CN110432423A - A kind of antioxidant of microbial food and preparation method thereof - Google Patents
A kind of antioxidant of microbial food and preparation method thereof Download PDFInfo
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- 235000013305 food Nutrition 0.000 title claims abstract description 37
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 27
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 230000000813 microbial effect Effects 0.000 title claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 46
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000000843 powder Substances 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 26
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 22
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 22
- 239000004310 lactic acid Substances 0.000 claims abstract description 22
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 20
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 19
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 19
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000002609 medium Substances 0.000 claims abstract description 17
- 230000001954 sterilising effect Effects 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 235000009419 Fagopyrum esculentum Nutrition 0.000 claims abstract description 15
- 244000017020 Ipomoea batatas Species 0.000 claims abstract description 15
- 235000002678 Ipomoea batatas Nutrition 0.000 claims abstract description 15
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 15
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 15
- 235000013312 flour Nutrition 0.000 claims abstract description 15
- 239000012530 fluid Substances 0.000 claims abstract description 15
- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- 240000008042 Zea mays Species 0.000 claims abstract description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 14
- 235000005822 corn Nutrition 0.000 claims abstract description 14
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 14
- 239000006228 supernatant Substances 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 239000008223 sterile water Substances 0.000 claims abstract description 7
- 238000005119 centrifugation Methods 0.000 claims abstract description 6
- 239000013049 sediment Substances 0.000 claims abstract description 5
- 240000008620 Fagopyrum esculentum Species 0.000 claims abstract 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 238000010348 incorporation Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 230000003647 oxidation Effects 0.000 abstract description 8
- 238000007254 oxidation reaction Methods 0.000 abstract description 8
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 230000036039 immunity Effects 0.000 abstract description 6
- 231100000252 nontoxic Toxicity 0.000 abstract description 6
- 230000003000 nontoxic effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 235000006708 antioxidants Nutrition 0.000 description 21
- 241000219051 Fagopyrum Species 0.000 description 10
- 238000002835 absorbance Methods 0.000 description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 230000002292 Radical scavenging effect Effects 0.000 description 3
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 3
- 230000019522 cellular metabolic process Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 230000007760 free radical scavenging Effects 0.000 description 3
- 150000003254 radicals Chemical class 0.000 description 3
- 239000012088 reference solution Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 230000002000 scavenging effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Natural products OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 235000019261 food antioxidant Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- -1 hydroxyl radical free radical Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229960004025 sodium salicylate Drugs 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of antioxidant of microbial food and preparation method thereof, comprising: cultivates lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus and saccharomycete on respective culture medium respectively；The lactic acid bacteria of culture, Bifidobacterium, lactobacillus acidophilus and saccharomycete are added in fluid nutrient medium and are shaken up culture, strain stoste is made；Corn flour, dregs of beans, wheat bran, sweet potato powder, buckwheat shell powder and sterile water are mixed, fermentation base-material is made；It sterilizes to fermentation base-material；Closed fermentation cylinder for fermentation is added in strain stoste and fermentation base-material, fermentation liquid is made；Centrifugally operated is carried out to fermentation liquid, centrifugation time is 1~1.5h, discards sediment, collects supernatant；Supernatant is sterilized, sterilizing time is 1~2h, and antioxidant of microbial food is made.Realize at low cost, oxidation resistance is strong, and nontoxic, meets green food safety requirements, can promote human body that the effect of immunity of organisms is absorbed and improved to the conversion of nutriment.
Description
Technical field
The present invention relates to antioxidant of microbial food production technical fields, and it is anti-oxidant to particularly relate to a kind of microbial food
Agent and preparation method thereof.
Background technique
Antioxidant is to prevent the dysgenic substance of oxygen, it be it is a kind of can help to capture and neutralize free radical, thus
The substance that free radical damages human body is dispelled, the antioxidant of human body, which has, itself to be synthesized, and is also had by food supply, people
Class generally can not synthetized oxidation preventive agent, it is necessary to which, from the medium intake of food, the antioxidant in food can protect food from oxygen
Change damage and go bad, the antioxidant in human body alimentary canal has antioxidation, prevents alimentary canal from oxidative damage occurs, and resists
Oxidant can play a role after being absorbed in other histoorgans of body, food-based certain with antioxidation
Extract can be used as treatment drug, and the mechanism of action of antioxidant includes chelating metal ion, removes free radical, single line is quenched
State oxygen, removing oxygen, inhibition oxidase active etc..
