CN110476804A - A method of promoting ginseng adventitious root - Google Patents
A method of promoting ginseng adventitious root Download PDFInfo
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- CN110476804A CN110476804A CN201810192824.1A CN201810192824A CN110476804A CN 110476804 A CN110476804 A CN 110476804A CN 201810192824 A CN201810192824 A CN 201810192824A CN 110476804 A CN110476804 A CN 110476804A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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Abstract
The invention discloses a kind of methods for promoting ginseng adventitious root to occur; it include: to prepare culture medium, optimization culture, nutrition culture; it is basic culture medium with MS culture medium, adds tender willow juice, sucrose, heteroauxin, protective agent, rice vinegar, aspirin, vitamin B12 and obtain Optimal Medium；It is basic culture medium with MS culture medium, addition methyl α-naphthyl acetate, indolebutyric acid, amine fresh fat, D-malic acid, aloe mannosan obtain nutrient medium；Ginseng seeds after disinfection are successively obtained into adventitious root after MS culture medium, Optimal Medium, nutrient medium are cultivated respectively.Have the beneficial effect that, by optimization culture, nutrition culture, more adventitious roots can be born, ginseng item number increases, diameter increases, length increases, and can greatly improve the survival rate of ginseng after transplanting.
Description
Technical field
The present invention relates to plant cultivation fields, more particularly, to a kind of method for promoting ginseng adventitious root to occur.
Technical background
Ginseng (Panax ginseng C. A. Mey) is herbaceos perennial, and happiness is shady and cool, blade pore-free and fence
Tissue can not retain moisture, and temperature, which is higher than 32 degree of blades, to burn, canopy density 0.7-0.8.It blooms within usual 3 years, 5-6 annual bearing,
The month at florescence 5-6, the fruiting period 6-9 month.The wealthy mixed friendship of hundreds of meters of height above sea level of the needle based on Korean pine being grown between 33 degree -48 of north latitude degree
Woods or broad leaved and deciduous broad leaved hayashishita originate in Northeast China, Korea, South Korea, Japan, Russian east.The nickname of ginseng be kurarinone,
Essence, god grass, King of Herbs are one of well-known " northeast Triratnas ".Ginseng be described as from ancient times " King of Herbs " " enriching yin mend life,
Strengthen the body resistance to consolidate the constitution " superfine product, contain a variety of saponins and polysaccharide component, leachate can be by skin slow-absorbing and without pessimal stimulation, energy
Capillary of skin is expanded, promotes skin blood circulation, increases skin-nourishing, adjust the water and oil balance of skin, prevents skin de-
Water, hardening, corrugation, ginseng active substance inhibit the reducing property of melanin, keep skin pure white smooth, can enhance skin elasticity,
It obtains cell newborn, is the superfine product improved looks.The fleshy root of ginseng be famous strong tonic, be suitable for adjustment blood pressure,
Restore cardiac function, neurasthenia and the diseases such as in poor health, also there is eliminating the phlegm, stomach invigorating, diuresis, excitement and other effects.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for promoting ginseng adventitious root to occur, and this method is simple and easy, promotes
The culture medium activity that ginseng adventitious root occurs is kept preferably, and ginseng rooting rate is fast, and ginseng item number is more, diameter is sturdy, transplanting
Survival rate is higher afterwards.
