CN110448727A - A kind of cohesive hydrogel material exempts to suture artificial nerve catheter and preparation method thereof - Google Patents

A kind of cohesive hydrogel material exempts to suture artificial nerve catheter and preparation method thereof Download PDF

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CN110448727A
CN110448727A CN201910874185.1A CN201910874185A CN110448727A CN 110448727 A CN110448727 A CN 110448727A CN 201910874185 A CN201910874185 A CN 201910874185A CN 110448727 A CN110448727 A CN 110448727A
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hydrogel material
artificial nerve
preparation
mixed solution
cohesive hydrogel
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CN110448727B (en
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凌珏
杨宇民
汤晓璇
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Nantong University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction

Abstract

The invention belongs to the biomaterial for medical purpose field in human implantable, discloses a kind of cohesive hydrogel material, exempts to suture artificial nerve catheter and preparation method thereof.The present invention by with tissue adhension function cohesive hydrogel material and chitosan artificial nerve catheter combine, provide it is a kind of from artificial operation stitching, it is convenient to use to exempt to suture artificial nerve catheter.Being bonded in the cohesive hydrogel material for exempting to suture artificial nerve catheter end side has good histocompatbility and mechanical property, it being capable of cumbersome sewing process by physical action and chemical bonding and wet tissue surface strong binding, when can be to avoid operation implantation catheter.Exempt to suture artificial nerve catheter to prepare this, the invention also discloses a kind of preparation methods that is simple and easy, being suitble to production.

