CN110468051A - A kind of K252A fermentation medium and preparation method thereof - Google Patents

A kind of K252A fermentation medium and preparation method thereof Download PDF

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CN110468051A
CN110468051A CN201910698830.9A CN201910698830A CN110468051A CN 110468051 A CN110468051 A CN 110468051A CN 201910698830 A CN201910698830 A CN 201910698830A CN 110468051 A CN110468051 A CN 110468051A
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彭湘屏
朱进伟
张敏
石磊
高祥
聂玲燕
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ZHEJIANG HAIZHENG PHARMACEUTICAL CO Ltd
Haizheng Pharmaceutical (hangzhou) Co Ltd
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Haizheng Pharmaceutical (hangzhou) Co Ltd
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
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Abstract

The invention discloses a kind of K252A fermentation mediums and preparation method thereof, the component collocation that the fermentation medium passes through optimization, the function factors such as additional choline chloride, disodium succinate, methionine, it can be grown for Norcardiopsis, metabolism provides nutrition, intermediate can be provided for K252A biosynthesis again after metabolic conversion, the final fermentation liquid for obtaining high yield K252A.Using fermentation medium of the present invention, shake flask fermentation 9 days, K252A potency was higher than 2.60g/L.By controlling two pH, dissolved oxygen critical crafts, while optimizing the control of additive raw material strategy of glucose and function factor, cultivates to about 264h, K252A potency is significantly improved up to 5.79g/L, level more reported in the literature, has the basis of industrialized production.

Description

A kind of K252A fermentation medium and preparation method thereof
Technical field
The present invention relates to industrial microorganism fermentation technical fields, and in particular to a kind of K252A fermentation medium and by the hair The method that ferment culture medium prepares K252A.
Background technique
K252A, CAS 99533-80-9, be 1986 by HIROSHI KASE et al. from nocardia culture Isolated metabolite, completes Structural Identification later, and final confirmation is the analog of staurosporin, is a kind of potent The inhibitor of protein kinase C and tyrosine kinase, IC50Value is 32.9nM.Currently, lot of documents reports antibiotic K252A tool Standby effective anti-tumor activity, but researches show that the toxicity in vivo for not having antimicrobial acivity or rodent in vitro. It is a kind of effective Ca that widely research, which is proved antibiotic K252A,2+/ cam kinase II inhibitor, it can also inhibit Other kinase activities, especially myosin light chain kinase, cyclic adenosine monophosphate dependent kinases (PKA), protein kinase C (PKC) and cGMP rely on protein kinase (PKG).
Known Norcardiopsis (Nocardiopsis sp.) can produce K252A, and HIROSHI KASE was in 1986 Carried out outside brief report, but in its obtained fermentation liquid K252A content be only about 110 μ g/ml (Hiroshi Kase, Kazuyuki Iwahashi,Yuzuru Matsuda.K-252a,a Potent Inhibitor of Protein Kinase C from Microbial Origin[J].The Journal of Antibiotics,1986,39(8):1059-1065.)。 Mitsutaka Kino once reported that the fermentation level of its K252A in the fermenter reached 2g/L, but so far there are no that it has subsequent text Offer disclosure (Mitsutaka Kino, Kenzo Shono, Tetsuo Nishinura, et al.Practical Preparation of K-252a from a Fermentation Solution[J] .Biosci.Biotechnol.Biochem.,1998,62(8):1627-1629.).Sanjay R.Chemburkar etc. is special It reports that its K252A fermentation level is 1~4g/L in sharp US20090239271A1, and the separation method of K252A is protected Shield, but its undisclosed specific fermentative medium formula and fermentation culture method.
Summary of the invention
In order to improve the yield of K252A, the present invention is using Norcardiopsis (Nocardiopsis sp.) as production bacterium Kind, research optimization is carried out to fermentation medium and fermentation process, finally obtains a kind of fermentation medium and by the fermentation medium The method for preparing K252A.While the culture medium and method provide sufficient nutrition for Norcardiopsis growth, it can promote quasi- The accretion rate of Nocard's bacillus kind provides intermediate by the metabolic conversion of function factor for the biosynthesis of K252A, most The fermentation yield of K252A is significantly improved eventually.