The present invention in background technique aiming at the problem that mentioning, the technical solution taken are as follows: one kind promotes ginseng adventitious root to send out
Raw method, comprising: culture medium, optimization culture, nutrition culture are prepared, specifically includes the following steps:
It prepares culture medium: preparing Optimal Medium are as follows: the tender willow juice of basal medium, 16-19g/L, 70-80g/L sucrose, 1.2-
1.5mg/L heteroauxin, 15-25mg/L protective agent, 3-4g/L rice vinegar, 0.15-0.25g/L aspirin, 0.10-0.12g/L
Vitamin B12 is sterilized and is saved；Prepare nutrient medium are as follows: basal medium, 0.5-0.6mg/L methyl α-naphthyl acetate, 1.0-1.4mg/
L indolebutyric acid, 30-50mg/L amine fresh fat, 2.5-2.8g/L D-malic acid, 1.5-2.0g/L aloe mannosan, sterilize and protect
It deposits；Basal medium is MS culture medium；Culture solution can promote root system endoperoxides object enzyme, polyphenol oxidase, heteroauxin oxygen
The activity change for changing the enzymes such as enzyme also helps the induction of root initial body, and the activity of stimulation cambial cell simultaneously promotes cell dedifferentiation,
Callus is generated, inspires new root, new root is not limited only to the cortical cell around vascular bundle, cortex and is similar to center pillar taper
The cell of position can break up and form root restriction, therefore newborn well developed root system, sturdy, be suitable for and survive；Salicylic acid, pulullan polysaccharide
Adventitious root bioactive substance content can be significantly improved, while improving the antioxygenic property of adventitious root water body object, it is indefinite to enhance
The disease-resistant antibacterial ability of root improves adventitious root survival rate；
Optimization culture: the ginseng seeds after disinfection are inoculated into the culture dish of the culture medium containing MS, carry out the induction of adventitious root, will
The fritter that 0.5-1.2cm length is cut into after the adventitious root disinfection induced is stand-by；Then ginseng fritter is inoculated with into containing excellent
Change the balloon-type airlift bioreactor of culture medium, half-light ventilation culture at a temperature of 23-26 DEG C；It is induced in advance with MS culture medium
Optimization culture induction is carried out after taking root, and help to obtain the preferable new root of activity, the disease-resistant antibacterial ability of new root is promoted, at
Motility rate is higher；
Nutrition culture: it cultivates to after 10-15 days, nutrient medium is slowly added into bioreactor, in 23-26 DEG C of temperature
Lower half-light ventilation culture, adventitious root can be harvested in 20-30 days by continuing culture；Nutrition culture can promote on the basis of optimization culture
More adventitious roots are born, increases ginseng item number, diameter increase, length growth, ginseng after transplanting can be greatly improved
Survival rate.
Preferably, protective agent is a kind of Functional Polypeptides, the amino acid sequence of Functional Polypeptides are as follows:
SCASRCCRAFRCKSRCRARRCRARRCGCRARRCGYYVSVFRC；Heteroauxin can be oxidized to rose under light,
Its bioactivity can decline in cliff of displacement formula, and the Functional Polypeptides in system can produce stable compound with auxin, into plant
Become a kind of storage form of heteroauxin in object, so as to avoid heteroauxin turns to indoles by photooxidation and loses biology
Effect, the dissociated free state heteroauxin that can generate is absorbed and utilized for plant in plant, plays heteroauxin
Biological effectiveness.
Preferably, disinfection are as follows: half-light, 115-121 DEG C, sterilize 10-15 minute under the conditions of 0.3-0.5MPa, after cooling down secretly
Light saves；Disinfection can reduce pollution of the microorganism to culture medium, can also weaken erosion of the microorganism to ginseng, and half-light is protected
Deposit the bioactivity for helping to maintain auxin in culture medium.
Compared with the prior art, the advantages of the present invention are as follows:
1) Functional Polypeptides in system can produce stable compound with auxin, into plant in become heteroauxin one
Kind storage form, it is dissociated in plant so as to avoid heteroauxin turns to indoles by photooxidation and loses biological effectiveness
Free state heteroauxin can be generated to be absorbed and utilized for plant, play the biological effectiveness of heteroauxin；
2) culture solution can promote the activity change of the enzymes such as root system endoperoxides object enzyme, polyphenol oxidase, indoleacetic acid oxidase,
The induction of root initial body is also helped, the activity of stimulation cambial cell simultaneously promotes cell dedifferentiation, generates callus, inspires new
Root, new root are not limited only to the cortical cell around vascular bundle, cortex and the cell for being similar to center pillar taper position can break up and
It forms root restriction, therefore newborn well developed root system, sturdy, is suitable for and survives；
3) pass through optimization culture, nutrition culture, more adventitious roots can be born, and ginseng item number increases, diameter increases, length increases
It is long, the survival rate of ginseng after transplanting can be greatly improved.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A method of promoting ginseng adventitious root, comprising the following steps:
1) Optimal Medium is prepared are as follows: the tender willow juice of basal medium, 16g/L, 70g/L sucrose, 1.2mg/L heteroauxin, 15mg/
L protective agent, 3g/L rice vinegar, 0.15g/L aspirin, 0.10g/L vitamin B12 disappear under the conditions of half-light, 115 DEG C, 0.3MPa
Poison 10 minutes, half-light saves after cooling；Prepare nutrient medium are as follows: basal medium, 0.5mg/L methyl α-naphthyl acetate, 1.0mg/L indoles
Butyric acid, 30mg/L amine fresh fat, 2.5g/L D-malic acid, 1.5g/L aloe mannosan, sterilize and save；Basal medium is
MS culture medium；2) ginseng seeds after disinfection are inoculated into the culture dish of the culture medium containing MS, carry out the induction of adventitious root, will lured
The fritter that 0.5cm length is cut into after derived adventitious root disinfection is stand-by；Then ginseng fritter is inoculated with into containing optimization culture
The balloon-type airlift bioreactor of base, half-light ventilation culture at a temperature of 23 DEG C；3) it cultivates to after 10 days, by nutrient medium
It is slowly added into bioreactor, the half-light ventilation culture at a temperature of 23 DEG C, adventitious root can be harvested in 20 days by continuing culture；Battalion
Feeding culture can promote more adventitious roots to bear on the basis of optimization culture, increase ginseng item number, diameter increases, long
Degree increases, and can greatly improve the survival rate of ginseng after transplanting.
