CN110448688A - A kind of lysozyme formulation, preparation method and application - Google Patents
A kind of lysozyme formulation, preparation method and application Download PDFInfo
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- CN110448688A CN110448688A CN201910856394.3A CN201910856394A CN110448688A CN 110448688 A CN110448688 A CN 110448688A CN 201910856394 A CN201910856394 A CN 201910856394A CN 110448688 A CN110448688 A CN 110448688A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/235—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids having an aromatic ring attached to a carboxyl group
- A61K31/24—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids having an aromatic ring attached to a carboxyl group having an amino or nitro group
- A61K31/245—Amino benzoic acid types, e.g. procaine, novocaine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/006—Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
Abstract
The present invention relates to lysozyme medical fields, specially a kind of lysozyme formulation, preparation method and application, pass through radish lysozyme, gelatin, sodium carboxymethylcellulose, the mixing of the raw materials such as polyvinyl alcohol and peppermint oil, canker sore can effectively be treated, and the effect for treating canker sore has greatly improved compared with Domiphen lozenge, ulcer wound can be made to heal faster than Domiphen lozenge, by the way that mixed liquor to be tuned into neither too hard, nor too soft colloid agent, when atoleine touches the moisture content in oral cavity, it can expand rapidly, it is adsorbed on and suffers from area, adhesion strength is strong, to extend the residence time of drug in the oral cavity, improve curative effect, ulcer wound can be made to heal faster than Domiphen lozenge, and it is approached with the therapeutic effect for having sticking liquid preparation, but the dosage of colloidal lysozyme formulation reduces, the cost for the treatment of can be saved.
Description
Technical field
The present invention relates to lysozyme medical field, specially a kind of lysozyme formulation, preparation method and application.
Background technique
Lysozyme is also known as muramidase or N-acetylmuramide lycanohydrlase, is that a kind of can hydrolyze sticks polysaccharide in pathogenic bacteria
Alkaline enzyme.β-Isosorbide-5-Nitrae glycosidic bond between main -acetylmuramic acid and n-acetylglucosamine by destroying in cell wall,
So that the insoluble glutinous polysaccharide of cell wall is resolved into soluble glycopeptide, cell wall rupture content is caused to escape and make bacterolysis.
Radish lyase bacterium is that a kind of acid resistance heat resistance extracted from Cruciferae radish leaves is superior to the lyase of HEWL
Bacterium, to 3 kinds of G such as staphylococcus albus+Bacterium, 6 kinds of G such as Escherichia coli-5 kinds of fungies such as bacterium and yeast, Mucor are different
The inhibiting effect of degree, and low raw-material cost.
Canker sore is commonly called as " aphtha ", is a kind of common ulcerative damages illness for betiding mucous membrane of mouth, is more common in
The positions such as lip inside, tongue, tongue abdomen, cheek mucous membrane, vestibular ditch, soft palate, the mucous membrane at these positions lack keratinization layer or angling compared with
Difference.Tongue ulcer refers to the canker sore for betiding tongue, tongue abdomen position.Sharp ache when canker sore is broken out, local cusalgia are bright
Aobvious, serious person also will affect diet, speak, and cause very big inconvenience to daily life；It can concurrent halitosis, chronic pharyngitis, constipation, head
Bitterly, the constitutional symptoms such as dizzy, nauseous, out of strength, irritated, fever, enlargement of lymph nodes；Canker sore is typically all to pass through mouth containing at present
The lozenges such as Domiphen lozenge or chlorhexidine lozenge can also dissipate deposited Bingpeng San and water melon frost and dissipate etc..
Above-mentioned existing therapeutic scheme, in treatment, there is some defects, such as lozenge, the residence time is short in oral cavity, makes
The shortcomings that short with duration of efficacy, the time for eventually leading to treatment is long, increases the pain of patient, powder be difficult to after administration it is fixed
Position.
Summary of the invention
The purpose of the present invention is to provide a kind of lysozyme formulations, preparation method and application, to solve the above problems.