In the storage, processing and the process of circulation of food, the grease type component in food is vulnerable to oxygen in air
Oxidation changes colour spoiled so as to cause food, generates harmful substance, lead to food spoilage, thus usually requires in food
Middle addition antioxidant, to guarantee people's safe edible.
And existing food antioxidant leads to higher cost, and food oxydating resistance obtains due to working condition complexity
Less than guarantee.
Summary of the invention
In view of this, oxidation resistance is strong, and nontoxic, meets the purpose of the present invention is to provide a kind of at low cost
Green food safety requirements, the microbial food that can promote human body that immunity of organisms is absorbed and improved to the conversion of nutriment are anti-
Oxidant and preparation method thereof.
Based on a kind of above-mentioned purpose preparation method of antioxidant of microbial food provided by the invention, the preparation method
Include:
S1, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus and saccharomycete are cultivated on respective culture medium respectively,
And respective incubation time is 50~65h, cultivation temperature is 25~35 DEG C；
S2, the lactic acid bacteria cultivated in S1, Bifidobacterium, lactobacillus acidophilus and saccharomycete are added in fluid nutrient medium and are shaken
It is even, 10~15h is cultivated under conditions of being 35~40 DEG C in cultivation temperature, strain stoste is made；
S3, sieving processing carried out to corn flour, dregs of beans, wheat bran, sweet potato powder, buckwheat shell powder respectively, and by corn flour, beans
The dregs of rice, wheat bran, sweet potato powder, buckwheat shell powder and sterile water are mixed, and incorporation time is 2~3h, and mixing temperature is 25~35 DEG C, system
Must ferment base-material；
S4, it sterilizes to fermentation base-material, sterilization time is 1~2h, and sterilising temp is 90~105 DEG C；
S5, closed fermentation cylinder for fermentation is added in strain stoste and fermentation base-material, fermentation time is 15~20 days, is made
Fermentation liquid；
S6, centrifugally operated is carried out to fermentation liquid, centrifugation time is 1~1.5h, discards sediment, collects supernatant；
S7, the supernatant in S6 is sterilized, sterilizing time is 1~2h, and antioxidant of microbial food is made.
Optionally, in step S2, the dosage of lactic acid bacteria is 1~2 part, and the dosage of Bifidobacterium is 2~5 parts by weight, acidophilus
The dosage of lactobacillus is 2~3.5 parts by weight, and the dosage of saccharomycete is 2~4 parts by weight, and the dosage of fluid nutrient medium is 90~100
Parts by weight.
Optionally, in step S2, the dosage of lactic acid bacteria is 2 parts, and the dosage of Bifidobacterium is 5 parts by weight, lactobacillus acidophilus
Dosage be 3.5 parts by weight, the dosage of saccharomycete is 4 parts by weight, and the dosage of fluid nutrient medium is 100 parts by weight.
Optionally, in step S2, the dosage of lactic acid bacteria is 1.5 parts, and the dosage of Bifidobacterium is 4 parts by weight, acidophilus cream bar
The dosage of bacterium is 3 parts by weight, and the dosage of saccharomycete is 3 parts by weight, and the dosage of fluid nutrient medium is 95 parts by weight.
Optionally, in step S3, the dosage of corn flour is 1~2 parts by weight, and the dosage of dregs of beans is 2~4 parts by weight, wheat bran
Dosage be 1~3 parts by weight, the dosage of sweet potato powder is 2~5 parts by weight, and the dosage of buckwheat shell powder is 1~2.5 parts by weight, sterile
The dosage of water is 100~120 parts by weight.
Optionally, in step S3, the dosage of corn flour is 1.5 parts by weight, and the dosage of dregs of beans is 3 parts by weight, the use of wheat bran
Amount is 2 parts by weight, and the dosage of sweet potato powder is 4 parts by weight, and the dosage of buckwheat shell powder is 2 parts by weight, and the dosage of sterile water is 110 weights
Measure part.
