CN110441084A - A kind of frozen section method of jackfruit pulp - Google Patents
A kind of frozen section method of jackfruit pulp Download PDFInfo
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- CN110441084A CN110441084A CN201910831751.0A CN201910831751A CN110441084A CN 110441084 A CN110441084 A CN 110441084A CN 201910831751 A CN201910831751 A CN 201910831751A CN 110441084 A CN110441084 A CN 110441084A
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- 238000002474 experimental method Methods 0.000 abstract description 4
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- Life Sciences & Earth Sciences (AREA)
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The invention discloses a kind of frozen section methods of jackfruit pulp, and including the use of sucrose-FAA fixer fixing process jackfruit pulp, recycling the phosphate buffer of sucrose to embathe is to carry out osmotic protection to fixed sample tissue.It is placed in glycerol, and vacuum suction, is jointly processed by jackfruit Meat Sample using mixing embedding medium after incubator culture, embedded sample is placed in quick-frozen in liquid nitrogen, quick-frozen good sample is placed in household freezer and is risen again, is sliced, dyes.The each step of the invention is closely connected, mutually synergistic effect, existing Frozen sections operating procedure is optimized, reduce experimental procedure and saves experimental period, reduce the generation of jackfruit flesh cell ice crystal, the broken of cell is reduced, the integrality of eucaryotic cell structure and institutional framework is ensure that, clearly pulp organization slice map can be obtained.
Description
Technical field
The present invention relates to the technical field of frozen section more particularly to a kind of frozen section methods of jackfruit pulp.
Background technique
Jackfruit: also known as jackfruit, alias bud trailing plants, jackfruit, artocarpus heterophyllus, big artocarpus heterophyllus, candied winter melon-growth and good health, cows belly fruit, fruit
It is real plump soft, fresh and sweet palatable, aromatic flavour, therefore it is known as " tropical fruit (tree) queen ".It is world-renowned tropical fruit (tree), belongs to mulberry
Section Artocarpus aiphyllium is grown on equator area more, has light stink, mature sarcocarp is yellow, sweet and dilitious, fruit
Tie Yu Shugan, built-in countless golden yellow pork pies contain sugar, fat, protein in pulp, and taste depth sweet tea, plump soft, sweetness can
Mouthful, the fruit of aromatic flavour, pineapple mamoncillo is light yellow, have quench the thirst, promote lactation, effects of tonifying middle-Jiao and qi, the nutritive value of jackfruit
Very high, at the same time, there are also very high medical values for jackfruit.Jackfruit can be generally divided into dry pulp fruit (dry bud) and more berries
Two kinds of (wet bud).The former pulp is thick, and fruit juice is few, fragrant and sweet sharp and clear；The latter's pulp is wet soft, and fruit juice is more, and taste is especially sweet.
Dicing method is mostly the ultra-thin section after paraffin section and embedding at present, manufacturing process and step is all relatively time-consuming takes
Power.And Frozen sections are that one kind makes to have fixed under cryogenic or flesh tissue block is first freezed without dehydration and embedding
It is cooled fast to certain degree of hardness, a kind of method being then sliced on slicer.Compared with conventional paraffin section, frozen section
Have many advantages, such as quick, easy and easy to operate.It is basic in exocuticle, endepidermis cell, the pulp of research jackfruit pulp
When the design feature of the tissues such as cross section, collateral bundle, the amphicribral bundle of tissue, Frozen sections are conducive to carry out cell
The research of biology and molecular biology, and effectively research means are provided, it is convenient for reference, healthy jackfruit group can also be compared
Knit the microstructure difference in the illing tissue with jackfruit.The prior art has the structure to pineapple honey-leaf and flower to be ground
Study carefully, and the rare report of the structural research of fruit, due to the particularity of jackfruit flesh cell structure, frozen section technique is in spinach
It is rarely reported in the research of trailing plants mamoncillo meat.Jackfruit pulp moisture content is more, and ice crystal easily occurs in when general frozen section, causes
Clasmatosis structure is imperfect, or even can not observe the form of full wafer pulp organization.It is cut based on the frost of above-mentioned jackfruit pulp
The problem of piece, proposes a kind of frozen section method, have it is quick, easy, easy to operate, be able to observe that intact cell
Structure and the appearance for reducing ice crystal damage obtain quality and are preferably sliced result.