To achieve the above object, the invention provides the following technical scheme:
The present invention provides a kind of lysozyme formulation, the effective component of lysozyme formulation includes:
Preferably, the effective component of lysozyme formulation also includes:
A kind of preparation method of lysozyme formulation, method includes the following steps:
1) preparation of radish lysozyme takes mature fresh turnip leaves to shred, 1-1.5 times of material is added
The sodium phosphate buffer of 0.066mol/L, pH6.2, bruisher homogenate, are filtered by the nylon cloths of 140 mesh, by filtrate in
4000r/min is centrifuged 10min, collects supernatant to get crude enzyme liquid, 210ml crude enzyme liquid is mixed simultaneously at 4 DEG C with auxiliary agent
It is slowly stirred 2h, is packed into chromatographic column, is eluted with 0.1mol/L HAc, fraction collection enzymatic activity part, then by it to buffer
Dialysis obtains the radish lysozyme of purification；
2) by 10-30 parts of gelatin, 15-30 parts of sodium carboxymethylcellulose, 10-15 parts of polyvinyl alcohol, first nitre file 5-15 parts, it is general
Cacaine 0.1-0.2 parts and borneol 0.5-2 parts of Shandong is ground into fine powder, is sieved by the sieve of 200 mesh, is pressing peppermint oil 0.5-2 parts
And 25-40 parts of the radish lysozyme produced by step 1) is added in fine powder, and is adequately uniformly mixed by blender, is made
At the sticking liquid preparation of tool.
It preferably, further include by 17-23 parts of honeysuckle, 17-23 parts of cortex cinnamomi, 10-20 parts of west safflower, mountain in step 2)
8-10 parts and Radix Astragali 8-10 parts of medicine are worn into fine powder, are mixed together.
Preferably, in step 1), the auxiliary agent is sufficiently to be balanced, blotted with 0.066mol/L sodium phosphate buffer
Crab shell deamination chitin 25mg.
Preferably, will have sticking liquid preparation and be added atoleine 20-35 parts, mixed liquor will be tuned into neither too hard, nor too soft
Colloid agent when atoleine touches the moisture content in oral cavity, can expand rapidly, be adsorbed on and suffer from area, and adhesion strength is strong, to extend
The residence time of drug in the oral cavity improves curative effect.
A kind of application of lysozyme formulation, including two kinds of application methods,
One is the sticking liquid preparation of tool that is made prepared by the above method to be filled in atomization spray bottle, to oral cavity
Inside is sprayed；Liquid formulation can be uniformly sprayed at cavity interior, can treat canker sore when having canker sore,
When not suffering from canker sore, when being sprayed at cavity interior, the herbal medicine such as honeysuckle, cortex cinnamomi, west safflower, Chinese yam and Radix Astragali can be strong
Strong oral cavity inner membrance prevents canker sore, main application and prevention.
The neither too hard, nor too soft colloid agent prepared by the above method is directly applied to canker sore by another kind, can be incited somebody to action
Colloid agent focuses at canker sore, and tongue licks colloid agent in order to prevent, can also be after coating colloid agent, at it
Stick one layer of water-separating film in surface.
Compared with prior art, the beneficial effects of the present invention are: a kind of lysozyme formulation of the present invention, preparation method
And application, pass through the mixing of the raw materials such as radish lysozyme, gelatin, sodium carboxymethylcellulose, polyvinyl alcohol and peppermint oil, Neng Gouyou
The treatment canker sore of effect, and the effect for treating canker sore has greatly improved compared with Domiphen lozenge, can contain than Domiphen
Piece makes ulcer wound heal faster, and by the way that mixed liquor to be tuned into neither too hard, nor too soft colloid agent, atoleine touches oral cavity
In moisture content when, can expand rapidly, be adsorbed on and suffer from area, adhesion strength is strong, to extend the residence time of drug in the oral cavity, improves
Curative effect can make ulcer wound heal faster than Domiphen lozenge, and connect with the therapeutic effect for having sticking liquid preparation
Closely, but the dosage of colloidal lysozyme formulation reduces, and can save the cost for the treatment of.
