CN109001176A - A kind of preparation method of the SERS substrate of Au@Ag nanoparticle and method using substrate detection glucose - Google Patents
A kind of preparation method of the SERS substrate of Au@Ag nanoparticle and method using substrate detection glucose Download PDFInfo
- Publication number
- CN109001176A CN109001176A CN201810611514.9A CN201810611514A CN109001176A CN 109001176 A CN109001176 A CN 109001176A CN 201810611514 A CN201810611514 A CN 201810611514A CN 109001176 A CN109001176 A CN 109001176A
- Authority
- CN
- China
- Prior art keywords
- glucose
- concentration
- core
- solution
- sers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
Abstract
The invention discloses a kind of preparation methods of the SERS substrate of Au@Ag nanoparticle.Nano Au particle is made using reduction of sodium citrate gold chloride in this method, and 4- mercaptoaniline connects silver ion in modification, adds a certain proportion of ascorbic acid and sodium hydroxide restores silver-colored shell.Meanwhile the morphology of core-shell structure size is controllable, plasma resonance is realized in structure height coupling, so-called " hot spot " is formed inside core-shell structure, so that the 4- mercaptoaniline as internal standard molecule has very strong Raman signal.4- mercaptophenyl boronic acid is modified on this Au Ag core-shell structure, to capture glucose molecule, to realize the qualitative and quantitative detection of glucose.The present invention has the advantages that doing signal contrast using internal standard substance, detection sensitivity will not be improved by the interference of complex material in sample (such as urine), preparation cost is low, really realizes the qualitative and quantitative detection of glucose.
Description
Technical field
The invention belongs to the technical fields of detection, and in particular to a kind of preparation side of the SERS substrate of Au@Ag nanoparticle
Method and the method for detecting glucose using the substrate.
Background technique
It with carbohydrate metabolism disturbance is the incretion metabolism disease mainly showed that diabetes (diabetes), which are a kind of,.Nearly tens
Year, the number of global diabetic increases rapidly at an amazing speed, has become seriously affect compatriots' physical and mental health at present
Major public health problem, treatment there is no effect method at present, and can generate multiple complications, therefore early prevention seems outstanding
It is important.Plasma glucose is current diabetes uniquely reliable diagnosis index, and judges diabetic condition and control situation
Main foundation.
The currently used detection method of blood sugar test has: fasting plasma glucose detection, the inspection of postprandial 2 hours plasma glucoses
It surveys, venous plasma glucose detection etc., but all there are some disadvantages, if fasting blood-glucose is by time restriction, sensibility is low, is easy leakage
It examines；Postprandial blood sugar is unstable, poor reproducibility;And there is pain in blood sugar test, therefore we need one kind that can accomplish lossless inspection
It surveys while affected by environment small, reproducibility is strong, the glucose sensing approach of high sensitivity.
And Surface enhanced Raman scattering (Surface Enhanced Raman Scattering, SERS) technology can because of it
To reflect the structural information of molecule well, have many advantages, such as high sensitivity, high-resolution and fast reaction, was changing in recent years
It learns, development is very fast in terms of environment and the especially medical sensing detection of biology.However accuracy controlling and preparation SERS activity heat
Point, realizing large area, low cost and expeditiously preparing the high SERS active-substrate enhanced is still a difficulty in current research
Point.
A kind of glucose sensing approach based on Surface enhanced Raman scattering and bi-molecular probe of patent
(CN2016104501037), specifically disclose it is a kind of using gold and silver shell core nanometer rods as SERS active-substrate, and with primary Portugal
Above-mentioned SERS active-substrate in SERS active-substrate, is then soaked in by grape saccharide acceptor molecule (using 4- mercaptophenyl boronic acid) modification
In glucose solution, glucose is captured in SERS active-substrate；Then second level glucose is added into SERS active-substrate
Acceptor molecule (uses 4- cyanophenylboronic acid), and second level glucoreceptor molecular selection is covalently tied with glucose molecule in substrate
It closes, it is final to carry out SERS spectra test.Although the above method improves the sensitivity of glucose detection to a certain extent,
Glucose is detected using the substrate, method is complicated, needs to carry out the addition of 2 glucose molecule probes.And due to its use
Cyano group Raman signal it is weaker, glucose concentration range that can be detected is smaller, can only achieve 10-2 mol/L, this will not
Suitable for being detected to micro glucose.
Summary of the invention
To solve the above-mentioned problems, the present invention provides SERS substrate, preparation method and the benefits of a kind of Au@Ag nanoparticle
With the method for substrate detection glucose, the glucose SERS detection substrate based on Au@Ag nanoparticle is to utilize bimetallic core
Shell structure realizes plasma resonance, to form SERS activity hot spot, has made the intermediate internal standard substance 4- mercaptoaniline wrapped up both
Feature SERS signal is enhanced, and can be from the interference of detection environment.The 4- mercaptophenyl boronic acid energy modified outside core-shell structure simultaneously
Enough specific recognition glucose molecules, the strong sensitivity that ensure that the SERS substrate, specificity and signal homogeneity, it is another
Aspect simplifies the detecting step of glucose.
