CN110433203B - Preservation method of fresh medicine of polygonum bulbiferum - Google Patents
Preservation method of fresh medicine of polygonum bulbiferum Download PDFInfo
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Abstract
The invention provides a preservation method of a fresh medicine of polygonum bulbiferum. The method comprises the following steps: arranging a fresh medicine of the polygonum bulbiferum above a lower fresh-keeping layer, wherein the lower fresh-keeping layer is made of dry sand; an upper fresh-keeping layer is arranged above the fresh medicine of the polygonum bulbiferum, and the upper fresh-keeping layer is made of wood chips. The method firstly proposes that the fresh medicine of the polygonum bulbiferum is stored for a long time by adopting a mode of combining sand and China fir sawdust, the water content of the fresh medicine of the polygonum bulbiferum is high, and the phenomena of mildew, worm damage, oil bleeding, rotting and drying are avoided.
Description
Technical Field
The invention relates to the field of medicines, in particular to a preservation method of a fresh medicine of polygonum bulbiferum.
Background
Polygonum nuciferum L (Polygonum viviparum L.) is a perennial herb of Polygonum of Polygonaceae, and is mainly grown in mountain forests, high mountains or subalpine meadows. The rhizome is used as a medicine, commonly known as plateau bistort rhizome, is bitter and astringent in nature and cool, and has the functions of reducing fever, stopping diarrhea, regulating menstruation, astringing and stopping bleeding. Modern chemical research shows that the polygonum nuciferum contains various components such as volatile oil, phenols (organic acid and flavone), sterol, polysaccharide and the like, wherein the phenols are important active components of the polygonum nuciferum and have antioxidant and bacteriostatic activities.
Fresh medicines, namely fresh traditional Chinese medicinal materials, are generally taken from nature and still in a physiological metabolic state, and keep the freshness and original smell of the medicines by various means, thereby exerting special curative effect. As early as in Shen nong Ben Cao Jing, there are records of using fresh herbs, such as san Xian Tang and Si Sheng Wan. From a large number of ancient herbal and prescription documents, doctors in all generations have a positive understanding of the special effects of fresh herbs on dry herbs, and fresh herbs have special effects on treating difficult and complicated diseases, acute and severe detoxification external treatment and the like, and the concept of 'fresh Chinese herbs and good life' is an empirical summary of a large number of clinical practices for thousands of years. The effective components of the traditional Chinese medicine are reserved, so the traditional Chinese medicine is widely applied in the aspect of traditional Chinese medicine.
Generally, browning is the process by which plants undergo a series of reactions to produce brown polymers. Fresh traditional Chinese medicine can lose water, age, pathogenic microorganism invasion and adversity stress in the storage process to cause quinone substances to accumulate to form a dark brown high polymer, and finally cause browning. The fresh medicine has a self dynamic change process from the beginning of picking, namely self metabolism exists, so that the difficulty of storage and preservation of the fresh medicine is increased, accelerated browning can occur if the fresh medicine is improperly stored, and the browning can cause the content and the structure of active ingredients of the traditional Chinese medicine to be changed so as to influence the efficacy of the traditional Chinese medicine.
The existing common method is that a sand storage method is used for preserving the traditional Chinese medicinal materials, the sand is mainly used for large-volume medicinal materials by utilizing the antibacterial and oxygen-isolating functions of sand, and the polygonum bulborum is small in size and is separately buried in the sand and inconvenient to take. The cedar chips have the advantages of porosity, super-strong water absorption, low material cost, light weight and convenient taking.
Disclosure of Invention
The present application is based on the discovery and recognition by the inventors of the following facts and problems:
the inventor of the application discovers that if the roots and stems of the polygonum bulbiferum are embedded by sand, although the roots and stems of the polygonum bulbiferum do not mildew, the upper surface of the roots and stems of the polygonum bulbiferum is easy to dry, and if the roots and stems of the polygonum bulbiferum are embedded by cedar wood chips, the humidity of the roots and stems of the polygonum bulbiferum is kept good, but the roots and stems of the polygonum bulbiferum at the lower layer are easy to mildew. Based on the discovery, the inventor creatively proposes to combine sand and the Chinese yew wood chips for use, namely, the root and stem of the polygonum bulbiferum is placed on the sand, and the Chinese yew wood chips are laid on the upper surface of the root and stem of the polygonum bulbiferum, so that the problem that the upper surface of the root and stem of the polygonum bulbiferum is dried due to sand embedding can be solved, and the problem that the root and stem of the lower-layer polygonum bulbiferum is easy to mildew due to Chinese yew wood chip embedding can be solved. The practical experiment result also shows that when the fresh red-rooted knotweed herb is stored by combining the sand and the cedar wood chips, the rootstalks of the fresh red-rooted knotweed herb do not mildew, and the rootstalks of the fresh red-rooted knotweed herb are wet and fresh.
Therefore, the invention provides a preservation method of a fresh medicine of the polygonum bulbiferum. According to an embodiment of the invention, the method comprises: arranging a fresh medicine of the polygonum bulbiferum above a lower fresh-keeping layer, wherein the lower fresh-keeping layer is made of dry sand; an upper fresh-keeping layer is arranged above the fresh medicine of the polygonum bulbiferum, and the upper fresh-keeping layer is made of wood chips. The preservation method of the fresh medicine of the polygonum bulbiferum according to the embodiment of the invention firstly proposes the long-term storage of the fresh medicine of the polygonum bulbiferum by adopting the combination mode of the sand and the China fir sawdust, the water content of the fresh medicine of the polygonum bulbiferum is high, and the phenomena of mildew, worm damage, oil bleeding, rotting and drying are avoided.
According to an embodiment of the present invention, the method may further include at least one of the following additional technical features:
according to the embodiment of the invention, the fresh polygonum bulbiferum medicine is sterilized in advance. The inventor finds that the fresh polygonum bulbiferum medicine is sterilized in advance, so that the browning rate of the polygonum bulbiferum can be further remarkably reduced.
According to an embodiment of the present invention, the disinfection treatment is performed in a trichloroisocyanuric acid solution, preferably, the concentration of the trichloroisocyanuric acid solution is 1.0g-2.0 g/L. Trichloroisocyanuric acid (TCCA) is a food-grade disinfectant, and the inventor finds that the trichloroisocyanuric acid solution can kill microorganisms in folds of fresh medicine of polygonum bulbiferum and reduce browning loss caused by mildew of the fresh medicine. It should be noted that the trichloroisocyanuric acid solution according to the embodiment of the present invention is an aqueous solution of trichloroisocyanuric acid, and the purity of trichloroisocyanuric acid used for preparing the trichloroisocyanuric acid solution is 97%, that is, the trichloroisocyanuric acid solution according to the embodiment of the present invention is obtained by dissolving trichloroisocyanuric acid with a purity of 97% in water; the concentration of the trichloroisocyanuric acid solution is 1.0g-2.0g/L, which means that each liter of trichloroisocyanuric acid solution contains 1.0g-2.0g of trichloroisocyanuric acid with the purity of 97%.
According to the embodiment of the invention, the water content of the dry sand is not more than 3%, and the particle size is 0.3-0.5 mm. The dry sand adopted in the method according to the embodiment of the invention is fine and smooth, the interior of the dry sand is saturated by water, but no water exists on the concave-convex parts and the gaps on the surface of the sand, and the sand looks dry.
According to the embodiment of the invention, the thickness of the lower fresh-keeping layer is 1-3 cm. The inventor finds that the thickness of the lower fresh-keeping sand layer is within the range of 1-3cm, and the mildew-proof and dry-proof effects are further improved.
According to the embodiment of the invention, the fresh polygonum bulbiferum is used as a 28-31kg/m medicine2Is arranged above the lower fresh-keeping layer. The inventor finds that the fresh polygonum orientale is 28-31kg/m2The density of the embedded plastic is paved above the lower fresh-keeping layer, so that the fresh-keeping effect can be further improved, and meanwhile, the embedded material can be effectively saved.
According to the embodiment of the invention, the wood chips have the water content of 65-70% and the volume weight of 0.75-0.90g/cm3The pH value is 5.0-5.5. The inventor finds that the water content is 65-70%, and the volume weight is 0.75-0.90g/cm3The wood chips can further improve the moisturizing effect and effectively avoid the problem of dehydration and drying of the fresh medicine on the upper layer; the pH value is 5.0-5.5, which is consistent with the pH value of the soil on the surface of the fresh medicine, thereby effectively avoiding the problem of loss of the fresh medicine components caused by different pH values.
According to the embodiment of the invention, the thickness of the upper fresh-keeping layer is 2-4 cm. The inventor finds that the thickness of the upper fresh-keeping sawdust layer is within the range of 2-4cm, the air permeability of the fresh-keeping layer can be further improved, and the dry-proof effect is further improved.
According to an embodiment of the present invention, the wood chips are cedar chips. The inventor finds that the cedar wood chips contain a large amount of aromatic hydrocarbon substances, are not easy to mildew and breed bacteria, have the pH value of 5.0-5.5 consistent with the pH value of soil on the surface of the fresh medicine, and effectively avoid the problem of loss of effective components of the fresh medicine due to different pH values.
According to the embodiment of the invention, the fresh polygonum bulbiferum medicine provided with the upper and lower freshness keeping layers is placed in an environment at 4-10 ℃. Further reducing the browning rate of the polygonum bulbiferum.
In conclusion, the preservation method of the fresh medicine of the polygonum bulbiferum according to the embodiment of the invention realizes the following technical effects:
1. the used fresh-keeping materials are sand and cedar wood chips, belong to natural materials, are green, environment-friendly and pollution-free, can be in contact with fresh medicines for a long time, and have low cost. The method adopts soaking, sterilizing, and layering, and has effects of sterilizing, keeping moisture, keeping certain oxygen content, and storing for 3 months with high water content without mildew, worm damage, oil bleeding, rot and drying.
2. The sand and cedar chips combined material is adopted, the lower layer of dry sand plays a role in preventing mildew and absorbing redundant water vapor, and the upper layer of cedar chips keep moisture and isolate partial oxygen and carbon dioxide. The dry sand storage environment can inhibit the growth of microorganisms, slow down the rotting and mildew of the polygonum bulbiferum, and the China fir sawdust storage can weaken the respiratory function of the polygonum bulbiferum, reduce the metabolic strength of an enzyme system and reduce transpiration and water loss. 97% trichloroisocyanuric acid (TCCA) is a food-grade disinfectant, can kill microorganisms in folds of the fresh medicine of the polygonum bulbiferum, and reduces browning loss caused by mildew of the fresh medicine.
3. The contents of total sugar, crude protein, starch, fat and amino acid (hydrolysis and dissociation) in the polygonum bulbiferum are slowly reduced in long-term storage, the color browning of the fresh medicine is reduced, the quality of the polygonum bulbiferum in the storage process is obviously improved, and the preservation time of the fresh medicine of the polygonum bulbiferum is prolonged.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative, and is not to be construed as limiting the invention.
According to the specific embodiment of the invention, the method for fresh polygonum bulbiferum medicine provided by the invention can comprise the following steps:
1. soaking fresh Polygonum criopolitanum in 97% trichloroisocyanuric acid solution with concentration of 1.0-2.0 g/L for 10-15min, draining off water, and blowing with natural wind until there is no water drop on the surface of Polygonum criollis;
2. taking a wooden box, spreading a layer of dry river sand (water content less than or equal to 3.0% and particle size 0.3-0.5mm) with thickness of about 1-3cm on the bottom layer, and mixing at a ratio of 28-31kg/m2Uniformly spreading fresh herba Polygoni Capitati, covering with 2-4cm thick lignum Cunninghamiae Lanceolatae chips (water holding amount is 65-70%, volume weight is 0.75-0.90 g/cm)3: pH5.0-5.5), the box cover is covered;
3. placing the wood box containing fresh medicine of herba Polygoni Hydropiperis in a refrigerating chamber, and keeping the storage temperature at 4-10 deg.C.
In the process of exploring the preservation method, the inventor finds that the lower-layer sand is too thick, the space of a wood box is occupied, the weight of the box body is increased, and the sand can absorb more water of the polygonum bulborum, so that the polygonum bulborum at the lower layer is dehydrated and dried; the sand paved on the lower layer is too thin, so that the effect of preventing the bottom layer of the polygonum bulbiferum from mildew can not be achieved. The sand adopted by the application is internally saturated with water, but the sand surface is concave-convex and has no water in gaps, and the sand looks dry. Meanwhile, the inventor also finds that the China fir sawdust is laid too thick, the air permeability is deteriorated, and the cover of the box can be influenced; the cunninghamia lanceolata sawdust is too thin, so that the moisture retention is affected, the fresh medicine on the upper layer is easy to dry, and the problem that all the polygonum bulbiferum cannot be covered is easy to occur.
Embodiments of the present invention will be described in more detail below. The following described embodiments are exemplary and are intended to be illustrative of the invention and are not to be construed as limiting the invention. The trichloroisocyanuric acid used in the following examples was 97% pure, and the trichloroisocyanuric acid with a purity of 97% was purchased from Shanghai Aladdin Biotechnology Ltd. The reagents and apparatus used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1 problems were found
The root and stem of the polygonum bulbiferum newly harvested from Tibetan glossy ganoderma are selected as experimental objects without mildewing and browning. China fir sawdust is purchased from processing factory of Huicheng mineral products in Lingshou county, and has pH of 5.0-5.5.
1.1 methods
(1) Spreading a layer of dry sand (with water content of 2.5% and particle size of 0.3-0.5mm) or China fir chips with water content of 70% on the bottom layer, then weighing about 200 g/box of root and stem of Polygonum dactyloides, putting into a wood box, and finally respectively covering with dry sand (with water content of 2.5% and particle size of 0.3-0.5mm) or China fir chips with water content of 70%; the control group was placed directly in a wooden box;
(2) experimental groups:
treatment group:
a. china fir sawdust: the bottom layer is 75 g (thickness is about 2cm), the rootstock is about 200 g, and the upper layer is covered with 125 g (thickness is about 4 cm);
b. drying sand: a bottom layer of 200 g (thickness about 2cm), a rootstock of about 200 g, and an upper layer of 630 g (thickness about 4 cm);
control group: putting the rootstock into a wooden box: 204 g;
according to the above method, the paving density is about 28kg/m2Each experimental group is made with 3 pieces of parallel wood boxes, and the box covers are covered on the wood boxes of the experimental groups.
(3) Putting into a refrigeration house (the temperature is 4-10 ℃) for storage;
(4) observing on day 30, day 60 and day 90, taking out rhizome, and weighing; the slices were then cut to remove the brown portion, weighed, and the brown ratio calculated as shown below.
1.2 results and analysis:
(1) taking out 1 box of polygonum bulbiferum rhizome which has not been sliced in each experimental group for each time, slicing and weighing, and weighing the weight of the browned polygonum bulbiferum; the browning rate was calculated according to the browning rate formula, and the specific data are shown in table 1 below.
Table 1: influence of different embedding materials on root-stem browning rate of Polygonum criopolitanum
(2) From the data in the table above, the fresh-keeping effect is as follows: the cedar wood chips are better than the sand, and the sand is slightly better than the control;
(3) embedding roots and stems of the polygonum bulbiferum with sand, wherein the roots and stems do not mildew, but the upper surface of the roots and stems of the polygonum bulbiferum is easy to dry, which is probably that dry sand has certain water absorption capacity, and part of water on the surfaces of the roots and stems is released into the sand;
(4) the Chinese fir sawdust embeds roots and stems of the polygonum bulbiferum, the lower-layer roots and stems have a mildewing phenomenon within 30 days, and the humidity of the roots and stems of the polygonum bulbiferum is kept better in the whole aspect, so that the Chinese fir sawdust has better moisture retention and absorption capacity;
(5) the root and stem of Polygonum criopolitanum in the control group were dehydrated and dried, and the root and stem were moldy after 30 days.
1.3 conclusion:
in conclusion, the results and analysis show that the fresh-keeping effect of the cedar wood chips is better than that of the sand, and the sand fresh-keeping effect is better than that of the control group.
Example 2
Selecting the rhizome of the polygonum bulbiferum newly harvested from Tibetan glossy ganoderma as an experimental object.
2.1, method:
(1) spreading a layer of dry sand (with water content of 2.5% and particle size of 0.3-0.5mm) or 65% of cedar chips on the bottom layer, weighing about 500 g/box of root and stem of Polygonum nuciferum, placing into a wood box, and covering with dry sand (with water content of 2.5% and particle size of 0.3-0.5mm) or 65% of cedar chips; the control group was placed directly in a wooden box;
(2) experimental group
Treatment group:
a. china fir sawdust: a layer of cedar wood chips with the thickness of about 2cm is laid on the bottom layer, the rhizome is placed in the bottom layer for about 500 g, and cedar wood chips with the thickness of about 3cm are covered on the upper layer;
b. drying sand: paving a layer of sand with the thickness of about 2cm on the bottom layer, placing about 500 g of rootstocks, and covering sand with the thickness of about 3cm on the upper layer;
c. china fir sawdust and dry sand: a layer of sand with the thickness of about 2cm is paved on the bottom layer, 500 g of rootstocks are placed, and cedar wood chips with the thickness of about 3cm are covered on the upper layer;
control group: placing about 500 grams of roots into a wooden box;
according to the method, the paving density is about 31kg/m2Each experimental group is made with 3 pieces of parallel wood boxes, and the box covers are covered on the wood boxes of the experimental groups.
(3) Putting into a refrigeration house (the temperature is 4-10 ℃) for storage;
(4) observing on day 30, day 60 and day 90, taking out rhizome, and weighing; the slices were then cut to remove the brown portion, weighed, and the brown ratio calculated as shown below.
2.2 results and analysis:
(1) taking out 1 box of polygonum bulbiferum rhizome which has not been sliced in each experimental group for each time, slicing and weighing, and weighing the weight of the browned polygonum bulbiferum; the browning rate was calculated according to the browning rate formula, and the specific data are shown in table 2 below.
Table 2: influence of different embedding materials on root-stem browning rate of Polygonum criopolitanum
(2) From the data in the table above, the fresh-keeping effect is as follows: the ratio of the cedar wood chips and the dry sand is better than that of other treatment groups and control groups;
(3) embedding roots and stems of the polygonum bulbiferum with sand, wherein the roots and stems have no mildew, but the upper surface of the roots and stems of the polygonum bulbiferum is easy to dry;
(4) embedding roots and stems of polygonum capitatum in the cedar wood chips, wherein the lower-layer roots and stems of polygonum capitatum are occasionally mildewed after 30 days, and the humidity of the roots and stems of polygonum capitatum is kept well;
(5) the root and stem of the Polygonum criopolitanum in the control group are easy to dry, and the root and stem of the Polygonum criopolitanum in the control group have mildewed after 30 days;
(6) the rootstocks of the cedar sawdust and dry sand treatment group have no mildew, and the rootstocks are wet and fresh.
2.3 conclusion:
in conclusion, the results and analysis show that the fresh-keeping effect of the cedar wood chips and the dry sand is better than that of other experimental groups.
Example 3
Selecting the rhizome of the polygonum bulbiferum newly harvested from Tibetan glossy ganoderma as an experimental object.
3.1 method:
(1) weighing about 4kg of Polygonum criopolitanum rhizome, placing into 1.5 g/L97% trichloroisocyanuric acid solution (obtained by dissolving 1.5g of trichloroisocyanuric acid with purity of 97% in 1L water), soaking for about 15min, draining, and naturally air drying until there is no water drop on the surface of Polygonum criollis;
(2) spreading a layer of dry sand (with water content of 2.5% and particle size of 0.3-0.5mm) or Chinese fir sawdust with water content of 68% on the bottom of the box, putting the roots and stems of the polygonum bulbiferum soaked with 97% trichloroisocyanuric acid into a wood box, and covering the roots and stems with dry sand (with water content of 2.5% and particle size of 0.3-0.5mm) or Chinese fir sawdust with water content of 68%, wherein the following treatment groups are provided; the roots and stems of polygonum bulbiferum soaked in 97% trichloroisocyanuric acid (TCCA) are directly placed in a wooden box to be used as a control group;
(3)
treatment group:
a. china fir sawdust: spreading a layer of cedar chips with the thickness of about 2cm on the bottom layer, putting about 4kg of rootstocks, and covering the cedar chips with the thickness of about 3cm on the upper layer;
b. drying sand: paving a layer of sand with the thickness of about 2cm on the bottom layer, putting about 4kg of rhizome into the sand, and covering the sand with the thickness of about 3cm on the upper layer;
c. china fir sawdust and dry sand: paving a layer of dry sand with the thickness of about 2cm on the bottom layer, putting 4kg of rhizome and stem, and covering the upper layer with cedar wood chips with the thickness of about 3 cm;
control group: placing about 4kg of rhizomes in a wooden box;
according to the above method, the paving density is about 29kg/m2Each experimental group is made with 3 pieces of parallel wood boxes, and the box covers are covered on the wood boxes of the experimental groups.
(4) Putting into a refrigeration house (the temperature is 4-10 ℃) for storage;
(5) observing on day 30, day 60 and day 90, taking out rhizome, and weighing; the slices were then cut to remove the brown portion, weighed, and the brown ratio calculated as shown below.
3.2 results and analysis:
(1) taking out 1 box of polygonum bulbiferum rhizome which has not been sliced in each experimental group for each time, slicing and weighing, and weighing the weight of the browned polygonum bulbiferum; the browning rate was calculated according to the browning rate formula, and the specific data are shown in Table 3 below.
Table 3: influence of different embedding materials on root-stem browning rate of Polygonum criopolitanum
(2) As seen from the data in the above table, the rate of browning of the roots and stems of Polygonum nuciferum after sterilization with 97% trichloroisocyanuric acid was lower than that of example 2;
(3) in different sampling time periods, the best preservation effect is still as follows: the ratio of the cedar wood dust and the sand is better than that of other treatment groups and control groups;
(4) embedding the roots and stems of the polygonum bulbiferum with sand, wherein the roots and stems do not mildew, but the surfaces of the uppermost layer roots and stems of the polygonum bulbiferum are easy to dry;
(5) embedding roots and stems of polygonum bulborum in the cedar wood chips, wherein the roots and stems of polygonum bulborum at the lower layer have no mildew, and the humidity of the roots and stems of polygonum bulborum is kept well;
(6) the roots and stems of Polygonum criopolitanum in the control group were easily dried, and the respective roots and stems were observed to have mildewed after 90 days.
3.3 conclusion: the rootstocks of the cedar sawdust and sand treatment group have no mildew, and the rootstocks are wet and fresh.
Example 4
In this example, the nutrient content of the roots and stems of polygonum minus in the different treatment groups and the control group in the different preservation time in example 3 was measured.
And (3) measuring moisture: measured according to the baking method in the water content measurement method of "Chinese pharmacopoeia" of 2015 edition.
Determination of crude protein content: weighing a quantitative sample, digesting the quantitative sample by sulfuric acid, decomposing the generated ammonium sulfate by sodium hydroxide to release ammonia, distilling the ammonia into boric acid solution by using water vapor to generate ammonium borate, finally titrating by using strong acid, and calculating the nitrogen content of the test sample according to the consumption of the strong acid; this measurement was performed using an automatic kjeldahl apparatus.
Determination of the total sugar content: measured by a phenol-sulfuric acid method. The regression equation for establishing the glucose standard curve is that y is 10.4597x-0.0474, R21.0000. Weighing the sample, putting the sample into a volumetric flask, ultrasonically dissolving the sample by using hot water at the temperature of 80-90 ℃, and fixing the volume. Diluting the sample, adding 5% phenol solution, shaking, adding concentrated sulfuric acid, shaking, boiling for 4min, taking out, cooling to room temperature, and measuring with ultraviolet-visible spectrophotometer at 485nm wavelength.
And (3) measuring the content of starch: removing soluble sugar with 80% ethanol, hydrolyzing with 6mol/L hydrochloric acid to obtain glucose, calibrating the quality of glucose with alkaline copper tartrate solution, and converting into starch content.
Fat content determination: measuring the fat content in the rhizome of the polygonum bulbiferum by an acid hydrolysis method; and (3) putting a 2mol/L hydrochloric acid water bath sample into a Soxhlet extractor, adding anhydrous ether for extraction, recovering ether, drying the sample, and weighing to obtain the weight of the fat.
Table 4: comparison of nutrient contents of Polygonum Tinctoria rootstocks of different treatment groups in different freshness keeping time in example 3 (unit:%)
As shown in Table 4, the wetting degree of the cedar wood chips and sand group is equivalent to that of the cedar wood chips, but the content of the nutrient components is the highest, namely, the loss of the nutrient components of the cedar wood chips and dry sand combined fresh-keeping is the least.
Example 5
This example measured the free and hydrolyzed amino acid content of Polygonum nuciferum rhizome in the treated and control groups at different freshness times in example 3.
Hydrolysis and determination of the content of free amino acids: preparing hydrolyzed amino acid sample solution (heated and extracted by hydrochloric acid) and free amino acid sample solution (ultrasonically extracted by ultrapure water), performing pre-column derivatization by using phenyl isothiocyanate and triethylamine, establishing an RP-HPLC method, and detecting the contents of the free amino acid and the hydrolyzed amino acid by using a gradient elution method.
Table 5: comparison of the free amino acid content in Polygonum Jumbo rhizome of different treatment groups in different refreshing time in example 3 (unit:%)
As can be seen from Table 5, the total amount of free amino acids in the combination of cedar wood chips and dry sand was the highest at 0.4961% compared to the other groups during the 90-day storage period.
Table 6: comparison of the content of hydrolyzed amino acids in Polygonum Tinctoria rhizome in example 3 at different refreshing time (unit:%)
As shown in Table 6, the content of hydrolyzed amino acids in the combination of cedar wood chips and dry sand in the 30-day, 60-day and 90-day fresh-keeping periods is 4.2372%, 4.0989% and 3.9763%, respectively, which are higher than the content in the corresponding other different treatment and control groups.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (11)
1. A preservation method of a fresh medicine of polygonum bulbiferum is characterized by comprising the following steps:
arranging a fresh medicine of the polygonum bulbiferum above a lower fresh-keeping layer, wherein the lower fresh-keeping layer is made of dry sand;
an upper fresh-keeping layer is arranged above the fresh medicine of the polygonum bulbiferum, and the upper fresh-keeping layer is made of wood chips.
2. The method as claimed in claim 1, wherein the fresh Polygonum criopolitanum medicine is sterilized in advance.
3. The method of claim 2, wherein the disinfection process is performed in a trichloroisocyanuric acid solution.
4. The method of claim 3, wherein the trichloroisocyanuric acid solution has a concentration of 1.0g to 2.0 g/L.
5. The method according to claim 1, wherein the dry sand has a water content of not more than 3% and a particle size of 0.3 to 0.5 mm.
6. The method according to claim 5, wherein the thickness of the lower freshness protection layer is 1-3 cm.
7. The method as claimed in claim 1, wherein the fresh herb of Polygonum criopolitanum is added at a ratio of 28-31kg/m2Is arranged above the lower fresh-keeping layer.
8. The method according to claim 1, wherein the wood chips have a water content of 65 to 70% and a volume weight of 0.75 to 0.90g/cm3The pH value is 5.0-5.5.
9. The method of claim 1, wherein the upper freshness layer has a thickness of 2 to 4 cm.
10. The method of claim 1, wherein the wood chips are cedar chips.
11. The method as claimed in claim 1, further comprising placing the fresh polygonum cuspidatum medicine provided with the upper and lower freshness protection layers in an environment of 4-10 ℃.
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| CN1653916A (en) * | 2005-02-05 | 2005-08-17 | 田景武 | Ginseng fresh-keeping method |
| CN106720275A (en) * | 2016-12-26 | 2017-05-31 | 上海应用技术大学 | A kind of preservation method of bamboo shoots |
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| CN1653916A (en) * | 2005-02-05 | 2005-08-17 | 田景武 | Ginseng fresh-keeping method |
| CN106720275A (en) * | 2016-12-26 | 2017-05-31 | 上海应用技术大学 | A kind of preservation method of bamboo shoots |
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