CN110412027B - Dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk - Google Patents
Dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk Download PDFInfo
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- CN110412027B CN110412027B CN201910808823.XA CN201910808823A CN110412027B CN 110412027 B CN110412027 B CN 110412027B CN 201910808823 A CN201910808823 A CN 201910808823A CN 110412027 B CN110412027 B CN 110412027B
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 50
- 210000004251 human milk Anatomy 0.000 title claims abstract description 20
- 235000020256 human milk Nutrition 0.000 title claims abstract description 20
- 238000009792 diffusion process Methods 0.000 claims abstract description 44
- 238000001514 detection method Methods 0.000 claims abstract description 28
- 238000012360 testing method Methods 0.000 claims abstract description 26
- -1 iron ions Chemical class 0.000 claims abstract description 17
- 239000000126 substance Substances 0.000 claims abstract description 8
- 239000002390 adhesive tape Substances 0.000 claims description 33
- 239000000463 material Substances 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 9
- 229920000728 polyester Polymers 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 6
- 239000002861 polymer material Substances 0.000 claims description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 3
- 239000004695 Polyether sulfone Substances 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 229920002492 poly(sulfone) Polymers 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 229920006393 polyether sulfone Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 3
- 239000004800 polyvinyl chloride Substances 0.000 claims description 3
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 11
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract description 11
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 11
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 abstract description 11
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 11
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 11
- 229910052742 iron Inorganic materials 0.000 abstract description 11
- 229910001425 magnesium ion Inorganic materials 0.000 abstract description 11
- 239000011574 phosphorus Substances 0.000 abstract description 11
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 11
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 238000012864 cross contamination Methods 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 235000013336 milk Nutrition 0.000 description 8
- 239000008267 milk Substances 0.000 description 8
- 210000004080 milk Anatomy 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 239000008213 purified water Substances 0.000 description 6
- 230000006835 compression Effects 0.000 description 4
- 238000007906 compression Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 238000004566 IR spectroscopy Methods 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- 239000005725 8-Hydroxyquinoline Substances 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- NGJQIQUNRRHCHB-UHFFFAOYSA-N CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O.OC(=O)C(O)=O Chemical compound CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O.OC(=O)C(O)=O NGJQIQUNRRHCHB-UHFFFAOYSA-N 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KAVGXOKHWPNOAL-UHFFFAOYSA-N benzene-1,3-diol;sodium Chemical compound [Na].OC1=CC=CC(O)=C1 KAVGXOKHWPNOAL-UHFFFAOYSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 description 1
- IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229920003063 hydroxymethyl cellulose Polymers 0.000 description 1
- 229940031574 hydroxymethyl cellulose Drugs 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 229960003540 oxyquinoline Drugs 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 229940005657 pyrophosphoric acid Drugs 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk, which comprises an upper supporting layer and a lower supporting layer which are oppositely arranged, wherein a plurality of reaction layers are composited on the surface of the lower supporting layer at equal intervals, a diffusion layer is composited on the surfaces of the reaction layers, and a hydrophilic layer is composited between the diffusion layer and the upper supporting layer. According to the invention, the plurality of reaction layers are arranged, different detection items in the reaction layers are loaded with different reaction reagents, and detection of different substances on the reagent strip can be realized after one-time dropwise adding of the sample, so that the reagent strip can be simultaneously used for detecting protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in breast milk, and as the reaction layers are not connected, cross contamination among detection substances does not occur, so that the test result is accurate, and the test is convenient.
Description
Technical Field
The invention belongs to the field of measurement of parameters in breast milk, and relates to a dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk.
Background
The breast milk is a natural nutrition for babies, and the components of the breast milk directly influence the growth, intelligence development and digestion capacity of the babies, so the breast milk cannot be replaced by the formula milk. The concentration of protein, calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus in the milk has important significance for the nutrition value of the milk and the intake of babies, and through milk nutrition ingredient detection, a user can be reminded of adjusting the food structure of the user and supplementing nutrition ingredients, so that the health of the milk is promoted, the milk quantity is increased, the milk ingredients are improved, substances harmful to the babies are prevented from being produced in the milk, and the milk is better adapted to the normal development needs of the babies.
Protein, calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus detection methods are numerous. The method for measuring the liquid is usually ultrasonic infrared spectrometry, liquid biochemical reagent method and dry chemical reagent method.