Summary of the invention
In view of this, solving the above problem the present invention provides a kind of frozen section method of jackfruit pulp.
The technological means that the present invention uses is as follows: a kind of frozen section method of jackfruit pulp, comprising the following steps:
(1) fresh jackfruit pulp is taken to be cut into small pieces, and it is 18~23% that fritter pulp organization, which is placed in containing concentration,
Sucrose FAA fixer in handle 25~35min, then by the pulp organization after fixing process use containing concentration be 18~23%
The phosphate buffer of sucrose embathe 20~30min；
(2) pulp organization after embathing is placed in glycerol, and 40~60min of vacuum suction；
(3) pulp organization after vacuum suction is placed in incubator and is cultivated, recycle the mixing packet of sucrose solution and OTC
It buries agent and carries out 20~40min of embedding；
(4) embedded pulp organization is placed in quick-frozen 35~45s in liquid nitrogen；
(5) quick-frozen good pulp organization is placed in household freezer the 0.8~1.2h that rises again, then is sliced；
(6) dyeing of sarranine aqueous solution is added dropwise in tangential section material, then slice is immersed in 5~10s in distilled water, detergent
2~3 times, 180~220uL Tween-20 is added dropwise in sliced materials surface, covers slide.
Further, in the step (1), the concentration of phosphate buffer is 8~12mmol/L.
Further, in the step (2), the pulp organization after embathing is placed in the glycerol that concentration is 10~20%,
And 40~60min of vacuum suction.
Further, in the step (2), the pulp organization after embathing is placed in the glycerol that concentration is 15%, and true
Empty pump gas 50min.
Further, in the step (3), cultivation temperature is 20~25 DEG C, and incubation time is 1~1.5h.
Further, in the step (3), sucrose solution and OCT embedding medium according to volume ratio be 0.8~1.2:0.8~
1.2 ratio is mixed.
Further, in the step (4), quick freezing temperature is -190~-210 DEG C.
Further, in the step (5), cutting temperature is -18~-21 DEG C, and slice thickness is 7~12 μm.
Further, in the step (6), tangential section material 0.5~1.5% sarranine aqueous solution of dropwise addition dyeing 32~
40min。
Compared with prior art, the beneficial effects of the present invention are:
Firstly, using sucrose-FAA fixer fixing process jackfruit pulp, sucrose alleviates jackfruit as protective agent
The freezing injury of pulp, provides cryoprotection；And physiology of FAA fixer when being to more preferably sample tissue be made to be maintained at sampling
State, being embathed using the phosphate buffer of sucrose is to carry out osmotic protection to fixed sample tissue.
Secondly, being placed in glycerol, and the vacuum suction under certain time, due to the tissue characteristic of jackfruit pulp, pineapple
Compared with the washiness of other plant organ, vacuumizing under certain time is sky in order to make jackfruit pulp for moisture in mamoncillo meat
Chamber is filled by glycerol completely, allows the iuntercellular of jackfruit full of glycerol, to preferably replace the moisture in cell, is vacuumized
Time not enough will lead to the broken of sample tissue.
Again, contain more since the pulp organization of jackfruit compares the plant organs such as other floral organs, stem section and blade
Moisture, be jointly processed by jackfruit Meat Sample using mixing embedding medium after incubator culture, the purpose of incubator be in order to
It makes the glycerol in tissue fully penetrated into sample tissue and stablizes sample tissue state, embedding medium can sufficiently be embedded into sample
Tissue, it is more preferable to the cryoprotection of sample, and embed the time not enough and will lead to the broken of sample tissue.