In order to achieve the object of the present invention, the present invention is by a kind of band internal standard substance --- Ag nanometers of the Au@of 4- mercaptoaniline
Particle is used as substrate, and glucose capture molecule 4- mercaptophenyl boronic acid is in modification to achieve the effect that specific detection glucose.
Method above-mentioned further include detect before choose five various concentrations glucose standard liquid reacted, using SERS into
Row detection；Simultaneously using the method that micro glucose is added, glucose in urine is detected.And the feature SERS that will be obtained
Spectrum carries out internal standardization processing to obtain the working curve of detection glucose, the detection for concentration of glucose in urine.
The bimetal nano core-shell structure being related in the invention, wherein nanogold nuclear diameter is 23-27nm, nano silver shell
For 5-10nm, centre is 4- mercaptoaniline, wherein with 5 × 10-4The concentration modification of mol/L is best.
Detection method includes the following steps for glucose in a kind of urine:
1) 90mL distilled water is added into the chlorauric acid solution that 10mL concentration is 2.4mmol/L, is heated to boiling, 1% lemon is added
It is centrifugated after lemon acid sodium solution reaction 15min, sediment is washed with distilled water and dehydrated alcohol, and 80 DEG C dry to obtain nanogold.
2) nanogold of 15 μ L -40 μ L is added to the 4- mercaptoaniline solution reaction 12- of 0.05mmol/L-5mmol/L
After 20 is small, centrifuge washing.
3) it is sequentially added by volume for 0.5-0.8:20-35:40-55 in the nanogold prepared to 20mL step (2)
The sodium hydroxide solution of the silver nitrate solution of 100mmol/L, 100mmol/L ascorbic acid and 100mmol/L reacts 4- under room temperature
6 hours, to go out nano silver shell in nanogold surface reduction, centrifuge washing, taking lower layer's solution was Au Ag nano-core-shell structure
Colloid；
4) the 4- sulfydryl that 5 μ L-30 μ L concentration are 1mmol/L is added in the Au@Ag nano-core-shell structure colloid for taking 20mL to prepare
Phenyl boric acid solution reacts under stirring condition 2-3 hour, and reaction temperature is 60 DEG C -80 DEG C, and centrifuge washing is dried, and obtains Au@Ag and receives
The SERS substrate of rice corpuscles.
5) Glucose standards of glucose SERS the detection substrate and configured 5-6 various concentration gradient of preparation are molten
Liquid carries out after being mixed 0.5-1 hours, and drop on the cover slip, the inspection of Raman signal is carried out using Reinshaw Raman spectrometer
It surveys, obtains the SERS spectrogram of corresponding concentration.
6) spectrogram is handled, takes 1388cm-1Place's wave crest is normalized, further according to 1077,1177,1584cm-1
The intensity of characteristic peak obtains corresponding working curve with the relationship of concentration of glucose at three.
7) it detects unknown sample concentration: the glucose SERS detection substrate of the sample preparation with measurement of unknown concentration is mixed
It closes, is then tested using laser Raman spectrometer, and carry out spectral strength normalized and obtain the grape of unknown concentration
Sugared Surface enhanced Raman spectroscopy spectrogram；The concentration of glucose is calculated by formula.
Choose 1177cm-1The working curve at place is calculation template, takes the glucose solution of corresponding amount to be added to unprocessed
Healthy People urine in, similarly react 0.5-1 hours with glucose SERS substrate, survey SERS spectra, and draw
Curve compares；It is detected using the present invention, discovery is linear to coincide substantially, illustrates this detection method by object complicated in urine
The interference of matter is smaller, it is seen that the features such as glucose SERS substrate of the invention has high sensitivity, and specificity is good, and reproducibility is strong.
Detailed description of the invention
Fig. 1 is that the glucose SERS of Au@Ag nano-core-shell structure detects the preparation process of substrate.
Fig. 2 is the transmission electron microscope picture of the Au@Ag nano-core-shell structure of embodiment 4.
Fig. 3 is the transmission electron microscope picture of the Au@Ag nano-core-shell structure colloid of embodiment 1.
Fig. 4 is the transmission electron microscope picture of the Au@Ag nano-core-shell structure colloid of embodiment 2.
Fig. 5 is the transmission electron microscope picture of the Au@Ag nano-core-shell structure colloid of embodiment 3
Fig. 6 is that the glucose SERS of the Au@Ag nano-core-shell structure prepared using embodiment 4 detects substrate detection various concentration
The SERS spectra of glucose standards solution.
