CN110434899B - Method and device for preparing umbilical cord segment and application thereof - Google Patents
Method and device for preparing umbilical cord segment and application thereof Download PDFInfo
- Publication number
- CN110434899B CN110434899B CN201910830780.5A CN201910830780A CN110434899B CN 110434899 B CN110434899 B CN 110434899B CN 201910830780 A CN201910830780 A CN 201910830780A CN 110434899 B CN110434899 B CN 110434899B
- Authority
- CN
- China
- Prior art keywords
- container
- umbilical cord
- opening
- preparing
- blade
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 118
- 238000000034 method Methods 0.000 title abstract description 14
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 238000010008 shearing Methods 0.000 claims description 28
- 230000007246 mechanism Effects 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 2
- 239000003292 glue Substances 0.000 abstract description 12
- 230000009471 action Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000013459 approach Methods 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 8
- 238000005520 cutting process Methods 0.000 description 6
- 210000004907 gland Anatomy 0.000 description 5
- 210000003606 umbilical vein Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 210000001644 umbilical artery Anatomy 0.000 description 3
- 210000002808 connective tissue Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26B—HAND-HELD CUTTING TOOLS NOT OTHERWISE PROVIDED FOR
- B26B13/00—Hand shears; Scissors
- B26B13/02—Hand shears; Scissors with moulded-in blades
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26B—HAND-HELD CUTTING TOOLS NOT OTHERWISE PROVIDED FOR
- B26B13/00—Hand shears; Scissors
- B26B13/22—Hand shears; Scissors combined with auxiliary implements, e.g. with cigar cutter, with manicure instrument
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Mechanical Engineering (AREA)
- Organic Chemistry (AREA)
- Forests & Forestry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A method for preparing umbilical cord segment comprises placing umbilical cord in vitro from one end opening of container, passing between blades of cutter body, and stopping at bottom surface of the other end of container; under the action of external force, the blade part of the cutter body moves from the container cavity towards the notch on the side wall of the container and gradually approaches the outer side wall of the container cavity, so as to shear the umbilical cord in the container cavity; simultaneously, under the action of the cutter body, a cover body which is covered on the bottom surface of the other end of the container and is connected with the cutter body is opened to expose the opening at the other end of the container, so that the sheared umbilical cord segment is discharged from the opening at the other end of the container; removing the external force, and returning the blade part of the cutter body to the container cavity; and simultaneously, the cover body connected with the cutter body closes the opening at the other end of the container and covers the bottom surface at the other end of the container. The method provided by the utility model can obtain the umbilical cord segment with uniform length, has a flat section, is easy to identify the position of the Huatong glue, and is beneficial to the standardization of the preparation of the Huatong glue and umbilical mesenchymal stem cells.
Description
Technical Field
The utility model relates to a method for preparing biological materials, in particular to a method and a device for preparing an umbilical cord segment by shearing an umbilical cord, and application of the method and the device in separating and preparing Wharton's jelly in umbilical cord tissues and preparing umbilical cord mesenchymal stem cells.
Background
The umbilical cord is the structure of the linkage between the fetus and the placenta. The shape of the umbilical cord is like a rope, the surface of the umbilical cord is smooth and transparent, connective tissue is contained, and the umbilical cord is a umbilical vein and a pair of umbilical arteries. Cord blood stem cells can be isolated from blood contained in neonatal umbilical cord, and umbilical cord mesenchymal stem cells can be isolated from connective tissue.
In the preparation process of the umbilical cord mesenchymal stem cells, the whole umbilical cord is firstly separated into small sections with the length of about 1 cm-3 cm, and then the Huatong glue is peeled off. The peeling is usually to cut the umbilical cord along the umbilical vein, after the umbilical vein and two umbilical arteries are spread, the surgical knife is used to scrape off the outermost membrane of the umbilical cord, and the rest white jelly-like substance is Huatong glue. And then the Wharton gum is sheared and digested to separate umbilical cord mesenchymal stem cells.