Optionally, in step S5, the weight ratio of the dosage of the strain stoste and the fermentation base-material is 40~60:180
~250.
Optionally, in step S5, the weight ratio of the dosage of the strain stoste and the fermentation base-material is 45:210.
Optionally, in step S5, the weight ratio of the dosage of the strain stoste and the fermentation base-material is 60:250.
The present invention also provides a kind of antioxidant of microbial food according to made from preparation method described above.
Through the above technical solutions, the biological metabolism activated product of multiplicity can be obtained in the present invention, a variety of nutriments are rich in,
Have the advantages that at low cost and oxidation resistance is strong and nontoxic, meets green food safety requirements；Repair cell can be played
With the effect for promoting cell metabolism, good ecological environment in human organism is formed, the conversion of nutriment is promoted to absorb and improve
Immunity of organisms is conducive to prevention and rehabilitation to various diseases.
Specific embodiment
For below by the description to embodiment, for example related manufacturing process of a specific embodiment of the invention and operation
Application method etc., is described in further detail, to help those skilled in the art to inventive concept of the invention, technical solution
There is more complete, accurate and deep understanding.
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
The present invention provides a kind of preparation methods of antioxidant of microbial food, wherein the preparation method includes:
S1, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus and saccharomycete are cultivated on respective culture medium respectively,
And respective incubation time is 50~65h, cultivation temperature is 25~35 DEG C；
S2, the lactic acid bacteria cultivated in S1, Bifidobacterium, lactobacillus acidophilus and saccharomycete are added in fluid nutrient medium and are shaken
It is even, 10~15h is cultivated under conditions of being 35~40 DEG C in cultivation temperature, strain stoste is made；
S3, sieving processing carried out to corn flour, dregs of beans, wheat bran, sweet potato powder, buckwheat shell powder respectively, and by corn flour, beans
The dregs of rice, wheat bran, sweet potato powder, buckwheat shell powder and sterile water are mixed, and incorporation time is 2~3h, and mixing temperature is 25~35 DEG C, system
Must ferment base-material；
S4, it sterilizes to fermentation base-material, sterilization time is 1~2h, and sterilising temp is 90~105 DEG C；
S5, closed fermentation cylinder for fermentation is added in strain stoste and fermentation base-material, fermentation time is 15~20 days, is made
Fermentation liquid；
S6, centrifugally operated is carried out to fermentation liquid using centrifuge, centrifugation time is 1~1.5h, discarded sediment, in collection
Clear liquid；
S7, the supernatant in S6 is sterilized, sterilizing time is 1~2h, and antioxidant of microbial food is made.
The biological metabolism activated product of multiplicity can be obtained in the present invention, is rich in a variety of nutriments, has at low cost and antioxygen
The strong advantage of change ability, and it is nontoxic, meet green food safety requirements；Repair cell can be played and promote cell metabolism
Effect forms good ecological environment in human organism, promotes the conversion of nutriment to absorb and improve immunity of organisms, is conducive to
Prevention and rehabilitation to various diseases.
Certainly, sterilization operation here can use skilled artisans appreciate that sterilization mode carry out, for example,
It can be high temperature high pressure sterilizing, or be sterilized etc. using pasteurization, certainly, any appropriate sterilization mode is equal herein
It can be used, the invention is not limited to above-mentioned enumerated modes.Similarly, fluid nutrient medium here can use ability
Field technique personnel it is conventional it will be appreciated that and the Liquid Culture base type that uses, seldom repeat herein.
The dosage of above-mentioned raw materials can select in a wide range, for example, in a kind of preferred embodiment of the invention
In, in order to preferably improve its oxidation resistance, in step S2, the dosage of lactic acid bacteria is 1~2 part, and the dosage of Bifidobacterium is 2
~5 parts by weight, the dosage of lactobacillus acidophilus are 2~3.5 parts by weight, and the dosage of saccharomycete is 2~4 parts by weight, fluid nutrient medium
Dosage be 90~100 parts by weight.