The 1177cm that Fig. 7 is made for the SERS spectra using Fig. 4-1The work that the peak intensity at place changes with concentration of glucose
Make curve graph.
Fig. 8 is that the glucose SERS of the Au@Ag nano-core-shell structure prepared using embodiment 4 is detected in substrate detection urine
The SERS spectra of the glucose of various concentration.
The 1177cm that Fig. 9 is made for the SERS spectra using Fig. 8-1The work that the peak intensity at place changes with concentration of glucose
Make curve graph.
Specific embodiment
In order to better understand the present invention, below by embodiment to the present invention into further explanation, embodiment is served only for
It explains the present invention, any restriction can't be constituted to the present invention.
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Embodiment 1
The detection method of glucose in a kind of urine, comprising the following steps:
1) to 10mL, 90mL distilled water is added in the chlorauric acid solution of 2.4mmol/L, is heated to boiling, 1% citric acid is added
Sodium solution reaction 15min obtains nanogold.
2) nanogold is small with the 4- mercaptoaniline solution reaction at least 12 of various concentration (0.05mmol/L, 20 μ L) respectively
When, it is then centrifuged for washing.
3) example (0.6:25:50) sequentially adds 100mmol/L nitre by volume in the nanogold prepared to 20mL step (2)
Sour silver solution, 100mmol/L ascorbic acid and 100mmol/L sodium hydroxide solution react 4 hours under room temperature, restore nanometer
Silver-colored shell obtains the particle of Au Ag nano-core-shell structure required for us, and centrifuge washing, taking lower layer's solution is Ag nanometers of Au
Core-shell structure colloid.
4) the 4- sulfydryl benzene boron of (1mmol/L, 10 μ L) is added in the Au@Ag nano-core-shell structure colloid for taking 20mL to prepare
Acid solution reacts 2 hours, and control temperature is at 60 DEG C or so, centrifuge washing.
5) by the glucose SERS detection substrate of above-mentioned preparation and configured various concentration (0.1,1,2,4,6mmol/L)
Glucose standards solution carry out reaction 0.5 hour after, detect SERS signal, obtain the SERS spectrogram of corresponding concentration.
6) spectrogram is handled, takes 1388cm-1Place's wave crest is normalized, further according to 1177cm-1Characteristic peak at three
Intensity obtain corresponding working curve with the relationship of concentration of glucose.
Embodiment 2
1) to 10mL, 90mL distilled water is added in the chlorauric acid solution of 2.4mmol/L, is heated to boiling, 1% citric acid is added
Sodium solution reaction 15min obtains nanogold.
2) nanogold is small with the 4- mercaptoaniline solution reaction at least 12 of various concentration (0.5mmol/L, 30 μ L) respectively
When, it is then centrifuged for washing.
3) to (0.6:25:50) sequentially adds silver nitrate solution, ascorbic acid, sodium hydroxide solution in proportion in 2), often
Temperature lower reaction 4 hours, nano silver shell is restored, the particle of Au@Ag nano-core-shell structure required for us is obtained, centrifugation is washed
It washs, taking lower layer's solution is Au Ag nano-core-shell structure colloid.
4) the Au@Ag nano-core-shell structure colloid prepared is taken, the 4- mercaptophenyl boronic acid that (1mmol/L, 10 μ L) are added is molten
Liquid reacts 2 hours, and control temperature is at 60 DEG C or so, centrifuge washing.
5) by the glucose SERS detection substrate of above-mentioned preparation and configured various concentration (0.1,1,2,4,6mmol/L)
Glucose standards solution carry out reaction 0.5 hour after, detect SERS signal, obtain the SERS spectrogram of corresponding concentration.
6) spectrogram is handled, takes 1388cm-1Place's wave crest is normalized, further according to 1177cm-1Characteristic peak at three
Intensity obtain corresponding working curve with the relationship of concentration of glucose.
Embodiment 3
1) to 10mL, 90mL distilled water is added in the chlorauric acid solution of 2.4mmol/L, is heated to boiling, 1% citric acid is added
Sodium solution reaction 15min obtains nanogold.
2) by nanogold 4- mercaptoaniline solution reaction at least 12 hours with various concentration (5mmol/L, 30 μ L) respectively,
It is then centrifuged for washing.
3) to (0.6:25:50) sequentially adds silver nitrate solution, ascorbic acid, sodium hydroxide solution in proportion in 2), often
Temperature lower reaction 4 hours, nano silver shell is restored, the particle of Au@Ag nano-core-shell structure required for us is obtained, centrifugation is washed
It washs, taking lower layer's solution is Au Ag nano-core-shell structure colloid.
