CN110468217A - SNP marker relevant to pig muscle pH and drip loss character and its application - Google Patents
SNP marker relevant to pig muscle pH and drip loss character and its application Download PDFInfo
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Abstract
The invention belongs to the molecular labeling preparation technical fields of pig, it is related to one kind SNP marker relevant to pig muscle pH and drip loss character and its application, the nucleotide sequence of SNP marker is to clone to obtain a special DNA fragmentation from pig KLF5 gene Second Exon area, the total 528bp of the DNA fragmentation, wherein being in the sequence A/G mutation at 160bp and at 436bp.The present invention clones to obtain a special DNA fragmentation from pig KLF5 gene extron sub-district, finds SNP site, and establish corresponding detection method, provides a kind of new molecular labeling for the Meat Quality breeding of pig.
Description
Technical field
The invention belongs to the molecular labeling preparation technical fields of pig, are related to one kind and pig muscle pH and drip loss character phase
The mononucleotide polymorphic site of the SNP marker of pass and its application more particularly to a boar KLF5 gene is as pig pH and drop
The relevant molecular labeling of water loss character and application.
Background technique
Since reform and opening-up, China's live pig breeding work achieves prominent achievement, and the speed of growth of market pig is persistently accelerated,
Carcass lean meat percentage is continuously improved.But consequent is that meat taste is coarse, meat quality decline.With people's living standard
It improves and the transformation of nutrition idea, the Pork Market in China develops to choice meat demand.Therefore, it selects preferable high-quality
Pork pig is the important topic put in face of breeding scientist.
Pig flesh characters are influenced by one or more quantitative trait locus, are difficult to be selected using conventional breeding methods
It selects.With the development of molecular biology technology, protein labeling, DNA fingerprint label, microsatellite marker, single nucleotide polymorphism
Technologies such as (single nucleotide polymorphism, SNP) are more and more applied in pig breeding actual production.
SNP is primarily referred to as the genetic marker formed in the genome due to the variation of single nucleotide acid, and quantity is more, and polymorphism is rich
Richness, PCR-RFLP are to predominantly detect one of method.These technologies reduce manpower and material resources, improve in conjunction with traditional breeding techniques
Breeding efficiency accelerates breeding process.
Kr ü pple like factor (Kr ü pple-like factors, KLFs) be a kind of zinc fingers containing C2H2 transcription because
Sub-family, KLF family member cause the function of KLFs different in the difference of the amino acid sequence of the non-bond area DNA, make
Growth, proliferation and the differentiation of cell are participated in for transcription factor, and then participate in the development and differentiation of Various Tissues organ.Study table
Bright, KLF5 passes through the expression of the regulation numerous target genes in downstream, to participate in the proliferation of vascular smooth muscle cells.However KLF5 is in bone
Regulatory mechanism in bone flesh has not been reported.
The indexs such as pH value, the drip loss of pork and meat quality are closely related, therefore related to the pork quality traits such as pH value
Gene can be used as improve Meat Quality candidate gene.
Summary of the invention
In order to solve the above technical problems in background technology, the present invention is cloned from pig KLF5 gene extron sub-district
The DNA fragmentation special to one finds SNP site, and establishes corresponding detection method, provides one for the Meat Quality breeding of pig
The new molecular labeling of kind.
A kind of SNP marker relevant to pig muscle pH and drip loss character, the nucleosides of the SNP marker
Acid sequence is to clone to obtain a special DNA fragmentation from pig KLF5 gene extron sub-district, the total 528bp of the DNA fragmentation, wherein
It is A/G mutation at 160bp and at 436bp in the sequence.
The primer pair of above-mentioned molecular labeling is expanded, the primer pair is:
F:CGTAACCCACATCAAGACC；
R:GCAATCGTAGCAGCATAG.
Application of the above-mentioned molecular labeling in breeding high quality pork.
Application of the above-mentioned molecular labeling in pig muscle pH and drip loss content detection.
Application of the above-mentioned primer pair in breeding high quality pork.
Application of the above-mentioned primer pair in pig muscle pH and drip loss content detection.
