFRLQAMAKILEDQEE；
The sequence of SEQ ID NO:2 is N49:SSQNPREAQRSAEALFRLQA；
The sequence of SEQ ID NO:3 is F54:LKPDLTGTSKSYVRSL；
The sequence of SEQ ID NO:4 is F13:VATAAQITAGVALHQSLMNS；
The sequence of SEQ ID NO:5 is N41:TGDERTVRGTGPRQAQVSFL；
The sequence of SEQ ID NO:6 is F52:CCCKGRCRNKEIPASKINPG.
2. the preparation of kit (polypeptide chip) 1
Kit 1
Polypeptide chip is the solution difference point sample by the polypeptide of NO:1~6 SEQ ID in solid support material --- " 0+X "
Be prepared on film (iPDMS film) (while one goat IgG of point sample is used as feminine gender as positive quality control point and a PB point
Quality Control point)." 0+X " film is that the initiator with olefin-terminal, surface initiated polymerization is added to dimethyl silicone polymer
In material, then in the three-dimensional structure in a manner of heat cross-linking (si-h bond bonding) fixed to dimethyl silicone polymer, obtain
A kind of material.Its manufacturing process can be found in published International patent WO2014/044184.
3. being detected with kit
Polypeptide chip is placed in room temperature 20min, visually observes chip surface, there will be the polypeptide chip of defect to eliminate, simultaneously
The correct direction for determining polypeptide chip, is sandwiched in tongue, and the number of chip is carried out according to the quantity of serum, then in qualified polypeptide
TBST (0.4M Tris-HCl, 2.74M NaCl, 2%Tween20, pH7.2 ± 0.2) is filled in chip, is placed at room temperature for 2-
4min checks for that in the dead of night, the polypeptide chip of leakage being discarded.The polypeptide chip of vacancy is filled up, operation is same as above.It is qualified to examine
Polypeptide chip be sandwiched on tongue, every 8 chips are one group, and with TBST rinse 2 times, watering can elution a, hole Kong Jinyi goes out, and are formed
Flowing, is finally gently patted on gauze, another one takes lid to dry, but it is noted that holding polypeptide chip surface wettability, standby
With.
Serum is diluted 50 times (+245 μ l serum dilutions of 5 μ l serum sample) in super-clean bench to number respectively, is shaken
It mixes, chip number is made to number consistent with each other, the loading on the chip of rinse with serum, 200 holes μ l/ avoid generating gas
Bubble, is added the 200 μ l of serum after diluting, room temperature, and shaking table 150rpm is incubated for 30min.
Liquid is abandoned, 4 times (method is same as above) is eluted, drying is added secondary antibody (rabbit-anti goat IgG), room temperature, and shaking table 150rpm is incubated
Educate 30min.
Liquid is abandoned, lid is abandoned, is eluted 4 times, drying, every 8 chips are one group, add luminescent solution (Thermo, the Prod# in 20 holes μ l/
37074) 2min, is exposed.Data are acquired using GenePix Pro6.0.
For every a serum, whether each polypeptide counted in the kit respectively has response (that is, signal-to-noise ratio
(SNR) it is more than or equal to 2), and determined.
For mentioned reagent box 1, when detect any one or one in polypeptide shown in NO:1~6 SEQ ID with
On when having response, be determined as the peste des petits ruminants positive；Conversely, being determined as feminine gender.
Wherein, signal-to-noise ratio=(polypeptide point signal value-negative control point signal value)/negative control point signal value.Polypeptide point
Signal value refers to the chemiluminescence intensity value for the polypeptide point that software is read, and negative control point signal value refers to the feminine gender that software is read
The chemiluminescence intensity value of control point.
For 755 parts of goats and sheep serum sample from China regions, it is respectively adopted and is prepared in above-mentioned steps 2
Kit (the step of pressing above-mentioned 3) and virus neutralization tests (VNT, by OIE (2010) " PPR virus neutralization test "
The method) it is detected, testing result is as follows.
Table 1
Sensibility=368/399*100%=92.2%
Specificity=333/356=93.5%
As known from the above, the sensibility and specificity of kit 1 (and is using VNT method validation, no 90% or more
It is to be verified using contrast agents cassette method), it is sufficient to the kit to match in excellence or beauty using c-ELISA method or b-ELISA method.
Sequence table
<110>China Veterinery Drug Inspection Office
Luoyang Zhong Ke Bioarray Solutions Ltd.
