CN110447904A - A kind of peptide activity substance of purification - Google Patents
A kind of peptide activity substance of purification Download PDFInfo
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- CN110447904A CN110447904A CN201810432433.2A CN201810432433A CN110447904A CN 110447904 A CN110447904 A CN 110447904A CN 201810432433 A CN201810432433 A CN 201810432433A CN 110447904 A CN110447904 A CN 110447904A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 100
- 239000000126 substance Substances 0.000 title claims abstract description 42
- 230000000694 effects Effects 0.000 title claims abstract description 37
- 238000000746 purification Methods 0.000 title claims abstract description 27
- 241000233805 Phoenix Species 0.000 claims abstract description 17
- 241000208340 Araliaceae Species 0.000 claims abstract description 16
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 16
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 16
- 235000008434 ginseng Nutrition 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 13
- 239000004475 Arginine Substances 0.000 claims abstract description 12
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000013325 dietary fiber Nutrition 0.000 claims abstract description 12
- AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 claims abstract description 12
- 235000019136 lipoic acid Nutrition 0.000 claims abstract description 12
- 229960002663 thioctic acid Drugs 0.000 claims abstract description 12
- 241000190633 Cordyceps Species 0.000 claims abstract description 10
- 102000008186 Collagen Human genes 0.000 claims abstract description 9
- 108010035532 Collagen Proteins 0.000 claims abstract description 9
- 244000241838 Lycium barbarum Species 0.000 claims abstract description 9
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 9
- 235000015468 Lycium chinense Nutrition 0.000 claims abstract description 9
- 229920001436 collagen Polymers 0.000 claims abstract description 9
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 9
- 239000004615 ingredient Substances 0.000 claims abstract description 9
- 239000003292 glue Substances 0.000 claims abstract description 8
- 239000004833 fish glue Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 4
- 230000001376 precipitating effect Effects 0.000 claims description 4
- 239000013049 sediment Substances 0.000 claims description 4
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000013618 particulate matter Substances 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 241000251468 Actinopterygii Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 29
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- 238000005868 electrolysis reaction Methods 0.000 abstract description 4
- 235000019658 bitter taste Nutrition 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000003513 alkali Substances 0.000 abstract description 2
- 150000004703 alkoxides Chemical class 0.000 abstract description 2
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 abstract description 2
- 230000008827 biological function Effects 0.000 abstract description 2
- 230000001228 trophic effect Effects 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 description 8
- 230000004151 fermentation Effects 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000010291 electrical method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 206010002953 Aphonia Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 208000012886 Vertigo Diseases 0.000 description 1
- 239000003377 acid catalyst Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000000991 chicken egg Anatomy 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 231100000889 vertigo Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to a kind of peptide activity substances of purification, its main component is phoenix clothing active peptide, ginseng active peptide, Isin glue collagen peptide and peptide water-soluble dietary fiber, its adjunct ingredient is arginine, Cordyceps, lipoic acid, wolfberry fruit extract, by mass percentage, the main component of the peptide activity substance of purification accounts for 86%-94%, adjunct ingredient accounts for 6%-14%, increase phoenix clothing, ginseng, the stability of the peptide materials such as fish glue, compare acid system, for alkaline process, electrolysis method is mild, environmental protection, production technology is simple, small investment, it is quick, it is suitable for industrialized production.Without chemical acid, the residual of alkali and inorganic alkoxide, the peptide produced is not required to digest direct absorption without bitter taste, peptide molecular weight small (molecular weight is mostly below 1000), these small-molecular peptides, with power, carrier, transport, mediator and trophic function, especially its biological function with extremely strong activity and diversity, that is, important.
Description
Technical field
The invention belongs to field of biotechnology, more particularly to a kind of peptide activity substance of purification.
Background technique
Peptide is the segment of protein structure and function in biology defines, and passes through peptide to multiple amino acid moleculars by two
Chain link composition, and the active gene part that protein plays a role.The intracorporal amino acid of people only has 20 kinds, but different groups
Conjunction just has different peptides, therefore the type of peptide is very more.Peptide occupies the most fundamental position in the seven major nutrient of people.
For peptide compared with other substances of organism, maximum feature is that have extremely strong activity and diversity.Peptide is research
Life source, structure, function source, be the basis of study of disease, physiological characteristic, cell tissue.International bio chemistry, life
Science, medicine sector brainstrust unanimously think: 21st century by be peptide century.Peptide is as Biochemical Engineering, genetic engineering
In important research content, be put into the Chinese government 863 planning among.Peptide is also that international scientific technology is most advanced, competes most
Fierce one of field.There are up to ten thousand scientists to have studied over half a century in world wide, has become high-tech production now
The new hot spot of industry.
