CN110467274A - The new opplication of acidovorax facilis LS-1 and kit and its application for repairing arsenic pollution - Google Patents
The new opplication of acidovorax facilis LS-1 and kit and its application for repairing arsenic pollution Download PDFInfo
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- CN110467274A CN110467274A CN201910796471.0A CN201910796471A CN110467274A CN 110467274 A CN110467274 A CN 110467274A CN 201910796471 A CN201910796471 A CN 201910796471A CN 110467274 A CN110467274 A CN 110467274A
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- acidovorax facilis
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/103—Arsenic compounds
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
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Abstract
Kit and its application the present invention relates to the new opplication of acidovorax facilis LS-1 a kind of and for repairing arsenic pollution.The new opplication is that acidovorax facilis LS-1 is repairing the application in arsenic pollution, under anaerobic, acidovorax facilis LS-1 is added in arsenic pollution object, pentavalent arsenic is reduced to trivalent arsenic, the deposit number of acidovorax facilis LS-1 is CGMCC NO:11555.The research of the invention finds that acidovorax facilis LS-1 has the ability that pentavalent arsenic is reduced to trivalent arsenic under anaerobic, the bioavailability of trivalent arsenic, mobility are significantly larger than pentavalent arsenic, thus the activity of arsenic in the environment can be improved in processing in this way, is conducive to subsequent by trivalent arsenic removing or recycling.It is proposed by the present invention to apply acidovorax facilis LS-1 in arsenic pollution improvement, pentavalent arsenic can be made to be reduced to trivalent arsenic to be easier to have a good application prospect by rich absorbent, thus in terms of repairing arsenic pollution.
Description
Technical field
The present invention relates to technical field of environmental microorganism, a kind of new opplication more particularly to acidovorax facilis LS-1 and are used for
Repair kit and its application of arsenic pollution.
Background technique
Arsenic is a kind of noxious material being widely present in the natural environments such as soil, water body, not only decline soil fertility,
Crop yield, quality decline, also result in water pollution.The approach that people absorb arsenic is mainly the pollution of long-term drinking High Concentration of Arsenic
Drinking water and the edible food containing arsenic, cause arsenic finally to accumulate in human body by the effect of food chain, seriously affect people
The health of class.
Mainly there are engineering reparation, physical chemistry reparation and microorganism remediation for the recovery technique that arsenic pollution status is taken.
Since microculture is simple, corresponding preparation is at low cost, it is good to repair arsenic pollution effect, rapid-action, timeliness is long, not will cause two
Secondary pollution, therefore, microorganism remediation are the ideal methods for repairing arsenic pollution.But also need further to screen at present it is more,
Functionally more powerful microbe species, to play effect more outstanding in repairing arsenic pollution.
Summary of the invention
Based on this, it is necessary to aiming at the problem that arsenic pollution, provide the new opplication of acidovorax facilis LS-1 a kind of and for repairing
The kit of arsenic pollution and its application.
A kind of new opplication of acidovorax facilis LS-1, the new opplication are that the acidovorax facilis LS-1 is repairing answering in arsenic pollution
With under anaerobic, the acidovorax facilis LS-1 being added in arsenic pollution object, pentavalent arsenic is reduced to trivalent arsenic, the acidovorax facilis
The deposit number of LS-1 is CGMCC NO:11555.
It is that the bacterium solution containing the acidovorax facilis LS-1 is added to the arsenic in one of the embodiments, when repairing
In pollutant, the use concentration of the acidovorax facilis LS-1 in the bacterium solution is 104A/mL~106A/mL.
The amount ratio of the bacterium solution and the arsenic pollution object is 1mL:(10mL~20mL in one of the embodiments)；
The processing time for repairing arsenic pollution is greater than 0 day and is less than or equal to 6 days.
In one of the embodiments, when repairing, further include control nitrate ion in the arsenic pollution object and/or
Step of the ferrous ion within the scope of preset concentration.
The content of pentavalent arsenic is not more than 1mmol/L in the arsenic pollution object in one of the embodiments,.
In one of the embodiments, when repairing, the pH value of control reparation system is 6~8.
The arsenic pollution object is water body arsenic pollution object and/or arsenic in soil pollutant in one of the embodiments,.
