CN110412014A - Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection - Google Patents
Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection Download PDFInfo
- Publication number
- CN110412014A CN110412014A CN201910799903.3A CN201910799903A CN110412014A CN 110412014 A CN110412014 A CN 110412014A CN 201910799903 A CN201910799903 A CN 201910799903A CN 110412014 A CN110412014 A CN 110412014A
- Authority
- CN
- China
- Prior art keywords
- solution
- concentration
- added
- paper chip
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The preparation method of the good Surface enhanced Raman scattering paper base sensor of at low cost, high sensitivity that the invention discloses one kind, stability and the detection for being successfully used for tumor markers.By wax printing technique in paper chip preparation work area, and in workspace growth in situ good biocompatibility and the porous gold nanoparticle of Raman signal can be enhanced, it is embedded among gold and silver core-shell nanometer rod using by organic signaling molecule, greatly reduce the interference of external environment, stronger Raman signal and low-down background noise are generated, to realize the accurate detection to tumor markers.
Description
Technical field
The present invention relates to a kind of Surface enhanced Raman scattering detection technique fields, and more specifically one kind is to be suitable for drawing
The building of the micro-fluidic paper chip laboratory technique platform of graceful scattering analysis.
Background technique
Cancer is one of three big killers of human health, seriously threatens the health of the mankind.Wherein prostate cancer is
The malignant disease for seriously threatening men's health, the disease incidence in China show an increasing trend year by year, due to the disease incidence initial stage
Clinical symptoms are unobvious, and optimal treatment period is often had already passed by after manifest symptom occurs in patient, therefore find one kind and exist
Disease incidence initial stage feasible inspection method is the major issue for needing to solve at present.
Prostate epithelial cell can secrete prostate-specific antigen, it is considered as that the early stage of prostate cancer is examined
Break tumor markers the most suitable.Although the detection to prostate-specific antigen may be implemented now with many methods, than
Such as electrochemistry, fluorescence, electrogenerated chemiluminescence, colorimetric method, but inexpensive, high sensitivity, easy to operate and stability are good
Detection technique be still challenge.Paper chip has the characteristics that cheap, easy mass production, disposability, and
Also there is ideal biocompatibility, therefore obtained very extensive concern.The fibre structure of paper chip net distribution is also
Noble metal seed provides a good absorption environment, is conducive to the growth of a large amount of and continuous noble metal nanometer material.Table
Face enhancing Raman scattering has the advantages that many uniquenesses, for example, high sensitivity, unique spectral fingerprint, detect it is quick, lossless
Deng.With the fast development of Surface enhanced Raman scattering technology, for its signal enhancing mechanism research also by extensive discussions.It is existing
Mainly it is intended to two aspects in the research for Surface enhanced Raman scattering, is on the one hand expensive using the nanoscale of different structure
Metal particle arrays obtain the hot spot of more Electromagnetic enhancements；It on the other hand is then the selection of composite material, by Chemical enhancement machine
System is organically combined with Electromagnetic enhancement mechanism, improves Surface enhanced Raman scattering performance.The connection of paper chip and Raman detection technology
A very effective platform is provided with to construct biosensor inexpensive, easy to operate, that stability is good.
In order to further increase the sensitivity of prostate-specific antigen detection, signal amplification strategy becomes to be even more important.
We grow the gold nano-material of good biocompatibility, good conductivity on micro-fluidic paper base platform, not only considerably increase paper
The effective ratio area of chip provides more active sites for bio-ligand, and can greatly enhance Ramam effect, plays
The effect of signal amplification.In addition, organic signaling molecule is embedded among gold and silver core-shell nanometer rod, the external world can be greatly reduced
Interference, therefore can produce stronger Raman signal and low-down background noise.Based on above micro-fluidic constructed by us
Analysis device can be realized good signal amplification, and then realize the highly sensitive detection to antigen.
Summary of the invention
It is an object of the invention to make a kind of inexpensive, micro-fluidic paper base that easy to operate and stability is good analysis to set
It is standby, the highly sensitive detection of the prostate-specific antigen for low concentration.