Currently, the umbilical cord cutting into small segments is performed with forceps and scissors in an open space. Because the umbilical cord is rectangular form, and the tissue texture is harder/smooth, and current mode is difficult not only to operate, still skids easily, and it is comparatively laborious to use tweezers to clip the umbilical cord, and the umbilical cord section length that obtains is different and the tangent plane is uneven, is difficult for exposing the gum position of leading to, brings the difficulty for the operation of follow-up peeling off gum of leading to, and implementation efficiency is lower.
The utility model patent 201320295292.7 discloses an umbilical cord clamping and cutting device which has the characteristics of large clamping surface, firm clamping, difficult slipping and convenient shearing operation and comprises a base and a gland, wherein the base and the gland are fixed through a rotating shaft, a square groove is formed in the upper side of the base, an elliptic cylinder is arranged on the lower side of the gland, the long axis of the elliptic cylinder is consistent with the length direction of the gland, the width of the square groove is larger than that of the elliptic cylinder, positioning holes are formed in two ends of the base, and positioning devices are arranged at two ends of the gland.
The utility model patent 20161090405. X discloses an umbilical cord tissue shearing device, wherein the blade part is upwards turned for a certain angle, so that the umbilical cord tissue shearing device is more in line with the mechanical characteristics of a human body, the convenience of use is improved, the number of blades is increased, and the shearing efficiency is greatly improved. The front and back cutter heads are respectively provided with at least two cutting edge parts which are upwards turned by a certain angle.
The prior art proposes several technical solutions for clamping and shearing the umbilical cord, but for the similar way as forceps and scissors, it is usually implemented by two instruments, which are independent from each other and lack cooperation in operation.
Disclosure of Invention
The utility model aims to provide a method for preparing an umbilical cord segment, which ensures that the position of the Huatong gum in an umbilical cord is convenient to identify and is beneficial to the subsequent stripping operation of the Huatong gum.
Another object of the present utility model is to provide a device for preparing an umbilical cord segment, which can complete clamping and shearing of an umbilical cord in one apparatus to obtain an umbilical cord segment with a uniform length, and is beneficial to the subsequent stripping operation of the Huatong glue.
The utility model further aims to provide a device for preparing the umbilical cord segment, which facilitates the identification of the position of the Huatong gum in the umbilical cord and facilitates the subsequent stripping operation of the Huatong gum.
It is a further object of the present utility model to provide a method for preparing an umbilical cord segment, and applications thereof in separation and preparation of gum from China and preparation of mesenchymal stem cells of umbilical cord.
It is a further object of the present utility model to provide an apparatus for preparing an umbilical cord segment, and its use in the isolation and preparation of gum from Huatong and the preparation of mesenchymal stem cells.
The utility model provides a method for preparing an umbilical cord segment, which comprises the following steps:
firstly, placing the isolated umbilical cord from an opening at one end of the container, and stopping at the bottom surface at the other end of the container after passing through the blade space of the cutter body;
then, under the action of external force, the blade part of the cutter body moves from the container cavity towards the notch on the side wall of the container and gradually approaches the outer side wall of the container cavity, and the umbilical cord in the container cavity is sheared; at the same time, the method comprises the steps of,
the acting force of the cutter body is applied to the bottom surface of the other end of the container, and the cover body connected with the cutter body is opened to expose the opening at the other end of the container, so that the sheared umbilical cord segment is discharged from the opening at the other end of the container;
removing the external force, and returning the blade part of the cutter body to the container cavity; at the same time, the method comprises the steps of,
the cover body connected with the cutter body closes the opening at the other end of the container and covers the bottom surface at the other end of the container.
By adopting the method provided by the utility model, the cut umbilical cord segment has a flat section, the position of the Huatong gel is easy to determine, the Huatong gel is stripped from the cut umbilical cord segment, the Huatong gel is obtained, and umbilical cord mesenchymal stem cells are further prepared.
