CN110393785B

CN110393785B - Composition for treating breast cancer complicated with depression, preparation method and application

Info

Publication number: CN110393785B
Application number: CN201910798246.0A
Authority: CN
Inventors: 孟盼; 王宇红; 朱青; 韩远山
Original assignee: Hunan University of Chinese Medicine
Current assignee: Hunan University of Chinese Medicine
Priority date: 2019-08-27
Filing date: 2019-08-27
Publication date: 2021-11-23
Anticipated expiration: 2039-08-27
Also published as: CN110393785A

Abstract

The invention relates to a composition for treating breast cancer complicated with depression, a preparation method and application thereof, wherein the composition comprises the following raw materials, by weight, 4-9 parts of cowherb seed, 6-10 parts of common cnidium fruit, 8-12 parts of American ginseng, 15-28 parts of motherwort, 3-9 parts of turmeric and 4-6 parts of rhodiola rosea; the invention adopts 6 Chinese medicinal component raw materials, adopts different effective component extraction methods according to treatment raw materials of breast cancer complicated with depression and aiming at different raw materials, exerts the effect of the medicament to the maximum extent, and has better curative effect compared with a direct decoction mode. On one hand, the content of effective components in the raw materials is improved, adverse components are removed, and the drug effect is improved by adopting the preparation method of the invention.

Description

Composition for treating breast cancer complicated with depression, preparation method and application

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a composition for treating breast cancer complicated with depression, a preparation method and application.

Background

In 1999, Maes proposed an inflammatory response hypothesis for depression, which theories that activation of the immune system is closely related to depression, i.e., the activated peripheral immune system leads to depression-related behavioral and neuroendocrine changes through the release of pro-inflammatory cytokines, a hypothesis also known as the cytokine theory for depression. The chronic inflammatory state of depression can be explained by cytokines secreted by monocytes and macrophages in the circulation. Studies have shown that IL-1 β (interleukin-1 β) concentration is increased and IL-6 concentration is decreased in cerebrospinal fluid of patients with depression, and IL-1 concentration correlates with the severity of depression. It has also been found in animal studies that chronic mild stress and acquisition of foot shock do not contribute to the central and peripheral IL-1 β elevation in depressed animals, and that injection of IL-1 receptor antagonists prior to stress can block the development of depressive-like behavior in animals. The above-mentioned arguments all indicate that there is a high incidence of immune activation in depression.

At present, whether the depression is a risk factor for tumorigenesis is not clear, but the poor prognosis of tumor patients has relevance. The hospitalization time of the tumor patients is prolonged, the life quality is reduced, the self-care capability is reduced, the emotion is unstable, and the suicide tendency is easy to generate. After radiotherapy or chemotherapy, tumor patients have fatigue, the score of depression scale is increased, and the levels of NF-k beta, IL-6 and IL-1 are increased. In conclusion, the development of BCRD is closely related to immune system disorders.

Among the numerous inflammatory cytokines, TNF- α, IL-1 β, IL-6, IL-8, and the like, play a major role. IL-6 can induce B cell differentiation and antibody production, and induce T cell activation proliferation and differentiation, and participate in immune response of organism, and is a promoter of inflammatory reaction. IL-8 can stimulate chemotaxis of neutrophils, T lymphocytes and eosinophils, promote degranulation of neutrophils, release elastase, damage endothelial cells, and lead microcirculation blood flow to be stagnated, tissues to be necrotic and organ functions to be damaged.

For breast cancer patients with concurrent depression, the currently common treatment strategy is the combination of chemotherapeutic drugs and antidepressant drugs. The taxol is a new anticancer drug extracted from the bark and leaves of jumble beads. The traditional Chinese medicine composition is widely used for treating ovarian cancer, breast cancer, non-small cell lung tumor, esophageal cancer and the like, and the anti-tumor mechanism of the traditional Chinese medicine composition is mainly through damaging DNA, changing the form of cellular nucleic acid, appearing apoptotic bodies and blocking cell division. The chemical drugs commonly used in clinical treatment of depression are mainly 5-HT reuptake inhibitors, including citalopram, fluoxetine, paroxetine and the like. The fluoxetine can highly selectively inhibit the reuptake of 5-hydroxytryptamine by presynaptic membranes, increase the content of 5-hydroxytryptamine in synaptic clefts, and simultaneously can act on postsynaptic membrane receptors to strengthen information transduction so as to achieve the effect of resisting depression.