In order to solve the above-mentioned technical problem, the present invention is micro-fluidic by constructing a kind of novel Surface enhanced Raman scattering
For paper base biosensor come what is realized, the preparation step of the sensor is as follows:
(1) the close and distant water area (attached drawing 1) of Adobe illustrator CS4 software design paper chip is used on computers；
The design that close and distant water area is carried out to paper chip, it is characterised in that designed pattern has white and green two face
Color region, wherein white area, that is, working region diameter dimension is 6 mm；
(2) No. 2 chromatographic papers are cut into A4 paper size, then use the pattern designed in wax printer printing step (1), with
Afterwards, the paper chip that wax prints is put into baking in baking oven melts wax, forms hydrophobic wall, and the part for not printing wax is hydrophilic work
Make area；
(3) porous gold nanoparticle is grown in hydrophilic working region, obtains the workspace of gold nanoparticle functionalization, is defined as
AuNPs/PWE；
It is described to grow porous gold nanoparticle in hydrophilic working region, AuNPs/PWE is obtained, it is characterized in that the following steps are included:
Gold seeds solution is synthesized first: measuring 80 mL deionized waters in three-necked flask, and 0.8 mL chlorine gold is added after being heated to 90 °C
Acid, is heated to 96 °C for solution and keeps 1 min, and the sodium citrate solution that 2.5-3.0 mL mass fraction is 1% is then added,
Continue to stir 15 min, cooled to room temperature；Secondly porous gold nanoparticle is grown in paper chip working region: will be freshly prepared
Gold seeds solution be added drop-wise to the hydrophilic workspace of paper chip, naturally dry, which is repeated 3 times, and is by freshly prepared concentration
The chlorauric acid solution Quick uniform that the hydroxylamine hydrochloride solution and mass fraction of 200 mM is 1% mixes, and is then added drop-wise to paper chip work
Make region and be incubated at room temperature 40 min, rinses paper chip using secondary water, naturally dry, repeats the above process one at room temperature
It is secondary, the growth of porous gold nanoparticle is completed, AuNPs/PWE is obtained；
(4) peptide chain for being modified with biotin is fixed on to the working region of step (3) obtained paper chip, then uses buffer solution
After cleaning, non-specific adsorption sites are reduced with sulfydryls hexanol；
The fixation of the peptide chain and sulfydryls hexanol block active site, comprising the following steps: by 60 μ L concentration are 0.01 mM
The peptide chain for being modified with biotin be added drop-wise to AuNPs/PWE, 12 h are incubated under 4 °C, after being cleaned with phosphate buffer, will
The sulfydryls hexanol that 60 μ L mass fractions are 1%, which is added in paper chip, is incubated for 1 h, to reduce non-specific adsorption sites；
(5) certain density prostate-specific antigen solution is added drop-wise to the surface of step (4) resulting paper chip, 37 °C
The lower cutting for being incubated for 40 min and carrying out peptide chain；
(6) Streptavidin will be modified with, be defined as SA, surface-enhanced Raman scattering probe be added drop-wise to complete peptide cleavage paper
On chip, it is incubated for 2 h under 37 °C, introduces probe on paper chip surface；
The surface in paper chip introduces probe, comprising the following steps: the preparation process of probe first is divided into four steps, the first step
It prepares seed solution: weighing 0.7289 g dodecyl trimethyl ammonium bromide and be dissolved in 10 mL water, under magnetic stirring, according to
The tetrahydro boron sodium that the secondary concentration that gold chloride and 1.2 mL ice that 10 mL concentration are 0.0005 M is added is 0.01 M, continues magnetic force
Seed solution is obtained after stirring 2 min；Second step prepares growth-promoting media: at room temperature, the dodecyl three for being 0.2 M by 5 mL concentration
The AgNO that methyl bromide ammonium salt solution and 0.2 mL concentration are 0.004 M3Solution mixing, it is 0.001 M that 5 mL concentration, which are then added,
Chlorauric acid solution, after being slowly stirred, be added 70 μ L concentration be 0.0788 M ascorbic acid, the color of growth-promoting media slowly by
Buff becomes colorless；Third step prepares gold nanorods, is defined as Au NRs: under 30 °C, 10-20 μ L seed solution quilt
It is added in above-mentioned growth-promoting media, reacts 40 min, obtain Au NRs for several times with secondary water washing；4th step, prepares surface enhanced
Raman scattering probe: by 700-1000 μ L 4- mercaptobenzoic acid, being defined as 4-MBA, is added in 1 mL Au NRs solution,
