CN110447634A - A kind of monkey red blood cells save liquid and application - Google Patents
A kind of monkey red blood cells save liquid and application Download PDFInfo
- Publication number
- CN110447634A CN110447634A CN201910568840.0A CN201910568840A CN110447634A CN 110447634 A CN110447634 A CN 110447634A CN 201910568840 A CN201910568840 A CN 201910568840A CN 110447634 A CN110447634 A CN 110447634A
- Authority
- CN
- China
- Prior art keywords
- red blood
- monkey
- blood cells
- liquid
- monkey red
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Abstract
The invention discloses a kind of monkey red blood cells to save liquid and its application.The monkey red blood cells save liquid and are able to maintain the integrality of monkey red blood cells, and can be avoided the growth of the microorganisms such as bacterium.Preservation system includes glucose, Tris-HCL, ATP, mannose, the chief actives reagent such as potassium citrate.Preservation liquid of the invention can enhance the nutrition supply of red blood cell, be protected its cell membrane, and reduce the metabolism etc. of red blood cell, to extend the holding time of red blood cell.The monkey red blood cells of acquisition can be reserved for 6 months, can be applied to the fields such as the serological surveillance of morbilli.
Description
Technical field
The present invention relates to biology techniques fields more particularly to a kind of monkey red blood cells to save liquid and application thereof.
Background technique
Morbilli may be that the mankind's candidate third eliminated after the polio that exterminates smallpox and will eliminate passes
It catches an illness.Generally use measurement serum Measles Antibody Level Among both at home and abroad to understand the immune state of crowd, hemagglutination-inhibition test is to survey
Determine experimental method common, quickly, easy in measles antibody, but the disadvantage is that the test is not only coagulated with fresh monkey red blood cells
Collection inhibit test, also need with a large amount of monkey red blood cells absorb it is to be measured enter serum in nonspecific agglutination substance.However monkey is red thin
Born of the same parents source is few, and is not easy to save for a long time, and the extensive development of hemagglutination-inhibition test is made to be very limited.Therefore one kind is researched and developed to match
Side, enhances the nutrition supply of red blood cell, is protected its cell membrane, and reduces the metabolism etc. of red blood cell, to extend red
The holding time of blood cell.Measles Hemagglutination Inhibition Antibody measurement large-scale promotion is possibly realized by this, promotes the serum school superintendent of morbilli
The development with seroepidemiology work is surveyed, is made contributions for control or even the final work for eliminating morbilli.
Summary of the invention
The object of the present invention is to provide a kind of monkey red blood cells to save liquid and application thereof.The monkey red blood cells save liquid and can protect
The integrality of monkey red blood cell is held, and can be avoided the growth of the microorganisms such as bacterium.
The technical solution adopted by the present invention:
The present invention provides a kind of monkey red blood cells preservation liquid for extending monkey red blood cells storage life, and the preservation liquid is by as follows
The group of proportion is grouped as: glucose 150mmol/L, ammonium chloride
50mmol/L, adenosine triphyosphate (ATP) 3mmol/L, adenine 2mmol/L, mannitol 50mmol/L, lemon
Lemon acid potassium 20mmol/L, dipotassium hydrogen phosphate 25mmol/L, potassium dihydrogen phosphate 20mmol/L, solvent is distilled water, uses sodium hydroxide
It is 7.1 that aqueous solution, which adjusts pH value,.
Save liquid the present invention also provides a kind of monkey red blood cell and saving the application in monkey red blood cell, the application be by
Monkey red blood cell saves liquid with monkey red blood cell and is saved according to volume ratio 1:1 mixing, is able to extend monkey red blood cell in room temperature (25-
30 DEG C) pot-life 6 months.
Compared with prior art, saving liquid the beneficial effects are mainly reflected as follows: monkey red blood cell of the present invention can be
Phenomena such as saving monkey red blood cell integrality under room temperature, haemolysis not occurring.It is experimentally confirmed that being saved using preservation liquid of the invention
Monkey red blood cells rupture does not occur after 6 months for monkey red blood cell.In addition, without the growth of the microorganisms such as bacterium, inhibit convenient for blood clotting etc.
The operation of test.
Detailed description of the invention
The preservation effect figure of the fresh acquisition monkey red blood cells of Fig. 1, the left side are that addition saves liquid, and the right is not add preservation liquid.
Fig. 2 saves the effect picture of 1 month monkey red blood cells, and the left side is that addition saves liquid, and the right is not add preservation liquid.
Fig. 3 saves the effect picture of 2 months monkey red blood cells, and the left side is that addition saves liquid, and the right is not add preservation liquid.
Fig. 4 saves the effect picture of 3 months monkey red blood cells, and the left side is that addition saves liquid, and the right is not add preservation liquid.
Fig. 5 saves the effect picture of 4 months monkey red blood cells, and the left side is that addition saves liquid, and the right is not add preservation liquid.
Fig. 6 saves the effect picture of 5 months monkey red blood cells, and the left side is that addition saves liquid, and the right is not add preservation liquid.
Fig. 7 saves the effect picture of 6 months monkey red blood cells, and the left side is that addition saves liquid, and the right is not add preservation liquid.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
1 monkey red blood cells of embodiment save liquid
Monkey red blood cells save liquid and are made of the raw material matched as follows: glucose 150mmol/L, ammonium chloride 50mmol/L, gland
Purine nucleosides triphosphoric acid (ATP) 3mmol/L, adenine 2mmol/L, mannitol 50mmol/L, potassium citrate 20mmol/L, phosphoric acid
Hydrogen dipotassium 25mmol/L, potassium dihydrogen phosphate 20mmol/L, solvent is distilled water, and adjusting pH value with sodium hydrate aqueous solution is 7.1.
2 monkey red blood cells of embodiment save liquid and are saving the application in monkey red blood cells
1, the acquisition of monkey red blood cells
Rhesus macaque is picked up from, 4 DEG C of preservations in vein sterile blood sampling postposition Alsever's Solution use in one week.
2, morbilli hemagglutination-inhibition test
A) reagent: there is measles haemagglutinin 1 in morbilli HI test kit, serum non-specific adsorption substance 1
Bottle (for absorbing the nonspecific agglutination substance in human serum), test monkey red blood cells and the mixing for saving liquid volume ratio 1:1
1 bottle of liquid.Wherein, mixed liquor is the fresh monkey red blood cells of acquisition, and 1:1 is stored in the preservation liquid of embodiment 1 by volume respectively, In
It saves 1 month, 2 months, 3 months, 4 months, 5 months and 6 months respectively at room temperature.In addition control group is set, monkey red blood cell is straight
It connects and is stored in anti-coagulants.
B) morbilli hemagglutination-inhibition test is carried out to 5 parts of crowd's serum to be measured that laboratory saves, " plan is exempted from referring to the Ministry of Public Health
Epidemic disease technical management regulation " it carries out.Morbilli HI test kit application method is located referring to kit specification in serum in advance
When reason, draws 60 μ L of serum to be checked and be added in 1ml plastics point bottom tube, 56 DEG C of inactivations, 30 minutes add the blood mixed well
Clear 90 μ L of non-specific adsorption substance.After the two is shaked mixing, 4 DEG C are set overnight, next day Aspirate supernatant is 1:2 diluted blood
Clearly, following steps are the same as conventional morbilli hemagglutination-inhibition test.Different time (1 month, 2 months, 3 months, 4 months, 5 months and 6
A month) save sensitive monkey red blood cells corkage after, washed 3 times with physiology salt, it is red that hematocrit monkey red blood cells with physiological saline are made into 1% monkey
Cell suspension carries out hemagglutination-inhibition test, the results are shown in Table 1.
The monkey red blood cells of the different holding times of table 1 are used for morbilli hemagglutination-inhibition test (Measles Hemagglutination Inhibition Antibody titre)
Claims (3)
1. a kind of monkey red blood cells save liquid, it is characterised in that the preservation liquid is grouped as by the group matched as follows: glucose
150mmol/L, ammonium chloride 50mmol/L, adenosine triphyosphate (ATP) 3mmol/L, adenine 2mmol/L, mannitol
50mmol/L, potassium citrate 20mmol/L, dipotassium hydrogen phosphate 25mmol/L, potassium dihydrogen phosphate 20mmol/L, solvent are distilled water,
PH value is 7.1.
2. monkey red blood cell described in a kind of claim 1 saves liquid and is saving the application in monkey red blood cell.
3. application as claimed in claim 2, it is characterised in that the application be monkey red blood cell and monkey red blood cell are saved liquid by
It is saved according to volume ratio 1:1 mixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910568840.0A CN110447634A (en) | 2019-06-27 | 2019-06-27 | A kind of monkey red blood cells save liquid and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910568840.0A CN110447634A (en) | 2019-06-27 | 2019-06-27 | A kind of monkey red blood cells save liquid and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110447634A true CN110447634A (en) | 2019-11-15 |
Family
ID=68481719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910568840.0A Pending CN110447634A (en) | 2019-06-27 | 2019-06-27 | A kind of monkey red blood cells save liquid and application |
Country Status (1)
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| CN (1) | CN110447634A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110907642A (en) * | 2019-12-02 | 2020-03-24 | 青岛农业大学 | Kit and method for rapidly detecting canine parvovirus antigen and canine parvovirus antibody |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4267269A (en) * | 1980-02-05 | 1981-05-12 | Baxter Travenol Laboratories, Inc. | Red cell storage solution |
| EP0313808A2 (en) * | 1987-09-21 | 1989-05-03 | Stein Holme | Synthetic, plasma-free, transfusible storage medium for red blood cells |
| CN1195965A (en) * | 1995-06-05 | 1998-10-14 | 加州大学评议会 | Method using oxygen removal for extending useful shelf-life of refrigerated red blood cells |
| CN106110421A (en) * | 2016-07-01 | 2016-11-16 | 翁炳焕 | Rhesus monkey erythrocytes adsorber |
| CN106561634A (en) * | 2016-11-14 | 2017-04-19 | 北京乐普医疗科技有限责任公司 | Red cell storage solution and preparation method thereof |
-
2019
- 2019-06-27 CN CN201910568840.0A patent/CN110447634A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4267269A (en) * | 1980-02-05 | 1981-05-12 | Baxter Travenol Laboratories, Inc. | Red cell storage solution |
| EP0313808A2 (en) * | 1987-09-21 | 1989-05-03 | Stein Holme | Synthetic, plasma-free, transfusible storage medium for red blood cells |
| CN1195965A (en) * | 1995-06-05 | 1998-10-14 | 加州大学评议会 | Method using oxygen removal for extending useful shelf-life of refrigerated red blood cells |
| CN106110421A (en) * | 2016-07-01 | 2016-11-16 | 翁炳焕 | Rhesus monkey erythrocytes adsorber |
| CN106561634A (en) * | 2016-11-14 | 2017-04-19 | 北京乐普医疗科技有限责任公司 | Red cell storage solution and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 卢亦愚等: "麻疹血凝抑制试验试剂盒的研制与应用", 《中国计划免疫》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110907642A (en) * | 2019-12-02 | 2020-03-24 | 青岛农业大学 | Kit and method for rapidly detecting canine parvovirus antigen and canine parvovirus antibody |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
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Application publication date: 20191115 |