Compared with the ultrasonic infrared spectrometry and the liquid biochemical reagent method, the dry chemical reagent strip has the characteristics of low cost, small required sample size, short detection time, simple operation, convenience, flexibility, small pollution and the like.
At present, dry chemical reagent strips for detecting proteins, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus are mostly foreign products (such as Qiansheng, aikolai, fuji and Roche), and the dry chemical reagent strips have the problems of complex production process, higher requirements on materials and equipment, easiness in generating a steam drum, excessive liquid at a sample injection end, single detection item and the like. In addition, chinese patent CN201410532472.1 discloses a method for preparing a multi-parameter rapid detection card for dairy products, which needs to pretreat the dairy product sample during the detection, immerse the detection card into the sample after the pretreatment, and take out the sample to be compared with the colorimetric card, thus the problems of serious cross contamination, large required sample size, complex operation and inaccurate detection result can exist. Chinese patent CN98109147.4 discloses a reagent strip for visual measurement of breast milk and calcium, chinese patent CN20120130424.0 discloses a dry test piece for calcium measurement and chinese patent CN298115107.8 discloses a dry analysis unit for protein detection, and the methods in these patents are all to detect one item qualitatively or quantitatively alone, and cannot detect multiple items simultaneously. At present, no dry chemical reagent strip capable of simultaneously detecting protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in breast milk exists.
Disclosure of Invention
The invention aims to provide a dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk, a plurality of reaction layers are arranged, and different reagents loaded by different detection items in the reaction layers can be detected on the reagent strip after a sample is dripped once, so that the dry chemical reagent strip can be simultaneously used for detecting protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in the breast milk, and because the reaction layers are not connected, cross contamination among detection substances does not occur, thus not only ensuring accurate test results, but also facilitating the test
The aim of the invention can be achieved by the following technical scheme:
The utility model provides a dry chemical reagent strip of simultaneous quantitative determination breast milk multiparameter, includes relative last supporting layer and the lower supporting layer that sets up, and the surface equidistance of lower supporting layer is compound has a plurality of reaction layers, has the diffusion layer at the surface complex of a plurality of reaction layers simultaneously to complex has the hydrophilic layer between diffusion layer and last supporting layer.
Further, the surface bonding of lower supporting layer has the double faced adhesive tape, and the length and the width of lower supporting layer are the same with the double faced adhesive tape, and open on the surface of lower supporting layer has a plurality of test holes that the equidistance was arranged and is not linked together to every test hole is circular and the internal diameter is the same, and open on the surface of double faced adhesive tape has a plurality of circular through holes corresponding with the test hole.
Further, a plurality of reaction layers are in one-to-one correspondence with a plurality of permeation holes on the surface of the double faced adhesive tape, the center of each reaction layer is coincided with the center of the permeation hole, and the reaction layers are adhered to the permeation holes of the double faced adhesive tape.
Further, the width of the diffusion layer is 3-4mm larger than that of the reaction layer, the bottom surface of the diffusion layer is in compression connection with the surface of the reaction layer, and two sides of the diffusion layer extend out of two sides of the surface of the reaction layer and are then adhered to the surface of the double faced adhesive tape.
Further, the width of the hydrophilic layer is 3-4mm larger than that of the diffusion layer, meanwhile, the width of the hydrophilic layer is 4-5mm smaller than that of the double faced adhesive tape, the bottom surface of the hydrophilic layer is in compression connection with the surface of the diffusion layer, and two sides of the hydrophilic layer extend out of two sides of the surface of the diffusion layer and are then adhered to the surface of the double faced adhesive tape.
Further, the widths of the upper supporting layer and the lower supporting layer are the same, and meanwhile, the middle part of the surface of the upper supporting layer is provided with a sample injection hole, and two sides of the upper supporting layer extend out of the surface of the hydrophilic layer and are then adhered to the surface of the double faced adhesive tape.
Further, the upper supporting layer and the lower supporting layer are both made of high-molecular polymer materials, wherein the high-molecular polymer materials are one of polycarbonate, polystyrene, polyvinyl chloride or polyethylene.
Further, the hydrophilic layer is made of a polyester material, and one of polyvinylpyrrolidone, polyethylene glycol or polyvinyl alcohol is impregnated on the surface of the polyester material.
Further, the diffusion layer is made of one or more of porous nylon, polypropylene, polyester fiber, cotton fabric or mesh cloth.
Further, the reaction layer is a polysulfone membrane or a polyether sulfone membrane.
The invention has the beneficial effects that:
1. According to the invention, the plurality of reaction layers are arranged, and different test reagents loaded in the reaction layers are realized, so that the detection of different substances on the reagent strip can be realized after one-time dripping of the sample, and further the reagent strip can be simultaneously used for detecting the protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in breast milk, and the reaction layers are hesitated to be not connected, so that cross contamination among all the detection substances can not occur, the test result is accurate, and the test is convenient.
2. The invention adopts one dry chemical reagent strip to simultaneously realize the detection of six detection items of calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in the sample, thereby ensuring more accurate detection results, convenient operation, reducing test samples, reducing environmental pollution, shortening detection time, reducing cost and being suitable for large-scale popularization in families.
3. According to the invention, the dry chemical reagent strip is prepared from the membrane materials such as the supporting layer, the diffusion layer, the filter layer, the reaction layer and the double faced adhesive tape, and a siphon system is formed by superposition, so that the detection speed and the accuracy of a result are further ensured, and a plurality of detection parameters can be obtained simultaneously.
Drawings
The present invention is further described below with reference to the accompanying drawings for the convenience of understanding by those skilled in the art.
FIG. 1 is an exploded view of the structure of a dry chemical reagent strip according to the present invention.
Detailed Description
Example 1:
The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk comprises an upper supporting layer 1 and a lower supporting layer 2 which are oppositely arranged, wherein a plurality of reaction layers 5 are composited on the surface of the lower supporting layer 2 at equal intervals, a diffusion layer 4 is composited on the surface of the plurality of reaction layers 5, and a hydrophilic layer 3 is composited between the diffusion layer 4 and the upper supporting layer 1;
The surface of the lower supporting layer 2 is adhered with double faced adhesive tape 6, the length and width of the lower supporting layer 2 are the same as those of the double faced adhesive tape 6, the surface of the lower supporting layer 2 is provided with a plurality of test holes 21 which are arranged at equal intervals and are not communicated, each test hole 21 is round and has the same inner diameter, the inner diameter is 4mm, and the surface of the double faced adhesive tape 6 is provided with a plurality of round permeation holes 61 corresponding to the test holes 21; the change of the reaction color and the reflectance on each reaction layer 5 can be easily observed and measured through the test holes 21 and the circular transmission holes 61;
The reaction layers 5 are in one-to-one correspondence with the transmission holes 61 on the surface of the double faced adhesive tape 6, the center of each reaction layer 5 is coincident with the center of the transmission hole 61, and the reaction layers 5 are directly adhered to the transmission holes 61 of the double faced adhesive tape 6;
The width of the diffusion layer 4 is 3-4mm larger than the width of the reaction layer 5, the bottom surface of the diffusion layer 4 is in compression connection with the surface of the reaction layer 5, meanwhile, as the width of the diffusion layer 4 is larger than the reaction layer 5, two sides of the diffusion layer 4 extend out of two sides of the surface of the reaction layer 5 and then are adhered to the surface of the double faced adhesive tape 6, and the diffusion layer 4 is fixed through the double faced adhesive tape 6;
The width of the hydrophilic layer 3 is 3-4mm larger than the width of the diffusion layer 4, meanwhile, the width of the hydrophilic layer 3 is 4-5mm smaller than the width of the double faced adhesive tape 6, the bottom surface of the hydrophilic layer 3 is in compression connection with the surface of the diffusion layer 4, meanwhile, as the width of the hydrophilic layer 3 is larger than the width of the diffusion layer 4 and the width of the hydrophilic layer 3 is smaller than the width of the double faced adhesive tape 6, two sides of the hydrophilic layer 3 extend out of two sides of the surface of the diffusion layer 4 and then are adhered to the surface of the double faced adhesive tape 6, and the hydrophilic layer 3 is fixed through the double faced adhesive tape 6;
