CN110483605A - The preparation and its application of one plant growth regulators - Google Patents
The preparation and its application of one plant growth regulators Download PDFInfo
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- CN110483605A CN110483605A CN201910756457.8A CN201910756457A CN110483605A CN 110483605 A CN110483605 A CN 110483605A CN 201910756457 A CN201910756457 A CN 201910756457A CN 110483605 A CN110483605 A CN 110483605A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
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Abstract
The present invention relates to the preparation of a plant growth regulators and its application, the plant growth regulator is 14- hydroxyl brassinosteroids, and feature is more easy to get for raw material, and cheap, and preparation method is simple and feasible, and application method is simple.The preparation and its application of 14- hydroxyl brassinosteroids described herein, have the function of promoting plant growth, promote fruits and vegetables to bear fruit, vernalization, improving quality and degeneration-resistant aspect, and class regulator, classes of herbicides, abscisic acid and biochemical class regulator can be inhibited to compound with cell classification element, gibberellic acid class, fungicide, plant, significantly improve drug effect and stability, increase the utilization rate of preparation, is suitble to industrialized production.
Description
Technical field
The present invention relates to chemical industry and agriculture field more particularly to a plant growth regulators 14- hydroxyl brassinosteroids
Preparation and its application.
Background technique
Brassin is the plant growth regulator of a kind of wide spectrum, high activity.Its activity is mainly shown as promotion plant growth,
Setting percentage is improved, yield, improving quality, degeneration-resistant etc. are increased.It is the effective component and other structures of brassin lactones, functionally similar
Brassin substance, the 6th class plant being known as after auxin, gibberellin, the basic element of cell division, abscisic acid and ethephon (CEPHA),2-(chloroethyl) phosphonic acid
Hormone.Since the physiological activity of substance of this kind is high, the effect for adjusting growth and development is unique, and extremely small dosage can show
Good regulating effect out.It is as the 6th class plant growth regulator, compared with preceding 5 class plant growth regulator, volume increase,
Degeneration-resistant, solution phytotoxicity, drop agriculture is residual etc. has a clear superiority.Its mode of action is chiefly to facilitate cell growth and division, regulation
Blade shape；Change cell membrane potential and enzymatic activity, enhances photosynthesis；The biosynthesis for promoting DNA, RNA and protein, mentions
The tolerance etc. of high plant pair environment-stress.
The discovery of brassin lactones is derived from 1970, and American scholar J.W.Mitchell etc. extracts one from rape pollen
Kind has the very active substance of Johnson & Johnson's reason, and elongation and cell division to axis have strong facilitation, name as rape
Element, also known as brassin (Brassins).American scholar Grove in 1979 has harvested the oil of 227kg using the method for honeybee acquisition
Cauliflower powder has obtained the rape element of 4mg after separating-purifying, has been analyzed by instrument and determine that its chemical structure belongs to sterol lactone, therefore
It is named as brassinosteroid and is also referred to as brassin lactones (Brassinolide, abbreviation BL).It is brilliant through mass spectrum, infrared and X-ray
Body analyzes the configurational formula of brassinosteroid, and chemical name is 2 α, 3 α, 22 α, -24 α methyl-B- homotype of 23 α -4 hydroxyl -
- 5 α of 7- oxygen--6 ketone of cholestene.
Hitherto it is found that about 70 kinds of brassinolide class compounds, are collectively referred to as brassinosteroid substance
(brassinosteroid, BR).BR is widely present in plant, and since their function is similar to BL, BR becomes oil
In the generation of dish element sterol substance, claims, and BL is the strongest a kind of molecule of activity in brassinosteroid member.1998 the 13rd
It by official confirmation is the 6th class plant hormone in International Plant growth substance annual meeting.
Influence of the brassin lactones to plant growth and development includes: that seed can be promoted to sprout；And promote cell in cauline leaf
Extension and cell division；Not only promote but also inhibit the growth of root；Promote the differentiation of xylem in conduit development；It is also that pollen tube is raw
Action component necessary to long.Crop it is degeneration-resistant, prevention disease, repair fruit tree degeneration function, reduce plant content of beary metal, mention
The research for rising crop growth function etc. on alkaline land soil is also in progress.Currently, brassin lactones preparation is still main
Will based on single dose, but its it is mixed with other preparations or it is mixed with other plant growth regulator also will be following developing direction
One of.