In further preferred embodiment, in step S2, the dosage of lactic acid bacteria is 1 part, and the dosage of Bifidobacterium is 2 weights
Part is measured, the dosage of lactobacillus acidophilus is 2 parts by weight, and the dosage of saccharomycete is 2 parts by weight, and the dosage of fluid nutrient medium is 90 weight
Part.
In one kind more preferably embodiment, the dosage of lactic acid bacteria is 2 parts, and the dosage of Bifidobacterium is 5 parts by weight, thermophilic
The dosage of Lactobacillus lactis is 3.5 parts by weight, and the dosage of saccharomycete is 4 parts by weight, and the dosage of fluid nutrient medium is 100 parts by weight.
Similarly, in a preferred embodiment of the invention, in step S2, the dosage of lactic acid bacteria is 1.5 parts, double
The dosage of discrimination bacillus is 4 parts by weight, and the dosage of lactobacillus acidophilus is 3 parts by weight, and the dosage of saccharomycete is 3 parts by weight, liquid training
The dosage for supporting base is 95 parts by weight.
Similarly, the dosage of each raw material can also select in a wide range in step S3, for example, in one kind of the invention
In preferred embodiment, in step S3, the dosage of corn flour is 1~2 parts by weight, and the dosage of dregs of beans is 2~4 parts by weight, wheat
The dosage of bran is 1~3 parts by weight, and the dosage of sweet potato powder is 2~5 parts by weight, and the dosage of buckwheat shell powder is 1~2.5 parts by weight, nothing
The dosage of bacterium water is 100~120 parts by weight.
In further preferred embodiment, in step S3, the dosage of corn flour is 1.5 parts by weight, and the dosage of dregs of beans is 3
Parts by weight, the dosage of wheat bran are 2 parts by weight, and the dosage of sweet potato powder is 4 parts by weight, and the dosage of buckwheat shell powder is 2 parts by weight, sterile
The dosage of water is 110 parts by weight.
In another preferred embodiment of the invention, in step S5, the strain stoste and the fermentation base-material
The weight ratio of dosage is 40-60:180-250.
In further preferred embodiment, in step S5, the weight of the dosage of the strain stoste and the fermentation base-material
Amount is than being 45:210.
In one kind more preferably embodiment, in step S5, the dosage of the strain stoste and the fermentation base-material
Weight ratio is 60:250.
The present invention also provides a kind of antioxidant of microbial food according to made from preparation method described above.
The present invention will be described in detail by way of examples below.In following embodiment, the fluid nutrient medium is normal
Advise the beef-protein medium used.
Embodiment 1
S1, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus and saccharomycete are cultivated on respective culture medium respectively,
Incubation time is 60h, and cultivation temperature is 30 DEG C；
S2,1 parts by weight lactic acid bacteria, 2 parts by weight Bifidobacteriums, 2 parts by weight lactobacillus acidophilus, 2 parts by weight saccharomycete are added
Enter into 90 part by weight of liquid culture mediums and shake up, incubation time 12h, cultivation temperature is 35 DEG C, and strain stoste is made；
S3, sieving processing is carried out to corn flour, dregs of beans, wheat bran, sweet potato powder, buckwheat shell powder respectively, and 1.5 parts by weight are beautiful
Rice flour, 3 parts by weight dregs of beans, 2 parts by weight wheat bran, 4 parts by weight sweet potato powders, 2 parts by weight buckwheat shell powder and 110 parts by weight sterile waters into
Row mixing, incorporation time 2.5h, mixing temperature are 30 DEG C, and fermentation base-material is made；
S4, it sterilizes to fermentation base-material, sterilization time 1.5h, sterilising temp is 105 DEG C；
S5, closed fermentation cylinder for fermentation is added according to weight ratio for 45:210 in strain stoste and fermentation base-material, fermented
Time is 15 days, and fermentation liquid is made；
S6, centrifugally operated is carried out to fermentation liquid using centrifuge, centrifugation time 1h discards sediment, collects supernatant；
S7, the supernatant in S6 is sterilized, sterilizing time 2h, antioxidant of microbial food A1 is made.
