CN110478514A - A kind of anti-HPV viruse dressing and preparation method thereof - Google Patents
A kind of anti-HPV viruse dressing and preparation method thereof Download PDFInfo
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- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/408—Virucides, spermicides
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/416—Anti-neoplastic or anti-proliferative or anti-restenosis or anti-angiogenic agents, e.g. paclitaxel, sirolimus
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Abstract
The present invention relates to dressing technical fields, and in particular to a kind of anti-HPV viruse dressing and preparation method thereof, the adjuster of the poly- polysaccharide in sulphation yeast Portugal of the invention as enhancing Cellular Immunity and humoral immunity can effectively improve anti-HPV effect.Particularly, when phytic acid ketone, flavones, silicone is used in combination in poly- polysaccharide when sulphation yeast Portugal, there is obvious inhibiting effect for HPV gene masculine cervical cancer cell growth.
Description
Technical field
The present invention relates to dressing technical fields, and in particular to a kind of anti-HPV viruse dressing and preparation method thereof.
Background technique
Currently, cervical carcinoma is to endanger the primary killers of women's health.Research shows that can be examined in 90% or more cervical carcinoma
HPVDNA is measured, this is also that World Health Organization's confirmation cervical carcinoma is the material evidence as caused by HPV.HPV viruse is a kind of cream
The papilloma vacuolating virus A of more sky Viraceaes belongs to, and is spherical double-stranded DNA virus, can cause the squama of human skin and mucosal epithelium
Columnar epithelium proliferation.It can cause symptom of multiplicity, including thorn tumor, wart, genitals condyloma etc..
China's cervical cancer pathogenesis rate occupy the second in the world, and is in rejuvenation trend, there is nearly 150,000 new cases every year,
Account for about the 1/3 of world patient, therefore about 80,000 people die.And the mankind are the unique hosts of HPV viruse, from infection HPV viruse to development
At least need ten years at cervical carcinoma, wherein virus contacted with cell be HPV infection host necessity and prerequisite；Period
By infection people's mucous epithelium, epithelium damage generate wound, viruses contact and enter cell (prevention and remove best period),
Virus maturation simultaneously discharges the more tissue epithelials of infection, further results in wound expansion, eventually leading to exacerbation makes to exempt from
Epidemic disease system can not remove HPV viruse and form wart, cervical erosion, cervical carcinoma etc..
Currently, generally acknowledged prevention HPV viruse most effective way is injection HPV viruse vaccine both at home and abroad, by large area
Clinical trial verifying HPV vaccine can prevent the infection of some HPV viruses really, but expensive.There are also other anti-HPV
The relevant technologies of virus, such as application No. is 03111091.6 ointment against virus of human papilloma patents to describe a kind of anti-human nipple
Tumor virus ointment, wherein containing arsenolite (be the raw material for preparing hypertoxic arsenic, it is dangerous there are poisoning risk.For another example application No. is
CN201410798607.9 discloses a kind of anti-HPV viruse gel dressing, including anti-HPV viruse freeze-dried powder and lysate；It is anti-
HPV viruse freeze-drying powder component specifically includes boheic acid albumin A P 0.01-10%, different poly- maltose 1-25%, freeze drying protectant
20-90% ,/bacteriostatic agent 0.01-1% is killed；The lysate component includes water 60-100%, phosphate 0-2%, glycerol 0-
15%, peppermint oil 0-8%, but effect is unobvious.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to and provide it is a kind of safely, effectively, have no toxic side effect it is anti-
HPV viruse dressing and preparation method thereof.
The present invention is achieved through technology: a kind of anti-HPV viruse dressing: it is poly- with Sulfation yeast Portugal
Sugar is active constituent.
Preferably, the modification rate 15%-25% of the Sulfation yeast dextran sulfate radical.
Preferably, the Sulfation yeast dextran is used cooperatively with three kinds of phytic acid ketone, silicone and flavones ketones.
Preferably, Sulfation yeast dextran, phytic acid ketone, silicone, flavones weight ratio be (1-50): (10-200):
(1-10): (5-50).