The applicant filed a patent application with a Chinese patent application number of CN201811199437.7, and the raw materials of the patent application comprise the following components in parts by weight: 2-5 parts of radix bupleuri, 2-5 parts of angelica sinensis, 2-5 parts of radix paeoniae alba, 2-5 parts of poria cocos, 2-5 parts of bighead atractylodes rhizome, 1-3 parts of medlar, 1-3 parts of semen cuscutae, 3-10 parts of selfheal, 2-5 parts of rhizoma paridis, 1-3 parts of ginseng, 2-5 parts of turmeric, 0.5-2 parts of hypericum perforatum and 0.5-2 parts of honey-fried licorice root, and the traditional Chinese medicine is obtained by decocting the components in water.

Disclosure of Invention

The invention aims to provide a composition for treating breast cancer complicated with depression, a preparation method and application thereof.

The invention relates to a composition for treating breast cancer complicated with depression, which comprises the following raw materials, by weight, 4-9 parts of cowherb seed, 6-10 parts of common cnidium fruit, 8-12 parts of American ginseng, 15-28 parts of motherwort, 3-9 parts of turmeric and 4-6 parts of rhodiola rosea. Preferably, the traditional Chinese medicine comprises the following raw materials, by weight, 8 parts of cowherb seed, 9 parts of common cnidium fruit, 11 parts of American ginseng, 23 parts of motherwort, 8 parts of turmeric and 6 parts of rhodiola rosea.

The invention provides a preparation method of a composition for treating breast cancer complicated with depression, which comprises the following steps,

reflux-extracting semen Vaccariae with ethanol solution, drying to obtain total extract, sequentially extracting with petroleum ether, dichloromethane, ethyl acetate and n-butanol, drying each extracted product, and mixing to obtain semen Vaccariae extract;

pulverizing fructus Cnidii, adding biological enzyme, performing enzymolysis, filtering, collecting extractive solution, and concentrating to obtain fructus Cnidii extract;

ultrasonically extracting radix Panacis Quinquefolii with petroleum ether, drying, adding methanol solution, refluxing, extracting with n-butanol, and drying to obtain radix Panacis Quinquefolii extract;

mixing herba Leonuri with hydrochloric acid ethanol solution, ultrasonic treating, filtering, concentrating, and drying to obtain herba Leonuri extract;

pulverizing Curcuma rhizome, drying, extracting with ethanol solution, separating by silica gel column chromatography, eluting with chloroform, ethyl acetate and methanol mixture at a volume ratio of 38:1:1, after the color zone on the column is separated obviously, gradient eluting with chloroform, ethyl acetate and methanol solution at a volume ratio of 75:1:1, mixing the same samples, concentrating, and drying under reduced pressure to obtain Curcuma rhizome extract;

mixing radix Rhodiolae with ethanol solution, ultrasonic extracting, filtering, concentrating, and freeze drying to obtain radix Rhodiolae extract;

mixing the above semen Vaccariae extract, fructus Cnidii extract, radix Panacis Quinquefolii extract, herba Leonuri extract, Curcuma rhizome extract and radix Rhodiolae extract to obtain composition for treating breast cancer complicated with depression.

Preferably, in the preparation method of the cowherb seed extract, the volume concentration of ethanol is 75%, and the temperature of reflux extraction is 65 ℃.

Preferably, in the preparation method of the fructus cnidii extract, the biological enzyme is cellulase, the pH value of enzymolysis is 4-7, and the temperature is 50-60 ℃.