Specific embodiment
It is clearly and completely described below in conjunction with the technical solution in the embodiment of the present invention, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without making creative work belongs to what the present invention protected
Range.
Embodiment 1
1) preparation of radish lysozyme takes mature fresh turnip leaves to shred, the 0.066mol/ of 1 times of material is added
The sodium phosphate buffer of L, pH6.2, bruisher homogenate are filtered by the nylon cloth of 140 mesh, and filtrate is centrifuged in 4000r/min
10min collects supernatant to get crude enzyme liquid, sufficiently balanced with 0.066mol/L sodium phosphate buffer, the crab shell deamination that blots it is several
Fourth matter 25mg mixes at 4 DEG C with 210ml crude enzyme liquid and is slowly stirred 2h, is packed into chromatographic column, is eluted with 0.1mol/L HAc, point
Enzymatic activity part is collected in portion, then it obtains buffer dialysis to the radish lysozyme of purification；
2) 20 parts of gelatin, 20 parts of sodium carboxymethylcellulose, 15 parts of polyvinyl alcohol, first nitre are filed 5 parts, 0.1 part of procaine
It is ground into fine powder with 2 parts of borneol, is sieved by the sieves of 200 mesh, it is molten in the radish produced by 2 parts of peppermint oil and by step 1)
40 parts of bacterium enzyme are added in fine powder, and are adequately uniformly mixed by blender, and the sticking liquid preparation of tool is made, will have
The liquid preparation of viscosity is filled in atomization spray bottle, is sprayed to cavity interior.
Look for canker sore patient similar in 10 canker sore degree, to wherein 4 canker sore patient cavity interiors into
Row is spraying, and Domiphen lozenge is contained in mouth as experimental group Isosorbide-5-Nitrae name canker sore patient, as experimental group 2, is left 2 masterpieces
For control group, observation is primary per hour, and through the time of oral cavity ulcers wound healing, the results are shown in Table 1:
Table 1
As shown in Table 1, lysozyme formulation of the present invention can effectively treat canker sore, and treat canker sore
Effect have greatly improved compared with Domiphen lozenge, ulcer wound can be made to heal faster than Domiphen lozenge.
Embodiment 2
1) preparation of radish lysozyme takes mature fresh turnip leaves to shred, 1-1.5 times of material is added
The sodium phosphate buffer of 0.066mol/L, pH6.2, bruisher homogenate, are filtered by the nylon cloths of 140 mesh, by filtrate in
4000r/min is centrifuged 10min, collects supernatant to get crude enzyme liquid, is sufficiently balanced, inhaled with 0.066mol/L sodium phosphate buffer
Dry crab shell deamination chitin 25mg mixes at 4 DEG C with 210ml crude enzyme liquid and is slowly stirred 2h, is packed into chromatographic column, uses
Then it is obtained the radish bacteriolyze of purification by 0.1mol/L HAc elution, fraction collection enzymatic activity part to buffer dialysis
Enzyme；
2) 20 parts of gelatin, 20 parts of sodium carboxymethylcellulose, 15 parts of polyvinyl alcohol, first nitre will be filed 5 parts, procaine 0.1
Part, 2 parts of borneol, 23 parts of honeysuckle, 23 parts of cortex cinnamomi, 20 parts of west safflower, 10 parts of Chinese yam and 10 parts of Radix Astragali wear into fine powder, pass through 200
The screening of purpose sieve, is being added to fine powder by 25-40 parts of 0.5-2 parts of peppermint oil and the radish lysozyme produced by step 1)
In, and be adequately uniformly mixed by blender, the sticking liquid preparation of tool is made, it is filling will to have sticking liquid preparation
Into atomization spray bottle, cavity interior is sprayed.