Embodiment 2:
A method of promoting ginseng adventitious root, comprising the following steps:
1) Optimal Medium is prepared are as follows: the tender willow juice of basal medium, 19g/L, 80g/L sucrose, 1.5mg/L heteroauxin, 25mg/
L protective agent, 4g/L rice vinegar, 0.25g/L aspirin, 0.12g/L vitamin B12 disappear under the conditions of half-light, 121 DEG C, 0.5MPa
Poison 15 minutes, half-light saves after cooling；Protective agent is a kind of Functional Polypeptides, the amino acid sequence of Functional Polypeptides are as follows:
SCASRCCRAFRCKSRCRARRCRARRCGCRARRCGYYVSVFRC；Heteroauxin can be oxidized to rose under light,
Its bioactivity can decline in cliff of displacement formula, and the Functional Polypeptides in system can produce stable compound with auxin, into plant
Become a kind of storage form of heteroauxin in object, so as to avoid heteroauxin turns to indoles by photooxidation and loses biology
Effect, the dissociated free state heteroauxin that can generate is absorbed and utilized for plant in plant, plays heteroauxin
Biological effectiveness；
2) prepare nutrient medium are as follows: basal medium, 0.6mg/L methyl α-naphthyl acetate, 1.4mg/L indolebutyric acid, 50mg/L amine fresh fat,
2.8g/L D-malic acid, 2.0g/L aloe mannosan, sterilize and save；
3) basal medium is MS culture medium；Culture solution can promote root system endoperoxides object enzyme, polyphenol oxidase, heteroauxin
The activity change of the enzymes such as oxidizing ferment, also helps the induction of root initial body, and the activity of stimulation cambial cell simultaneously promotes de- point of cell
Change, generate callus, inspire new root, new root is not limited only to the cortical cell around vascular bundle, cortex and is similar to center pillar
The cell of taper position can break up and form root restriction, therefore newborn well developed root system, sturdy, be suitable for and survive；Salicylic acid, Propiram
Polysaccharide can significantly improve adventitious root bioactive substance content, while improve the antioxygenic property of adventitious root water body object, enhancing
The disease-resistant antibacterial ability of adventitious root improves adventitious root survival rate；
4) optimization culture: the ginseng seeds after disinfection are inoculated into the culture dish of the culture medium containing MS, carry out the induction of adventitious root,
The fritter that 1.2cm length is cut into after the adventitious root induced disinfection is stand-by；Then ginseng fritter is inoculated with into containing optimization
The balloon-type airlift bioreactor of culture medium, half-light ventilation culture at a temperature of 26 DEG C；With the leading root induction of MS culture medium
Optimization culture induction is carried out afterwards, help to obtain the preferable new root of activity, and the disease-resistant antibacterial ability of new root is promoted, survival rate
It is higher；
5) nutrition culture: cultivating to after 15 days, nutrient medium be slowly added into bioreactor, dark at a temperature of 26 DEG C
Light ventilation culture, adventitious root can be harvested in 30 days by continuing culture；Nutrition culture can promote more on the basis of optimization culture
Adventitious root bear, increase ginseng item number, diameter increases, length increases, ginseng survives after can greatly improving transplanting
Rate.