A kind of fermentation medium of K252A, the fermentation medium include the component of following weight percentage:
Wherein, the function factor are as follows:
The combination of choline chloride, disodium succinate, methionine three, wherein choline chloride content is fermentation medium weight 0.01~0.05%, disodium succinate content be fermentation medium weight 0.08~0.4%, methionine content be fermentation training Support the 0.26~1.3% of base weight amount.
Preferably, the carbon source be one of the following or two or more of combinations: glycerol, dextrin, soluble starch, Maltose, methyl oleate.The carbon source includes quick-acting, slow carbon source, is mainly used for different times and provides for thalli growth, breeding Carbon component needed for required energy and synthesis thallus, stimulation mycelia grows, while providing carbon skeleton for Product formation.
As further preferred, wherein the carbon source is preferably methyl oleate or the preferably combination containing methyl oleate. Methyl oleate has multi-efficiency, in sterilisation stage, is easy blistering top tank, and methyl oleate, therefore can be with due to defoaming effect The dosage for reducing substrate defoaming agent, to reduce defoaming agent to the inhibiting effect of thalli growth；In cultivation stage, both can be used as Basic carbon source is slowly utilized by thallus, and can accelerate product out of cell to extracellular diffusion, to reduce feedback inhibition effect It answers.
Preferably, the nitrogen source is one of the following or two or more of combinations: soybean cake powder, cottonseed meal, jade Rice flour, yeast extract, Dried Corn Steep Liquor Powder.The nitrogen source includes quick-acting, slow nitrogen source, is mainly used for different times building thallus The nitrogen skeleton of the cellular materials such as amino acid, protein and nucleic acid and Product formation.
Preferably, the inorganic salts are one of the following or two or more of combinations: ammonium salt, calcium salt, magnesium salts, potassium The combination of salt, sodium salt, phosphate, sulfate, nitrate, preferably ammonium nitrate, magnesium sulfate, dipotassium hydrogen phosphate, calcium carbonate.It is described Inorganic salts may be constructed somatic cells ingredient, while the important component as microbial enzyme, can also be used as certain enzymes Activator or inhibitor are to adjust the approach and efficiency of Product formation.The inorganic salts can also pH value to culture medium into Row buffering is adjusted, and constructs buffer system appropriate, it is also possible to be maintained osmotic pressure appropriate and oxidation-reduction potential etc., be participated in life The electron transmission of object metabolic process.
Preferably, the defoaming agent is THIX-298 defoaming agent.
As further preferred, the fermentation medium includes the component of following weight percentage:
PH value is 6.4~8.0 before the fermentation medium sterilizes.
Fermentation medium of the present invention not only contains carbon source, also contains the components such as nitrogen source, inorganic salts, function factor, Sufficient nutrition can either be provided for Norcardiopsis (Nocardiopsis sp.), and the generation of Norcardiopsis can be promoted It thanks to speed, provides intermediate for K252A biosynthesis, finally greatly improve the fermentation yield of K252A.
The present invention also provides the methods that fermentation medium described in a kind of application prepares K252A comprising following steps:
Shake flask fermentation method: shake-flask seed culture solution is seeded to above-mentioned fermentation medium according to 5~15% volume ratio In, it is cultivated under the conditions of 26~32 DEG C, 150~500rpm, 220~240h of cultivation cycle obtains the fermentation liquid containing K252A；
Or
Tank fermentation process: tank seed culture fluid is seeded to above-mentioned hair according to the ratio of fermentation medium volume 5~10% In ferment culture medium, cultivated under the conditions of 26~32 DEG C, by stirring and ventilation linkage control, make dissolved oxygen maintain 15 always~ 30%.Inoculation starts to culture to terminate for 24 hours afterwards, and carrying out pH with ammonium hydroxide makes its control 6.8 ± 0.2；Start after inoculation 36h, the phase Between stream plus glucose solution and function factor mixed liquor, until culture terminate stoppings in first 1 day stream add, 220~264h of cultivation cycle is obtained To the fermentation liquid containing K252A.
Preferably, the control strategy of stream plus glucose solution is to maintain glucose in fermentation liquid dense in the tank fermentation process For degree within the scope of 1~5g/L, the concentration for the glucose solution that the stream adds is 500g/L.
