CN1104860A - Preparing method for difficult digestion dextrin - Google Patents

Abstract

The present invention relates to a kind of method for preparing functional food base material-hardly digestible dextrin, which takes starch as base material, hydrochloric acid as catalyst, the product is obtained through the following process, high temp. processing to obtain scorched dextrin, using temp. resistant amylase and fungus alpha-amylase to undergo enzymic processing, using active carbon to decolor, refining by ion exchange resin and spray-drying. The glucose content of the product is little. The difficult spray-drying and complicated process are solved. The colour tone of the product is low. The applying range is wide, compared with the background technologies the cast is lowered obviously.

Description

Preparing method for difficult digestion dextrin

The present invention relates to a kind of preparation method of functional food ingredient, is the preparation method that the 6th kind of nutrient after sugar, protein, fat, vitamin, mineral matter promptly is widely used in a kind of food fiber-difficult digestion dextrin of food and medicine industry specifically.

The production method of difficult digestion dextrin in being the European patent of EP0535627A1, the patent No. is disclosed, difficult digestion dextrin is a kind of as water-soluble food fiber, be to use starch under acid catalysis, make high-temperature process, get pyrodextrin, again through enzyme handle, refining, spray-drying and obtain difficult digestion dextrin then.In developed countries such as Japan, the output of difficult digestion dextrin occupies second of various food fiber output, is only second to polymerization glucose.The weak point of this method is, after glucoamylase is handled, be the compound sugar of solubility just with unreacted starch degradation, and it is little to the content influence of difficult digestion dextrin, products obtained therefrom is similar to syrup, it is many to contain glucose, therefore spray drying technology is required height and complex process, so that yield is low, cost is high.With regard to domestic, do not see any research paper and relevant report as yet about difficult digestion dextrin, more do not see this homemade goods.

The objective of the invention is to avoid the defective of above-mentioned background technology, select for use certain not change the content of difficult digestion dextrin in the product, only change the proportion of composing of all the other compound sugar, reduce the enzyme preparation of glucose content, provide a kind of yield height, cost low, with short production cycle, the product colour is shallow and the scope of application is wideer, the preparation method of spray-dired difficult digestion dextrin easy to implement.

Purpose of the present invention can reach by following measure:

With starch is base stock, comprise that high-temperature process, enzyme are handled and the preparation method of a kind of difficult digestion dextrin of subtractive process, it is characterized in that the present invention adopts heatproof amylase and fungal alpha-amylase to handle pyrodextrin, make difficult digestion dextrin, it comprises following processing step and process conditions thereof:

One, the preparation of pyrodextrin:

With the commodity starch is base stock, be that 1～3% hydrochloric acid is made catalyst by 3～10% weight percent concentration of over dry amylometer earlier with the way adding of spraying, after stirring, preheating is 1～4 hour under 100～120 ℃ of temperature conditions, the water content of starch is reduced to below 5%, be warming up to 140～200 ℃ then, baked 0.5～4 hour, reaction generates pyrodextrin;

Two, enzyme is handled:

The pyrodextrin that step 1 is made adds water, make pyrodextrin content and be 30～50% emulsion by weight percentage, transferring pH value is 6.0～6.5, add 0.05～0.6% heatproof amylase by weight percentage, 100～105 ℃ of complete gelatinizations down, 90～95 ℃ of liquefaction down, then, be warming up to 110～120 ℃ of enzymes that go out, be cooled to 50～55 ℃, transferring pH value is 5.0～5.5, adds 0.05～0.3% fungal alpha-amylase by weight percentage again, be incubated 50～55 ℃, react and be warming up to 80 ℃ of enzymes that go out after 18～48 hours;

Three, post processing:

Use activated carbon decolorizing, filter, ion exchange resin is refining, last spray-drying.

Table 1 is the product constituent and the difficult digestion dextrin content table of comparisons of embodiment 1,2 and comparative examples 1,2.

Table 2 is the product colour table of comparisons of embodiment 1,2 and comparative examples 1,2.

The present invention compares with background technology has following advantage:

1, by table 1 explanation, it is few that the difficult digestion dextrin that adopts the present invention to make contains glucose, it is many to contain the above compound sugar of maltose, trisaccharide, tetrose and tetrose, just can produce with general spray drying device, has overcome the spray-drying difficulty that the glucose content height brings, the shortcoming of complex process.Owing to solved this difficult point of spray-drying, just can impel the production of difficult digestion dextrin to promote as early as possible in China, the while also is the improvement that promotes in the world the difficult digestion dextrin production technology.

2, by table 2 explanation, the difficult digestion dextrin colour that adopts the present invention to make is shallow, and color is that white is to light yellow, its solution is water white transparency or faint yellow transparent, as the base-material of functional food, the scope of application is widened, and can be used as colourless or light beverage, the additive of food.

3, this law is compared integrated cost with background technology and is obviously descended, and this mainly is because the fungal alpha-amylase processing time is short, shortens the production cycle, has improved production efficiency; The fungal alpha-amylase consumption is few, and production cost reduces; Because the not sticking wall of spray-drying also just makes product easily reclaim, improved yield, thereby also reduced production cost again.