More important rape chlorins compound (BR) includes 14- hydroxyl brassinosteroids, 28- high brassin lactones, 28- table
High brassin lactones, 24- table brassin lactones, 22,23,24- table brassin lactones etc..14- hydroxyl brassinosteroids are rapes
Important a member in plain family, 14- hydroxyl brassinosteroids are mainly using rape petal pollen and beeswax as raw material, using enzyme process water
Solution abstraction technique extracts, separates and purifies the nature brown cotton being prepared based on 14- hydroxyl brassinosteroids, can be
Stablize under acid, neutrallty condition, toxicity level is less toxic or micro- poison, low to the environmental organisms toxicity such as aquatile.But due to it
Content is extremely low in natural plants, extracts separation especially purifying and needs to consume a large amount of raw materials and reagents, higher cost, and produces
It measures limited.
Summary of the invention
In view of the drawbacks of the prior art, the invention proposes a plant growth regulators 14- hydroxyl brassinosteroids
Preparation and its application, feature are more easy to get for raw material, and cheap, and preparation method is simple and feasible, and application method is simple, and
It is different depending on the application, it can be compounded with Multiple components, expand its application field, efficiency is higher, and cost is relatively low, therefore has extremely wide
General development and application prospect.
Preferably, the plant growth regulator 14- hydroxyl brassinosteroids are using moulting hormone and its derivative as raw material
By adding hydrogen or reduction method to be prepared, used catalyst is metallic catalyst, including lithium amide, tetrahydrochysene lithium aluminium, sodium borohydride/
Cuprous iodide or Pd/C, Pt/C, Pd/ (OH)2, raney nickel, reaction temperature be -78 DEG C~30 DEG C.
Preferably, the mass percentage content of the plant growth regulator in the formulation are as follows: 14- hydroxyl brassinosteroids
0.001~1%.
Preferably, the dosage form of the preparation is liquid preparation, soluble powder, milk powder agent or compound formulation, wherein 14- hydroxyl rue
Tongue fur element sterol is 0.005%~1%.
Plant growth regulator provided by the present invention and preparation provided by the present invention are promoting plant growth, are promoting
Fruits and vegetables germinate, bear fruit, improving the application of quality and degeneration-resistant aspect.
The fruits and vegetables include fruits and vegetables；The fruit be any one be selected from grape, citrus, lichee, apple, pears,
Longan, loquat, plum, strawberry, banana, cherry, mango, Kiwi berry, peach or apricot；Watermelon, muskmelon, "Hami" melon etc. are melon, described
Vegetables are that any one is selected from tomato, cucumber, sponge gourd, long bean, eggplant, balsam pear, radish, carrot, garden pea, pea, blueness
Green pepper or capsicum, green onion, garlic, ginger, leek, beet, potato, pachyrhizus, taro and leafy vegetable.
The application further includes in tealeaves, tobacco, cotton, soybean, rape, peanut, mung bean, rice, wheat, corn, flower
Application in terms of grass, Chinese medicine, lawn.
Beneficial effect
The preparation and its application of 14- hydroxyl brassinosteroids proposed by the present invention, using 20- hydroxyl ecdysterone and its spread out
Biology is reaction substrate, and raw material is cheap and easy to get, and preparation method is simple and feasible, and application method is simple.
Plant growth regulator provided by the present invention and provided preparation can be separately as plant growth regulator
It uses, or compounds with fertilizer, fungicide, Insecticides (tech) & Herbicides (tech) etc. and used as additive, fertilizer efficiency and agricultural products can be improved
Effect.
Detailed description of the invention
1, Fig. 1 is the HPLC map of the 14- hydroxyl brassinosteroids of embodiment 3；
2, Fig. 2 is the LCMS spectrogram of the 14- hydroxyl brassinosteroids of embodiment 3
3, Fig. 3 is the MS spectrogram of the 14- hydroxyl brassinosteroids of embodiment 3；
4, Fig. 4 is the 14- hydroxyl brassinosteroids of embodiment 31HNMR spectrogram；
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Specific embodiment illustrates synthetic method with following embodiments and is applied to synthesis and agriculture field.