Then, embedded sample is placed in it is quick-frozen in liquid nitrogen, in order to reduce sample in the residence time of minimum ice crystal band,
Reduce the generation of ice crystal.
Then, quick-frozen good sample is placed in household freezer and is risen again, is sliced, rising again is kept away to soften sample in right amount
Exempt from really up to the mark to cause historrhexis after sample is quick-frozen.
Finally, being dyed, since jackfruit pulp water content is higher, so sample is rushed after quick-frozen dyeing with distilled water
It washes after being easy to cause tissue fragmentation and is flushed away, therefore loose colour is sloughed in unavailable water flushing, can only use distilled water immersion rear decoloring.
The each step of the invention is closely connected, and mutually acts synergistically, carries out to existing Frozen sections operating procedure
Optimization reduces experimental procedure and saves experimental period, reduce the generation of jackfruit flesh cell ice crystal, reduces the broken of cell
It is broken, it ensure that the integrality of eucaryotic cell structure and institutional framework, clearly pulp organization slice map can be obtained.
Detailed description of the invention
Fig. 1 is the quick-frozen jackfruit pulp organization not embedded；
Fig. 2 is the not quick-frozen rear jackfruit pulp organization embedded；
Fig. 3 is the health tissues jackfruit pulp organization of quick-frozen embedding；
Fig. 4 is illing tissue's jackfruit pulp organization of quick-frozen embedding.
Specific embodiment
The principles and features of the present invention are described below, and illustrated embodiment is served only for explaining the present invention, is not intended to
It limits the scope of the invention.
18~23% sucrose solutions: the sucrose solids of 18~23g and the ddH2O mixing of 100mL are taken.
FAA fixer: formalin (40% formaldehyde) 5mL, glacial acetic acid 5mL, 95% alcohol 50mL, distilled water 35mL are mixed
It is even.
Phosphate buffer: PBS, 8~12mmol/L, Ph7.0.
FAA fixer containing 18~23% sucrose: the sucrose solids of 18~23mL and the FAA fixer mixing of 100mL are taken.
Phosphate buffer containing 18~23% sucrose: the phosphate buffer of the sucrose solids and 100mL that take 18~23mL is mixed
It closes.
10~20% glycerol: the ddH2O mixing of 100% glycerol and 100mL of 10~20mL is taken.
The mixing embedding medium of 18~23% sucrose solutions and OCT: by 18~23% sucrose solution and OCT embedding medium according to
The ratio of v/v=0.8~1.2:0.8~1.2 is mixed.
Embodiment 1
A kind of frozen section method of jackfruit pulp, comprising the following steps:
(1) it takes fresh jackfruit pulp to be cut into small pieces, and fritter pulp organization is placed in the sugarcane for being 18% containing concentration
25min is handled in the FAA fixer of sugar, then by the phosphoric acid for the sucrose for being 18% containing concentration of the pulp organization after fixing process
Buffer embathes 20min, and the concentration of phosphate buffer is 8mmol/L.
(2) pulp organization after embathing is placed in concentration is and vacuum suction 15min in 10% glycerol.
(3) pulp organization after vacuum suction is placed in incubator and cultivates 2h, 20 DEG C of cultivation temperature, recycle sucrose molten
The mixing embedding medium of liquid and OTC carry out embedding 20min according to the ratio that volume ratio is 0.8:1.2.
(4) embedded pulp organization is placed in quick-frozen 35s in liquid nitrogen, quick freezing temperature is -190 DEG C.
(5) quick-frozen good pulp organization is placed in household freezer the 0.8~1.2h that rises again, then is sliced, cutting temperature be-
18 DEG C, slice thickness is 7 μm；
(6) tangential section material is added dropwise 0.5% sarranine aqueous solution and dyes 32min, then slice is immersed in 5s in distilled water, washes
It washs material 2 times, 180uL Tween-20 is added dropwise in sliced materials surface, covers slide.