The present invention also provides a kind of for repairing the kit of arsenic pollution, including arsenic pollution preparation for repairing, the arsenic pollution
Preparation for repairing is the fungi medicament containing acidovorax facilis LS-1, and the deposit number of the acidovorax facilis LS-1 is CGMCC NO:11555.
It further include in one of the embodiments, pH adjusting agent, nitrate anion remover for repairing the kit of arsenic pollution
With at least one of ferrous ion remover reagent.
The present invention also provides the kits for repairing arsenic pollution to repair the application in arsenic pollution.
The research of the invention finds that acidovorax facilis LS-1 has the ability of trivalent arsenic of being under anaerobic reduced to pentavalent arsenic, three
The bioavailability of valence arsenic, mobility be significantly larger than pentavalent arsenic, thus arsenic can be improved in the environment in processing in this way
Activity is conducive to subsequent by trivalent arsenic removing or recycling.
It is proposed by the present invention to apply acidovorax facilis LS-1 in arsenic pollution improvement, pentavalent arsenic can be made to be reduced to trivalent
Arsenic is to be easier to have a good application prospect by rich absorbent, thus in terms of repairing arsenic pollution.
Detailed description of the invention
Fig. 1 is the transmission electron microscope picture of bacterial strain of the present invention；
Fig. 2 is the systematic growth tree graph of the 16S rRNA sequence construct of bacterial strain of the present invention；
Fig. 3 is growth curve chart of the bacterial strain of the present invention in various concentration AA (V)；
Fig. 4 is that bacterial strain of the present invention restores conversion figure to AA (V)；
Fig. 5 is that the arsenic of bacterial strain of the present invention restores gene electrophoretogram；
Fig. 6 is for bacterial strain of the present invention to the curve graph of arsenic reduction under the conditions of nitrate ion, ferrous ion.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, to specific embodiment party of the invention
Formula is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But this hair
Bright to be implemented with being much different from other way described herein, those skilled in the art can be without prejudice in the present invention
Similar improvement is done in the case where culvert, therefore the present invention is not limited by the specific embodiments disclosed below.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
The present invention provides the new opplication of acidovorax facilis LS-1 a kind of, and new opplication is that acidovorax facilis LS-1 is repairing answering in arsenic pollution
With under anaerobic, acidovorax facilis LS-1 being added in arsenic pollution object, pentavalent arsenic is reduced to trivalent arsenic, the guarantor of acidovorax facilis LS-1
Hiding number is CGMCC NO:11555.
The principle that acidovorax facilis LS-1 repairs arsenic pollution is as follows: under anaerobic, acidovorax facilis LS-1 has stronger arsenic resistance to
By characteristic, it can survive and grow in the arsenic environment of high concentration, and the bacterium has arsenic also protogene, which is added arsenic pollution
In object, pentavalent arsenic can not only be reduced to trivalent arsenic, and thallus itself can survive in arsenic-containing atmosphere well and grow, with
Reach effective and repairs High Concentration of Arsenic pollution.
It is that the bacterium solution containing acidovorax facilis LS-1 is added into arsenic pollution object when repairing in a wherein embodiment,
The use concentration of acidovorax facilis LS-1 in bacterium solution is 104A/mL~106A/mL.Bacterium solution and the amount ratio of the arsenic pollution object are
1mL:(10mL~20mL).The processing time for repairing arsenic pollution, which is greater than 0 day, to be less than or equal to 6 days.Under anaerobic, acidovorax facilis
LS-1 has stronger resistance characteristics to arsenic, can grow in the environment containing arsenic, using the acidovorax facilis LS-1 of above-mentioned concentration, and
Setting and arsenic pollution object reasonable volume ratio, just can be reduced to trivalent arsenic for the pentavalent arsenic in arsenic pollution object in 6 days.Trivalent arsenic
It is stronger relative to pentavalent arsenic bioavailability, mobility, it is easier to be absorbed and used by plants, this is in phytoremediation heavy metal arsenic
It is of great significance in pollution, achievees the purpose that repair arsenic pollution.
It further include the nitrate ion and/or ferrous iron controlled in arsenic pollution object when repairing in a wherein embodiment
Step of the ion within the scope of preset concentration.The research of the invention finds that in nitrate ion and/or without item existing for ferrous ion
Under part, pentavalent arsenic is reduced to trivalent arsenic by inhibition acidovorax facilis LS-1 that can be different degrees of.