Embodiment 3:
A method of promoting ginseng adventitious root, comprising: prepare culture medium, optimization culture, nutrition culture, specifically include with
Lower step:
It prepares culture medium: preparing Optimal Medium are as follows: the tender willow juice of basal medium, 18g/L, 75g/L sucrose, 1.4mg/L indoles
Acetic acid, 20mg/L protective agent, 3g/L rice vinegar, 0.20g/L aspirin, 0.10g/L vitamin B12, half-light, 120 DEG C,
It is sterilized 12 minutes under the conditions of 0.4MPa, half-light saves after cooling；Prepare nutrient medium are as follows: basal medium, 0.5mg/L naphthalene second
Acid, 1.3mg/L indolebutyric acid, 40mg/L amine fresh fat, 2.6g/L D-malic acid, 1.8g/L aloe mannosan, sterilize and protect
It deposits；Basal medium is MS culture medium；Culture solution can promote root system endoperoxides object enzyme, polyphenol oxidase, heteroauxin oxygen
The activity change for changing the enzymes such as enzyme also helps the induction of root initial body, and the activity of stimulation cambial cell simultaneously promotes cell dedifferentiation,
Callus is generated, inspires new root, new root is not limited only to the cortical cell around vascular bundle, cortex and is similar to center pillar taper
The cell of position can break up and form root restriction, therefore newborn well developed root system, sturdy, be suitable for and survive；Salicylic acid, pulullan polysaccharide
Adventitious root bioactive substance content can be significantly improved, while improving the antioxygenic property of adventitious root water body object, it is indefinite to enhance
The disease-resistant antibacterial ability of root improves adventitious root survival rate；
Optimization culture: the ginseng seeds after disinfection are inoculated into the culture dish of the culture medium containing MS, carry out the induction of adventitious root, will
The fritter that 1.0cm length is cut into after the adventitious root disinfection induced is stand-by；Then ginseng fritter is inoculated with into containing optimization training
The balloon-type airlift bioreactor of base is supported, half-light ventilation culture at a temperature of 24 DEG C；After the leading root induction of MS culture medium
Optimize culture induction, help to obtain the preferable new root of activity, the disease-resistant antibacterial ability of new root is promoted, survival rate compared with
It is high；
Nutrition culture: it cultivates to after 14 days, nutrient medium is slowly added into bioreactor, the half-light at a temperature of 25 DEG C
Ventilation culture, adventitious root can be harvested in 25 days by continuing culture；Nutrition culture can promote more on the basis of optimization culture
Adventitious root is born, and is increased ginseng item number, diameter increase, length growth, can be greatly improved the survival rate of ginseng after transplanting.
Protective agent is a kind of Functional Polypeptides, the amino acid sequence of Functional Polypeptides are as follows:
SCASRCCRAFRCKSRCRARRCRARRCGCRARRCGYYVSVFRC；Heteroauxin can be oxidized to rose under light,
Its bioactivity can decline in cliff of displacement formula, and the Functional Polypeptides in system can produce stable compound with auxin, into plant
Become a kind of storage form of heteroauxin in object, so as to avoid heteroauxin turns to indoles by photooxidation and loses biology
Effect, the dissociated free state heteroauxin that can generate is absorbed and utilized for plant in plant, plays heteroauxin
Biological effectiveness.
Routine operation in operation of the present invention step is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Sequence table
<110>Lanxi City silencing Biotechnology Co., Ltd
<120>a kind of method for promoting ginseng adventitious root to occur
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 42
<212> PRT
<213>artificial synthesized (Mytilus coruscus)
<400> 1
Ser Cys Ala Ser Arg Cys Cys Arg Ala Phe Arg Cys Lys Ser Arg Cys
1 5 10 15
Arg Ala Arg Arg Cys Arg Ala Arg Arg Cys Gly Cys Arg Ala Arg Arg
20 25 30
Cys Gly Tyr Tyr Val Ser Val Phe Arg Cys
35 40
Claims (8)
1. a kind of method for promoting ginseng adventitious root to occur, comprising: prepare culture medium, optimization culture, nutrition culture, feature exists
In: the preparation culture medium step are as follows:
Prepare Optimal Medium are as follows: the tender willow juice of basal medium, 16-19g/L, 70-80g/L sucrose, 1.2-1.5mg/L indoles second
Acid, 15-25mg/L protective agent, 3-4g/L rice vinegar, 0.15-0.25g/L aspirin, 0.10-0.12g/L vitamin B12, disinfection
And it saves；Prepare nutrient medium are as follows: basal medium, 0.5-0.6mg/L methyl α-naphthyl acetate, 1.0-1.4mg/L indolebutyric acid, 30-
50mg/L amine fresh fat, 2.5-2.8g/L D-malic acid, 1.5-2.0g/L aloe mannosan, sterilize and save.