Preferably, in the preparation method of the American ginseng extract, the volume concentration of the methanol solution is 80%.

Preferably, the mass concentration of the hydrochloric acid in the hydrochloric acid ethanol solution is 0.05%.

Preferably, in the preparation method of the turmeric extract, the volume concentration of the ethanol solution is 65%, and the mass volume ratio of the turmeric and the ethanol is 1: 10.

Preferably, in the preparation method of the rhodiola rosea extract, the volume concentration of ethanol is 70%, and the mass volume ratio of the rhodiola rosea to the ethanol is 1: 10.

The invention provides application of a composition, and application of the composition in preparing a medicine or health-care product for treating breast cancer complicated by depression.

The traditional Chinese medicine composition has the beneficial effects that the traditional Chinese medicine composition adopts 6 traditional Chinese medicine component raw materials, different effective component extraction methods are adopted according to treatment raw materials of breast cancer complicated with depression aiming at different raw materials, the effect of the medicine is exerted to the maximum degree, and compared with a direct decoction mode, the medicine or health care product obtained by the preparation method has better curative effect. On one hand, the content of effective components in the raw materials is improved, adverse components are removed, and the drug effect is improved by adopting the preparation method of the invention.

Drawings

FIG. 1 is a standard curve of IL-2 in a serum sample.

FIG. 2 is a standard curve of IL-2 in tumor tissue samples.

FIG. 3 is a standard curve of IL-2 in hippocampal tissue samples.

FIG. 4 is a standard curve of IL-2 in a cortical tissue sample.

FIG. 5 is a standard curve for IL-6 in serum samples.

FIG. 6 is a standard curve for IL-6 in tumor tissue samples.

FIG. 7 Standard Curve for IL-6 in hippocampal tissue samples.

FIG. 8 is a standard curve for IL-6 in cortical tissue samples.

FIG. 9 is a standard curve for IL-10 in serum samples.

FIG. 10 is a standard curve of IL-10 in tumor tissue samples.

FIG. 11 is a standard curve of IL-10 in hippocampal tissue samples.

FIG. 12 is a standard curve of IL-10 in a cortical tissue sample.

FIG. 13 is a standard curve of IL-12 in serum samples.

FIG. 14 is a standard curve of IL-12 in tumor tissue samples.

FIG. 15 is a standard curve for IL-12 in hippocampal tissue samples.

FIG. 16 is a standard curve of IL-12 in a cortical tissue sample.

FIG. 17 is a standard curve of IFN-. gamma.for serum samples.

FIG. 18 is a standard curve of IFN-. gamma.for tumor tissue samples.

FIG. 19 is a standard curve of IFN-. gamma.for hippocampal tissue samples.

FIG. 20 is a standard curve of IFN-. gamma.for cortical tissue samples.

Detailed Description

Example 1

The raw materials of the invention comprise, by weight, 8 parts of cowherb seed, 9 parts of common cnidium fruit, 11 parts of American ginseng, 23 parts of motherwort, 8 parts of turmeric and 6 parts of rhodiola. Each of the raw material components was treated as follows.

Seed of cowherb: taking a certain amount of cowherb seed medicinal material, heating and refluxing with 10 times (volume weight ratio) of 75% (volume concentration) ethanol for 2 times, each time for 1.5h, filtering, combining liquid medicine, and freeze-drying to obtain the total extract. Extracting the total extract with petroleum ether, dichloromethane, ethyl acetate, and n-butanol sequentially. Freeze drying each part to obtain dry powder as semen Vaccariae extract.

Fructus cnidii: pulverizing dried fructus Cnidii, sieving with 40 mesh sieve, adding appropriate amount of biological enzyme into fructus Cnidii powder, adding distilled water according to a certain material-liquid ratio (1:5-1:10), performing enzymolysis at certain temperature and pH value, filtering, collecting extractive solution, and concentrating the obtained extractive solution.