Preferably, the function factor mixed liquor ingredient added is flowed in the tank fermentation process are as follows: choline chloride 5g/L, succinic acid Disodium 40g/L, methionine 130g/L, surplus are water, and the speed control of the stream plus function factor mixed liquor adds in stream per hour Volume is the 0.05~0.1% of preliminary fermentation culture medium volume.
Preferably, the shake-flask seed culture solution is obtained by following step:
1) it screens Norcardiopsis: using solid plate culture medium, select the big bacterium colony of Starch Hydrolysis circle；
2) effective inoculation source: the excellent bacterium colony of picking is prepared, is inoculated in solid slope culture medium, at 26~32 DEG C, 55 Stationary culture 5 days under~65% relative humidity collect fresh, mature lawn and are used as effective inoculation source；
3) shake-flask seed culture: the provenance of step 2) is seeded in seed culture medium, at 26~32 DEG C, 150~ 24~72h is cultivated under the conditions of 330rpm, obtains shake-flask seed culture solution；
The tank seed culture fluid is obtained by following step: shake-flask seed culture solution is first inoculated into seeding tank In seed culture medium, expand 18~96h of culture under the conditions of 26~32 DEG C, 50~300rpm, 0.35vvm, obtains the training of tank seed Nutrient solution.
It is further preferred that solid slope culture medium packet described in solid plate culture medium described in step 1) and step 2) Include the component of following weight percentage:
PH value is 6.5~7.5 before the solid medium sterilizes, and cornstarch is used to carry out strain for carbon source in step 1) Screening, can intuitively judge the metabolic capability of strain by bacterium colony to the size of Starch Hydrolysis circle, eliminate metabolic capability phase To colony diameter >=5mm that poor strain, the culture medium are cultivated, bacterium colony is towering, and lawn is plentiful.
Preferably, seed culture medium described in step 3) includes the component of following weight percentage:
PH value is 6.5~7.5 before the seed culture medium sterilizes, which can provide stimulation mycelia and grow, Sufficient carbon nitrogen source nutrition is provided, mycelia is made to dissipate, is sturdy, flush and be not easy aging.
Using fermentation medium of the present invention and its method for preparing K252A, shake flask fermentation 9 days, K252A potency was higher than 2.60g/L is free from 5.26 times of the fermentation medium of function factor.Pass through fermentor process conditions and control of additive raw material, culture To about 264h, K252A potency reaches as high as 5.79g/L, and relatively level reported in the literature significantly improves at present.
Compared with prior art, the present invention has the following advantage:
1, fermentation medium provided by the invention can grow for Norcardiopsis, metabolism provides nutrition, pass through chlorination The addition of choline, disodium succinate, the methionine combination function factor, K252A potency are higher than 2.6g/L.
2, glucose and function are optimized by control two pH, dissolved oxygen critical crafts in tank fermented and cultured of the present invention The control of additive raw material strategy of the factor, the content of K252A further greatly improves in fermentation liquid, reaches 5.7g/L or more, compared with document report Road level significantly improves.
3, K252A fermentation medium of the present invention and preparation method thereof is tested through the progress technique amplification of 500L scale fermentation tank It demonstrate,proves, the content of K252A can achieve 5.7g/L or more in fermentation liquid, have the basis of industrialized production.
Specific embodiment
In order to which technology contents, the construction feature, the objects and the effects of technical solution is described in detail, below in conjunction with specific Case study on implementation is described in detail.Obviously, described embodiments are only a part of the embodiments of the present invention, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts The every other embodiment obtained, shall fall within the protection scope of the present invention.
Nutrient media components used in following example are as follows:
Solid plate culture medium composition: cornstarch 1%, caseinhydrolysate 0.2%, beef extract 0.2%, yeast extract 0.2%, agar 2%, surplus is water, and pH value is 6.5~7.5 before the culture medium sterilizes.
Solid slope culture medium composition: cornstarch 1%, caseinhydrolysate 0.2%, beef extract 0.2%, yeast extract 0.2%, agar 2%, surplus is water, and pH value is 6.5~7.5 before the culture medium sterilizes.
Seed culture medium composition: glycerol 0.5%, cornstarch 3%, soybean cake powder 2%, yeast extract 0.5%, corn Paste dry powder 0.5%, calcium carbonate 0.3%, surplus are water, and pH value is 6.5~7.5 before the seed culture medium sterilizes.