Preferably, this kind of anti-HPV viruse dressing comprises the following raw materials by weight percent:
Sulfation yeast dextran 0.01%-0.5%
Phytic acid ketone 0.1%-2%
Silicone 0.01%-0.1%
Flavones 0.05%-0.5%
Hypromellose 0.5%-2%
Glycerol 1%-5%
A.SAP 0.01%-0.5%
Purify water surplus.
A kind of preparation method of anti-HPV viruse dressing, it the following steps are included:
Step 1: the purifying of sulphation yeast the Portugal poly- polysaccharide, phytic acid ketone, glycerol, A.SAP and half formula ratio of formula ratio is weighed
Water stirs to get lotion；
Step 2: weighing the purified water of remaining formula ratio, is heated to 70 °C or more, and silicone, the hydroxypropyl that formula ratio is added are fine
Dimension element is emulsified, and after forming mutually stable lotion, is poured into the lotion of step 1 and is continued to stir evenly to get a kind of anti-HPV
Viral dressing.
Preferably, the Sulfation yeast dextran is that 70-75g urea is dissolved completely in 100ml dimethyl sulfoxide
In, 2-8g yeast dextran is added, after being completely dissolved, the mixed solution of the 10ml concentrated sulfuric acid and 100ml dimethyl sulfoxide, In is added
5-7h is reacted at a temperature of 115-125 °C, obtains Sulfation yeast dextran solution.
Preferably, Sulfation yeast dextran solution is centrifuged at a temperature of 3-6oC, remove supernatant, then be pre-chilled
The normal propyl alcohol washing crossed precipitating 2-4 times；Precipitating uses deionized water dissolving again, and the supernatant soln of acquisition is freeze-dried by centrifugation,
The poly- polysaccharide in sulphation yeast Portugal can be obtained.
By the yeast dextran of Sulfation, water solubility enhancing, the anti-tumor activity of anti-HPV can also be increased altogether
By force.The poly- polysaccharide in Sulfation yeast Portugal can be combined with Toll receptor, and activated macrophage and inducing generates white thin in vitro
Born of the same parents' interleukin -6(IL-6) and tumor necrosis factor, T cell and natural killer cells, promote T lymphocyte differentiation, activation, in turn
The complement variation of Alternative pathway is influenced, so that the adjuster as enhancing Cellular Immunity and humoral immunity, is improved anti-
HPV effect.
Phytic acid ketone, flavones, silicone, which are used in combination, has obvious inhibiting effect for HPV gene masculine cervical cancer cell growth.
They can change environment in the pH value of HPV existence, make HPV viruse Yi KedanbaiL1 L2 degeneration necrosis, to block its duplication
Process；Promote the small Wound healing of cervical surface, HPV is blocked to enter host cell；It induces body to generate HPV to be immunized, to prevent
Isotype subclass subinfection again.Phytic acid ketone, flavones, silicone can effectively be cooperated using A.SAP antibacterial agent, enhance the antibiotic property of dressing
Energy.
Using the viscosity and lubricity of hypromellose and glycerol enhancing dressing, HPV patient's use feeling is improved.
Beneficial effects of the present invention: the poly- polysaccharide in sulphation yeast Portugal of the invention is as enhancing Cellular Immunity and body fluid
Immune adjuster can effectively improve anti-HPV effect.Particularly, when sulphation yeast Portugal poly- polysaccharide be used in combination phytic acid ketone,
When flavones, silicone, there is obvious inhibiting effect for HPV gene masculine cervical cancer cell growth.
Detailed description of the invention
Fig. 1 is the experimental result schematic diagram of 96 hole microtest plates after 7 days of the embodiment of the present invention.
Specific embodiment
For the ease of the understanding of those skilled in the art, below with reference to embodiment, the present invention is further illustrated, real
The content that the mode of applying refers to not is limitation of the invention.
Embodiment 1
A kind of anti-HPV viruse dressing: it is using Sulfation yeast dextran as active constituent.
The modification rate 15% of the Sulfation yeast dextran sulfate radical.
The Sulfation yeast dextran is used cooperatively with three kinds of phytic acid ketone, silicone and flavones ketones.
This kind of anti-HPV viruse dressing comprises the following raw materials by weight percent:
Sulfation yeast dextran 0.01%
Phytic acid ketone 2%
Silicone 0.1%
Flavones 0.5%
Hypromellose 0.5%
Glycerol 1%
A.SAP 0.01%
Purify water surplus.