American ginseng: ultrasonic extracting western radix Ginseng with appropriate amount of petroleum ether for 30min, taking out, volatilizing petroleum ether, adding 80% methanol, reflux extracting for 4 times (30 mL each time) for 30min each time. Combining the 4 extractive solutions, concentrating, evaporating to dryness, dissolving the residue with appropriate amount of distilled water, washing the container with distilled water, and combining the above solutions (about 30 mL); extracting with n-butanol for 3 times, each time with 10mL, mixing n-butanol extractive solutions, and washing with 20mL distilled water; the washed n-butanol solution was evaporated to dryness for further use.

Motherwort: putting motherwort coarse powder into a 3000mL round-bottom flask, adding 0.05% hydrochloric acid ethanol with the volume being 8 times that of the motherwort coarse powder, shaking to fully mix the motherwort coarse powder and the hydrochloric acid ethanol uniformly, soaking the motherwort coarse powder for 30min, then carrying out ultrasonic treatment for 40min, filtering, concentrating the filtrate under reduced pressure until the volume is about 100mL, putting the filtrate into a vacuum drying oven for drying, and powdering the dry paste for later use.

Turmeric: pulverizing Curcuma rhizome, drying (50 deg.C), grinding, and sieving with 60 mesh sieve. Adding 65% ethanol at solid-to-liquid ratio of 1:10, extracting for 2 times, each for 1.5 hr, and recovering. And then filling the silica gel column chromatography chromatographic column into the column by a dry method, eluting by adopting chloroform-ethyl acetate-methanol with the volume ratio of 38:1, controlling the flow rate at 80 drops/min, after the colored bands on the column are obviously separated, performing gradient elution by adopting chloroform-ethyl acetate-methanol with the volume ratio of 75:1, combining the same samples, concentrating, drying under reduced pressure, and pulverizing the dry paste for later use.

Rhodiola root: taking a proper amount of rhodiola rosea coarse powder, taking 70% ethanol as a solvent, extracting by adopting an ultrasonic-assisted thermal reflux method at the temperature of 60 ℃ and the solid-liquid ratio of 1:15g/mL, filtering, concentrating, freeze-drying, and pulverizing dry paste into powder for later use.

Example 2

Compared with the embodiment 1, the difference lies in that the raw material mixture ratio is different, and the raw material of the embodiment 2 comprises the following components, by weight, 4 parts of cowherb seed, 10 parts of common cnidium fruit, 12 parts of American ginseng, 15 parts of motherwort, 3 parts of turmeric and 4 parts of rhodiola rosea.

Example 3

Compared with the embodiment 1, the difference lies in that the raw material mixture ratio is different, and the raw material of the embodiment 3 comprises the following components, by weight, 9 parts of cowherb seed, 6 parts of common cnidium fruit, 8 parts of American ginseng, 28 parts of motherwort, 9 parts of turmeric and 6 parts of rhodiola rosea.

Experimental example 1

At present, whether the depression is a risk factor for tumorigenesis is not clear, but the poor prognosis of tumor patients has relevance. The hospitalization time of the tumor patients is prolonged, the life quality is reduced, the self-care capability is reduced, the emotion is unstable, and the suicide tendency is easy to generate. After radiotherapy or chemotherapy, the tumor patients have fatigue, the score of the depression scale is increased, and the levels of NF-k beta, IL-6, IL-1 and the like are increased. In conclusion, the development of BCRD is closely related to immune system disorders.

Relationship between cytokines and breast cancer with depression

Among the numerous inflammatory cytokines, TNF- α, IL-1 β, IL-6, IL-8, and the like, play a major role. IL-6 can induce B cell differentiation and antibody production, and induce T cell activation proliferation and differentiation, and participate in immune response of organism, and is a promoter of inflammatory reaction. IL-8 can stimulate chemotaxis of neutrophils, T lymphocytes and eosinophils, promote degranulation of neutrophils, release elastase, damage endothelial cells, and lead microcirculation blood flow to be stagnated and tissues to be necrotic, thereby causing organ function damage.