Intermittent acting force is applied to the handle of the cutter body, so that continuous shearing and automatic shearing can be carried out on the umbilical cord, the length of the prepared umbilical cord section is uniform, the section is flat, the position of the Huatong glue is easy to determine, the preparation efficiency of the Huatong glue is obviously improved, the standardized operation standard for preparing umbilical cord mesenchymal stem cells is also facilitated to be established, and the Huatong glue and the standardized preparation of umbilical cord mesenchymal stem cells are realized.
In order to complete the clamping and shearing of the umbilical cord in one instrument, the umbilical cord section with uniform length is obtained, and the subsequent stripping operation of the Huatong glue is facilitated. The utility model also provides a device for preparing the umbilical cord segment, which comprises:
a first container having both ends open for accommodating an umbilical cord;
a second container disposed under the first container and including a first opening communicating with the opening of the first container, an upper end surface of the first opening being not in contact with a lower end surface of the first container, and a shearing space being provided therebetween;
the first cutter body comprises a first blade part, a first connecting part and a first handle part, wherein the first blade part is arranged in the shearing space, the first handle part is arranged outside the side wall of the first container or the side wall of the second container, and the first connecting part is used for driving the first blade part to move in the shearing space;
the second cutter body comprises a second blade part, a second connecting part and a second handle part, the second blade part is arranged in the shearing space, the second handle part is arranged outside the side wall of the first container or the side wall of the second container, and the second connecting part is used for driving the second blade part to move in the shearing space;
the cover body comprises a body and a connecting rod, wherein the body is covered on the second opening, the second opening is closed and opened, one end of the connecting rod is arranged on the body, acting force is applied to the other end of the connecting rod, the body is enabled to displace, the second opening is opened, the acting force is removed, the body is reset, and the second opening is closed.
The other specific embodiment of the device for preparing the umbilical cord section further comprises an elastic mechanism, wherein two ends of the elastic mechanism are respectively propped against the outer side wall of the second container and the connecting rod, the elastic mechanism is elastically deformed under the action of external force, returns after the acting force is removed, and enables the body to reset to close the second opening.
Another embodiment of the device for preparing the umbilical cord segment further comprises a first supporting member which is respectively connected with the connecting rod and the first handle part to realize that the first cutter body is connected with the cover body, and meanwhile, after the acting force is removed, the moving first blade part is reset, the body is reset, and the second opening is closed.
Another specific embodiment of the device for preparing the umbilical cord section further comprises a second supporting member which is respectively connected with the connecting rod and the second handle part to realize that the second cutter body is connected with the cover body, and meanwhile, after the acting force is removed, the moving second blade part is reset, and the body is reset to close the second opening.
In another embodiment of the apparatus for making an umbilical cord section, the first vessel and the second vessel are both cylindrical and coaxial.
Another embodiment of the apparatus for making an umbilical cord segment further comprises a pin inserted into a first connector disposed on an outer edge of the sidewall of the first container and a second connector disposed on an outer edge of the sidewall of the second container and assembled with the first connector and the second connector in a clearance fit, such as: the first and second connection portions rotate (e.g., pivot) about the pin.
In another embodiment of the apparatus for making an umbilical cord section, two ends of the first connecting portion are assembled with the pin and the first bracing member, respectively.
In another embodiment of the apparatus for making an umbilical cord section, two ends of the second connecting portion are respectively assembled with the pin and the first bracing member.
The device for preparing the umbilical cord segment provided by the utility model can obtain the umbilical cord segment with uniform length, has a flat section and is easy to identify the position of the Huatong glue. And stripping the umbilical cord segment to obtain the Huatong glue, and further preparing the umbilical cord mesenchymal stem cells.
The device provided by the utility model obviously improves the preparation efficiency of the Huatong gel, is also beneficial to establishing the standardized operation standard of the preparation of the umbilical cord mesenchymal stem cells, and realizes the standardization of the Huatong gel and the preparation of the umbilical cord mesenchymal stem cells.
The device provided by the utility model has the advantages that the first container enables the long umbilical cord to be easily arranged between the blades, and the first container is matched with the second container to realize the uniformity of the length of the umbilical cord section. The umbilical cord is placed in the container, so that the position of the umbilical cord is relatively fixed in the cut operation, the irregular situation of the cut caused by slipping of the umbilical cord in the handheld operation is reduced, and the cutting operation is more labor-saving.