Description

A kind of cohesive hydrogel material exempts to suture artificial nerve catheter and preparation method thereof
Technical field
The invention belongs in human implantable biomaterial for medical purpose field more particularly to a kind of cohesive hydrogel material, Exempt to suture artificial nerve catheter and preparation method thereof.
Background technique
In China, peripheral nerve injury patient populations are in the trend increased year by year.Although peripheral nerve has oneself certain My repair function, but it is difficult to realize complete self-regeneration especially long range neurologic defect, it is therefore desirable to by means of various nerves Bridge material is repaired.Although Autologous bracket is the goldstandard for repairing peripheral nerve defection, but is faced with donor The problems such as source is limited, and size mismatches and can cause permanent injury to materials position.Currently, people mainly by by have compared with The various tissue engineering nerve bridge materials that the natural or synthetic biomaterial of good biocompatibility and biological degradability is prepared To realize neural restoration.But these artificial nerve grafts promote the speed of peripheral nerve regeneration to be still difficult to meet clinical need It wants, is especially difficult to repair the neurologic defect of relatively long distance.Therefore, exploitation can comparatively fast promote peripheral nerve regeneration and be able to achieve compared with The new type nerve graft of long range neurologic defect reparation has very important scientific meaning.
Hydrogel is highly cross-linked hydrophilic polymer, and structure is similar to natural extracellular matrix, has good biological Compatibility has been widely studied, and has been applied in the field of medicine.Naturally occurring polymer such as chitosan, alginates, Hyaluronic acid (HA), collagen and gelatin etc. have biodegradable, but there are bad mechanical property, immunogenicity and batches Between otherness the disadvantages of.And polyethylene glycol (PEG), polyacrylamide (PAM), poly- (vinyl alcohol) (PVA) and polymethyl The synthesis quasi polymer such as sour methyl esters (PMMA) generally has more good mechanical performance and low immunogenicity, but lacks biological function Energy.Therefore by natural and synthesize the hydrogel that quasi polymer is compounded to form and can satisfy vivo applications.
To be adhered to many fields all critically important to wet and dynamic surface (including biological tissue), can be arrived with strongly bonded The adhesive of biological tissue will have a wide range of applications, from tissue repair and drug delivery.However, existing tissue adhesive is remote It is non-ideal, or have cytotoxicity, histocompatbility poor, or in wet tissue surface poor adhesion.Therefore being badly in need of exploitation can be Wet tissue surface has the biomaterial of good adhesive property and histocompatbility.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of cohesive hydrogel material, exempt from suture artificial nerve catheter and Preparation method.Cohesive hydrogel material can realize artificial nerve catheter and wet and dynamic organization surface bonding, without seam It closes, it is easy to use.
In order to achieve the object of the present invention, the invention adopts the following technical scheme:
A kind of preparation method of cohesive hydrogel material, comprising:
1) chitosan, sodium alginate and acrylamide are dissolved in MES buffer, obtain mixed solution；
2) 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride and N- hydroxyl are added in Xiang Suoshu mixed solution Succinimide after being uniformly mixed, adds N, N'- methylene-bisacrylamide, tetramethylethylenediamine, ammonium persulfate and CaSO4, 0.5~1h is stirred at room temperature, obtains cohesive hydrogel material.
Preferably, the pH value of the MES buffer is 5.
Preferably, each component content in the mixed solution are as follows:
It is furthermore preferred that each component content in the mixed solution are as follows:
Preferably, the usage amount of 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride is every in step (2) 0.12g is added in the mixed solution of 1mL；The usage amount of n-hydroxysuccinimide is in the mixed solution of every 1mL 0.12g is added；3.6 μ L concentration are added in the mixed solution that the usage amount of N, N'- methylene-bisacrylamide is every 1mL is The N of 20g/L, N'- methylene-bisacrylamide；It is added in the mixed solution that the usage amount of tetramethylethylenediamine is every 1mL The tetramethylethylenediamine of 0.8 μ L；The concentration of 22.6 μ L is added in the mixed solution that the dosage of ammonium persulfate is every 1mL The ammonium persulfate of 0.27M；CaSO4Dosage be every 1mL the mixed solution in the concentration of 19 μ L is added is 0.75M's CaSO4。
N is added when preparing cohesive hydrogel by the present invention, and N'- methylene-bisacrylamide is as crosslinking agent, in tetramethyl In the redox initiation system that ethylenediamine, ammonium persulfate form, make acrylamide that Raolical polymerizable occur, forms poly- third Acrylamide, the hydroxyl activated in sodium alginate can form amido bond with polyacrylamide, the power of aquagel can be enhanced Performance is learned, its rapid shaping during the preparation process is made.In addition to this, CaSO4In calcium ion and sodium alginate soln in sea Ion coupling occurs for alginic acid, forms cross-linked network, can also make sticky crystal glue material rapid shaping in a mold.
The present invention also provides a kind of cohesive hydrogel materials, are made according to above-mentioned preparation method.
The cohesive hydrogel is porous structure and no cytotoxicity, is conducive to nerve trachea ectendotrophy mass exchange, thin Born of the same parents' migration and regeneration, have stronger histocompatbility.