The preparation of embodiment 1:14- hydroxyl brassinosteroids
Reducing agent: lithium amide, tetrahydrochysene lithium aluminium, sodium borohydride/cuprous iodide etc.；
Reaction temperature: -78 DEG C -10 DEG C；
Solvent: THF；
Synthesis step: dry THF being added in dry there-necked flask, stirs lower slow cooling to 0 DEG C, at low temperature
It is slowly added lithium aluminium hydride solid powder 2.5g, the 30mlTHF solution of moulting hormone 10g is slowly added drop-wise to reaction after adding
At 0 DEG C, the reaction was continued in system, after adding, and the water quenching reaction of 20ml, reaction system acetic acid second is added after reaction in 2h
Ester extracts, and obtains white product, yield 85% with alcohol crystal after organic phase precipitation.
The synthesis of embodiment 2:14- hydroxyl brassinosteroids
Catalyst: the metallic catalysts such as Pd/C, Pt/C, Pd/ (OH) 2, Raney's nickel；
Reaction temperature: 24-80 DEG C；
Reaction pressure, 0-3Mpa；
Solvent: methanol, ethyl alcohol.
Implementation steps:
Weigh 10.0 grams of 20- hydroxyl ecdysterones, the wet Pd/C of 0.1g 10%, the sodium nitrite of 20ml 25% is water-soluble
Liquid, the ethyl alcohol of reaction dissolvent 40ml react 2h under hydrogen normal pressure.Filtering filters off Pd/C after fully reacting, and filtrate is evaporated, and uses
Ethyl alcohol dissolves remaining solid, and ethanol solution condensing crystallizing obtains product 8.9g, yield 90%.
The property and structure determination of embodiment 3:14- hydroxyl brassinosteroids
3.1 essential information
Chinese: 14- hydroxyl brassinosteroids；
English name:
(2S,3R,5S,10R,13R,14R)-2,3,4-trihydroxy-10,13dimethyl-17-((2R,3R)-2,
3,6,-trihydroxhylheptan-2-yl)-tettadecahydro-9H-cyclopenta[a]phenantren-6
(14H)-one
Molecular formula: C27H46O7
Molecular weight: 482.65
Chemical structure:
3.2 physical property measurement
Appearance: white powder；
Dissolubility: it is dissolved in methanol, ethyl alcohol, the organic solvents such as chloroform；
Acid-base property: neutral；
Stability: acid, stable under alkaline conditions exists.
3.3 spectroscopic assay
Using instrumental method test sample (embodiment 2), attached drawing 1, LC-MS spectrum are seen from gained HPLC-UV detection
Figure attached drawing 2, qualitative mass spectrogram attached drawing 3 and nuclear magnetic resonance spectroscopy attached drawing 4, confirmation gained are target product 14- hydroxyl brassin steroid
Alcohol.
The preparation of embodiment 4:0.01%14- hydroxyl brassinosteroids aqua
4.1 raw material
14- hydroxyl brassinosteroids 100mg, to lauryl sodium sulfate 2g, ethyl alcohol 50g, deionized water.
4.2 preparation step
(1) 15% ethanol solution of lauryl sodium sulfate will be dissolved, solution is colorless and transparent.
(2) the 14- hydroxyl brassinosteroids for synthesizing embodiment 1 are added in solution, supplement deionized water to 1000ml.
(3) pH=7.0 of solution is measured.
4.3 application method
(1) it is diluted to normal concentration before use, adjusts the pH=6.0-7.0 of solution.
(2) it is sprayed on plant leaf surface or root as required.
(3) it can be mixed with fertilizer or inhibit class regulator, weeding with cell classification element class, gibberellic acid class, fungicide, plant
It is applied after agent class, abscisic acid and biochemical class regulator compounding.
Embodiment 5:14- hydroxyl brassinosteroids and gibberellic acid compound preparation
5.1 raw material
14- hydroxyl brassinosteroids 0.1%, gibberellic acid 2.9%, ethyl alcohol, deionized water.
5.2 preparation step
(1) 5~10 times of ethyl alcohol of gibberellic acid is dissolved, solution is colorless and transparent.
(2) it by 14- hydroxyl brassinosteroids, is added in solution, supplement deionized water to 1000ml.
(3) pH=6.5~7.0 of solution are adjusted.
5.3 application method
(1) it is diluted to normal concentration before use, adjusts pH=6.5~7.0 of solution.
(2) it is sprayed on plant leaf surface or root as required.
Embodiment 6:14- hydroxyl brassinosteroids and cycocel compound preparation
6.1 raw material
14- hydroxyl brassinosteroids 0.1%, cycocel 2.9%, methanol, deionized water.