Under the cooperation of lid for the umbilical cord section of cutting off discharges fast, and the umbilical cord sample easily collects, and length is even, and the tangent plane is level.
Drawings
FIG. 1 is a schematic view of an apparatus for preparing an umbilical cord section according to an embodiment of the present utility model;
FIG. 2 is a schematic view of the apparatus for preparing an umbilical cord section of FIG. 1 according to another embodiment of the present utility model;
FIG. 3 is a schematic view of the apparatus for preparing an umbilical cord section of FIG. 1 according to another embodiment of the present utility model;
FIG. 4 is a schematic view of the apparatus for preparing an umbilical cord section of FIG. 3 at another angle;
FIG. 5 is a schematic view of an embodiment of a vessel of the apparatus for preparing an umbilical cord section of the present utility model shown in FIG. 1;
FIG. 6 is a schematic view of an embodiment of a cap of the apparatus for making an umbilical cord section of FIG. 1 according to the present utility model;
FIG. 7 is a schematic view of an embodiment of a cutter of the apparatus for making an umbilical cord section of the present utility model shown in FIG. 1;
FIG. 8 is a schematic view of an embodiment of a connector of the apparatus for making an umbilical cord segment of the present utility model shown in FIG. 1;
FIG. 9 is a schematic view of an embodiment of the elastic mechanism of the apparatus for preparing an umbilical cord segment of the present utility model shown in FIG. 1.
Detailed Description
The technical scheme of the present utility model is described in detail below with reference to the accompanying drawings. The embodiments of the present utility model are only for illustrating the technical scheme of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical scheme of the present utility model, which is intended to be covered by the scope of the claims of the present utility model.
Fig. 1 is a schematic structural view of an embodiment of the apparatus for manufacturing an umbilical cord according to the present utility model, fig. 2 is a schematic structural view of the apparatus for manufacturing an umbilical cord according to the present utility model shown in fig. 1 at another angle, and fig. 3 is a schematic structural view of the apparatus for manufacturing an umbilical cord according to the present utility model shown in fig. 1 in another state. As shown in fig. 1, 2 and 3, the apparatus for preparing an umbilical cord segment of the present embodiment includes a first container 110, a second container 120, a first cutter body 410, a second cutter body 420 and a cover 300.
Fig. 5 is a schematic structural view of an embodiment of the container of the apparatus for preparing an umbilical cord segment according to the present utility model shown in fig. 1, fig. 2 and fig. 3, and in this embodiment, as shown in fig. 5, the first container 110 is opened at both ends for receiving an umbilical cord, and a first connector 111 is provided at the outer edge thereof. A second container 120 is provided below it, which includes a first opening 121 and a second opening 122, a second connecting member 123 is provided at the outer edge of the sidewall, the first opening 121 communicates with the opening of the first container 110, the end surface of the first opening 121 is not in contact with the lower end surface of the first container 120, and a shearing space 130 is provided therebetween. The cover 300 controls the second opening 122 to be in a closed or open state.
When the first container 110 and the second container 120 are both cylindrical and coaxial, the whole body formed by the first container 110 and the second container 120 is cylindrical or in a truncated cone shape, and the end face of the first opening 121 and the lower end face of the first container 120 are circular. It should be understood that when the outer wall of the lower end of the first container 110 is partially connected to the outer wall of the upper end of the second container 120, the shape of the end face of the first opening 121 and the lower end face of the first container 120 is changed, so that the end face of the first opening 121 and the lower end face of the first container 120 take on non-circular shapes, such as: semicircle surface, sector surface, triangular surface or quadrilateral surface, etc. The pin 200 is inserted into the first connector 111 and the second connector 123 to connect the first container 110 and the second container 120, and fix the shape and volume of the shearing space 130.