Look for canker sore patient similar in 10 canker sore degree, to wherein 4 canker sore patient cavity interiors into
Row is spraying, and Domiphen lozenge is contained in mouth as experimental group Isosorbide-5-Nitrae name canker sore patient, as experimental group 2, is left 2 masterpieces
For control group, observation is primary per hour, and through the time of oral cavity ulcers wound healing, the results are shown in Table 2:
Table 2
As shown in Table 2, lysozyme formulation of the present invention can effectively treat canker sore, and treat canker sore
Effect have greatly improved compared with Domiphen lozenge, ulcer wound can be made to heal faster than Domiphen lozenge, and than implement
The therapeutic effect of lysozyme formulation has further promotion in example 1.
Embodiment 3
1) preparation of radish lysozyme takes mature fresh turnip leaves to shred, 1-1.5 times of material is added
The sodium phosphate buffer of 0.066mol/L, pH6.2, bruisher homogenate, are filtered by the nylon cloths of 140 mesh, by filtrate in
4000r/min is centrifuged 10min, collects supernatant to get crude enzyme liquid, is sufficiently balanced, inhaled with 0.066mol/L sodium phosphate buffer
Dry crab shell deamination chitin 25mg mixes at 4 DEG C with 210ml crude enzyme liquid and is slowly stirred 2h, is packed into chromatographic column, uses
Then it is obtained the radish bacteriolyze of purification by 0.1mol/L HAc elution, fraction collection enzymatic activity part to buffer dialysis
Enzyme；To have sticking liquid preparation and be added atoleine 20-35 parts, mixed liquor is tuned into neither too hard, nor too soft colloid agent.
2) by 10-30 parts of gelatin, 15-30 parts of sodium carboxymethylcellulose, 10-15 parts of polyvinyl alcohol, first nitre file 5-15 parts, it is general
Cacaine 0.1-0.2 parts of Shandong, 0.5-2 parts of borneol, 17-23 parts of honeysuckle, 17-23 parts of cortex cinnamomi, 10-20 parts of west safflower, Chinese yam 8-10
Part wears into fine powder with Radix Astragali 8-10 parts, is sieved by the sieve of 200 mesh, is produced by 0.5-2 parts of peppermint oil and by step 1)
25-40 parts of radish lysozyme be added in fine powder, and be adequately uniformly mixed by blender, the sticking liquid of tool be made
Preparation will have sticking liquid preparation and be added atoleine 20-35 parts, mixed liquor is tuned into neither too hard, nor too soft colloid agent,
Neither too hard, nor too soft colloid agent is fitted into filling, colloid need to be only applied at canker sore when in use.It 1) will be by weighing
The sticking liquid preparation of tool that is made that benefit requires any one method described in 3-5 to prepare is filled in atomization spray bottle, counterpart
Intracavitary portion is sprayed；
Canker sore patient similar in 10 canker sore degree is looked for, colloid is smeared into 4 canker sore patients wherein
Canker sore at, Domiphen lozenge is contained in mouth as experimental group Isosorbide-5-Nitrae name canker sore patient, as experimental group 2, is left
2 are as a control group, and observation is primary per hour, and through the time of oral cavity ulcers wound healing, the results are shown in Table 3:
Table 3
As shown in Table 3, lysozyme formulation of the present invention can effectively treat canker sore, and treat canker sore
Effect have greatly improved compared with Domiphen lozenge, ulcer wound can be made to heal faster than Domiphen lozenge, and than implement
The therapeutic effect of lysozyme formulation is close in example 1, but the dosage of lysozyme formulation reduces, and can save the cost for the treatment of.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of lysozyme formulation, which is characterized in that the effective component of lysozyme formulation includes:
2. a kind of lysozyme formulation according to claim 1, which is characterized in that the effective component of lysozyme formulation also includes
Have:
3. a kind of preparation method of lysozyme formulation, which is characterized in that method includes the following steps:
1) preparation of radish lysozyme takes mature fresh turnip leaves to shred, the 0.066mol/ of 1-1.5 times of material is added
The sodium phosphate buffer of L, pH6.2, bruisher homogenate are filtered by the nylon cloth of 140 mesh, and filtrate is centrifuged in 4000r/min
10min collects supernatant to get crude enzyme liquid, mixes and be slowly stirred 2h with auxiliary agent at 4 DEG C for 210ml crude enzyme liquid, fills
Enter chromatographic column, eluted with 0.1mol/L HAc, fraction collection enzymatic activity part, it is then obtained into purification to buffer dialysis
Radish lysozyme；
2) 10-30 parts of gelatin, 15-30 parts of sodium carboxymethylcellulose, 10-15 parts of polyvinyl alcohol, first nitre are filed into 5-15 parts, Proca
Because 0.1-0.2 parts and borneol 0.5-2 part are ground into fine powder, sieved by the sieve of 200 mesh, press peppermint oil 0.5-2 parts and
25-40 parts of the radish lysozyme produced by step 1) is added in fine powder, and is adequately uniformly mixed by blender, and tool is made
Sticking liquid preparation.