A kind of preparation method of anti-HPV viruse dressing, it the following steps are included:
Step 1: the purifying of sulphation yeast the Portugal poly- polysaccharide, phytic acid ketone, glycerol, A.SAP and half formula ratio of formula ratio is weighed
Water stirs to get lotion；
Step 2: weighing the purified water of remaining formula ratio, is heated to 70 °C or more, and silicone, the hydroxypropyl that formula ratio is added are fine
Dimension element is emulsified, and after forming mutually stable lotion, is poured into the lotion of step 1 and is continued to stir evenly to get a kind of anti-HPV
Viral dressing.
The Sulfation yeast dextran is that 70g urea is dissolved completely in 100ml dimethyl sulfoxide, and 2g ferment is added
After being completely dissolved, the mixed solution of the 10ml concentrated sulfuric acid and 100ml dimethyl sulfoxide is added in female glucan, anti-at a temperature of 115 °C
7h is answered, Sulfation yeast dextran solution is obtained.
Sulfation yeast dextran solution is centrifuged at a temperature of 6oC, removes supernatant, then with precooled normal propyl alcohol
Washing precipitating 4 times；Precipitating uses deionized water dissolving again, and the supernatant soln of acquisition is freeze-dried, sulfuric acid can be obtained by centrifugation
Change the poly- polysaccharide in yeast Portugal.
Embodiment 2
A kind of anti-HPV viruse dressing: it is using Sulfation yeast dextran as active constituent.
The modification rate 20% of the Sulfation yeast dextran sulfate radical.
This kind of anti-HPV viruse dressing comprises the following raw materials by weight percent:
Sulfation yeast dextran 0.2%
Phytic acid ketone 1%
Silicone 0.05%
Flavones 0.3%
Hypromellose 1%
Glycerol 2%
A.SAP 0.3%
Purify water surplus.
A kind of preparation method of anti-HPV viruse dressing, it the following steps are included:
Step 1: the purifying of sulphation yeast the Portugal poly- polysaccharide, phytic acid ketone, glycerol, A.SAP and half formula ratio of formula ratio is weighed
Water stirs to get lotion；
Step 2: weighing the purified water of remaining formula ratio, is heated to 70 °C or more, and silicone, the hydroxypropyl that formula ratio is added are fine
Dimension element is emulsified, and after forming mutually stable lotion, is poured into the lotion of step 1 and is continued to stir evenly to get a kind of anti-HPV
Viral dressing.
The Sulfation yeast dextran is that 72g urea is dissolved completely in 100ml dimethyl sulfoxide, and 6g ferment is added
After being completely dissolved, the mixed solution of the 10ml concentrated sulfuric acid and 100ml dimethyl sulfoxide is added in female glucan, anti-at a temperature of 120 °C
6h is answered, Sulfation yeast dextran solution is obtained.
Sulfation yeast dextran solution is centrifuged at a temperature of 5oC, removes supernatant, then with precooled normal propyl alcohol
Washing precipitating 3 times；Precipitating uses deionized water dissolving again, and the supernatant soln of acquisition is freeze-dried, sulfuric acid can be obtained by centrifugation
Change the poly- polysaccharide in yeast Portugal.
Embodiment 3
A kind of anti-HPV viruse dressing: it is using Sulfation yeast dextran as active constituent.
The modification rate 23% of the Sulfation yeast dextran sulfate radical.
The Sulfation yeast dextran is used cooperatively with three kinds of phytic acid ketone, silicone and flavones ketones.
This kind of anti-HPV viruse dressing comprises the following raw materials by weight percent:
Sulfation yeast dextran 0.4%
Phytic acid ketone 1.5%
Silicone 0.06%
Flavones 0.3%
Hypromellose 1.5%
Glycerol 2%
A.SAP 0.5%
Purify water surplus.