Fig. 7 is a schematic structural diagram of a cutter of the apparatus for preparing an umbilical cord section according to an embodiment of the present utility model shown in fig. 1, fig. 2 and fig. 3, and in this embodiment, as shown in fig. 7, the first cutter body 410 includes a first blade 411, a first connecting portion 412 and a first handle 413, the first blade 411 is disposed in the shearing space 130, and the first handle 413 is disposed outside the sidewall of the first container 120. One end of the first connecting portion 412 is assembled with the pin 400 and is in clearance fit, and rotates around the pin 200 to drive the first blade 411 to move (e.g., pivot) in the shearing space 130.
The second cutter body 420 includes a second blade 421, a second connecting portion 422, and a second handle 423, the second blade 421 is disposed in the shearing space 130, and the second handle 423 is disposed outside the sidewall of the second container. One end of the second connecting portion 422 is assembled with the pin 200 and is in clearance fit, and rotates about the pin 400 to move (e.g., pivot) the second blade portion 421 in the shear space 130.
When no force is applied, the first blade 411 and the second blade 421 are located in the shearing space 130, and the umbilical cord enters between the blades, and then reaches the bottom surface of the other end of the second container 120, i.e. the second opening 122 closed by the cover 300.
Fig. 6 is a schematic structural view of an embodiment of a cover of the apparatus for preparing an umbilical cord segment according to the present utility model shown in fig. 1, fig. 2, fig. 3 and fig. 4, and as shown in fig. 6, the cover 300 includes a body 310 and a connecting rod 320, and the body 310 covers the second opening 122 to close and open the second opening 122. One end of the link 320 is disposed on the body 310, and applies a force to the other end thereof, so that the body 310 is displaced, the second opening 122 is opened, the force is removed, the body 310 is reset, and the second opening 122 is closed. To facilitate the closing of the second opening 122 by the cover, in this embodiment, the link 320 is assembled to the lower edge of the second container 120, so that the link 320 pivots.
In this embodiment, the first supporting member 510 is connected to the connecting rod 320 and the first connecting portion 412, so as to connect the cutter to the cover, and after the acting force is removed, the moving first blade 411 is reset, and the body 310 is reset, so as to close the second opening 122. The second supporting member 520 is further connected to the connecting rod 320 and the second connecting portion 422, respectively, to connect the cutter to the cover, and after the force is removed, the moving second blade 421 is reset, and the body 310 is reset, so as to close the second opening 122.
Fig. 8 is a schematic structural view of an embodiment of the support member of the apparatus for preparing an umbilical cord section of the present utility model shown in fig. 1, and as shown in fig. 8, the support member 500 includes a sliding rod 530, and springs 540 are provided at both ends of the sliding rod 530, so that the support member 500 is adapted to the closing and opening operations of the body 310 to the second opening 122 after being connected to the connection parts 412 and 422 of the cutter body and the connecting rod 320.
Fig. 9 is a schematic structural view of an embodiment of an elastic mechanism of the apparatus for preparing an umbilical cord segment according to the present utility model shown in fig. 1, and referring to fig. 9, in this embodiment, an elastic mechanism 600 is further provided, two ends of which are respectively abutted against an outer sidewall of the second container 120 and the connecting rod 320, and are elastically deformed by an external force, and recover after the external force is removed, and the body 310 is reset to close the second opening 122.
The device for preparing the umbilical cord segment provided in this embodiment, in which the umbilical cord is placed from the opening at one end of the first container, passes between the blades of the blade portion and then stops at the bottom surface (i.e., the closed second opening 122) at the other end of the second container.
Then, the blade of the cutter moves from the inside of the container cavity toward the notch formed by the shearing space 130 and the container sidewall and gradually approaches the container cavity outer sidewall under the action of external force, thereby shearing the umbilical cord in the container cavity. At the same time, the second opening 122 is covered by the force of the cutter body, and the cover body connected with the cutter body is opened to expose the opening at the other end of the container, so that the sheared umbilical cord segment is discharged from the second opening 122.
Removing the external force, and returning the blade part to the container cavity; and simultaneously, the cover body connected with the cutter body closes the opening at the other end of the container and covers the bottom surface at the other end of the container.