2. a kind of method for promoting ginseng adventitious root to occur according to claim 1, it is characterised in that: the preparation culture
Basal medium in base step is MS culture medium.
3. a kind of method for promoting ginseng adventitious root to occur according to claim 1, it is characterised in that: the preparation culture
In base step, disinfection are as follows: half-light, 115-121 DEG C, sterilize 10-15 minute under the conditions of 0.3-0.5MPa, half-light preservation after cooling down.
4. a kind of method for promoting ginseng adventitious root to occur according to claim 1, it is characterised in that: the preparation culture
In base step, protective agent is a kind of Functional Polypeptides, the amino acid sequence of Functional Polypeptides are as follows:
SCASRCCRAFRCKSRCRARRCRARRCGCRARRCGYYVSVFRC。
5. a kind of method for promoting ginseng adventitious root to occur according to claim 1, it is characterised in that: the optimization culture
Step are as follows: the ginseng seeds after disinfection are inoculated into the culture dish of the culture medium containing MS, carry out the induction of adventitious root, will be induced
Adventitious root disinfection after be cut into 0.5-1.2cm length fritter it is stand-by；Then ginseng fritter is inoculated with into containing optimization culture
The balloon-type airlift bioreactor culture of base.
6. a kind of method for promoting ginseng adventitious root to occur according to claim 5, it is characterised in that: the optimization culture
Half-light ventilation culture at a temperature of condition of culture in step is 23-26 DEG C.
7. a kind of method for promoting ginseng adventitious root to occur according to claim 1, it is characterised in that: the nutrition culture
Step are as follows: nutrient medium is slowly added into bioreactor by optimization culture to after 10-15 days, continues culture 20-30 days
Adventitious root can be harvested.
8. a kind of method for promoting ginseng adventitious root to occur according to claim 7, it is characterised in that: the nutrition culture
Half-light ventilation culture at a temperature of the condition of culture of step is 23-26 DEG C.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111793636A (en) * | 2020-07-29 | 2020-10-20 | 山东农业大学 | Apple gene MdBT2 for regulating and controlling adventitious root development and application thereof |
| CN112956415A (en) * | 2021-01-18 | 2021-06-15 | 山东安然纳米实业发展有限公司 | Liquid culture medium for culturing ginseng adventitious roots and culture method |
| CN113647325A (en) * | 2021-01-18 | 2021-11-16 | 山东安然纳米实业发展有限公司 | Liquid culture medium for culturing ginseng adventitious roots and culture method |
| CN114403005A (en) * | 2022-01-27 | 2022-04-29 | 上海瑞帝安生物科技有限公司 | Ginseng adventitious root induction method and application of extract in cosmetics |
| WO2022206423A1 (en) * | 2021-04-02 | 2022-10-06 | 山东安然纳米实业发展有限公司 | Biological reaction device and culture method for adventitious roots of panax ginseng c. a. mey. |
-
2018
- 2018-03-09 CN CN201810192824.1A patent/CN110476804A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111793636A (en) * | 2020-07-29 | 2020-10-20 | 山东农业大学 | Apple gene MdBT2 for regulating and controlling adventitious root development and application thereof |
| CN112956415A (en) * | 2021-01-18 | 2021-06-15 | 山东安然纳米实业发展有限公司 | Liquid culture medium for culturing ginseng adventitious roots and culture method |
| CN112956415B (en) * | 2021-01-18 | 2021-09-24 | 山东安然纳米实业发展有限公司 | Liquid culture medium for culturing ginseng adventitious roots and culture method |
| CN113647325A (en) * | 2021-01-18 | 2021-11-16 | 山东安然纳米实业发展有限公司 | Liquid culture medium for culturing ginseng adventitious roots and culture method |
| CN113647325B (en) * | 2021-01-18 | 2022-11-29 | 山东安然纳米实业发展有限公司 | Liquid culture medium for culturing ginseng adventitious roots and culture method |
| WO2022206423A1 (en) * | 2021-04-02 | 2022-10-06 | 山东安然纳米实业发展有限公司 | Biological reaction device and culture method for adventitious roots of panax ginseng c. a. mey. |
| CN114403005A (en) * | 2022-01-27 | 2022-04-29 | 上海瑞帝安生物科技有限公司 | Ginseng adventitious root induction method and application of extract in cosmetics |
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