6.2 preparation step
(1) cycocel 50ml methanol is dissolved, solution is colorless and transparent.
(2) 14- hydroxyl brassinosteroids are added in solution, supplement deionized water to 500ml.
(3) pH=6.5~7.5 of solution are adjusted.
6.3 application method
(1) it is diluted to normal concentration before use, adjusts pH=6.5~7.0 of solution.
(2) it is sprayed on plant leaf surface or root as required.
Embodiment 7: determination of activity experiment
7.1 materials and method
(1) experimental material
For studying object: mung bean
Reagent agent: A:24- table brassin lactones, B: rape element sterone, C:14- hydroxyl brassinosteroids.
(2) experimental method
Mung bean is immersed in distilled water 30 minutes at 30 DEG C, is then placed on waterlogged filter paper, is put
It sets 4 hours in the dark.The seed for publishing bud or growth later, which places it on filter paper, to be immersed in different experimental pharmacies,
Test compares various concentration respectively.Positive control is done with 24- table brassin lactones and rape element sterone, does blank control with water.
7.2 experimental phenomenas and interpretation of result
Observation and measurement mung bean 3 days growth change situations under 27 DEG C of illumination, including mung bean hypocotyl is long, root long and fresh
Weight, the result is shown in table 7-1, table 7-2, table 7-3.
Influence (mm) of three kinds of experimental pharmacies to mung bean hypocotyl length under table 7-1 various concentration
| Concentration (ppm) | A:24- table brassin lactones | B: rape element sterone | C:14- hydroxyl brassinosteroids | CK |
| 5 | 13.3 | 25.3 | 36.3 | 11 |
| 0.5 | 19.8 | 35.2 | 34.5 | 11 |
| 0.05 | 37.5 | 50.8 | 32.9 | 11 |
| 0.005 | 31.8 | 39.8 | 38.0 | 11 |
| 0.0005 | 38.5 | 32.1 | 44.5 | 11 |
Influence (mm) of three kinds of experimental pharmacies to mung bean root long under 7-2, various concentration
| Concentration (ppm) | A:24- table brassin lactones | B: rape element sterone | C:14- hydroxyl brassinosteroids | CK |
| 5 | 11.0 | 12.4 | 65.4 | 54.3 |
| 0.5 | 12.2 | 11.8 | 57.6 | 54.3 |
| 0.05 | 13.6 | 18.4 | 65.7 | 54.3 |
| 0.005 | 22.3 | 37.8 | 63.2 | 54.3 |
| 0.0005 | 57.2 | 47.9 | 59.5 | 54.3 |
Influence (g) of three kinds of experimental pharmacies to mung bean fresh weight under table 7-3, various concentration
| Concentration (ppm) | A:24- table brassin lactones | B: rape element sterone | C:14- hydroxyl brassinosteroids | CK |
| 5 | 9.6 | 9.7 | 6.9 | 5.8 |
| 0.5 | 8.0 | 8.7 | 6.8 | 5.8 |
| 0.05 | 6.6 | 6.4 | 6.4 | 5.8 |
| 0.005 | 6.3 | 6.9 | 6.5 | 5.8 |
| 0.0005 | 6.1 | 6.8 | 8.0 | 5.8 |
From the analysis of experimental results of table 7-1 it is found that 14- hydroxyl brassinosteroids and 24- table brassin lactones or rape element
Sterone can preferably promote to grow under 0.0005ppm concentration compared to it.
It also can be seen that by table 7-2 data, 14- hydroxyl brassinosteroids can promote mung bean root growth and 24- table brassin
Or rape element sterone is obvious compared in high concentration synergy, while the plant growing way of 14- hydroxyl brassinosteroids processing is better than rue
Tongue fur element and rape element sterone.
Meanwhile 14- hydroxyl brassinosteroids can promote the growth of mung bean to table 7-3 out as the result is shown, fresh weight increases bright
It is aobvious；Its fresh weight increases by 37.9% under 0.0005ppm molar concentration.7.3 conclusion
It is drawn a conclusion by analysis of experimental results are as follows: the plant growing way of 14- hydroxyl brassinosteroids processing is better than control group, and
It is better than 24- table brassin lactones and rape element sterone, effect is obviously promoted to growth of mung beans.