Strain source in the present invention: Culture Collection Center, Nocardiopsis sp.K- are studied purchased from american agriculture 252, NRRL 15532.
Comparative example 1:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under 60% relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 72h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) shake flask fermentation culture: the seed culture fluid of step 3) is seeded to according to 5% volume ratio without methyl oleate In the fermentation medium of function factor, culture obtained the fermentation liquid containing K252A to 9 days under the conditions of 28 DEG C, 200rpm；
Fermentative medium formula (being free of function factor) composition are as follows: glycerol 0.4%, maltose 1%, maltodextrin 9% can Soluble starch 4%, soybean cake powder 0.2%, corn flour 1%, cottonseed meal 1%, yeast extract 3%, Dried Corn Steep Liquor Powder 0.5%, ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, calcium carbonate 0.1%, surplus is water, and culture medium disappears PH value before poison is 6.5.
5) sample treatment and detection: taking fermentation liquid 1ml, 5ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, from Supernatant HPLC analysis measurement is taken after the heart or filtering, the content 0.504g/L of K252A in fermentation liquid.
Embodiment 1:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under 60% relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 72h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) seed culture fluid of step 3) shake flask fermentation culture: is seeded to addition function factor according to 5% volume ratio Without in methyl oleate fermentation medium, culture obtained the fermentation containing K252A to 9 days under the conditions of 28 DEG C, 200rpm Liquid；
Fermentative medium formula (containing function factor) composition are as follows: glycerol 0.4%, maltose 1%, maltodextrin 9% are solvable Property starch 4%, soybean cake powder 0.2%, corn flour 1%, cottonseed meal 1%, yeast extract 3%, Dried Corn Steep Liquor Powder 0.5%, Ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, calcium carbonate 0.1%, choline chloride 0.05%, succinic acid two Sodium 0.4%, methionine 1.3%, surplus are water, and the pH value before culture medium disinfection is 6.5.
5) sample treatment and detection: taking fermentation liquid 1ml, 5ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, from Supernatant HPLC analysis measurement is taken after the heart or filtering, the content 2.651g/L of K252A in fermentation liquid.
Embodiment 2:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under 60% relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 72h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) shake flask fermentation culture: the seed culture fluid of step 3) is seeded to according to 5% volume ratio containing methyl oleate And in the fermentation medium of addition function factor, culture obtained the fermentation containing K252A to 9 days under the conditions of 28 DEG C, 200rpm Liquid；
Fermentative medium formula (containing function factor and methyl oleate) composition are as follows: methyl oleate 1.5%, glycerol 0.4%, wheat Bud sugar 1%, maltodextrin 9%, soluble starch 4%, soybean cake powder 0.2%, corn flour 1%, cottonseed meal 1%, yeast are taken out Extract 3%, Dried Corn Steep Liquor Powder 0.5%, ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, calcium carbonate 0.1%, Choline chloride 0.05%, disodium succinate 0.4%, methionine 1.3%, surplus are water, and the pH value before culture medium disinfection is 6.5.
5) sample treatment and detection: taking fermentation liquid 1ml, 5ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, from Supernatant HPLC analysis measurement is taken after the heart or filtering, the content 2.773g/L of K252A in fermentation liquid.
Comparative example 2:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under 60% relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 68h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) tank fermented and cultured: the shake-flask seed culture solution of step 3) is seeded in the ratio of culture volume 0.25% In 10L seed culture medium, expands culture under the conditions of 29 DEG C, 150rpm, 0.35vvm to 48h, obtain tank seed culture fluid；Again Tank seed culture fluid is seeded in the fermentation medium without methyl oleate according to the ratio of fermentation medium volume 8%, In 28 DEG C, 150~500rpm, cultivate under the conditions of 0.35vvm, controlled without pH and dissolved oxygen, no-feed supplement, cultivate to 256h, obtain Fermentation liquid containing K252A；
Fermentative medium formula (containing function factor) composition are as follows: glycerol 0.4%, maltose 1%, maltodextrin 5% are solvable Property starch 3%, soybean cake powder 0.6%, corn flour 1.5%, cottonseed meal 1.5%, yeast extract 2.5%, Dried Corn Steep Liquor Powder 0.75%, ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, calcium carbonate 0.1%, choline chloride 0.05%, fourth Two acid disodiums 0.4%, methionine 1.3%, surplus are water, and the pH value before culture medium disinfection is 6.5.