Exempt to suture artificial nerve catheter the present invention also provides one kind, including chitosan artificial nerve catheter and bonds respectively Cohesive hydrogel in chitosan artificial nerve catheter both ends end, the sticky water of the cohesive hydrogel by mentioned earlier It is formed after gel rubber material plastic.
The present invention also provides a kind of above-mentioned preparation methods for exempting to suture artificial nerve catheter, comprising the following steps:
(1) chitosan artificial nerve catheter is put into mold, the both mold ends reserve the vacant sky of 1~2cm length Between；
(2) it is artificial to be injected into the chitosan placed in the mold before plastic for the cohesive hydrogel material The both ends of nerve trachea, placement 12~for 24 hours, after the cohesive hydrogel material nature plastic, mold is laid down, must exempt to suture people Work nerve trachea.
Preferably, the mold is matched with the chitosan artificial nerve catheter internal-and external diameter.
Preferably, the length of the chitosan artificial nerve catheter is 5mm.
Exempt to suture artificial nerve catheter the present invention also provides one kind, which is characterized in that by of any of claims 1 or 2 The both ends that sticky porous aquagel is bonded in chitosan artificial nerve catheter respectively are made.
Compared with prior art, in the preparation method of cohesive hydrogel disclosed by the invention, by chitosan, sodium alginate, After acrylamide mixing, it is mixed that 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride, n-hydroxysuccinimide is added N, N'- methylene-bisacrylamide, tetramethylethylenediamine, ammonium persulfate and CaSO are added after even4, obtain cohesive hydrogel material Material, the cohesive hydrogel are porous structure, and no cytotoxicity, good biocompatibility are suitble to implant.In addition, sticky water 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride, the N- hydroxysuccinimidyl acyl being added in gel rubber material preparation process After imines makes the activated carboxylic in sodium alginate, amido bond can be formed with amino in chitosan and tissue, form crosslinking Network.Therefore, which is injected into the mold for being placed with chitosan artificial nerve catheter before plastic End positions, the hydroxyl in sodium alginate is by forming amido bond with chitosan, in chitosan nerves end of conduit position strength Adhesion-molded, preparation, which is formed, exempts to suture artificial nerve catheter.This exempts to suture artificial nerve catheter in use, for example neural When operative catheter is implanted into, enable cohesive hydrogel by forming amido bond with the amino in nerve fiber to strongly adherent mind It is organized, achievees the effect that remove operation stitching from, it is easy to use.
Specific embodiment
For a further understanding of the present invention, the preferred embodiment of the invention is described below with reference to embodiment, still It should be appreciated that these descriptions are intended merely to further illustrate the features and advantages of the present invention, rather than to the claims in the present invention Limitation.
All raw materials of the present invention, are not particularly limited its source, buying on the market or according to those skilled in the art The preparation of conventional method known to member.
Embodiment 1
1) the MES buffer for preparing 1L, adjusts pH to 5.
2) 20g chitosan, 20g sodium alginate and 120g acrylamide are weighed, is dissolved in the MES buffer that configuration obtains, Obtain mixed solution.
3) 120g amino crosslinker 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide hydrochloride is added into mixed solution The n-hydroxysuccinimide (NHS) of salt (EDC), 120g, the concentration that 3.6mL is added after mixing is the covalent crosslinking agent of 20g/L N, N'- methylene-bisacrylamide (MBAA), the accelerator tetramethylethylenediamine (TEMED) of 0.8mL, the concentration of 22.6mL are The concentration of 0.27M initiator ammonium persulfate (APS) and 19.1mL are 0.75M ion crosslinking agent CaSO4, it is uniformly mixed, is glued Property hydrogel material.
5) chitosan artificial nerve catheter is put into the matched plastic mould of internal-and external diameter, it is vacant that both ends reserve 2cm.
6) it is injected into chitosan catheter both ends spare space before the non-plastic of cohesive hydrogel material, places 12h, Natural plastic.
7) mold is laid down, foam washing in distilled water is put into, removes uncrosslinked crosslinking agent and acrylamide.
Embodiment 2
1) the MES buffer for preparing 1L, adjusts pH to 5.
2) 30g chitosan, 30g sodium alginate and 120g acrylamide are weighed, is dissolved in the MES buffer that configuration obtains, Obtain mixed solution.
3) 120g amino crosslinker 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide hydrochloride is added into mixed solution The n-hydroxysuccinimide (NHS) of salt (EDC), 120g, the concentration that 3.6mL is added after mixing is the covalent crosslinking agent of 20g/L N, N'- methylene-bisacrylamide (MBAA), the accelerator tetramethylethylenediamine (TEMED) of 0.8mL, the concentration of 22.6mL are The concentration of 0.27M initiator ammonium persulfate (APS) and 19.1mL are 0.75M ion crosslinking agent CaSO4, it is uniformly mixed, is glued Property hydrogel material.
5) chitosan artificial nerve catheter is put into the matched plastic mould of internal-and external diameter, it is vacant that both ends reserve 2cm.
6) be injected into chitosan catheter both ends spare space before the non-plastic of hydrogel, place 18h, it is natural at Glue.
7) mold is laid down, foam washing in distilled water is put into, removes uncrosslinked crosslinking agent and acrylamide.