So reciprocating, can obtain the umbilical cord section of homogeneous length, the tangent plane is level, and the gum position is easily discerned to the Huatong of each umbilical cord section. And stripping from the umbilical cord segment to obtain Huatong glue, such as: cutting umbilical cord along umbilical vein, spreading, scraping umbilical vein and two umbilical arteries with a scalpel, removing the outermost membrane of umbilical cord with a scalpel, removing the rest white jelly-like substance to obtain HUALONG gel, and further preparing umbilical mesenchymal stem cells.
The device provided by the embodiment can be matched with a corresponding method to obviously improve the preparation efficiency of the Huatong gel, is also beneficial to establishing the standardized operation specification of the preparation of the umbilical cord mesenchymal stem cells, and realizes the standardization of the Huatong gel and the preparation of the umbilical cord mesenchymal stem cells.
Claims (10)
1. An apparatus for preparing an umbilical cord section, comprising:
a first container having both ends open for accommodating an umbilical cord;
a second container disposed under the first container and including a first opening and a second opening, the first opening communicating with the opening of the first container, an upper end surface of the first opening being not in contact with a lower end surface of the first container, a shearing space being provided therebetween;
the first cutter body comprises a first blade part, a first connecting part and a first handle part, wherein the first blade part is arranged in the shearing space, the first handle part is arranged outside the side wall of the first container or the side wall of the second container, and the first connecting part is used for driving the first blade part to move in the shearing space;
the second cutter body comprises a second blade part, a second connecting part and a second handle part, the second blade part is arranged in the shearing space, the second handle part is arranged outside the side wall of the first container or the side wall of the second container, and the second connecting part is used for driving the second blade part to move in the shearing space;
the cover body comprises a body and a connecting rod, wherein the body is covered on the second opening, the second opening is closed and opened, one end of the connecting rod is arranged on the body, acting force is applied to the other end of the connecting rod, the body is displaced, the second opening is opened, the acting force is removed, the body is reset, and the second opening is closed;
the pin is inserted into a first connecting piece arranged at the outer edge of the side wall of the first container and a second connecting piece arranged at the outer edge of the side wall of the second container;
the first container and the second container are cylindrical and coaxial.
2. The apparatus for preparing an umbilical cord section as claimed in claim 1, further comprising an elastic mechanism having both ends respectively abutting against the outer sidewall of the second container and the connecting rod, elastically deformed by an external force, restored after the applied force is removed, and restoring the body to close the second opening.
3. The apparatus for preparing an umbilical cord section as claimed in claim 1, further comprising a first stay connected to the connecting rod and the first handle portion, respectively, for enabling the first blade body to be connected to the cover body, and for restoring the first blade portion and restoring the body to close the second opening after the applied force is removed.
4. The apparatus for preparing an umbilical cord section as claimed in claim 1, further comprising a second supporting member connected to the connecting rod and the second handle portion, respectively, for enabling the second blade body to be connected to the cover body, and for restoring the second blade portion and restoring the body to close the second opening after the applied force is removed.
5. The apparatus for preparing an umbilical cord as claimed in claim 1 wherein the pin is assembled with the first connection portion, the first connection portion being in a clearance fit, the first connection portion being rotatable about the pin.
6. The apparatus for preparing an umbilical cord as claimed in claim 1 wherein the pin is assembled with the second connection part, the second connection part being rotated about the pin, in a clearance fit.
7. The apparatus for preparing an umbilical cord section as claimed in claim 1, further comprising a first supporting member, wherein both ends of the first connecting portion are assembled with the pin and the first supporting member, respectively.
8. The apparatus for preparing an umbilical cord section as claimed in claim 1, further comprising a second supporting member, wherein both ends of the second connecting portion are assembled with the pin and the second supporting member, respectively.
9. Use of the device for preparing umbilical cord segments according to any of claims 1-8 for separation and preparation of hua tong gum.