The invention has the advantages that
The present invention provides a kind of SNP marker relevant to pig muscle pH and drip loss character and its application,
The nucleotide sequence of SNP marker is to clone to obtain a special DNA fragmentation, the DNA from pig KLF5 gene extron sub-district
The total 528bp of segment, wherein being in the sequence A/G mutation at 160bp and at 436bp.Method of the invention is being protected
The exon sequence of design primer amplification pig genomic DNA is kept, includes A/G mutation at two in the segment of acquisition, and dash forward at two
Become while carrying out.And utilize the polymorphic detection pig important economical trait-of Tsp45I restriction enzyme site caused by mutational site at one
Meat Quality.The experiment proves that the mutational site and pig flesh characters there are significant related, show that the present invention utilizes the mutation position
The method of the Meat Quality of point detection pig is very accurate easy method.Method of the invention can be used for detecting the pH and drop of pig
Water loss content etc. reflects the character of meat quality, so that the molecular breeding for pig provides new detection its inhereditary feature
Method, and will play a significant role in the breeding of pig.The present invention finds KLF5 purpose using simple, fast sequencing approach
The mutational site SNPs present in genetic fragment, passes through the comparison of design of primers, PCR amplification, sequencing fragment and data result
Mutational site present on target fragment is found in analysis, and with the pork quality traits such as the pH value of pig, drip loss intramuscular fat into
Row association analysis provides a kind of new molecular labeling for high quality pork breeding.
Detailed description of the invention
Fig. 1 is that the pcr amplification product of KLF5 gene target fragment in the present invention is detected through 1.0% agarose gel electrophoresis
Result figure: in figure: M:100bp DNALadder Marker, swimming lane 1-4: amplified fragments；
Fig. 2 is Yorkshire Pigs, Berkshire, Shaziling pig, BaMa miniature pig and Daweizi pig KLF5 gene target fragment sequence
Column comparing result, the English alphabet of overstriking indicates SNP site in figure；
Fig. 3 is the Tsp45I restriction enzyme digestion and electrophoresis figure of KLF5 gene mutation site in the present invention: in figure: M:100bp DNA
Ladder Marker, swimming lane 1:AA-GG genotype, swimming lane 2,3:AG-AG genotype, swimming lane 4:GG-AA genotype.
Fig. 4 is the Sequencing chromatogram that mutational site at KLF5 gene target fragment 160bp is detected in the present invention；
Fig. 5 is the Sequencing chromatogram that mutational site at KLF5 gene target fragment 436bp is detected in the present invention.
Specific implementation method
Combined with specific embodiments below to the present invention be further explained, but be embodied the present invention is not done it is any
It limits.
The present invention provides a kind of SNP marker relevant to pig muscle pH and drip loss character, SNP molecule marks
The nucleotide sequence of note is to clone to obtain a special DNA fragmentation from pig KLF5 gene extron sub-district, and the DNA fragmentation is total
528bp, wherein being in the sequence A/G mutation at 160bp and at 436bp.
It is present on detection pig KLF5 gene 528bp amplified fragments meanwhile the present invention also provides a kind of detection method
Two base mutation sites, at one at target fragment 160bp, another place is A/G mutation at target fragment 436bp, and
There are correlations for mutation at two, and the genotype of pig is determined by base, then determines Meat Quality by genotype.Determine pig
The method of genotype are as follows: when base is A at 160bp, base is G at 436bp；When base is G at 160bp, in 436bp
Place's base is A.Base changes simultaneously at two, and there are three types, respectively AA-GG, AG-AG, GG-AA.Testing goal segment
Base is A or G to determine genotype classes at 160bp and 436bp, and method includes the amplification that PCR amplification obtains target fragment
Product, to amplified production carry out be sequenced or with Tsp45I enzyme (436bp base be G when nearby existing for a kind of restriction enzyme)
Cut amplified production.Tsp45I digestion amplified fragments, if obtaining two segments of 92bp, 436bp, genotype AA-GG, if obtaining
Mono- segment of 528bp, genotype GG-AA；If obtaining tri- segments of 92bp, 436bp and 528bp, genotype AG-
AG。
In order to keep method easier, have higher efficiency, the detection method that segment is obtained after above-mentioned digestion is agarose
Detected through gel electrophoresis.
1, the foundation of the method for RFLP polymorphic detection pig flesh characters
In the gene mutation site design primer, SNP parting, primer sequence such as table are carried out using Tsp45I-RFLP method
1。
1 primer sequence of table
DNA sample for genetic polymorphism detection comes from 5 swinerys, is shown in Table 2.Genomic DNA is extracted using kit,
It is placed in -20 DEG C of preservations.
2 swinery of table and sample number
PCR amplification condition:
20 μ L:DNA template of PCR amplification system 1 μ L, each 0.5 μ L of upstream and downstream primer, 8 μ L, 2 × Taq PCR of distilled water
Master Mix10μL；PCR amplification program: 94 DEG C of initial denaturation 5min, 94 DEG C of denaturation 30s, 58 DEG C of annealing 30s, 72 DEG C extend
30s, 2-4 walk 34 circulations, extend 5min, 4 DEG C of preservations after 72 DEG C；Pcr amplification product is examined through 1.0% agarose gel electrophoresis
It surveys, the result is shown in Figure 1.