Embodiment 2
It is prepared according to the preparation method of embodiment 1, unlike, in step S2, the dosage of lactic acid bacteria is 2 parts, double
The dosage of discrimination bacillus is 5 parts by weight, and the dosage of lactobacillus acidophilus is 3.5 parts by weight, and the dosage of saccharomycete is 4 parts by weight, liquid
The dosage of culture medium is 100 parts by weight, and antioxidant of microbial food A2 is made.
Embodiment 3
It is prepared according to the preparation method of embodiment 1, unlike, in step S2, the dosage of lactic acid bacteria is 1.5 parts,
The dosage of Bifidobacterium is 4 parts by weight, and the dosage of lactobacillus acidophilus is 3 parts by weight, and the dosage of saccharomycete is 3 parts by weight, liquid
The dosage of culture medium is 95 parts by weight, and the weight ratio in step S5 between strain stoste and fermentation base-material is 60:250, step S6
Middle centrifugation time is 1.5h, and antioxidant of microbial food A3 is made.
Embodiment 4
It is prepared according to the preparation method of embodiment 1, unlike, in step S5 between strain stoste and fermentation base-material
Weight ratio be 60:250, be made antioxidant of microbial food A4.
In the present invention, the biological metabolism activated product of multiplicity can be obtained, be rich in a variety of nutriments, have at low cost and anti-
The strong advantage of oxidability, and it is nontoxic, meet green food safety requirements；Repair cell can be played and promote cell metabolism
Effect, formed human organism in good ecological environment, promote nutriment conversion absorb and improve immunity of organisms, favorably
In prevention and rehabilitation to various diseases.
DPPH Scavenging activity, superoxide radical Scavenging activity and hydroxyl radical free radical are carried out to antioxidant A1~A4 respectively
The measurement of Scavenging activity.
1, DPPH Scavenging activity: the 10 μ L of A1~A4 are separately added into deionized water to 2mL, add 4mL 0.1mmol/
L DPPH- methanol solution adds 450 μ L 50mmol/L Tris-HCL buffer (7.4), water bath with thermostatic control at 25 DEG C
30min is control with Ve, measures absorbance at 517nm using deionized water as reference solution.
DPPH free radical scavenging ability (%)=[(A0-(A1-A2))/A0]×100
In formula, A0For the absorbance of blank control liquid, A1The absorbance of pipe, A are measured for sample2For the suction of sample copy bottom tube
Luminosity.
2, superoxide radical Scavenging activity: using phosphate buffer (0.1M Ph7.4) to 1mL the 10 μ L of A1~A4 respectively,
Then 120 μm of ol/L PMS of 1mL (being prepared with the PBS of 0.1mol/L, pH 7.4) is added, 936 μm of ol/L NADH of 1mL (are used
The PBS of 0.1mol/L, pH 7.4 is prepared) and 300 μm of ol/L NBT of 1mL (being prepared with the PBS of 0.1mol/L, pH 7.4).In ring
At a temperature of border, 5min is reacted.It is control with Ve using deionized water as reference solution.Absorbance is measured at 560nm.
Superoxide radical Scavenging activity (%)=[(A0-(A1-A2))/A0]×100
In formula, A0For the absorbance of blank control liquid, A1The absorbance of pipe, A are measured for sample2For the suction of sample copy bottom tube
Luminosity.
3, hydroxyl radical free radical Scavenging activity: A1~A4 sample is added water into 2mL, is then added to 1.4mL6mmol/LH2O2, so
0.6mL 20mmol/L sodium salicylate and 2mL 1.5mmol/L ferrous sulfate, water bath with thermostatic control 1h at 37 DEG C are added afterwards.With deionization
Water is reference solution, is control with Ve.Absorbance is measured at 562nm.
Hydroxyl radical free radical Scavenging activity (%)=[(A0-(A1-A2))/A0]×100
In formula, A0For the absorbance of blank control liquid, A1The absorbance of pipe, A are measured for sample2For the suction of sample copy bottom tube
Luminosity.The result of test is as shown in the table.