Embodiment 3
1) the MES buffer for preparing 1L, adjusts pH to 5.
2) 10g chitosan, 10g sodium alginate and 70g acrylamide are weighed, is dissolved in the MES buffer that configuration obtains, obtains To mixed solution.
3) 120g amino crosslinker 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide hydrochloride is added into mixed solution The n-hydroxysuccinimide (NHS) of salt (EDC), 120g, the concentration that 3.6mL is added after mixing is the covalent crosslinking agent of 20g/L N, N'- methylene-bisacrylamide (MBAA), the accelerator tetramethylethylenediamine (TEMED) of 0.8mL, the concentration of 22.6mL are The concentration of 0.27M initiator ammonium persulfate (APS) and 19.1mL are 0.75M ion crosslinking agent CaSO4, it is uniformly mixed, is glued Property hydrogel material.
5) chitosan artificial nerve catheter is put into the matched plastic mould of internal-and external diameter, it is vacant that both ends reserve 2cm.
6) be injected into chitosan catheter both ends spare space before the non-plastic of hydrogel, place for 24 hours, it is natural at Glue.
7) mold is laid down, foam washing in distilled water is put into, removes uncrosslinked crosslinking agent and acrylamide.
Comparative example 1
1) the MES buffer for preparing 1L, adjusts pH to 5.
2) 20g chitosan, 20g sodium alginate and 120g acrylamide are weighed, is dissolved in the MES buffer that configuration obtains, Obtain mixed solution.
3) 120g amino crosslinker 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide hydrochloride is added into mixed solution The accelerator tetramethylethylenediamine of 0.8mL is added in the n-hydroxysuccinimide (NHS) of salt (EDC), 120g after mixing (TEMED), it is 0.75M ion crosslinking agent that the concentration of 22.6mL, which is the concentration of 0.27M initiator ammonium persulfate (APS) and 19.1mL, CaSO4, it is uniformly mixed, obtains cohesive hydrogel material.
5) chitosan artificial nerve catheter is put into the matched plastic mould of internal-and external diameter, it is vacant that both ends reserve 2cm.
6) it is injected into chitosan catheter both ends spare space before the non-plastic of cohesive hydrogel material, places 18h, Natural plastic.
7) mold is laid down, foam washing in distilled water is put into, removes uncrosslinked crosslinking agent and acrylamide.
Comparative example 2
1) the MES buffer for preparing 1L, adjusts pH to 5.
2) 20g chitosan, 20g sodium alginate and 120g acrylamide are weighed, is dissolved in the MES buffer that configuration obtains, Obtain mixed solution.
3) 120g amino crosslinker 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide hydrochloride is added into mixed solution The n-hydroxysuccinimide (NHS) of salt (EDC), 120g, the concentration that 3.6mL is added after mixing is the covalent crosslinking agent of 20g/L The concentration of N, N'- methylene-bisacrylamide (MBAA), the accelerator tetramethylethylenediamine (TEMED) of 0.8mL and 19.1mL is 0.75M ion crosslinking agent CaSO4, it is uniformly mixed, obtains cohesive hydrogel material.
5) chitosan artificial nerve catheter is put into the matched plastic mould of internal-and external diameter, it is vacant that both ends reserve 2cm.
6) it is injected into chitosan catheter both ends spare space before the non-plastic of cohesive hydrogel material, places 40h, Natural plastic.
7) mold is laid down, foam washing in distilled water is put into, removes uncrosslinked crosslinking agent and acrylamide.
Test case: cohesive hydrogel bonding force test
Exempt to suture the viscous of artificial nerve catheter both ends for what rat sciatic nerve filled in that 1-3 of the embodiment of the present invention is prepared In property hydrogel at 1mm, 10min is fixed under 37 DEG C of wet conditions using surgical clamp.Use omnipotent mechanics tester (TFW- 58, Shanghai Tuofeng Instrument Technology Co.Ltd., China) is with the crosshead of 0.5mm/min Speed is tested, and test result is as shown in table 1.
Adhesion strength of 1 artificial nerve catheter of table to nerve
It is led according to 1 test result of table it can be found that exempting from suture artificial neuron provided by the embodiment of the present invention and comparative example The cohesive hydrogel at pipe both ends is to the adhesion strength of nerve trachea between 0.01~0.1MPa, and wherein (concentration is poly- for shell for embodiment 1 Sugar, 20g/L；Sodium alginate, 20g/L；Acrylamide, 120g/L) exempting from of being prepared suture the viscous of artificial nerve catheter both ends Property hydrogel is most strong to neural adhesion power, can reach 0.1MPa.In addition, caning be found that removal tetramethylethylenediamine according to comparative example (TEMED) it will lead to sticky porous aquagel bonding force decline, removal ammonium persulfate (APS) will not make cohesive hydrogel substantially Bonding force decline, but will lead to gelation time extension.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, for this field skill For art personnel, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from spirit of the invention or In the case where essential characteristic, the present invention can be realized in other specific forms.Therefore, in all respects, should all incite somebody to action Embodiment regards exemplary as, and is non-limiting, the scope of the present invention by appended claims rather than on state Bright restriction, it is intended that including all changes that fall within the meaning and scope of the equivalent elements of the claims in the present invention It is interior.Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, it is possible to understand that A variety of change, modification, replacement and modification can be carried out to these embodiments in the case where not departing from the principle and spirit of the invention, The scope of the present invention is defined by the appended.