The IL-6 index in serum has important value for early diagnosis of breast cancer, evaluation of treatment effect and prognosis. A recent Meta analysis also showed that the concentration levels of IL-6 were significantly higher in depression patients than in the control group. The level of IL-6 in serum is presumed to be the biological basis of breast cancer and concomitant depressive disorder, and may have certain influence on the survival and quality of life of breast cancer patients. These indexes are selected to evaluate the effect of the medicine of the present invention on diseases.

1. EXAMPLE 1 Effect of compositions on anhedonia in BCRD mice

Compared with the blank group, the BCRD model group has obviously reduced sugar water consumption in the third week (P < 0.01 or P <0.05), and prompts the core anhedonia of the model animal; sugar water consumption significantly increased in the example 1 group compared to the model group when paclitaxel was combined with fluoxetine (P < 0.05). The suggestion shows that the BCRD mice obviously have anhedonia symptoms, and the example 1 can obviously improve the reaction of the BCRD mice to the anhedonia and relieve depression symptoms of the BCRD mice. The results are shown in Table 1.

Table 1 effect of composition on sugar water consumption in groups of mice: (

n＝10，％)

Note:^**P﹤0.01，^*p <0.05 compared with blank group;^#p <0.05 vs model group.

2. Example 1 Effect of compositions on autonomic behavior in mice

Compared with the blank group, the number of cross-erection lattices and the erection times of the mice in the BCRD model group are obviously reduced (P < 0.05); paclitaxel in combination with fluoxetine, the number of trellises in the group of example 1 was significantly increased compared to the model group (P < 0.01 or P < 0.05). It is suggested that the BCRD mice lack autonomous behavior in a novel environment, and the group of example 1 can increase the number of autonomous activities of the BCRD mice, and the effect of the combination of the BCRD mice and the taxol is more obvious. The results are shown in Table 2.

Table 2 effect of the composition of example 1 on the open field experiment in groups of mice: (

n＝10)

Note:^*p <0.05 compared to blank,^#P﹤0.01，^#p <0.05 vs model group.

3. Example 1 Effect of compositions on tumor marker levels in BCRD mice

Compared with the blank group, the tumor markers CA153 and CA125 of the mice in the BCRD model group are obviously increased (P < 0.01); compared with the model group, the tumor marker of the paclitaxel and fluoxetine combined group is obviously reduced in example 1 (P < 0.05). It is suggested that the composition of example 1 can assist paclitaxel to some extent and reduce the expression of tumor markers. The results are shown in Table 3.

TABLE 3 serum Breast cancer-associated marker levels in groups of mice

n＝5)

Note:^**p < 0.01 is compared with blank group;^##P<0.01，^#p <0.05 vs model group.

4. Changes in the series of immune factors

4.1 changes in IL-2

Compared with the blank group, the content of IL-2 in the serum, the hippocampus and the cortex of the mice in the BCRD model group is obviously increased, and the difference has statistical significance (P is less than 0.01 or P is less than 0.05). Compared with the model group, the content of IL-2 in the serum of the paclitaxel combined fluoxetine group in the example 1 group is obviously reduced, and the difference has statistical significance (P is less than 0.01 or P is less than 0.05); the content of IL-2 in serum, hippocampus and cortex of the BCRD model mouse is prompted to be increased remarkably, while the content of IL-2 in different tissues of the model mouse can be reduced remarkably in example 1, which indicates that the content of IL-2 in serum and brain of the model mouse can be reduced remarkably.

Compared with the model group, the paclitaxel and fluoxetine combined group in example 1 can obviously reduce the IL-2 content in the tumor, and the difference is statistically significant (P < 0.01 or P < 0.05). It is suggested that example 1 can assist the anticancer efficacy of chemotherapeutic agents, and the results are shown in Table 4, with the standard curves shown in FIGS. 1-4.

TABLE 4 changes in IL-2 levels in serum, tumor, hippocampus, cortex of each group

Note: in comparison with the blank set, the results,^**P﹤0.01，^*p is less than 0.05; in comparison with the set of models,^##P<0.01，^#P<0.05。

4.2 changes in IL-6

Compared with the blank group, the content of IL-6 in the serum, the hippocampus and the cortex of the mice in the BCRD model group is obviously increased, and the difference has statistical significance (P is less than 0.01 or P is less than 0.05). Compared with the model group, the content of IL-6 in the blood serum of the paclitaxel and fluoxetine combined group is obviously reduced in the example 1, and the difference is statistically significant (P < 0.01 or P < 0.05). The content of IL-6 in serum, hippocampus and cortex of the BCRD model mouse is prompted to be increased remarkably, while the content of IL-6 in different tissues of the model mouse can be reduced remarkably in example 1, which indicates that the content of IL-6 in serum and brain of the model mouse can be reduced remarkably.

Compared with the model group, the IL-6 content in the tumor tissues of the paclitaxel and fluoxetine combined group in example 1 is obviously reduced, and the difference is statistically significant (P < 0.01 or P < 0.05). It is suggested that example 1 may assist the anticancer efficacy of chemotherapeutic drugs. The content of IL-6 is found to be several times higher than that of other immune factors in four different tissues, and the detection is good. The results are shown in Table 5. The standard curves are shown in fig. 5-8.

TABLE 5 changes in IL-6 levels in serum, tumor, hippocampus, cortex of each group

4.3 changes in IL-10

Compared with the blank group, the content of IL-10 in the serum, the hippocampus and the cortex of the mice in the BCRD model group is obviously increased, and the difference has statistical significance (P is less than 0.01 or P is less than 0.05). Compared with the model group, the IL-10 content in the blood serum of the paclitaxel and fluoxetine combined group in example 1 is obviously reduced, and the difference has statistical significance (P < 0.01 or P < 0.05). The content of IL-10 in the serum, hippocampus and cortex of the mice in the BCRD model group is prompted to be obviously increased, while the content of IL-10 in different tissues of the mice in the BCRD model group can be obviously reduced in example 1, which indicates that the content of IL-10 in the serum and the brain of the mice in the model group can be obviously reduced.

Compared with the model group, the IL-10 content in the tumor tissues of the paclitaxel and fluoxetine combined group in example 1 is obviously reduced, and the difference is statistically significant (P < 0.01 or P < 0.05). It is suggested that example 1 may assist the anticancer efficacy of chemotherapeutic drugs. The results are shown in Table 6. The standard curves are shown in fig. 9-12.

TABLE 6 changes in IL-10 levels in serum, tumor, hippocampus, cortex of each group

4.4 changes in IL-12

Compared with a blank group, the content of IL-12 in the serum, the hippocampus and the cortex of the mice in the BCRD model group is obviously increased, and the difference has statistical significance (P is less than 0.01 or P is less than 0.05); example 1, the serum IL-12 content of the paclitaxel and fluoxetine combination group is obviously reduced, and the difference has statistical significance (P < 0.01 or P < 0.05). The result shows that the content of IL-12 in serum, hippocampus and cortex of the BCRD model mouse is increased remarkably, while the content of IL-12 in different tissues of the model mouse can be reduced remarkably by the example 1.

Compared with the model group, the content of IL-12 in the tumor tissues of the paclitaxel and fluoxetine combined group in example 1 is obviously reduced, and the difference is statistically significant (P < 0.01 or P < 0.05). It is suggested that example 1 may assist the anticancer efficacy of chemotherapeutic drugs. The results are shown in Table 7. The standard curves are shown in fig. 13-16.

TABLE 7 changes in IL-12 levels in serum, tumor, hippocampus, cortex of each group

5.6 changes in IFN-. gamma.

Compared with the blank group, the content of IFN-gamma in the serum, the hippocampus and the cortex of the mice in the BCRD model group is obviously increased, and the difference has statistical significance (P is less than 0.01 or P is less than 0.05). Compared with the model group, the content of IFN-gamma in the blood serum of the paclitaxel and fluoxetine combined group in the example 1 is obviously reduced, and the difference has statistical significance (P < 0.01 or P < 0.05). The content of IFN-gamma in serum, hippocampus and cortex of the BCRD model mouse is obviously increased, while the content of IFN-gamma in different tissues of the model mouse can be obviously reduced in example 1.

Compared with the model group, the content of IFN-gamma in the tumor tissues of the paclitaxel and fluoxetine combined group in example 1 is obviously reduced, and the difference is statistically significant (P < 0.01 or P < 0.05). It is suggested that example 1 may assist the anticancer efficacy of chemotherapeutic drugs. The results are shown in Table 8. The standard curves are shown in FIGS. 17-20.

TABLE 8 changes in IFN-. gamma.content in serum, tumor, hippocampus, cortex of each group

the composition has good effects of resisting cancer and relieving depression, and research results show that the composition can increase the sugar water monophagia degree and the autonomous activity of a model mouse, reduce the contents of tumor markers CA153 and CA125, and prompt that the composition has the effects of resisting cancer and relieving depression from the aspect of characterization and judgment. Furthermore, the suspension chip is adopted to carry out systematic screening on the content of the characteristic inflammatory factors in 6 different tissues such as mouse serum, tumor, hippocampus, cortex and the like, and the result shows that: (1) the content of inflammatory factors in different tissues is obviously different, wherein the content of IL-1b, IL-12, IFN-y and IL-6 in serum is obviously higher than that of hippocampus, tumor and cortex; the content of IL-6, IL-2 and IL-10 in the hippocampus is higher than that of inflammatory factors in tumor, cortex and serum; (2) IL-1b has very low concentration in four different tissues, and even in a suspension chip with higher precision, a phenomenon that part of samples are difficult to detect exists; (3) the IL-6 in the factor has higher expression content in four different tissues; (4) the composition can obviously reduce the contents of IL-6, IL-12, IL-10 and IFN-y in serum, hippocampus, cortex and tumor, and has no influence on the expression of IL-1 b.

Claims

1. A composition for treating breast cancer complicated with depression is characterized by being prepared from the following raw materials, by weight, 4-9 parts of cowherb seed, 6-10 parts of common cnidium fruit, 8-12 parts of American ginseng, 15-28 parts of motherwort, 3-9 parts of turmeric and 4-6 parts of rhodiola rosea.

2. The composition for treating breast cancer complicated with depression as claimed in claim 1, which is prepared from the following raw materials, by weight, 8 parts of cowherb seed, 9 parts of common cnidium fruit, 11 parts of American ginseng, 23 parts of motherwort herb, 8 parts of turmeric and 6 parts of rhodiola rosea.

3. A method for preparing a composition for the treatment of breast cancer complicated with depression as claimed in claim 1 or 2, comprising the steps of,

4. The method according to claim 3, wherein the cowherb seed extract is prepared by a method in which the ethanol concentration is 75% by volume and the reflux extraction temperature is 65 ℃.

5. The method of claim 3, wherein the cnidium monnieri extract is prepared by a method in which the bio-enzyme is cellulase, the pH of the enzymatic hydrolysis is 4-7, and the temperature is 50-60 ℃.

6. The method of claim 3, wherein the American ginseng extract is prepared by a method in which a methanol solution has a concentration of 80% by volume.

7. The method according to claim 3, wherein the hydrochloric acid is contained in an ethanol solution of hydrochloric acid at a concentration of 0.05% by mass.

8. The method according to claim 3, wherein the turmeric extract is prepared by a method in which the ethanol solution has a volume concentration of 65% and the mass-to-volume ratio of turmeric and ethanol is 1: 10.

9. The preparation method of claim 3, wherein the rhodiola rosea extract is prepared by a method in which the volume concentration of ethanol is 70%, and the mass volume ratio of rhodiola rosea to ethanol is 1: 10.

10. Use of a composition according to claim 1 or 2, for the preparation of a medicament for the treatment of depression associated with breast cancer.