From data above as can be seen that the product oxidation resistance with higher of preparation of the embodiment of the present invention, this is
Due to, can be obtained during preparation multiplicity biological metabolism activated product, be rich in a variety of nutriments, have it is at low cost and
The strong advantage of oxidation resistance, and it is nontoxic, meet green food safety requirements；Repair cell can be played and promote cell generation
The effect thanked forms good ecological environment in human organism, promotes the conversion of nutriment to absorb and improve immunity of organisms, has
Conducive to the prevention and rehabilitation to various diseases.
It should be understood by those ordinary skilled in the art that: the discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under thinking of the invention, above embodiments
Or can also be combined between the technical characteristic in different embodiments, step can be realized with random order, and be existed such as
Many other variations of the upper different aspect of the invention, for simplicity, they are not provided in details.
The embodiment of the present invention be intended to cover fall into all such replacements within the broad range of appended claims,
Modifications and variations.Therefore, all within the spirits and principles of the present invention, any omission, modification, equivalent replacement, the improvement made
Deng should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of antioxidant of microbial food, which is characterized in that the preparation method includes:
S1, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus and saccharomycete are cultivated on respective culture medium respectively, and each
From incubation time be 50~65h, cultivation temperature be 25~35 DEG C；
S2, the lactic acid bacteria cultivated in S1, Bifidobacterium, lactobacillus acidophilus and saccharomycete are added in fluid nutrient medium and are shaken up,
10~15h is cultivated under conditions of being 35~40 DEG C in cultivation temperature, strain stoste is made；
S3, sieving processing carried out to corn flour, dregs of beans, wheat bran, sweet potato powder, buckwheat shell powder respectively, and by corn flour, dregs of beans, wheat
Bran, sweet potato powder, buckwheat shell powder and sterile water are mixed, and incorporation time is 2~3h, and mixing temperature is 25~35 DEG C, and hair is made
Ferment base-material；
S4, it sterilizes to fermentation base-material, sterilization time is 1~2h, and sterilising temp is 90~105 DEG C；
S5, closed fermentation cylinder for fermentation is added in strain stoste and fermentation base-material, fermentation time is 15~20 days, and fermentation is made
Liquid；
S6, centrifugally operated is carried out to fermentation liquid, centrifugation time is 1~1.5h, discards sediment, collects supernatant；
S7, the supernatant in S6 is sterilized, sterilizing time is 1~2h, and antioxidant of microbial food is made.
2. preparation method according to claim 1, which is characterized in that in the step S2, the dosage of lactic acid bacteria is 1~2
Part, the dosage of Bifidobacterium is 2~5 parts by weight, and the dosage of lactobacillus acidophilus is 2~3.5 parts by weight, and the dosage of saccharomycete is 2
~4 parts by weight, the dosage of fluid nutrient medium are 90~100 parts by weight.
3. preparation method according to claim 1 or 2, which is characterized in that in the step S2, the dosage of lactic acid bacteria is 2
Part, the dosage of Bifidobacterium is 5 parts by weight, and the dosage of lactobacillus acidophilus is 3.5 parts by weight, and the dosage of saccharomycete is 4 parts by weight,
The dosage of fluid nutrient medium is 100 parts by weight.
4. preparation method according to claim 1 or 2, which is characterized in that in the step S2, the dosage of lactic acid bacteria is
1.5 parts, the dosage of Bifidobacterium is 4 parts by weight, and the dosage of lactobacillus acidophilus is 3 parts by weight, and the dosage of saccharomycete is 3 weight
Part, the dosage of fluid nutrient medium is 95 parts by weight.
5. preparation method according to claim 1, which is characterized in that in the step S3, the dosage of corn flour is 1~2
Parts by weight, the dosage of dregs of beans are 2~4 parts by weight, and the dosage of wheat bran is 1~3 parts by weight, and the dosage of sweet potato powder is 2~5 weight
Part, the dosage of buckwheat shell powder is 1~2.5 parts by weight, and the dosage of sterile water is 100~120 parts by weight.