Target fragment sequencing:
The target fragment that previous step is expanded send company to be sequenced, and obtains the sequence of target fragment.Yorkshire Pigs, Bark
The difference pig kind KLF5 gene target fragment sequence comparison such as summer pig, Shaziling pig, BaMa miniature pig and Daweizi pig sees Fig. 2,
Wherein, the English alphabet of overstriking indicates SNP site in figure.
Tsp45I-RFLP detection:
PCR product Tsp45I digestion, endonuclease reaction volume are 31 μ L, wherein 10 μ L, nuclease- of pcr amplification product
18 μ L, 10 × Buffer R of free water 2 μ L, 1 μ L (10U) of restriction enzyme will be centrifuged, 37 DEG C of temperature after sample blending
Bathe 16h.
Digestion is detected with 2% agarose gel electrophoresis as a result, record genotype, gel imaging system photograph, are as a result shown in figure
3。
The result shows that obtaining the DNA fragmentation that length is 528bp using primer pair KLF5 gene magnification.By sequencing, in mesh
Segment 160bp and 436bp at base be A or G mutational site, it is A according to the place 436bp base that Sequencing chromatogram, which is shown in Fig. 4, Fig. 5,
Or G finds suitable restriction enzyme Tsp45I, then carries out Tsp45I-RFLP and detects polymorphic site.
Using pig KLF5 gene as research object, using 5 swinerys (270 total) as subjects, acquires ear tissue and mention
Take DNA, wherein Yorkshire Pigs 78, Berkshire 62, Shaziling pig 45, BaMa miniature pig 30, Daweizi pig 55.
Gene frequency, the genotype frequency in the site A/G at KLF5 gene 436bp are detected, and analyze its genetic structure.
Tsp45I-RFLP detection polymorphic site the results are shown in Table 3.As shown in Table 3, in 5 pig kinds detected, AA-GG
Genotype and A-G allele have comparative advantage, but GG-AA type is temporarily not detected in Shaziling pig.
Distribution of the 3 Tsp45I-RFLP polymorphism of table in 5 swinerys
2, the association analysis of genotype and Meat Quality
Test swinery: selection three way cross kind, i.e. Large White × Berkshire × Shaziling pig (abbreviation bus is husky), it is young
After pig wean, select the piglet that 200 dates of birth are close, growth is more consistent as test swinery.When test pig groups weight
Reach 100kg or so, butchered, and detect genotype according to the method for step 1), detects Meat Quality by the following method.
Meat Quality detection method:
PH: 45 minutes (pH after slaughter1) and 24 hours (pH24) combined respectively with acidometer measurement longissimus dorsi muscle Thoracolumbar disk
Locate pH value.
Drip loss: longissimus dorsi muscle at inverted 3-4 thoracic vertebrae in 2 hours after butchering removes fascia, adipose tissue, suitable
The meat sample of 2cm × 2cm × 4cm (height × width x length) is cut into muscle fibre direction, is placed on electronic balance and is weighed, and hooks meat with tied silk
Sample one end, is hung on muscle fibre in disposal plastic cup vertically downward, is placed in the polyethylene food bag of inflation, tightens bag
Mouthful, avoid meat sample from contacting with wall of cup.It is stored 48 hours under the conditions of 2-4 DEG C, takes out meat sample, gently blot meat sample surface layer with filter paper
Residual liquid is placed on electronic balance and weighs, and the difference of front and back weight is drip loss divided by the percentage of fresh meat weight.
The GLM program of different genotype and 9.4 version of association analysis application SAS of pig flesh characters (pH, drip loss)
Data processing and statistical analysis are carried out, using model are as follows: Yijk=μ+Bi+Gj+ ε ijk.Wherein, Yijk is trait phenotypes value, μ
For average value, Bi is combined effect, and Gj is genotype effects, and ε ijk is random error.
As the result is shown: the pH1 and pH of AA-GG genotype24It is significantly higher than GG-AA type (P < 0.05), but AA-GG and GG-AA
Type is not significant (P > 0.05) with AG-AG type difference；The drip loss of AA-GG genotype is significantly higher than AG-AG and GG-AA type (P
<0.05).(present invention has also statisticallyd analyze other Meat Quality indexs, only lists significant difference index).
The genotype of 4 pig of table and the association analysis of Meat Quality
With column data shoulder mark, different letters indicate significant difference (P < 0.05)；Shoulder mark same letter is indicated without letter
Difference is not significant (P > 0.05).
Sequence table
<110>Hunan Prov. Research Inst. of Animal Husbandry and Veterinary
<120>SNP marker relevant to pig muscle pH and drip loss character and its application
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
cgtaacccac atcaagacc 19
<210> 2
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
gcaatcgtag cagcatag 18
Claims (6)
1. a kind of SNP marker relevant to pig muscle pH and drip loss character, it is characterised in that: the SNP molecule
The nucleotide sequence of label is to clone to obtain a special DNA fragmentation from pig KLF5 gene extron sub-district, and the DNA fragmentation is total
528bp, wherein being in the sequence A/G mutation at 160bp and at 436bp.