The present invention is described in further detail below in conjunction with embodiment and comparative examples:

Embodiment 1:

Take by weighing 100 kilograms of tapiocas, earlier adding the weight percent concentration with the way of spraying is that 1% hydrochloric acid is made catalyst for 8 liters, after stirring, preheating is 2 hours under 120 ℃ of temperature conditions, and the water content of starch is reduced to below 5%, is warming up to 180 ℃ then, baked 1 hour, reaction generates pyrodextrin.Add 200 liters water again and stir into even emulsion, with weight percent concentration 20% NaOH, transfer pH value to 6.2, add 0.15% heatproof amylase by weight percentage by the production of Wuxi City enzyme preparation factory, 105 ℃ of following gelatinizations 5 minutes, liquefied 1 hour down at 95 ℃, be warming up to 120 ℃ of enzymes that go out then, be cooled to 55 ℃, transferring pH value is 5.3, add by weight percentage 0.1% the fungal alpha-amylase of producing by Denmark NoVo company, be incubated 55 ℃, react and be warming up to 80 ℃ of enzymes that go out after 24 hours.Use the powder activity carbon decoloring at last, filter, ion exchange resin is refining, and spray-drying gets the product difficult digestion dextrin.The content of difficult digestion dextrin and the constituent of product are measured with the HPLC method, the results are shown in Table 1.

Embodiment 2:

Take by weighing 100 kilograms of cornstarch, earlier adding the weight percent concentration with the way of spraying is that 3% hydrochloric acid is made catalyst for 2.5 liters, after stirring, preheating is 2 hours under 110 ℃ of temperature conditions, the water content of starch is reduced to below 5%, be warming up to 160 ℃ then, baked 2 hours, reaction generates pyrodextrin, add 200 liters water again and stir into even emulsion, with weight percent concentration is 20% NaOH, transfers pH value to 6.3, adds by weight percentage 0.15% the heatproof amylase of being produced by Denmark NoVo company, 105 ℃ of following gelatinizations 5 minutes, liquefied 1 hour down at 95 ℃, be warming up to 120 ℃ of enzymes that go out then, be cooled to 55 ℃, transferring pH value is 5.5, add by weight percentage 0.2% the fungal alpha-amylase of producing by Denmark NoVo company, be incubated 55 ℃, react and be warming up to 80 ℃ of enzymes that go out after 24 hours.Use the powder activity carbon decoloring at last, filter, ion exchange resin is refining, mist drying, product difficult digestion dextrin.The content of difficult digestion dextrin and the constituent of product are measured with the HPLC method, the results are shown in Table 1.

Comparative examples 1:

Take by weighing 100 kilograms of tapiocas, dezymotize and handle outside some process conditions and the enzyme preparation, other process conditions embodiment 1, the enzyme processing procedure is as follows:

With pyrodextrin content is 30～50% emulsion by weight percentage, transferring pH value is 6.0, adding by weight percentage, 0.6% heatproof amylase liquefies down at 95 ℃, transferring pH value is 4.5, add by weight percentage 0.4% the glucoamylase of producing by Denmark NoVo company, react and be warming up to 80 ℃ of enzymes that go out in 64 hours afterwards; Content and constituent with HPLC method mensuration difficult digestion dextrin the results are shown in Table 1.

Comparative examples 2:

Take by weighing 100 kilograms of cornstarch, processing method is with comparative examples 1, and content and constituent with HPLC method mensuration difficult digestion dextrin the results are shown in Table 1.

Table 1:

	Product is formed (%)				The smart content % in indigestibility lake
						Glucose	Disaccharides	Trisaccharide	Tetrose and more than the tetrose
	Embodiment 1	5.09	23.81	13.68						57.42	50.58
Embodiment 2					3.45	23.19	7.85	65.51	51.68
	Reference examples 1	43.95	1.67	1.53						52.85	50.58
Reference examples 2					45.87	1.36	0	52.77	51.68

Table 2:

Sample	Embodiment 1	Embodiment 2	Reference examples 1	Reference examples 2
					Absorbance	0.308	0.205	0.523	0.736

Claims (1)

1, with starch is base stock, comprise that high-temperature process, enzyme are handled and the preparation method of a kind of difficult digestion dextrin of subtractive process, it is characterized in that, the present invention adopts heatproof amylase and fungal alpha-amylase to handle pyrodextrin, make difficult digestion dextrin, it comprises following processing step and process conditions thereof:

One, the preparation of pyrodextrin:

With the commodity starch is base stock, be that 1～3% hydrochloric acid is made catalyst by 3～10% weight percent concentration of over dry amylometer earlier with the way adding of spraying, after stirring, preheating is 1～4 hour under 100～120 ℃ of temperature conditions, the water content of starch is reduced to below 5%, be warming up to 140～200 ℃ then, baked 0.5～4 hour, reaction generates pyrodextrin;

Two, enzyme is handled:

The pyrodextrin that step 1 is made adds water, make pyrodextrin content and be 30～50% emulsion by weight percentage, transferring pH value is 6.0～6.5, add by weight percentage 0.05～0.6% heatproof amylase and pound 100～105 ℃ of complete gelatinizations down, 90～95 ℃ of liquefaction down, then, be warming up to 110～120 ℃ of enzymes that go out, be cooled to 50～55 ℃, transferring pH value is 5.0～5.5, add 0.05～0.3% fungal alpha-amylase by weight percentage again, be incubated 50～55 ℃, react and be warming up to 80 ℃ of enzymes that go out after 18～48 hours;

Three, post processing:

Use activated carbon decolorizing, filter, ion exchange resin is refining, last spray-drying.