6. preparation method according to claim 1 or 5, which is characterized in that in the step S3, the dosage of corn flour is
1.5 parts by weight, the dosage of dregs of beans are 3 parts by weight, and the dosage of wheat bran is 2 parts by weight, and the dosage of sweet potato powder is 4 parts by weight, buckwheat
The dosage of shell powder is 2 parts by weight, and the dosage of sterile water is 110 parts by weight.
7. preparation method according to claim 1, which is characterized in that in the step S5, the strain stoste with it is described
The weight ratio of the dosage of fermentation base-material is 40~60:180~250.
8. preparation method according to claim 1 or claim 7, which is characterized in that in the step S5, the strain stoste and institute
The weight ratio for stating the dosage of fermentation base-material is 45:210.
9. preparation method according to claim 1 or claim 7, which is characterized in that in the step S5, the strain stoste and institute
The weight ratio for stating the dosage of fermentation base-material is 60:250.
10. it is a kind of according to claim 1~any one of 9 described in antioxidant of microbial food made from preparation method.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851259A (en) * | 2011-06-28 | 2013-01-02 | 花王株式会社 | Preparation method of antioxidant |
| CN103555421A (en) * | 2013-11-04 | 2014-02-05 | 上海绿博生物科技发展有限公司 | Microorganism antioxidant of edible vegetable fat as well as preparation method thereof |
| CN104172177A (en) * | 2014-06-11 | 2014-12-03 | 南通昊友食品添加剂有限公司 | Production method of food antioxidant |
| CN104522833A (en) * | 2014-12-16 | 2015-04-22 | 镇江拜因诺生物科技有限公司 | Method for preparing microbial food antioxidant |
| CN105211850A (en) * | 2015-08-26 | 2016-01-06 | 江瀚生物科技（上海）有限公司 | A kind of based on sea-buckthorn and the root of kudzu vine fermentation method and products thereof for antioxidant stoste |
| CN108486005A (en) * | 2018-03-20 | 2018-09-04 | 商丘职业技术学院 | The preparation method and application of antioxidant of microbial food |
| CN109329705A (en) * | 2018-10-31 | 2019-02-15 | 海南职业技术学院 | A kind of production method of antioxidant of microbial food |
| CN109674051A (en) * | 2018-12-07 | 2019-04-26 | 江苏省农业科学院 | A kind of method lactobacillus-fermented enrichment wheat polyphenol and prepare antioxidant |
-
2019
- 2019-07-24 CN CN201910672269.7A patent/CN110432423A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851259A (en) * | 2011-06-28 | 2013-01-02 | 花王株式会社 | Preparation method of antioxidant |
| CN103555421A (en) * | 2013-11-04 | 2014-02-05 | 上海绿博生物科技发展有限公司 | Microorganism antioxidant of edible vegetable fat as well as preparation method thereof |
| CN104172177A (en) * | 2014-06-11 | 2014-12-03 | 南通昊友食品添加剂有限公司 | Production method of food antioxidant |
| CN104522833A (en) * | 2014-12-16 | 2015-04-22 | 镇江拜因诺生物科技有限公司 | Method for preparing microbial food antioxidant |
| CN105211850A (en) * | 2015-08-26 | 2016-01-06 | 江瀚生物科技（上海）有限公司 | A kind of based on sea-buckthorn and the root of kudzu vine fermentation method and products thereof for antioxidant stoste |
| CN108486005A (en) * | 2018-03-20 | 2018-09-04 | 商丘职业技术学院 | The preparation method and application of antioxidant of microbial food |
| CN109329705A (en) * | 2018-10-31 | 2019-02-15 | 海南职业技术学院 | A kind of production method of antioxidant of microbial food |
| CN109674051A (en) * | 2018-12-07 | 2019-04-26 | 江苏省农业科学院 | A kind of method lactobacillus-fermented enrichment wheat polyphenol and prepare antioxidant |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113749212A (en) * | 2021-10-22 | 2021-12-07 | 中南林业科技大学 | Puffed food and preparation method thereof |
| CN113749212B (en) * | 2021-10-22 | 2023-11-28 | 中南林业科技大学 | Puffed food and preparation method thereof |
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