The widths of the upper supporting layer 1 and the lower supporting layer 2 are the same, the thicknesses of the upper supporting layer 1 and the lower supporting layer 2 are 0.4mm, the sizes of the upper supporting layer 1 and the lower supporting layer 2 are 48 multiplied by 6mm, meanwhile, a sample injection hole 11 is formed in the middle of the surface of the upper supporting layer 1, the sample injection hole 11 is rectangular, the sizes of the sample injection holes are 4 multiplied by 5mm, and the sample injection holes 11 are used for dropwise adding samples; the width of the upper supporting layer 1 is larger than that of the hydrophilic layer 3 and is equal to that of the double faced adhesive tape 6, so that two sides of the upper supporting layer 1 extend out of the surface of the hydrophilic layer 3 and then are adhered to the surface of the double faced adhesive tape 6, and the upper supporting layer 1 is fixed through the double faced adhesive tape 6; therefore, the reaction layer 5, the diffusion layer 4, the hydrophilic layer 3, the upper support layer 1 and the lower support layer 2 are all fixed through one layer of double faced adhesive tape 6 by different widths, so that not only is the fixed material saved, but also the blocking among the reaction layer 5, the diffusion layer 4 and the hydrophilic layer 3 reduced, the rapid permeation of detection liquid is facilitated, and the upper support layer 1 and the lower support layer 2 can be recycled by directly tearing the side from the double faced adhesive tape 6 after the use is finished, and the use is convenient and the recycling is convenient;
The lengths of the upper supporting layer 1, the lower supporting layer 2, the hydrophilic layer 3 and the diffusion layer 4 are the same, so that the sides of the prepared reagent strips are tidy, and the influence on the permeability caused by insufficient contact of the detection liquid with the next layer when the detection liquid with the shorter length is arranged on the side of the previous layer is prevented;
the specific use method of the dry chemical reagent strip is as follows:
Firstly, dripping a sample on a reagent strip through a sample inlet 11;
and secondly, the sample passes through the sample inlet 11 and then directly reaches the surface of the hydrophilic layer 3, uniformly and rapidly diffuses leftwards and rightwards through the hydrophilic layer 3, and downwards diffuses to the diffusion layer 4, then reaches the reaction layer 5 to react with the reagent, and finally the test holes of all the detection items show different color changes according to the concentration of the substances to be detected in the sample so as to realize quantitative detection.
Example 2:
The upper supporting layer 1 and the lower supporting layer 2 are made of high molecular polymer materials, wherein the high molecular polymer materials are one of polycarbonate, polystyrene, polyvinyl chloride or polyethylene, and the upper supporting layer 1 and the lower supporting layer 2 play a main supporting role;
The hydrophilic layer 3 is made of a polyester material, one of polyvinylpyrrolidone, polyethylene glycol or polyvinyl alcohol is impregnated on the surface of the polyester material, and a plurality of diffusion holes are uniformly distributed on the surface of the hydrophilic layer 3, wherein the aperture size of the diffusion holes is 10-500um, and the thickness of the diffusion holes is 1-20um, and the main purpose of the hydrophilic layer is to enable a sample to diffuse rapidly;
The diffusion layer4 is made of one or more of porous nylon, polypropylene, polyester fiber, cotton fabric or mesh cloth, the pore size is 20-200um, the thickness is 0.1-0.4mm, in order to enable a sample to be uniformly and rapidly diffused to the reaction layer, the material of the diffusion layer4 is subjected to hydrophilic treatment after being impregnated with a surfactant, wherein the surfactant is a nonionic surfactant such as polyethylene glycol octyl phenyl ether or polysorbate, and the diffusion layer can be treated in an impregnation mode;
The reaction layer 5 is a polysulfone membrane or a polyether sulfone membrane, wherein the pore size of the membrane is 0.4-7um, and the thickness of the membrane is 80-20um; meanwhile, before the reagent strip is prepared, different test reagents are immersed in the reaction layer 5, and after the test reagents are dried, the reaction layer 5 is bonded, wherein the test reagents correspond to different test reagents according to proteins, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus to be detected in a sample, so that six different test reagents capable of respectively detecting proteins, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus are loaded in the prepared 6 reaction layers 5.
Example 3:
The reagent for detecting protein comprises 15-30g of oxalic acid, 0.5-2g of potassium oxalate, 0.5-2g of ammonium molybdate, jiao Fengong 0.01.01-1 g of triton, 0.01-0.1g of tween, 0.01-0.5g of hydroxymethyl cellulose sodium, and 50-100ml of purified water or distilled water.
The reagent for detecting calcium ions comprises 10-20g of pyrophosphoric acid, 0.04-0.1g of azo arsine-III, 3-10ml of ethanol, 0.2-1g of trehalose, 0.1-0.5g of 8-hydroxyquinoline, 0.05-0.2g of triton, 0.2-1g of cyclodextrin, 0.1-1ml of hydrochloric acid and 100-200ml of purified water or distilled water.