Obtained mixed liquor places 6 h at room temperature, solution is centrifuged to 5 min under 12000 rpm to remove extra 4-MBA, obtained
To solid be easily re-dispersed in 1 mL secondary water, by this dispersion liquid be added under magnetic stirring 5 mL concentration be 0.04 M
Dodecyl trimethyl ammonium bromide solution in, then, be separately added into 150 μ L concentration be 0.01 M AgNO3Solution, 130 μ
The NaOH solution that the ascorbic acid solution and 290 μ L concentration that L concentration is 0.1 M are 0.1 M, 12000 after 1 h of magnetic agitation
It is centrifuged 5 min under rpm revolving speed, is cleaned once with secondary water, precipitating is distributed in 1 mL water；Secondly the spy of SA modification is prepared
Needle: 50-200 μ L concentration is that the SA of 1 mg/mL is added into the above-mentioned probe solution of 500 μ L, and mixture is in 4 °C of conditions
12 h of lower concussion are cleaned with the phosphate buffer of pH 7.4 and are removed extra SA, obtain the probe of SA modification；Finally, SA is repaired
The probe of decorations, which is easily re-dispersed, is incubated for 4 in the phosphate buffer that 500 μ L contain the bovine serum albumin(BSA) that mass fraction is 1%
The 60 μ L surface-enhanced Raman scattering probe for being modified with SA is then added drop-wise in step (5) resulting paper chip, 37 °C by h
2 h of lower incubation are thoroughly washed paper chip 3 times using secondary water, dry 50 min at room temperature；
(7) fine surfaces enhancing Raman scattering measurement: step (6) obtained paper chip is placed on Raman detection platform, complete
At measurement, the relation curve between raman scattering intensity and prostate-specific antigen concentration is drawn, realizes prostate-specific antigen
Accurate detection.
Beneficial effects of the present invention
(1) the uniform and gold nanoparticle growth in situ with porous structure of roughness is optimized significantly in flexible paper chip
Raman scattering performance, it is not only easy to operate, easy to carry, and also paper chip has certain biological degradability, avoids to ring
The pollution in border.
(2) organic molecule is embedded among gold and silver core-shell nanometer rod, can produce hot spot in more metals, prevents week
The interference in collarette border, so as to obtain bigger Raman signal and lower background noise.
(3) stability in use is higher and is easier to the polypeptide chain synthesized on a molecular scale to replace traditional antibody, can be with
Greatly improve the stability of sensor.
Detailed description of the invention
Fig. 1: the hydrophobic wax print pattern of paper chip.
Specific embodiment
For a better understanding of the present invention, below with reference to the embodiment content that the present invention is further explained, but it is of the invention
Content is not limited solely to following implementation.
Embodiment 1
It a kind of preparation of the paper base sensor based on Surface enhanced Raman scattering technology and its is detected in prostate-specific antigen
In application, it is characterized in that the following steps are included:
(1) the close and distant water area of Adobe illustrator CS4 software design paper chip is used on computers；
The design that close and distant water area is carried out to paper chip, it is characterised in that designed pattern has white and green two face
Color region, wherein white area, that is, working region diameter dimension is 6 mm；
(2) No. 2 chromatographic papers are cut into A4 paper size, then use the pattern designed in wax printer printing step (1), with
Afterwards, the paper chip that wax prints is put into and toasts 3 min in 150 °C of constant temperature of baking oven and melts wax, formed hydrophobic wall, do not beat
The part for printing wax is hydrophilic area；
(3) porous gold nanoparticle is grown in hydrophilic working region, obtains the workspace of gold nanoparticle functionalization, is defined as
AuNPs/PWE；
It is described to grow porous gold nanoparticle in hydrophilic working region, AuNPs/PWE is obtained, it is characterized in that the following steps are included:
Gold seeds solution is synthesized first: measuring 80 mL deionized waters in three-necked flask, and 0.8 mL chlorine gold is added after being heated to 90 °C
Acid, is heated to 96 °C for solution and keeps 1 min, and the sodium citrate solution that 2.8 mL mass fractions are 1% is then added, continues
Stir 15 min, cooled to room temperature；Secondly porous gold nanoparticle is grown in paper chip working region: by freshly prepd gold
Seed solution is added drop-wise to the hydrophilic workspace of paper chip, naturally dry, which is repeated 3 times.It is 200 by freshly prepared concentration