A kind of preparation method of anti-HPV viruse dressing, it the following steps are included:
Step 1: the purifying of sulphation yeast the Portugal poly- polysaccharide, phytic acid ketone, glycerol, A.SAP and half formula ratio of formula ratio is weighed
Water stirs to get lotion；
Step 2: weighing the purified water of remaining formula ratio, is heated to 70 °C or more, and silicone, the hydroxypropyl that formula ratio is added are fine
Dimension element is emulsified, and after forming mutually stable lotion, is poured into the lotion of step 1 and is continued to stir evenly to get a kind of anti-HPV
Viral dressing.
The Sulfation yeast dextran is that 72g urea is dissolved completely in 100ml dimethyl sulfoxide, and 6g ferment is added
After being completely dissolved, the mixed solution of the 10ml concentrated sulfuric acid and 100ml dimethyl sulfoxide is added in female glucan, anti-at a temperature of 120 °C
6h is answered, Sulfation yeast dextran solution is obtained.
Sulfation yeast dextran solution is centrifuged at a temperature of 4oC, removes supernatant, then with precooled normal propyl alcohol
Washing precipitating 3 times；Precipitating uses deionized water dissolving again, and the supernatant soln of acquisition is freeze-dried, sulfuric acid can be obtained by centrifugation
Change the poly- polysaccharide in yeast Portugal.
Embodiment 4
A kind of anti-HPV viruse dressing: it is using Sulfation yeast dextran as active constituent.
The modification rate 25% of the Sulfation yeast dextran sulfate radical.
This kind of anti-HPV viruse dressing comprises the following raw materials by weight percent:
Sulfation yeast dextran 0.5%
Phytic acid ketone 0.1%%
Silicone 0.01%
Flavones 0.5%
Hypromellose 2%
Glycerol 5%
A.SAP 0.5%
Purify water surplus.
A kind of preparation method of anti-HPV viruse dressing, it the following steps are included:
Step 1: the purifying of sulphation yeast the Portugal poly- polysaccharide, phytic acid ketone, glycerol, A.SAP and half formula ratio of formula ratio is weighed
Water stirs to get lotion；
Step 2: weighing the purified water of remaining formula ratio, is heated to 70 °C or more, and silicone, the hydroxypropyl that formula ratio is added are fine
Dimension element is emulsified, and after forming mutually stable lotion, is poured into the lotion of step 1 and is continued to stir evenly to get a kind of anti-HPV
Viral dressing.
The Sulfation yeast dextran is that 75g urea is dissolved completely in 100ml dimethyl sulfoxide, and 8g ferment is added
After being completely dissolved, the mixed solution of the 10ml concentrated sulfuric acid and 100ml dimethyl sulfoxide is added in female glucan, anti-at a temperature of 125 °C
7h is answered, Sulfation yeast dextran solution is obtained.
Sulfation yeast dextran solution is centrifuged at a temperature of 6oC, removes supernatant, then with precooled normal propyl alcohol
Washing precipitating 4 times；Precipitating uses deionized water dissolving again, and the supernatant soln of acquisition is freeze-dried, sulfuric acid can be obtained by centrifugation
Change the poly- polysaccharide in yeast Portugal.
The active inhibiting effect of HPV viruse is tested in the anti-HPV viruse dressing of the embodiment of the present invention.
(1) 100 μ l of cell suspension is added in 96 hole microtest plates, cell concentration is made to reach 2~3x105/ml.Wherein 1
Tandem is normal cell growth blank control (+100 μ l cell suspension of 100 μ l growth-promoting media)；
+ 100 μ l cell suspension of+50 μ l growth-promoting media of 50 μ lHPV16 pseudovirus is added in (2) 2 tandems, as positive control；
(3) continuous 10 times of dilution gradient is made into anti-HPV viruse dressing in 1.5ml centrifuge tube, from 10-1~10-3It will dilute
Anti- HPV viruse dressing be inoculated into 96 hole microtest plates, each dilution gradient is inoculated with 2 tandems totally 6 tandems (number 3~8),
Every hole is inoculated with 50 μ l, inoculates 50 μ lHPV16 pseudovirus, as test group, investigates anti-HPV viruse dressing to HPV16 pseudovirus
Active inhibiting effect；
(4) 9,10,11 tandems addition 10-1~10-3The 50 μ l of anti-HPV viruse dressing of each dilution gradient adds 50 μ l growth
Whether+100 μ l cell suspension of liquid, investigating anti-HPV viruse dressing has inhibiting effect to the growth of cell suspension；
(4) by 96 hole microtest plates in 37oC, 5%CO2It is cultivated 7 days in incubator, observes and records cytopathy effect occur daily
The hole count answered.