<120>peste des petits ruminants diagnostic kit
<130> PB00108
<141> 2018-04-24
<160> 6
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 1
Ser Ala Glu Ala Leu Phe Arg Leu Gln Ala Met Ala Lys Ile Leu Glu
1 5 10 15
Asp Gln Glu Glu
20
<210> 2
<211> 20
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 2
Ser Ser Gln Asn Pro Arg Glu Ala Gln Arg Ser Ala Glu Ala Leu Phe
1 5 10 15
Arg Leu Gln Ala
20
<210> 3
<211> 16
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 3
Leu Lys Pro Asp Leu Thr Gly Thr Ser Lys Ser Tyr Val Arg Ser Leu
1 5 10 15
<210> 4
<211> 20
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 4
Val Ala Thr Ala Ala Gln Ile Thr Ala Gly Val Ala Leu His Gln Ser
1 5 10 15
Leu Met Asn Ser
20
<210> 5
<211> 20
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 5
Thr Gly Asp Glu Arg Thr Val Arg Gly Thr Gly Pro Arg Gln Ala Gln
1 5 10 15
Val Ser Phe Leu
20
<210> 6
<211> 20
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 6
Cys Cys Cys Lys Gly Arg Cys Arg Asn Lys Glu Ile Pro Ala Ser Lys
1 5 10 15
Ile Asn Pro Gly
20
Claims (10)
1. a kind of polypeptides in combination 1, the polypeptide shown in NO:1~6 SEQ ID is formed, wherein
The sequence of SEQ ID NO:1 is N50:SAEALFRLQAMAKILEDQEE；
The sequence of SEQ ID NO:2 is N49:SSQNPREAQRSAEALFRLQA；
The sequence of SEQ ID NO:3 is F54:LKPDLTGTSKSYVRSL；
The sequence of SEQ ID NO:4 is F13:VATAAQITAGVALHQSLMNS；
The sequence of SEQ ID NO:5 is N41:TGDERTVRGTGPRQAQVSFL；
The sequence of SEQ ID NO:6 is F52:CCCKGRCRNKEIPASKINPG.
2. a kind of peste des petits ruminants diagnostic kit comprising:
Polypeptides in combination described in claim 1, wherein
It is used for the polypeptides in combination to be fixed on substrate, a kind of peste des petits ruminants diagnostic kit, institute is made jointly with substrate
Kit is stated for by resisting in the biological sample of test object biological source with the presence or absence of the IgG of anti-PPR virus
Body, whether diagnosis object organisms are by PPR virus infection or peste des petits ruminants vaccine immunity.
3. peste des petits ruminants diagnostic kit according to claim 2, wherein the object organisms small ruminant.
4. peste des petits ruminants diagnostic kit according to claim 2, wherein the object organisms are goat or sheep.
5. peste des petits ruminants diagnostic kit according to claim 2, wherein the biological sample be whole blood, blood plasma or
Serum.
6. purposes of following polypeptides in combination 1 in preparation peste des petits ruminants diagnostic kit；
Polypeptides in combination 1
Sequence shown in SEQ ID NO:1 is the polypeptide of N50:SAEALFRLQAMAKILEDQEE,
Sequence shown in SEQ ID NO:2 is the polypeptide of N49:SSQNPREAQRSAEALFRLQA,
Sequence shown in SEQ ID NO:3 is the polypeptide of F54:LKPDLTGTSKSYVRSL,
Sequence shown in SEQ ID NO:4 is the polypeptide of F13:VATAAQITAGVALHQSLMNS,
Sequence shown in SEQ ID NO:5 is the polypeptide of N41:TGDERTVRGTGPRQAQVSFL, and
Sequence shown in SEQ ID NO:6 is the polypeptide of F52:CCCKGRCRNKEIPASKINPG.
7. purposes according to claim 6, wherein the peste des petits ruminants diagnostic kit is used for raw by test object
It whether there is the IgG antibody of anti-PPR virus in the biological sample in object source, whether diagnosis object organisms are ruminated by small
Epizootic disease virus infection or peste des petits ruminants vaccine immunity.
8. purposes according to claim 7, wherein the object organisms small ruminant.
9. purposes according to claim 7, wherein the object organisms are goat or sheep.
10. purposes according to claim 7, wherein the biological sample is whole blood, blood plasma or serum.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113671182A (en) * | 2020-05-13 | 2021-11-19 | 洛阳中科生物芯片技术有限公司 | Antibody joint detection kit for gD and gE proteins of porcine pseudorabies virus, and preparation method and application thereof |
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| WO2014044184A1 (en) * | 2012-09-21 | 2014-03-27 | 苏州偲聚生物材料有限公司 | Protein for detecting type 1 diabetes mellitus and partial peptide thereof |
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| E. COUACY-HYMANN 等: "Interference in the vaccination of cattle against rinderpest virus by antibodies against peste des petits ruminants (PPR) virus", 《VACCINE》 * |
| 石晓妮 等: "小反刍兽疫病毒H和F蛋白多抗原表位的表达及其反应原性", 《中国兽医科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113671182A (en) * | 2020-05-13 | 2021-11-19 | 洛阳中科生物芯片技术有限公司 | Antibody joint detection kit for gD and gE proteins of porcine pseudorabies virus, and preparation method and application thereof |
| CN113671182B (en) * | 2020-05-13 | 2023-11-28 | 洛阳中科生物芯片技术有限公司 | Antibody joint inspection kit for porcine pseudorabies virus gD and gE proteins and preparation method and application thereof |
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| CN110498845B (en) | 2021-03-09 |
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