Effect of the peptide to human body, scientist are summarised as roughly eight words, that is: inhibit, activate, repair, promote:
Inhibit --- inhibit cell degeneration, enhances human immunological competence；
Activation --- active cell activity effectively removes the free radical being harmful to the human body；
Repair --- human body degeneration of cells are repaired, cell metabolism is improved；
Promote --- promote, maintain cell normally metabolic.
The method that tradition obtains peptide has very much.Traditional Method mainly has: microbe fermentation method, acid system, alkaline process, electrical method, artificial
Grafting, gene expression method, enzymatic isolation method etc..
Microbe fermentation method: the production technology of microbe fermentation method mainly passes through Modern microbiological fermentation technique will
Macromolecular globulin is converted into small-molecular peptides, can produce different aminoacids sequence by the metabolism and fermentation condition that control microorganism
The peptide different with molecular weight.During the fermentation, the free amino acid of generation is absorbed and utilized again by microorganism, to microorganism
Metabolism will not generate feedback inhibition.By the metabolism of microorganism, transposing and rearrangement are carried out to amino acid and small peptide, to certain
Peptide group is modified and is recombinated.Such as using soybean meal as raw material undergo microbial fermentation method production soybean peptide, change
The intrinsic amino acid sequence of soybean protein has modified the hydrophobic amino acid end of peptide, so that soybean peptide is not had bitter taste, activity is more
Height, and assign soybean peptide some bioactive functions, belong to bioengineering new and high technology range, it is high in technological content, in food
It can be applied in the industries such as industry, fermentation industry, feedstuff industry, pharmaceutical industry, cosmetic industry and plant nutrient promotor.Tool
There are very extensive purposes and boundless development and application prospect.
Acid system: with the method for chemical strong acid catalyst protein acquisition peptide acid system, the investment of this method is big, land occupation is more, technique is multiple
It is miscellaneous, pollution is big, molecular weight is difficult to control, product has chemical residual, it is difficult to form function, be difficult to realize industrialized production, so far
Remain in laboratory；
Alkaline process: being called alkaline process with the method for chemical strong base catalyst protein, and this method is reached the same goal by different routes with acid system, the result is that
The same；
Enzyme process: it is known as enzyme process with the method for biological enzyme protein.Enzyme process have breakthrough on the basis of Traditional Method and
Innovation, has adapted to low-carbon economy and environmentally protective requirement.Enzyme process is exactly that biological enzyme protein is used to obtain polypeptide, is exactly egg
White matter degradation, artificial synthesized peptide.Enzyme process is mild, environmentally friendly compared with acid system, alkaline process, electrical method.Production technology is simple, and small investment takes effect
It fastly, is suitable for industrialized production.
Phoenix clothing, after the entitled Phasianidae animal family chicken of Chinese medicine (Gallus gallus domesticus Brisson) ovum incubates chicken
Egg membrane in eggshell.The effect of with nourishiing yin to clear away the lung-heat, sore, disappear screen, synthetism.It pants for chronic cough, pharyngalgia aphonia, lymph node
Core, unhealing ulcer, the raw ocular of mesh, vertigo, trauma fracture.
How this traditional Chinese medicine medicinal material such as phoenix clothing, ginseng, cordyceps sinensis, fructus lycii to be merged with hard-core technology, is carried out
Purification and decomposition, are hot spot concerned by people and focus, and technological gap all the time.
Summary of the invention
The present invention provides a kind of peptide activity substances of purification, it is therefore intended that in conjunction with phoenix clothing, ginseng, cordyceps sinensis, fructus lycii etc.
This traditional Chinese medicine medicinal material is purified, and the stabilization of peptide activity substance is improved.
Technical solution provided by the invention is as follows:
A kind of peptide activity substance of purification, main component are phoenix clothing active peptide, ginseng active peptide, Isin glue collagen peptide
With peptide water-soluble dietary fiber, adjunct ingredient is arginine, Cordyceps, lipoic acid, wolfberry fruit extract, by quality percentage
Than the main component of the peptide activity substance of purification accounts for 86%-94%, and adjunct ingredient accounts for 6%-14%.
In above-mentioned technical proposal, by the gross mass percentage of the peptide activity substance of purification, peptide water-soluble dietary fiber is accounted for
30%-50%, phoenix clothing active peptide, ginseng active peptide, Isin glue collagen peptide account for 44%-56% in total.
In above-mentioned technical proposal, by the gross mass percentage of the peptide activity substance of purification, Cordyceps and fructus lycii are extracted
Object accounts for 2%-6%, and arginine and lipoic acid account for 4%-8%.
As a preferred embodiment of the above technical solution, by the gross mass percentage of the peptide activity substance of purification, main component phoenix
Phoenix clothing active peptide is 40%, ginseng active peptide 5%, Isin glue collagen peptide 9% and peptide water-soluble dietary fiber 40%；Its auxiliary material at
Middle arginine is divided to be 1%, Cordyceps 1%, lipoic acid 3%, wolfberry fruit extract 1%.
As a preferred embodiment of the above technical solution, by the gross mass percentage of the peptide activity substance of purification, main component phoenix
Phoenix clothing active peptide is 30%, ginseng active peptide 10%, Isin glue collagen peptide 6% and peptide water-soluble dietary fiber 40%；Its auxiliary material
In ingredient arginine be 5%, Cordyceps 3%, lipoic acid 3%, wolfberry fruit extract 3%.
Present invention simultaneously provides the processing methods of the peptide activity substance about above-mentioned purification, the steps include:
Q1: it by medicinal material phoenix clothing, ginseng, fish glue, one by one cleaned, dried；
Q2: the medicinal material after cleaning is dried carries out ultraviolet disinfection；
Q3: Q2 step resulting materials crush as coarse powder；
Q4: coarse powder is decocted 2 times in distilled water, then impurity screening；
Q5: it is powered after filtered stoste progress secondary filter and is electrolysed herein, be then allowed to stand and carry out particulate matter precipitating；
Q6: obtained sediment is lyophilized, and obtains crude fibre substance；
Q7: crude fibre substance is dissolved in the distilled water by 5 times of volumes, and peptide water-soluble dietary fiber, arginine, worm is added
Careless extract, lipoic acid, wolfberry fruit extract, precipitating of being sieved after mixing, refilter；
Q8: filtered solution is lyophilized after being precipitated, the peptide activity substance finally purified.
The invention has the advantages that increasing the stability of the peptide materials such as phoenix clothing, ginseng, fish glue, the acid system that compares, alkaline process come
It says, electrolysis method is mild, environmentally friendly.Production technology is simple, small investment, quick, suitable industrialized production.Without chemical acid, alkali and
The residual of inorganic alkoxide, the peptide produced is without bitter taste, peptide molecular weight small (molecular weight is mostly below 1000), these small-molecular peptides
It is not required to digest direct absorption, there is power, carrier, transport, mediator and trophic function, especially it is with extremely strong active and more
The biological function of sample, that is, important.
Detailed description of the invention
Fig. 1 is a kind of step process signal of the processing method of the peptide activity substance of purification provided in an embodiment of the present invention
Figure.
Specific embodiment
It elaborates below to the preferred embodiment of the present invention.
As shown in Figure 1, in preparation process, by medicinal material phoenix clothing, ginseng, fish glue, is one by one cleaned, dried, then
It is crushed after carrying out ultraviolet disinfection, obtains coarse powder；
In primary procedure of processing, coarse powder is dissolved in the distilled water by 10 times of volumes, is decocted 2 times, then with 40 meshes
It is filtered impurity, when impurity screening uses twice of 40 mesh screen；
In further processing step, filtering solution is subjected to energization electrolysis, electrolysis time is about 3-10 minutes, then into
Row stands 30-45 minutes, to guarantee that particulate matter precipitates；
Sediment is lyophilized, crude fibre substance is obtained；
Finally, crude fibre substance is dissolved in getting away in i.e. 100 DEG C or so of distilled water by 5 times of volumes, enter peptide water solubility
Dietary fiber, arginine, Cordyceps, lipoic acid, wolfberry fruit extract cross 80 mesh screens, precipitate, refilter one after mixing
It is secondary；
Sediment is lyophilized, the peptide activity substance finally purified.
Certainly, those skilled in the art are it should be appreciated that above embodiments are intended merely to illustrate the present invention
, and not limitation of the invention, as long as within the scope of the invention to the variation of above embodiments, modification all in the present invention
Protection scope within.
Claims (6)
1. a kind of peptide activity substance of purification, which is characterized in that its main component is phoenix clothing active peptide, ginseng active peptide, fish
Collagen peptide and peptide water-soluble dietary fiber, adjunct ingredient be arginine, Cordyceps, lipoic acid, wolfberry fruit extract,
By mass percentage, the main component of the peptide activity substance of purification accounts for 86%-94%, and adjunct ingredient accounts for 6%-14%.
2. a kind of peptide activity substance of purification according to claim 1, which is characterized in that by the peptide activity substance of purification
Gross mass percentage, peptide water-soluble dietary fiber account for 30%-50%, phoenix clothing active peptide, ginseng active peptide, Isin glue collagen peptide
44%-56% is accounted in total.
3. a kind of peptide activity substance of purification according to claim 1, which is characterized in that by the peptide activity substance of purification
Gross mass percentage, Cordyceps and wolfberry fruit extract account for 2%-6%, and arginine and lipoic acid account for 4%-8%.
4. a kind of peptide activity substance of purification according to claim 1, which is characterized in that by the peptide activity substance of purification
Gross mass percentage, main component phoenix clothing active peptide are 40%, ginseng active peptide 5%, Isin glue collagen peptide 9% and peptide water
Soluble dietary fiber 40%；In its adjunct ingredient arginine be 1%, Cordyceps 1%, lipoic acid 3%, fructus lycii extract
Object is 1%.
5. a kind of peptide activity substance of purification according to claim 1, which is characterized in that by the peptide activity substance of purification
Gross mass percentage, main component phoenix clothing active peptide are 30%, ginseng active peptide 10%, Isin glue collagen peptide 6% and peptide
Water-soluble dietary fiber 40%；Arginine is 5% in its adjunct ingredient, Cordyceps 3%, lipoic acid 3%, fructus lycii mention
Taking object is 3%.
6. a kind of peptide activity substance of purification according to claim 1, which is characterized in that the peptide activity substance of above-mentioned purification
Processing method, the steps include:
Q1: it by medicinal material phoenix clothing, ginseng, fish glue, one by one cleaned, dried；
Q2: the medicinal material after cleaning is dried carries out ultraviolet disinfection；
Q3: Q2 step resulting materials crush as coarse powder；
Q4: coarse powder is decocted 2 times in distilled water, then impurity screening；
Q5: it is powered after filtered stoste progress secondary filter and is electrolysed herein, be then allowed to stand and carry out particulate matter precipitating；
Q6: obtained sediment is lyophilized, and obtains crude fibre substance；
Q7: crude fibre substance is dissolved in the distilled water by 5 times of volumes, and peptide water-soluble dietary fiber, arginine, cordyceps sinensis is added and mentions
Object, lipoic acid, wolfberry fruit extract are taken, precipitating of being sieved after mixing refilters；
Q8: filtered solution is lyophilized after being precipitated, the peptide activity substance finally purified.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810432433.2A CN110447904A (en) | 2018-05-08 | 2018-05-08 | A kind of peptide activity substance of purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810432433.2A CN110447904A (en) | 2018-05-08 | 2018-05-08 | A kind of peptide activity substance of purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110447904A true CN110447904A (en) | 2019-11-15 |
Family
ID=68480380
Family Applications (1)
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|---|---|---|---|
| CN201810432433.2A Pending CN110447904A (en) | 2018-05-08 | 2018-05-08 | A kind of peptide activity substance of purification |
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| Country | Link |
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| CN (1) | CN110447904A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120302731A1 (en) * | 2010-01-14 | 2012-11-29 | South China University Of Technology | Protein hydrolysate, polypeptide solution and polypeptide, preparation method and use thereof |
| US20140363418A1 (en) * | 2013-06-10 | 2014-12-11 | Molecular Biology International, Inc. | Method of producing collagen from hydrolyzed egg membrane |
| CN106083990A (en) * | 2016-06-02 | 2016-11-09 | 安徽新泰药业有限公司 | A kind of sealwort peptide extracting method and sealwort complex peptides powder |
-
2018
- 2018-05-08 CN CN201810432433.2A patent/CN110447904A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120302731A1 (en) * | 2010-01-14 | 2012-11-29 | South China University Of Technology | Protein hydrolysate, polypeptide solution and polypeptide, preparation method and use thereof |
| US20140363418A1 (en) * | 2013-06-10 | 2014-12-11 | Molecular Biology International, Inc. | Method of producing collagen from hydrolyzed egg membrane |
| CN106083990A (en) * | 2016-06-02 | 2016-11-09 | 安徽新泰药业有限公司 | A kind of sealwort peptide extracting method and sealwort complex peptides powder |
Non-Patent Citations (1)
| Title |
|---|
| 南京中医药大学编著: "《中药大辞典 第2版 上》", 30 June 2014, 上海科学技术出版社 * |
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Application publication date: 20191115 |