The contrast and application test of embodiment 8:14- hydroxyl brassinosteroids on the cotton
8.1 materials and methods
8.1.1 test material
For studying object: cotton
Reagent agent: 14- hydroxyl brassinosteroids, 24- table brassin lactones and 28- high brassin lactones.
Test site: Zhongmou County port owned by the government Experimental Base.
8.1.2 test method
(1) laboratory test
Uniform cotton seeds are selected, 15min are sterilized with 15% hydrogenperoxide steam generator, with distilled water flushing 3 times
After dry, then respectively with 14- hydroxyl brassin, 24- table brassin lactones and 28- high brassin lactones seed soaking for 24 hours, set respectively
0.005ppm, 0.01ppm, 0.02ppm totally 3 concentration, if clear water processing is blank control.24 hours cotton kinds will be impregnated
Son is successively evenly spaced on the filter paper moistened in corresponding germination box, and 30 seeds of every processing are repeated 3 times；
It respectively in the germinative number of investigation seed daily, is not being investigated until continuous two days germinative numbers are identical, statistics seed hair
Bud rate；The uniform 10 plants of seedling of growing way are chosen in each processing in 10th day, measure the plant height and root long of seedling.
(2) field trial
Field trial uses cauline leaf spraying treatment, in 14- hydroxyl brassin, 24- table brassin lactones and 28- high brassin
Ester sets 3 concentration of 0.01ppm, 0.02ppm and 0.04ppm respectively, if clear water compares CK, each plot area is 20m2, each
Processing is repeated 3 times, and cell uses RANDOMIZED BLOCK DESIGN, and field management is consistent, is respectively administered 1 time in cotton in seedling stage, cotton buds and bolls phase.
Before cotton topping, cotton plant height is measured；In cotton harvesting, each cell randomly selects 20 plants, investigates cotton boll
Several and Single boll weight.
8.2 interpretation of result
8.2.114- influence of the hydroxyl brassinosteroids seed soaking to cotton
By table 8-1 it is found that 14- hydroxyl brassinosteroids, 24- table and 28- high brassin lactones seed soaking can be obvious
Improve the germination percentage of cotton seeds, the plant height and root long of seedling.24- table brassin lactones send out cotton seeds in set concentration
Bud rate, shoot growth and root growth show facilitation, and compared with the control, growth rate is respectively (9.09%, 12.04%
With 9.50%), (5.20%, 8.48% and 4.99%) and (6.50%, 5.19% and 2.87%), wherein in 0.005ppm timeliness
Fruit is preferable, compared with the control significant difference；28- high brassin lactones are in set concentration, to Cotton Seed rate, young shoot and root
System growth show facilitation, compared with the control, growth rate be respectively (5.20%, 15.29% and 12.79%),
(9.09%, 13.34% and 17.24%) and (6.50%, 8.64% and 11.50%), and to young shoot and root growth, and compares
Compared to significant difference；14- hydroxyl brassin is in set concentration, to Cotton Seed rate, shoot growth and the equal table of root growth
Effect is now remarkably promoted, growth rate is respectively (10.39%, 20.40% and 20.29%), (9.09%, 17.27% and
24.81%) and (9.09%, 13.42% and 23.34%), and effect is better than 24- table and 28- high brassin lactones.
Influence of the table 8-1 14- hydroxyl brassinosteroids seed soaking to cotton
8.2.2 14- hydroxyl brassinosteroids foliar spray handles the influence to cotton
By table 8-2 it is found that 14- hydroxyl brassinosteroids, 24- table and 28- high brassin lactones spray cotton plants
Significantly improve plant height, single plant cotton boll number and the single cotton boll weight of cotton.24- table brassin lactones are in set concentration, to cotton strain
High, single plant cotton boll number and single cotton boll weight show facilitation, but difference is not significant compared with the control, and growth rate is respectively
(2.51%, 2.97% and 1.57%), (7.68%, 12.58% and 4.25%) and (4.12%, 10.22% and 0.99%；28-
High brassin lactones show facilitation in set concentration, to cotton plant height, single plant cotton boll number and single cotton boll weight, and compare
Compare, growth rate be respectively (9.42%, 14.51% and 2.86%), (11.59%, 16.16% and 6.47%) and (4.12%,
10.65% and 6.82%)；14- hydroxyl brassin weighs cotton plant height, single plant cotton boll number and single cotton boll equal in set concentration
Show facilitation, compared with the control, growth rate be respectively (14.61%, 19.84% and 8.39%), (17.90%,
10.02%) and (15.46%, 19.91% and 7.17%) 26.94% and, wherein in 0.02ppm to cotton growth and yield
Facilitation effect is best, compared with the control significant difference, and effect is better than 24- table and 28- high brassin lactones.