The chlorauric acid solution Quick uniform that the hydroxylamine hydrochloride solution and mass fraction of mM is 1% mixes, and is then added drop-wise to paper chip workspace
Domain is simultaneously incubated at room temperature 40 min, rinses paper chip using secondary water, at room temperature naturally dry, repeats the above process once,
The growth for completing porous gold nanoparticle, obtains AuNPs/PWE；
(4) peptide chain for being modified with biotin is fixed on to the working region of step (3) obtained paper chip, then uses buffer solution
After cleaning, non-specific adsorption sites are reduced with sulfydryls hexanol；
The fixation of the peptide chain and sulfydryls hexanol block active site, comprising the following steps: by 60 μ L concentration are 0.01 mM
The peptide chain for being modified with biotin be added drop-wise to AuNPs/PWE, 12 h are incubated under 4 °C, polypeptide is made to be fixed on electricity by Au-S key
Pole surface after being cleaned with phosphate buffer, the sulfydryls hexanol that 60 μ L mass fractions are 1% is added in paper chip and is incubated for 1
H, to reduce non-specific adsorption sites；
(5) the certain density prostate-specific antigen solution of 40 μ L is added drop-wise to the surface of step (4) resulting paper chip,
The cutting that 40 min carry out peptide chain is incubated under 37 °C；
(6) Streptavidin will be modified with, be defined as SA, surface-enhanced Raman scattering probe be added drop-wise to complete peptide cleavage paper
On chip, it is incubated for 2 h under 37 °C, introduces probe on paper chip surface；
The surface in paper chip introduces probe, comprising the following steps: the preparation process of probe first is divided into four steps, the first step
It prepares seed solution: weighing 0.7289 g dodecyl trimethyl ammonium bromide and be dissolved in 10 mL water, under magnetic stirring, according to
The tetrahydro boron sodium that the secondary concentration that gold chloride and 1.2 mL ice that 10 mL concentration are 0.0005 M is added is 0.01 M, continues magnetic force
Seed solution is obtained after stirring 2 min；Second step prepares growth-promoting media: at room temperature, the dodecyl three for being 0.2 M by 5 mL concentration
The AgNO that methyl bromide ammonium salt solution and concentration are 0.004 M30.2 mL of solution mixing, it is 0.001 M that 5 mL concentration, which are then added,
Chlorauric acid solution, after being slowly stirred, be added 70 μ L concentration be 0.0788 M ascorbic acid, the color of growth-promoting media slowly by
Buff becomes colorless；Third step prepares gold nanorods, is defined as Au NRs: under 30 °C, 12 μ L seed solutions are added into
Into above-mentioned growth-promoting media, 40 min are reacted, obtain Au NRs for several times with secondary water washing；4th step, prepares surface-enhanced Raman
Scattering probe: by 800 μ L 4- mercaptobenzoic acids, being defined as 4-MBA, be added in 1 mL Au NRs solution, obtained mixing
Liquid places 6 h at room temperature, and solution is centrifuged to 5 min under 12000 rpm to remove extra 4-MBA, obtained solid quilt
Again it is dispersed in 1 mL secondary water, this dispersion liquid is added to ten that 5 mL concentration are 0.04 M under 30 °C of magnetic agitations
In dialkyl group trimethylammonium bromide solution, then, it is separately added into the AgNO that 150 μ L concentration are 0.01 M3, 130 μ L concentration are
The ascorbic acid of 0.1 M and 290 μ L concentration are the NaOH solution of 0.1 M, after 1 h of magnetic agitation under 12000 rpm revolving speeds from
5 min of the heart is cleaned once with secondary water, and precipitating is distributed in 1 mL water；Secondly prepare the probe of SA modification: 100 μ L's is dense
Degree is that the SA of 1 mg/mL is added into the above-mentioned probe solution of 500 μ L, and mixture shakes 12 h under the conditions of 4 °C, uses pH
7.4 phosphate buffer cleaning removes extra SA, obtains the probe of SA modification；Finally, the probe of SA modification is divided again
It is dispersed in 500 μ L and contains 4 h in the phosphate buffer for the bovine serum albumin(BSA) that mass fraction is 1%, then, 60 μ L are modified
There is the surface-enhanced Raman scattering probe of SA to be added drop-wise in step (5) resulting paper chip, 2 h is incubated under 37 °C, use is secondary
Water thoroughly washs paper chip 3 times, dries 50 min at room temperature；
(7) fine surfaces enhancing Raman scattering measurement: step (6) obtained paper chip is placed on Raman detection platform, complete
At measurement, the relation curve between raman scattering intensity and prostate-specific antigen concentration is drawn, realizes prostate-specific antigen
Accurate detection.