5) sample treatment and detection: taking fermentation liquid 1ml, 5ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, from Supernatant HPLC analysis measurement is taken after the heart or filtering, the content 2.746g/L of K252A in fermentation liquid.
Comparative example 3:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, 60% Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 68h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) tank fermented and cultured: the shake-flask seed culture solution of step 3) is seeded in the ratio of culture volume 0.25% In 10L seed culture medium, expands culture under the conditions of 29 DEG C, 150rpm, 0.35vvm to 48h, obtain tank seed culture fluid；Again Tank seed culture fluid is seeded to the fermentation medium comprising 1.5% methyl oleate according to the ratio of fermentation medium volume 8% In, it cultivates, is controlled without pH and dissolved oxygen, no-feed supplement under the conditions of 28 DEG C, 150~500rpm, 0.35vvm, culture to 260h, Obtain the fermentation liquid containing K252A；
Fermentative medium formula (containing function factor and methyl oleate) composition are as follows: methyl oleate 1.5%, glycerol 0.4%, wheat Bud sugar 1%, maltodextrin 5%, soluble starch 3%, soybean cake powder 0.6%, corn flour 1.5%, cottonseed meal 1.5%, ferment Female extract 2.5%, Dried Corn Steep Liquor Powder 0.75%, ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, carbonic acid Calcium 0.1%, choline chloride 0.05%, disodium succinate 0.4%, methionine 1.3%, surplus are water, the pH before culture medium disinfection Value is 6.5.
5) sample treatment and detection: taking fermentation liquid 1ml, 5ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, from Supernatant HPLC analysis measurement is taken after the heart or filtering, the content 2.918g/L of K252A in fermentation liquid.
Embodiment 3:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under 60% relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 68h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) tank fermented and cultured: the shake-flask seed culture solution of step 3) is seeded in the ratio of culture volume 0.25% In 10L seed culture medium, expands culture under the conditions of 29 DEG C, 150rpm, 0.35vvm to 48h, obtain tank seed culture fluid；Again Tank seed culture fluid is seeded to according to the ratio of fermentation medium volume 8% comprising 3% methyl oleate, low concentration function factor Fermentation medium in, under the conditions of 28 DEG C, pass through stirring with ventilation linkage control, maintain dissolved oxygen 15~30%.After inoculation for 24 hours Start to terminate to culture, pH is controlled automatically in 6.8 ± 0.2 ranges with ammonium hydroxide.Start after inoculation 36h, stream plus the Portugal 500g/L Grape sugar juice and function factor mixed liquor maintain 1~5g/L of concentration of glucose in fermentation liquid, until 240h stops stream plus glucose Solution and function factor mixed liquor continue culture to 264h, obtain the fermentation liquid containing K252A；
Fermentative medium formula (containing function factor and methyl oleate) composition are as follows: glycerol 0.4%, maltose 0.5%, malt Dextrin 5%, soluble starch 3%, methyl oleate 3%, soybean cake powder 0.6%, corn flour 1.5%, cottonseed meal 1.5%, ferment Female extract 2.5%, Dried Corn Steep Liquor Powder 0.75%, ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, carbonic acid Calcium 0.1%, choline chloride 0.01%, disodium succinate 0.08%, methionine 0.26%, surplus is water, before culture medium disinfection PH value is 6.5.
Function factor mixed liquor composition are as follows: choline chloride 5g/L, disodium succinate 40g/L, methionine 130g/L, surplus are Water；
5) sample treatment and detection: taking fermentation liquid 1ml, 9ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, from Supernatant HPLC analysis measurement is taken after the heart or filtering, the content 5.702g/L of K252A in fermentation liquid.