Influence of the table 8-2 14- hydroxyl brassinosteroids foliar spray processing to cotton
8.3 conclusion
14- hydroxyl brassinosteroids are a kind of extremely strong novel sterol substances of activity, have efficient, low toxicity, activity strong
Effect, be a kind of higher plant growth regulator of activity.It is tested by this, 14 hydroxyl brassinosteroids are in set concentration
Seed soaking shows the effect of remarkably promoting to Cotton Seed rate, shoot growth and root growth；Plant is sprayed in set concentration,
Facilitation is showed to cotton plant height, single plant cotton boll number and single cotton boll weight, and with concentration 14- hydroxyl brassinosteroids, 24-
Table and the comparison of 28- high brassin lactones, 14- hydroxyl brassinosteroids effect are better than 24- table and 28- high brassin lactones.According to
It this time tests, seed soaking recommended density is the 14- hydroxyl brassinosteroids of 0.005ppm~0.01ppm, cauline leaf spraying treatment
Recommended density is the 14- hydroxyl brassinosteroids of 0.01~0.04mg/kg.
Embodiment 9:14- hydroxyl brassinosteroids and application effect 9.1 material and side of the cycocel compounding agent on wheat
Method
9.1.1 test material
For studying object: wheat；
Reagent agent: compounding agent (embodiment 6), 40% cycocel；
Test site: Zhongmou County's port owned by the government village Experimental Base.
9.1.2 test method
Test is arranged using random district's groups, and every 10 ㎡ of cell is repeated 3 times.Selection is the same as batch sowing, identical management, growth potential
Consistent wheat is as test material.
Field trial use cauline leaf spraying treatment, 14- hydroxyl brassinosteroids and cycocel compounding agent setting 50mg/L,
2 concentration of 100mg/L and 200mg/L are arranged in 3 concentration of 100mg/L and 200mg/L, 40% cycocel, if clear water compares CK,
It is respectively handled 1 time in 3 leaf phase of wheat and wheat during jointing stage, other management are the same as crop field routine.
9..1.3 investigation statistics
Florescence measures wheat plant lodging resistance index of correlation, is preferably located with screening to wheat lodging resistance effect
Reason.With the length between ruler and tape measure plant height, wheat base portion Section 1 between Section 2.
Harvest time, investigation wheat spike length, single-strain tassel number, grain number per spike and mass of 1000 kernel.
5 point samplings are all made of when investigation, 5 points of every cell samplings, look into 50 plants altogether by 10 plants every.Test data DPS
(trial edition) and Excel software carry out correlation statistical analysis.
2 interpretations of result
9.2.1 influence of each processing to Plant Height in Wheat and panel length
By table 9-1 it is found that 14- hydroxyl brassinosteroids and cycocel compounding agent are in 50mg/L, 100mg/L and 200mg/L
When, inhibiting effect is showed to Plant Height in Wheat, first internode length and the second panel length, and difference is aobvious compared with the control
Write, inhibiting rate be respectively (16.58%, 27.95% and 18.42%), (18.53%, 36.87% and 23.57%) and
(18.82%, 33.92% and 26.73%)；Plant Height in Wheat and height of C.G. reduce, and between base portion Section 1 and the second panel length is long
Degree shortens, and stalk mechanical strength enhancing, lodging coefficient reduces, to improve the lodging resistance of wheat plant.Cycocel individually makes
When used in 100mg/L and 200mg/L, inhibition Plant Height in Wheat, first internode length and the second panel length, inhibiting rate are respectively
(13.90%, 22.98% and 8.77%) and (14.90%, 28.70% and 13.74%)；Cycocel is used alone, and can also reduce
Plant Height in Wheat improves wheat lodging tolerance, but function and effect are lower than 14- hydroxyl brassinosteroids and cycocel compounding agent.