4) the Au@Ag nano-core-shell structure colloid prepared is taken, the 4- mercaptophenyl boronic acid that (1mmol/L, 20 μ L) are added is molten
Liquid reacts 2 hours, and control temperature is at 60 DEG C or so, centrifuge washing.
5) by the glucose SERS detection substrate of above-mentioned preparation and configured various concentration (0.1,1,2,4,6mmol/L)
Glucose standards solution carry out reaction 0.5 hour after, detect SERS signal, obtain the SERS spectrogram of corresponding concentration.
6) spectrogram is handled, takes 1388cm-1Place's wave crest is normalized, further according to 1177cm-1Characteristic peak at three
Intensity obtain corresponding working curve with the relationship of concentration of glucose.
Embodiment 4
1) to 10mL, 90mL distilled water is added in the chlorauric acid solution of 2.4mmol/L, is heated to boiling, 1% citric acid is added
Sodium solution reaction 15min obtains nanogold.
2) nanogold is small with the 4- mercaptoaniline solution reaction at least 12 of various concentration (0.5mmol/L, 30 μ L) respectively
When, it is then centrifuged for washing.
3) to (0.6:25:50) sequentially adds silver nitrate solution, ascorbic acid, sodium hydroxide solution in proportion in 2), often
Temperature lower reaction 4 hours, nano silver shell is restored, the particle of Au@Ag nano-core-shell structure required for us is obtained, centrifugation is washed
It washs.
4) the Au@Ag nano-core-shell structure colloid prepared is taken, the 4- mercaptophenyl boronic acid that (1mmol/L, 30 μ L) are added is molten
Liquid reacts 2 hours, and control temperature is at 60 DEG C or so, centrifuge washing.
5) by the glucose SERS detection substrate of above-mentioned preparation and configured various concentration (0.1,1,2,4,6mmol/L)
Glucose standards solution carry out reaction 0.5 hour after, detect SERS signal, obtain the SERS spectrogram of corresponding concentration.
6) spectrogram is handled, takes 1388cm-1Place's wave crest is normalized, further according to 1077,1177,1584cm-1
The intensity of characteristic peak obtains corresponding working curve with the relationship of concentration of glucose at three.
Performance detection:
The glucose solution of corresponding amount is taken to be added in the urine of untreated Healthy People, same 4 method of embodiment preparation
Glucose SERS substrate carry out reaction 0.5 hour, survey SERS spectra.Above-mentioned spectrogram is handled, 1388cm is taken-1Locate wave crest
It is normalized, further according to 1177cm-1The intensity of characteristic peak obtains corresponding working curve with the relationship of concentration of glucose at three
(Fig. 9).
By Fig. 2 (embodiment 4), Fig. 3 (embodiment 1), Fig. 4 (embodiment 2) and Fig. 5 (embodiment 3) it is found that successfully having synthesized
The SERS substrate of Au@Ag nanoparticle；As shown in Figure 7, what the SERS detection substrate prepared using embodiment 4 was detected
The 1177cm that SERS spectra is made-1The working curve diagram that the peak intensity at place changes with concentration of glucose, linear correlation degree
It is high.As shown in Figure 9, the composite material prepared using embodiment 4 is detected different glucose solutions using SERS and is added to not as substrate
The urine of processed Healthy People, the 1177cm made using the SERS spectra measured-1The peak intensity at place is with concentration of glucose
The working curve diagram of variation, and compared with Fig. 7, it linearly coincide substantially, it follows that the detection method is by complicated in urine
The interference of substance is smaller, it is seen that the glucose SERS substrate of the invention has high sensitivity, and specificity is good, and reproducibility waits by force spies
Point.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (9)
1. a kind of SERS substrate of Au@Ag nanoparticle, it is characterised in that: the SERS substrate is a kind of with band 4- mercaptoaniline
Au@Ag nanoparticle be used as substrate, glucose capture molecule 4- mercaptophenyl boronic acid is modified in the Au@Ag nanoparticle surface
Composite material；For the Au@Ag nanoparticle using nanogold as core, nano silver is shell, between the nanogold core and nano silver shell
Diameter for 4- mercaptoaniline, the nanogold core is 23-27nm, and nano silver shell is with a thickness of 5-10nm.
2. a kind of preparation method of the SERS substrate of Au@Ag nanoparticle, which comprises the following steps:
(1) gold chloride is restored by the way that sodium citrate is added to obtain nanogold；
(2) upper 4- mercaptoaniline is carried in nanogold；
(3) the 4- mercaptoaniline carried is used to capture the silver ion in silver nitrate solution as presoma, is then added anti-bad
Hematic acid and sodium hydroxide restore nano silver shell, complete the preparation of Au@Ag nano-core-shell structure；
(4) 4- mercaptophenyl boronic acid is modified in the Au@Ag nano-core-shell structure of synthesis be used to specific recognition glucose molecule.