Embodiment 4:
1) it screens Norcardiopsis: taking work strain cryopreservation tube, separate in dibbling to solid plate culture medium, select shallow lake Big bacterium colony is enclosed in powder hydrolysis；
2) prepare effective inoculation source: excellent bacterium colony 1 of picking is inoculated into solid slope culture medium, at 28 DEG C, Fresh, mature lawn is collected as effective inoculation source in stationary culture 5 days under 60% relative humidity immediately；
3) shake-flask seed culture: by the solid slope lawn about 1cm of step 2)2It is seeded in 50ml seed culture medium, In 28 DEG C, 68h is cultivated under the conditions of 200rpm, obtains shake-flask seed culture solution；
4) tank fermented and cultured: the shake-flask seed culture solution of step 3) is seeded in the ratio of culture volume 0.25% In 30L seed culture medium, culture obtains tank seed culture fluid to 52h under the conditions of 29 DEG C, 150rpm, 0.35vvm；Again by tank Seed culture fluid is seeded to the hair comprising 3% methyl oleate, low concentration function factor according to the ratio of fermentation medium volume 6% In ferment culture medium, under the conditions of 28 DEG C, by stirring and ventilation linkage control, dissolved oxygen 15~30% is maintained.Inoculation starts afterwards for 24 hours Terminate to culture, pH is controlled automatically in 6.8 ± 0.2 ranges with ammonium hydroxide, starts after being inoculated with 36h, stream plus 500g/L glucose Solution and function factor mixed liquor maintain 1~5g/L of concentration of glucose in fermentation liquid, until 240h stops stream plus glucose solution With function factor mixed liquor, continues culture to 264h, obtain the fermentation liquid containing K252A；
Fermentative medium formula (containing function factor and methyl oleate) composition are as follows: methyl oleate 3%, glycerol 0.4%, malt Sugar 0.5%, maltodextrin 5%, soluble starch 3%, soybean cake powder 0.6%, corn flour 1.5%, cottonseed meal 1.5%, ferment Female extract 2.5%, Dried Corn Steep Liquor Powder 0.75%, ammonium nitrate 0.1%, magnesium sulfate 0.5%, dipotassium hydrogen phosphate 0.02%, carbonic acid Calcium 0.1%, choline chloride 0.01%, disodium succinate 0.08%, methionine 0.26%, surplus is water, before culture medium disinfection PH value is 6.5.
Function factor mixed liquor composition are as follows: choline chloride 5g/L, disodium succinate 40g/L, methionine 130g/L, surplus are Water；
5) sample treatment and detection: taking fermentation liquid 1ml, 9ml anhydrous methanol be added, mix, and ultrasonic 1h is mixed again, is centrifuged Or supernatant HPLC analysis measurement is taken after filtering, the content 5.790g/L of K252A in fermentation liquid.
It can be seen that from the correlation data of comparative example 1 and embodiment 1 through choline chloride, disodium succinate, methionine group The addition of function factor is closed, the potency of K252A significantly improves, and reaches 2.6g/L, 5.26 not added about times.
From the comparison of embodiment 1 and embodiment 2, the comparison of comparative example 2 and comparative example 3, it can be seen that the present invention, which is fermented, to be trained It supports to add in base and adds the raising yield that methyl oleate is conducive to K252A yield in methyl oleate, especially tank fermentation medium.
It can be seen that the present invention from the correlation data of comparative example 3 and embodiment 3~4 and pass through control two pH, dissolved oxygen keys Process conditions optimize the control of additive raw material strategy of glucose and function factor mixed liquor, and the content of K252A is further big in fermentation liquid Width improves, and still can achieve 5.79g/L after enlarged experiment, significantly improves compared with document report level, have industrialized production Basis.
It should be noted that not thereby being limited although above-mentioned each case study on implementation has been described herein Make scope of patent protection of the invention.Therefore, based on innovative idea of the invention, the change that case study on implementation described herein is carried out And modification or the equivalent structure or equivalent process transformation made by using the contents of the present specification, directly or indirectly will more than Technical solution is used in other correlative technology fields, is included within scope of patent protection of the invention.

Claims (12)

1. a kind of fermentation medium of K252A, it is characterised in that: the fermentation medium includes the group of following weight percentage Point:
Wherein, the function factor are as follows:
The combination of choline chloride, disodium succinate, methionine three, wherein choline chloride content is fermentation medium weight 0.01~0.05%, disodium succinate content is the 0.08~0.4% of fermentation medium weight, and methionine content is fermented and cultured The 0.26~1.3% of base weight amount.
2. the fermentation medium of K252A according to claim 1, it is characterised in that: the carbon source is one of the following Or two or more of combinations: glycerol, dextrin, soluble starch, maltose, methyl oleate, wherein the carbon source is preferably oil Sour methyl esters or the preferably combination containing methyl oleate.