Claims (10)

1. a kind of preparation method of cohesive hydrogel material characterized by comprising
1) chitosan, sodium alginate and acrylamide are dissolved in MES buffer, obtain mixed solution；
2) 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride and N- hydroxysuccinimidyl are added in Xiang Suoshu mixed solution Acid imide after being uniformly mixed, adds N, N'- methylene-bisacrylamide, tetramethylethylenediamine, ammonium persulfate and CaSO4, 0.5~1h is stirred at room temperature, obtains cohesive hydrogel material.
2. the preparation method of cohesive hydrogel material according to claim 1, which is characterized in that the MES buffer PH value is 5.
3. the preparation method of cohesive hydrogel material according to claim 1, which is characterized in that each in the mixed solution Constituent content are as follows:
4. the preparation method of cohesive hydrogel material according to claim 1, which is characterized in that each in the mixed solution Constituent content are as follows:
5. the preparation method of cohesive hydrogel material according to claim 1, which is characterized in that 1- (3- bis- in step (2) Methylaminopropyl) -3- ethyl-carbodiimide hydrochloride usage amount be every 1mL the mixed solution in 0.12g is added；N- hydroxyl 0.12g is added in the mixed solution that the usage amount of base succinimide is every 1mL；N, N'- methylene-bisacrylamide The N that 3.6 μ L concentration are 20g/L, N'- methylene-bisacrylamide are added in the mixed solution that usage amount is every 1mL；Tetramethyl The tetramethylethylenediamine of 0.8 μ L is added in the mixed solution that the usage amount of base ethylenediamine is every 1mL；The dosage of ammonium persulfate For be added in the mixed solution of every 1mL 22.6 μ L concentration 0.27M ammonium persulfate；CaSO4Dosage for every 1mL institute State the CaSO for being added that the concentration of 19 μ L is 0.75M in mixed solution4。
6. a kind of cohesive hydrogel material, which is characterized in that according to preparation method system described in Claims 1 to 5 any one .
7. one kind is exempted to suture artificial nerve catheter, which is characterized in that be bonded in institute including chitosan artificial nerve catheter and respectively The cohesive hydrogel of chitosan artificial nerve catheter both ends end is stated, the cohesive hydrogel is by viscosity as claimed in claim 6 It is formed after hydrogel material plastic.
8. exempting from the preparation method of suture artificial nerve catheter as claimed in claim 7, which comprises the following steps:
(1) chitosan artificial nerve catheter is put into mold, the both mold ends reserve the spare space of 1~2cm length；
(2) the cohesive hydrogel material is injected into the chitosan artificial neuron placed in the mold before plastic The both ends of conduit, placement 12~for 24 hours, after the cohesive hydrogel material nature plastic, mold is laid down, must exempt to suture artificial mind Through conduit.
9. the preparation method according to claim 8 for exempting to suture artificial nerve catheter, which is characterized in that the mold and institute State the matching of chitosan artificial nerve catheter internal-and external diameter.
10. the preparation method according to claim 8 for exempting to suture artificial nerve catheter, which is characterized in that the chitosan The length of artificial nerve catheter is 5mm.
CN201910874185.1A 2019-09-17 2019-09-17 Adhesive hydrogel material, suture-free artificial nerve conduit and preparation method thereof Active CN110448727B (en)

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CN115335092B (en) * 2020-01-23 2023-10-31 高丽大学校产学协力团 Nerve suturing patch with self-repairing capability and preparation method thereof
CN111450318A (en) * 2020-03-26 2020-07-28 南通大学 Chitosan nerve conduit with illumination bridged nerve tissue and preparation method and application thereof

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