Embodiment 2
A kind of frozen section method of jackfruit pulp, comprising the following steps:
(1) it takes fresh jackfruit pulp to be cut into small pieces, and fritter pulp organization is placed in the sugarcane for being 23% containing concentration
35min is handled in the FAA fixer of sugar, then by the sucrose for being 18~23% containing concentration of the pulp organization after fixing process
Phosphate buffer embathes 30min, and the concentration of phosphate buffer is 12mmol/L.
(2) pulp organization after embathing is placed in concentration is and vacuum suction 25min in 20% glycerol.
(3) pulp organization after vacuum suction is placed in incubator and cultivates 4h, 25 DEG C of cultivation temperature, recycle sucrose molten
The mixing embedding medium of liquid and OTC carry out embedding 40min according to the ratio that volume ratio is 1.2:0.8.
(4) embedded pulp organization is placed in quick-frozen 45s in liquid nitrogen, quick freezing temperature is -210 DEG C.
(5) quick-frozen good pulp organization being placed in household freezer the 1.2h that rises again, then is sliced, cutting temperature is -21 DEG C,
Slice thickness is 12 μm；
(6) tangential section material is added dropwise 1.5% sarranine aqueous solution and dyes 40min, then slice is immersed in 10s in distilled water,
Detergent 3 times, 220uL Tween-20 is added dropwise in sliced materials surface, covers slide.
Embodiment 3
A kind of frozen section method of jackfruit pulp, comprising the following steps:
(1) it takes fresh jackfruit pulp to be cut into small pieces, and fritter pulp organization is placed in the sugarcane for being 20% containing concentration
30min is handled in the FAA fixer of sugar, then by the phosphoric acid for the sucrose for being 20% containing concentration of the pulp organization after fixing process
Buffer embathes 15min, and the concentration of phosphate buffer is 10mmol/L.
(2) pulp organization after embathing is placed in concentration is and vacuum suction 50min in 15% glycerol.
(3) pulp organization after vacuum suction is placed in incubator and cultivates 3h, 22 DEG C of cultivation temperature, recycle sucrose molten
The mixing embedding medium of liquid and OTC carry out embedding 30min according to the ratio that volume ratio is 1:1.
(4) embedded pulp organization is placed in quick-frozen 40s in liquid nitrogen, quick freezing temperature is -200 DEG C.
(5) quick-frozen good pulp organization is placed in household freezer the 1h that rises again, then is sliced, cutting temperature is -20 DEG C, is cut
Piece is with a thickness of 10 μm；
(6) tangential section material is added dropwise 1.0% sarranine aqueous solution and dyes 35min, then slice is immersed in 8s in distilled water, washes
It washs material 3 times, 200uL Tween-20 is added dropwise in sliced materials surface, covers slide.
In order to verify beneficial effects of the present invention, the following verification test of further progress:
Comparative example is set
Comparative example 1
The difference of the comparative example and embodiment 3 is: step (1) uses concentration in the FAA fixer of 15% sucrose
20min is handled, embathes 20min with the phosphate buffer for the sucrose for being 15% containing concentration.
Comparative example 2
The difference of the comparative example and embodiment 3 is: step (1) uses concentration in the PBS fixer of 15% sucrose
30min is handled, embathes 18min with the phosphate buffer for the sucrose for being 15% containing concentration.
Comparative example 3
The difference of the comparative example and embodiment 3 is: step (1) using 4% paraformaldehyde, 2.5% glutaraldehyde,
10mmol/LPBS fixer handles 30min, embathes 20min with the phosphate buffer for the sucrose for being 20% containing concentration.
Comparative example 4
The difference of the comparative example and embodiment 3 is: not carrying out step (2), is not placed in the pulp organization after embathing sweet
In oil, also it is not evacuated.
Comparative example 5
The difference of the comparative example and embodiment 3 is: step (3) does not cultivate pulp organization, directly takes out vacuum
Pulp organization after gas is embedded using the mixing embedding medium of sucrose solution and OTC according to the ratio that volume ratio is 1.5:2.