4. a kind of preparation method of lysozyme formulation according to claim 3, it is characterised in that: in step 2), also wrap
It includes and 17-23 parts of honeysuckle, 17-23 parts of cortex cinnamomi, 10-20 parts of west safflower, 8-10 parts of Chinese yam and 8-10 parts of Radix Astragali is worn into fine powder, one
And it is mixed.
5. a kind of preparation method of lysozyme formulation according to claim 3, it is characterised in that: described in step 1)
Auxiliary agent is the crab shell deamination chitin 25mg for sufficiently being balanced, being blotted with 0.066mol/L sodium phosphate buffer.
6. a kind of preparation method of lysozyme formulation according to claim 3, it is characterised in that: will have sticking liquid
Preparation is added atoleine 20-35 parts, and mixed liquor is tuned into neither too hard, nor too soft colloid agent.
7. a kind of application of lysozyme formulation, it is characterised in that: including two kinds of application methods,
1) the sticking liquid preparation of tool that is made of the preparation of any one method described in claim 3-5 is filled to atomization
In spray bottle, cavity interior is sprayed；
2) canker sore directly will be applied to by the neither too hard, nor too soft colloid agent of method preparation as claimed in claim 6.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257732A (en) * | 1999-12-22 | 2000-06-28 | 余美旺 | Chinese patent medicine 'Suxiaokouchuangling capsule' for treating oral ulcer and Baiser's disease |
| CN1745846A (en) * | 2005-09-14 | 2006-03-15 | 夏运岳 | Dental ulcerative glue |
| CN104257636A (en) * | 2014-09-26 | 2015-01-07 | 天津市聚星康华医药科技有限公司 | Lysozyme oral mucoadhesive films and preparation method thereof |
-
2019
- 2019-09-11 CN CN201910856394.3A patent/CN110448688A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257732A (en) * | 1999-12-22 | 2000-06-28 | 余美旺 | Chinese patent medicine 'Suxiaokouchuangling capsule' for treating oral ulcer and Baiser's disease |
| CN1745846A (en) * | 2005-09-14 | 2006-03-15 | 夏运岳 | Dental ulcerative glue |
| CN104257636A (en) * | 2014-09-26 | 2015-01-07 | 天津市聚星康华医药科技有限公司 | Lysozyme oral mucoadhesive films and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 付文艳等: "药物治疗口腔溃疡", 《中国医药指南》 * |
| 卢顺舵，徐凤彩，李明启: "应用亲和层析制备萝卜叶溶菌酶", 《华南农业大学学报》 * |
| 张石革等: "咽喉炎与药物治疗", 《中国药房》 * |
| 高向阳等: "萝卜溶菌酶抑菌效果的初步研究", 《华南农业大学学报》 * |
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Application publication date: 20191115 |