Preferably, when repairing, the nitrate anion and ferrous ion in arsenic pollution object are removed.
In a wherein embodiment, the content of pentavalent arsenic is not more than 1mmol/L in arsenic pollution object.
In a wherein embodiment, when repairing, the pH value of control reparation system is 6~8.The bacterium is bismuthite
Ferrous oxidation bacterium is restored, the most suitable growth pH is neutrality.
In a wherein embodiment, arsenic pollution object is water body arsenic pollution object and/or arsenic in soil pollutant.
The present invention also provides an embodiments for repairing the kit of arsenic pollution, including arsenic pollution preparation for repairing.Its
In, arsenic pollution preparation for repairing is the fungi medicament containing acidovorax facilis LS-1, and the deposit number of acidovorax facilis LS-1 is CGMCC NO:
11555。
In a wherein embodiment, the kit for repairing arsenic pollution further includes pH adjusting agent, nitrate anion remover
With at least one of ferrous ion remover reagent.Wherein, pH adjusting agent carries out adaptability tune according to different acid or alkali environments
Whole, when arsenic pollution object is in strong acidity, i.e. pH < 6, the pH adjusting agent is alkaline matter, when arsenic pollution object is in compared with strong basicity
When, i.e. pH > 8, the pH adjusting agent is acidic materials.
The present invention also provides the above-mentioned kits for repairing arsenic pollution of an embodiment in repairing arsenic pollution
Using.
Below with reference to embodiment, the present invention is further explained:
The separation screening of embodiment 1, acidovorax facilis LS-1
Place acquisition soil sample from the Chinese Academy of Sciences, Guangdong Province south China plant institute's water paddy soil surface layer depths 10-20cm,
Then the soil sample of acquisition and liquid selective medium are subjected to anaerobism with the cillin bottle that the Tu Shui ratio of 1:100 (g/L) is placed in 100mL
Culture.Being passed through high pure nitrogen 30min makes to keep abundant anaerobic state in system, then rubber stopper and aluminium lid on fast ram compression again, sets
Culture is protected from light in 30 DEG C of constant-temperatureanaerobic anaerobic casees.Culture is transferred to after a week, by the liquid in cillin bottle with the ratio of 1:10 new
It in liquid selective medium, is cultivated under the above conditions, such repeated inoculation enrichment culture 3-5 times.
It is 10 by concentration by enrichment culture liquid by ten times of gradient dilution method dilutions in anaerobism work station-1、10-2、10-3、
10-4、10-5Dilution respectively draw 0.1mL, be uniformly coated on solid plate culture medium respectively (in liquid selective medium
The agar of 15g/L is added), the plate after inoculation is inverted in 30 DEG C of constant-temperatureanaerobic anaerobic casees and is cultivated to growing new bacterium colony, selection is in
Colony lift russet is isolated and purified to obtain pure bacterial strain into fresh liquid selective medium, and bacterium solution is taken to go out in 30%
Bacterium glycerol is stored in -80 DEG C.Wherein, used liquid selective medium includes: 10mM NaNO3, 5mM FeCl2.4H2O,
30mM Piperazine-N, N '-biA (2-ethaneAulfonic acid) (PIPES pH of buffer 7.0 ± 0.2), 5mM second
Sour sodium and 1mL/L microelement and 1mL/L vitamin.
The identification of 2 bacterial strain of embodiment
(1) Morphological Identification
Judged by observing colonial morphology of the bacterial strain for screening and obtaining on solid plate culture medium, it is aobvious using optics
Micro mirror carries out microscopic morphology observation to bacterium colony.As shown in Figure 1, the bacterial strain is in corynebacterium, it is about 1-2 μm, it is about 0.3 μm wide, and
With flagellum.
(2) molecular biology identification
The extraction of bacterial strain DNA: the strain of preservation is inoculated into liquid selective medium, is placed in 30 DEG C of constant-temperatureanaerobic anaerobic casees
It is protected from light culture, culture solution is taken out in culture after 6 days, 8000r/min is centrifuged 5min, abandons supernatant collection and precipitates thallus.Use DNA of bacteria
Extracts kit EZNATM Bacterial DNA Kit (Omega, USA) extracts the total DNA of separated bacterial strain, the DNA of extraction
It is saved in -20 DEG C.