10. Use of a device for preparing umbilical cord segments according to any of claims 1-8 for the preparation of umbilical cord mesenchymal stem cells.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910830780.5A CN110434899B (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
| CN202310512309.8A CN116533291A (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910830780.5A CN110434899B (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310512309.8A Division CN116533291A (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110434899A CN110434899A (en) | 2019-11-12 |
| CN110434899B true CN110434899B (en) | 2023-06-23 |
Family
ID=68439062
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310512309.8A Pending CN116533291A (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
| CN201910830780.5A Active CN110434899B (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310512309.8A Pending CN116533291A (en) | 2019-09-02 | 2019-09-02 | Method and device for preparing umbilical cord segment and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN116533291A (en) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR930003421B1 (en) * | 1991-01-15 | 1993-04-29 | 삼성전자 주식회사 | System self-testing apparatus and method for color video printer |
| JP2006230751A (en) * | 2005-02-25 | 2006-09-07 | San-Ei Faucet Mfg Co Ltd | Pipe cutting implement |
| CN201998188U (en) * | 2010-12-17 | 2011-10-05 | 榆林学院 | Ring-shaped selective protection cutter |
| CN203915003U (en) * | 2014-06-13 | 2014-11-05 | 付玉敏 | A kind of department of obstetrics and gynecology umbilical cord scissors |
| CN207359129U (en) * | 2017-10-24 | 2018-05-15 | 三荣现代农业（成都）有限公司 | Tomato harvesting cut-out equipment |
| CN207724351U (en) * | 2017-12-28 | 2018-08-14 | 赵柯宁 | One kind eating sugarcane device |
| CN207724354U (en) * | 2018-01-19 | 2018-08-14 | 王金星 | Anti-stabbing sharp instrument scissors |
| CN210616596U (en) * | 2019-09-02 | 2020-05-26 | 章毅 | Device for preparing umbilical cord section |
-
2019
- 2019-09-02 CN CN202310512309.8A patent/CN116533291A/en active Pending
- 2019-09-02 CN CN201910830780.5A patent/CN110434899B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110434899A (en) | 2019-11-12 |
| CN116533291A (en) | 2023-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20100050838A1 (en) | Slicing guide device for preparing texture slices, texture slices preparing device, and method for preparing texture slices | |
| US20220022906A1 (en) | Tool for neuroma treatment and nerve regeneration procedures | |
| CN110381848A (en) | Clamp assembly and biopsy sample for operation tool collect component | |
| CA2444981A1 (en) | Apparatus and method for cryoembedding of samples | |
| CN110434899B (en) | Method and device for preparing umbilical cord segment and application thereof | |
| EP3701880A1 (en) | A kit for the extraction of tissues | |
| CN210616596U (en) | Device for preparing umbilical cord section | |
| CN210322346U (en) | Disposable prostate tissue sample negative pressure cutter and external member | |
| CN110074845B (en) | Pressure equalization structure of ultrasonic knife head | |
| CN204914062U (en) | Gluey manual slabbing machine is led to in navel cord china | |
| US20130131544A1 (en) | Biopsy forceps including self aligning jaws with drainage openings | |
| CA3181140A1 (en) | Method and apparatus for dislodging core tissue biopsy samples from core collectors and for storing and preparing samples for pathology | |
| CN101209216A (en) | Cervix coagulating operating knife | |
| CN113288337A (en) | Crescent tong head shearing device for shearing human tissue | |
| CN207300610U (en) | Tumor sample pick device | |
| CN205107883U (en) | Take insulating coating's hot biopsy forceps | |
| CN211534668U (en) | Opening and closing device and opening and closing system | |
| CN108095822A (en) | Vessel forceps | |
| CN112790814B (en) | Quick blocking device of free skin flap transplantation operation blood vessel | |
| CN211665052U (en) | Navel cord huatong's rubber shearing device | |
| CN214048923U (en) | Cavity viscera perforation tissue incising forceps | |
| CN216495513U (en) | Device is got to tumour clamp for medical oncology | |
| CN105034079A (en) | Manual umbilical cord Wharto's jelly dicing machine | |
| CN218979105U (en) | Posterior longitudinal ligament excision device of cervical vertebra | |
| CN107446805A (en) | One kind tissue separation curet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |