CN110432914A - Sequencing type is anti-to obscure vacuum blood collection tube - Google Patents
Sequencing type is anti-to obscure vacuum blood collection tube Download PDFInfo
- Publication number
- CN110432914A CN110432914A CN201910681344.6A CN201910681344A CN110432914A CN 110432914 A CN110432914 A CN 110432914A CN 201910681344 A CN201910681344 A CN 201910681344A CN 110432914 A CN110432914 A CN 110432914A
- Authority
- CN
- China
- Prior art keywords
- blood collection
- collection tube
- vacuum blood
- sequence
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150343—Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150763—Details with identification means
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150763—Details with identification means
- A61B5/150786—Optical identification systems, e.g. bar codes, colour codes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
- A61B5/154—Devices using pre-evacuated means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Surgery (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Prevent obscuring vacuum blood collection tube the present invention relates to a kind of sequencing type, it include vacuum blood collection tube body and be set to the intrinsic liquid anticoagulant of vacuum blood collection tube, it is dissolved with specimen discerning sequence in the intrinsic liquid anticoagulant of vacuum blood collection tube, the label of the encoded information of specimen discerning sequence on the books is pasted on the tube wall of the vacuum blood collection tube body.Vacuum blood collection tube provided by the invention is after Samples detection of taking a blood sample, other than the information of objective gene sequence, there are also specimen discerning sequence informations, specimen discerning sequence information can be checked with the sequence label information on request slip and/or receipt, information is consistent both if confirmation, shows that there is no samples to obscure in entire detection process；Whole process is without being transformed existing testing process, not will increase existing workload and changing existing work habit, great practical operability.
Description
Technical field
The present invention relates to a kind of heparin tubes more particularly to a kind of sequencing type to prevent obscuring vacuum blood collection tube.
Background technique
Vacuum blood collection tube is a kind of disposable, achievable negative-pressure vacuum glass tube quantitatively taken a blood sample, including glass tube, lid
Cap and liquid anticoagulant need to match with vein blood taking needle.The principle of vacuum blood collection tube is: will have the glass tube of nut cap pre-
It is first pumped into different vacuum degrees, acquires venous samples using its negative pressure automatic ration, blood taking needle one end is pierced into after human vein separately
The rubber plug of vacuum blood collection tube is inserted into one end, and human vein blood is inside vacuum blood collection tube, under the action of negative pressure, passes through blood sampling
Needle is pumped into blood sample container, and under a venipuncture, multitube acquisition may be implemented without revealing.
Sequencing refers to a kind of method judged the sequence of gene.The different of object can be divided into DNA sequencing again
It is sequenced with RNA；Can be divided into from the development of technology classification: generation Sanger sequencing, in two generations, were sequenced in synthesis and three generations Dan Na
Metre hole sequencing etc..The purposes of sequencing is critically important, and tumour, diagnosis of genetic disorders and noninvasive antenatal detection etc. all include sequencing procedure,
If causing result mistake because sample is obscured, the medical decision making of mistake will lead to, consequence is serious, to personal, family and society
It is all disaster.
The detection of sample includes before detecting, detects to neutralize and detect metasomite, and each link has multiple steps, each step
It is all easy to obscure.Detecting pre-treatment includes blood drawing link, and the transcription for sending the further related to patient information of third party's medical test outside enters,
Although strictly can be avoided the mistake of the overwhelming majority in accordance with blood drawing procedures specification and raising peopleware, as long as someone participates in
All not can guarantee can prevent such mistake；In detection link can be often added in pattern detection some mechanisms for correcting errors or
Operated by two people etc., but this full chain that cannot cover detection, the sample that detection prosomite introduces are obscured and cannot be sent out
It is existing, and such method can only be in fraction laboratory implementation, it is unfriendly to testing laboratory, it is not easy enough, be not suitable with common
It promotes in laboratory.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides that a kind of sequencing type is anti-to obscure vacuum blood collection tube, since source, entirely
Chain covering sample is traced to the source.
The technical solution adopted by the present invention to solve the technical problems is: the sequencing type prevents that obscuring vacuum blood collection tube includes
Vacuum blood collection tube body and be set to the intrinsic liquid anticoagulant of vacuum blood collection tube, it is characterised in that: the vacuum blood collection tube sheet
It is dissolved with specimen discerning sequence in intracorporal liquid anticoagulant, pastes sample on the books on the tube wall of the vacuum blood collection tube body
Identify the label of the encoded information of sequence.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the specimen discerning sequence
It is classified as one section of single-stranded or double-stranded or multi-chain nucleic acid molecule.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the nucleic acid molecules are
Nucleic acid after DNA, one kind of bi-deoxyribose nucleic acid or non-natural nucleic acid or chemical modification.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the non-natural core
Acid includes but is not limited to lock nucleic acid.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, after the chemical modification
Nucleic acid includes but is not limited to 5 end ' of sequence, 3 ends ' and sequence centre modification of nucleic acids.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the specimen discerning sequence
Column exclude the coding with human genome sequence homology.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the specimen discerning sequence
The one or both ends of column have universal primer sequence.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the label uses can
Move adhesive label, at least 2, the label, an indwelling in vacuum blood collection tube body, remaining be pasted on request slip and/or
Receipt.
In a kind of anti-preferred embodiment for obscuring vacuum blood collection tube of sequencing type provided by the invention, the liquid anticoagulant
In also be added with nucleolysis inhibitor.
Compared with prior art, the anti-beneficial effect for obscuring vacuum blood collection tube of sequencing type provided by the invention is: the present invention
The vacuum blood collection tube of offer is after Samples detection of taking a blood sample, and other than the information of objective gene sequence, there are also specimen discerning sequence letters
Breath, specimen discerning sequence information can be checked with the label coding sequence information on request slip and/or receipt, if verification nothing
Accidentally then the two information is consistent, shows that there is no samples to obscure in entire detection process；Whole process is without being transformed existing detection
Process not will increase existing workload and change existing work habit, great practical operability.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing, in which:
Fig. 1 is the anti-structural schematic diagram for obscuring vacuum blood collection tube of sequencing type provided by the invention；
Fig. 2 is the structural schematic diagram for the anti-specimen discerning sequence for obscuring vacuum blood collection tube of sequencing type that Fig. 1 is provided.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that the described embodiments are merely a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
Also referring to Fig. 1 and Fig. 2, the sequencing type prevents that obscuring vacuum blood collection tube 1 includes 11 He of vacuum blood collection tube body
Liquid anticoagulant 12 in vacuum blood collection tube body 11, the vacuum blood collection tube body 11 in the present embodiment are existing
Vacuum blood collection tube, including glass tube 111 and the liquid in nut cap 112 at the top of glass tube, the vacuum blood collection tube body 11
It is dissolved with specimen discerning sequence 13 in anti-coagulants 12, pastes specimen discerning on the books on the tube wall of the vacuum blood collection tube body 11
The label 14 of the encoded information of sequence 13.
Specimen discerning sequence 13 in the present embodiment is generally dissolved in liquid anticoagulant 12 in advance, pours into glass tube 111,
Cover nut cap 112 again, vacuumize, no matter its precedence.
The specimen discerning sequence 13 in the present embodiment is in the nature one section of single-stranded or double-stranded or multi-chain nucleic acid molecule, by
Nucleic acid molecules of several bases to some length between several hundred a bases longs.
The nucleic acid molecules in the present embodiment are DNA, one kind or non-natural of bi-deoxyribose nucleic acid
Nucleic acid after nucleic acid or chemical modification；The non-natural nucleic acid includes but is not limited to lock nucleic acid；Nucleic acid packet after the chemical modification
Include but be not limited to 5 end ' of sequence, 3 ends ' and sequence centre modification of nucleic acids.
Generally, the nucleic acid base of the specimen discerning sequence 13 is generally C, G, A, T (U), 4 letters, it is assumed that sample
The length for identifying sequence is n, and coding category capacity is 4n, exclude the coding with human genome sequence homology, coding rule
It can be with code capacity a variety of, comprehensive various aspects select one kind of optimal reasonable plan, and by the optimal reasonable coding
It is recorded on label 14.
The label 14 in the present embodiment uses removable adhesive label, and removable adhesive label facilitate label turns
It moves, at least 2, the label 14, in vacuum blood collection tube body 11, remaining is pasted on request slip and/or receipt for an indwelling
(not shown), making Samples detection, (including generation Sanger sequencing, two generations are sequenced and three generations's list nano-pore sequencing in synthesis
Deng) the specimen discerning sequence information that obtains afterwards can check with the label coding sequence information on request slip and/or receipt, such as
Then the two information is consistent for fruit confirmation, shows that there is no samples to obscure in entire detection process.
On the basis of the present embodiment, as preferred another embodiment, one end or two of the specimen discerning sequence 13
End has universal primer sequence 132, by taking Fig. 2 in the present embodiment as an example, has universal primer sequence for both ends, including upstream leads to
With primer sequence 132a, downstream universal primer sequence 132b and it is located at upstream universal primer sequence 132a, downstream universal primer sequence
Arrange the nucleic acid coding sequence 13 (i.e. specimen discerning sequence) between 132b.