3. a kind of preparation method of the SERS substrate of Au@Ag nanoparticle according to claim 2, which is characterized in that step
(1) specific steps are as follows: 90mL distilled water is added into the chlorauric acid solution that 10mL concentration is 2.4mmol/L, is heated to boiling,
It is centrifugated after 1% sodium citrate solution reaction 15min is added, sediment is washed with distilled water and dehydrated alcohol, 80 DEG C of drying
Obtain nanogold.
4. a kind of preparation method of the SERS substrate of Au@Ag nanoparticle according to claim 2, which is characterized in that step
(2) specific steps are as follows: the nanogold of 15 μ L -40 μ L is added to the 4- mercaptoaniline solution of 0.05mmol/L-5mmol/L
After reaction 12-20 hours, centrifuge washing.
5. a kind of preparation method of the SERS substrate of Au@Ag nanoparticle according to claim 2, which is characterized in that step
(3) specific steps are as follows: to 20mL step (2) prepare nanogold in by volume for 0.5-0.8:20-35:40-55 successively
It is added the sodium hydroxide solution of the silver nitrate solution of 100mmol/L, 100mmol/L ascorbic acid and 100mmol/L, it is anti-under room temperature
It answers 4-6 hours, to go out nano silver shell in nanogold surface reduction, centrifuge washing, taking lower layer's solution is Au Ag nano core-shell
Structure colloid.
6. a kind of preparation method of the SERS substrate of Au@Ag nanoparticle according to claim 2, which is characterized in that step
(4) specific steps are as follows: the Au Ag nano-core-shell structure colloid for taking 20mL to prepare, 5 μ L-30 μ L concentration of addition are 1mmol/L
4- mercaptophenyl boronic acid solution, react under stirring condition 2-3 hour, reaction temperature is 60 DEG C -80 DEG C, and centrifuge washing is dried,
Obtain the SERS substrate of Au@Ag nanoparticle.
7. the detection method of glucose in a kind of urine, which comprises the following steps:
S1: by any one of claim 2-6 preparation glucose SERS substrate respectively with configured 5-6 various concentration gradient
Glucose standards solution carry out be mixed 0.5-1 hour after, drip on the cover slip, carried out using Reinshaw Raman spectrometer
The detection of Raman signal obtains the SERS spectrogram of corresponding concentration；
S2: taking corresponding SERS substrate individually to carry out Raman spectrum test, obtains substrate background Raman signal；
S3: use substrate background Raman signal that glucose standards solution Surface enhanced Raman spectroscopy is normalized as internal standard；
S4: glucose standards solution Raman spectrum spectral line relative intensity-concentration standard control working curve is established；
S5: detection unknown sample concentration: by the grape of any one of the sample with measurement of unknown concentration and claim 2-6 preparation
Sugared SERS substrate mixing, is then tested using laser Raman spectrometer, and carry out spectral strength normalized and obtain not
Know the glucose Surface enhanced Raman spectroscopy spectrogram of concentration；The concentration of glucose is calculated by formula.
8. according to claim 7 in a kind of urine glucose detection method, it is characterised in that: 5-6 concentration ladder
The concentration changing value of the glucose standards solution sample of degree is between 0.1-6 mmol/L.
9. according to claim 7 in a kind of urine glucose detection method, it is characterised in that: the unknown sample of step S5
For the urine of people.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810611514.9A CN109001176B (en) | 2018-06-14 | 2018-06-14 | Preparation method of SERS substrate of Au @ Ag nanoparticles and method for detecting glucose by using substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810611514.9A CN109001176B (en) | 2018-06-14 | 2018-06-14 | Preparation method of SERS substrate of Au @ Ag nanoparticles and method for detecting glucose by using substrate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109001176A true CN109001176A (en) | 2018-12-14 |
| CN109001176B CN109001176B (en) | 2021-08-24 |
Family
ID=64601769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810611514.9A Active CN109001176B (en) | 2018-06-14 | 2018-06-14 | Preparation method of SERS substrate of Au @ Ag nanoparticles and method for detecting glucose by using substrate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109001176B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991207A (en) * | 2019-04-25 | 2019-07-09 | 福建师范大学 | A kind of SERS sensor and its preparation and detection method for detecting the sandwich structure of tyrosinase |
