CN110468035B

CN110468035B - Sorting and filtering device for tumor cell clusters for oncology department

Info

Publication number: CN110468035B
Application number: CN201910709432.2A
Authority: CN
Inventors: 杨娅
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-08-02
Filing date: 2019-08-02
Publication date: 2022-07-08
Anticipated expiration: 2039-08-02
Also published as: CN110468035A

Abstract

The invention discloses a sorting and filtering device for tumor cell clusters for oncology, which comprises a bottom plate, wherein a lifting mechanism is arranged at the top of the bottom plate, a swinging mechanism is arranged at the top of the lifting mechanism, the lifting mechanism is in transmission connection with the swinging mechanism, and a filtering mechanism is arranged at the top of the swinging mechanism. According to the invention, through the matching of the lifting mechanism, the swinging mechanism and the filtering mechanism, the filtering mechanism can obtain a certain vibration quantity in a certain size and form after the driving mechanism is started, so that the sorting and filtering speed of the tumor cell mass is increased, and the sorting and filtering of the tumor cell mass is more thorough.

Description

Sorting and filtering device for tumor cell clusters for oncology department

Technical Field

The invention relates to the technical field of medical equipment, in particular to a sorting and filtering device for tumor cell clusters for oncology.

Background

The doctor of oncology often needs to sort tumour cell group in the course of working, has appeared on the market at present a device for sorting cell group, is used for specially sorting tumour cell.

For example, the chinese patent application No. CN201721033350.3 discloses a simple sorting and filtering device for tumor cell mass, which comprises a first filtering member, a second filtering member and a connecting pipe. The bottoms of the two filtering pieces are both filter screens; the top of the connecting pipe is provided with a funnel, the outer wall of the connecting pipe is provided with a pipe nozzle, and the position of the pipe nozzle is not lower than the height 1/3 above the bottom of the funnel. When the device is used, sample liquid sequentially passes through the first filtering piece and the second filtering piece, the first filtering piece filters large impurities such as tissue blocks, tumor cell masses are intercepted by the second filtering piece, and single cells and fine impurity particles pass through the second filtering piece and flow into the connecting pipe to be removed. The device can filter once to obtain cell clusters with uniform size, and can remarkably reduce the cell cluster quantity loss and the cell cluster activity damage caused by repeated operation; and the single cell and the fine impurities can be effectively removed, the interference of background signals generated in the subsequent test due to excessive fine impurity particles is avoided, the cell cluster sorting process is standardized on the whole, and the quality controllability of the test process is improved.

However, the simple sorting and filtering device for tumor cell mass has the following defects: this device can not give the vibration volume of certain size of tumour cell group and form when selecting separately the filtration for tumour cell group piles up easily and is difficult to filter out fast on the filter screen, also can shake the connecting pipe manually certainly, but wastes time and energy like this, and efficiency is very low.

Disclosure of Invention

The invention aims to provide a sorting and filtering device for tumor cell clusters for oncology, which has the advantages of rapidness and thoroughness in sorting and filtering, and solves the problems that the tumor cell clusters are easy to accumulate on a filter screen and difficult to rapidly filter out, and a connecting pipe can be manually shaken, but the problems of time and labor waste and low efficiency in the prior art are solved, and a certain size and form of vibration quantity cannot be given to the tumor cell clusters during sorting and filtering.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a tumour cell group's sorting filter equipment for oncology, includes the bottom plate, elevating system is installed at the top of bottom plate, the wabbler mechanism is installed at elevating system's top to the transmission is connected between elevating system and the wabbler mechanism, filter equipment is installed at the top of wabbler mechanism.

Preferably, the lifting mechanism comprises a lifting cylinder, the lifting cylinder is fixedly connected to the top of the bottom plate, the inner wall of the lifting cylinder is connected with a lifting column in a sliding mode, and a top plate is fixedly connected to the top end, far away from the lifting cylinder, of the lifting column.

Preferably, the swing mechanism comprises a support, the support is fixedly connected to the top of the top plate, a swing rod is hinged to the middle of the top of the support, a guide groove is formed in the surface, close to the bottom end, of the swing rod, a fixing plate is fixedly connected to the top, close to the end portion, of the top plate, a guide hole is formed in the side face, close to the top end, of the fixing plate, a guide rod is connected to the inner wall of the guide hole in a sliding mode, a guide block is fixedly connected to the front end of one end of the guide rod, the outer wall of the guide block is connected to the inner wall of the guide groove in a sliding mode, a fixing groove is formed in the bottom of the other end, far away from the guide block, of the guide rod, a rack is fixedly connected to the top of the end portion of the top plate, a first mounting seat is fixedly connected to the top of the end portion of the top plate, a driving mechanism is mounted at the front end of the top of the first mounting seat, and an incomplete gear is mounted to an output shaft of the driving mechanism, the surface of the incomplete gear is meshed with the surface of the rack.

Preferably, the front end of the bottom of the swing rod is hinged with a pull rod, the bottom end, far away from the swing rod, of the pull rod is hinged with a second mounting seat, and the second mounting seat is fixedly connected to the middle of the top of the bottom plate.

Preferably, the filtering mechanism includes a fixed cylinder, the top of guide way is kept away from to fixed cylinder fixed connection on the rocking bar, the top of fixed cylinder articulates there is fixed lid, the inner wall from the top down of fixed cylinder first filter screen of fixedly connected with, second filter screen and third filter screen in proper order, the outer wall installation and the intercommunication that is close to first filter screen, second filter screen and third filter screen respectively on the fixed cylinder has first bleeder valve, second bleeder valve and third bleeder valve, the outer wall that is close to its bottom on the fixed cylinder still installs and the intercommunication has the fourth bleeder valve.

Preferably, the driving mechanism is a speed reducer.

Preferably, the aperture of the first filter screen is 300-500 μm, the aperture of the second filter screen is 100-300 μm, and the aperture of the third filter screen is 10-100 μm.

Compared with the prior art, the invention has the following beneficial effects:

1. according to the tumor cell mass sorting device, the tumor cell mass is subjected to three-layer filtering through the first filter screen, the second filter screen and the third filter screen which are arranged in the filtering mechanism, and finally the sorted tumor cell mass is discharged through the first discharge valve, the second discharge valve, the third discharge valve and the fourth discharge valve.

2. According to the invention, the driving mechanism is started to drive the filtering mechanism to do swinging motion through the swinging mechanism, and the pull rod is used for driving the filtering mechanism to do lifting motion, so that a certain vibration quantity in a certain size and form is provided for the filtering mechanism, the filtering rate of the tumor cell clusters is further accelerated, the tumor cell clusters are sorted and filtered more thoroughly, and the sorting efficiency is improved.

Drawings

FIG. 1 is a schematic view of the filter mechanism of the present invention in a configuration swinging to the left;

FIG. 2 is a schematic structural view taken along the line A-A in FIG. 1;

FIG. 3 is a schematic structural view of a cross-sectional view of the filter mechanism of the present invention;

fig. 4 is a schematic view of the filter mechanism of the present invention when it is swung to the right.

In the figure: 1-bottom plate, 2-lifting mechanism, 21-lifting cylinder, 22-lifting column, 23-top plate, 3-rocking mechanism, 301-support, 302-rocking rod, 303-guide groove, 304-fixing plate, 305-guide hole, 306-guide rod, 310-guide block, 311-fixing groove, 312-rack, 313-a first mounting seat, 314-a driving mechanism, 315-an incomplete gear, 4-a filtering mechanism, 41-a fixed cylinder, 42-a fixed cover, 43-a first filter screen, 44-a second filter screen, 45-a third filter screen, 46-a first discharge valve, 47-a second discharge valve, 48-a third discharge valve, 49-a fourth discharge valve, 5-a pull rod and 6-a second mounting seat.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 4, the present invention provides a technical solution: the utility model provides a sorting filter equipment of tumour cell group for oncology, includes bottom plate 1, and elevating system 2 is installed at the top of bottom plate 1, and wabbler mechanism 3 is installed at the top of elevating system 2 to the transmission is connected between elevating system 2 and the wabbler mechanism 3, and filter mechanism 4 is installed at the top of wabbler mechanism 3.

Specifically, the filtering mechanism 4 includes a fixed cylinder 41, the fixed cylinder 41 is fixedly connected to the swing rod 302 and far away from the top end of the guide groove 303, the top of the fixed cylinder 41 is hinged with a fixed cover 42, and the fixed cover 42 and the fixed cylinder 41 can be locked, the inner wall of the fixed cylinder 41 is fixedly connected with a first filter 43, a second filter 44 and a third filter 45 from top to bottom in sequence, the aperture of the first filter 43 is preferably 300-500 μm, the aperture of the second filter 44 is preferably 100-300 μm, the aperture of the third filter 45 is preferably 10-100 μm, the fixed cylinder 41 is respectively provided with a first discharge valve 46, a second discharge valve 47 and a third discharge valve 48 which are communicated with the outer walls of the first filter 43, the second filter 44 and the third filter 45, the outer wall of the fixed cylinder 41 near the bottom thereof is further provided with a fourth discharge valve 49 which is communicated with the outer wall of the fixed cylinder 41, and the first filter 43, the second discharge valve 47 and the third discharge valve 48 are arranged in the filtering mechanism 4, Second filter screen 44 and third filter screen 45 carry out the three-layer filtration to tumor cell group, discharge the tumor cell group who selects separately through first bleeder valve 46, second bleeder valve 47, third bleeder valve 48 and fourth bleeder valve 49 at last to the quantity of filter screen is not limited to three in the present case, can also be subdivided into a plurality ofly for satisfying the sorting of not equidimension tumor cell group.

Specifically, the swing mechanism 3 includes a bracket 301, the bracket 301 is fixedly connected to the top of the top plate 23, a swing rod 302 is hinged to the middle of the top of the bracket 301, a guide groove 303 is formed on the surface of the swing rod 302 near the bottom end thereof, a fixing plate 304 is fixedly connected to the top of the top plate 23 near the end thereof, a guide hole 305 is formed on the side surface of the fixing plate 304 near the top end thereof, a guide rod 306 is slidably connected to the inner wall of the guide hole 305, a guide block 310 is fixedly connected to the front end of one end of the guide rod 306, the outer wall of the guide block 310 is slidably connected to the inner wall of the guide groove 303, a fixing groove 311 is formed on the bottom of the other end of the guide rod 306 far from the guide block 310, a rack 312 is fixedly connected to the top wall of the fixing groove 311, a first mounting seat 313 is fixedly connected to the top of the end of the top plate 23, a driving mechanism 314 is installed at the front end of the top of the first mounting seat 313, and an incomplete gear 315 is installed on an output shaft of the driving mechanism 314, the surface of the incomplete gear 315 is engaged and connected with the surface of the rack 312, when the driving mechanism 314 is started to drive the incomplete gear 315 to rotate reversely, the incomplete gear 315 is engaged with the rack 315 to drive the guide rod 306 to move left, the guide block 310 is abutted against the guide groove 303 to drive the swing rod 302 to rotate forward and backward to the state shown in fig. 4, otherwise when the driving mechanism 314 is started to drive the incomplete gear 315 to rotate forward and backward, the incomplete gear 315 is engaged with the rack 312 to drive the guide rod 306 to move right, the guide block 310 is abutted against the guide groove 303 to drive the swing rod 302 to rotate backward to the state shown in fig. 1, the incomplete gear 315 is continuously driven to rotate forward and backward to complete the swing of the filtering mechanism 4, so as to provide a certain vibration quantity in a certain size and form for the filtering mechanism 4, further accelerate the filtering rate of tumor cell clusters, and further enable the tumor cell clusters to be sorted and filtered more thoroughly, thereby improving the sorting efficiency, and the driving mechanism 314 is a speed reducer, which drives the filtering mechanism 4 to slowly operate at a low speed, so that the tumor cell mass is not severely impacted and damaged due to a high speed.

Specifically, elevating system 2 includes a lift section of thick bamboo 21, and a lift section of thick bamboo 21 fixed connection is at the top of bottom plate 1, and the inner wall sliding connection of a lift section of thick bamboo 21 has a lift post 22, and the top fixedly connected with roof 23 of keeping away from a lift section of thick bamboo 21 on the lift post 22. The front end of the bottom of the swing rod 302 is hinged with a pull rod 5, the bottom end, far away from the swing rod 302, of the pull rod 5 is hinged with a second mounting seat 6, the second mounting seat 6 is fixedly connected to the middle of the top of the bottom plate 1, during the swing of the filtering mechanism 4, due to the height change of the bottom end of the swing rod 302 in the vertical direction, the filtering mechanism 4 can be driven to swing and do lifting movement at the same time through the pull rod 5, and therefore a certain size and form of vibration quantity is provided for the filtering mechanism 4, and the filtering rate of tumor cell clusters is further increased; after the sorting and filtering, the state is adjusted to the state in fig. 4, at this time, the sorted and filtered tumor cell mass is easier to discharge because the first discharge valve 46, the second discharge valve 47, the third discharge valve 48 and the fourth discharge valve 49 are inclined downwards.

The working principle is as follows: when the sorting and filtering device for the tumor cell mass for the oncology department is used, firstly, the fixing cover 42 is opened to pour the tumor cell mass into the fixing cylinder 41, the fixing cover 42 is closed, then the driving mechanism 314 is started to drive the incomplete gear 315 to rotate reversely, the incomplete gear 315 is meshed with the rack 315 to drive the guide rod 306 to move left, the guide block 310 is propped against the guide groove 303 to drive the swing rod 302 to rotate forward and forward to the state shown in figure 4, on the contrary, when the driving mechanism 314 is started to drive the incomplete gear 315 to rotate forward and forward, the incomplete gear 315 is meshed with the rack 312 to drive the guide rod 306 to move right, the guide block 310 is propped against the guide groove 303 to drive the swing rod 302 to rotate backward to the state shown in figure 1, the incomplete gear 315 is continuously driven to rotate forward and backward to complete the swing of the filtering mechanism 4, so that a certain vibration quantity with a certain size and form is provided for the filtering mechanism 4, and the filtering rate of the tumor cell mass is further accelerated, the tumor cell mass sorting and filtering are more thorough, the sorting efficiency is improved, the driving mechanism 314 is a speed reducer, the driving mechanism 4 is driven to slowly carry out at a low speed, the tumor cell mass is not seriously impacted and damaged because of the high speed, and during the swinging of the filtering mechanism 4, the height change of the bottom end of the swinging rod 302 in the vertical direction can drive the filtering mechanism 4 to swing and simultaneously carry out lifting motion through the pull rod 5, so that a certain vibration quantity with a certain size and form is provided for the filtering mechanism 4, and the filtering speed of the tumor cell mass is further increased; after the sorting and filtering, the state is adjusted to the state in fig. 4, at this time, the sorted and filtered tumor cell mass is more easily discharged because the first discharge valve 46, the second discharge valve 47, the third discharge valve 48 and the fourth discharge valve 49 are inclined downwards.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a sorting filter equipment of tumour cell group for oncology, includes bottom plate (1), its characterized in that: the top of the bottom plate (1) is provided with a lifting mechanism (2), the top of the lifting mechanism (2) is provided with a swing mechanism (3), the lifting mechanism (2) is in transmission connection with the swing mechanism (3), and the top of the swing mechanism (3) is provided with a filtering mechanism (4);

the lifting mechanism (2) comprises a lifting cylinder (21), the lifting cylinder (21) is fixedly connected to the top of the bottom plate (1), the inner wall of the lifting cylinder (21) is connected with a lifting column (22) in a sliding mode, and a top plate (23) is fixedly connected to the top end, far away from the lifting cylinder (21), of the lifting column (22);

the swing mechanism (3) comprises a support (301), the support (301) is fixedly connected to the top of the top plate (23), a swing rod (302) is hinged to the middle of the top of the support (301), a guide groove (303) is formed in the surface, close to the bottom end, of the swing rod (302), a fixing plate (304) is fixedly connected to the top, close to the end portion, of the top plate (23), a guide hole (305) is formed in the side face, close to the top end, of the fixing plate (304), the inner wall of the guide hole (305) is slidably connected with a guide rod (306), a guide block (310) is fixedly connected to the front end of one end of the guide rod (306), the outer wall of the guide block (310) is slidably connected with the inner wall of the guide groove (303), a fixing groove (311) is formed in the bottom of the other end, far away from the guide block (310), of the guide rod (306), and a rack (312) is fixedly connected to the top wall of the fixing groove (311), the top of the end part of the top plate (23) is fixedly connected with a first mounting seat (313), the front end of the top of the first mounting seat (313) is provided with a driving mechanism (314), an output shaft of the driving mechanism (314) is provided with an incomplete gear (315), and the surface of the incomplete gear (315) is meshed with the surface of the rack (312);

the front end of the bottom of the swing rod (302) is hinged with a pull rod (5), the bottom end, far away from the swing rod (302), of the pull rod (5) is hinged with a second mounting seat (6), and the second mounting seat (6) is fixedly connected to the middle of the top of the bottom plate (1);

filtering mechanism (4) are including solid fixed cylinder (41), gu fixed cylinder (41) fixed connection keeps away from the top of guide way (303) on rocking bar (302), gu the top of fixed cylinder (41) articulates there is fixed lid (42), the inner wall from the top down of fixed cylinder (41) is first filter screen (43), second filter screen (44) and third filter screen (45) of fixedly connected with in proper order, gu be close to the outer wall installation and the intercommunication of first filter screen (43), second filter screen (44) and third filter screen (45) on fixed cylinder (41) respectively and have first bleeder valve (46), second bleeder valve (47) and third bleeder valve (48), gu the outer wall that is close to its bottom on fixed cylinder (41) still installs and the intercommunication has fourth bleeder valve (49).

2. The apparatus for sorting and filtering tumor cell mass for oncology according to claim 1, wherein: the driving mechanism (314) is a speed reducer.

3. The apparatus for sorting and filtering tumor cell mass for oncology department according to claim 1, wherein: the aperture of the first filter screen (43) is 300-500 mu m, the aperture of the second filter screen (44) is 100-300 mu m, and the aperture of the third filter screen (45) is 10-100 mu m.