2. expanding the primer pair of molecular labeling as described in claim 1, it is characterised in that: the primer pair is:
F:CGTAACCCACATCAAGACC；
R:GCAATCGTAGCAGCATAG.
3. application of the molecular labeling according to claim 1 in breeding high quality pork.
4. application of the molecular labeling according to claim 3 in pig muscle pH and drip loss content detection.
5. application of the primer pair according to claim 2 in breeding high quality pork.
6. application of the primer pair according to claim 5 in pig muscle pH and drip loss content detection.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113718041A (en) * | 2021-08-30 | 2021-11-30 | 福建省农业科学院畜牧兽医研究所 | SNP molecular marker related to meat rabbit muscle drip loss character and application thereof |
| CN114045350A (en) * | 2021-12-14 | 2022-02-15 | 华中农业大学 | SNP molecular marker associated with porcine muscle drip loss character and application thereof |
| CN114350821A (en) * | 2022-01-20 | 2022-04-15 | 湖北省农业科学院畜牧兽医研究所 | Molecular marker related to pH value and lean meat percentage of pig muscle and application thereof |
| CN118086522A (en) * | 2024-03-07 | 2024-05-28 | 北京顺双龙牧业有限公司 | Molecular marker related to backfat thickness of pigs and drip loss character of pork |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911377A (en) * | 2014-04-28 | 2014-07-09 | 华中农业大学 | Molecular marker identification relevant to pH value character of pork and applications thereof |
| CN104372006A (en) * | 2014-10-24 | 2015-02-25 | 四川农业大学 | Molecular marker related to pig muscle pH value character and application thereof |
| CN104480216A (en) * | 2014-12-31 | 2015-04-01 | 华中农业大学 | Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle |
| CN106906303A (en) * | 2017-04-27 | 2017-06-30 | 江西农业大学 | One SNP marker for influenceing quality character of pork and its application |
| CN107937552A (en) * | 2017-08-01 | 2018-04-20 | 南京农业大学 | A kind of and the relevant SNP marker of Suhuai pig color traits and its primer and application |
| CN109576276A (en) * | 2018-12-18 | 2019-04-05 | 南京农业大学 | The molecular cloning of pork quality trait related gene Prox1 and application |
-
2019
- 2019-09-11 CN CN201910858804.8A patent/CN110468217B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911377A (en) * | 2014-04-28 | 2014-07-09 | 华中农业大学 | Molecular marker identification relevant to pH value character of pork and applications thereof |
| CN104372006A (en) * | 2014-10-24 | 2015-02-25 | 四川农业大学 | Molecular marker related to pig muscle pH value character and application thereof |
| CN104480216A (en) * | 2014-12-31 | 2015-04-01 | 华中农业大学 | Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle |
| CN106906303A (en) * | 2017-04-27 | 2017-06-30 | 江西农业大学 | One SNP marker for influenceing quality character of pork and its application |
| CN107937552A (en) * | 2017-08-01 | 2018-04-20 | 南京农业大学 | A kind of and the relevant SNP marker of Suhuai pig color traits and its primer and application |
| CN109576276A (en) * | 2018-12-18 | 2019-04-05 | 南京农业大学 | The molecular cloning of pork quality trait related gene Prox1 and application |
Non-Patent Citations (2)
| Title |
|---|
| ENSEMBL RELEASE 97: "Ensembl Pig（scrofa11.1）", 《EMBL-EBI》 * |
| YUDING WANG等: "Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs", 《BMC GENOMICS》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113718041A (en) * | 2021-08-30 | 2021-11-30 | 福建省农业科学院畜牧兽医研究所 | SNP molecular marker related to meat rabbit muscle drip loss character and application thereof |
| CN113718041B (en) * | 2021-08-30 | 2023-06-30 | 福建省农业科学院畜牧兽医研究所 | SNP molecular marker related to muscle drip loss character of meat rabbits and application thereof |
| CN114045350A (en) * | 2021-12-14 | 2022-02-15 | 华中农业大学 | SNP molecular marker associated with porcine muscle drip loss character and application thereof |
| CN114350821A (en) * | 2022-01-20 | 2022-04-15 | 湖北省农业科学院畜牧兽医研究所 | Molecular marker related to pH value and lean meat percentage of pig muscle and application thereof |
| CN114350821B (en) * | 2022-01-20 | 2023-10-03 | 湖北省农业科学院畜牧兽医研究所 | Molecular marker related to pig muscle pH value and lean meat percentage and application thereof |
| CN118086522A (en) * | 2024-03-07 | 2024-05-28 | 北京顺双龙牧业有限公司 | Molecular marker related to backfat thickness of pigs and drip loss character of pork |
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