| CN110320199A (en) * | 2019-07-30 | 2019-10-11 | 福建师范大学 | A method of based on histamine in the SERS technology detection flesh of fish |
| CN110376379A (en) * | 2019-08-19 | 2019-10-25 | 福建师范大学 | A kind of method that molecular engram combination quiet zone internal standard SERS technology high-precision detects CEA |
| CN110412014A (en) * | 2019-08-28 | 2019-11-05 | 济南大学 | Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection |
| CN110501322A (en) * | 2019-08-20 | 2019-11-26 | 广东食品药品职业学院 | A kind of flexible surface enhancing Raman substrate and its preparation method and application |
| CN110530842A (en) * | 2019-08-09 | 2019-12-03 | 温州大学 | Fe3O4@SiO2The preparation method and applications of/Au NPs particle |
| CN110749586A (en) * | 2019-11-05 | 2020-02-04 | 济南大学 | PMMA film-based self-assembled Au @ Ag dot matrix for detecting F-ions in solution |
| CN110987896A (en) * | 2019-11-11 | 2020-04-10 | 广西科技大学 | Trace amoxicillin detection method taking Ag @ Au as SERS substrate |
| CN111558727A (en) * | 2020-04-07 | 2020-08-21 | 西安工程大学 | Preparation method of clean bionic wool-gall-shaped nano structure |
| CN112285089A (en) * | 2020-09-23 | 2021-01-29 | 嘉兴学院 | Method for introducing internal standard molecules into surface enhanced Raman scattering substrate, substrate prepared by method and application of substrate |
| CN112414993A (en) * | 2020-11-17 | 2021-02-26 | 厦门大学 | Organic phosphorus Raman detection kit and method |
| CN112505017A (en) * | 2020-11-19 | 2021-03-16 | 福建师范大学 | Method for detecting IL-6 in blood based on SERS technology |
| WO2021046741A1 (en) * | 2019-09-11 | 2021-03-18 | 亳州市新健康科技有限公司 | Substrate material for high-efficiency surface-enhanced raman scattering and preparation method |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006065762A2 (en) * | 2004-12-13 | 2006-06-22 | University Of South Carolina | Surface enhanced raman spectroscopy using shaped gold nanoparticles |
| CN102426154A (en) * | 2011-08-24 | 2012-04-25 | 同济大学 | Preparation method for colorimetric sensor, product prepared by the same, and application of product |
| KR20140027786A (en) * | 2012-08-27 | 2014-03-07 | 강원대학교산학협력단 | Preparation method of silver nano-structure for surface enhanced raman scattering substrate and silver nano-structure thereby |
| CN103674925A (en) * | 2012-09-14 | 2014-03-26 | 北京大学 | 4-mercaptophenyl boronic acid-modified gold nanoparticles and method for detecting sugar marker on cell surface by using gold nanoparticles |
| CN104237174A (en) * | 2014-07-21 | 2014-12-24 | 南京邮电大学 | Method for detecting concanavalin based on single-particle Au@Ag core-shell structure |
| CN104237204A (en) * | 2014-10-16 | 2014-12-24 | 福建师范大学 | Preparation method of cellulose-nano-silver composite microsphere substrate for SERS (Surface Enhanced Raman Scattering) |
| CN104280441A (en) * | 2014-10-15 | 2015-01-14 | 甘肃省科学院传感技术研究所 | Method for preparing Au@Ag-PPY composite nanometer material and L-cysteine double-layer film immobilized glucose oxidase modified electrode |
| CN106124476A (en) * | 2016-06-21 | 2016-11-16 | 中山大学 | Based on surface enhanced raman spectroscopy and the glucose sensing approach of bi-molecular probe |
| CN106442675A (en) * | 2016-11-01 | 2017-02-22 | 济南大学 | Preparation and application of carcino-embryonic antigen electrochemical immunosensor based on Au@Ag@Au marker |
| US20170052123A1 (en) * | 2015-08-17 | 2017-02-23 | Lili He | Systems and methods for extraction and surface-enhanced raman spectroscopy detection of metal nanoparticles |
| CN106940313A (en) * | 2017-02-27 | 2017-07-11 | 未名环境分子诊断（常熟）有限公司 | A kind of illicit drugs inspection colorimetric sensor of the silver-colored gold-covered nano particle based on non-agglomerated |
| CN107328753A (en) * | 2017-07-14 | 2017-11-07 | 厦门稀土材料研究所 | The nanoparticle surface enhancing Raman spectrum quantitative analysis method of embedded internal standard molecule |
| US20180031546A1 (en) * | 2013-10-15 | 2018-02-01 | Board Of Trustees Of The University Of Arkansas | Nanocomposites and methods of making same |
| CN108051424A (en) * | 2017-11-30 | 2018-05-18 | 暨南大学 | SERS substrates, preparation method and applications based on shell surface |
-
2018
- 2018-06-14 CN CN201810611514.9A patent/CN109001176B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006065762A2 (en) * | 2004-12-13 | 2006-06-22 | University Of South Carolina | Surface enhanced raman spectroscopy using shaped gold nanoparticles |