Sequence table
<110>University Of Ji'nan
<120>application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection
<130> 2019
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 12
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 1
Cys Glu His Ser Ser Lys Leu Gln Leu Ala Lys Ser
1 5 10
Claims (1)
1. application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection, feature the following steps are included:
1.1 use the close and distant water area of Adobe illustrator CS4 software design paper chip on computers；
The design that close and distant water area is carried out to paper chip, it is characterised in that designed pattern has white and green two face
Color region, wherein white area, that is, working region diameter dimension is 6 mm；
No. 2 chromatographic papers are cut into A4 paper size by 1.2, then use the pattern designed in wax printer printing step 1.1, with
Afterwards, the paper chip that wax prints is put into baking in baking oven melts wax, forms hydrophobic wall, and the part for not printing wax is hydrophilic work
Make area；
1.3 grow porous gold nanoparticle in hydrophilic working region, obtain the workspace of gold nanoparticle functionalization, are defined as
AuNPs/PWE；
It is described to grow porous gold nanoparticle in hydrophilic working region, AuNPs/PWE is obtained, it is characterized in that the following steps are included:
Gold seeds solution is synthesized first: measuring 80 mL deionized waters in three-necked flask, and 0.8 mL chlorine gold is added after being heated to 90 °C
Acid, is heated to 96 °C for solution and keeps 1 min, and the sodium citrate solution that 2.5-3.0 mL mass fraction is 1% is then added,
Continue to stir 15 min, cooled to room temperature；Secondly porous gold nanoparticle is grown in paper chip working region: will be freshly prepared
Gold seeds solution be added drop-wise to the hydrophilic workspace of paper chip, naturally dry, which is repeated 3 times, and is by freshly prepared concentration
The chlorauric acid solution Quick uniform that the hydroxylamine hydrochloride solution and mass fraction of 200 mM is 1% mixes, and is then added drop-wise to paper chip work
Make region and be incubated at room temperature 40 min, rinses paper chip using secondary water, naturally dry, repeats the above process one at room temperature
It is secondary, the growth of porous gold nanoparticle is completed, AuNPs/PWE is obtained；
1.4 are fixed on the peptide chain for being modified with biotin the working region of the obtained paper chip of step 1.3, then use buffer solution
After cleaning, non-specific adsorption sites are reduced with sulfydryls hexanol；
The fixation of the peptide chain and sulfydryls hexanol block active site, comprising the following steps: by 60 μ L concentration are 0.01 mM
The peptide chain for being modified with biotin be added drop-wise to AuNPs/PWE, 12 h are incubated under 4 °C, after being cleaned with phosphate buffer, will
The sulfydryls hexanol that 60 μ L mass fractions are 1%, which is added in paper chip, is incubated for 1 h, to reduce non-specific adsorption sites；
1.5 are added drop-wise to certain density prostate-specific antigen solution the surface of the resulting paper chip of step 1.4, and 37 °C
The lower cutting for being incubated for 40 min and carrying out peptide chain；
1.6 are added drop-wise to the surface-enhanced Raman scattering probe for being modified with Streptavidin and being defined as SA the core for completing peptide cleavage
On piece is incubated for 2 h under 37 °C, introduces probe on paper chip surface；
The surface in paper chip introduces probe, comprising the following steps: the preparation process of probe first is divided into four steps, the first step
It prepares seed solution: weighing 0.7289 g dodecyl trimethyl ammonium bromide and be dissolved in 10 mL water, under magnetic stirring, according to
The tetrahydro boron sodium that the secondary concentration that gold chloride and 1.2 mL ice that 10 mL concentration are 0.0005 M is added is 0.01 M, continues magnetic force
Seed solution is obtained after stirring 2 min；Second step prepares growth-promoting media: at room temperature, the dodecyl three for being 0.2 M by 5 mL concentration
The AgNO that methyl bromide ammonium salt solution and 0.2 mL concentration are 0.004 M3Solution mixing, it is 0.001 M that 5 mL concentration, which are then added,
Chlorauric acid solution, after being slowly stirred, be added 70 μ L concentration be 0.0788 M ascorbic acid, the color of growth-promoting media slowly by
Buff becomes colorless；Third step prepares gold nanorods, is defined as Au NRs: under 30 °C, 10-20 μ L seed solution quilt
It is added in above-mentioned growth-promoting media, reacts 40 min, obtain Au NRs for several times with secondary water washing；4th step, prepares surface enhanced
Raman scattering probe: by 700-1000 μ L 4- mercaptobenzoic acid, being defined as 4-MBA, is added in 1 mL Au NRs solution,
Obtained mixed liquor places 6 h at room temperature, solution is centrifuged to 5 min under 12000 rpm to remove extra 4-MBA, obtained
To solid be easily re-dispersed in 1 mL secondary water, by this dispersion liquid be added under magnetic stirring 5 mL concentration be 0.04 M
Dodecyl trimethyl ammonium bromide solution in, then, be separately added into 150 μ L concentration be 0.01 M AgNO3Solution, 130 μ
The NaOH solution that the ascorbic acid solution and 290 μ L concentration that L concentration is 0.1 M are 0.1 M, 12000 after 1 h of magnetic agitation
It is centrifuged 5 min under rpm revolving speed, is cleaned once with secondary water, precipitating is distributed in 1 mL water；Secondly the spy of SA modification is prepared
Needle: the concentration of 50-200 μ L is that the SA of 1 mg/mL is added into the above-mentioned probe solution of 500 μ L, and mixture is in 4 °C of items
12 h are shaken under part, is cleaned with the phosphate buffer of pH 7.4 and removes extra SA, obtain the probe of SA modification；Finally, SA
The probe of modification is easily re-dispersed incubates in the phosphate buffer that 500 μ L contain the bovine serum albumin(BSA) that mass fraction is 1%
4 h are educated, then, the 60 μ L surface-enhanced Raman scattering probe for being modified with SA is added drop-wise in the resulting paper chip of step 1.5,
It is incubated for 2 h under 37 °C, is thoroughly washed using secondary water paper chip 3 times, dries 50 min at room temperature；
The enhancing Raman scattering measurement of 1.7 fine surfaces: the obtained paper chip of step 1.6 is placed on Raman detection platform, complete
At measurement, the relation curve between raman scattering intensity and prostate-specific antigen concentration is drawn, realizes prostate-specific antigen
Accurate detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910799903.3A CN110412014A (en) | 2019-08-28 | 2019-08-28 | Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910799903.3A CN110412014A (en) | 2019-08-28 | 2019-08-28 | Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110412014A true CN110412014A (en) | 2019-11-05 |
Family
ID=68369360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910799903.3A Pending CN110412014A (en) | 2019-08-28 | 2019-08-28 | Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110412014A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702665A (en) * | 2019-11-14 | 2020-01-17 | 济南大学 | Preparation of paper-based coupling enhanced Raman sensor and application of paper-based coupling enhanced Raman sensor in okadaic acid detection |
| CN110779905A (en) * | 2019-11-19 | 2020-02-11 | 中国人民解放军军事科学院军事医学研究院 | Gold nano-labeled test strip based on surface-enhanced Raman scattering, preparation method and use method |
| CN110887828A (en) * | 2019-12-04 | 2020-03-17 | 济南大学 | SERS mechanism based on Glass/AuNSts-SLG for detecting rhodamine B in solution |
| CN113125411A (en) * | 2021-04-29 | 2021-07-16 | 江苏大学 | SERS (surface enhanced Raman Scattering) probe for detecting patulin as well as preparation method and application thereof |
| CN113967489A (en) * | 2021-10-21 | 2022-01-25 | 中国热带农业科学院分析测试中心 | Methyl parathion microfluidic paper-based detection chip, preparation and detection method and application |
| CN114878541A (en) * | 2022-05-16 | 2022-08-09 | 深圳技术大学 | High-sensitivity Raman enhancement substrate for rapid detection and preparation method thereof |
| CN115404279A (en) * | 2022-02-18 | 2022-11-29 | 天津科技大学 | Method for detecting pathogenic bacteria by combination of CRISPR/Cas system and microfluidic paper analysis device based on SERS and application |