On the basis of the present embodiment, as preferred another embodiment, in order to avoid specimen discerning sequence is degraded,
Nucleolysis inhibitor (not shown) is also added in the liquid anticoagulant 12.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain similarly includes within scope of patent protection of the invention.
Claims (9)
1. a kind of sequencing type is anti-to obscure vacuum blood collection tube, including vacuum blood collection tube body and it is set to the intrinsic liquid of vacuum blood collection tube
Body anti-coagulants, it is characterised in that: specimen discerning sequence is dissolved in the intrinsic liquid anticoagulant of vacuum blood collection tube, it is described
The label of the encoded information of specimen discerning sequence on the books is pasted on the tube wall of vacuum blood collection tube body.
2. sequencing type according to claim 1 is anti-to obscure vacuum blood collection tube, it is characterised in that: the specimen discerning sequence is
One section of single-stranded or double-stranded or multi-chain nucleic acid molecule.
3. sequencing type according to claim 2 is anti-to obscure vacuum blood collection tube, it is characterised in that: the nucleic acid molecules are deoxidation
Ribonucleic acid, bi-deoxyribose nucleic acid one kind or non-natural nucleic acid or chemical modification after nucleic acid.
4. sequencing type according to claim 3 is anti-to obscure vacuum blood collection tube, it is characterised in that: the non-natural nucleic acid packet
It includes but is not limited to lock nucleic acid.
5. sequencing type according to claim 3 is anti-to obscure vacuum blood collection tube, it is characterised in that: nucleic acid after the chemical modification
Including but not limited to modification of nucleic acids among 5 end ' of sequence, 3 ends ' and sequence.
6. sequencing type according to claim 1 is anti-to obscure vacuum blood collection tube, it is characterised in that: the specimen discerning sequence row
Remove the coding with human genome sequence homology.
7. sequencing type according to claim 1 is anti-to obscure vacuum blood collection tube, it is characterised in that: the specimen discerning sequence
One or both ends have universal primer sequence.
8. any sequencing type is anti-according to claim 1~7 obscures vacuum blood collection tube, it is characterised in that: the label is adopted
With removable adhesive label, at least 2, the label, in vacuum blood collection tube body, remaining is pasted on request slip for an indwelling
And/or receipt.
9. any sequencing type is anti-according to claim 1~7 obscures vacuum blood collection tube, it is characterised in that: the liquid is anti-
Nucleolysis inhibitor is also added in solidifying agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910681344.6A CN110432914A (en) | 2019-07-26 | 2019-07-26 | Sequencing type is anti-to obscure vacuum blood collection tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910681344.6A CN110432914A (en) | 2019-07-26 | 2019-07-26 | Sequencing type is anti-to obscure vacuum blood collection tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110432914A true CN110432914A (en) | 2019-11-12 |
Family
ID=68431632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910681344.6A Pending CN110432914A (en) | 2019-07-26 | 2019-07-26 | Sequencing type is anti-to obscure vacuum blood collection tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110432914A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101770684B1 (en) | 2015-12-30 | 2017-08-23 | 동펑 커머셜 비이클 컴퍼니 리미티드 | Double channel power turbine system and control method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2907544Y (en) * | 2006-05-19 | 2007-06-06 | 山东威高集团医用高分子制品股份有限公司 | Disposable safety blood taking bag |
| CN202277339U (en) * | 2011-08-30 | 2012-06-20 | 付士明 | Vacuum blood collection tube of hypertension blood sample |
| CN102978283A (en) * | 2012-11-20 | 2013-03-20 | 天昊生物医药科技（苏州）有限公司 | Molecular internal standard quality control method and kit for biological sample nucleic acid detection |
| CN204192632U (en) * | 2014-08-27 | 2015-03-11 | 珠海倍健电子科技有限公司 | A kind of vacuum test tube |
| CN204192756U (en) * | 2014-01-23 | 2015-03-11 | 广州正孚检测技术有限公司 | The special sample collection bag of a kind of ethanol illicit drugs inspection |
| CN107201307A (en) * | 2017-05-27 | 2017-09-26 | 广州基赛生物科技有限公司 | A kind of heparin tube and its application |
| CN108244094A (en) * | 2016-12-28 | 2018-07-06 | 江苏为真生物医药技术股份有限公司 | Blood preseration agent and its mating heparin tube |
| CN108893524A (en) * | 2018-06-04 | 2018-11-27 | 北京启衡星生物科技有限公司 | The protective agent of dissociative DNA in blood plasma |
| CN109321523A (en) * | 2018-10-16 | 2019-02-12 | 厦门艾德生物医药科技股份有限公司 | A kind of blood additive |
-
2019
- 2019-07-26 CN CN201910681344.6A patent/CN110432914A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2907544Y (en) * | 2006-05-19 | 2007-06-06 | 山东威高集团医用高分子制品股份有限公司 | Disposable safety blood taking bag |
| CN202277339U (en) * | 2011-08-30 | 2012-06-20 | 付士明 | Vacuum blood collection tube of hypertension blood sample |
| CN102978283A (en) * | 2012-11-20 | 2013-03-20 | 天昊生物医药科技（苏州）有限公司 | Molecular internal standard quality control method and kit for biological sample nucleic acid detection |
| CN204192756U (en) * | 2014-01-23 | 2015-03-11 | 广州正孚检测技术有限公司 | The special sample collection bag of a kind of ethanol illicit drugs inspection |
| CN204192632U (en) * | 2014-08-27 | 2015-03-11 | 珠海倍健电子科技有限公司 | A kind of vacuum test tube |
| CN108244094A (en) * | 2016-12-28 | 2018-07-06 | 江苏为真生物医药技术股份有限公司 | Blood preseration agent and its mating heparin tube |
| CN107201307A (en) * | 2017-05-27 | 2017-09-26 | 广州基赛生物科技有限公司 | A kind of heparin tube and its application |
| CN108893524A (en) * | 2018-06-04 | 2018-11-27 | 北京启衡星生物科技有限公司 | The protective agent of dissociative DNA in blood plasma |
| CN109321523A (en) * | 2018-10-16 | 2019-02-12 | 厦门艾德生物医药科技股份有限公司 | A kind of blood additive |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101770684B1 (en) | 2015-12-30 | 2017-08-23 | 동펑 커머셜 비이클 컴퍼니 리미티드 | Double channel power turbine system and control method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105624335B (en) | A kind of kit of genetic test Middle East respiration syndrome coronavirus | |
| Lippi et al. | Phlebotomy issues and quality improvement in results of laboratory testing | |
| EP3045112B1 (en) | Bodily fluid collection device and testing device | |
| CN103080336A (en) | Kits, devices and methods for detecting chromosome copy number of embryo or tumor | |
| CN106047991A (en) | Methods and kits for detecting kawasaki disease | |
| CN102925562B (en) | Kit and method for detecting aminoglycoside drug-induced deafness-sensitive gene | |
| CN203639460U (en) | Fully enclosed integrated nucleic acid amplification and detection tube | |
| Howanitz et al. | Practices for identifying and rejecting hemolyzed specimens are highly variable in clinical laboratories | |
| CN110432914A (en) | Sequencing type is anti-to obscure vacuum blood collection tube | |
| US20050267384A1 (en) | Blood collection kit adapter | |
| CN106226379A (en) | A kind of biosensor eliminating sample introduction process influence and method of testing | |
| CN104962637A (en) | Kit and primer pair group for detecting drug-induced deafness susceptibility gene mutation site | |
| CN108950066A (en) | Primer and probe and its kit and method based on digital pcr technology detection swine fever virus | |
| CN104328216A (en) | Kit for rapid typing identification detection on Ebola viruses | |
| Dixon et al. | The diagnostic potential of microneedles in infectious diseases | |
| CN109682975B (en) | Hepatitis B detection reagent and application thereof in hepatitis B detection | |
| CN111896759A (en) | Method and device for eliminating influence of sample introduction process and storage medium | |
| CN112126623A (en) | Preparation method of Miltenberger blood type series phenotype red blood cells | |
| CN108977512A (en) | Primer and probe and its kit and method based on digital pcr technology detection haemophilus parasuis | |
| CN110747292A (en) | Method for identifying avian infectious bronchitis QX type virus wild virus and vaccine virus | |
| CN220056885U (en) | Multiplex liquid drop digital PCR (polymerase chain reaction) kit for detecting mucor | |
| NL2033196B1 (en) | Capacitive biosensor for detection of GHB | |
| CN113481293B (en) | Molecular marker for diagnosing diabetic nephropathy and product and application thereof | |
| CN109097497A (en) | A kind of three kit for detecting nucleic acid of human parainfluenza viruses | |
| CN108950065A (en) | Primer and probe and its kit and method based on digital pcr technology detection Porcine epidemic diarrhea virus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191112 |