| CN102426154A (en) * | 2011-08-24 | 2012-04-25 | 同济大学 | Preparation method for colorimetric sensor, product prepared by the same, and application of product |
| KR20140027786A (en) * | 2012-08-27 | 2014-03-07 | 강원대학교산학협력단 | Preparation method of silver nano-structure for surface enhanced raman scattering substrate and silver nano-structure thereby |
| CN103674925A (en) * | 2012-09-14 | 2014-03-26 | 北京大学 | 4-mercaptophenyl boronic acid-modified gold nanoparticles and method for detecting sugar marker on cell surface by using gold nanoparticles |
| US20180031546A1 (en) * | 2013-10-15 | 2018-02-01 | Board Of Trustees Of The University Of Arkansas | Nanocomposites and methods of making same |
| CN104237174A (en) * | 2014-07-21 | 2014-12-24 | 南京邮电大学 | Method for detecting concanavalin based on single-particle Au@Ag core-shell structure |
| CN104280441A (en) * | 2014-10-15 | 2015-01-14 | 甘肃省科学院传感技术研究所 | Method for preparing Au@Ag-PPY composite nanometer material and L-cysteine double-layer film immobilized glucose oxidase modified electrode |
| CN104237204A (en) * | 2014-10-16 | 2014-12-24 | 福建师范大学 | Preparation method of cellulose-nano-silver composite microsphere substrate for SERS (Surface Enhanced Raman Scattering) |
| US20170052123A1 (en) * | 2015-08-17 | 2017-02-23 | Lili He | Systems and methods for extraction and surface-enhanced raman spectroscopy detection of metal nanoparticles |
| CN106124476A (en) * | 2016-06-21 | 2016-11-16 | 中山大学 | Based on surface enhanced raman spectroscopy and the glucose sensing approach of bi-molecular probe |
| CN106442675A (en) * | 2016-11-01 | 2017-02-22 | 济南大学 | Preparation and application of carcino-embryonic antigen electrochemical immunosensor based on Au@Ag@Au marker |
| CN106940313A (en) * | 2017-02-27 | 2017-07-11 | 未名环境分子诊断（常熟）有限公司 | A kind of illicit drugs inspection colorimetric sensor of the silver-colored gold-covered nano particle based on non-agglomerated |
| CN107328753A (en) * | 2017-07-14 | 2017-11-07 | 厦门稀土材料研究所 | The nanoparticle surface enhancing Raman spectrum quantitative analysis method of embedded internal standard molecule |
| CN108051424A (en) * | 2017-11-30 | 2018-05-18 | 暨南大学 | SERS substrates, preparation method and applications based on shell surface |
Non-Patent Citations (4)
| Title |
|---|
| LUO，ZH等: ""Synthesis of p-aminothiophenol-embedded gold/silver core-shell nanostructures as novel SERS tags for biosensing applications", 《MICROCHIMICA ACTA》 * |
| SUN,D等: "Construction of highly sensitive surface-enhanced Raman scattering (SERS) nanosensor aimed for the testing of glucose in urine", 《RSC ADVANCES》 * |
| 刘向源等: "基于微流控芯片的层流技术和SERS方法定量葡萄糖", 《光谱学与光谱分析》 * |
| 李双双: "SERS在研究分子在银表面的吸附行为和结构变化中的应用", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991207A (en) * | 2019-04-25 | 2019-07-09 | 福建师范大学 | A kind of SERS sensor and its preparation and detection method for detecting the sandwich structure of tyrosinase |
| CN109991207B (en) * | 2019-04-25 | 2021-08-20 | 福建师范大学 | Sandwich-structured SERS sensor for detecting tyrosinase and preparation and detection methods thereof |
| CN110320199A (en) * | 2019-07-30 | 2019-10-11 | 福建师范大学 | A method of based on histamine in the SERS technology detection flesh of fish |
| CN110320199B (en) * | 2019-07-30 | 2021-08-27 | 福建师范大学 | Method for detecting histamine in fish meat based on SERS technology |
| CN110530842A (en) * | 2019-08-09 | 2019-12-03 | 温州大学 | Fe3O4@SiO2The preparation method and applications of/Au NPs particle |
| CN110376379A (en) * | 2019-08-19 | 2019-10-25 | 福建师范大学 | A kind of method that molecular engram combination quiet zone internal standard SERS technology high-precision detects CEA |
| CN110376379B (en) * | 2019-08-19 | 2022-07-12 | 福建师范大学 | Method for high-precision detection of CEA by combining molecular imprinting with silence area internal standard SERS technology |
| CN110501322A (en) * | 2019-08-20 | 2019-11-26 | 广东食品药品职业学院 | A kind of flexible surface enhancing Raman substrate and its preparation method and application |
| CN110412014A (en) * | 2019-08-28 | 2019-11-05 | 济南大学 | Application of the Surface enhanced Raman scattering paper base sensor in tumor-marker analyte detection |
| WO2021046741A1 (en) * | 2019-09-11 | 2021-03-18 | 亳州市新健康科技有限公司 | Substrate material for high-efficiency surface-enhanced raman scattering and preparation method |