Comparative example 6
The difference of the comparative example and embodiment 3 is: step (3) does not cultivate pulp organization, directly takes out vacuum
Pulp organization after gas is embedded using OTC embedding medium according to the ratio that volume ratio is 1:1.
Comparative example 7
The difference of the comparative example and embodiment 3 is: step (3) does not cultivate pulp organization, directly takes out vacuum
Pulp organization after gas is embedded using common glue according to the ratio that volume ratio is 1.5:2.
Comparative example 8
The difference of the comparative example and embodiment 3 is: embedded pulp organization is placed in quick-frozen in liquid nitrogen by step (4)
20s。
Comparative example 9
The difference of the comparative example and embodiment 3 is: embedded pulp organization is first placed in quick-frozen 30s in liquid nitrogen, then
It is embedded using common glue according to the ratio that volume ratio is 1.5:2.
Comparative example 10
The difference of the comparative example and embodiment 3 is: embedded pulp organization is placed in quick-frozen in liquid nitrogen by step (4)
48s。
Comparative example 11
The difference of the comparative example and embodiment 3 is: the dyeing of 1.0% sarranine aqueous solution is added dropwise in step (6) tangential section material
30min, then slice is washed with distilled water material 4 times, 50% glycerol is added dropwise in sliced materials surface, covers slide.
One, the method for the present invention is compared with the time cycle of paraffin section production method, the results are shown in Table 1.
Existing paraffin method: it takes fresh jackfruit pulp to be cut into small pieces, and fritter pulp organization is placed in FAA and is fixed
It is handled in liquid, then the pulp organization after fixing process is sufficiently washed, use graded ethanol to be dehydrated step by step as dehydrating agent, dimethylbenzene
It is impregnated as clarifier, embedding after being immersed in tissue using paraffin, then be sliced, open up piece, dyed and etc..
Table 1:
As seen from the above table, the jackfruit pulp Frozen sections that the present invention uses are compared with paraffin method, institute of the present invention
Total time is 5.7h~6.2h, and total time used in paraffin method is 7~8d, and the present invention, which has, reduces experimental procedure
Effect with experimental period is saved, further demonstrates that, Frozen sections are convenient for carrying out jackfruit pulp further structure point
Analysis, by microstructure, is able to observe that flesh cell complete structure and arranging situation.
Two, the integrality for being sliced the method for the method of the embodiment of the present invention 1~3 and comparative example 1~11, cell
Fragmentation degree and dicing effect, the results are shown in Table 2.
Table 2:
As seen from the above table, embodiment 3 is compared with comparative example 1~3, due to the institutional framework feature of jackfruit, fruit of the present invention
Meat handles certain time in the sucrose-FAA fixer of certain concentration, the phosphate buffer of sucrose, has slice integrity degree high, spinach
Trailing plants mamoncillo meat cell integrity is good, and ice crystal amount is few, the excellent advantage of sample tissue microexamination effect.
Embodiment 3 is compared with comparative example 4, due to the tissue characteristic of jackfruit pulp, the frozen section difficulty of Meat Sample
It is greater than other organ-tissues of plant.Moisture is more in jackfruit pulp, therefore compares other plant organ, and glycerol, which vacuumizes, to be set
The moisture needs changed in sample tissue carry out within the regular hour, otherwise will lead to the broken of sample tissue.
Embodiment 3 is compared with comparative example 5~7, since the pulp organization of jackfruit is to plants such as floral organ, stem section and blades
Organ contains more moisture.So the present invention is first cultivated in embedding, in order to make the glycerol in tissue abundant
Sample tissue state is penetrated into sample tissue and stablized, the sucrose that embedding medium is OCT and 20% is reused and mixes by volume
Solvent, compare that pure OCT embedding medium is more preferable to the cryoprotection of sample, and the cryoprotection also than common glue is more preferable.
For embodiment 3 compared with comparative example 8~10, the present invention carries out liquid nitrogen flash freezer after first carrying out mixing embedding medium embedding again,
Cryoprotection is provided for the sample during frozen section, prevents sample tissue broken.
Embodiment 3 is compared with comparative example 11, since jackfruit pulp water content is higher, so sample is used after quick-frozen dyeing
Distilled water flushing is flushed away after being easy to cause tissue fragmentation, therefore loose colour is sloughed in unavailable water flushing, can only use distilled water immersion
Rear decoloring.
It compares non-vacuumize process, embed, quick-frozen (Fig. 1), do not vacuumize, embed, not quick-frozen (Fig. 2), the present invention subtracts
The generation for having lacked jackfruit flesh cell ice crystal reduces the broken of cell, ensure that the integrality of eucaryotic cell structure and institutional framework,
Clearly pulp organization slice map (Fig. 3) can be obtained.Fig. 1, the tissue in 2 are respectively amphicribral bundle and jackfruit
Flesh cell.Tissue in Fig. 3 is the flesh cell of the amphicribral bundle and vascular bundle periphery in healthy jackfruit tissue.
It is the flesh cell of jackfruit and the eucaryotic cell structure of pulp illing tissue in Fig. 4.
To sum up, the frozen section method of a kind of jackfruit pulp of the invention, is fixed including the use of sucrose-FAA fixer
Jackfruit pulp is handled, recycling the phosphate buffer of sucrose to embathe is to carry out osmotic protection to fixed sample tissue.It is placed in
In glycerol, and vacuum suction, it is jointly processed by jackfruit Meat Sample using mixing embedding medium after incubator culture, it will be embedded
Sample be placed in quick-frozen in liquid nitrogen, quick-frozen good sample is placed in household freezer and is risen again, is sliced, after finally carrying out distilled water immersion
Washing.The each step of the invention is closely connected, and mutually acts synergistically, has carried out to existing Frozen sections operating procedure excellent
Change, reduce experimental procedure and saves experimental period.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the present invention.
Claims (9)
1. a kind of frozen section method of jackfruit pulp, which comprises the following steps:
(1) it takes fresh jackfruit pulp to be cut into small pieces, and fritter pulp organization is placed in the sugarcane for being 18~23% containing concentration
Sugar FAA fixer in handle 25~35min, then by the pulp organization after fixing process with containing concentration be 18~23% sugarcane
The phosphate buffer of sugar embathes 20~30min；
(2) pulp organization after embathing is placed in glycerol, and 40~60min of vacuum suction；
(3) pulp organization after vacuum suction is placed in incubator and is cultivated, recycle the mixing embedding medium of sucrose solution and OTC
Carry out 20~40min of embedding；
(4) embedded pulp organization is placed in quick-frozen 35~45s in liquid nitrogen；
(5) quick-frozen good pulp organization is placed in household freezer the 0.8~1.2h that rises again, then is sliced；
(6) dyeing of sarranine aqueous solution is added dropwise in tangential section material, then slice is immersed in 5~10s in distilled water, detergent 2~3
It is secondary, 180~220uL Tween-20 is added dropwise in sliced materials surface, covers slide.
2. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (1)
In, the concentration of phosphate buffer is 8~12mmol/L.
3. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (2)
In, the pulp organization after embathing is placed in the glycerol that concentration is 10~20%, and 40~60min of vacuum suction.
4. a kind of frozen section method of jackfruit pulp according to claim 3, which is characterized in that the step (2)
In, the pulp organization after embathing is placed in the glycerol that concentration is 15%, and vacuum suction 50min.
5. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (3)
In, cultivation temperature is 20~25 DEG C, and incubation time is 1~1.5h.
6. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (3)
In, sucrose solution and OCT embedding medium are mixed according to the ratio that volume ratio is 0.8~1.2:0.8~1.2.
7. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (4)
In, quick freezing temperature is -190~-210 DEG C.
8. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (5)
In, cutting temperature is -18~-21 DEG C, and slice thickness is 7~12 μm.
9. a kind of frozen section method of jackfruit pulp according to claim 1, which is characterized in that the step (6)
In, tangential section material is added dropwise 0.5~1.5% sarranine aqueous solution and dyes 32~40min.
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