| CN115586168A (en) * | 2022-09-16 | 2023-01-10 | 中国人民解放军国防科技大学 | Explosive RDX detection method based on surface enhanced Raman scattering |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130060009A (en) * | 2011-11-29 | 2013-06-07 | 삼성전자주식회사 | Device for detecting and separating target biomolecules and method for detecting and separating target biomolecules using the same |
| CN105115958A (en) * | 2015-09-17 | 2015-12-02 | 苏州大学 | Surface-enhanced Raman substrate based on large-sized metal nanoparticles and preparation method thereof |
| CN105924478A (en) * | 2016-05-24 | 2016-09-07 | 济南大学 | Preparation method of three-dimensional paper-based metal organic framework |
| CN106924764A (en) * | 2017-04-24 | 2017-07-07 | 南京邮电大学 | A kind of preparation method and applications of the feature light sonic probe based on gold nano grain assembling |
| CN107328753A (en) * | 2017-07-14 | 2017-11-07 | 厦门稀土材料研究所 | The nanoparticle surface enhancing Raman spectrum quantitative analysis method of embedded internal standard molecule |
| CN107741415A (en) * | 2017-08-30 | 2018-02-27 | 杨蕾 | One kind is based on magnetic Nano assembly double check small molecule and method of protein |
| CN109001176A (en) * | 2018-06-14 | 2018-12-14 | 福建师范大学 | A kind of preparation method of the SERS substrate of Au@Ag nanoparticle and method using substrate detection glucose |
| CN109564223A (en) * | 2016-09-06 | 2019-04-02 | 富士瑞必欧株式会社 | The measuring method and measurement reagent of tumor marker |
-
2019
- 2019-08-28 CN CN201910799903.3A patent/CN110412014A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130060009A (en) * | 2011-11-29 | 2013-06-07 | 삼성전자주식회사 | Device for detecting and separating target biomolecules and method for detecting and separating target biomolecules using the same |
| CN105115958A (en) * | 2015-09-17 | 2015-12-02 | 苏州大学 | Surface-enhanced Raman substrate based on large-sized metal nanoparticles and preparation method thereof |
| CN105924478A (en) * | 2016-05-24 | 2016-09-07 | 济南大学 | Preparation method of three-dimensional paper-based metal organic framework |
| CN109564223A (en) * | 2016-09-06 | 2019-04-02 | 富士瑞必欧株式会社 | The measuring method and measurement reagent of tumor marker |
| CN106924764A (en) * | 2017-04-24 | 2017-07-07 | 南京邮电大学 | A kind of preparation method and applications of the feature light sonic probe based on gold nano grain assembling |
| CN107328753A (en) * | 2017-07-14 | 2017-11-07 | 厦门稀土材料研究所 | The nanoparticle surface enhancing Raman spectrum quantitative analysis method of embedded internal standard molecule |
| CN107741415A (en) * | 2017-08-30 | 2018-02-27 | 杨蕾 | One kind is based on magnetic Nano assembly double check small molecule and method of protein |
| CN109001176A (en) * | 2018-06-14 | 2018-12-14 | 福建师范大学 | A kind of preparation method of the SERS substrate of Au@Ag nanoparticle and method using substrate detection glucose |
Non-Patent Citations (6)
| Title |
|---|
| CHEN LEI等: "Immune recognition construct plasmonic dimer for SERS-based bioassay", 《JOURNAL OF RAMAN SPECTROSCOPY》 * |
| LIANG ZHAOHENG等: "SERS-based cascade amplification bioassay protocol of miRNA-21 by using sandwich structure with biotin–streptavidin system", 《ANALYST》 * |
| WEN SHENGPING等: "Plasmonic Au nanostar Raman probes coupling with highly ordered TiO2-Au nanotube arrays as the reliable SERS sensing platform for chronic myeloid leukemia drug evaluation", 《BIOSENSORS AND BIOELECTRONICS》 * |
| YANG AN-QI等: "Rational design of Au nanorods assemblies for highly sensitive and selective SERS detection of prostate specific antigen", 《RSC ADVANCES》 * |
| ZHENG XIAOXIAO等: "Ultrasensitive Enzyme-free Biosensor by Coupling Cyclodextrin Functionalized Au Nanoparticles and High-Performance Au-Paper Electrode", 《ACS APPLIED MATERIALS & INTERFACES》 * |
| 封昭等: "基于金/银纳米三明治结构SERS特性的超灵敏前列腺特异性抗原检测", 《发光学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702665A (en) * | 2019-11-14 | 2020-01-17 | 济南大学 | Preparation of paper-based coupling enhanced Raman sensor and application of paper-based coupling enhanced Raman sensor in okadaic acid detection |
| CN110779905A (en) * | 2019-11-19 | 2020-02-11 | 中国人民解放军军事科学院军事医学研究院 | Gold nano-labeled test strip based on surface-enhanced Raman scattering, preparation method and use method |