3. the fermentation medium of K252A according to claim 1, it is characterised in that: the nitrogen source is one of the following Or two or more of combinations: soybean cake powder, cottonseed meal, corn flour, yeast extract, Dried Corn Steep Liquor Powder.
4. the fermentation medium of K252A according to claim 1, it is characterised in that: the inorganic salts are following middle one kind Or two or more of combinations: ammonium salt, calcium salt, magnesium salts, sylvite, phosphate, sulfate, nitrate, preferably ammonium nitrate, sulfuric acid The combination of magnesium, dipotassium hydrogen phosphate, calcium carbonate.
5. the fermentation medium of K252A according to claim 1, it is characterised in that: the defoaming agent disappears for THIX-298 Infusion.
6. the fermentation medium of K252A according to claim 1-5, which is characterized in that the fermentation medium Component including following weight percentage:
PH value is 6.4~8.0 before the fermentation medium sterilizes.
7. the method that described in any item fermentation mediums prepare K252A according to claim 1~6, it is characterised in that: it is wrapped It includes,
Shake flask fermentation method: shake-flask seed culture solution is seeded to according to 5~15% volume ratio any in claim 1~6 In fermentation medium described in, cultivated under the conditions of 26~32 DEG C, 150~500rpm, 200~240h of cultivation cycle is obtained Fermentation liquid containing K252A；
Or
Tank fermentation process: by tank seed culture fluid according to the ratio of fermentation medium volume 5~10% be seeded to claim 1~ It in fermentation medium described in any one of 6, cultivates under the conditions of 26~32 DEG C, by stirring and ventilation linkage control, makes molten Oxygen maintains 15~30% always；Inoculation starts to culture to terminate for 24 hours afterwards, and adjusting pH with ammonium hydroxide makes its control 6.8 ± 0.2； Start after inoculation 36h, during which stream plus glucose solution and function factor mixed liquor, adds until culture terminates the stream of stopping in first 1 day, culture 220~264h of period obtains the fermentation liquid containing K252A.
8. the method that fermentation medium according to claim 7 prepares K252A, it is characterised in that: the tank fermentation process It is middle stream plus glucose solution control strategy be maintain fermentation liquid in concentration of glucose within the scope of 1~5g/L, it is described stream plus The concentration of glucose solution is 500g/L.
9. the method according to claim 7 for preparing K252A, it is characterised in that: flow the function added in the tank fermentation process Energy factor mixed liquor ingredient are as follows: choline chloride 5g/L, disodium succinate 40g/L, methionine 130g/L, surplus are water；The stream The speed control of function factor mixed liquor is added to add volume to be the 0.05~0.1% of preliminary fermentation culture medium volume in stream per hour.
10. the method that fermentation medium according to claim 7 prepares K252A, it is characterised in that the shake-flask seed training Nutrient solution is obtained by following step:
1) it screens Norcardiopsis: using solid plate culture medium, select the big bacterium colony of Starch Hydrolysis circle；
2) prepare effective inoculation source: the excellent bacterium colony of picking is inoculated into solid slope culture medium, at 26~32 DEG C, 55~ Stationary culture 5 days under 65% relative humidity collect fresh, mature lawn and are used as effective inoculation source；
3) shake-flask seed culture: the provenance of step 2) is seeded in seed culture medium, at 26~32 DEG C, 150~330rpm item 24~72h is cultivated under part, obtains shake-flask seed culture solution；
The tank seed culture fluid passes through following step and obtains: by shake-flask seed culture solution, the seed that is first inoculated into seeding tank In culture medium, expands 18~96h of culture under the conditions of 26~32 DEG C, 50~300rpm, 0.35vvm, obtain tank seed culture fluid.
11. the method that fermentation medium according to claim 10 prepares K252A, it is characterised in that: described in step 1) Solid slope culture medium described in solid plate culture medium and step 2) includes the component of following weight percentage:
PH value is 6.5~7.5 before the solid medium sterilizes.
12. the method that fermentation medium according to claim 10 prepares K252A, it is characterised in that: described in step 3) Seed culture medium include following weight percentage component:
PH value is 6.5~7.5 before the seed culture medium sterilizes.
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