| CN110749586A (en) * | 2019-11-05 | 2020-02-04 | 济南大学 | PMMA film-based self-assembled Au @ Ag dot matrix for detecting F-ions in solution |
| CN110987896A (en) * | 2019-11-11 | 2020-04-10 | 广西科技大学 | Trace amoxicillin detection method taking Ag @ Au as SERS substrate |
| CN110987896B (en) * | 2019-11-11 | 2022-06-03 | 广西科技大学 | Trace amoxicillin detection method taking Ag @ Au as SERS substrate |
| CN111558727A (en) * | 2020-04-07 | 2020-08-21 | 西安工程大学 | Preparation method of clean bionic wool-gall-shaped nano structure |
| CN112285089A (en) * | 2020-09-23 | 2021-01-29 | 嘉兴学院 | Method for introducing internal standard molecules into surface enhanced Raman scattering substrate, substrate prepared by method and application of substrate |
| CN112414993A (en) * | 2020-11-17 | 2021-02-26 | 厦门大学 | Organic phosphorus Raman detection kit and method |
| CN112505017A (en) * | 2020-11-19 | 2021-03-16 | 福建师范大学 | Method for detecting IL-6 in blood based on SERS technology |
| CN112505017B (en) * | 2020-11-19 | 2023-07-25 | 福建师范大学 | Method for detecting IL-6 in blood based on SERS technology |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109001176B (en) | 2021-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109001176A (en) | A kind of preparation method of the SERS substrate of Au@Ag nanoparticle and method using substrate detection glucose | |
| Achadu et al. | Sulfur-doped carbon dots@ polydopamine-functionalized magnetic silver nanocubes for dual-modality detection of norovirus | |
| Muhammad et al. | Fast probing of glucose and fructose in plant tissues via plasmonic affinity sandwich assay with molecularly-imprinted extraction microprobes | |
| CN111650370B (en) | Method and device for detecting novel coronavirus SARS-CoV-2 | |
| Li et al. | Highly selective determination of acid phosphatase in biological samples using a biomimetic recognition-based SERS sensor | |
| CN113237868B (en) | Ratio type detection method of surface enhanced Raman sensor based on graphene oxide to mycotoxin | |
| Li et al. | Silver-nanoparticle-based surface-enhanced Raman scattering wiper for the detection of dye adulteration of medicinal herbs | |
| Liu et al. | Ultrasensitive and facile detection of multiple trace antibiotics with magnetic nanoparticles and core-shell nanostar SERS nanotags | |
| CN106124476A (en) | Based on surface enhanced raman spectroscopy and the glucose sensing approach of bi-molecular probe | |
| Emami et al. | Comparison of gold nanoparticle conjugated secondary antibody with non-gold secondary antibody in an ELISA kit model | |
| CN114540022B (en) | Preparation of cellulose-based carbon quantum dot and application of cellulose-based carbon quantum dot in uric acid detection | |
| Zhang et al. | An electrochemical biosensor for the detection of aflatoxin B1 based on the specific aptamer and HCR biological magnification | |
| Wang et al. | Surface enhanced raman scattering active substrate based on hydrogel microspheres for pretreatment-free detection of glucose in biological samples | |
| CN107436301B (en) | 2, 6-dichlorophenol imprinted sensor based on surface enhanced Raman technology, and preparation method and application thereof | |
| CN106770178B (en) | A kind of kit quickly detecting diabetes using fingerprint | |
| CN109444240A (en) | A kind of electrochemistry immuno-sensing method established based on Prussian blue electrochemical immunosensor and based on the sensor and application | |
| WO2021188047A1 (en) | An electrochemical biosensor and method of fabricating the same | |
| CN113092553A (en) | Novel method for detecting glucose by enzyme-free glucose sensor | |
| Zhu et al. | Preparation of a multifunctional and ultrasensitive Au@ AgNPs-Van/PDMS film SERS substrate for rapid detection of foodborne pathogens in beef | |
| CN112461794A (en) | Long-range SPR sensor and preparation method thereof | |
| CN110006979B (en) | Diluteoside D electrochemical sensor based on nano CuO/Nafion membrane modified electrode | |
| Luo et al. | Catalytic hairpin assembly-mediated SERS biosensor for double detection of MiRNAs using gold nanoclusters-doped COF substrate | |
| CN108645840B (en) | Surface-enhanced Raman pesticide detection method based on gold-magnetic nano sandwich type | |
| CN113563222B (en) | Method for detecting hydrogen peroxide by surface enhanced Raman scattering based on silent region reporter molecule and application | |
| CN115436338A (en) | Method for detecting chlorpyrifos in tea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |