CN110396474A - A kind of stem cell filter separator - Google Patents
A kind of stem cell filter separator Download PDFInfo
- Publication number
- CN110396474A CN110396474A CN201910665277.9A CN201910665277A CN110396474A CN 110396474 A CN110396474 A CN 110396474A CN 201910665277 A CN201910665277 A CN 201910665277A CN 110396474 A CN110396474 A CN 110396474A
- Authority
- CN
- China
- Prior art keywords
- shell
- stem cell
- filter screen
- filter separator
- cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 43
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 claims abstract description 23
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000001914 filtration Methods 0.000 abstract description 5
- 238000004113 cell culture Methods 0.000 abstract description 4
- 239000000428 dust Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000005686 electrostatic field Effects 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 230000007717 exclusion Effects 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/03—Electric current
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Abstract
The invention discloses a kind of stem cell filter separators, by shell, sterilization structure, filter screen and baffle are constituted, when carrying out stem cell cultivation, stem cell is enclosed in cavity by sterilization structure, stem cell and culture solution are blocked in the top of filter screen and baffle by baffle, when needing to separate stem cell from culture solution, it only needs to slide in and out baffle relative to shell, culture solution is passed through from filter screen, stem cell is trapped on filter screen, completion is separated by filtration, when being cultivated and being separated, start the power supply of sterilization structure, power supply applies to the first conductive layer and the second conductive layer after voltage, an outside is formd to be positive, the electrostatic field that inside is negative, by the dust exclusion in air, moment after bacterial adsorption in cavity is killed, to obtain the effect for exempting from pollution.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of stem cell filter separators.
Background technique
Currently, stem cell refers to the initial cell with self-renewing and multi-lineage potential, it is the cells of origin of body,
The cell as one or more composition tissues or organ can be broken up under given conditions, stem cell needs when cultivating
It is filtered separation, existing filter separator has stem cell vulnerable to pollution.
Summary of the invention
The purpose of the present invention is to provide a kind of stem cell filter separators, it is intended to solve in the prior art stem cell vulnerable to
The problem of pollution.
To achieve the above object, a kind of stem cell filter separator that the present invention uses, including shell, sterilization structure, mistake
Strainer and baffle, the shell are placed on basal plane, and the inside of the shell has cavity, the one end of the cavity far from basal plane
Opening, the sterilization structure and the shell fasten, and are located at side of the shell far from basal plane, and the cavity is sealed
Firmly, the filter screen is fixedly connected with the shell, and position is in the cavity, and the baffle is slidably connected with the shell,
And it is contradicted with the filter screen, and be located at the side that the filter screen deviates from the sterilization structure；The sterilization structure includes exhausted
Edge layer, the first conductive layer, the second conductive layer and power supply, the insulating layer fasten on the housing, and are located at the filter screen
Side far from basal plane, first conductive layer are fixedly connected with the insulating layer, and are located at mistake described in the insulating layer distal side
The side of strainer, second conductive layer are fixedly connected with the insulating layer, and are located at the insulating layer and are led far from described first
The side of electric layer, the power supply and first conductive layer are electrically connected, and are electrically connected with second conductive layer.
Wherein, there is aperture, the aperture is located at the bottom of the shell, and is connected to the cavity on the shell.
Wherein, the filter separator further includes extracting device, and the extracting device is fixedly connected with the shell, and position
It is connected in the outside of the shell, and with the aperture.
Wherein, there is through-hole, the through-hole is connected to the cavity on the sterilization structure.
Wherein, the filter separator further includes syringe, and the syringe is located at the outside of the shell, and with it is described
Through-hole connection.
Wherein, the filter screen includes dictyosome and framework, and the framework is detachably connected with the shell, and is located at described
The inside of shell, the dictyosome are fixedly connected with the framework, and are located at the inside of the framework.
Wherein, the baffle includes plate body and limit plate, and the plate body and the dictyosome contradict, the plate body and the shell
Body is slidably connected, and stretches out the shell, and the limit plate is fixedly connected with the plate body, and position is in the cavity.
Wherein, the filter separator further includes weight block, and the weight block is fixedly connected with the dictyosome, and is located at institute
State the side that dictyosome deviates from the plate body.
Wherein, the weight block is located at the geometric center of the dictyosome.
Wherein, the weight block is spheroid form.
A kind of stem cell filter separator of the invention, by being made of shell, sterilization structure, filter screen and baffle, In
When carrying out stem cell cultivation, stem cell is enclosed in the cavity by the sterilization structure, by the baffle by stem cell and training
Nutrient solution is blocked in the top of the filter screen and the baffle, when needing to separate stem cell from culture solution, it is only necessary to
The baffle is slid in and out relative to the shell, culture solution is passed through from the filter screen, and stem cell is trapped within the mistake
It on strainer, completes to be separated by filtration, when being cultivated and being separated, starts the power supply of the sterilization structure, the power supply
After applying voltage to first conductive layer and second conductive layer, forms an outside and be positive, inside is negative quiet
Moment after bacterial adsorption in the cavity is killed the dust exclusion in air by electric field, exempts from pollution to obtain
Effect.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of separator of the present invention；
Fig. 2 is the schematic diagram of internal structure of inventive shell；
Fig. 3 is the structural schematic diagram of sterilization structure of the present invention；
Fig. 4 is the structural schematic diagram of baffle of the present invention；
Fig. 5 is the structural schematic diagram of sterilization structure of the present invention；
In figure: 100- filter separator, 10- shell, 11- cavity, 12- aperture, 20- sterilization structure, 21- through-hole, 22- are exhausted
Edge layer, the first conductive layer of 23-, the second conductive layer of 24-, 25- power supply, 30- filter screen, 31- dictyosome, 32- framework, 40- baffle,
41- plate body, 42- limit plate, 50- extracting device, 60- syringe, 70- weight block.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
In the description of the present invention, it is to be understood that, term " length ", " width ", "upper", "lower", "front", "rear",
The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside" is based on attached drawing institute
The orientation or positional relationship shown, is merely for convenience of description of the present invention and simplification of the description, rather than the dress of indication or suggestion meaning
It sets or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to limit of the invention
System.In addition, in the description of the present invention, the meaning of " plurality " is two or more, unless otherwise specifically defined.
Fig. 1 to Fig. 5 is please referred to, the present invention provides a kind of stem cell filter separators 100, including shell 10, disinfection knot
Structure 20, filter screen 30 and baffle 40, the shell 10 are placed on basal plane, and the inside of the shell 10 has cavity 11, described
Cavity 11 is far from the open at one end of basal plane, and the sterilization structure 20 is fastened with the shell 10, and it is separate to be located at the shell 10
The side of basal plane, and the cavity 11 is sealed, the filter screen 30 is fixedly connected with the shell 10, and is located at the cavity
In 11, the baffle 40 is slidably connected with the shell 10, and contradicts with the filter screen 30, and is located at the filter screen 30 and carries on the back
Side from the sterilization structure 20.
In the present embodiment, the shell 10 is placed on the interior that temperature is suitble to stem cell existence, by the baffle
40 slide to the lower section of the filter screen 30, and the baffle 40 blocks the mesh of the filter screen 30, the plate body 41 and described
Shell 10 and the sterilization structure 20 are all made of glass material, when carrying out stem cell cultivation, stem cell and culture solution
It is all located at the top of the filter screen 30 of the baffle 40, the cavity 11 is divided into two parts up and down by the baffle 40, when
It needs to separate stem cell from culture solution, it is only necessary to slide in and out the baffle 40 relative to the shell 10, train
Nutrient solution passes through the bottom for flowing into the cavity 11 from the filter screen 30, and stem cell is trapped on the filter screen 30, avoids
Stem cell is poured out and is filtered separation, when carrying out stem cell cultivation and being separated by filtration, starts the power supply 25, it is described
Power supply 25 selects impulse voltage generator, and the power supply 25 is fixedly connected with the insulating layer 22, pulse frequency when work
Within the scope of 20 to five ten hertz, the insulating layer 22 selects organic fiber, avoids generating excessively high accumulation of static electricity, the insulation
The thickness of layer 22 avoids first conductive layer 23 and second conductive layer 24 from contacting and open circuit occurs at one millimeter, and described the
One conductive layer 23 and second conductive layer 24 are all formed by metal wire knitted, first conductive layer 23 and second conduction
After layer 24 is powered, forms an outside and be positive, the electrostatic field that inside is negative, since the dust in air is usually all positively charged
Lotus, the same sex by the first conductive layer 23 repel barrier, avoid dust from entering in the cavity and pollute to stem cell, and empty
The intracavitary bacterium accidentally generated is all negatively charged, is adsorbed to the surface of outer conductive mesh, will under the action of high-voltage electrostatic field
Bacterium, virus, which are killed, leaves stem cell, to obtain the effect for exempting from pollution.
Further, there is aperture 12 on the shell 10, the aperture 12 is located at the bottom of the shell 10, and with institute
State the connection of cavity 11.In the embodiment of present embodiment, it is convenient for used culture solution by the aperture 12 from institute
It states shell 10 to discharge, culture solution is avoided to accumulate in the shell 10.
Further, the filter separator 100 further includes extracting device 50, the extracting device 50 and the shell 10
It is fixedly connected, and is located at the outside of the shell 10, and be connected to the aperture 12.In the embodiment of present embodiment, institute
Stating extracting device 50 can be used electronic pneumatic compression devices or manually piston, can produce the stream that negative-pressure sucking accelerates culture solution
It is dynamic.
Further, there is through-hole 21, the through-hole 21 is connected to the cavity 11 on the sterilization structure 20.In this reality
Apply in the embodiment of mode, by the through-hole 21 can be in order to being added to stem cell and culture solution.
Further, the filter separator 100 further includes syringe 60, and the syringe 60 is located at the shell 10
Outside, and be connected to the through-hole 21.It, can be to the filtering by the syringe 60 in the embodiment of present embodiment
The top of net 30 is inflated, and applies a pressure from the top of filter screen 30, is played and is accelerated culture solution from the filter screen 30 from wearing
The speed crossed.
Further, the filter screen 30 includes dictyosome 31 and framework 32, and the framework 32 and the shell 10 are detachable
Connection, and it is located at the inside of the shell 10, the dictyosome 31 is fixedly connected with the framework 32, and is located at the framework 32
Inside.In the embodiment of present embodiment, the framework 32 and 10 mortise-tenon joint of shell, the dictyosome 31 and the frame
Body 32 is fixedly connected, and the framework 32 is dismantled from the shell 10, can be cleaned in order to the filter screen 30.
Further, the baffle 40 includes plate body 41 and limit plate 42, and the plate body 41 is contradicted with the dictyosome 31, institute
It states plate body 41 to be slidably connected with the shell 10, and stretches out the shell 10, the limit plate 42 and the fixed company of the plate body 41
It connects, and is located in the cavity 11.In the embodiment of present embodiment, the upper surface of the plate body 41 will be on the dictyosome 31
Mesh block, the plate body 41 stretches out the part of the shell 10 and pulls out convenient for operator's hand force, the shell 10
When pulling out, it can finally be limited by the limit plate 42 and be detached from the shell 10.
Further, the filter separator 100 further includes weight block 70, and the weight block 70 and the dictyosome 31 are fixed
Connection, and it is located at the side that the dictyosome 31 deviates from the plate body 41.In the embodiment of present embodiment, when by the plate body
When 41 outside extraction, under the gravity of the weight block 70, the developed area of the filter screen 30 is bigger, can play and accelerate
The effect of filtering velocity degree.
Further, the weight block 70 is located at the geometric center of the dictyosome 31.In the embodiment of present embodiment
In, the uniformly expansion of dictyosome 31 can be made, avoid that the dictyosome 31 is caused to generate fold.
Further, the weight block 70 is spheroid form.It, can be to avoid stem cell in the embodiment of present embodiment
With culture solution weight block 70 surface detention.
Above disclosed is only a preferred embodiment of the present invention, cannot limit the power of the present invention with this certainly
Sharp range, those skilled in the art can understand all or part of the processes for realizing the above embodiment, and weighs according to the present invention
Benefit requires made equivalent variations, still belongs to the scope covered by the invention.
Claims (10)
1. a kind of stem cell filter separator, which is characterized in that
Including shell, sterilization structure, filter screen and baffle, the shell is placed on basal plane, and the inside of the shell has sky
Chamber, the cavity are fastened far from the open at one end of basal plane, the sterilization structure and the shell, and are located at the shell far from base
The side in face, and the cavity is sealed, the filter screen is fixedly connected with the shell, and position is in the cavity, described
Baffle is slidably connected with the shell, and contradicts with the filter screen, and is located at the filter screen away from the sterilization structure
Side；
The sterilization structure includes insulating layer, the first conductive layer, the second conductive layer and power supply, and the insulating layer is fastened on the shell
On body, and it is located at side of the filter screen far from basal plane, first conductive layer is fixedly connected with the insulating layer, and is located at
The side of filter screen described in the insulating layer distal side, second conductive layer are fixedly connected with the insulating layer, and are located at described
Side of the insulating layer far from first conductive layer, the power supply and first conductive layer are electrically connected, and with described second
Conductive layer is electrically connected.
2. stem cell filter separator as described in claim 1, which is characterized in that there is aperture on the shell, it is described small
Hole is located at the bottom of the shell, and is connected to the cavity.
3. stem cell filter separator as claimed in claim 2, which is characterized in that the filter separator further includes extracting to set
Standby, the extracting device is fixedly connected with the shell, and is located at the outside of the shell, and be connected to the aperture.
4. stem cell filter separator as described in claim 1, which is characterized in that have through-hole, institute on the sterilization structure
Through-hole is stated to be connected to the cavity.
5. stem cell filter separator as claimed in claim 4, which is characterized in that the filter separator further includes injection
Device, the syringe are located at the outside of the shell, and are connected to the through-hole.
6. stem cell filter separator as described in claim 1, which is characterized in that the filter screen includes dictyosome and framework,
The framework is detachably connected with the shell, and is located at the inside of the shell, and the dictyosome is fixedly connected with the framework,
And it is located at the inside of the framework.
7. stem cell filter separator as claimed in claim 6, which is characterized in that the baffle includes plate body and limit plate,
The plate body and the dictyosome contradict, and the plate body is slidably connected with the shell, and stretches out the shell, the limit plate and
The plate body is fixedly connected, and position is in the cavity.
8. stem cell filter separator as claimed in claim 7, which is characterized in that the filter separator further includes weight
Block, the weight block are fixedly connected with the dictyosome, and are located at the side that the dictyosome deviates from the plate body.
9. stem cell filter separator as claimed in claim 8, which is characterized in that the weight block is located at the several of the dictyosome
At what center.
10. stem cell filter separator as claimed in claim 9, which is characterized in that the weight block is spheroid form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910665277.9A CN110396474A (en) | 2019-07-23 | 2019-07-23 | A kind of stem cell filter separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910665277.9A CN110396474A (en) | 2019-07-23 | 2019-07-23 | A kind of stem cell filter separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110396474A true CN110396474A (en) | 2019-11-01 |
Family
ID=68325279
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910665277.9A Pending CN110396474A (en) | 2019-07-23 | 2019-07-23 | A kind of stem cell filter separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110396474A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110396473A (en) * | 2019-07-23 | 2019-11-01 | 中国人民解放军陆军军医大学第一附属医院 | A kind of stem cell multifunctional culture plate |
| US11447736B2 (en) | 2020-12-10 | 2022-09-20 | Alit Biotech (Shanghai) Co., Ltd. | Cell separation apparatus for bioreactor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007046501A1 (en) * | 2005-10-21 | 2007-04-26 | Kaneka Corporation | Stem cell separating material and method of separation |
| WO2008070083A2 (en) * | 2006-12-04 | 2008-06-12 | Keybay Pharma, Inc. | Filter media using peptide-based antimicrobials |
| US20160355774A1 (en) * | 2014-02-17 | 2016-12-08 | Asahi Kasei Kabushiki Kaisha | Cell culturing device |
| CN208917171U (en) * | 2018-09-26 | 2019-05-31 | 刘仁同 | A kind of filter separator for mesenchymal stem cells |
| CN210635998U (en) * | 2019-07-23 | 2020-05-29 | 中国人民解放军陆军军医大学第一附属医院 | Stem cell filtering separator |
-
2019
- 2019-07-23 CN CN201910665277.9A patent/CN110396474A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007046501A1 (en) * | 2005-10-21 | 2007-04-26 | Kaneka Corporation | Stem cell separating material and method of separation |
| US20090142835A1 (en) * | 2005-10-21 | 2009-06-04 | Kaneka Corporation | Stem cell separating material and method of separation |
| WO2008070083A2 (en) * | 2006-12-04 | 2008-06-12 | Keybay Pharma, Inc. | Filter media using peptide-based antimicrobials |
| US20160355774A1 (en) * | 2014-02-17 | 2016-12-08 | Asahi Kasei Kabushiki Kaisha | Cell culturing device |
| CN208917171U (en) * | 2018-09-26 | 2019-05-31 | 刘仁同 | A kind of filter separator for mesenchymal stem cells |
| CN210635998U (en) * | 2019-07-23 | 2020-05-29 | 中国人民解放军陆军军医大学第一附属医院 | Stem cell filtering separator |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110396473A (en) * | 2019-07-23 | 2019-11-01 | 中国人民解放军陆军军医大学第一附属医院 | A kind of stem cell multifunctional culture plate |
| US11447736B2 (en) | 2020-12-10 | 2022-09-20 | Alit Biotech (Shanghai) Co., Ltd. | Cell separation apparatus for bioreactor |
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