Influence of the table 9-1 compounding agent to Plant Height in Wheat and panel length
9.2.2 the influence handled wheat is respectively handled
By table 9-2 it is found that 14- hydroxyl brassinosteroids and cycocel compounding agent are in 50mg/L, 100mg/L and 200mg/L
When, wheat spike length, spike number and grain number per spike increase, compared with the control, growth rate be respectively (2.47%, 1.87% and 7.37%),
(3.64%, 3.51% and 3.67%) and (4.68%, 6.54% and 9.29%), while increasing the mass of 1000 kernel of wheat, and compares
It compares, growth rate is respectively 3.26%, 3.67% and 4.18%, and when wherein concentration is 200mg/L, effect of increasing production is preferable.It is short strong
Element is used alone in 100mg/L and 200mg/L, shows facilitation to wheat spike length, spike number and grain number per spike, and to photograph
It is respectively (1.73%, 1.73% and 4.09%) and (2.64%, 0.96% and 6.94%) than growth rate, to thousand grain weight of wheat
Growth rate is 1.61% and 2.23%.
Influence of the table 9-2 compounding agent to wheat yield
9.3 conclusion
Test result shows that 14- hydroxyl brassinosteroids and cycocel compounding agent and cycocel are used alone, Plant Height in Wheat
It reduces with height of C.G., shortens between base portion Section 1 with the second panel length length, the enhancing of stalk mechanical strength, lodging coefficient drop
It is low, to improve the lodging resistance of wheat plant；Wheat spike length, spike number and grain number per spike increase, while increasing the mass of 1000 kernel of wheat,
Play effect of increasing production.But it is comprehensively compared, 14- hydroxyl brassinosteroids and cycocel compounding agent is resistant to lodging to wheat and production-increasing function
Effect, better than cycocel is used alone, wherein for concentration in 100mg/L~200mg/L, using effect is preferable.
Embodiment 10:14- hydroxyl brassinosteroids and gibberellin compounding agent influence grape growth
10.1 materials and methods
10.1.1 test material
Material to be tested: 5 years Sheng Xiahei grape varieties in luxuriant and rich with fragrance grape villa garden are drawn in Zhongmou County.Orchard soil property is loam, seeding row spacing
For 1.0cm × 2.5cm, arch pergola system, tree vigo(u)r is medium, and underground is poured water, and management level is good, field clay fertilizer water and pest and disease damage
Prevention and treatment is equivalent conventional.
Test medicine: compounding agent (+2.9% gibberellin of 0.1%14- hydroxyl brassinosteroids), 3% gibberellin.
10.1.2 test method
10 plants of test are a cell, and random district's groups arrangement is repeated 3 times.Select growth potential, the comparable plant of diameter of fruit branch
Strain is as test strain, and the flower spike listing mark almost the same to size, every plant 10 listed, unified field management.
Field trial uses spraying treatment, and 50mg/L, 80mg/L is arranged in 14- hydroxyl brassinosteroids and gibberellin compounding agent
With 3 concentration of 100mg/L, 2 concentration of 80mg/L and 100mg/L are arranged in 3% gibberellin, if clear water compares CK.It is right before blooming
Flower spike sprays 1 time, and Post flowering sprays young fringe 1 time.
10.1.3 data determination
In grape maturity harvesting, the fruit ear being listed when to application measures Ear-Length, single fruit fringe weight, single fruit grain
Weight (respectively taking 5 measurements, averaged from fruit ear base portion, middle part and tip), and record statistical analysis.Test data DPS
(trial edition) and Excel software carry out correlation statistical analysis.
10.2 interpretations of result
By table 10-1 it is found that 14- hydroxyl brassinosteroids and gibberellin compounding agent are in 50mg/L, 80mg/L and 100mg/L
When, facilitation is showed to grape fruit ear length, simple grain grape weight and single fruit fringe weight, compared with the control, growth rate is respectively
(15.12%, 15.19% and 17.72%), (19.84%, 19.01% and 36.08%) and (15.75%, 19.66% and
28.48%)；Gibberellin is used alone in 80mg/L and 100mg/L, to grape fruit ear length, simple grain grape weight and single fruit fringe
Weight show facilitation, compared with the control, growth rate be respectively (9.13%, 13.79% and 10.13%) and (12.60%,
14.35% and 13.92%).It is found by growth rate score, gibberellin is used alone in 100mg/L, with compounding agent in 50mg/L
When growth rate maintain an equal level, but be lower than growth rate of the compounding agent in 80mg/L and 100mg/L.