PCR amplification: using universal primer 27F (5 '-AGAGTTTGATCMTGGCTCAG-3 ', SEQ ID No.1) and
The 16S rRNA gene of 1492R (5 '-GGTTACCTTGTTACGACTT-3 ', SEQ ID No.2) amplification bacterium.
Amplification condition: 94 DEG C of initial denaturation 4min；94 DEG C of denaturation 30A, 55 DEG C of annealing 45A, 72 DEG C of extension 60A, totally 30 are followed
Ring.
Gel electrophoresis: 1.5% agarose gel electrophoresis of pcr amplification product is detected into (1 × TAE of electrophoretic buffer, voltage
150V/cm, time about 30min) and give to company and be sequenced.Measure the sequence (SEQ ID No.3) of its 16S rRNA gene
It is as follows:
CATGCAGTCGACGGTAACAGGTCTTCGGATGCTGACGAGTGGCGAACGGGTGAGTAATACATCGGAACG
TGCCCGATCGTGGGGGATAACGAGGCGAAAGCTTTGCTAATACCGCATACGATCTACGGATGAAAGCGGGGGATCTT
CGGACCTCGCGCGGACGGAGCGGCCGATGGCAGATTAGGTAGTTGGTGGGATAAAAGCTTACCAAGCCGACGATCTG
TAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGA
ATTTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCTTTT
GTACGGAACGAAAAGCCTCTTTCTAATAAAGAGGGGTCATGACGGTACCGTAAGAATAAGCACCGGCTAACTACGTG
CCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTTGT
AAGACAGAGGTGAAATCCCCGGGCTCAACCTGGGAACTGCCTTTGTGACTGCAAGGCTGGAGTGCGGCAGAGGGGGA
TGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATGCGGAGGAACACCGATGGCGAAAGCAATCCCCTGGGCCTGC
ACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTGCACGCCCTAAACGATGTCAA
CTGGTTGTTGGGTCTTCACTGACTCAGTAACGAAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGCCGCAAGG
TTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGTTTAATTCGATGCAACGCGAAAAACC
TTACCCACCTTTGACATGGCAGGAAGTTTCCAGAGATGGATTCGTGCCCGAAAGGGAACCTGCACACAGGTGCTGCA
TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGCCATTAGTTGCTACG
AAAGGGCACTCTAATGGGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATA
GGTGGGGCTACACACGTCATACAATGGCTGGTACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCATAAAGCC
AGTCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGGATCAGAATGTCGC
GGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCGGGTTCTGCCAGAAGTAGGTAGCCTA
ACCGTAAGGAGGCGCTA
Sequence is submitted in EzBioCloud database and carries out tetraploid rice, the similarity letter of analysis purpose bacterial strain
Breath, is compared the classification position of determining bacterial strain.Correlated series are chosen according to comparison result, calculate structure using Mega6.0N-J method
Build phylogenetic tree.It is as shown in Figure 2 to construct obtained 16S rRNA phylogenetic tree, the results showed that the sequence and acidovorax facilis TPSY
The 16S rDNA gene order similarity of (Acidovorax ebreus) is up to 99% or more, and classification naming is acidovorax facilis
(Acidovorax ebreus) LS-1 is preserved in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 on October 29th, 2015
The China Committee for Culture Collection of Microorganisms's common micro-organisms center of Institute of Microorganism, Academia Sinica, deposit number are
CGMCC NO:11555。
Embodiment 3, bacterial strain are to the resistance characteristics of AA (V)
Acidovorax facilis (Acidovorax ebreus) LS-1 is cultivated in anaerobism LB culture medium to logarithmic phase, then with going out
The fluid nutrient medium of bacterium washes bacterium 2-3 times, and mother bacterial liquid is made.The mother bacterial liquid of equivalent is taken to be inoculated into respectively containing different AA (V) concentration
In the fluid nutrient medium of (0,200,500,1000 μM), its OD600 is once surveyed per sampling for 24 hours, records and draws various concentration AA
(V) the OD600 curve changed over time under the conditions of.Wherein, fluid nutrient medium includes: Piperazine-N, N '-biA (2-
EthaneAulfonic acid) (PIPES pH of buffer 7.0 ± 0.2), 5mM sodium acetate and 1mL/L microelement and 1mL/L
Vitamin.
Experimental result is as shown in Figure 3, the results showed that the bacterial strain has stronger resistance to arsenic ability, and tolerance arsenic concentration is up to 1mM.
Embodiment 4, bacterial strain are to the reduction characteristic of AA (V)
The preparation of fluid nutrient medium and the production of mother bacterial liquid are the same as embodiment 3.
The mother bacterial liquid of 2mL logarithmic phase is inoculated in the fluid nutrient medium of the sterilizing containing 1mM AA (V), wherein in mother bacterial liquid
The content of bacterium is about 106A/mL, drum fill high pure nitrogen 30min removal oxygen, and air-blowing finishes rear cover serum cap and aluminium lid is added to seal,
It is cultivated in 30 DEG C of standing anaerobic culture boxes.It was sampled respectively at 0,1,2,4,6,9 day, and with 0.22 μm of membrane filtration, uses atom
Fluorescence analyser (Ji Tian, China) surveys AA (V) and AA (III) content.
Experimental result is as shown in Figure 4, the results showed that arsenic AA (V) can be reduced to AA (III) by the bacterial strain, in culture the 4th
It when existing 80% or so AA (V) be reduced, AA (V) is almost completely reduced when by the 6th day.
The arsenic reduction gene magnification of embodiment 5, acidovorax facilis Acidovorax ebreus LS-1
Arsenic restores gene PCR amplification: using primer amlt-42F (TCGCGTAATACGCTGGAGAT, SEQ ID No.4)
With the arsenic also protogene of amlt-376R (ACTTTCTCGCCGTCTTCCTT, SEQ ID No.5) amplification bacterium.
Amplification condition: 94 DEG C of initial denaturation 4min, 94 DEG C of denaturation 30A, 60 DEG C of annealing 45A, 72 DEG C of extension 60A, totally 30 are followed
Ring.
Gel electrophoresis: 1.0% agarose gel electrophoresis of pcr amplification product is detected into (1 × TAE of electrophoretic buffer, voltage
150V/cm, time about 30min), PCR amplification obtains adhesive tape as shown in figure 5, swimming lane M is the marker of 2000bp；Arsenic aoxidizes base
Because the amplified fragments of arAC are about 330bp.
The influence factor of embodiment 6, acidovorax facilis Acidovorax ebreus LS-1 arsenic reduction
The preparation of fluid nutrient medium and the production of mother bacterial liquid are the same as embodiment 3.
Mother bacterial liquid is inoculated in fluid nutrient medium, three different systems are set, respectively 1) bacterial strain+arsenate+sodium nitrate；
2) bacterial strain+arsenate+FeCl2·4H2O；3) bacterial strain+arsenate+sodium nitrate+FeCl2·4H2O；Wherein NaNO3、FeCl2·
4H2The initial concentration of O and arsenate is respectively 10mM, 5mM, 1mM, and drum fills high pure nitrogen 30min discharge oxygen, after air-blowing
Lid serum cap adds aluminium lid to seal, in 30 DEG C of standing Anaerobic culturels.
It was sampled respectively at 0,1,2,4,6,9 day, and with 0.22 μm of membrane filtration atomic fluorescence analysis instrument (Ji Tian, Chinese)
Survey the content of AA (V), AA (III).
Experimental result is as shown in fig. 6, after culture 9 days, and in the system of addition nitrate, the reduction of arsenic is obviously inhibited,
Only a small amount of AA (V) is reduced, and the reduction rate of AA (V) is only 10% or so.In the system of addition ferrous ion, AA
(V) reduction is also suppressed, and the reduction rate of AA (V) is 70% or so.The system of nitrate and ferrous ion is added simultaneously
In, the generation of AA (III) is not detected in solution, the reduction rate of AA (V) is less than 10%.Nitrate indicated above and it is ferrous from
The addition of son has different degrees of inhibiting effect to bacterial strain reduction arsenic.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Sequence table
<110>Bioengineering Research Institute, Guangdong Province (Guangzhou Inst of Cane Sugar)
<120>new opplication of acidovorax facilis LS-1 and kit and its application for repairing arsenic pollution
<160> 5
<170> SIPOSequenceListing 1.0
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catgcagtcg acggtaacag gtcttcggat gctgacgagt ggcgaacggg tgagtaatac 60
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atctacggat gaaagcgggg gatcttcgga cctcgcgcgg acggagcggc cgatggcaga 180
ttaggtagtt ggtgggataa aagcttacca agccgacgat ctgtagctgg tctgagagga 240
tgatcagcca cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga 300
attttggaca atgggcgaaa gcctgatcca gccatgccgc gtgcaggatg aaggccttcg 360
ggttgtaaac tgcttttgta cggaacgaaa agcctctttc taataaagag gggtcatgac 420
ggtaccgtaa gaataagcac cggctaacta cgtgccagca gccgcggtaa tacgtagggt 480
gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg taagacagag 540
gtgaaatccc cgggctcaac ctgggaactg cctttgtgac tgcaaggctg gagtgcggca 600
gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag gaacaccgat 660
ggcgaaagca atcccctggg cctgcactga cgctcatgca cgaaagcgtg gggagcaaac 720
aggattagat accctggtag tgcacgccct aaacgatgtc aactggttgt tgggtcttca 780
ctgactcagt aacgaagcta acgcgtgaag ttgaccgcct ggggagtacg gccgcaaggt 840
tgaaactcaa aggaattgac ggggacccgc acaagcggtg gatgatgtgg tttaattcga 900
tgcaacgcga aaaaccttac ccacctttga catggcagga agtttccaga gatggattcg 960
tgcccgaaag ggaacctgca cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga 1020
tgttgggtta agtcccgcaa cgagcgcaac ccttgccatt agttgctacg aaagggcact 1080
ctaatgggac tgccggtgac aaaccggagg aaggtgggga tgacgtcaag tcctcatggc 1140
ccttataggt ggggctacac acgtcataca atggctggta cagagggttg ccaacccgcg 1200
agggggagct aatcccataa agccagtcgt agtccggatc gcagtctgca actcgactgc 1260
gtgaagtcgg aatcgctagt aatcgcggat cagaatgtcg cggtgaatac gttcccgggt 1320
cttgtacaca ccgcccgtca caccatggga gcgggttctg ccagaagtag gtagcctaac 1380
cgtaaggagg cgcta 1395
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Claims (10)
1. a kind of new opplication of acidovorax facilis LS-1, which is characterized in that the new opplication is that the acidovorax facilis LS-1 is repairing arsenic dirt
The acidovorax facilis LS-1 is added in arsenic pollution object pentavalent arsenic being reduced to trivalent arsenic by the application in dye under anaerobic, institute
The deposit number for stating acidovorax facilis LS-1 is CGMCC NO:11555.
2. the new opplication of acidovorax facilis LS-1 according to claim 1, which is characterized in that when repairing, being will be containing described
The bacterium solution of acidovorax facilis LS-1 is added into the arsenic pollution object, and the use concentration of the acidovorax facilis LS-1 in the bacterium solution is
104A/mL~106A/mL.
3. the new opplication of acidovorax facilis LS-1 according to claim 2, which is characterized in that the bacterium solution and the arsenic pollution object
Amount ratio be 1mL:(10mL~20mL)；The processing time for repairing arsenic pollution is greater than 0 day and is less than or equal to 6 days.
4. the new opplication of acidovorax facilis LS-1 according to claim 1, which is characterized in that further include control institute when repairing
State the step of nitrate ion and/or ferrous ion within the scope of preset concentration in arsenic pollution object.
5. the new opplication of acidovorax facilis LS-1 according to claim 1, which is characterized in that pentavalent arsenic in the arsenic pollution object
Content is not more than 1mmol/L.
6. the new opplication of acidovorax facilis LS-1 according to claim 1, which is characterized in that when repairing, control reparation system
PH value be 6~8.
7. the new opplication of acidovorax facilis LS-1 according to claim 1, which is characterized in that the arsenic pollution object is that water body arsenic is dirty
Contaminate object and/or arsenic in soil pollutant.
8. a kind of for repairing the kit of arsenic pollution, which is characterized in that including arsenic pollution preparation for repairing, the arsenic pollution reparation
Preparation is the fungi medicament containing acidovorax facilis LS-1, and the deposit number of the acidovorax facilis LS-1 is CGMCC NO:11555.
9. as claimed in claim 8 for repairing the kit of arsenic pollution, which is characterized in that further include pH adjusting agent, nitric acid
At least one of root remover and ferrous ion remover reagent.
10. repairing the application in arsenic pollution for repairing the kit of arsenic pollution as claimed in claim 8 or 9.
Priority Applications (1)
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