The reagent for detecting magnesium ions comprises 5-10g of boric acid, 0.1-2g of dimethylaniline blue, 1-5g of sucrose, 1-5ml of methanol, 0.1-0.5g of oxalic acid tetraacetic acid, 0.05-0.2g of triton, 0.2-1g of polyethylene glycol, 0.01-0.5g of sodium hydroxide and 60-120ml of purified water or distilled water.
The reagent for detecting zinc ions comprises 10-30g of 2- (N-morpholino) ethanesulfonic acid, 0.1-2g of 4- (2-pyridine azo) resorcinol sodium salt, 5-10ml of dimethyl sulfoxide, 0.5-2g of sucrose, 0.2-0.6g of thiourea, 0.1-1g of sodium citrate, 0.5-5g of acrylamide, 0.01-1g of sodium hydroxide and 100-200ml of purified water or distilled water.
The reagent for detecting iron ions comprises 5-10g of glycine, 0.05-1g of ferrozine, 3-10ml of ethanol, 0.05-0.4g of ascorbic acid, 0.01-0.1g of potassium ferrocyanide, 0.1-1g of sodium citrate, 0.1-2g of ethylene glycol, 0.5-10ml of hydrochloric acid and 100-200ml of purified water or distilled water.
The reagent for detecting inorganic phosphorus comprises 15-40g of citric acid, 0.01-0.5g of malachite green, 1-5ml of ethanol, 0.5-2g of trehalose, 0.01-0.3g of sodium citrate, 0.05-1g of bovine serum albumin, 0.3-2g of urea, 1-8ml of hydrochloric acid and 50-120ml of purified water or distilled water.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (4)
1. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk is characterized by comprising an upper supporting layer (1) and a lower supporting layer (2) which are oppositely arranged, wherein a plurality of reaction layers (5) are composited on the surface of the lower supporting layer (2) at equal intervals, a diffusion layer (4) is composited on the surface of the plurality of reaction layers (5), and a hydrophilic layer (3) is composited between the diffusion layer (4) and the upper supporting layer (1);
The surface of the lower supporting layer (2) is adhered with a double-sided tape (6), the length and the width of the lower supporting layer (2) are the same as those of the double-sided tape (6), a plurality of test holes (21) which are equidistantly arranged and are not communicated are formed in the surface of the lower supporting layer (2), each test hole (21) is round and has the same inner diameter, and a plurality of round penetrating holes (61) corresponding to the test holes (21) are formed in the surface of the double-sided tape (6);
The reaction layers (5) are in one-to-one correspondence with the transmission holes (61) on the surface of the double faced adhesive tape (6), the center of each reaction layer (5) is coincident with the center of the transmission hole (61), and the reaction layers (5) are adhered to the transmission holes (61) of the double faced adhesive tape (6);
the upper supporting layer (1) and the lower supporting layer (2) are made of high-molecular polymer materials, wherein the high-molecular polymer materials are one of polycarbonate, polystyrene, polyvinyl chloride or polyethylene;
The hydrophilic layer (3) is made of polyester material, and one of polyvinylpyrrolidone, polyethylene glycol or polyvinyl alcohol is impregnated on the surface of the polyester material;
The diffusion layer (4) is made of one or more of porous nylon, polypropylene, polyester fiber, cotton fabric or mesh cloth;
the reaction layer (5) is a polysulfone membrane or a polyether sulfone membrane;
the specific use method of the dry chemical reagent strip is as follows:
Firstly, dripping a sample on a reagent strip through a sample injection hole (11);
Secondly, the sample passes through the sample injection hole (11) and then directly reaches the surface of the hydrophilic layer (3), uniformly and rapidly diffuses leftwards and rightwards through the hydrophilic layer (3) and downwards diffuses to the diffusion layer (4), then reaches the reaction layer (5) to react with the reagent, and finally the quantitative detection is realized by showing different color changes of the test holes of each detection item according to different concentrations of substances to be detected in the sample;
The reaction layer (5), the diffusion layer (4), the hydrophilic layer (3) and the upper supporting layer (1) and the lower supporting layer (2) are all fixed through a layer of double faced adhesive tape (6) by the difference of widths.
2. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the width of the diffusion layer (4) is 3-4mm larger than the width of the reaction layer (5), the bottom surface of the diffusion layer (4) is tightly pressed and connected with the surface of the reaction layer (5), and two sides of the diffusion layer (4) extend out of two sides of the surface of the reaction layer (5) and are then adhered to the surface of the double faced adhesive tape (6).
3. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the width of the hydrophilic layer (3) is 3-4mm larger than the width of the diffusion layer (4), the width of the hydrophilic layer (3) is smaller than the width of the double faced adhesive tape (6) by 4-5mm, the bottom surface of the hydrophilic layer (3) is tightly pressed and connected with the surface of the diffusion layer (4), and two side sides of the hydrophilic layer (3) extend out of two side sides of the surface of the diffusion layer (4) and are then adhered to the surface of the double faced adhesive tape (6).
4. The dry chemical reagent strip for simultaneously quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the widths of the upper supporting layer (1) and the lower supporting layer (2) are the same, the middle part of the surface of the upper supporting layer (1) is provided with a sample inlet (11), and two sides of the upper supporting layer (1) extend out of the surface of the hydrophilic layer (3) and are adhered to the surface of the double faced adhesive tape (6).
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| CN111189899A (en) * | 2020-01-10 | 2020-05-22 | 杭州联晟生物科技有限公司 | Test strip for detecting creatinine by electrochemical method and preparation method thereof |
| CN113533312A (en) * | 2020-04-20 | 2021-10-22 | 杭州微策生物技术股份有限公司 | Photochemical POCT (point of care testing) all-in-one test card |
| CN113740327B (en) * | 2020-05-29 | 2023-03-03 | 北京京东方健康科技有限公司 | Reaction test paper, detection chip and detection system |
| CN113075142B (en) * | 2021-03-31 | 2023-10-03 | 复星诊断科技(长沙)有限公司 | Creatinine test strip and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103630535A (en) * | 2013-12-03 | 2014-03-12 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining content of human blood albumin |
| CN204116354U (en) * | 2014-07-30 | 2015-01-21 | 谢延科 | Blood five test paper |
| CN104316700A (en) * | 2014-10-11 | 2015-01-28 | 北京华益精点生物技术有限公司 | Preparation method of milk product multi-parameter quick detection card |
| CN210775230U (en) * | 2019-08-29 | 2020-06-16 | 安徽九陆生物科技有限公司 | Dry chemical reagent strip capable of simultaneously and quantitatively detecting breast milk multi-parameter |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6524864B2 (en) * | 2000-12-28 | 2003-02-25 | Aurora L. Fernandez Decastro | Test strip for simultaneous detection of a plurality of analytes |
| DE10346417A1 (en) * | 2003-10-07 | 2005-06-02 | Roche Diagnostics Gmbh | Analytical test element comprising a network for forming a capillary channel |
| KR102188654B1 (en) * | 2014-05-22 | 2020-12-08 | (주) 미코바이오메드 | Sensor strip and hemoglobin density measuring device using the sensor strip |
| CN106282312A (en) * | 2016-08-19 | 2017-01-04 | 基蛋生物科技股份有限公司 | A kind of dry chemistry bigeminy reagent strip for detecting glutamic oxaloacetic transaminase, GOT and glutamate pyruvate transaminase simultaneously |
| CN207457255U (en) * | 2017-08-30 | 2018-06-05 | 上海妙道生物科技有限公司 | Dry chemical scrip that is a kind of while detecting blood fat three |
| CN108374237A (en) * | 2017-12-23 | 2018-08-07 | 招汉 | A kind of dry-touch type transparent liquid non-woven fabrics and its manufacturing method |
-
2019
- 2019-08-29 CN CN201910808823.XA patent/CN110412027B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103630535A (en) * | 2013-12-03 | 2014-03-12 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining content of human blood albumin |
| CN204116354U (en) * | 2014-07-30 | 2015-01-21 | 谢延科 | Blood five test paper |
| CN104316700A (en) * | 2014-10-11 | 2015-01-28 | 北京华益精点生物技术有限公司 | Preparation method of milk product multi-parameter quick detection card |
| CN210775230U (en) * | 2019-08-29 | 2020-06-16 | 安徽九陆生物科技有限公司 | Dry chemical reagent strip capable of simultaneously and quantitatively detecting breast milk multi-parameter |
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