| CN110887828A (en) * | 2019-12-04 | 2020-03-17 | 济南大学 | SERS mechanism based on Glass/AuNSts-SLG for detecting rhodamine B in solution |
| CN113125411A (en) * | 2021-04-29 | 2021-07-16 | 江苏大学 | SERS (surface enhanced Raman Scattering) probe for detecting patulin as well as preparation method and application thereof |
| CN113967489A (en) * | 2021-10-21 | 2022-01-25 | 中国热带农业科学院分析测试中心 | Methyl parathion microfluidic paper-based detection chip, preparation and detection method and application |
| CN115404279A (en) * | 2022-02-18 | 2022-11-29 | 天津科技大学 | Method for detecting pathogenic bacteria by combination of CRISPR/Cas system and microfluidic paper analysis device based on SERS and application |
| CN114878541A (en) * | 2022-05-16 | 2022-08-09 | 深圳技术大学 | High-sensitivity Raman enhancement substrate for rapid detection and preparation method thereof |
| CN115586168A (en) * | 2022-09-16 | 2023-01-10 | 中国人民解放军国防科技大学 | Explosive RDX detection method based on surface enhanced Raman scattering |
| CN115586168B (en) * | 2022-09-16 | 2024-05-14 | 中国人民解放军国防科技大学 | Explosive RDX detection method based on surface enhanced Raman scattering |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110412014A (en) | Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection | |
| Tang et al. | Plasmon-based colorimetric nanosensors for ultrasensitive molecular diagnostics | |
| CN103357886B (en) | A kind of preparation method of the noble metal nano cluster for fluorescent optical sensor | |
| Bosak et al. | Aptamer–gold nanoparticle conjugates for the colorimetric detection of arboviruses and vector mosquito species | |
| CN107478641A (en) | Liquid phase SERS sensor, its preparation method and its purposes for detection of nucleic acids | |
| CN113125403B (en) | Raman-fluorescence dual-mode detection method for calcium ions based on dual-mode nano probe | |
| CN106052872B (en) | A kind of terramycin SERS detection methods based on nano material self assembly | |
| CN111413385A (en) | Method for detecting GPC3 based on RGO-CS-Fc/Pt-Pd NPs nano composite material | |
| CN109085158B (en) | Used for cancer cell and H2O2Construction of paper-based sensors for detection | |
| CN107064118A (en) | A kind of construction method of the photic electrochemica biological sensor of paper substrate high flux | |
| CN106596942B (en) | A kind of construction method of interlayer type hepatitis b virus marker immunosensor and application | |
| Huang et al. | Label-free optical biosensor based on localized surface plasmon resonance of immobilized gold nanorods | |
| CN102608102A (en) | Specific detection method of human breast cancer cells MCF-7 based on surface-enhanced Raman spectroscopy | |
| CN102621125A (en) | Cell probe complex for detecting human breast cancer cells MCF (Michigan cancer foundation)-7 based on Raman spectrum and method for preparing cell probe complex | |
| CN104007264A (en) | Preparation method and detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization | |
| CN104977277A (en) | Nanometer vesica capable of detecting wild type p53 protein and variant p53 protein in cells simultaneously | |
| Tang et al. | LHRH-targeting surface-enhanced Raman scattering tags for the rapid detection of circulating tumor cells | |
| CN106526182B (en) | A kind of structure of paper substrate aptamer fluorescent optical sensor for fibrin ferment detection | |
| CN108469461A (en) | A kind of preparation method and application of interlayer type lung cancer marker electrochemical sensor | |
| CN108375612B (en) | A kind of method of composite nano materials Electrochemical Detection alpha-fetoprotein | |
| JP2007139556A (en) | New analysis method and kit | |
| CN107271519B (en) | A kind of preparation method and application of the immunosensor of the Sulfonated carbon nanotube based on load Rh@Pd nanodendrites | |
| CN106290332B (en) | A kind of magnetic porous silicon montmorillonite Composite nano material for Rapid Detection of Clenbuterol | |
| CN106645076A (en) | Total protein fluorescence test kit taking gold nanocluster as probe | |
| CN110031447A (en) | A kind of detection method that substrate being immunized using magnetic surface enhancing Raman |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191105 |