The experimental result of 96 hole microtest plates is as shown in Figure 1 after 7 days.
1 tandem and 9~11 tandems the experimental results showed that, anti-HPV viruse dressing does not inhibit to make for the growth of cell
With；There is pathological effect in the cell suspension that 2 tandems have infected HPV16 pseudovirus；Then 3~8 tandems joined anti-HPV simultaneously
The cell suspension cell growth condition of viral dressing and HPV16 pseudovirus is good, shows anti-HPV viruse dressing for HPV viruse
Activity has certain inhibiting effect.
The above is only a preferred embodiment of the present invention, for those of ordinary skill in the art, according to the present invention
Thought, there will be changes in the specific implementation manner and application range, and the content of the present specification should not be construed as to the present invention
Limitation.
Claims (8)
1. kind of anti-HPV viruse dressing, it is characterised in that: it is using Sulfation yeast dextran as active constituent.
2. a kind of anti-HPV viruse dressing according to claim 1, it is characterised in that: the Sulfation yeast dextran
The modification rate of middle sulfate radical is in 15%-25%.
3. a kind of anti-HPV viruse dressing according to claim 1, it is characterised in that: the Sulfation yeast dextran
It is used cooperatively with three kinds of phytic acid ketone, silicone and flavones ketones.
4. a kind of anti-HPV viruse dressing according to claim 1, it is characterised in that: Sulfation yeast dextran, phytic acid
Ketone, silicone, flavones weight ratio be (1-50): (10-200): (1-10): (5-50).
5. a kind of anti-HPV viruse dressing according to claim 1, it is characterised in that: this kind of anti-HPV viruse dressing is by following
The raw material of weight percent forms:
Sulfation yeast dextran 0.01%-0.5%
Phytic acid ketone 0.1%-2%
Silicone 0.01%-0.1%
Flavones 0.05%-0.5%
A.SAP 0.01%-0.5%
Hypromellose 0.5%-2%
Glycerol 1%-5%
Purify water surplus.
6. a kind of preparation method of anti-HPV viruse dressing as claimed in claim 5, it is characterised in that: it the following steps are included:
Step 1: the purifying of sulphation yeast the Portugal poly- polysaccharide, phytic acid ketone, glycerol, A.SAP and half formula ratio of formula ratio is weighed
Water stirs to get lotion；
Step 2: weighing the purified water of remaining formula ratio, is heated to 70 °C or more, and silicone, the hydroxypropyl that formula ratio is added are fine
Dimension element is emulsified, and after forming mutually stable lotion, is poured into the lotion of step 1 and is continued to stir evenly to get a kind of anti-HPV
Viral dressing.
7. a kind of preparation method of anti-HPV viruse dressing according to claim 6, it is characterised in that: the Sulfation
Yeast dextran is that 70-75g urea is dissolved completely in 100ml dimethyl sulfoxide, and 2-8g yeast dextran is added, completely molten
Xie Hou is added the mixed solution of the 10ml concentrated sulfuric acid and 100ml dimethyl sulfoxide, reacts 5-7h at a temperature of 115-125 °C, obtain
Sulfation yeast dextran solution.
8. a kind of preparation method of anti-HPV viruse dressing according to claim 7, it is characterised in that: by Sulfation ferment
Female dextran solution is centrifuged at a temperature of 3-6oC, removes supernatant, then wash precipitating 2-4 times with precooled normal propyl alcohol；Precipitating
Deionized water dissolving is used again, and the supernatant soln of acquisition is freeze-dried, the poly- polysaccharide in sulphation yeast Portugal can be obtained by centrifugation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910527135.6A CN110478514A (en) | 2019-06-18 | 2019-06-18 | A kind of anti-HPV viruse dressing and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910527135.6A CN110478514A (en) | 2019-06-18 | 2019-06-18 | A kind of anti-HPV viruse dressing and preparation method thereof |
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| CN110478514A true CN110478514A (en) | 2019-11-22 |
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