The influence of table 10-1 compounding agent and gibberellin to grape growth
10.3 conclusions
Test result shows that 14- hydroxyl brassinosteroids and gibberellin compounding agent and gibberellin are used alone, set dense
Degree remarkably promotes effect to grape fruit ear length, simple grain grape weight and the performance of single fruit fringe weight, there is apparent effect of increasing production.It is comprehensive
Composition and division in a proportion is substantially better than exclusive use gibberellin to the effect of increasing production of grape compared with, 14- hydroxyl brassinosteroids and gibberellin compounding agent,
Wherein for concentration in 80mg/L~100mg/L, using effect is preferable.
Claims (9)
1. the preparation of a plant growth regulators, it is characterised in that: the plant growth regulator is 14- hydroxyl brassin steroid
Alcohol；The plant growth regulator 14- hydroxyl brassinosteroids are raw material by adding hydrogen or going back using moulting hormone and its derivative
Original method is synthesized；Catalyst is metallic catalyst, and catalyst includes lithium amide, tetrahydrochysene lithium aluminium, sodium borohydride/cuprous iodide
Or Pd/C, Pt/C, Pd/ (OH)2Or raney nickel.
2. the preparation of plant growth regulator according to claim 1, it is characterised in that: solvent for use is acetic acid second in preparation
Ester, ethyl alcohol, propyl alcohol, isopropanol, tetrahydrofuran (THF) or dioxane (dioxanes)；The reaction temperature of preparation is -78 DEG C~30
℃。
3. the preparation of plant growth regulator according to claim 2, it is characterised in that: the plant growth regulator system
The dosage form of agent is aqueous emulsion, aqua, milk powder agent or pulvis；The pulvis includes soluble powder or wettable powder.
4. the preparation of plant growth regulator according to claim 3, it is characterised in that: the 14- hydroxyl brassin steroid
The mass percentage content of alcohol in the formulation is 0.0001%~10%.
5. the preparation of plant growth regulator according to claim 2 or 3, it is characterised in that: the preparation can individually make
With or with cell classification element class, gibberellic acid class, fungicide, plant inhibition class regulator, classes of herbicides, abscisic acid and biochemical class tune
Agent compounding is saved, by weight percentage including following components: 14- hydroxyl brassinosteroids 0.0001~1.0%, auxiliary agent 85~
99.9%；The auxiliary agent is solvent, emulsifier and synergist, and the auxiliary agent includes following components by weight percentage: including
Deionized water 55~95%, solvent 5~15%, synergist 5~15%.
6. the preparation of plant growth regulator according to claim 5, it is characterised in that: the emulsifier is at least one
Selected from neopelex, alkyl phenol polyoxyethylene ether, Ben-zylphenol Polyoxyethyl Ether, phenethyl phenol polyethenoxy ether, alkane
Base sulfonate, alkyl phenol polyoxyethylene ether phosphate, aliphatic alcohol polyoxyvinethene phosphate；The solvent is selected from least one
Methanol, ethyl alcohol, propyl alcohol, isopropanol, acetone, cyclopentanone, cyclohexanone or ethyl acetate；Synergist is at least one beneficial selected from adding
Powder, anhydrous magnesium sulfate, zinc sulfate, calcium polyaspartate；The filler is at least one selected from white carbon black, kaolin, diatomite
Or precipitated calcium carbonate；The anticaking agent is potassium ferrocyanide or silica.
7. preparation made from the preparation to plant growth regulating composition described in 6 any one is promoting plant according to claim 1
Growth promotes fruits and vegetables vernalization, bears fruit, the application of improving quality and degeneration-resistant aspect.
8. application according to claim 7, it is characterised in that: the fruits and vegetables include fruits and vegetables；The fruit is to appoint
It anticipates a kind of selected from grape, citrus, lichee, apple, pears, longan, loquat, plum, strawberry, banana, cherry, mango, Kiwi berry, peach
Or apricot；Watermelon, muskmelon, "Hami" melon etc. be melon, the vegetables are that any one is selected from tomato, cucumber, sponge gourd, long bean, eggplant,
Balsam pear, radish, carrot, garden pea, pea, green pepper or capsicum, green onion, garlic, ginger, leek, beet, potato, pachyrhizus, taro and
Leafy vegetable.
9. application according to claim 8, it is characterised in that: the application further include tealeaves, tobacco, cotton, soybean,
Application in terms of rape, peanut, mung bean, rice, wheat, corn, flowers, Chinese medicine, lawn.
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